3.4.14.2 (Ala)3 + H2O - Cavia porcellus ? - ? 73966 3.4.14.2 (Ala)3 + H2O - Rattus norvegicus ? - ? 73966 3.4.14.2 (Ala)3 + H2O - Sus scrofa ? - ? 73966 3.4.14.2 (Ala)3 + H2O L-Ala3 Bos taurus ? - ? 73966 3.4.14.2 (Ala)4 + H2O - Rattus norvegicus ? - ? 73967 3.4.14.2 (Gly-Pro-Ala)n + H2O - Sus scrofa Gly-Pro + Ala - ? 73981 3.4.14.2 Ala-Ala-2-naphthylamide + H2O - Rattus norvegicus Ala-Ala + 2-naphthylamine - ? 36470 3.4.14.2 Ala-Ala-2-naphthylamide + H2O - Bos taurus Ala-Ala + 2-naphthylamine - ? 36470 3.4.14.2 Ala-Ala-2-nitroanilide + H2O low activity Rattus norvegicus Ala-Ala + 2-nitroaniline - ? 364867 3.4.14.2 Ala-Ala-4-nitroanilide + H2O - Homo sapiens Ala-Ala + 4-nitroaniline - ? 36592 3.4.14.2 Ala-Ala-4-nitroanilide + H2O - Rattus norvegicus Ala-Ala + 4-nitroaniline - ? 36592 3.4.14.2 Ala-Ala-Ala + H2O 54% activity compared to Met-Ala-Ser Homo sapiens Ala-Ala + Ala - ? 36604 3.4.14.2 Ala-Ala-Ala-Ala + H2O 39% activity compared to Met-Ala-Ser Homo sapiens Ala-Ala + Ala-Ala - ? 14898 3.4.14.2 Ala-Ala-p-nitroanilide + H2O 481% activity compared to Gly-Pro-7-amido-4-methylcoumarin Sus scrofa Ala-Ala + p-nitroaniline - ? 364858 3.4.14.2 Ala-Ala-Phe-OH + H2O - Streptomyces sp. Ala-Ala + Phe-OH - ? 73979 3.4.14.2 Ala-Ala-Phe-OH + H2O - Streptomyces sp. WM-23 Ala-Ala + Phe-OH - ? 73979 3.4.14.2 Ala-Phe-4-nitroanailide + H2O - Streptomyces sp. Ala-Phe + 4-nitroaniline - ? 73944 3.4.14.2 Ala-Phe-4-nitroanailide + H2O - Streptomyces sp. WM-23 Ala-Phe + 4-nitroaniline - ? 73944 3.4.14.2 Ala-Pro-2-(4-methoxy)-naphthylamine + H2O - Cavia porcellus ? - ? 73965 3.4.14.2 Ala-Pro-2-(4-methoxy)-naphthylamine + H2O - Mus musculus ? - ? 73965 3.4.14.2 Ala-Pro-2-(4-methoxy)-naphthylamine + H2O - Homo sapiens ? - ? 73965 3.4.14.2 Ala-Pro-2-(4-methoxy)-naphthylamine + H2O - Rattus norvegicus ? - ? 73965 3.4.14.2 Ala-Pro-4-nitroanilide + H2O - Homo sapiens Ala-Pro + 4-nitroaniline - ? 36580 3.4.14.2 Ala-Pro-amino-4-trifluoromethylcoumarin + H2O - Rattus norvegicus Ala-Pro + amino-4-trifluoromethylcoumarin - ? 364866 3.4.14.2 Ala-Pro-amino-4-trifluoromethylcoumarin + H2O - Homo sapiens Ala-Pro + amino-4-trifluoromethylcoumarin - ? 364866 3.4.14.2 Ala-Pro-p-nitroanilide + H2O - Homo sapiens Ala-Pro + p-nitroaniline - ? 361609 3.4.14.2 Arg-Ala-2-naphthylamide + H2O - Bos taurus Arg-Ala + 2-naphthylamine - ? 73951 3.4.14.2 Arg-Ala-2-naphthylamide + H2O 11% of the activity with Phe-Pro-2-naphthylamide Sus scrofa Arg-Ala + 2-naphthylamine - ? 73951 3.4.14.2 Arg-Pro-2-naphthylamide + H2O 27% of the activity with Phe-Pro-2-naphthylamide Sus scrofa Arg-Pro + 2-naphthylamine - ? 73950 3.4.14.2 Arg-Pro-4-nitroanilide + H2O - Homo sapiens Arg-Pro + 4-nitroaniline - ? 36585 3.4.14.2 Arg-Pro-4-nitroanilide + H2O - Rattus norvegicus Arg-Pro + 4-nitroaniline - ? 36585 3.4.14.2 Arg-Pro-Lys-Pro + H2O fragments of substance P Rattus norvegicus Arg-Pro + Lys-Pro - ? 390173 3.4.14.2 Arg-Pro-Lys-Pro + H2O fragments of substance P Sus scrofa Arg-Pro + Lys-Pro - ? 390173 3.4.14.2 Arg-Pro-Lys-Pro + H2O fragments of substance P Bos taurus Arg-Pro + Lys-Pro - ? 390173 3.4.14.2 Arg-Pro-Lys-Pro + H2O fragments of substance P Homo sapiens Arg-Pro + Lys-Pro - ? 390173 3.4.14.2 Arg-Pro-p-nitroanilide + H2O - Homo sapiens Arg-Pro + p-nitroaniline - ? 361608 3.4.14.2 Arg-Pro-p-nitroanilide + H2O 591% activity compared to Gly-Pro-7-amido-4-methylcoumarin Sus scrofa Arg-Pro + p-nitroaniline - ? 361608 3.4.14.2 Asn-Pro-p-nitroanilide + H2O - Homo sapiens Asn-Pro + p-nitroaniline - ? 384221 3.4.14.2 Asp-Pro-4-nitroanilide + H2O - Homo sapiens Asp-Pro + 4-nitroaniline - ? 73940 3.4.14.2 azocasein + H2O - Odoribacter splanchnicus ? - ? 15347 3.4.14.2 bradykinin + H2O - Rattus norvegicus ? - ? 16889 3.4.14.2 bradykinin + H2O - Sus scrofa ? - ? 16889 3.4.14.2 bradykinin + H2O - Bos taurus ? - ? 16889 3.4.14.2 bradykinin + H2O - Homo sapiens ? - ? 16889 3.4.14.2 bradykinin fragment 1-3 + H2O - Rattus norvegicus ? - ? 73956 3.4.14.2 bradykinin fragment 1-5 + H2O - Rattus norvegicus ? - ? 73957 3.4.14.2 casomorphin + H2O - Rattus norvegicus ? - ? 36621 3.4.14.2 casomorphin + H2O - Sus scrofa ? - ? 36621 3.4.14.2 casomorphin + H2O - Bos taurus ? - ? 36621 3.4.14.2 casomorphin + H2O - Homo sapiens ? - ? 36621 3.4.14.2 Glu-Pro-4-nitroanilide + H2O - Homo sapiens Glu-Pro + 4-nitroaniline - ? 36584 3.4.14.2 Glu-Pro-4-nitroanilide + H2O - Rattus norvegicus Glu-Pro + 4-nitroaniline - ? 36584 3.4.14.2 Gly-Ala-7-amido-4-methylcoumarin + H2O - Homo sapiens Gly-Ala + 7-amino-4-methylcoumarin - ? 361568 3.4.14.2 Gly-Ala-Ala + H2O - Rattus norvegicus Gly-Ala + Ala - ? 73972 3.4.14.2 Gly-L-Pro-4-nitroanilide + H2O - Bos taurus Gly-L-Pro + 4-nitroaniline - ? 405299 3.4.14.2 Gly-Pro-2-(4-methoxy)-naphthylamide + H2O - Sus scrofa ? - ? 73962 3.4.14.2 Gly-Pro-2-naphthylamide + H2O - Odoribacter splanchnicus Gly-Pro + 2-naphthylamine - ? 36560 3.4.14.2 Gly-Pro-4-methylcoumarin 7-amide + H2O - Sus scrofa Gly-Pro + 7-amino-4-methylcoumarin - ? 36563 3.4.14.2 Gly-Pro-4-nitroanilide + H2O - Homo sapiens Gly-Pro + 4-nitroaniline - ? 36559 3.4.14.2 Gly-Pro-4-nitroanilide + H2O - Rattus norvegicus Gly-Pro + 4-nitroaniline - ? 36559 3.4.14.2 Gly-Pro-4-nitroanilide + H2O - Odoribacter splanchnicus Gly-Pro + 4-nitroaniline - ? 36559 3.4.14.2 Gly-Pro-4-nitroanilide + H2O specific for proline Homo sapiens Gly-Pro + 4-nitroaniline - ? 36559 3.4.14.2 Gly-Pro-4-nitroanilide + H2O specific for proline Sus scrofa Gly-Pro + 4-nitroaniline - ? 36559 3.4.14.2 Gly-Pro-7-amido-4-methylcoumarin + H2O - Rattus norvegicus Gly-Pro + 7-amino-4-methylcoumarin - ? 361560 3.4.14.2 Gly-Pro-7-amido-4-methylcoumarin + H2O - Homo sapiens Gly-Pro + 7-amino-4-methylcoumarin - ? 361560 3.4.14.2 Gly-Pro-7-amido-4-methylcoumarin + H2O 100% activity Sus scrofa Gly-Pro + 7-amino-4-methylcoumarin - ? 361560 3.4.14.2 Gly-Pro-Ala + H2O - Rattus norvegicus Gly-Pro + Ala - ? 73970 3.4.14.2 Gly-Pro-Ala + H2O - Sus scrofa Gly-Pro + Ala - ? 73970 3.4.14.2 Gly-Pro-Ala + H2O 96% activity compared to Met-Ala-Ser Homo sapiens Gly-Pro + Ala - ? 73970 3.4.14.2 Gly-Pro-Gly-Gly + H2O 16% activity compared to Met-Ala-Ser Homo sapiens Gly-Pro + Gly-Gly - ? 361570 3.4.14.2 Gly-Pro-Leu-Gly + H2O - Rattus norvegicus ? - ? 73973 3.4.14.2 Gly-Pro-p-nitroanilide + H2O - Homo sapiens Gly-Pro + p-nitroaniline - ? 361612 3.4.14.2 Gly-Pro-p-nitroanilide + H2O 562% activity compared to Gly-Pro-7-amido-4-methylcoumarin Sus scrofa Gly-Pro + p-nitroaniline - ? 361612 3.4.14.2 His-Ala-p-nitroanilide + H2O - Homo sapiens His-Ala + p-nitroaniline - ? 384863 3.4.14.2 His-Pro-p-nitroanilide + H2O - Homo sapiens His-Pro + p-nitroaniline - ? 384865 3.4.14.2 His-Pro-Val + H2O - Rattus norvegicus His-Pro + Val - ? 73968 3.4.14.2 L-Lys-L-Ala-5-chloro-1-anthraquinonylhydrazide-2HBr + H2O i.e. Lys-Ala-CAH, synthetic substrate for colorimetric assay Rattus norvegicus L-Lys-L-Ala + 5-Cl-1-anthraquinonylhydrazine + HBr i.e. CAH, forms a highly insoluble, deeply colored hydrazone when coupled to an aromatic aldehyde, e.g. 4-nitrobenzaldehyde or piperonal, useful for histochemical detection of the enzyme ? 364865 3.4.14.2 L-Lys-L-Ala-7-amido-4-methylcoumarin + H2O - Homo sapiens L-Lys-L-Ala + 7-amino-4-methylcoumarin - ? 452902 3.4.14.2 L-Lys-L-Ala-7-amido-4-methylcoumarin + H2O - Sus scrofa L-Lys-L-Ala + 7-amino-4-methylcoumarin - ? 452902 3.4.14.2 Leu-Ala-2-naphthylamide + H2O - Rattus norvegicus Leu-Ala + 2-naphthylamine - ? 73954 3.4.14.2 Leu-Ala-2-naphthylamide + H2O - Bos taurus Leu-Ala + 2-naphthylamine - ? 73954 3.4.14.2 Lys-Ala-2-(4-methoxy)-naphthylamide Lys-Ala-4-methoxy-2-naphthylamide + H2O - Cavia porcellus ? - ? 73961 3.4.14.2 Lys-Ala-2-(4-methoxy)-naphthylamide Lys-Ala-4-methoxy-2-naphthylamide + H2O - Sus scrofa ? - ? 73961 3.4.14.2 Lys-Ala-2-naphthylamide + H2O - Rattus norvegicus Lys-Ala + 2-naphthylamine - ? 36550 3.4.14.2 Lys-Ala-2-naphthylamide + H2O - Bos taurus Lys-Ala + 2-naphthylamine - ? 36550 3.4.14.2 Lys-Ala-2-naphthylamide + H2O 15% of the activity with Phe-Pro-2-naphthylamide Sus scrofa Lys-Ala + 2-naphthylamine - ? 36550 3.4.14.2 Lys-Ala-4-methylcoumaryl-7-amide + H2O - Homo sapiens Lys-Ala + 7-amino-4-methylcoumarin - ? 73958 3.4.14.2 Lys-Ala-4-methylcoumaryl-7-amide + H2O - Sus scrofa Lys-Ala + 7-amino-4-methylcoumarin - ? 73958 3.4.14.2 Lys-Ala-7-amido-4-methylcoumarin + H2O - Rattus norvegicus Lys-Ala + 7-amino-4-methylcoumarin - ? 361569 3.4.14.2 Lys-Ala-7-amido-4-methylcoumarin + H2O - Homo sapiens Lys-Ala + 7-amino-4-methylcoumarin - ? 361569 3.4.14.2 Lys-Ala-7-amido-4-methylcoumarin + H2O 38% activity compared to Gly-Pro-7-amido-4-methylcoumarin Sus scrofa Lys-Ala + 7-amino-4-methylcoumarin - ? 361569 3.4.14.2 Lys-Ala-Ala + H2O - Sus scrofa Lys-Ala + Ala - ? 73971 3.4.14.2 Lys-Ala-Ala + H2O - Bos taurus Lys-Ala + Ala - ? 73971 3.4.14.2 Lys-Ala-OCH3 + H2O - Bos taurus ? - ? 73983 3.4.14.2 Lys-Ala-p-nitroanilide + H2O - Homo sapiens Lys-Ala + p-nitroaniline - ? 385105 3.4.14.2 Lys-Ala-Pro + H2O - Bos taurus Lys-Ala + Pro - ? 73974 3.4.14.2 Lys-Arg-2-naphthylamide + H2O - Dictyostelium discoideum Lys-Arg + 2-naphthylamine - ? 73953 3.4.14.2 Lys-Pro-2-(4-methoxy)-naphthylamide + H2O - Bos taurus ? - ? 73963 3.4.14.2 Lys-Pro-2-(4-methoxy)-naphthylamine + H2O - Cavia porcellus ? - ? 73964 3.4.14.2 Lys-Pro-2-(4-methoxy)-naphthylamine + H2O - Mus musculus ? - ? 73964 3.4.14.2 Lys-Pro-2-(4-methoxy)-naphthylamine + H2O - Homo sapiens ? - ? 73964 3.4.14.2 Lys-Pro-2-(4-methoxy)-naphthylamine + H2O - Rattus norvegicus ? - ? 73964 3.4.14.2 Lys-Pro-2-(4-methoxy)-naphthylamine + H2O - Oryctolagus cuniculus ? - ? 73964 3.4.14.2 Lys-Pro-2-naphthylamide + H2O - Bos taurus Lys-Pro + 2-naphthylamine - ? 73952 3.4.14.2 Lys-Pro-2-naphthylamide + H2O 46% of the activity with Phe-Pro-2-naphthylamide Sus scrofa Lys-Pro + 2-naphthylamine - ? 73952 3.4.14.2 Lys-Pro-4-nitroanilide + H2O - Homo sapiens Lys-Pro + 4-nitroaniline - ? 36579 3.4.14.2 Lys-Pro-4-nitroanilide + H2O - Rattus norvegicus Lys-Pro + 4-nitroaniline - ? 36579 3.4.14.2 Lys-Pro-7-amido-4-methylcoumarin + H2O - Homo sapiens Lys-Pro + 7-amino-4-methylcoumarin - ? 361566 3.4.14.2 Lys-Pro-p-nitroanilide + H2O - Homo sapiens Lys-Pro + p-nitroaniline - ? 361607 3.4.14.2 Met-Ala-Ser + H2O 100% activity Homo sapiens Met-Ala + Ser - ? 392583 3.4.14.2 Met-Met-Ala + H2O - Bos taurus Met-Met + Ala - ? 73975 3.4.14.2 Met-Met-Met + H2O - Bos taurus Met-Met + Met - ? 36506 3.4.14.2 additional information substrate specificity Rattus norvegicus ? - ? 89 3.4.14.2 additional information Pro and Arg are the preferred penultimate residues Bos taurus ? - ? 89 3.4.14.2 additional information no hydrolysis occurs if either the carboxyl group or the amino group of otherwise susceptible tripeptides is blocked. Tetrapeptides, tripeptide esters and dipeptides are not attacked Bos taurus ? - ? 89 3.4.14.2 additional information no activity with bradykinin Rattus norvegicus ? - ? 89 3.4.14.2 additional information specific for Pro at position P1 Homo sapiens ? - ? 89 3.4.14.2 additional information the enzyme contributes to the alveolar macrophage's proteolytic capabilities Rattus norvegicus ? - ? 89 3.4.14.2 additional information the enzyme contributes to the alveolar macrophage's proteolytic capabilities Oryctolagus cuniculus ? - ? 89 3.4.14.2 additional information hydrolyzes specifically N-terminal X-alanine or X-proline from their respective arylamides and from tripeptides or tetrapeptides, but not from pentapeptides Rattus norvegicus ? - ? 89 3.4.14.2 additional information possible role of the mast cell enzyme in remodelling of connective tissue Rattus norvegicus ? - ? 89 3.4.14.2 additional information the enzyme may play a role in the segregation and degradation of specific regions of normal lens fiber cells Rattus norvegicus ? - ? 89 3.4.14.2 additional information Ala and Pro are the preferred residues in the P1 position Rattus norvegicus ? - ? 89 3.4.14.2 additional information Ala and Pro are the preferred residues in the P1 position Sus scrofa ? - ? 89 3.4.14.2 additional information Ala and Pro are the preferred residues in the P1 position Bos taurus ? - ? 89 3.4.14.2 additional information Ala and Pro are the preferred residues in the P1 position Homo sapiens ? - ? 89 3.4.14.2 additional information no activity with Ala-Ala-Ala-Ala-Ala, Arg-Pro-Lys-Pro (substance P 1-4), and Arg-Pro-Pro-Gly-Phe (bradikinin1-5) Homo sapiens ? - ? 89 3.4.14.2 additional information no activity with Gly-Arg-7-amido-4-methylcoumarin, Arg-Arg-7-amido-4-methylcoumarin, and Ala-Arg-7-amido-4-methylcoumarin Sus scrofa ? - ? 89 3.4.14.2 Nle-Nle-p-nitroanilide + H2O - Homo sapiens Nle-Nle + p-nitroaniline - ? 385412 3.4.14.2 Nle-Nle-rhodamine + H2O - Homo sapiens Nle-Nle + rhodamine - ? 364869 3.4.14.2 Nle-Pro-7-amido-4-methylcoumarin + H2O - Homo sapiens Nle-Pro + 7-amino-4-methylcoumarin - ? 364868 3.4.14.2 Nle-Pro-rhodamine + H2O - Homo sapiens Nle-Pro + rhodamine - ? 364870 3.4.14.2 Phe-Pro-2-naphthylamide + H2O - Rattus norvegicus Phe-Pro + 2-naphthylamine - ? 36571 3.4.14.2 Phe-Pro-2-naphthylamide + H2O - Sus scrofa Phe-Pro + 2-naphthylamine - ? 36571 3.4.14.2 Phe-Pro-4-methylcoumarin 7-amide + H2O - Homo sapiens Phe-Pro + 7-amino-4-methylcoumarin - ? 36570 3.4.14.2 Phe-Pro-Ala + H2O - Sus scrofa Phe-Pro + Ala - ? 73969 3.4.14.2 Phe-Pro-p-nitroanilide + H2O - Homo sapiens Phe-Pro + p-nitroaniline - ? 361611 3.4.14.2 Pro-Ala-p-nitroanilide + H2O - Homo sapiens Pro-Ala + p-nitroaniline - ? 385584 3.4.14.2 Ser-Met-Gln + H2O - Bos taurus Ser-Met + Gln - ? 73978 3.4.14.2 Ser-Met-Glu + H2O - Bos taurus Ser-Met + Glu - ? 36505 3.4.14.2 Ser-Met-OCH3 + H2O - Bos taurus ? - ? 73982 3.4.14.2 Ser-Pro-p-nitroanilide + H2O - Homo sapiens Ser-Pro + p-nitroaniline - ? 361610 3.4.14.2 Substance P + H2O - Rattus norvegicus ? - ? 17205 3.4.14.2 Substance P + H2O - Sus scrofa ? - ? 17205 3.4.14.2 Substance P + H2O - Bos taurus ? - ? 17205 3.4.14.2 Substance P + H2O - Homo sapiens ? - ? 17205 3.4.14.2 Tyr-Ala-p-nitroanilide + H2O - Homo sapiens Tyr-Ala + p-nitroaniline - ? 385864 3.4.14.2 Val-Ala-4-nitroanilide + H2O - Odoribacter splanchnicus Val-Ala + 4-nitroaniline - ? 73938 3.4.14.2 Val-Ala-Phe-OH + H2O - Streptomyces sp. Val-Ala + Phe-OH - ? 73980 3.4.14.2 Val-Ala-Phe-OH + H2O - Streptomyces sp. WM-23 Val-Ala + Phe-OH - ? 73980