3.4.21.47 C2 fragment + H2O C-terminal fragment originating from the processing of meningococcal proteases Homo sapiens ? - ? 448487 3.4.21.47 complement component C3 + H2O - Homo sapiens complement component C3a + complement component C3b - ? 36853 3.4.21.47 complement component C3 + H2O - Rattus norvegicus complement component C3a + complement component C3b - ? 36853 3.4.21.47 complement component C3 + H2O - Mus musculus complement component C3a + complement component C3b - ? 36853 3.4.21.47 complement component C3 + H2O hydrolysis of peptide bond 77 Arg-Ser of the a-chain Homo sapiens complement component C3a + complement component C3b - ? 36853 3.4.21.47 complement component C3 + H2O complement component C3 is the preferred substrate. Cleavage of the preferred C3 substrate and deposition of C3b effectively switches the output of the enzyme from C3b to C5b, resulting in initiation of the cytosolic process of complement Homo sapiens complement component C3a + complement component C3b - ? 36853 3.4.21.47 complement component C3 + H2O analysis of structure and function of complement component C3b in complex with the macrophage-expressed complement receptor CRIg, CRIg shown as an inhibitor of alternative complement convertases Homo sapiens complement component C3a + complement component C3b - ? 36853 3.4.21.47 complement component C3 + H2O cleavage of complement component C3 mediated through the MBL pathway in human serum analyzed, mannan-binding lectin (MBL) promotes activation of C3, combined action of MBL-associated serine proteases MASP-1 and MASP-2 required, involvement of the alternative pathway not detected Homo sapiens complement component C3a + complement component C3b - ? 36853 3.4.21.47 complement component C3 + H2O cleavage of complement component C3 through the mannan-binding lectin pathway in mouse sera determined Mus musculus complement component C3a + complement component C3b - ? 36853 3.4.21.47 complement component C3 + H2O complement activation in properdin-deficient mice analyzed Mus musculus complement component C3a + complement component C3b - ? 36853 3.4.21.47 complement component C3 + H2O interaction studies with decay-accelerating factor DAF mediating decay of the alternative pathway C3 convertase, C3bBb, but not of the inactive proenzyme C3bB Homo sapiens complement component C3a + complement component C3b - ? 36853 3.4.21.47 complement component C3 + H2O potencies of the amplification loop of the alternative complement pathway summarized Homo sapiens complement component C3a + complement component C3b - ? 36853 3.4.21.47 complement component C3 + H2O properdin-deficient patients susceptible to lethal meningococcal infection, studies on yet unknown mechanism of selective predisposition Homo sapiens complement component C3a + complement component C3b - ? 36853 3.4.21.47 complement component C3 + H2O studies on fluid-phase formation of initial convertase by fluorescence resonance energy transfer (FRET) Homo sapiens complement component C3a + complement component C3b - ? 36853 3.4.21.47 complement component C3 zymogen + H2O - Homo sapiens complement component C3b + complement component C3a - ? 430939 3.4.21.47 complement component C3 zymogen + H2O activation Homo sapiens complement component C3b + complement component C3a - ? 430939 3.4.21.47 complement component C5 + H2O - Homo sapiens complement component C5a + complement component C5b - ? 36902 3.4.21.47 complement component C5 + H2O - Mus musculus complement component C5a + complement component C5b - ? 36902 3.4.21.47 complement component C5 + H2O methionine-modified enzyme has significantly lower C5-cleavage ability than that of unmodified enzyme Homo sapiens complement component C5a + complement component C5b - ? 36902 3.4.21.47 complement component C5 zymogen + H2O - Homo sapiens complement component C5b + complement component C5a - ? 430942 3.4.21.47 complement component C5 zymogen + H2O activation Homo sapiens complement component C5b + complement component C5a - ? 430942 3.4.21.47 additional information fragment Bb is the catalytic subunit of the enzyme, isolated Bb is unable to cleave either C3 or C5 Homo sapiens ? - ? 89 3.4.21.47 additional information the enzyme is involved in the alternative pathway of human complement Homo sapiens ? - ? 89 3.4.21.47 additional information the activation peptide, C5a, possesses potent spasmogenic and chemotactic activity Homo sapiens ? - ? 89 3.4.21.47 additional information the enzyme has a very short half-life. Dissociation of the two noncovalently bound subunits proceeds with a half-life of 1-3 min at 37°C under physiological conditions, and this rate increases greatly if regulatory proteins are present. Numerous decay-accelerating proteins are present in plasma and on host cells that bind to the noncatalytic subunit C3b and increase the rate at which the catalytic subunit Bb is released into the medium. Bb loses its enzymatic activity and its ability to bind to C3b upon release. Although C3b is able to rebind Bb and reform the enzyme, the interaction with most decay-accelerating factors also leads to permanent proteolytic interaction of the cell-bound subunit C3b by a fluid-phase protease Factor I. These regulatory events limit cleavage of C3, reduce release of the anaphylatoxin C3a and control the formation of more efficient C5 convertase enzymes Homo sapiens ? - ? 89 3.4.21.47 additional information early complement stimulation in trauma patients analyzed, correlation with injury severity, tissue hypoperfusion and worse clinical outcomes identified Homo sapiens ? - ? 89 3.4.21.47 additional information C3b binds uniformly to substances on the surface of Escherichia coli K12, whereas it shows a bimodal distribution on the surface of Staphylococcus aureus P209. Autoactivation activity of C3 Tachypleus tridentatus ? - ? 89 3.4.21.47 neisserial heparin binding antigen + H2O also known as GNA2132 (genome-derived Neisseria antigen 2132) Homo sapiens ? - ? 448808 3.4.21.47 t-butyloxycarbonyl-Gly-L-Leu-L-Ala-L-Arg-thiobenzyl ester substrate of enzyme subunit Bb Homo sapiens ? - ? 385790 3.4.21.47 tert-butoxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens tert-butoxycarbonyl-Leu-Gly-Arg + 7-amino-4-methylcoumarin - ? 36904 3.4.21.47 tert-butoxycarbonyl-norleucine-Gln-Leu-Gly-Arg-7-amido-4-methylcoumarin + H2O - Homo sapiens tert-butoxycarbonyl-norleucine-Gln-Leu-Gly-Arg + 7-amino-4-methylcoumarin - ? 36903