3.4.21.9 A4-mutant trypsinogen + H2O - Homo sapiens ? - ? 384027 3.4.21.9 AAVERW-streptavidin-R-phycoerythrin conjugate + H2O peptide conjugated to fluorescent probe streptavidin-conjugated phycoerythrin Homo sapiens AAVERW + streptavidin-R-phycoerythrin conjugate - ? 394182 3.4.21.9 AhR6-C/EBP hybrid of the AhR basic region and C/EBP leucine zipper. Reaction under standard conditions (at 37°C in 50 mM Tris buffer, pH 7.6) is rapid and somewhat nonspecific. After just 15 min incubation, 82% of the protein has been cleaved at multiple sites, prolonged incubation causes further degradation of cleavage products synthetic construct ? - ? 404249 3.4.21.9 alpha-benzoyl-DL-Arg-4-nitroanilide + H2O - Struthio camelus alpha-benzoyl-DL-Arg + 4-nitroaniline - ? 74467 3.4.21.9 angiotensin II + H2O - Bos taurus ? - ? 16269 3.4.21.9 APFDDDDKIVGG + H2O N-terminal dodecapeptides of human cationic trypsinogen Homo sapiens ? - ? 394235 3.4.21.9 APFDDDDKIVGG + H2O N-terminal dodecapeptides of human cationic trypsinogen Bos taurus ? - ? 394235 3.4.21.9 APFDDDDRIVGG + H2O - Bos taurus ? - ? 453060 3.4.21.9 APFDDDGKIVGG + H2O N-terminal dodecapeptides of human pancreatitis-associated mutant variant of trypsinogen Bos taurus ? - ? 394236 3.4.21.9 APFDDDGRIVGG + H2O N-terminal dodecapeptides of human cationic tryosinogen Bos taurus ? - ? 394237 3.4.21.9 benzoyl-Arg ethyl ester + H2O - Homo sapiens benzoyl-Arg + ethanol - ? 36926 3.4.21.9 benzoyl-Arg ethyl ester + H2O - Sus scrofa benzoyl-Arg + ethanol - ? 36926 3.4.21.9 benzoyl-Arg ethyl ester + H2O - Bos taurus benzoyl-Arg + ethanol - ? 36926 3.4.21.9 benzyl-L-Arg-2-naphthylamide + H2O - Homo sapiens ? - ? 431656 3.4.21.9 benzyl-L-Arg-2-naphthylamide + H2O - Bos taurus ? - ? 431656 3.4.21.9 benzyloxycarbonyl-L-alanine-X-L-arginine-p-nitroanilide + H2O Z-Ala-X-Arg-pNA Homo sapiens p-nitroaniline + benzyloxycarbonyl-L-alanine-X-L-arginine - ? 394274 3.4.21.9 benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl 7-amide + H2O - Sus scrofa ? - ? 74469 3.4.21.9 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O - Oryzias latipes ? - ? 95073 3.4.21.9 Boc-Glu(OBzl)-Ala-Arg-4-methylcoumaryl-7-amide + H2O - Oryzias latipes Boc-Glu(OBzl)-Ala-Arg + 7-amino-4-methylcoumarin - ? 394517 3.4.21.9 bovine trypsinogen + H2O - Bos taurus ? - ? 384364 3.4.21.9 DDDK-SA-PE + H2O Asp-Asp-Asp-Asp-Lys-fluorescent probe streptavidin-conjugated phycoerythrin Homo sapiens DDDK + SA-PE - ? 394312 3.4.21.9 DDRRAG-SA-PE + H2O peptide conjugated to fluorescent probe streptavidin-conjugated phycoerythrin Homo sapiens DDRRAG + SA-PE - ? 394313 3.4.21.9 DRARVW-SA-PE + H2O peptide conjugated to fluorescent probe streptavidin-conjugated phycoerythrin Homo sapiens DRARVW + SA-PE - ? 394320 3.4.21.9 egg white lysozyme + H2O egg white lysozyme from hen Bos taurus ? - ? 74465 3.4.21.9 EYDRQL-SA-PE + H2O peptide conjugated to fluorescent probe streptavidin-conjugated phycoerythrin Homo sapiens EYDRQL + SA-PE - ? 394325 3.4.21.9 formyl-Ala-Phe-Arg-4-nitroanilide + H2O - Homo sapiens 4-nitroaniline + formyl-Ala-Phe-Arg - ? 394329 3.4.21.9 formyl-Ala-Phe-Arg-4-nitroanilide + H2O a chromogenic peptide substrates Homo sapiens ? - ? 418628 3.4.21.9 formyl-Ala-Phe-Arg-4-nitroanilide + H2O a chromogenic peptide substrates Bos taurus ? - ? 418628 3.4.21.9 formyl-Ala-Phe-Lys-4-nitroanilide + H2O - Homo sapiens 4-nitroaniline + formyl-Ala-Phe-Lys - ? 394330 3.4.21.9 formyl-Ala-Phe-Lys-4-nitroanilide + H2O a chromogenic peptide substrates Homo sapiens ? - ? 418629 3.4.21.9 formyl-Ala-Phe-Lys-4-nitroanilide + H2O a chromogenic peptide substrates Bos taurus ? - ? 418629 3.4.21.9 fusion protein Trx/hEGF + H2O the fusion protein Trx/hEGF contains an inter-domain enteropeptidase recognition site Homo sapiens ? - ? 366199 3.4.21.9 fusion protein Trx/hIL-13 + H2O the fusion protein Trx/hIL-13 contains an inter-domain enteropeptidase recognition site Homo sapiens ? - ? 366198 3.4.21.9 G5DKF(NO2)G + H2O - Homo sapiens ? - ? 384744 3.4.21.9 G5DKF(NO2)G + H2O - Bos taurus ? - ? 384744 3.4.21.9 GD4K 2-naphthylamide + H2O - Homo sapiens ? - ? 418643 3.4.21.9 GD4K 7-amido-4-methylcoumarin + H2O - Homo sapiens ? - ? 418644 3.4.21.9 GD4KF(NO2)G + H2O - Homo sapiens ? - ? 384759 3.4.21.9 GD4KF(NO2)G + H2O - Bos taurus ? - ? 384759 3.4.21.9 GD4KNfa + H2O - Bos taurus ? - ? 453057 3.4.21.9 GD4R-4-nitroanilide + H2O - Homo sapiens ? - ? 431869 3.4.21.9 GDDDDK-2-naphthylamide + H2O - Homo sapiens GDDDDK + 2-naphthylamine - ? 431870 3.4.21.9 GDDDDK-2-naphthylamide + H2O specific substrate Oryzias latipes GDDDDK + 2-naphthylamine - ? 431870 3.4.21.9 GDDDDK-4-nitroanilide + H2O - Homo sapiens GDDDDK + 4-nitroaniline - ? 431872 3.4.21.9 glutathione S-transferase-enterokinase + H2O GST-EK harboring an EK site Bos taurus enterokinase + glutathione S-transferase - ? 394340 3.4.21.9 Gly-(L-Asp)4-L-Lys-2-naphthylamide + H2O - Bos taurus Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine - ? 431907 3.4.21.9 Gly-(L-Asp)4-L-Lys-2-naphthylamide + H2O - Bos taurus Gly-(L-Asp)4-L-Lys + 2-naphthylamine - ? 449984 3.4.21.9 Gly-(L-Asp)4-L-Lys-2-naphthylamide + H2O - Homo sapiens Gly-(L-Asp)4-L-Lys + 2-naphthylamine - ? 449984 3.4.21.9 Gly-Asp-ASp-Asp-Asp-2-naphthylamide + H2O - Sus scrofa Gly-Asp-ASp-Asp-Asp + 2-naphthylamine - ? 453059 3.4.21.9 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O - Homo sapiens Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine - ? 74456 3.4.21.9 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O - Sus scrofa Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine - ? 74456 3.4.21.9 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O - Bos taurus Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine - ? 74456 3.4.21.9 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O - Oryzias latipes Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine - ? 74456 3.4.21.9 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O GD4K-na Homo sapiens Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine - ? 74456 3.4.21.9 Gly-Asp-Asp-Asp-Asp-Lys-Ile-Val-Gly-Gly + H2O - Bos taurus Gly-Asp-Asp-Asp-Asp-Lys + Ile-Val-Gly-Gly - ? 418683 3.4.21.9 Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide + H2O GD4K-na Bos taurus naphthylamine + Gly-Asp-Asp-Asp-Asp-Lys - ? 394345 3.4.21.9 Gly-Gly-Asp-Asp-Asp-Lys-Ile-Val-Gly-Gly + H2O - Bos taurus Gly-Gly-Asp-Asp-Asp-Lys + Ile-Val-Gly-Gly - ? 418684 3.4.21.9 Gly-Gly-Gly-Asp-Asp-Lys-Ile-Val-Gly-Gly + H2O - Bos taurus Gly-Gly-Gly-Asp-Asp-Lys + Ile-Val-Gly-Gly - ? 418685 3.4.21.9 Gly-Gly-Gly-Gly-Asp-Lys-Ile-Val-Gly-Gly + H2O - Bos taurus Gly-Gly-Gly-Gly-Asp-Lys + Ile-Val-Gly-Gly - ? 418686 3.4.21.9 Gly-L-Asp-L-Asp-L-Asp-L-Asp-L-Lys-2-naphthylamide + H2O - Homo sapiens Gly-L-Asp-L-Asp-L-Asp-L-Asp-L-Lys + 2-naphthylamine - ? 449989 3.4.21.9 glycyl-tetra-L-aspartyl-L-lysine beta-naphthylamide + H2O - Homo sapiens glycyl-tetra-L-aspartyl-L-lysine + beta-naphthylamine - ? 384833 3.4.21.9 GST-GFPuv + H2O harboring an EK site between GST and the green fluorescent protein GFPuv Bos taurus GST + GFPuv - ? 394354 3.4.21.9 GST-vasostatin fusion protein + H2O - Bos taurus ? - ? 384841 3.4.21.9 human cationic trypsinogen + H2O - Homo sapiens ? - ? 384890 3.4.21.9 human cationic trypsinogen + H2O - Bos taurus ? - ? 384890 3.4.21.9 human thioredoxin fused human NT-proCNP(1-50) + H2O 22kDA substrate Sus scrofa cleavage products of human thioredoxin fused human NT-proCNP(1-50) 21 and 16 kDa cleavage products using the recombinant enzyme, multiple cleavage products using the native enzyme ? 394363 3.4.21.9 interferon-alpha2a + H2O recombinantly expressed substrate Sus scrofa ? - ? 418724 3.4.21.9 interferon-alpha2b + H2O recombinantly expressed substrate Sus scrofa ? - ? 418725 3.4.21.9 LTAEEKA + H2O - Homo sapiens ? - ? 385099 3.4.21.9 LTAEEKA + H2O Hb 2-8 Bos taurus ? - ? 385099 3.4.21.9 LTAEEKAAV + H2O - Homo sapiens ? - ? 385100 3.4.21.9 Lys-2-naphthylamide + H2O - Sus scrofa Lys + 2-naphthylamine - ? 36318 3.4.21.9 Melittin + H2O - Homo sapiens ? - ? 16535 3.4.21.9 MHGERM-SA-PE + H2O peptide conjugated to fluorescent probe streptavidin-conjugated phycoerythrin Homo sapiens MHGERM + SA-PE - ? 394417 3.4.21.9 MLTAEEKAA + H2O Hb 1-9 Bos taurus ? - ? 385193 3.4.21.9 additional information the enteropeptidase heavy chain has little influence on the recognition of small peptides, but strongly influences macromolecular substrate recognition Bos taurus ? - ? 89 3.4.21.9 additional information the catalytic subunit retains the restricted specificity of intact enterokinase but the rate of activation of trypsinogen is much slower Bos taurus ? - ? 89 3.4.21.9 additional information acidic residues at the P2, P3 and/or P4 position are especially favorable for maximal activity, but are not absolutely necessary Sus scrofa ? - ? 89 3.4.21.9 additional information the highly specific protease cleaves immediately after the carboxyl-terminal residue of the (Asp)4-Lys recognition sequence Bos taurus ? - ? 89 3.4.21.9 additional information susceptible bonds are either Lys or Arg. The preceding acidic residues could be either Asp, Glu, or carboxymethyl cysteine Bos taurus ? - ? 89 3.4.21.9 additional information cleaves after Lys residues of peptidyl substrates that resemble trypsinogen activation peptides such as Val-(Asp)4-Lys Bos taurus ? - ? 89 3.4.21.9 additional information Val-(Asp)2-(Ala)2-Lys-Ile-Val-Gly, Val-Ala-Asp-(Ala)2-Lys-Ile-Val-Gly, Val-(Asp)4-Lys-Ile-Val-Gly, Val-(Asp)4-Ala-Ile-Val-Gly and Val-(Asp)4-Glu-Ile-Val-Gly are not hydrolyzed Bos taurus ? - ? 89 3.4.21.9 additional information Val-(Asp)2-(Ala)2-Lys-Ile-Val-Gly, Val-Ala-Asp-(Ala)2-Lys-Ile-Val-Gly, Val-(Asp)4-Lys-Ile-Val-Gly, Val-(Asp)4-Ala-Ile-Val-Gly and Val-(Asp)4-Glu-Ile-Val-Gly are not hydrolyzed Sus scrofa ? - ? 89 3.4.21.9 additional information enterokinase light chain is a serine protease that recognizes Asp-Asp-Asp-Asp-Lys, D4K, sequence and cleaves the C-terminal peptide bond of the lysine residue Bos taurus ? - ? 89 3.4.21.9 additional information enteropeptidase cleaves the C-terminal end of the substrate recognition sequence Asp-Asp-Asp-Asp-Lys, D4K. Usage of GD4K-conjugated 7-amino-4-methylcoumarin or GD4K-conjugated 2-naphthylamine as a fluorogenic substrates in the assay for enteropeptidase Homo sapiens ? - ? 89 3.4.21.9 additional information high degree of cleavage specificity is exhibited by enteropeptidase Homo sapiens ? - ? 89 3.4.21.9 additional information high degree of cleavage specificity is exhibited by enteropeptidase Bos taurus ? - ? 89 3.4.21.9 MSGERM-SA-PE + H2O peptide conjugated to fluorescent probe streptavidin-conjugated phycoerythrin Homo sapiens MSGERM + SA-PE - ? 394418 3.4.21.9 MUC1-IgG Fc + H2O fusion protein Bos taurus MUC1 + IgG Fc - ? 394419 3.4.21.9 mucin 1-IgG2a Fc + H2O cleavage of the mucin fusion protein Bos taurus mucin 1 + ? - ? 412464 3.4.21.9 N-alpha-benzyloxycarbonyl-L-lysine thiobenzyl ester + H2O - Homo sapiens N-alpha-benzyloxycarbonyl-L-lysine + benzylmercaptane - ? 385256 3.4.21.9 N-alpha-benzyloxycarbonyl-L-lysine thiobenzyl ester + H2O - Homo sapiens N-alpha-benzyloxycarbonyl-L-lysine + phenylmethanethiol - ? 453058 3.4.21.9 N2-benzoyl-L-Arg ethyl ester + H2O - Bos taurus ? - ? 454013 3.4.21.9 Nalpha-acetyltrypsinogen + H2O - Homo sapiens Nalpha-acetylactivation peptides + trypsin - ? 74458 3.4.21.9 Nalpha-acetyltrypsinogen + H2O - Sus scrofa Nalpha-acetylactivation peptides + trypsin - ? 74458 3.4.21.9 Nalpha-acetyltrypsinogen + H2O - Bos taurus Nalpha-acetylactivation peptides + trypsin - ? 74458 3.4.21.9 PrAD4KP26 + H2O fusion protein containing a modified protein A as a carrier and recoverin as a target protein Bos taurus ? - ? 385577 3.4.21.9 Pro-Phe-Arg-4-methylcoumaryl-7-amide + H2O - Oryzias latipes 7-amino-4-methylcoumarin + Pro-Phe-Arg - ? 394479 3.4.21.9 protein C inhibitor + H2O - Homo sapiens ? - ? 362136 3.4.21.9 S-alkylated soybean trypsin inhibitor + H2O limited proteolysis Bos taurus ? - ? 74464 3.4.21.9 S-carboxyamidomethyl derivative of bovine serum albumin + H2O bovine serum albumin is resistant in its native state, somewhat susceptible as the S-carboxyamidomethyl derivative and highly susceptible as the S-carboxymethyl derivative Bos taurus ? - ? 74463 3.4.21.9 SERAAAG-SA-PE + H2O peptide conjugated to fluorescent probe streptavidin-conjugated phycoerythrin Homo sapiens SERAAAG + SA-PE - ? 394503 3.4.21.9 SGDRMW-SA-PE + H2O peptide conjugated to fluorescent probe streptavidin-conjugated phycoerythrin, exhibits a 17 fold faster cleavage time than DDDK Homo sapiens SGDRMW + SA-PE - ? 394504 3.4.21.9 SGERMMG-SA-PE + H2O peptide conjugated to fluorescent probe streptavidin-conjugated phycoerythrin Homo sapiens SGERMMG + SA-PE - ? 394505 3.4.21.9 t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin + H2O - Oryzias latipes t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg + 7-amino-4-methylcoumarin - ? 432426 3.4.21.9 tert-butoxycarbonyl-Gln-Ala-Arg-4-methylcoumarin 7-amide + H2O weak activity Sus scrofa ? - ? 74471 3.4.21.9 tert-butoxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumaryl 7-amide + H2O - Sus scrofa ? - ? 74470 3.4.21.9 tert-butoxycarbonyl-Leu-Thr-Arg-4-methylcoumarin 7-amide + H2O weak activity Sus scrofa ? - ? 74472 3.4.21.9 tert-butoxycarbonyl-Phe-Ser-Arg-4-methylcoumarin 7-amide + H2O weak activity Sus scrofa ? - ? 74474 3.4.21.9 tert-butoxycarbonyl-Val-Leu-Lys-4-methylcoumarin 7-amide + H2O weak activity Sus scrofa ? - ? 74473 3.4.21.9 thiobenzyl benzyloxy-carbonyl-L-lysinate + H2O - Homo sapiens ? - ? 366197 3.4.21.9 thiobenzyl benzyloxy-carbonyl-L-lysinate + H2O - Homo sapiens 3-carboxy-4-nitrophenoxide + ? - ? 419144 3.4.21.9 thiobenzyl benzyloxy-carbonyl-L-lysinate + H2O - Bos taurus 3-carboxy-4-nitrophenoxide + ? - ? 419144 3.4.21.9 thiobenzyl benzyloxycarbonyl-L-lysinate + H2O - Bos taurus ? - ? 74466 3.4.21.9 thiobenzyl benzyloxycarbonyl-L-lysinate + H2O Z-Lys-SBzl Homo sapiens 3-carboxy-4-nitrothiophenoxide + ? - ? 394520 3.4.21.9 thioredoxin-DDDD156K-mouse NT-proCNP(2-40)-DDDK201SRLLR + H2O 22kDA substrate Sus scrofa cleavage products of thioredoxin-DDDD156K-mouse NT-proCNP(2-40)-DDDK201SRLLR 21 and 16 kDa cleavage products using the recombinant enzyme, multiple cleavage products using the native enzyme ? 394521 3.4.21.9 thioredoxin-DDDD156RRK-mouse NT-proCNP(2-40)-DDDK201SRLLR + H2O 22kDA substrate Sus scrofa cleavage products of thioredoxin-DDDD156RRK-mouse NT-proCNP(2-40)-DDDK201SRLLR 21 and 16 kDa cleavage products using the recombinant enzyme, multiple cleavage products using the native enzyme ? 394522 3.4.21.9 thioredoxin-DDDD156SDK-mouse NT-proCNP(2-40)-DDDK201SRLLR + H2O 22kDA substrate Sus scrofa cleavage products of thioredoxin-DDDD156SDK-mouse NT-proCNP(2-40)-DDDK201SRLLR 21 and 16 kDa cleavage products using the recombinant enzyme, multiple cleavage products using the native enzyme ? 394523 3.4.21.9 thioredoxin-DDDD156SK-mouse NT-proCNP(2-40)-DDDK201SRLLR + H2O 22kDA substrate, 9 fusion proteins are tested as substrates, addition of SRLLR residues leads to an increase in activity Sus scrofa cleavage products of thioredoxin-DDDD156SK-mouse NT-proCNP(2-40)-DDDK201SRLLR 21 and 16 kDa cleavage products using the recombinant enzyme, multiple cleavage products using the native enzyme ? 394524 3.4.21.9 thioredoxin-DDDD156SRK-mouse NT-proCNP(2-40)-DDDK201SRLLR + H2O 22kDA substrate Sus scrofa cleavage products of thioredoxin-DDDD156SRK-mouse NT-proCNP(2-40)-DDDK201SRLLR 21 and 16 kDa cleavage products using the recombinant enzyme, multiple cleavage products using the native enzyme ? 394525 3.4.21.9 thioredoxin-DDDD156SRLK-mouse NT-proCNP(2-40)-DDDK201SRLLR + H2O 22kDA substrate Sus scrofa cleavage products of thioredoxin-DDDD156SRLK-mouse NT-proCNP(2-40)-DDDK201SRLLR 21 and 16 kDa cleavage products using the recombinant enzyme, multiple cleavage products using the native enzyme ? 394526 3.4.21.9 thioredoxin-DDDD156SRLLK-mouse NT-proCNP(2-40)-DDDK201SRLLR + H2O 22kDA substrate Sus scrofa cleavage products of thioredoxin-DDDD156SRLLK-mouse NT-proCNP(2-40)-DDDK201SRLLR 21 and 16 kDa cleavage products using the recombinant enzyme, multiple cleavage products using the native enzyme ? 394527 3.4.21.9 thioredoxin-DDDD156SRLLRK-mouse NT-proCNP(2-40)-DDDK201SRLLR + H2O 22kDA substrate, best substrate for enterokinase activity Sus scrofa cleavage products of thioredoxin-DDDD156SRLLRK-mouse NT-proCNP(2-40)-DDDK201SRLLR 21 and 16 kDa cleavage products using the recombinant enzyme, multiple cleavage products using the native enzyme ? 394528 3.4.21.9 thioredoxin-DDDD156SSK-mouse NT-proCNP(2-40)-DDDK201SRLLR + H2O 22kDA substrate Sus scrofa cleavage products of thioredoxin-DDDD156SSK-mouse NT-proCNP(2-40)-DDDK201SRLLR 21 and 16 kDa cleavage products using the recombinant enzyme, multiple cleavage products using the native enzyme ? 394529 3.4.21.9 thioredoxin-fused N-terminal pro-C-type natriuretic peptide + H2O - Sus scrofa N-terminal pro-C-type natriuretic peptide fragment + ? - ? 385829 3.4.21.9 thioredoxin-human epidermal growth factor fusion protein + H2O Trx/hEGF Homo sapiens thioredoxin + human epidermal growth factor - ? 394530 3.4.21.9 thioredoxin-human epidermal growth factor fusion protein + H2O Trx/hEGF Bos taurus thioredoxin + human epidermal growth factor - ? 394530 3.4.21.9 thioredoxin-tumor necrosis factor-related apoptosis-inducing ligand fusion protein + H2O trx/TRAIL Homo sapiens thioredoxin + tumor necrosis factor-related apoptosis-inducing ligand enteropeptidase is used to cleave TRAIL from thioredoxin at enzyme-substrate molar ratio of 1 : 30,000 ? 394531 3.4.21.9 Tosyl-Arg methyl ester + H2O - Homo sapiens Tosyl-Arg + methanol - ? 15739 3.4.21.9 Tosyl-Arg methyl ester + H2O - Sus scrofa Tosyl-Arg + methanol - ? 15739 3.4.21.9 Tosyl-Arg methyl ester + H2O - Bos taurus Tosyl-Arg + methanol - ? 15739 3.4.21.9 tosyl-Lys methyl ester + H2O - Homo sapiens tosyl-Lys + methanol - ? 74462 3.4.21.9 tosyl-Lys methyl ester + H2O - Sus scrofa tosyl-Lys + methanol - ? 74462 3.4.21.9 tosyl-Lys methyl ester + H2O - Bos taurus tosyl-Lys + methanol - ? 74462 3.4.21.9 Trypsinogen + H2O - Homo sapiens ? - ? 16266 3.4.21.9 Trypsinogen + H2O - Sus scrofa ? - ? 16266 3.4.21.9 Trypsinogen + H2O - Bos taurus ? - ? 16266 3.4.21.9 Trypsinogen + H2O initiation of digestive enzyme activation by converting trypsinogen into trypsin Mus musculus ? - ? 16266 3.4.21.9 Trypsinogen + H2O initiates activation of pancreatic hydrolases by cleaving and activating trypsinogen Bos taurus ? - ? 16266 3.4.21.9 Trypsinogen + H2O enterokinase deficiency is a distinct clinical entity characterized by diarrhea, failure to thrive, hypoproteinemia, and edema. Acquired enterokinase deficiency may occur in some diffuse small bowel diseases. Steatorhea of cellac sprue may be due partly to the fact that deficiency of secretin and cholecystokinin may interfere with the action of enterokinase Homo sapiens ? - ? 16266 3.4.21.9 Trypsinogen + H2O the enzyme plays a key role in initiating the proteolytic digestion cascade in duodenum by converting trypsinogen to trypsin Bos taurus ? - ? 16266 3.4.21.9 Trypsinogen + H2O physiological activator of trypsinogen Homo sapiens ? - ? 16266 3.4.21.9 Trypsinogen + H2O physiological activator of trypsinogen Sus scrofa ? - ? 16266 3.4.21.9 Trypsinogen + H2O physiological activator of trypsinogen Bos taurus ? - ? 16266 3.4.21.9 Trypsinogen + H2O rapid activation of trypsinogen in pancreatic juice. The enzyme has a key permissive role in protein digestion and plays an essential part in the zymogen mechanism that protects the pancreas from self-destruction Homo sapiens ? - ? 16266 3.4.21.9 trypsinogen + H2O - Homo sapiens trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O - Sus scrofa trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O - Bos taurus trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O - Oryzias latipes trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O human cationic trypsinogen is activated much more readily than bovine trypsinogen Homo sapiens trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O specificity for the sequence (Asp)4-Lys-Ile Homo sapiens trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O specificity for the sequence (Asp)4-Lys-Ile Sus scrofa trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O specificity for the sequence (Asp)4-Lys-Ile Bos taurus trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O bovine trypsinogen Struthio camelus trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O cleaved exclusively at the Lys6-Ile7-peptide bond Bos taurus trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O the specificity site of enterokinase recognizes in trypsinogen not merely the basic residue of the -Lys6-/-Ile7-bond, which is split during activation of the zymogen but also recognizes the sequence -Asp4-Lys, residues 2 to 6, which is present in all of the trypsinogens so far studied Sus scrofa trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O cleavage after the sequence DDDDK Homo sapiens trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O cleavage after the sequence DDDDK Bos taurus trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O cleavage sequence DDDDK-X Homo sapiens trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O recognition sequence (Asp)4-Lys Homo sapiens trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O recognition site DDDDK Oryzias latipes trypsin + ? - ? 74459 3.4.21.9 trypsinogen + H2O - Homo sapiens trypsin - ? 394535 3.4.21.9 trypsinogen + H2O - Sus scrofa trypsin - ? 394535 3.4.21.9 trypsinogen + H2O - Bos taurus trypsin - ? 394535 3.4.21.9 trypsinogen + H2O activation by by endoproteolytic cleavage Homo sapiens trypsin - ? 394535 3.4.21.9 trypsinogen + H2O the enzyme cleaves after lysine residue if the Lys is preceded by four Asp and not followed by a Pro, and it shows high specificity of the target site. The light chain represents the catalytic enzyme subunit Homo sapiens trypsin - ? 394535 3.4.21.9 Val-(Ala)2-(Asp)2-Lys-Ile-Val-Gly + H2O 107% relative activity Sus scrofa Val-(Ala)2-(Asp)2-Lys + Ile-Val-Gly - ? 406849 3.4.21.9 Val-(Ala)2-(Asp)2-Lys-Ile-Val-Gly + H2O 109.3% relative activity Bos taurus Val-(Ala)2-(Asp)2-Lys + Ile-Val-Gly - ? 406849 3.4.21.9 Val-(Ala)2-Asp-Ala-Lys-Ile-Val-Gly + H2O 16.8% relative activity Bos taurus Val-(Ala)2-Asp-Ala-Lys + Ile-Val-Gly - ? 406850 3.4.21.9 Val-(Ala)2-Asp-Ala-Lys-Ile-Val-Gly + H2O 21.1% relative activity Sus scrofa Val-(Ala)2-Asp-Ala-Lys + Ile-Val-Gly - ? 406850 3.4.21.9 Val-(Ala)3-Asp-Lys-Ile-Val-Gly + H2O 75.6% relative activity Bos taurus Val-(Ala)3-Asp-Lys + Ile-Val-Gly - ? 406851 3.4.21.9 Val-(Ala)3-Asp-Lys-Ile-Val-Gly + H2O 81.2% relative activity Sus scrofa Val-(Ala)3-Asp-Lys + Ile-Val-Gly - ? 406851 3.4.21.9 Val-(Asp)2-Ala-Asp-Lys-Ile-Val-Gly + H2O 76.1% relative activity Bos taurus Val-(Asp)2-Ala-Asp-Lys + Ile-Val-Gly - ? 406853 3.4.21.9 Val-(Asp)2-Ala-Asp-Lys-Ile-Val-Gly + H2O 93.1% relative activity Sus scrofa Val-(Asp)2-Ala-Asp-Lys + Ile-Val-Gly - ? 406853 3.4.21.9 Val-(Asp)3-Ala-Lys-Ile-Val-Gly + H2O 16.3% relative activity Bos taurus Val-(Asp)3-Ala-Lys + Ile-Val-Gly - ? 406854 3.4.21.9 Val-(Asp)3-Ala-Lys-Ile-Val-Gly + H2O 20.2% relative activity Sus scrofa Val-(Asp)3-Ala-Lys + Ile-Val-Gly - ? 406854 3.4.21.9 Val-(Asp)3-Glu-Lys-Ile-Val-Gly + H2O 42.5% relative activity Bos taurus Val-(Asp)3-Glu-Lys + Ile-Val-Gly - ? 406855 3.4.21.9 Val-(Asp)3-Glu-Lys-Ile-Val-Gly + H2O 64.9% relative activity Sus scrofa Val-(Asp)3-Glu-Lys + Ile-Val-Gly - ? 406855 3.4.21.9 Val-(Asp)4-Arg-Ile-Val-Gly + H2O 115.2% relative activity Bos taurus Val-(Asp)4-Arg + Ile-Val-Gly - ? 406856 3.4.21.9 Val-(Asp)4-Arg-Ile-Val-Gly + H2O 128.2% relative activity Sus scrofa Val-(Asp)4-Arg + Ile-Val-Gly - ? 406856 3.4.21.9 Val-(Asp)4-Lys-Ile-Val-Gly + H2O 100% relative activity Bos taurus Val-(Asp)4-Lys + Ile-Val-Gly - ? 406857 3.4.21.9 Val-(Asp)4-Lys-Ile-Val-Gly + H2O 100% relative activity Sus scrofa Val-(Asp)4-Lys + Ile-Val-Gly - ? 406857 3.4.21.9 Val-Ala-(Asp)2-Ala-Lys-Ile-Val-Gly + H2O 4.3% relative activity Bos taurus Val-Ala-(Asp)2-Ala-Lys + Ile-Val-Gly - ? 406858 3.4.21.9 Val-Ala-(Asp)2-Ala-Lys-Ile-Val-Gly + H2O 5.6% relative activity Sus scrofa Val-Ala-(Asp)2-Ala-Lys + Ile-Val-Gly - ? 406858 3.4.21.9 Val-Ala-(Asp)3-Lys-Ile-Val-Gly + H2O 104.2% relative activity Bos taurus Val-Ala-(Asp)3-Lys + Ile-Val-Gly - ? 406859 3.4.21.9 Val-Ala-(Asp)3-Lys-Ile-Val-Gly + H2O 123.7% relative activity Sus scrofa Val-Ala-(Asp)3-Lys + Ile-Val-Gly - ? 406859 3.4.21.9 Val-Ala-Asp-Ala-Asp-Lys-Ile-Val-Gly + H2O 35.8% relative activity Sus scrofa Val-Ala-Asp-Ala-Asp-Lys + Ile-Val-Gly - ? 406860 3.4.21.9 Val-Ala-Asp-Ala-Asp-Lys-Ile-Val-Gly + H2O 48.1% relative activity Bos taurus Val-Ala-Asp-Ala-Asp-Lys + Ile-Val-Gly - ? 406860 3.4.21.9 Val-Asp-(Ala)2-Asp-Lys-Ile-Val-Gly + H2O 82.6% relative activity Bos taurus Val-Asp-(Ala)2-Asp-Lys + Ile-Val-Gly - ? 406861 3.4.21.9 Val-Asp-(Ala)2-Asp-Lys-Ile-Val-Gly + H2O 95.8% relative activity Sus scrofa Val-Asp-(Ala)2-Asp-Lys + Ile-Val-Gly - ? 406861 3.4.21.9 Val-Asp-(Ala)3-Lys-Ile-Val-Gly + H2O 6.6% relative activity Sus scrofa Val-Asp-(Ala)3-Lys + Ile-Val-Gly - ? 406862 3.4.21.9 Val-Asp-(Ala)3-Lys-Ile-Val-Gly + H2O 7.1% relative activity Bos taurus Val-Asp-(Ala)3-Lys + Ile-Val-Gly - ? 406862 3.4.21.9 Val-Asp-Ala-(Asp)2-Lys-Ile-Val-Gly + H2O 117.4% relative activity Bos taurus Val-Asp-Ala-(Asp)2-Lys + Ile-Val-Gly - ? 406852 3.4.21.9 Val-Asp-Ala-(Asp)2-Lys-Ile-Val-Gly + H2O 119.7% relative activity Sus scrofa Val-Asp-Ala-(Asp)2-Lys + Ile-Val-Gly - ? 406852 3.4.21.9 Val-Asp-Ala-Asp-Ala-Lys-Ile-Val-Gly + H2O 21.1% relative activity Bos taurus Val-Asp-Ala-Asp-Ala-Lys + Ile-Val-Gly - ? 406863 3.4.21.9 Val-Asp-Ala-Asp-Ala-Lys-Ile-Val-Gly + H2O 22.5% relative activity Sus scrofa Val-Asp-Ala-Asp-Ala-Lys + Ile-Val-Gly - ? 406863 3.4.21.9 Val-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O - Bos taurus ? - ? 74457 3.4.21.9 VDYRFL-SA-PE + H2O peptide conjugated to fluorescent probe streptavidin-conjugated phycoerythrin Homo sapiens VDYRFL + SA-PE - ? 394540 3.4.21.9 VLDRWM-SA-PE + H2O peptide conjugated to fluorescent probe streptavidin-conjugated phycoerythrin Homo sapiens VLDRWM + SA-PE - ? 394542 3.4.21.9 VRDYRM-SA-PE + H2O peptide conjugated to fluorescent probe streptavidin-conjugated phycoerythrin Homo sapiens VRDYRM + SA-PE - ? 394543 3.4.21.9 WDDKG + H2O - Bos taurus ? - ? 385923 3.4.21.9 WDDRG + H2O - Bos taurus ? - ? 385924 3.4.21.9 Z-Ala-Ala-Arg-p-nitroanilide + H2O - Homo sapiens p-nitroaniline + Z-Ala-Ala-Arg - ? 394562 3.4.21.9 Z-Ala-Leu-Arg-p-nitroanilide + H2O - Homo sapiens p-nitroaniline + Z-Ala-Leu-Arg - ? 394564 3.4.21.9 Z-Ala-Met-Arg-p-nitroanilide + H2O - Homo sapiens p-nitroaniline + Z-Ala-Met-Arg - ? 394565 3.4.21.9 Z-Ala-Phe-Arg-p-nitroanilide + H2O - Homo sapiens p-nitroaniline + Z-Ala-Phe-Arg - ? 394566 3.4.21.9 Z-Ala-Trp-Arg-p-nitroanilide + H2O - Homo sapiens Z-Ala-Trp-Arg + 4-nitroaniline - ? 394567 3.4.21.9 Z-Ala-Tyr-Arg-p-nitroanilide + H2O - Homo sapiens p-nitroaniline + Z-Ala-Tyr-Arg - ? 394568 3.4.21.9 Z-Gly-Pro-Arg-p-nitroanilide + H2O - Homo sapiens p-nitroaniline + Z-Gly-Pro-Arg - ? 394574 3.4.21.9 Z-Lys-SBzl + H2O - Homo sapiens ? - ? 432503 3.4.21.9 Z-Phe-Arg-4-methylcoumaryl-7-amide + H2O - Oryzias latipes 7-amino-4-methylcoumarin + Z-Phe-Arg - ? 394575 3.4.21.9 Z-Pro-Phe-Arg-p-nitroanilide + H2O - Homo sapiens p-nitroaniline + Z-Pro-Phe-Arg - ? 394576