3.4.11.14 Ala-2-naphthylamide + H2O - Homo sapiens Ala + 2-naphthylamine - ? 36241 3.4.11.14 Ala-2-naphthylamide + H2O - Rattus norvegicus Ala + 2-naphthylamine - ? 36241 3.4.11.14 Ala-4-methyl-coumaryl-7-amide + H2O - Homo sapiens Ala + 7-amino-4-methylcoumarin - ? 418446 3.4.11.14 Ala-4-nitroanilide + H2O - Homo sapiens ? - ? 361351 3.4.11.14 Ala-7-amido-4-methylcoumarin - Rattus norvegicus Ala + 7-amino-4-methylcoumarin - ? 361352 3.4.11.14 Ala-Ala + H2O 45.4% of the activity with Ala-Trp Homo sapiens Ala + Ala - ? 36448 3.4.11.14 Ala-Asp + H2O 11.4% of the activity with Ala-Trp Homo sapiens Ala + Asp - ? 361375 3.4.11.14 Ala-Glu + H2O 19.0% of the activity with Ala-Trp Homo sapiens Ala + Glu - ? 361373 3.4.11.14 Ala-Gly + H2O 17.8% of the activity with Ala-Trp Homo sapiens Ala + Gly - ? 36249 3.4.11.14 Ala-Leu + H2O 27.1% of the activity with Ala-Trp Homo sapiens Ala + Leu - ? 64874 3.4.11.14 Ala-p-nitroanilide + H2O - Homo sapiens Ala + p-nitroaniline - ? 36246 3.4.11.14 Ala-p-nitroanilide + H2O - Pseudomonas aeruginosa L-alanine + p-nitroaniline - ? 381359 3.4.11.14 Ala-p-nitroanilide + H2O - Acinetobacter calcoaceticus L-alanine + p-nitroaniline - ? 381359 3.4.11.14 Ala-Phe + H2O 42.8% of the activity with Ala-Trp Homo sapiens Ala + Phe - ? 36449 3.4.11.14 Ala-Ser + H2O 22.5% of the activity with Ala-Trp Homo sapiens Ala + Ser - ? 361371 3.4.11.14 Ala-Trp + H2O - Homo sapiens Ala + Trp - ? 358885 3.4.11.14 Ala-Val + H2O 47.3% of the activity with Ala-Trp Homo sapiens Ala + Val - ? 361365 3.4.11.14 Aplysia insulin D + H2O Aplysia insulin D degrades slowly under the effects of mAAP Sus scrofa ? - ? 391296 3.4.11.14 Arg-2-naphthylamide + H2O - Homo sapiens Arg + 2-naphthylamine - ? 355548 3.4.11.14 Arg-2-naphthylamide + H2O - Rattus norvegicus Arg + 2-naphthylamine - ? 355548 3.4.11.14 Arg-2-naphthylamide + H2O 28% of the activity with Ala-2-naphthylamide Homo sapiens Arg + 2-naphthylamine - ? 355548 3.4.11.14 Arg-7-amido-4-methylcoumarin 36% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus Arg + 7-amino-4-methylcoumarin - ? 361355 3.4.11.14 Arg-Arg-7-amido-4-methylcoumarin 13% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus ? - ? 361361 3.4.11.14 CKKQ20KK + H2O - Homo sapiens ? - ? 391668 3.4.11.14 Gly-2-naphthylamide + H2O 13.7% of the activity with Ala-2-naphthylamide Homo sapiens Gly + 2-naphthylamine - ? 36244 3.4.11.14 glycine-4-methyl-coumaryl-7-amide + H2O - Danio rerio glycine + 7-amino-4-methylcoumarin - ? 418699 3.4.11.14 Ile-2-naphthylamide + H2O - Homo sapiens Ile + 2-naphthylamide - ? 361341 3.4.11.14 KKE-(5-[(2-aminoethyl) amino]-naphthalene-1-sulfonic acid)-Q9K-((4'-dimethylaminoazobenzene-4'-sulfonyl))-K + H2O - Homo sapiens ? - ? 392313 3.4.11.14 KKQ30KK + H2O - Homo sapiens ? - ? 392316 3.4.11.14 L-Ala-2-naphthylamide + H2O - Rattus norvegicus L-Ala + 2-naphthylamine - ? 36267 3.4.11.14 L-alanine-4-methylcoumaryl-7-amide + H2O - Danio rerio L-alanine + 7-amino-4-methylcoumarin - ? 392343 3.4.11.14 L-Arg-enkephalin + H2O - Mus musculus L-Arg + enkephalin - ? 392347 3.4.11.14 L-arginine-4-methylcoumaryl-7-amide + H2O - Danio rerio L-arginine + 7-amino-4-methylcoumarin - ? 392349 3.4.11.14 L-leucine-4-methylcoumaryl-7-amide + H2O - Danio rerio L-leucine + 7-amino-4-methylcoumarin - ? 392386 3.4.11.14 L-lysine-4-methylcoumaryl-7-amide + H2O - Danio rerio L-lysine + 7-amino-4-methylcoumarin - ? 392391 3.4.11.14 L-methionine-4-methylcoumaryl-7-amide + H2O - Danio rerio L-methionine + 7-amino-4-methylcoumarin - ? 392395 3.4.11.14 L-proline-4-methylcoumaryl-7-amide + H2O - Danio rerio L-proline + 7-amino-4-methylcoumarin - ? 418773 3.4.11.14 L-tyrosine-4-methylcoumaryl-7-amide + H2O - Danio rerio L-tyrosine + 7-amino-4-methylcoumarin - ? 418778 3.4.11.14 L-valine-4-methylcoumaryl-7-amide + H2O - Danio rerio L-valine + 7-amino-4-methylcoumarin - ? 418781 3.4.11.14 Leu-(beta-naphthylamine) + H2O - Homo sapiens Leu + naphthylamine - ? 418783 3.4.11.14 Leu-2-naphthylamide + H2O - Homo sapiens Leu + 2-naphthylamine - ? 36242 3.4.11.14 Leu-2-naphthylamide + H2O 36.4% of the activity with Ala-2-naphthylamide Homo sapiens Leu + 2-naphthylamine - ? 36242 3.4.11.14 Leu-4-methyl-coumaryl-7-amide + H2O - Homo sapiens Leu + 7-amino-4-methylcoumarin - ? 418784 3.4.11.14 Leu-7-amido-4-methylcoumarin 90% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus Leu + 7-amino-4-methylcoumarin - ? 361357 3.4.11.14 Leu-Ala + H2O 25.6% of the activity with Ala-Trp Homo sapiens Leu + Ala - ? 36251 3.4.11.14 Leu-Gly + H2O 5.5% of the activity with Ala-Trp Homo sapiens Leu + Gly - ? 36252 3.4.11.14 Leu-Leu + H2O - Homo sapiens Leu + Leu - ? 64754 3.4.11.14 Leu-Leu + H2O 35.9% of the activity with Ala-Trp Homo sapiens Leu + Leu - ? 64754 3.4.11.14 Leu-p-nitroanilide + H2O - Homo sapiens Leu + p-nitroaniline - ? 36247 3.4.11.14 Lys-2-naphthylamide + H2O - Homo sapiens Lys + 2-naphthylamine - ? 36318 3.4.11.14 Lys-2-naphthylamide + H2O 13.4% of the activity with Ala-2-naphthylamide Homo sapiens Lys + 2-naphthylamine - ? 36318 3.4.11.14 Lys-7-amido-4-methylcoumarin 93% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus Lys + 7-amino-4-methylcoumarin - ? 361356 3.4.11.14 Lys-Ala-7-amido-4-methylcoumarin 79% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus ? - ? 361360 3.4.11.14 Lys-p-nitroanilide + H2O - Homo sapiens Lys + p-nitroaniline - ? 36248 3.4.11.14 Met-2-naphthylamide + H2O - Homo sapiens Met + 2-naphthylamine - ? 36243 3.4.11.14 Met-2-naphthylamide + H2O 57.5% of the activity with Ala-2-naphthylamide Homo sapiens Met + 2-naphthylamine - ? 36243 3.4.11.14 Met-7-amido-4-methylcoumarin 97% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus Met + 7-amino-4-methylcoumarin - ? 361354 3.4.11.14 Met-enkephalin + H2O - Rattus norvegicus ? releases only the N-terminal tyrosine ? 116055 3.4.11.14 Met-Lys-bradykinin + H2O - Homo sapiens Met + Lys + bradykinin - ? 361363 3.4.11.14 Met-p-nitroanilide + H2O - Homo sapiens Met + p-nitroaniline - ? 392584 3.4.11.14 additional information there are two active centre ionizable groups with pKa values of approximately 6.0 and 7.5 which are involved in substrate binding or inhibitory amino acid binding Homo sapiens ? - ? 89 3.4.11.14 additional information no activity with: Gly-Pro-7-amido-4-methylcoumarin, butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin, butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, butyloxycarbonyl-Val–Pro-Arg-7-amido-4-methylcoumarin,succinyl-Ala-Ala-Ala-7-amido-4-methylcoumarin, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, succinyl-Gly-Pro-7-amido-4-methylcoumarin, succinyl-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin, succinyl-Ile-Ile-Trp-7-amido-4-methylcoumarin, benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin Rattus norvegicus ? - ? 89 3.4.11.14 additional information does not digest Asn-Trp-Phe-NH2, Ala-Pro-Arg-Leu-Arg-Phe-Tyr-Ser, and angiotensin I Sus scrofa ? - ? 89 3.4.11.14 additional information mAAP digests amino acids in the following approximate order: Ala > Phe > Tyr > Leu > Arg > Thr > Trp > Lys > Ser > Asp > His > Val Sus scrofa ? - ? 89 3.4.11.14 Phe-2-naphthylamide + H2O - Homo sapiens Phe + 2-naphthylamine - ? 361347 3.4.11.14 Phe-2-naphthylamide + H2O 62.8% of the activity with Ala-2-naphthylamide Homo sapiens Phe + 2-naphthylamine - ? 361347 3.4.11.14 Phe-7-amido-4-methylcoumarin 84% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus Phe + 7-amino-4-methylcoumarin - ? 361358 3.4.11.14 Phe-Gly + H2O 19.2% of the activity with Ala-Trp Homo sapiens Phe + Gly - ? 36250 3.4.11.14 poly-Glu peptide + H2O capable of rapidly digesting poly-Q sequences Homo sapiens ? - ? 393274 3.4.11.14 Pro-7-amido-4-methylcoumarin 39% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus Pro + 7-amino-4-methylcoumarin - ? 361359 3.4.11.14 Pro-p-nitroanilide + H2O - Homo sapiens Pro + p-nitroaniline - ? 382665 3.4.11.14 Pro-Phe-Arg-7-amido-4-methylcoumarin 20% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus ? - ? 361362 3.4.11.14 Ser-2-naphthylamide + H2O 6.0% of the activity with Ala-2-naphthylamide Homo sapiens Ser + 2-naphthylamine - ? 361349 3.4.11.14 tau protein + H2O - Homo sapiens ? - ? 393638 3.4.11.14 Thr-2-naphthylamide + H2O 5.7% of the activity with Ala-2-naphthylamide Homo sapiens Thr + 2-naphthylamine - ? 361350 3.4.11.14 Trp-2-naphthylamide + H2O 19.2% of the activity with Ala-2-naphthylamide Homo sapiens Trp + 2-naphthylamine - ? 361343 3.4.11.14 Tyr-7-amido-4-methylcoumarin + H2O 99% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus Tyr + 7-amino-4-methylcoumarin - ? 361353 3.4.11.14 Val-2-naphthylamide + H2O - Homo sapiens Val + 2-naphthylamine - ? 361340 3.4.11.14 Val-p-nitroanilide + H2O - Homo sapiens Val + p-nitroaniline - ? 393812