3.3.2.6 (7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O - Mus musculus (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate - ? 393911 3.3.2.6 (7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O - Homo sapiens (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate - ? 393911 3.3.2.6 (7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O - Cricetulus sp. (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate - ? 393911 3.3.2.6 Ala-4-nitroanilide + H2O - Homo sapiens Ala + 4-nitroaniline - ? 36589 3.3.2.6 Arg-Ala-Arg + H2O - Homo sapiens ? - ? 404352 3.3.2.6 Arg-Ser-Arg + H2O - Homo sapiens ? - ? 404359 3.3.2.6 L-Ala-4-nitroanilide + H2O - Homo sapiens L-Ala + 4-nitroaniline - ? 382096 3.3.2.6 L-Ala-p-nitroanilide + H2O - Homo sapiens L-Ala + p-nitroaniline - ? 381360 3.3.2.6 L-alanine 2-naphthylamide + H2O - Saccharomyces cerevisiae L-alanine + 2-naphthylamine - ? 417131 3.3.2.6 L-alanine-4-nitroanilide + H2O - Mus musculus L-alanine + 4-nitroaniline - ? 364854 3.3.2.6 L-alanine-4-nitroanilide + H2O - Homo sapiens L-alanine + 4-nitroaniline - ? 364854 3.3.2.6 L-alanine-4-nitroanilide + H2O - Xenopus laevis L-alanine + 4-nitroaniline - ? 364854 3.3.2.6 L-alanine-7-amido-4-methylcoumarin + H2O - Homo sapiens L-alanine + 7-amino-4-methylcoumarin - ? 431991 3.3.2.6 L-Arg-p-nitroanilide + H2O - Homo sapiens L-Arg + p-nitroaniline - ? 36321 3.3.2.6 L-arginine 2-naphthylamide + H2O - Saccharomyces cerevisiae L-arginine + 2-naphthylamine - ? 417137 3.3.2.6 L-arginine-4-nitroanilide + H2O - Homo sapiens L-arginine + 4-nitroaniline - ? 418749 3.3.2.6 L-arginine-7-amido-4-methylcoumarin + H2O - Homo sapiens L-arginine + 7-amino-4-methylcoumarin - ? 432003 3.3.2.6 L-glutamate-7-amido-4-methylcoumarin + H2O - Homo sapiens L-glutamate + 7-amino-4-methylcoumarin - ? 432015 3.3.2.6 L-histidine-7-amido-4-methylcoumarin + H2O - Homo sapiens L-histidine + 7-amino-4-methylcoumarin - ? 432022 3.3.2.6 L-isoleucine 2-naphthylamide + H2O - Saccharomyces cerevisiae L-isoleucine + 2-naphthylamine - ? 417157 3.3.2.6 L-leucine 2-naphthylamide + H2O - Saccharomyces cerevisiae L-leucine + 2-naphthylamine - ? 417161 3.3.2.6 L-leucine-4-nitroanilide + H2O - Homo sapiens L-leucine + 4-nitroaniline - ? 364849 3.3.2.6 L-leucine-4-nitroanilide + H2O - Saccharomyces cerevisiae L-leucine + 4-nitroaniline - ? 364849 3.3.2.6 L-leucine-7-amido-4-methylcoumarin + H2O - Homo sapiens L-leucine + 7-amino-4-methylcoumarin - ? 418764 3.3.2.6 L-lysine-7-amido-4-methylcoumarin + H2O - Homo sapiens L-lysine + 7-amino-4-methylcoumarin - ? 432039 3.3.2.6 L-methionine 2-naphthylamide + H2O - Saccharomyces cerevisiae L-methionine + 2-naphthylamine - ? 417168 3.3.2.6 L-methionine-7-amido-4-methylcoumarin + H2O - Homo sapiens L-methionine + 7-amino-4-methylcoumarin - ? 392396 3.3.2.6 L-phenylalanine-7-amido-4-methylcoumarin + H2O - Homo sapiens L-phenylalanine + 7-amino-4-methylcoumarin - ? 432048 3.3.2.6 L-Pro-Gly-L-Pro + H2O - Homo sapiens ? - ? 432049 3.3.2.6 L-proline-7-amido-4-methylcoumarin + H2O - Homo sapiens L-proline + 7-amino-4-methylcoumarin - ? 412328 3.3.2.6 L-serine-7-amido-4-methylcoumarin + H2O - Homo sapiens L-serine + 7-amino-4-methylcoumarin - ? 432053 3.3.2.6 L-tyrosine-7-amido-4-methylcoumarin + H2O - Homo sapiens L-tyrosine + 7-amino-4-methylcoumarin - ? 432058 3.3.2.6 L-Val-4-nitroanilide + H2O - Homo sapiens L-Val + 4-nitroaniline - ? 382188 3.3.2.6 L-valine 2-naphthylamide + H2O - Saccharomyces cerevisiae L-valine + 2-naphthylamine - ? 417206 3.3.2.6 leukotriene A4 + H2O - Cavia porcellus leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O - Mus musculus leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O - Homo sapiens leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O - Rattus norvegicus leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O - Canis lupus familiaris leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O - Xenopus laevis leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O - Danio rerio leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O - Canis lupus familiaris leukotriene B4 i.e. LTB4, a potent inflammatory mediator originating from membrane-derived arachidonic acid ? 361325 3.3.2.6 leukotriene A4 + H2O - Homo sapiens leukotriene B4 i.e. LTB4, a powerful proinflammatory agent ? 361325 3.3.2.6 leukotriene A4 + H2O - Mus musculus leukotriene B4 LTB4 is an endogenous itch mediator in the skin and is involved in the pruritus response in allergic reactions ? 361325 3.3.2.6 leukotriene A4 + H2O - Mus musculus C57BL/6 leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O - Mus musculus C57BL/6J leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O - Saccharomyces cerevisiae (5S,6S)-dihydroxy-7,9-trans-11,14-cis-eicosatetraenoic acid i.e. DHETE ? 361334 3.3.2.6 leukotriene A4 + H2O - Xenopus laevis leukotriene B4 + (5S,12R)-dihydroxy-6,10-trans-8,14,cis-eicosatetraenoic acid 90% leukotriene B4 and 10% (5S,12R)-dihydroxy-6,10-trans-8,14,cis-eicosatetraenoic acid ? 361335 3.3.2.6 leukotriene A4 + H2O - Saccharomyces cerevisiae (5S,6S)-dihydroxy-7,9-trans-11,14-cis-eicosatetraenoic acid + leukotriene B4 + (5S,12R)-dihydroxy-6,10-trans-8,14,cis-eicosatetraenoic acid when large amounts of enzyme are incubated with leukotriene A4: 72% (5S,6S)-dihydroxy-7,9-trans-11,14-cis-eicosatetraenoic acid, 18% leukotriene B4 and 10% (5S,12R)-dihydroxy-6,10-trans-8,14,cis-eicosatetraenoic acid ? 361336 3.3.2.6 leukotriene A4 + H2O - Xenopus laevis leukotriene B4 + 5(S),12R-dihydroxy-6,10-trans-8,14-ciseicosatetraenoic acid product ratio 9:1 ? 431052 3.3.2.6 lysine-4-nitroanilide + H2O - Homo sapiens lysine + 4-nitroaniline - ? 361331 3.3.2.6 additional information - Cavia porcellus ? - ? 89 3.3.2.6 additional information - Mus musculus ? - ? 89 3.3.2.6 additional information - Homo sapiens ? - ? 89 3.3.2.6 additional information - Saccharomyces cerevisiae ? - ? 89 3.3.2.6 additional information - Xenopus laevis ? - ? 89 3.3.2.6 additional information - Caenorhabditis elegans ? - ? 89 3.3.2.6 proline-4-nitroanilide + H2O - Homo sapiens proline + 4-nitroaniline - ? 361329 3.3.2.6 L-Ala-4-nitroanilide + H2O aminopeptidase activity Mus musculus L-Ala + 4-nitroaniline - ? 382096 3.3.2.6 L-arginine-7-amido-4-methylcoumarine + H2O an artificial substrate Homo sapiens L-arginine + 4-methylcoumarin-7-amine - ? 450139 3.3.2.6 L-Arg-L-Ala-L-Arg + H2O bi-functional enzyme that also exhibits aminopeptidase activity with a preference over arginyl tripeptides Homo sapiens ? - ? 431999 3.3.2.6 leukotriene A4 + H2O biosynthesis of leukotriene B4 Homo sapiens leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O biosynthesis of leukotriene B4 Rattus norvegicus leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O biosynthesis of leukotriene B4 Cavia porcellus leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O central enzyme in leukotriene B4 formation, it is likely to be involved in transcellular leukotriene formation Homo sapiens leukotriene B4 - ? 361325 3.3.2.6 additional information either glutamic or glutamine moiety at amino acid 297 is required for full LTA4 hydrolase activity, free carboxylic acid of glutamic acid is essential for aminopeptidase Homo sapiens ? - ? 89 3.3.2.6 leukotriene A4 + H2O highly specific Rattus norvegicus leukotriene B4 - ? 361325 3.3.2.6 leukotriene A5 + H2O i.e. 5(S)-trans-5,6-oxido-7,9-trans-11,14,17-cis-eicosatetraenoic acid Homo sapiens ? - ? 361326 3.3.2.6 leukotriene A5 + H2O i.e. 5(S)-trans-5,6-oxido-7,9-trans-11,14,17-cis-eicosatetraenoic acid Rattus norvegicus ? - ? 361326 3.3.2.6 leukotriene A4 + H2O i.e. 5(S)-trans-5,6-oxido-7,9-trans-11,14-cis-eicosatetraenoic acid Cavia porcellus leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O leukotriene A4 hydrolase catalyzes the final step in the synthesis of leukotriene B4, a potent chemoattractant and proinflammatory eicosanoid Homo sapiens leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O leukotriene A4 hydrolase catalyzes the final step in the synthesis of leukotriene B4, a potent chemoattractant and proinflammatory eicosanoid. LTB4 functions as a macrophage chemoattractant in the zebrafish, injection into the hindbrain ventricle of wild-type arvae induces rapid recruitment of macrophages Danio rerio leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O LTA4 Homo sapiens leukotriene B4 LTB4 ? 361325 3.3.2.6 additional information LTA4H is a zinc metalloprotease Homo sapiens ? - ? 89 3.3.2.6 additional information LTA4H is a zinc metalloprotease Rattus norvegicus ? - ? 89 3.3.2.6 additional information LTA4H is a zinc metalloprotease Canis lupus familiaris ? - ? 89 3.3.2.6 leukotriene A4 + H2O model for the binding of leukotriene A4 to the active site Saccharomyces cerevisiae leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O model for the binding of leukotriene A4 to the active site Xenopus laevis leukotriene B4 - ? 361325 3.3.2.6 leukotriene A4 + H2O model for the binding of leukotriene A4 to the active site Cavia porcellus leukotriene B4 mutants of Tyr378 are able to generate, not only leukotriene B4, but also (5S,12R)-dihydroxy-6,10-trans-8,14-cis-eicosatetraenoic acid, in a yield of about 20-30% ? 361325 3.3.2.6 leukotriene A4 + H2O model for the binding of leukotriene A4 to the active site Mus musculus leukotriene B4 mutants of Tyr378 are able to generate, not only leukotriene B4, but also (5S,12R)-dihydroxy-6,10-trans-8,14-cis-eicosatetraenoic acid, in a yield of about 20-30% ? 361325 3.3.2.6 leukotriene A4 + H2O model for the binding of leukotriene A4 to the active site Homo sapiens leukotriene B4 mutants of Tyr378 are able to generate, not only leukotriene B4, but also (5S,12R)-dihydroxy-6,10-trans-8,14-cis-eicosatetraenoic acid, in a yield of about 20-30% ? 361325 3.3.2.6 leukotriene A4 + H2O model for the binding of leukotriene A4 to the active site Rattus norvegicus leukotriene B4 mutants of Tyr378 are able to generate, not only leukotriene B4, but also (5S,12R)-dihydroxy-6,10-trans-8,14-cis-eicosatetraenoic acid, in a yield of about 20-30% ? 361325 3.3.2.6 additional information neutrophil LTA4 hydrolase also converts LTA5 to LTB5, less efficiently than LTA4 to LTB4 Homo sapiens ? - ? 89 3.3.2.6 additional information no activity with L-Asn, L-Asp, L-Glu, Gly, L-Ile, L-Thr, L-Trp, L-Val, as well as D-amino acids Homo sapiens ? - ? 89 3.3.2.6 additional information not: glycine-4-nitroanilide, glutamyl-4-nitroanilide, valine-4-nitroanilide Homo sapiens ? - ? 89 3.3.2.6 leukotriene A4 + H2O one of the physiologically important processes in the arachidonic acid biosynthetic pathway, leukotriene B4 is a proinflammatory mediator which stimulates adhesion of circulating neutrophils to vascular endothelium and directs their migration towards sites of inflammation Homo sapiens leukotriene B4 - ? 361325 3.3.2.6 proline-glycine-proline + H2O PGP Homo sapiens ? - ? 450514 3.3.2.6 additional information possible proteolytic function of the enzyme may be limited to the extracellular space Homo sapiens ? - ? 89 3.3.2.6 additional information proline-glycine-proline (PGP) is a substrate for the enzyme's aminopeptidase (AP) activity Rattus norvegicus ? - ? 89 3.3.2.6 additional information proline-glycine-proline (PGP) is a substrate for the enzyme's aminopeptidase (AP) activity Homo sapiens ? - ? 89 3.3.2.6 (7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O substrate for epoxide hydrolase activity Saccharomyces cerevisiae (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate - ? 393911 3.3.2.6 (7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O substrate for the epoxide hydrolase reaction, in addition LTA4H shows a peptidase activity Homo sapiens (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate - ? 393911 3.3.2.6 additional information substrates docking and molecular dynamics simulations, interaction analysis. The binding cavity of LTA4H is formed by H299, H295, E318, and Zn2+. Residues R563 and K565 in the C-terminal domain of LTA4H are key residues involved in carboxylate recognition from endogenous substrates of the enzyme Homo sapiens ? - ? 89 3.3.2.6 additional information the aminopeptidase activity accepts a variety of substrates and certain arginyl di- and tri-peptides as well as p-nitroanilide derivates of Ala and Arg Cavia porcellus ? - ? 89 3.3.2.6 additional information the aminopeptidase activity accepts a variety of substrates and certain arginyl di- and tri-peptides as well as p-nitroanilide derivates of Ala and Arg Mus musculus ? - ? 89 3.3.2.6 additional information the aminopeptidase activity accepts a variety of substrates and certain arginyl di- and tri-peptides as well as p-nitroanilide derivates of Ala and Arg Homo sapiens ? - ? 89 3.3.2.6 additional information the aminopeptidase activity accepts a variety of substrates and certain arginyl di- and tri-peptides as well as p-nitroanilide derivates of Ala and Arg Rattus norvegicus ? - ? 89 3.3.2.6 additional information the aminopeptidase binding site shares a similar structure to LTA4H at its ligand binding sites Homo sapiens ? - ? 89 3.3.2.6 additional information the aminopeptidase binding site shares a similar structure to LTA4H at its ligand binding sites Mus musculus ? - ? 89 3.3.2.6 L-arginine + H2O the best proteinogenic amino acid recognized by the enzyme is L-arginine Homo sapiens ? - ? 369827