3.2.1.68 alpha-D-glucan + H2O cleavage of alpha-D-glucosidic linkages of certain branched alpha-D-glucans Pseudomonas amyloderamosa ? - ? 94607 3.2.1.68 alpha-limit dextrin + H2O - Myroides odoratus linear maltooligosaccharides - ? 416560 3.2.1.68 alpha-limit dextrin + H2O - Pseudomonas amyloderamosa linear maltooligosaccharides - ? 416560 3.2.1.68 alpha-limit dextrin + H2O - Pseudomonas amyloderamosa SB-15 linear maltooligosaccharides - ? 416560 3.2.1.68 amylomaize + H2O debranching Pseudomonas sp. ? - ? 431591 3.2.1.68 amylopectin + H2O - Oryza sativa maltose + maltooligosaccharides - ? 94609 3.2.1.68 amylopectin + H2O - Oryza sativa maltose + maltooligosaccharides - r 94609 3.2.1.68 amylopectin + H2O waxy maize amylopectin, potato amylopectin, amylopectin beta-dextrin Pseudomonas amyloderamosa maltose + maltooligosaccharides - ? 94609 3.2.1.68 amylopectin + H2O specific cleavage of alpha 1,6-glucosidic inter-chain linkages producing essentially linear chains Bacillus sp. (in: Bacteria) maltose + maltooligosaccharides - ? 94609 3.2.1.68 amylopectin + H2O or the beta-limit dextrins, cleaves the branching points completely in vitro Zea mays maltose + maltooligosaccharides - r 94609 3.2.1.68 amylopectin + H2O or the beta-limit dextrins, cleaves the branching points completely in vitro Bacillus sp. (in: Bacteria) maltose + maltooligosaccharides - ? 94609 3.2.1.68 amylopectin + H2O or the beta-limit dextrins, cleaves the branching points completely in vitro Lipomyces kononenkoae maltose + maltooligosaccharides - ? 94609 3.2.1.68 amylopectin + H2O or the beta-limit dextrins, cleaves the branching points completely in vitro Pseudomonas amyloderamosa maltose + maltooligosaccharides - ? 94609 3.2.1.68 amylopectin + H2O or the beta-limit dextrins, cleaves the branching points completely in vitro Solanum tuberosum maltose + maltooligosaccharides hydrolysis of beta-limit dextrins forming maltotriose as main reaction product ? 94609 3.2.1.68 amylopectin + H2O or the beta-limit dextrins, cleaves the branching points completely in vitro Pseudomonas amyloderamosa maltose + maltooligosaccharides hydrolysis of beta-limit dextrins forming maltotriose as main reaction product ? 94609 3.2.1.68 amylopectin + H2O isoamylase is a direct debranching enzyme with the ability to cleave all the alpha-1,6 linkages of glycogen both inner and outer branching points of soluble amylopectin Myroides odoratus maltose + maltooligosaccharides - ? 94609 3.2.1.68 amylopectin + H2O isoamylase is a direct debranching enzyme with the ability to cleave all the alpha-1,6 linkages of glycogen both inner and outer branching points of soluble amylopectin Pseudomonas amyloderamosa maltose + maltooligosaccharides - ? 94609 3.2.1.68 amylopectin + H2O or the beta-limit dextrins, cleaves the branching points completely in vitro Pseudomonas amyloderamosa SMP1 maltose + maltooligosaccharides - ? 94609 3.2.1.68 amylopectin + H2O isoamylase is a direct debranching enzyme with the ability to cleave all the alpha-1,6 linkages of glycogen both inner and outer branching points of soluble amylopectin Pseudomonas amyloderamosa SB-15 maltose + maltooligosaccharides - ? 94609 3.2.1.68 amylopectin + H2O - Chlamydomonas reinhardtii ? - ? 94698 3.2.1.68 amylopectin + H2O - Oryza sativa ? - ? 94698 3.2.1.68 amylopectin + H2O - Thermobifida fusca ? - ? 94698 3.2.1.68 amylopectin + H2O - Solanum tuberosum ? - ? 94698 3.2.1.68 amylopectin + H2O - Arabidopsis thaliana ? - ? 94698 3.2.1.68 amylopectin + H2O - Oryza sativa Japonica Group ? - ? 94698 3.2.1.68 amylopectin + H2O - Zea mays ? - ? 94698 3.2.1.68 amylopectin + H2O - Ipomoea batatas ? - ? 94698 3.2.1.68 amylopectin + H2O - Dickeya chrysanthemi ? hydrolysis of alpha-1,6-linkages ? 94698 3.2.1.68 amylopectin + H2O isoform ISA1 removes the wide range of chains with a degree of polymerization (DP) of 6-20 from amylopectin although the long chains of DP higher than 20 are debranched by the prolonged incubation. Isoform ISA1 shows 356% activity compared to phosphorylase a limit dextrin. Isoform ISA3 shows trace activity compared to phosphorylase a limit dextrin Oryza sativa ? - ? 94698 3.2.1.68 amylopectin + H2O the enzyme can debranch almost all of component chains of amylopectin Pseudomonas amyloderamosa ? - ? 94698 3.2.1.68 amylopectin + H2O the enzyme can debranch almost all of component chains of amylopectin and shows 9.4% activity compared to phosphorylase a limit dextrin Crocosphaera subtropica ATCC 51142 ? - ? 94698 3.2.1.68 amylopectin + H2O the enzyme shows 0.8% activity compared to phosphorylase a limit dextrin Escherichia coli ? - ? 94698 3.2.1.68 amylopectin + H2O - Chlamydomonas reinhardtii 330 ? - ? 94698 3.2.1.68 amylopectin + H2O - Arabidopsis thaliana Col-0 ? - ? 94698 3.2.1.68 amylopectin + H2O - Dickeya chrysanthemi PY35 ? hydrolysis of alpha-1,6-linkages ? 94698 3.2.1.68 amylopectin + H2O - Saccharolobus solfataricus linear maltooligosaccharides - ? 391280 3.2.1.68 amylopectin + H2O - Pseudomonas amyloderamosa linear maltooligosaccharides - ? 391280 3.2.1.68 amylopectin + H2O - Phaseolus vulgaris linear maltooligosaccharides - ? 391280 3.2.1.68 amylopectin + H2O - Bacillus sp. (in: Bacteria) amylose + linear maltooligosaccharides - ? 430879 3.2.1.68 amylopectin + H2O - Bacillus sp. (in: Bacteria) CICIM 304 amylose + linear maltooligosaccharides - ? 430879 3.2.1.68 amylopectin phi-dextrin + H2O substrate on which all 3 types of debranching enzymes act Pseudomonas amyloderamosa maltotetraose - ? 94610 3.2.1.68 amylose + H2O short chain Solanum tuberosum ? - ? 94440 3.2.1.68 amylose-extender amylopectin + H2O - Oryza sativa maltose + maltooligosaccharides - ? 391283 3.2.1.68 Arabidopsis starch + H2O - Arabidopsis thaliana ? - ? 430895 3.2.1.68 Arabidopsis starch + H2O - Arabidopsis thaliana Col-0 ? - ? 430895 3.2.1.68 beta limit dextrin + H2O - Phaseolus vulgaris linear maltooligosaccharides - ? 391474 3.2.1.68 beta limit dextrin + H2O from maize Oryza sativa maltose + maltooligosaccharides - ? 391475 3.2.1.68 beta-amylase limit dextrin + H2O isoform ISA1 shows 71% activity compared to phosphorylase a limit dextrin. Isoform ISA3 shows 48% activity compared to phosphorylase a limit dextrin Oryza sativa ? - ? 447198 3.2.1.68 beta-amylase limit dextrin + H2O the enzyme shows 34% activity compared to phosphorylase a limit dextrin Escherichia coli ? - ? 447198 3.2.1.68 beta-amylase limit dextrin + H2O the enzyme shows 55% activity compared to phosphorylase a limit dextrin Crocosphaera subtropica ATCC 51142 ? - ? 447198 3.2.1.68 beta-limit dextrin + H2O - Oryza sativa ? - ? 259724 3.2.1.68 beta-limit dextrin + H2O - Thermobifida fusca ? - ? 259724 3.2.1.68 beta-limit dextrin + H2O - Solanum tuberosum ? - ? 259724 3.2.1.68 beta-limit dextrin + H2O - Arabidopsis thaliana ? - ? 259724 3.2.1.68 beta-limit dextrin + H2O - Chlamydomonas reinhardtii ? - ? 259724 3.2.1.68 beta-limit dextrin + H2O - Zea mays ? - ? 259724 3.2.1.68 beta-limit dextrin + H2O best substrate Solanum tuberosum ? - ? 259724 3.2.1.68 beta-limit dextrin + H2O medium activity Solanum tuberosum ? - ? 259724 3.2.1.68 beta-limit dextrin + H2O - Chlamydomonas reinhardtii 330 ? - ? 259724 3.2.1.68 beta-limit dextrin + H2O - Arabidopsis thaliana Col-0 ? - ? 259724 3.2.1.68 beta-limit dextrin + H2O - Musa acuminata maltose + ? - ? 381582 3.2.1.68 beta-limit dextrin + H2O - Myroides odoratus linear maltooligosaccharides - ? 416723 3.2.1.68 beta-limit dextrin + H2O - Pseudomonas amyloderamosa linear maltooligosaccharides - ? 416723 3.2.1.68 beta-limit dextrin + H2O - Pseudomonas amyloderamosa SB-15 linear maltooligosaccharides - ? 416723 3.2.1.68 branched chain oligosaccharides + H2O - Pseudomonas amyloderamosa maltotriose + higher maltosaccharides no detectable maltose released, characteristic action is hydrolysis of alpha 1,6-glucosidic linkages, but alpha 1,6 glucosidic linkages attaching maltose residues to chains of alpha 1,4-linked glucose residues are not readily hydrolyzed ? 94611 3.2.1.68 corn amylopectin + H2O - Pseudomonas amyloderamosa ? - ? 411896 3.2.1.68 corn amylopectin + H2O - Pseudomonas amyloderamosa WU7211-2 ? - ? 411896 3.2.1.68 D-glucose + H2O 59% of the activity with dextrin as substrate Lipomyces kononenkoae ? - ? 94613 3.2.1.68 dextrin + H2O enzyme activity 100% Lipomyces kononenkoae ? - ? 93969 3.2.1.68 di-O-alpha-maltosyl-beta-cyclodextrin + H2O - Pseudomonas amyloderamosa maltose + beta-cyclodextrin - r 391824 3.2.1.68 ginkgo starch + H2O 74.74% yield at pH 5.0 and 52°C after 170 min Pseudomonas sp. ginkgo amylose - ? 447556 3.2.1.68 glycogen + H2O - Thermobifida fusca ? - ? 94449 3.2.1.68 glycogen + H2O - Lederbergia lentus ? - ? 94449 3.2.1.68 glycogen + H2O - Chlamydomonas reinhardtii ? - ? 94449 3.2.1.68 glycogen + H2O - Bacillus sp. (in: Bacteria) ? - ? 94449 3.2.1.68 glycogen + H2O 80% activity compared to amylopectin Dickeya chrysanthemi ? - ? 94449 3.2.1.68 glycogen + H2O - Chlamydomonas reinhardtii 330 ? - ? 94449 3.2.1.68 glycogen + H2O - Bacillus sp. (in: Bacteria) CICIM 304 ? - ? 94449 3.2.1.68 glycogen + H2O 80% activity compared to amylopectin Dickeya chrysanthemi PY35 ? - ? 94449 3.2.1.68 glycogen + H2O - Lederbergia lentus JNU3 ? - ? 94449 3.2.1.68 glycogen + H2O - Oryza sativa maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O - Oryza sativa maltose + maltooligosaccharides - r 94614 3.2.1.68 glycogen + H2O - Musa acuminata maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O - Myroides odoratus maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O - Zea mays maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O - Pseudomonas amyloderamosa maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O cleaves the branching points completely in vitro Zea mays maltose + maltooligosaccharides - r 94614 3.2.1.68 glycogen + H2O cleaves the branching points completely in vitro Solanum tuberosum maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O cleaves the branching points completely in vitro Bacillus sp. (in: Bacteria) maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O cleaves the branching points completely in vitro Lipomyces kononenkoae maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O cleaves the branching points completely in vitro Pseudomonas amyloderamosa maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O oyster glycogen, glycogen beta-dextrin, rabbit liver glycogen Pseudomonas amyloderamosa maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O or the beta-limit dextrins Zea mays maltose + maltooligosaccharides - r 94614 3.2.1.68 glycogen + H2O or the beta-limit dextrins Solanum tuberosum maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O or the beta-limit dextrins Bacillus sp. (in: Bacteria) maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O or the beta-limit dextrins Lipomyces kononenkoae maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O or the beta-limit dextrins Pseudomonas amyloderamosa maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O cleaves the branching points completely in vitro Pseudomonas amyloderamosa SMP1 maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O or the beta-limit dextrins Pseudomonas amyloderamosa SMP1 maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O - Pseudomonas amyloderamosa SB-15 maltose + maltooligosaccharides - ? 94614 3.2.1.68 glycogen + H2O - Saccharolobus solfataricus linear maltooligosaccharides - ? 392143 3.2.1.68 glycogen + H2O - Phaseolus vulgaris linear maltooligosaccharides - ? 392143 3.2.1.68 glycogen + H2O - Arthrobacter sp. maltodextrin - ? 417955 3.2.1.68 glycogen + H2O - Arthrobacter sp. Q36 maltodextrin - ? 417955 3.2.1.68 maize amylopectin + H2O isoamylase hydrolyses both inner and outer branching linkages of amylopectin simultaneously Lederbergia lentus ? - ? 447720 3.2.1.68 maize beta-limit dextrin + H2O - Arabidopsis thaliana ? - ? 432101 3.2.1.68 maize starch + H2O - Lederbergia lentus ? - ? 431059 3.2.1.68 maize starch + H2O - Bacillus sp. (in: Bacteria) ? - ? 431059 3.2.1.68 maize starch + H2O - Zea mays ? - ? 431059 3.2.1.68 maltodextrin + H2O - Bacillus sp. (in: Bacteria) ? - ? 259754 3.2.1.68 maltodextrin + H2O - Bacillus sp. (in: Bacteria) CICIM 304 ? - ? 259754 3.2.1.68 maltopentaose + H2O sugar immobilized on Sepharose 4B Solanum tuberosum ? - ? 94184 3.2.1.68 maltose + H2O - Pseudomonas amyloderamosa ? - ? 94579 3.2.1.68 maltose + H2O iam gene induced by maltose Flavobacterium sp. ? - ? 94579 3.2.1.68 maltose + H2O iam gene induced by maltose Pseudomonas amyloderamosa ? - ? 94579 3.2.1.68 maltose + H2O iam gene induced by maltose Pseudomonas amyloderamosa JD210 ? - ? 94579 3.2.1.68 maltose + H2O iam gene induced by maltose Pseudomonas amyloderamosa SMP1 ? - ? 94579 3.2.1.68 maltose + H2O iam gene induced by maltose Pseudomonas amyloderamosa MI-414 ? - ? 94579 3.2.1.68 maltotriose + H2O activity 28% Lipomyces kononenkoae ? - ? 94410 3.2.1.68 additional information direct debranching enzyme, attacks unmodified glycogen and/or amylopectin with hydrolysis of the 1,6-bond, smallest substrate is not known Solanum tuberosum ? - ? 89 3.2.1.68 additional information direct debranching enzyme, attacks unmodified glycogen and/or amylopectin with hydrolysis of the 1,6-bond, smallest substrate is not known Pseudomonas amyloderamosa ? - ? 89 3.2.1.68 additional information distinguished from alpha-dextrin endo-1,6-alpha-glucosidase, EC 3.2.1.41, by the inability of isoamylase to attack pullulan, and by limited action on alpha-limit dextrins, action on glycogen however is complete in contrast to limited action by alpha-dextrin glucanohydrolase, 1,6-linkage hydrolysed only at branch point Solanum tuberosum ? - ? 89 3.2.1.68 additional information distinguished from alpha-dextrin endo-1,6-alpha-glucosidase, EC 3.2.1.41, by the inability of isoamylase to attack pullulan, and by limited action on alpha-limit dextrins, action on glycogen however is complete in contrast to limited action by alpha-dextrin glucanohydrolase, 1,6-linkage hydrolysed only at branch point Pseudomonas amyloderamosa ? - ? 89 3.2.1.68 additional information no activity with pullulan Musa acuminata ? - ? 89 3.2.1.68 additional information involved in starch synthesis Solanum tuberosum ? - ? 89 3.2.1.68 additional information involved in synthesis of reserve and leaf starches Triticum aestivum ? - ? 89 3.2.1.68 additional information no substrate: dextran, amylose Dickeya chrysanthemi ? - ? 89 3.2.1.68 additional information no substrate: pullulan, glycogen Chlamydomonas reinhardtii ? - ? 89 3.2.1.68 additional information AtISA1 and AtISA2 are required for the production of a functional isoamylase-type of debranching enzyme named Iso1, the major isoamylase found in leaves. The absence of Iso1 leads to an 80% decrease in the starch content and to accumulation of water-soluble polysaccharides whose structure is similar to glycogen Arabidopsis thaliana ? - ? 89 3.2.1.68 additional information AtISA1/AtISA2 isoamylase influences glucan branching pattern Arabidopsis thaliana ? - ? 89 3.2.1.68 additional information glucan debranching occurs primarily at the granule surface via ISA3, but in its absence soluble branched glucans are debranched in the stroma via limit dextrinase. Isoamylase acts at the surface of the starch granule. Atisa3 mutants have more leaf starch and a slower rate of starch breakdown than wild-type plants Arabidopsis thaliana ? - ? 89 3.2.1.68 additional information ISA1 activity plays a critical role in the stochastic process in starch synthesis in rice endosperm Oryza sativa ? - ? 89 3.2.1.68 additional information isoamylase1 is directly involved in the synthesis of amylopectin Triticum aestivum ? - ? 89 3.2.1.68 additional information mutant lines carrying a defect in AtISA3 display a strong starch-excess phenotype at the end of both the light and the dark phases accompanied by a small modification of the amylopectin structure Arabidopsis thaliana ? - ? 89 3.2.1.68 additional information TreX from Sulfolobus solfataricus shows dual activities for alpha-1,4-transferase, EC 2.4.1.25 and alpha-1,6-glucosidase, EC 3.2.1.68, bifunctional mechanism, substrate glycogen, overview. TreX exhibits two different active-site configurations depending on its oligomeric state Saccharolobus solfataricus ? - ? 89 3.2.1.68 additional information isoamylase is one of the starch-debranching enzymes which catalyzes the hydrolysis of alpha-1,6-glucosidic linkages specific in alpha-glucans including amylopectin, branched starch, and glycogen Pseudomonas amyloderamosa ? - ? 89 3.2.1.68 additional information isoform Psisa2 most likely does not have catalytic activity Pisum sativum ? - ? 89 3.2.1.68 additional information no activity towards pullulan Myroides odoratus ? - ? 89 3.2.1.68 additional information no activity towards pullulan Pseudomonas amyloderamosa ? - ? 89 3.2.1.68 additional information recombinant isoamylase 3 does not readily hydrolyze red pullulan and amylose Oryza sativa ? - ? 89 3.2.1.68 additional information isoamylase catalyzes the hydrolysis of alpha-1,6-glucosidic linkage specifically in amylopectin, glycogen and derived oligosaccharides Arthrobacter sp. ? - ? 89 3.2.1.68 additional information synthesis of amylopectin and phytoglycogen by associated and separated ISA1 and ISA2, respectively, overview Arabidopsis thaliana ? - ? 89 3.2.1.68 additional information Zea mays subunit ISA 2 alone is catalytically inactive Zea mays ? - ? 89 3.2.1.68 additional information pullulan is a poor substrate Chlamydomonas reinhardtii ? - ? 89 3.2.1.68 additional information pullulan is a poor substrate Bacillus sp. (in: Bacteria) ? - ? 89 3.2.1.68 additional information no activity with amylose Lederbergia lentus ? - ? 89 3.2.1.68 additional information pullulan is a poor substrate Chlamydomonas reinhardtii 330 ? - ? 89 3.2.1.68 additional information pullulan is a poor substrate Bacillus sp. (in: Bacteria) CICIM 304 ? - ? 89 3.2.1.68 additional information isoamylase is one of the starch-debranching enzymes which catalyzes the hydrolysis of alpha-1,6-glucosidic linkages specific in alpha-glucans including amylopectin, branched starch, and glycogen Pseudomonas amyloderamosa WU7211-2 ? - ? 89 3.2.1.68 additional information no substrate: dextran, amylose Dickeya chrysanthemi PY35 ? - ? 89 3.2.1.68 additional information isoamylase catalyzes the hydrolysis of alpha-1,6-glucosidic linkage specifically in amylopectin, glycogen and derived oligosaccharides Arthrobacter sp. Q36 ? - ? 89 3.2.1.68 additional information no activity towards pullulan Pseudomonas amyloderamosa SB-15 ? - ? 89 3.2.1.68 oyster glycogen + H2O - Oryza sativa ? - ? 407281 3.2.1.68 oyster glycogen + H2O - Arabidopsis thaliana ? - ? 407281 3.2.1.68 oyster glycogen + H2O isoform ISA1 shows 104% activity compared to phosphorylase a limit dextrin. Isoform ISA3 shows 1.5% activity compared to phosphorylase a limit dextrin Oryza sativa ? - ? 407281 3.2.1.68 oyster glycogen + H2O the enzyme shows 14% activity compared to phosphorylase a limit dextrin Crocosphaera subtropica ATCC 51142 ? - ? 407281 3.2.1.68 oyster glycogen + H2O the enzyme shows 2% activity compared to phosphorylase a limit dextrin Escherichia coli ? - ? 407281 3.2.1.68 oyster gylcogen + H2O - Oryza sativa maltose + maltooligosaccharides - ? 393061 3.2.1.68 panose + H2O acts very slowly, small amounts of product formed after 24 h Lipomyces kononenkoae D-glucose + maltose - ? 94618 3.2.1.68 phosphorylase a limit dextrin + H2O 100% activity Escherichia coli ? - ? 447988 3.2.1.68 phosphorylase a limit dextrin + H2O 100% activity Oryza sativa ? - ? 447988 3.2.1.68 phosphorylase a limit dextrin + H2O 100% activity Crocosphaera subtropica ATCC 51142 ? - ? 447988 3.2.1.68 phythoglycogen + H2O - Zea mays ? - r 94619 3.2.1.68 phythoglycogen + H2O - Oryza sativa ? - r 94619 3.2.1.68 phythoglycogen + H2O highly branched polysaccharide, complete hydrolysis of the alpha-1,6-glucosidic bonds Solanum tuberosum ? - ? 94619 3.2.1.68 phytoglycogen + H2O - Solanum tuberosum ? - ? 374355 3.2.1.68 phytoglycogen + H2O little activity Solanum tuberosum ? - ? 374355 3.2.1.68 phytoglycogen + H2O short chains of DP3 and DP4 are most effectively liberated by isoform ISA1 during the short period, and with the elapse of the incubation time intermediate chains with the peak of DP6 are more significantly produced. Almost only short chains of DP3 and DP4 are found after the isoform ISA3 reaction for 5-10 min, then during the course of enzymatic reaction until 60 min a small amount of the DP5-10 chains is found Oryza sativa ? - ? 374355 3.2.1.68 phytoglycogen + H2O the enzyme can debranch almost all of component chains of phytoglycogen Pseudomonas amyloderamosa ? - ? 374355 3.2.1.68 phytoglycogen + H2O the enzyme removes only exclusively chains of DP3 and DP4 when incubated with phytoglycogen while DP5-7 chains are slightly liberated after the prolonged incubation Escherichia coli ? - ? 374355 3.2.1.68 phytoglycogen + H2O the liberated chains formed two distinct peaks: the first large peak of DP3-4 and the second small peak of DP5-10 Crocosphaera subtropica ATCC 51142 ? - ? 374355 3.2.1.68 phytoglycogen + H2O the liberated chains from phytoglycogen are enriched in short chains of DP3-6, but contained longer chains of DP higer than 7, decreasing in amounts with the increase in chain-length until DP about 15 Synechococcus elongatus ? - ? 374355 3.2.1.68 phytoglycogen + H2O the liberated chains from phytoglycogen are enriched in short chains of DP3-6, but contained longer chains of DP higer than 7, decreasing in amounts with the increase in chain-length until DP about 15 Synechococcus elongatus PCC7942 ? - ? 374355 3.2.1.68 phytoglycogen + H2O - Oryza sativa maltose + maltooligosaccharides - ? 393250 3.2.1.68 potato amylopectin + H2O - Arabidopsis thaliana ? - ? 432308 3.2.1.68 potato starch + H2O - Lederbergia lentus ? - ? 401777 3.2.1.68 potato starch, boiled + H2O best substrate Solanum tuberosum ? - ? 374400 3.2.1.68 potato starch, boiled + H2O little activity Solanum tuberosum ? - ? 374400 3.2.1.68 pullulan + H2O - Solanum tuberosum ? - ? 94190 3.2.1.68 pullulan + H2O little activity Solanum tuberosum ? - ? 94190 3.2.1.68 pullulan + H2O - Phaseolus vulgaris linear maltooligosaccharides - ? 393379 3.2.1.68 saccharose + H2O activity 67% Lipomyces kononenkoae ? - ? 94620 3.2.1.68 soluble starch + H2O - Lederbergia lentus ? - ? 94576 3.2.1.68 soluble starch + H2O - Bacillus sp. (in: Bacteria) ? - ? 94576 3.2.1.68 soluble starch + H2O - Bacillus sp. (in: Bacteria) CICIM 304 ? - ? 94576 3.2.1.68 starch + H2O - Zea mays ? - r 94621 3.2.1.68 starch + H2O - Solanum tuberosum ? - ? 94621 3.2.1.68 starch + H2O - Oryza sativa ? - ? 94621 3.2.1.68 starch + H2O - Lipomyces kononenkoae ? - ? 94621 3.2.1.68 starch + H2O - Pseudomonas amyloderamosa ? - ? 94621 3.2.1.68 starch + H2O soluble starch, immobilized on Sepharose 4B Solanum tuberosum ? - ? 94621 3.2.1.68 starch + H2O debranching of Cassava starch in concert with pullulanase yielding resistant starch type III. Effects of starch concentration, isoamylase concentration and incubation time on hydrolysis of cassava starch, overview Pseudomonas sp. ? - ? 94621 3.2.1.68 starch + H2O hydrolysis of alpha-1,6-glycosidic linkages Amaranthus cruentus ? - ? 94621 3.2.1.68 starch + H2O specific hydrolysis of alpha-1,6-glucosidic linkages Chlamydomonas reinhardtii ? detection of aminobenzamide-labeled maltooligosaccharide products, overview ? 94621 3.2.1.68 starch + H2O specific hydrolysis of alpha-1,6-glucosidic linkages Chlamydomonas reinhardtii 330 ? detection of aminobenzamide-labeled maltooligosaccharide products, overview ? 94621 3.2.1.68 starch + H2O - Arabidopsis thaliana linear maltooligosaccharides - ? 393541 3.2.1.68 starch + H2O - Oryza sativa maltose + maltooligosaccharides - ? 393542 3.2.1.68 starch + H2O the enzyme has the ability to attack side chains composed of 1-3 glucose residues Lederbergia lentus amylose - ? 446356 3.2.1.68 sticky rice starch + H2O - Bacillus sp. (in: Bacteria) ? - ? 432389 3.2.1.68 waxy corn amylopectin + H2O - Thermobifida fusca ? - ? 448250 3.2.1.68 waxy rice starch + H2O - Lederbergia lentus ? - ? 448253