3.4.21.103 azocasein + H2O - Physarum flavicomum ? - ? 15347 3.4.21.103 bovine albumin + H2O - Dictyostelium discoideum ? - ? 362010 3.4.21.103 casein + H2O - Dictyostelium discoideum ? - ? 15445 3.4.21.103 Cytochrome c + H2O - Dictyostelium discoideum ? - ? 16271 3.4.21.103 Glucagon + H2O - Physarum polycephalum ? - ? 15578 3.4.21.103 Hemoglobin + H2O - Physarum polycephalum ? - ? 15450 3.4.21.103 Hemoglobin + H2O - Dictyostelium discoideum Hydrolyzed hemoglobin - ? 15477 3.4.21.103 Hemoglobin + H2O - Physarum polycephalum Hydrolyzed hemoglobin - ? 15477 3.4.21.103 Hemoglobin + H2O - Physarum flavicomum Hydrolyzed hemoglobin - ? 15477 3.4.21.103 hide powder azure + H2O - Dictyostelium discoideum ? - ? 15924 3.4.21.103 histone + H2O - Dictyostelium discoideum ? - ? 73504 3.4.21.103 insulin B chain + H2O - Physarum polycephalum ? - ? 37184 3.4.21.103 KPIEFF(NO2)RL + H2O - Physarum polycephalum ? - ? 365148 3.4.21.103 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O - Physarum polycephalum Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu - ? 16473 3.4.21.103 Lys-Pro-Ile-Glu-Phe-(4-nitrophenyl)Arg-Leu + H2O - Physarum polycephalum Lys-Pro-Ile-Glu-Phe + (4-nitrophenyl)Arg-Leu - ? 382225 3.4.21.103 additional information activates trypsinogen Dictyostelium discoideum ? - ? 89 3.4.21.103 additional information derivatives of basic amino acids and related peptides (proteinase E) Physarum polycephalum ? - ? 89 3.4.21.103 additional information not: N-acetyl-N-phenylalanyl-3,5-diiodo-L-tyrosine Physarum polycephalum ? - ? 89 3.4.21.103 additional information no hydrolysis of hemoglobin, casein, azocoll and Ala-Ala-Phe-7-amido-4-methylcoumarin Physarum polycephalum ? - ? 89 3.4.21.103 additional information involved in general protein degradation within the lysosomes of Dictyostelium discoideum Dictyostelium discoideum ? - ? 89 3.4.21.103 additional information in the combinatorial chromogenic peptide Lys-Pro-X-Glu-P1*Nph-X-Leu, the enzyme prefers specifically Nle (100%), Asp (86%), Leu (84%), Glu (78%), and Ala (66%) in decreasing order of relative activity at the P1 position and the rate varies over 100fold between the most preferred Nle (100%) and the least preferred Pro (0.7%) Physarum polycephalum ? - ? 89 3.4.21.103 additional information small hydrophobic residues are preferred at the P1' position, Ile for the P3 position and/or Gln in the P2' position are not optimal for the enzyme Physarum polycephalum ? - ? 89 3.4.21.103 ovalbumin + H2O - Dictyostelium discoideum ? - ? 37064 3.4.21.103 oxidized insulin B + H2O - Physarum polycephalum ? - ? 95034 3.4.21.103 oxidized insulin B chain + H2O the major cleavage sites are Gly8-Ser9, Leu11-Val12, Cys19-Gly20, Gly20-Glu21, and Phe24-Phe25 Physarum polycephalum ? - ? 37092 3.4.21.103 Oxidized insulin B-chain + H2O major cleavage sites: Gly8-Ser9 (most susceptible), Leu11-Val12, Cysteic acid19-Gly20 and Phe24-Phe25 Physarum polycephalum Hydrolyzed insulin B-chain - ? 15315 3.4.21.103 Substance P + H2O - Physarum polycephalum ? - ? 17205