3.2.1.75 1,6-beta-glucohexaose + H2O - Bacteroides thetaiotaomicron ? - ? 446550 3.2.1.75 1,6-beta-glucohexaose + H2O - Bacteroides thetaiotaomicron VPI-5482 ? - ? 446550 3.2.1.75 1,6-beta-glucooctaose + H2O - Bacteroides thetaiotaomicron ? - ? 446551 3.2.1.75 1,6-beta-glucooctaose + H2O - Bacteroides thetaiotaomicron VPI-5482 ? - ? 446551 3.2.1.75 1,6-beta-glucotetraose + H2O - Bacteroides thetaiotaomicron ? - ? 446552 3.2.1.75 1,6-beta-glucotetraose + H2O - Bacteroides thetaiotaomicron VPI-5482 ? - ? 446552 3.2.1.75 1,6-beta-glucotriose + H2O - Bacteroides thetaiotaomicron ? - ? 446553 3.2.1.75 4-nitrophenyl beta-gentiohexaoside + H2O - Penicillium multicolor ? - ? 431390 3.2.1.75 4-nitrophenyl beta-gentiohexaoside + H2O - Penicillium multicolor LAM7153 ? - ? 431390 3.2.1.75 4-nitrophenyl beta-gentiopentaoside + H2O - Penicillium multicolor ? - ? 431391 3.2.1.75 4-nitrophenyl beta-gentiotetraoside + H2O - Penicillium multicolor ? - ? 431392 3.2.1.75 amylopectin + H2O - Rhizopus microsporus var. chinensis ? - ? 94698 3.2.1.75 amylose + H2O - Neurospora crassa ? - ? 94440 3.2.1.75 amylose + H2O - Rhizopus microsporus var. chinensis ? - ? 94440 3.2.1.75 amylose + H2O - Rhizopus microsporus var. chinensis R-69 ? - ? 94440 3.2.1.75 amylose + H2O - Neurospora crassa IFO-6068 ? - ? 94440 3.2.1.75 beta-(1,6)-glucan + H2O highly branched beta-(1,6)-glucan, that contains about 75-80% of linear beta-(1,6)-glucose linked units, preared from yeast cell walls Trichoderma harzianum ? - ? 431678 3.2.1.75 beta-glucan + H2O Neurospora crassa cell wall beta-glucan Neurospora crassa glucose + laminari-oligosaccharides + gentio-oligosaccharides - ? 94680 3.2.1.75 beta-glucan + H2O yeast glucans Niallia circulans glucose + laminari-oligosaccharides + gentio-oligosaccharides - ? 94680 3.2.1.75 beta-glucan + H2O yeast glucans Acinetobacter sp. glucose + laminari-oligosaccharides + gentio-oligosaccharides - ? 94680 3.2.1.75 beta-glucan + H2O yeast glucans Rhizopus microsporus var. chinensis glucose + laminari-oligosaccharides + gentio-oligosaccharides - ? 94680 3.2.1.75 beta-glucan + H2O yeast glucans Acremonium persicinum glucose + laminari-oligosaccharides + gentio-oligosaccharides - ? 94680 3.2.1.75 beta-glucan + H2O yeast glucans Mucor hiemalis glucose + laminari-oligosaccharides + gentio-oligosaccharides - ? 94680 3.2.1.75 beta-glucan + H2O Saccharomyces cerevisiae glucan Trichoderma harzianum glucose + laminari-oligosaccharides + gentio-oligosaccharides - ? 94680 3.2.1.75 beta-glucan + H2O Ecklonia radiata glucan, poor substrate Penicillium brefeldianum glucose + laminari-oligosaccharides + gentio-oligosaccharides - ? 94680 3.2.1.75 beta-glucan + H2O yeast glucans Niallia circulans WL-12 glucose + laminari-oligosaccharides + gentio-oligosaccharides - ? 94680 3.2.1.75 beta-glucan + H2O Saccharomyces cerevisiae glucan Trichoderma harzianum CECT 2313 glucose + laminari-oligosaccharides + gentio-oligosaccharides - ? 94680 3.2.1.75 beta-glucan + H2O Ecklonia radiata glucan, poor substrate Penicillium brefeldianum QM 1873 glucose + laminari-oligosaccharides + gentio-oligosaccharides - ? 94680 3.2.1.75 carboxymethyl cellulose + H2O - Flavobacterium M64 ? - ? 94663 3.2.1.75 carboxymethyl cellulose + H2O - Mucor hiemalis ? - ? 94663 3.2.1.75 carboxymethyl-pachyman + H2O poor substrate Penicillium brefeldianum ? - ? 94687 3.2.1.75 carboxymethyl-pachyman + H2O poor substrate Penicillium brefeldianum QM 1873 ? - ? 94687 3.2.1.75 carboxymethylcellulose + H2O - Trichoderma harzianum ? - ? 115813 3.2.1.75 cellulose + H2O - Neurospora crassa ? - ? 94577 3.2.1.75 cellulose + H2O - Rhizopus microsporus var. chinensis ? - ? 94577 3.2.1.75 cellulose + H2O - Rhizopus microsporus var. chinensis R-69 ? - ? 94577 3.2.1.75 cellulose + H2O - Neurospora crassa IFO-6068 ? - ? 94577 3.2.1.75 chitosan + H2O 3% of the activity with pustulan Streptomyces sp. EF-14 ? - ? 259769 3.2.1.75 curdlan + H2O 0.3% of the activity against pustulan Streptomyces rochei ? - ? 94323 3.2.1.75 dextran + H2O - Mucor hiemalis ? - ? 94189 3.2.1.75 dextran + H2O low molecular weight Rhizopus microsporus var. chinensis ? - ? 94189 3.2.1.75 dextran + H2O low molecular weight Rhizopus microsporus var. chinensis R-69 ? - ? 94189 3.2.1.75 Eisenia bicyclis laminarin + H2O 27% of the activity with pustulan Acremonium sp. ? - ? 373149 3.2.1.75 gentiobiose + H2O - Flavobacterium M64 D-glucose - ? 94486 3.2.1.75 gentiobiose + H2O poor substrate Penicillium brefeldianum D-glucose - ? 94486 3.2.1.75 gentiobiose + H2O poor substrate Penicillium brefeldianum QM 1873 D-glucose - ? 94486 3.2.1.75 gentiohexaose + H2O - Fusarium fujikuroi gentiobiose + gentiotriose + gentiotetraose - ? 94685 3.2.1.75 gentiohexaose + H2O - Mucor hiemalis gentiobiose + gentiotriose + gentiotetraose - ? 94685 3.2.1.75 gentiooctaose + H2O - Penicillium multicolor ? - ? 430997 3.2.1.75 gentiooctaose + H2O - Penicillium multicolor LAM7153 ? - ? 430997 3.2.1.75 gentiopentaose + H2O - Fusarium fujikuroi D-glucose + gentiotetraose + gentiotriose + gentiobiose - ? 94684 3.2.1.75 gentiopentaose + H2O - Rhizopus microsporus var. chinensis D-glucose + gentiotetraose + gentiotriose + gentiobiose gentiobiose and gentiotriose only ? 94684 3.2.1.75 gentiopentaose + H2O - Mucor hiemalis D-glucose + gentiotetraose + gentiotriose + gentiobiose gentiobiose and gentiotriose only ? 94684 3.2.1.75 gentiopentaose + H2O reduced Acinetobacter sp. D-glucose + gentiotetraose + gentiotriose + gentiobiose - ? 94684 3.2.1.75 gentiopentaosylsorbitol + H2O - Acinetobacter sp. gentiotetraose + gentiobiose + D-glucose - ? 94694 3.2.1.75 gentiotetraose + H2O - Niallia circulans D-glucose + gentiotriose + gentiobiose - ? 94683 3.2.1.75 gentiotetraose + H2O - Fusarium fujikuroi D-glucose + gentiotriose + gentiobiose - ? 94683 3.2.1.75 gentiotetraose + H2O - Rhizopus microsporus var. chinensis D-glucose + gentiotriose + gentiobiose gentiobiose only ? 94683 3.2.1.75 gentiotetraose + H2O - Mucor hiemalis D-glucose + gentiotriose + gentiobiose gentiobiose only ? 94683 3.2.1.75 gentiotetraose + H2O reduced Acinetobacter sp. D-glucose + gentiotriose + gentiobiose - ? 94683 3.2.1.75 gentiotetraose + H2O - Niallia circulans WL-12 D-glucose + gentiotriose + gentiobiose - ? 94683 3.2.1.75 gentiotriose + H2O - Acinetobacter sp. D-glucose + gentiobiose - ? 94682 3.2.1.75 gentiotriosylsorbitol + H2O - Acinetobacter sp. ? - ? 94693 3.2.1.75 glucan + H2O substrate from yeast, 18% of maximal activity Trichoderma harzianum ? - ? 93966 3.2.1.75 glucan + H2O substrate from yeast, 18% of maximal activity Trichoderma harzianum CECT 2413 ? - ? 93966 3.2.1.75 glycogen + H2O - Neurospora crassa ? - ? 94449 3.2.1.75 glycogen + H2O - Rhizopus microsporus var. chinensis ? - ? 94449 3.2.1.75 glycogen + H2O - Rhizopus microsporus var. chinensis R-69 ? - ? 94449 3.2.1.75 glycogen + H2O - Neurospora crassa IFO-6068 ? - ? 94449 3.2.1.75 gyrophoran + H2O - Fusarium fujikuroi ? - ? 94702 3.2.1.75 islandican + H2O - Mucor hiemalis ? - ? 94695 3.2.1.75 lactose + H2O - Rhizopus microsporus var. chinensis D-glucose - ? 94700 3.2.1.75 laminaran + H2O - Neurospora crassa ? - ? 94675 3.2.1.75 laminaran + H2O - Mucor hiemalis ? - ? 94675 3.2.1.75 laminaran + H2O from Eisenia bicyclis Fusarium fujikuroi ? - ? 94675 3.2.1.75 laminarin + H2O 3% activity compared to pustulan Lentinula edodes D-glucose + ? the main product is D-glucose ? 94310 3.2.1.75 laminarin + H2O 3% activity compared to pustulan Lentinula edodes H 600 D-glucose + ? the main product is D-glucose ? 94310 3.2.1.75 laminarin + H2O - Trichoderma harzianum ? - ? 94671 3.2.1.75 laminarin + H2O 0.5% of the activity against pustulan Streptomyces rochei ? - ? 94671 3.2.1.75 laminarin + H2O 12% of the activity with pustulan Neotyphodium sp. ? - ? 94671 3.2.1.75 laminarin + H2O 8% of maximal activity Trichoderma harzianum ? - ? 94671 3.2.1.75 laminarin + H2O only 2% of activity at 45°C Aspergillus fumigatus ? - ? 94671 3.2.1.75 laminarin + H2O 8% of maximal activity Trichoderma harzianum CECT 2413 ? - ? 94671 3.2.1.75 laminarin + H2O 12% of the activity with pustulan Neotyphodium sp. FCB-2002 ? - ? 94671 3.2.1.75 laminarin + H2O - Niallia circulans gentiobiose + D-glucose - ? 94692 3.2.1.75 laminarin + H2O - Acinetobacter sp. gentiobiose + D-glucose - ? 94692 3.2.1.75 laminarin + H2O - Rhizopus microsporus var. chinensis gentiobiose + D-glucose - ? 94692 3.2.1.75 laminarin + H2O - Trichoderma harzianum gentiobiose + D-glucose - ? 94692 3.2.1.75 laminarin + H2O - Acremonium persicinum gentiobiose + D-glucose - ? 94692 3.2.1.75 laminarin + H2O - Saccharomycopsis fibuligera gentiobiose + D-glucose - ? 94692 3.2.1.75 laminarin + H2O - Saccharomycopsis fibuligera NCYC 451 gentiobiose + D-glucose - ? 94692 3.2.1.75 laminarin + H2O - Trichoderma harzianum CECT 2313 gentiobiose + D-glucose - ? 94692 3.2.1.75 laminarin + H2O - Saccharophagus degradans gentobiose + D-glucose - ? 446298 3.2.1.75 laminarin + H2O 22.4% activity compared to pustulan Saccharophagus degradans gentobiose + D-glucose - ? 446298 3.2.1.75 lichenan + H2O - Neurospora crassa ? - ? 94498 3.2.1.75 lichenan + H2O - Neurospora crassa IFO-6068 ? - ? 94498 3.2.1.75 lutean + H2O - Aspergillus niger D-glucose + gentiobiose + gentiotriose - ? 94691 3.2.1.75 lutean + H2O - Acinetobacter sp. D-glucose + gentiobiose + gentiotriose - ? 94691 3.2.1.75 lutean + H2O - Aspergillus phoenicis D-glucose + gentiobiose + gentiotriose - ? 94691 3.2.1.75 lutean + H2O - Fusarium oxysporum D-glucose + gentiobiose + gentiotriose - ? 94691 3.2.1.75 lutean + H2O - Aspergillus usamii D-glucose + gentiobiose + gentiotriose - ? 94691 3.2.1.75 lutean + H2O - Acremonium persicinum D-glucose + gentiobiose + gentiotriose - ? 94691 3.2.1.75 lutean + H2O - Fusarium fujikuroi D-glucose + gentiobiose + gentiotriose - ? 94691 3.2.1.75 lutean + H2O - Helminthosporium sesanum D-glucose + gentiobiose + gentiotriose - ? 94691 3.2.1.75 lutean + H2O - Alternaria bataticola D-glucose + gentiobiose + gentiotriose - ? 94691 3.2.1.75 lutean + H2O 73% of the activity with pustulan Acremonium sp. ? - ? 373718 3.2.1.75 luteose + H2O - Rhizopus microsporus var. chinensis gentiobiose + gentiotriose + gentiotetraose - ? 94697 3.2.1.75 luteose + H2O - Flavobacterium M64 gentiobiose + gentiotriose + gentiotetraose - ? 94697 3.2.1.75 methyl beta-gentiobioside + H2O low activity Penicillium multicolor methyl beta-D-glucoside + beta-D-glucose - ? 432123 3.2.1.75 methyl beta-gentiohexaoside + H2O the major cleavages occur at bonds 2, 3, and 4 in approximately equal proportion, the nonreducing end glucosyl residues of Gen4-6 beta-OMe are either not or scarcely cleaved by the enzyme Penicillium multicolor methyl beta-D-glucoside + 2 beta-D-glucose + gentiobiose - ? 432124 3.2.1.75 methyl beta-gentiopentaoside + H2O the major cleavages occur at bonds 2, 3, and 4 in approximately equal proportion Penicillium multicolor methyl beta-D-glucoside + 3 beta-D-glucose - ? 432125 3.2.1.75 methyl beta-gentiotetraoside + H2O cleavage at the second (bond 2) and third (bond 3) glucosidic bonds from the methyl group Penicillium multicolor methyl beta-D-glucoside + 2 beta-D-glucose - ? 432126 3.2.1.75 methyl beta-gentiotrioside + H2O the second glucosidic bond (bond 2) from the methyl group is cut preferentially Penicillium multicolor methyl beta-D-glucoside + gentiobiose Glc beta-OMe and Gen2 beta-OMe in the ratio 91:9 ? 432127 3.2.1.75 additional information - Fusarium fujikuroi ? - ? 89 3.2.1.75 additional information little activity against beta-(1-3)- or beta-(1-4)-linkages Streptomyces rochei ? - ? 89 3.2.1.75 additional information no substrate: laminarin Streptomyces sp. EF-14 ? - ? 89 3.2.1.75 additional information BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview Trichoderma harzianum ? - ? 89 3.2.1.75 additional information substrate specificity, BGN16.3 shows endohydrolytic activity against substrates with beta-1,6-glycosidic linkages, no activity with colloidal chitin, pachyman, starch, cellulose, nigeran, and dextran, no activity with the beta-1,6-disaccharide gentiobiose Trichoderma harzianum ? - ? 89 3.2.1.75 additional information is active on Candida albicans cell walls, is able to release a GPI-anchored cell wall protein linked to the beta-1,6-glucan polymer through a remnant of its anchor. No activity with beta-1,3-glucan, dextran and amylose Aspergillus fumigatus ? - ? 89 3.2.1.75 additional information no activity is detected when gentiobiose, lentinan, laminarioligosaccharides, pachyman, CM-pachyman, and lichenan are used as substrates Lentinula edodes ? - ? 89 3.2.1.75 additional information the purified endo-beta-1,6-glucanase Pus30A catalyzes depolymerization of beta-1,6-glucan endolytically and is highly specific toward beta-1,6-glucan polysaccharide. Pus30A shows hydrolyzing activity against the cell wall components of Lentinula edodes fruiting bodies (54.5% activity compared to pustulan) Lentinula edodes ? - ? 89 3.2.1.75 additional information no activity with laminarin, chitin, and mannan Trichoderma harzianum ? - ? 89 3.2.1.75 additional information the enzyme is a specific beta-(1->6)-glucanase, it shows no activity with soluble starch, carboxymethyl cellulose, curdlan from Alcaligenes faecalis, and 4-nitrophenyl beta-gentiobioside, it shows poor activity with yeast glucan and 4-nitrophenyl beta-gentiotrioside. The cleavage point of 4-nitrophenyl oligosacchride derivatives with DP 4 or higher shifts such that the enzyme keeps away from the 4-nitrophenyl group at the terminus, in all the tested substrates, release of 4-nitrophenol is not observed. Gentiooligosaccharides (DP 3-9) are prepared from the starting substance gentiobiose by the sequential transglycosylation reactions of beta-glucosidase and beta-(1->6)-glucanase Penicillium multicolor ? - ? 89 3.2.1.75 additional information no activity with curdlan, barley beta-glucan, xylan and carboxymethyl cellulose Saccharophagus degradans ? - ? 89 3.2.1.75 additional information the enzyme shows no activity on gentiobiose, 1,3-beta-glucan, 1,4-beta-glucan, or 1,6-beta-galactan Bacteroides thetaiotaomicron ? - ? 89 3.2.1.75 additional information no activity is detected when gentiobiose, lentinan, laminarioligosaccharides, pachyman, CM-pachyman, and lichenan are used as substrates Lentinula edodes H 600 ? - ? 89 3.2.1.75 additional information the purified endo-beta-1,6-glucanase Pus30A catalyzes depolymerization of beta-1,6-glucan endolytically and is highly specific toward beta-1,6-glucan polysaccharide. Pus30A shows hydrolyzing activity against the cell wall components of Lentinula edodes fruiting bodies (54.5% activity compared to pustulan) Lentinula edodes H 600 ? - ? 89 3.2.1.75 additional information BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview Trichoderma harzianum CECT 2413 ? - ? 89 3.2.1.75 additional information substrate specificity, BGN16.3 shows endohydrolytic activity against substrates with beta-1,6-glycosidic linkages, no activity with colloidal chitin, pachyman, starch, cellulose, nigeran, and dextran, no activity with the beta-1,6-disaccharide gentiobiose Trichoderma harzianum CECT 2413 ? - ? 89 3.2.1.75 additional information the enzyme shows no activity on gentiobiose, 1,3-beta-glucan, 1,4-beta-glucan, or 1,6-beta-galactan Bacteroides thetaiotaomicron VPI-5482 ? - ? 89 3.2.1.75 additional information the enzyme is a specific beta-(1->6)-glucanase, it shows no activity with soluble starch, carboxymethyl cellulose, curdlan from Alcaligenes faecalis, and 4-nitrophenyl beta-gentiobioside, it shows poor activity with yeast glucan and 4-nitrophenyl beta-gentiotrioside. The cleavage point of 4-nitrophenyl oligosacchride derivatives with DP 4 or higher shifts such that the enzyme keeps away from the 4-nitrophenyl group at the terminus, in all the tested substrates, release of 4-nitrophenol is not observed. Gentiooligosaccharides (DP 3-9) are prepared from the starting substance gentiobiose by the sequential transglycosylation reactions of beta-glucosidase and beta-(1->6)-glucanase Penicillium multicolor LAM7153 ? - ? 89 3.2.1.75 p-nitrophenyl-beta-D-glucoside + H2O - Niallia circulans 4-nitrophenol + beta-D-glucose - ? 94690 3.2.1.75 p-nitrophenyl-beta-D-glucoside + H2O - Flavobacterium M64 4-nitrophenol + beta-D-glucose - ? 94690 3.2.1.75 p-nitrophenyl-beta-D-glucoside + H2O - Niallia circulans WL-12 4-nitrophenol + beta-D-glucose - ? 94690 3.2.1.75 pachyman + H2O - Rhizopus microsporus var. chinensis gentiobiose - ? 94686 3.2.1.75 pachyman + H2O poor substrate Penicillium brefeldianum gentiobiose - ? 94686 3.2.1.75 pachyman + H2O poor substrate Niallia circulans gentiobiose trace ? 94686 3.2.1.75 pachyman + H2O poor substrate Niallia circulans WL-12 gentiobiose trace ? 94686 3.2.1.75 pachyman + H2O poor substrate Penicillium brefeldianum QM 1873 gentiobiose - ? 94686 3.2.1.75 pustulan + H2O - Trichoderma harzianum ? - ? 94320 3.2.1.75 pustulan + H2O - Aspergillus fumigatus ? - ? 94320 3.2.1.75 pustulan + H2O - Penicillium multicolor ? - ? 94320 3.2.1.75 pustulan + H2O - Acremonium sp. ? - ? 94320 3.2.1.75 pustulan + H2O - Neotyphodium sp. ? - ? 94320 3.2.1.75 pustulan + H2O high substrate specificity, 100% activity Lentinula edodes ? - ? 94320 3.2.1.75 pustulan + H2O from Umbilicaria pustulata Trichoderma harzianum ? - ? 94320 3.2.1.75 pustulan + H2O the enzyme completely hydrolyzes pustulan in three steps: in the initial stage, the enzyme quickly cleaves the glucan with a pattern resembling an endo-hydrolase to produce a short-chain glucan (degree of polymerization DP 45) as an intermediate. In the midterm stage, the resulting short-chain glucan is further cleaved into two fractions corresponding to DP 15-7 and DP 2-4 with great regularity. In the final stage, the lower oligomers corresponding to DP 3 and DP 4 are very slowly hydrolyzed into glucose and gentiobiose Penicillium multicolor ? - ? 94320 3.2.1.75 pustulan + H2O high substrate specificity, 100% activity Lentinula edodes H 600 ? - ? 94320 3.2.1.75 pustulan + H2O - Neotyphodium sp. FCB-2002 ? - ? 94320 3.2.1.75 pustulan + H2O - Penicillium multicolor LAM7153 ? - ? 94320 3.2.1.75 pustulan + H2O the enzyme completely hydrolyzes pustulan in three steps: in the initial stage, the enzyme quickly cleaves the glucan with a pattern resembling an endo-hydrolase to produce a short-chain glucan (degree of polymerization DP 45) as an intermediate. In the midterm stage, the resulting short-chain glucan is further cleaved into two fractions corresponding to DP 15-7 and DP 2-4 with great regularity. In the final stage, the lower oligomers corresponding to DP 3 and DP 4 are very slowly hydrolyzed into glucose and gentiobiose Penicillium multicolor LAM7153 ? - ? 94320 3.2.1.75 pustulan + H2O - Neurospora crassa glucose + gentiobiose - ? 94674 3.2.1.75 pustulan + H2O - Niallia circulans glucose + gentiobiose - ? 94674 3.2.1.75 pustulan + H2O - Trichoderma harzianum glucose + gentiobiose - ? 94674 3.2.1.75 pustulan + H2O - Acremonium persicinum glucose + gentiobiose - ? 94674 3.2.1.75 pustulan + H2O - Trichoderma sp. glucose + gentiobiose - ? 94674 3.2.1.75 pustulan + H2O - Chlamys albidus glucose + gentiobiose - ? 94674 3.2.1.75 pustulan + H2O - Niallia circulans glucose + gentiobiose also gentiopentaose, gentiotetraose and gentiotriose ? 94674 3.2.1.75 pustulan + H2O - Chlamys albidus glucose + gentiobiose glucose, gentiobiose and gentiotriose ? 94674 3.2.1.75 pustulan + H2O - Penicillium sp. glucose + gentiobiose gentiotetraose and longer chains, gentiotriose, gentiotriose and glucose ? 94674 3.2.1.75 pustulan + H2O - Trichoderma harzianum glucose + gentiobiose glucose, gentiotetraose, gentiobiose ? 94674 3.2.1.75 pustulan + H2O - Acinetobacter sp. glucose + gentiobiose glucose and oligosaccharides ? 94674 3.2.1.75 pustulan + H2O - Niallia circulans WL-12 glucose + gentiobiose also gentiopentaose, gentiotetraose and gentiotriose ? 94674 3.2.1.75 pustulan + H2O - Niallia circulans WL-12 glucose + gentiobiose - ? 94674 3.2.1.75 pustulan + H2O - Neurospora crassa IFO-6068 glucose + gentiobiose - ? 94674 3.2.1.75 pustulan + H2O - Trichoderma harzianum CECT 2313 glucose + gentiobiose glucose, gentiotetraose, gentiobiose ? 94674 3.2.1.75 pustulan + H2O - Streptomyces rochei D-glucose + gentobiose + ? main products ? 374465 3.2.1.75 pustulan + H2O - Streptomyces sp. EF-14 gentobiose + D-glucose + ? - ? 374466 3.2.1.75 pustulan + H2O best substrate Trichoderma harzianum oligomers of pustulan product analysis, most abundant products are di-, tri-, and tetra-beta-1,6-glucosides ? 382691 3.2.1.75 pustulan + H2O best substrate Trichoderma harzianum CECT 2413 oligomers of pustulan product analysis, most abundant products are di-, tri-, and tetra-beta-1,6-glucosides ? 382691 3.2.1.75 pustulan + H2O - Saccharophagus degradans gentobiose + D-glucose - ? 446340 3.2.1.75 pustulan + H2O 100% activity Saccharophagus degradans gentobiose + D-glucose - ? 446340 3.2.1.75 pustulan + H2O highly specific substrate Bacteroides thetaiotaomicron gentobiose + D-glucose - ? 446340 3.2.1.75 pustulan + H2O highly specific substrate Bacteroides thetaiotaomicron VPI-5482 gentobiose + D-glucose - ? 446340 3.2.1.75 starch + H2O soluble, from corn, from potato, from wheat Rhizopus microsporus var. chinensis ? - ? 94621 3.2.1.75 starch + H2O 8% of the activity with pustulan Streptomyces sp. EF-14 ? - ? 94621 3.2.1.75 starch + H2O soluble, from corn, from potato, from wheat Rhizopus microsporus var. chinensis R-69 ? - ? 94621 3.2.1.75 succinoglycan + H2O - Flavobacterium M64 ? - ? 94688 3.2.1.75 xylan + H2O 12% of the activity with pustulan Streptomyces sp. EF-14 ? - ? 94170