3.2.1.57	4(2)-alpha-isomaltosylisomaltose + H2O	cleaves the alpha-1,4-glycosidic linkage of the panose motif	Aspergillus niger	?	-	?	371899	
3.2.1.57	4(3)-alpha-panosylpanose + H2O	cleaves the alpha-1,4-glycosidic linkage of the panose motif	Aspergillus niger	?	-	?	371900	
3.2.1.57	6(2)-alpha-isomaltosylmaltose + H2O	isomaltotriosylglucose, cleaves the alpha-1,4-glycosidic linkage of the panose motif	Aspergillus niger	?	-	?	372176	
3.2.1.57	6(2)-alpha-maltosylmaltose + H2O	cleaves the alpha-1,4-glycosidic linkage of the panose motif	Aspergillus niger	?	-	?	372177	
3.2.1.57	6(3)-alpha-glucosylmaltotriose + H2O	cleaves the alpha-1,4-glycosidic linkage of the panose motif	Aspergillus niger	?	-	?	372178	
3.2.1.57	alpha-glucosylmaltotriose + H2O	-	Aspergillus niger	isomaltose + maltose	-	?	94468	
3.2.1.57	alpha-maltosylmaltose + H2O	-	Aspergillus niger	isopanose + glucose	-	?	94469	
3.2.1.57	azidopullulan + H2O	-	Aspergillus niger	?	-	?	94471	
3.2.1.57	additional information	not: amylose, maltoheptaose, starch, amylopectin	Bacillus sp. (in: Bacteria)	?	-	?	89	
3.2.1.57	additional information	recombinant IPU hydrolyzes various substrates containing the structure of panose indicating a strict subsite recognition of the panose motif, cleaves the alpha-1,4-glycosidic linkage	Aspergillus niger	?	-	?	89	
3.2.1.57	additional information	not: amylose, maltoheptaose, starch, amylopectin	Bacillus sp. (in: Bacteria) US149	?	-	?	89	
3.2.1.57	panose + H2O	-	Aspergillus niger	?	-	?	94191	
3.2.1.57	panose + H2O	-	Aspergillus niger	isomaltose + glucose	-	?	94470	
3.2.1.57	panose + H2O	cleaves the alpha-1,4-glycosidic linkage	Aspergillus niger	isomaltose + glucose	-	?	94470	
3.2.1.57	pullulan + H2O	-	Aspergillus niger	isopanose + (Glc)4	-	?	94466	
3.2.1.57	pullulan + H2O	-	Aspergillus niger	isopanose + (Glc)4	small amount	?	94466	
3.2.1.57	pullulan + H2O	-	Arthrobacter globiformis	isopanose + (Glc)6	-	?	94467	
3.2.1.57	pullulan + H2O	-	Arthrobacter globiformis T6	isopanose + (Glc)6	-	?	94467	
3.2.1.57	pullulan + H2O	cleaves the alpha-1,4-glycosidic linkage	Aspergillus niger	isopanose	-	?	94771	
3.2.1.57	pullulan + H2O	IPU degrades alpha-1,4-glycosidic linkage from the reducing end adjacent to alpha-1,6-glycosidic linkage in pullulan	Bacillus sp. (in: Bacteria)	isopanose	-	?	94771	
3.2.1.57	pullulan + H2O	very strict substrate specificity, preferred over panose	Aspergillus niger	isopanose	-	?	94771	
3.2.1.57	pullulan + H2O	the enzyme cleaves the alpha(1,4) glucosidic linkage using an inverting mechanism and liberating D-glucose	Aspergillus brasiliensis	isopanose	trisaccharide [Glc-alpha(1,4)-Glc-alpha(1,6)-Glc]	?	94771	
3.2.1.57	pullulan + H2O	IPU degrades alpha-1,4-glycosidic linkage from the reducing end adjacent to alpha-1,6-glycosidic linkage in pullulan	Bacillus sp. (in: Bacteria) US149	isopanose	-	?	94771	
3.2.1.57	pullulan + H2O	the enzyme cleaves the alpha(1,4) glucosidic linkage using an inverting mechanism and liberating D-glucose	Aspergillus brasiliensis ATCC 9642	isopanose	trisaccharide [Glc-alpha(1,4)-Glc-alpha(1,6)-Glc]	?	94771	
3.2.1.57	pullulan + H2O	-	Aspergillus niger	isopanose + ?	-	?	382685	
3.2.1.57	pullulan + H2O	-	Aureobasidium melanogenum	isopanose + ?	-	?	382685	
3.2.1.57	pullulan + H2O	Asp353, Asp372 and Asp373 are the catalytic residues of IPU	Aspergillus niger	isopanose + ?	-	?	382685	
3.2.1.57	pullulan + H2O	-	Aureobasidium melanogenum P16	isopanose + ?	-	?	382685	
3.2.1.57	pullulan + H2O	Asp353, Asp372 and Asp373 are the catalytic residues of IPU	Aspergillus niger ATCC 9642	isopanose + ?	-	?	382685