3.4.21.41 acetyl-Arg methyl ester + H2O - Homo sapiens acetyl-Arg + methanol - ? 65161 3.4.21.41 acetyl-Gly-Lys-methyl ester + H2O - Homo sapiens ? - ? 365421 3.4.21.41 benzyloxycarbonyl-Gly-Arg-thiobenzyl ester + H2O - Homo sapiens ? - ? 365417 3.4.21.41 benzyloxycarbonyl-Lys-thiobenzyl ester + H2O - Homo sapiens benzyloxycarbonyl-Lys + phenylmethanethiol - ? 15670 3.4.21.41 chordin + H2O C-terminal domains CUB1 and CUB2 are required for the ventralizing activity of Xld and for its ability to cleave chordin Xenopus laevis ? - ? 384449 3.4.21.41 complement C1q zymogen + H2O - Homo sapiens active complement C1q + ? - ? 430932 3.4.21.41 complement C1q zymogen + H2O no or reduced activity with substrate mutants K59A, K61A, K58A, and K58A/K59A/K61A Homo sapiens active complement C1q + ? - ? 430932 3.4.21.41 complement component C1s - Homo sapiens ? - ? 369674 3.4.21.41 complement component C1s binding of C1 to activator is mediated by C1q and triggers activation of proenzyme C1r into an active protease C1rbar, which in turn activates C1s, thereby initiating the classical pathway of complement Homo sapiens ? - ? 369674 3.4.21.41 complement component C1s regulation of the synthesis Homo sapiens ? - ? 369674 3.4.21.41 complement component C1s activates the proenzyme form of C1s by limited proteolysis Homo sapiens ? - ? 369674 3.4.21.41 complement component C1s + H2O - Homo sapiens complement component C1sbar - ? 65157 3.4.21.41 complement component C1s + H2O cleavage of a single Arg-Ile or Lys-Ile bond in C1s Homo sapiens complement component C1sbar - ? 65157 3.4.21.41 complement component C1s + H2O cleavage of a single Arg-Ile bond in the sequence Lys-Gln-Arg-Ile-Ile-Gly Homo sapiens complement component C1sbar - ? 65157 3.4.21.41 complement component C1s + H2O C1rbar does not hydrolyze any amino acid ester tested nor any protein substrate except subcomponent C1s Homo sapiens complement component C1sbar - ? 65157 3.4.21.41 complement component C1s + H2O C1R and its activated fragments all cleave C1s, in the order of increasing efficiency: C21r, CCP1/2-SP, CCP2-SP. CCP1 is not involved in C1s recognition Homo sapiens complement component C1sbar - ? 65157 3.4.21.41 complement component C1s + H2O - Homo sapiens activated complement component C1s + ? - ? 393959 3.4.21.41 complement component C1s + H2O - Sebastes schlegelii activated complement component C1s + ? - ? 393959 3.4.21.41 complement component C1s + H2O - Oplegnathus fasciatus activated complement component C1s + ? - ? 393959 3.4.21.41 complement component C1s + H2O the enzyme is part of the Ca2+-dependent tetramer C1s-C1r-C1r-C1s, termed as C1 complex, which is associated with the recognition molecule C1q, autoactivation of C1r, which then activates proenzyme C1s through cleavage at an Arg-Ile bond in the serine domain, C1r is active in the classical pathway of the complement system Homo sapiens activated complement component C1s + ? - ? 393959 3.4.21.41 complement component C1s + H2O the enzyme is part of the Ca2+-dependent tetramer C1s-C1r-C1r-C1s, termed as C1 complex, which is associated with the recognition molecule C1q, autoactivation of C1r, which then activates proenzyme C1s through cleavage at an Arg-Ile bond in the serine domain, C1r is active in the classical pathway of the complement system, interaction anaylsis of the C1 complex and regulatory proteins, overview Homo sapiens activated complement component C1s + ? - ? 393959 3.4.21.41 complement component C1s + H2O C1r cleaves proenzyme C1s at an Arg-Ile bond in the serine domain Homo sapiens activated complement component C1s + ? - ? 393959 3.4.21.41 complement component C1s + H2O C1r cleaves proenzyme C1s at an Arg-Ile bond in the serine domain, enzyme-product binding structure, overview Homo sapiens activated complement component C1s + ? - ? 393959 3.4.21.41 MASP-3 K448Q zymogen + H2O poor activity with the wild-type MASP-3 Homo sapiens active MASP-3 protein + ? - ? 432111 3.4.21.41 additional information the ability of autoactivation of C1r and C1s cleavage activity is an inherent property of the SP domain Homo sapiens ? - ? 89 3.4.21.41 additional information the enzyme recognizes the a short collagen-like peptide containing the sequence Hyp-Gly-Lys-Leu-Gly-Pro Homo sapiens ? - ? 89 3.4.21.41 additional information the residues found in the activation loop of the zymogens capable of being activated by enzyme C1r play a major role in recognition of the active site of enzyme C1r Homo sapiens ? - ? 89 3.4.21.41 N-acetyl-Arg methyl ester + H2O - Homo sapiens N-acetyl-Arg + methanol - ? 454007 3.4.21.41 N-acetyl-Gly-Lys methyl ester + H2O - Homo sapiens N-acetyl-Gly-Lys + methanol - ? 65158 3.4.21.41 N-benzyloxycarbonyl-Gly-Arg thiobenzyl ester + H2O - Homo sapiens ? - ? 65156 3.4.21.41 N-carbobenzoxy-Lys-p-nitrophenyl ester + H2O - Homo sapiens N-carbobenzoxy-Lys + 4-nitrophenyl - ? 65160 3.4.21.41 N-carbobenzoxy-Tyr-p-nitrophenyl ester + H2O - Homo sapiens N-carbobenzoxy-Tyr + 4-nitrophenol - ? 65159 3.4.21.41 prohaptoglobin + H2O the enzyme cleaves prohaptoglobin after arginine R102 in variants Hp1F and Hp1S or after R161 in variant Hp2FS Homo sapiens haptoglobin + ? - ? 448876 3.4.21.41 succinylated casein + H2O - Sebastes schlegelii ? - ? 450698 3.4.21.41 zymogen C1s + H2O - Homo sapiens active C1s protease + ? - ? 431242 3.4.21.41 zymogen C1s + H2O the enzyme is active with the substrate fragment consisting of complement control protein domains, CCP1 and CCP2, plus serine protease domain of wild-type and mutant Q462N, Q462G, I464A, and Q462N/I464A forms of C1s, mutant Q462N/I464A substrate gives very low activity Homo sapiens active C1s protease + ? - ? 431242 3.4.21.41 zymogen C1s + H2O - Homo sapiens active protease C1s + ? - ? 431243