3.2.2.20 3-methyladenine residues in alkylated DNA + H2O - Escherichia coli 3-methyladenine + ? - ? 452852 3.2.2.20 3-methyladenine residues in alkylated DNA + H2O - Escherichia coli BW 9062 3-methyladenine + ? - ? 452852 3.2.2.20 5'-CGATAGCATCCT[hypoxanthine]CCTTCTCTCCAT-3' + H2O - Mycobacterium tuberculosis variant bovis ? - ? 431419 3.2.2.20 alkylated DNA + H2O - Staphylococcus aureus 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Mus musculus 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Escherichia coli 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Homo sapiens 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Saccharomyces cerevisiae 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Mycolicibacterium smegmatis 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Pseudomonas fluorescens 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Bacillus cereus 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Schizosaccharomyces pombe 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Mycobacterium tuberculosis 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Leptospira interrogans 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Escherichia coli 3-methyladenine + ? releases 3-methyladenine and 3-ethyladenine ? 361297 3.2.2.20 alkylated DNA + H2O - Escherichia coli 3-methyladenine + ? also releases a minmal amount of 3-methylguanine ? 361297 3.2.2.20 alkylated DNA + H2O - Mus musculus 3-methyladenine + ? also removes hypoxanthine and 1,N6-ethenoadenine from DNA, dependent on the structure ? 361297 3.2.2.20 alkylated DNA + H2O calf thymus DNA Escherichia coli 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O calf thymus DNA Micrococcus luteus 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O calf thymus DNA Bacillus subtilis 3-methyladenine + ? prefers to release hypoxanthine and 1,N6,ethenoadenine ? 361297 3.2.2.20 alkylated DNA + H2O important role in preventing the mutagenic effects of deaminated purines and cyclic etheno adducts Bacillus subtilis 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O also removes 7-methylguanine Mus musculus 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O smallest member of the helix-hairpin-helix HhH superfamily of DNA glycosylases Escherichia coli 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O double-stranded DNA is an effective substrate, enzyme is less efficient in excision of base damage from single-stranded regions transiently formed in DNA during transcription and replication Escherichia coli 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O cellular repair of alkylated DNA base modifications Micrococcus luteus 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O reaction of the enzyme with alkylated DNA leads to introduction of apurinic sites but no chain breaks Escherichia coli 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O nuclear transcription factor estrogen receptor alpha interacts with 3-methyladenine DNA glycosylase to modulate transcription and DNA repair, enzyme catalyzes removal of hypoxanthine from DNA, estrogen receptor alpha stabilizes the interaction of the enzyme with hypoxanthine containing DNAand increases the catalytic removal of the modified base Homo sapiens 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O primarily removes N3-methyladenine but also N3-methylguanine from DNA by glycosylic cleavage in the first step of the base excision repair Escherichia coli 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O constitutive pathway for repair of DNA damaged by simple alkylating agents such as methylmethanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine Escherichia coli 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O constititively expressed Escherichia coli 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O constititively expressed Micrococcus luteus 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O initiates the base excision repair pathway by removing damaged bases to create abasic apurinic/apyrimidinic sites Saccharomyces cerevisiae 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O 50% of DNA alkylation is repaired in the first 60 min after treatment with methyl methanesulfonate Homo sapiens 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O highest activity by producing 3-methylguanine Saccharomyces cerevisiae 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O highest activity by producing 3-methylguanine Saccharomyces pombe 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O calf thymus DNA Bacillus subtilis 168 3-methyladenine + ? prefers to release hypoxanthine and 1,N6,ethenoadenine ? 361297 3.2.2.20 alkylated DNA + H2O important role in preventing the mutagenic effects of deaminated purines and cyclic etheno adducts Bacillus subtilis 168 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Escherichia coli B / ATCC 11303 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O constititively expressed Escherichia coli B / ATCC 11303 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Staphylococcus aureus MSSA476 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Escherichia coli BW 9062 3-methyladenine + ? - ? 361297 3.2.2.20 alkylated DNA + H2O - Escherichia coli BW 9062 3-methyladenine + ? also releases a minmal amount of 3-methylguanine ? 361297 3.2.2.20 alkylated DNA + H2O AlkC is specific for 3-methylpurines Bacillus cereus 3-methyladenine + 3-methylguanine + ? - ? 384142 3.2.2.20 alkylated DNA + H2O - Saccharomyces cerevisiae 1,N6-ethenoadenine + ? - ? 430867 3.2.2.20 alkylated DNA + H2O - Saccharomyces pombe 1,N6-ethenoadenine + ? - ? 430867 3.2.2.20 alkylated DNA + H2O - Leptospira interrogans 3-methylguanine + ? - ? 430868 3.2.2.20 alkylated DNA + H2O - Saccharomyces cerevisiae 7-methylguanine + ? - ? 430870 3.2.2.20 alkylated DNA + H2O - Saccharomyces pombe 7-methylguanine + ? - ? 430870 3.2.2.20 alkylated DNA + H2O 50% of DNA alkylation is repaired in the first 60 min after treatment with methyl methanesulfonate Homo sapiens 7-methylguanine + ? - ? 430870 3.2.2.20 alkylated DNA + H2O - Pseudomonas fluorescens 1-methyladenine + ? - ? 446948 3.2.2.20 alkylated DNA + H2O - Bacillus cereus 1-methyladenine + ? - ? 446948 3.2.2.20 alkylated DNA + H2O - Pseudomonas fluorescens 3-methylcytosine + ? - ? 446949 3.2.2.20 alkylated DNA + H2O - Bacillus cereus 3-methylcytosine + ? - ? 446949 3.2.2.20 additional information - Escherichia coli ? - ? 89 3.2.2.20 additional information no activity on O2-methylcytosine, O2-methylthymine, O4-methylthymine or O6-methylguanine Micrococcus luteus ? - ? 89 3.2.2.20 additional information will not release 2,6-diamino-4-hydroxy-5-(N-methylformamido)pyrimidine, the alkali-induced derivative of 7-methylguanine, in which the imidazole ring is opened Escherichia coli ? - ? 89 3.2.2.20 additional information no detectable endonuclease activity on native, depurinated or alkylated plasmid DNA Escherichia coli ? - ? 89 3.2.2.20 additional information does not liberate 7-methylguanine, O6-methylguanine, 7-methyladenine, 7-ethylguanine, O6-ethylguanine, and arylalkylated purine derivatives obtained by treatment of DNA with 7-bromomethyl-12-methylbenz[alpha]anthracene, no detectable nuclease activity with native DNA, depurinated DNA, ultraviolet-irradiated DNA, or X-irradiated DNA as potential substrates, enzyme does not release hypoxanthine or xanthine in free form from by nitrous acid treatment partly demainated DNA Escherichia coli ? - ? 89 3.2.2.20 additional information AlkC is involved exclusively in the repair of alkylation damage Bacillus cereus ? - ? 89 3.2.2.20 additional information no detectable affinity for hypoxanthine, 8-oxoguanine and 5-formyluracil Bacillus cereus ? - ? 89 3.2.2.20 additional information 3-methyladenine DNA glycosylase binds 1,N6-ethenoadenine and abasic sites the most tightly, followed by the cross-linked 1,2-d(ApG) cisplatin adduct in duplex DNA but does not catalyze glycosyl bond cleavage at either of the cross-linked bases Saccharomyces cerevisiae ? - ? 89 3.2.2.20 additional information 3-methyladenine DNA glycosylase recognizes and excises a broad range of purines damaged by alkylation and oxidative damage, including 3-methyladenine, 7-methylguanine, hypoxanthine, and 1,N6-ethenoadenine Homo sapiens ? - ? 89 3.2.2.20 additional information both the full-length and truncated AAG excised 1,N2-ethenoguanine, albeit weakly, from duplex DNA, while uracil is excised from both single- and double-stranded DNA, but only by full-length AAG. Structural basis for substrate recognition, base excision, and exclusion of normal purines and pyrimidines from its substrate recognition pocket, mechanism, overview Homo sapiens ? - ? 89 3.2.2.20 additional information AlkC exhibits robust activity for 3-methylcytosine and modest activity for 1-methyladenosine Pseudomonas fluorescens ? - ? 89 3.2.2.20 additional information AlkC exhibits robust activity for 3-methylcytosine and modest activity for 1-methyladenosine Bacillus cereus ? - ? 89 3.2.2.20 additional information will not release 2,6-diamino-4-hydroxy-5-(N-methylformamido)pyrimidine, the alkali-induced derivative of 7-methylguanine, in which the imidazole ring is opened Escherichia coli BW 9062 ? - ? 89