3.4.21.82 2-Aminobenzoyl-Ala-Ala-Glu-Val-Tyr(NO2)-Asp-OH + H2O - Streptomyces griseus 2-Aminobenzoyl-Ala-Ala-Glu + Val-Tyr(NO2)-Asp-OH - ? 15722 3.4.21.82 azocasein + H2O - Streptomyces griseus ? - ? 15347 3.4.21.82 benzlyoxycarbonyl-Ala-Ala-Leu-Glu-p-nitroanilide + H2O - Bacillus intermedius ? - ? 365996 3.4.21.82 benzlyoxycarbonyl-Ala-Ala-Met-Glu-p-nitroanilide + H2O - Bacillus intermedius ? - ? 365993 3.4.21.82 benzlyoxycarbonyl-Ala-Ala-Met-Glu-p-nitroanilide + H2O - Bacillus intermedius Mrz 19 ? - ? 365993 3.4.21.82 benzlyoxycarbonyl-Ala-Ala-Phe-Glu-p-nitroanilide + H2O - Bacillus intermedius ? - ? 365995 3.4.21.82 benzlyoxycarbonyl-Ala-Ala-Trp-Glu-p-nitroanilide + H2O - Bacillus intermedius ? - ? 365994 3.4.21.82 benzlyoxycarbonyl-Ala-Ala-Trp-Glu-p-nitroanilide + H2O - Bacillus intermedius Mrz 19 ? - ? 365994 3.4.21.82 benzlyoxycarbonyl-Glu-p-nitroanilide + H2O - Bacillus intermedius ? - ? 365997 3.4.21.82 benzlyoxycarbonyl-Gly-Ala-Ala-Glu-p-nitroanilide + H2O - Bacillus intermedius ? - ? 365998 3.4.21.82 benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Gly-Leu-NH2 - Bacillus licheniformis ? - ? 365978 3.4.21.82 benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Ile-NH2 - Bacillus licheniformis ? - ? 365973 3.4.21.82 benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Leu-Ala-NH2 - Bacillus licheniformis ? - ? 365976 3.4.21.82 benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Leu-GLy-NH2 - Bacillus licheniformis ? - ? 365977 3.4.21.82 benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Leu-NH2 - Bacillus licheniformis ? - ? 365972 3.4.21.82 benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Met-NH2 - Bacillus licheniformis ? - ? 365975 3.4.21.82 benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Nva-NH2 - Bacillus licheniformis ? - ? 365974 3.4.21.82 benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Gly-Leu-NH2 - Bacillus licheniformis ? - ? 365985 3.4.21.82 benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Ile-NH2 - Bacillus licheniformis ? - ? 365980 3.4.21.82 benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Leu-Ala-NH2 - Bacillus licheniformis ? - ? 365983 3.4.21.82 benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Leu-Gly-NH2 - Bacillus licheniformis ? - ? 365984 3.4.21.82 benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Leu-NH2 - Bacillus licheniformis ? - ? 365979 3.4.21.82 benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Met-NH2 - Bacillus licheniformis ? - ? 365982 3.4.21.82 benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Nva-NH2 - Bacillus licheniformis ? - ? 365981 3.4.21.82 benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Gly-Leu-NH2 - Bacillus licheniformis ? - ? 365992 3.4.21.82 benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Ile-NH2 - Bacillus licheniformis ? - ? 365987 3.4.21.82 benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Leu-Ala-NH2 - Bacillus licheniformis ? - ? 365990 3.4.21.82 benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Leu-Gly-NH2 - Bacillus licheniformis ? - ? 365991 3.4.21.82 benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Leu-NH2 - Bacillus licheniformis ? - ? 365986 3.4.21.82 benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Met-NH2 - Bacillus licheniformis ? - ? 365989 3.4.21.82 benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Nva-NH2 - Bacillus licheniformis ? - ? 365988 3.4.21.82 benzyloxycarbonyl-Ala-carboxyethyl thioester + Gly-Leu-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365971 3.4.21.82 benzyloxycarbonyl-Ala-carboxyethyl thioester + Ile-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365966 3.4.21.82 benzyloxycarbonyl-Ala-carboxyethyl thioester + Leu-Ala-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365969 3.4.21.82 benzyloxycarbonyl-Ala-carboxyethyl thioester + Leu-Gly-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365970 3.4.21.82 benzyloxycarbonyl-Ala-carboxyethyl thioester + Leu-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365965 3.4.21.82 benzyloxycarbonyl-Ala-carboxyethyl thioester + Met-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365968 3.4.21.82 benzyloxycarbonyl-Ala-carboxyethyl thioester + Nva-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365967 3.4.21.82 benzyloxycarbonyl-Ala-carboxymethyl thioester + Gly-Leu-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365964 3.4.21.82 benzyloxycarbonyl-Ala-carboxymethyl thioester + Ile-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365959 3.4.21.82 benzyloxycarbonyl-Ala-carboxymethyl thioester + Leu-Ala-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365962 3.4.21.82 benzyloxycarbonyl-Ala-carboxymethyl thioester + Leu-Gly-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365963 3.4.21.82 benzyloxycarbonyl-Ala-carboxymethyl thioester + Leu-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365958 3.4.21.82 benzyloxycarbonyl-Ala-carboxymethyl thioester + Met-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365961 3.4.21.82 benzyloxycarbonyl-Ala-carboxymethyl thioester + Nva-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365960 3.4.21.82 benzyloxycarbonyl-Asp-methyl ester + Gly-Leu-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365957 3.4.21.82 benzyloxycarbonyl-Asp-methyl ester + Ile-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365952 3.4.21.82 benzyloxycarbonyl-Asp-methyl ester + Leu-Ala-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365955 3.4.21.82 benzyloxycarbonyl-Asp-methyl ester + Leu-Gly-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365956 3.4.21.82 benzyloxycarbonyl-Asp-methyl ester + Leu-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365951 3.4.21.82 benzyloxycarbonyl-Asp-methyl ester + Met-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365954 3.4.21.82 benzyloxycarbonyl-Asp-methyl ester + Nva-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365953 3.4.21.82 benzyloxycarbonyl-Glu-methyl ester + Gly-Leu-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365943 3.4.21.82 benzyloxycarbonyl-Glu-methyl ester + Ile-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365938 3.4.21.82 benzyloxycarbonyl-Glu-methyl ester + Leu-Ala-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365941 3.4.21.82 benzyloxycarbonyl-Glu-methyl ester + Leu-Gly-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365942 3.4.21.82 benzyloxycarbonyl-Glu-methyl ester + Leu-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365937 3.4.21.82 benzyloxycarbonyl-Glu-methyl ester + Met-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365940 3.4.21.82 benzyloxycarbonyl-Glu-methyl ester + Nva-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365939 3.4.21.82 benzyloxycarbonyl-Glu-methyl thioester + Gly-Leu-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365950 3.4.21.82 benzyloxycarbonyl-Glu-methyl thioester + Ile-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365945 3.4.21.82 benzyloxycarbonyl-Glu-methyl thioester + Leu-Ala-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365948 3.4.21.82 benzyloxycarbonyl-Glu-methyl thioester + Leu-Gly-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365949 3.4.21.82 benzyloxycarbonyl-Glu-methyl thioester + Leu-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365944 3.4.21.82 benzyloxycarbonyl-Glu-methyl thioester + Met-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365947 3.4.21.82 benzyloxycarbonyl-Glu-methyl thioester + Nva-NH2 protease catalyzed acyltransfer from substrates to amino acid amides Bacillus licheniformis ? - ? 365946 3.4.21.82 Benzyloxycarbonyl-Leu-Leu-Glu 2-naphthylamide + H2O - Streptomyces griseus Benzyloxycarbonyl-Leu-Leu-Glu + 2-naphthylamine - ? 15723 3.4.21.82 Benzyloxycarbonyl-Phe-Leu-Glu 2-nitroanilide + H2O - Streptomyces griseus Benzyloxycarbonyl-Phe-Leu-Glu + 4-nitroaniline - ? 15724 3.4.21.82 Bovine serum albumin + H2O BSA Streptomyces griseus ? - ? 16295 3.4.21.82 casein + H2O - Bacillus intermedius hydrolyzed casein - ? 15305 3.4.21.82 casein + H2O - Bacillus intermedius Mrz 19 hydrolyzed casein - ? 15305 3.4.21.82 insulin + H2O hydrolyzes Glu4-Gln5, Glu17-Asp18, and Cys11-Ser12 bonds in the oxidized A-chain of insulin and Glu13-Ala14, Glu21-Arg22, Cys7-Gly8, and Cys19-Gly20 bonds in the oxidized B-chain of insulin Bacillus intermedius ? - ? 36836 3.4.21.82 insulin + H2O hydrolyzes Glu4-Gln5, Glu17-Asp18, and Cys11-Ser12 bonds in the oxidized A-chain of insulin and Glu13-Ala14, Glu21-Arg22, Cys7-Gly8, and Cys19-Gly20 bonds in the oxidized B-chain of insulin Bacillus intermedius Mrz 19 ? - ? 36836 3.4.21.82 additional information narrow substrate preference Streptomyces griseus ? - ? 89 3.4.21.82 additional information preferentially hydrolyzes peptide bonds on the carbonyl-terminal side of either glutamic acid or aspartic acid Streptomyces griseus ? - ? 89 3.4.21.82 additional information preference for aspartic acid at P4, preference for Ala and Val at P3, preference for Pro and Val at P2, proline is disfavored at P3, P1', and P2' and aspartic acid is disfavored at P1' Streptomyces griseus ? - ? 89 3.4.21.82 additional information the enzyme favors Pro and Leu residues at S2, while S3 subsite prefers Phe over either Ala or Leu Streptomyces griseus ? - ? 89 3.4.21.82 additional information hydrolyzes Glu-Xaa bonds approximately 100-fold faster than Asp-Xaa bonds Streptomyces griseus ? - ? 89 3.4.21.82 additional information binding site comprises at least 6 subsites Streptomyces griseus ? - ? 89 3.4.21.82 additional information pronounced preference for Glu-Xaa bonds versus Asp-Xaa bonds Streptomyces griseus ? - ? 89 3.4.21.82 additional information does not hydrolyze the subtilisin substrate benzyloxycarbonyl-Ala-Ala-Leu-p-nitroanilide, only little activity with benzyloxycarbonyl-Ala-Ala-Trp-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Leu-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Phe-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Met-Asp-p-nitroanilide, and benzyloxycarbonyl-Gly-Ala-Ala-Asp-p-nitroanilide Bacillus intermedius ? - ? 89 3.4.21.82 additional information does not hydrolyze the subtilisin substrate benzyloxycarbonyl-Ala-Ala-Leu-p-nitroanilide, only little activity with benzyloxycarbonyl-Ala-Ala-Trp-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Leu-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Phe-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Met-Asp-p-nitroanilide, and benzyloxycarbonyl-Gly-Ala-Ala-Asp-p-nitroanilide Bacillus intermedius Mrz 19 ? - ? 89 3.4.21.82 S-AAPEpNA + H2O specific SGPE substrate Streptomyces griseus ? - ? 394494 3.4.21.82 Synthetic peptide substrates containing an N-terminal anthraniloyl group and a 3-nitrotyrosine close to the C-terminus + H2O - Streptomyces griseus ? - ? 15721 3.4.21.82 tert-butoxycarbonyl-Ala-Ala-Pro-Asp 4-nitroanilide + H2O slowly Streptomyces griseus tert-butoxycarbonyl-Ala-Ala-Pro-Asp + 4-nitroaniline - ? 15712 3.4.21.82 tert-butoxycarbonyl-Ala-Ala-Pro-Glu 4-nitroanilide + H2O - Streptomyces griseus tert-butoxycarbonyl-Ala-Ala-Pro-Glu + 4-nitroaniline - ? 15711 3.4.21.82 tert-butoxycarbonyl-Ala-Phe-Pro-Glu 4-nitroanilide + H2O - Streptomyces griseus tert-butoxycarbonyl-Ala-Phe-Pro-Glu + 4-nitroaniline - ? 15713 3.4.21.82 Tert-Butyloxycarbonyl-Ala-Ala-Ala-Glu 4-nitroanilide + H2O - Streptomyces griseus Tert-Butyloxycarbonyl-Ala-Ala-Ala-Glu + 4-nitroaniline - ? 15715 3.4.21.82 Tert-Butyloxycarbonyl-Ala-Ala-Gly-Glu 4-nitroanilide + H2O - Streptomyces griseus Tert-Butyloxycarbonyl-Ala-Ala-Gly-Glu + 4-nitroaniline - ? 15714 3.4.21.82 tert-butyloxycarbonyl-Ala-Ala-Leu-Glu-4-nitroanilide + H2O - Streptomyces griseus tert-butyloxycarbonyl-Ala-Ala-Leu-Glu + 4-nitroaniline - ? 453051 3.4.21.82 Tert-Butyloxycarbonyl-Ala-Ala-Phe-Glu 4-nitroanilide + H2O - Streptomyces griseus Tert-Butyloxycarbonyl-Ala-Ala-Phe-Glu + 4-nitroaniline - ? 15716 3.4.21.82 Tert-Butyloxycarbonyl-Ala-Ala-Pro-Glu 4-nitroanilide + H2O - Streptomyces griseus Tert-Butyloxycarbonyl-Ala-Ala-Pro-Glu + 4-nitroaniline - ? 15717 3.4.21.82 Tert-Butyloxycarbonyl-Ala-Leu-Pro-Glu 4-nitroanilide + H2O - Streptomyces griseus Tert-Butyloxycarbonyl-Ala-Leu-Pro-Glu + 4-nitroaniline - ? 15718 3.4.21.82 Tert-Butyloxycarbonyl-Ala-Phe-Pro-Glu 4-nitroanilide + H2O - Streptomyces griseus Tert-Butyloxycarbonyl-Ala-Phe-Pro-Glu + 4-nitroaniline - ? 15719 3.4.21.82 Tert-Butyloxycarbonyl-Ala-Pro-Glu 4-nitroanilide + H2O - Streptomyces griseus Tert-Butyloxycarbonyl-Ala-Pro-Glu + 4-nitroaniline - ? 15720