3.2.1.4 2 cellobiose transfer reaction Trichoderma viride cellotetraose + H2O - ? 259818 3.2.1.4 2',4'-dinitrophenyl-beta-D-cellobiose + H2O - Cellulomonas fimi ? - ? 259824 3.2.1.4 2,3-di-O-methylated cellulase + H2O 2,3-di-O-methylated cellulase, having a trace amount of unsubstituted glucose units is hydrolyzed. Only the linkages between glucose units and 2,3-di-O-methylated units are cleaved. No hydrolysis of 2,3-di-O-methylcellulose, having every structural unit of them regioselectively substituted Trichoderma viride ? - ? 259820 3.2.1.4 2,4-dinitrophenyl beta-cellobioside + H2O - Cellulomonas fimi ? - ? 446621 3.2.1.4 2,4-dinitrophenyl beta-cellobioside + H2O - Cellulomonas fimi NBRC 15513 ? - ? 446621 3.2.1.4 2,4-dinitrophenyl beta-cellobioside + H2O - Cellulomonas fimi NCIMB 8980 ? - ? 446621 3.2.1.4 2,4-dinitrophenyl beta-cellobioside + H2O - Cellulomonas fimi NCTC 7547 ? - ? 446621 3.2.1.4 2,4-dinitrophenyl beta-cellobioside + H2O - Cellulomonas fimi ATCC 484 ? - ? 446621 3.2.1.4 2,4-dinitrophenyl beta-cellobioside + H2O - Cellulomonas fimi JCM 1341 ? - ? 446621 3.2.1.4 2,4-dinitrophenyl beta-cellobioside + H2O - Cellulomonas fimi DSM 20113 ? - ? 446621 3.2.1.4 2,4-dinitrophenyl beta-cellotrioside + H2O cleavage occurs between the second and third sugar residues with no release of chromophore, thus no colour change Cellulomonas fimi 2,4-dinitrophenyl beta-cellobioside + D-glucose - ? 446622 3.2.1.4 2,4-dinitrophenyl beta-D-cellobioside + H2O - Thermobifida fusca ? - ? 380895 3.2.1.4 2,4-dinitrophenyl beta-D-cellobioside + H2O - Thermobifida fusca Cel9A ? - ? 380895 3.2.1.4 2-hydroxyethyl cellulose + H2O - Geobacillus thermodenitrificans ? - ? 446631 3.2.1.4 2-hydroxyethyl cellulose + H2O - Geobacillus thermodenitrificans T12 ? - ? 446631 3.2.1.4 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside + H2O specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture Thermotoga maritima 4'',6''-O-benzylidene beta-cellobioside + 2-chloro-4-nitrophenyl beta-D-glucoside - ? 429740 3.2.1.4 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside + H2O specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture Trichoderma longibrachiatum 4'',6''-O-benzylidene beta-cellobioside + 2-chloro-4-nitrophenyl beta-D-glucoside - ? 429740 3.2.1.4 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside + H2O specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture Bacillus amyloliquefaciens 4'',6''-O-benzylidene beta-cellobioside + 2-chloro-4-nitrophenyl beta-D-glucoside - ? 429740 3.2.1.4 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside + H2O specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture Thermotoga maritima DSM 3109 4'',6''-O-benzylidene beta-cellobioside + 2-chloro-4-nitrophenyl beta-D-glucoside - ? 429740 3.2.1.4 4-methylumbelliferyl beta-cellotrioside + H2O - Thermobifida fusca ? - ? 399668 3.2.1.4 4-methylumbelliferyl beta-D-cellobioside + H2O - Saccharolobus solfataricus ? - ? 115827 3.2.1.4 4-methylumbelliferyl beta-D-lactoside + H2O - Rasamsonia emersonii 4-methylumbelliferone + D-lactose - ? 440875 3.2.1.4 4-methylumbelliferyl cellobioside + H2O - Niallia circulans ? - ? 259825 3.2.1.4 4-methylumbelliferyl cellobioside + H2O - Bacillus cellulyticus K-12 ? - ? 259825 3.2.1.4 4-methylumbelliferyl cellobioside + H2O - Trichoderma reesei 4-methylumbelliferone + cellobiose - ? 435143 3.2.1.4 4-methylumbelliferyl-beta-D-lactoside + H2O - Melanocarpus albomyces 4-methylumbelliferone + D-lactose - ? 372027 3.2.1.4 4-methylumbelliferyl-beta-D-lactoside + H2O very low activity Melanocarpus albomyces 4-methylumbelliferone + D-lactose - ? 372027 3.2.1.4 4-methylumbelliferyl-beta-D-lactoside + H2O - Melanocarpus albomyces ALKO4237 4-methylumbelliferone + D-lactose - ? 372027 3.2.1.4 4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside + H2O - Trichoderma longibrachiatum 4,6-O-(3-oxobutylidene)-beta-D-cellotriose + 4-nitrophenyl-beta-D-cellobioside - ? 442936 3.2.1.4 4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside + H2O - Bacillus amyloliquefaciens 4,6-O-(3-oxobutylidene)-beta-D-cellotriose + 4-nitrophenyl-beta-D-cellobioside - ? 442936 3.2.1.4 4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-cellopentaoside + H2O - Aspergillus niger 4,6-O-(3-oxobutylidene)-beta-D-cellotriose + 4-nitrophenyl-beta-D-cellobioside - ? 442936 3.2.1.4 4-nitrophenyl beta-D-cellobioside + H2O 0.16% of the activity with carboxymethyl cellulose Thermosipho sp. LD-2008 4-nitrophenol + cellobiose - ? 372072 3.2.1.4 4-nitrophenyl beta-D-cellobioside + H2O 7.7% of the activity with carboxymethyl cellulose Xanthomonas sp. EC102 ? - ? 381178 3.2.1.4 4-nitrophenyl beta-D-glucopyranoside + H2O low activity Thermothelomyces thermophilus 4-nitrophenol + D-glucopyranose - ? 94269 3.2.1.4 4-nitrophenyl beta-D-glucopyranoside + H2O low activity Thermothelomyces thermophilus D-14 4-nitrophenol + D-glucopyranose - ? 94269 3.2.1.4 4-nitrophenyl beta-D-glucopyranoside + H2O 33.2% of the activtiy with carboxymethyl cellulose bacterium enrichment culture 4-nitrophenol + beta-D-glucose - ? 140033 3.2.1.4 4-nitrophenyl beta-D-glucopyranoside + H2O - Gracilibacillus sp. 4-nitrophenol + D-glucose - ? 361143 3.2.1.4 4-nitrophenyl beta-D-glucopyranoside + H2O - Gracilibacillus sp. SK1 4-nitrophenol + D-glucose - ? 361143 3.2.1.4 4-nitrophenyl beta-D-glucoside + H2O 33.2% of the activity with carboxymethyl cellulose bacterium enrichment culture 4-nitrophenol + D-glucose - ? 13708 3.2.1.4 4-nitrophenyl beta-D-glucoside + H2O - Aspergillus niger 4-nitrophenol + beta-D-glucose 14% of the activity with carboxymethyl cellulose ? 364700 3.2.1.4 4-nitrophenyl beta-D-glucoside + H2O low activity Pyrococcus furiosus 4-nitrophenol + beta-D-glucose - ? 364700 3.2.1.4 4-nitrophenyl beta-D-glucoside + H2O - Aspergillus niger HO 4-nitrophenol + beta-D-glucose 14% of the activity with carboxymethyl cellulose ? 364700 3.2.1.4 4-nitrophenyl cellobioside + H2O - Bacillus subtilis 4-nitrophenol + cellobiose - ? 372063 3.2.1.4 4-nitrophenyl cellobioside + H2O - Pyrococcus horikoshii 4-nitrophenol + cellobiose - ? 372063 3.2.1.4 4-nitrophenyl cellobioside + H2O - Pyrococcus furiosus 4-nitrophenol + cellobiose - ? 372063 3.2.1.4 4-nitrophenyl cellobioside + H2O - Pyrococcus horikoshii DSM 12428 4-nitrophenol + cellobiose - ? 372063 3.2.1.4 4-nitrophenyl cellobioside + H2O - Bacillus subtilis JS2004 4-nitrophenol + cellobiose - ? 372063 3.2.1.4 4-nitrophenyl cellobioside + H2O - Desulfurococcaceae archaeon EBI-244 ? - ? 418309 3.2.1.4 4-nitrophenyl cellopentaoside + H2O - Pyrococcus furiosus 4-nitrophenol + cellopentaose - ? 422631 3.2.1.4 4-nitrophenyl cellotetraoside + H2O - Pyrococcus furiosus 4-nitrophenol + cellotetraose - ? 422632 3.2.1.4 4-nitrophenyl cellotrioside + H2O - Pyrococcus furiosus 4-nitrophenol + cellotriose - ? 422633 3.2.1.4 4-nitrophenyl D-cellobioside + H2O - uncultured bacterium 4-nitrophenol + D-cellobiose - ? 431393 3.2.1.4 4-nitrophenyl D-cellobioside + H2O - Trichoderma reesei 4-nitrophenol + cellobiose - ? 435154 3.2.1.4 6-chloro-4-methylumbelliferyl beta-cellobioside + H2O - Cellulomonas fimi 6-chloro-4-methylumbelliferone + cellobiose - ? 446884 3.2.1.4 6-O-methylcellulose + H2O 6-O-methylcellulose having every structural unit of them regioselectively substituted Trichoderma viride additional information glycosidic bond between two adjacent substituted units can be cleaved to give oligomers with a degree of polymerization of ca. 8 ? 259819 3.2.1.4 acid swollen cellulose + H2O endoglucanase with exohydrolytic activity Xylella fastidiosa cellobiose + cellotriose - ? 391176 3.2.1.4 acid-swollen cellulose + H2O - Acetivibrio thermocellus ? - ? 372252 3.2.1.4 acid-swollen cellulose + H2O - Melanocarpus albomyces ? - ? 372252 3.2.1.4 acid-swollen cellulose + H2O - [Eubacterium] cellulosolvens ? - ? 372252 3.2.1.4 acid-swollen cellulose + H2O - Clostridium cellulovorans ? - ? 372252 3.2.1.4 acid-swollen cellulose + H2O activity of truncated enzyme form Ruminococcus albus ? - ? 372252 3.2.1.4 acid-swollen cellulose + H2O cleaved cellulose randomly Clostridium cellulovorans ? - ? 372252 3.2.1.4 acid-swollen cellulose + H2O activity of truncated enzyme form Ruminococcus albus F-40 ? - ? 372252 3.2.1.4 acid-swollen cellulose + H2O - Acetivibrio thermocellus CelJ ? - ? 372252 3.2.1.4 acid-swollen cellulose + H2O - Melanocarpus albomyces ALKO4237 ? - ? 372252 3.2.1.4 alpha-cellulose + H2O activity of the catalytic module EG1-CM is 58% of the activity with endoglucanase 1 Volvariella volvacea ? - ? 372303 3.2.1.4 alpha-chitin + H2O due to the binding, it seems chitin would be an inhibitor of Cel6B, Cel9A and Cel48A, but not of Cel6A and Cel5A Thermobifida fusca ? - ? 391228 3.2.1.4 amorphic Solca Floc cellulose + H2O - Melanocarpus albomyces ? - ? 441042 3.2.1.4 amorphic Solca Floc cellulose + H2O - Melanocarpus albomyces ALKO4237 ? - ? 441042 3.2.1.4 amorphous cellulose + H2O - Bursaphelenchus xylophilus ? - ? 399951 3.2.1.4 arabinan + H2O - Saccharolobus solfataricus ? - ? 93968 3.2.1.4 arabinan + H2O hydrolysed at 53% compared to the hydrolysis of carboxymethylcellulose Saccharolobus solfataricus ? - ? 93968 3.2.1.4 avicel + H2O - Bacillus subtilis ? - ? 259817 3.2.1.4 avicel + H2O - Bacillus sp. (in: Bacteria) ? - ? 259817 3.2.1.4 avicel + H2O - Trichoderma viride ? - ? 259817 3.2.1.4 avicel + H2O - Trichoderma reesei ? - ? 259817 3.2.1.4 avicel + H2O - Thermotoga maritima ? - ? 259817 3.2.1.4 avicel + H2O - Pyrococcus horikoshii ? - ? 259817 3.2.1.4 avicel + H2O - Talaromyces funiculosus ? - ? 259817 3.2.1.4 avicel + H2O - Thermochaetoides thermophila ? - ? 259817 3.2.1.4 avicel + H2O - Mycothermus thermophilus ? - ? 259817 3.2.1.4 avicel + H2O - Ruminiclostridium josui ? - ? 259817 3.2.1.4 avicel + H2O - Cellulomonas sp. ? - ? 259817 3.2.1.4 avicel + H2O - Gloeophyllum trabeum ? - ? 259817 3.2.1.4 avicel + H2O - Clostridium cellulovorans ? - ? 259817 3.2.1.4 avicel + H2O - Melanocarpus albomyces ? - ? 259817 3.2.1.4 avicel + H2O - Brassica napus ? - ? 259817 3.2.1.4 avicel + H2O - Thermothelomyces thermophilus ? - ? 259817 3.2.1.4 avicel + H2O - Paenibacillus barcinonensis ? - ? 259817 3.2.1.4 avicel + H2O - Rasamsonia emersonii ? - ? 259817 3.2.1.4 avicel + H2O - Xanthomonas campestris pv. campestris ? - ? 259817 3.2.1.4 avicel + H2O - Trichoderma atroviride ? - ? 259817 3.2.1.4 avicel + H2O - Irpex lacteus ? glucose + cellobiose + cellotriose + cellotetraose ? 259817 3.2.1.4 avicel + H2O - Bacillus cellulyticus K-12 ? cellobiose + cellotetraose ? 259817 3.2.1.4 avicel + H2O - Niallia circulans ? main product is cellobiose ? 259817 3.2.1.4 avicel + H2O - Actinomyces sp. 40 ? 8.1% of the activity with carboxymethyl cellulose ? 259817 3.2.1.4 avicel + H2O weak activity Ruminiclostridium cellulolyticum ? - ? 259817 3.2.1.4 avicel + H2O weak activity Acetivibrio thermocellus ? - ? 259817 3.2.1.4 avicel + H2O low activity Aspergillus aculeatus ? - ? 259817 3.2.1.4 avicel + H2O no hydrolysis Acetivibrio thermocellus ? - ? 259817 3.2.1.4 avicel + H2O no hydrolysis Ruminiclostridium cellobioparum ? - ? 259817 3.2.1.4 avicel + H2O cellulase I Fusarium verticillioides ? - ? 259817 3.2.1.4 avicel + H2O chimeric xylanase/endoglucanase Cellulomonas fimi ? - ? 259817 3.2.1.4 avicel + H2O endoglucanase I show very low activity Acetivibrio thermocellus ? - ? 259817 3.2.1.4 avicel + H2O 14% of the activity with carboxymethylcellulose Bacillus amyloliquefaciens ? - ? 259817 3.2.1.4 avicel + H2O 33% of the activity with carboxymethyl cellulose Salipaludibacillus agaradhaerens ? - ? 259817 3.2.1.4 avicel + H2O activity of the catalytic module EG1-CM is43% of the activity with endoglucanase 1 Volvariella volvacea ? - ? 259817 3.2.1.4 avicel + H2O endoglucanase 47 Fomitopsis palustris ? - ? 259817 3.2.1.4 avicel + H2O microcrystalline cellulose (Avicel) is subjected to 3 different pretreatments (acid, alkaline, and organic solvent) before exposure to amixture of cellulases Trichoderma reesei ? - ? 259817 3.2.1.4 avicel + H2O microcrystalline cellulose, shows poor growth and enzyme production, relative activity 5.2% Bacillus sp. (in: Bacteria) ? - ? 259817 3.2.1.4 avicel + H2O 12% of the activity with carboxymethyl cellulose Ganoderma lucidum ? - ? 259817 3.2.1.4 avicel + H2O 21.8% of the activity with carboxymethyl cellulose bacterium enrichment culture ? - ? 259817 3.2.1.4 avicel + H2O 21.8% of the activtiy with carboxymethyl cellulose bacterium enrichment culture ? - ? 259817 3.2.1.4 avicel + H2O 55% of the activity with carboxymethyl cellulose Xanthomonas sp. EC102 ? - ? 259817 3.2.1.4 avicel + H2O 14% of the activity with carboxymethyl-cellulose Phialophora sp. CGMCC 3328 ? - ? 259817 3.2.1.4 avicel + H2O 17.7% of the activity with carboxymethyl-cellulose Phialophora sp. CGMCC 3328 ? - ? 259817 3.2.1.4 avicel + H2O good substrate of isoforms cellulase II and III Thermoascus aurantiacus ? - ? 259817 3.2.1.4 avicel + H2O fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus Teleogryllus emma ? - ? 259817 3.2.1.4 avicel + H2O - Pyrococcus horikoshii DSM 12428 ? - ? 259817 3.2.1.4 avicel + H2O - Bacillus sp. (in: Bacteria) HR68 ? - ? 259817 3.2.1.4 avicel + H2O 33% of the activity with carboxymethyl cellulose Salipaludibacillus agaradhaerens JAM-KU023 ? - ? 259817 3.2.1.4 avicel + H2O weak activity Acetivibrio thermocellus CelJ ? - ? 259817 3.2.1.4 avicel + H2O microcrystalline cellulose, shows poor growth and enzyme production, relative activity 5.2% Bacillus sp. (in: Bacteria) CTP-09 ? - ? 259817 3.2.1.4 avicel + H2O - Thermochaetoides thermophila CT2 ? - ? 259817 3.2.1.4 avicel + H2O - Bacillus sp. (in: Bacteria) CH43 ? - ? 259817 3.2.1.4 avicel + H2O - Paenibacillus barcinonensis MG7 ? - ? 259817 3.2.1.4 avicel + H2O - Bacillus subtilis JS2004 ? - ? 259817 3.2.1.4 avicel + H2O - Trichoderma reesei QM6a ? - ? 259817 3.2.1.4 avicel + H2O - Pyrococcus horikoshii OT-3 ? - ? 259817 3.2.1.4 avicel + H2O - Actinomyces sp. 40 Korean Native Goat 40 ? 8.1% of the activity with carboxymethyl cellulose ? 259817 3.2.1.4 avicel + H2O - Xanthomonas campestris pv. campestris DSM 3586 ? - ? 259817 3.2.1.4 avicel + H2O 14% of the activity with carboxymethylcellulose Bacillus amyloliquefaciens DL-3 ? - ? 259817 3.2.1.4 avicel + H2O - Pyrococcus horikoshii cellobiose + ? - ? 391390 3.2.1.4 avicel + H2O the main product is cellobiose Pyrococcus horikoshii cellobiose + ? - ? 391390 3.2.1.4 avicel + H2O the main product is cellobiose Pyrococcus horikoshii DSM 12428 cellobiose + ? - ? 391390 3.2.1.4 avicel + H2O - Pyrococcus horikoshii OT-3 cellobiose + ? - ? 391390 3.2.1.4 avicel + H2O cellooligosaccharides production from Avicel is detected only when 0.00185 mkat of Xf818his are employed, and not when 16.67 nkat are used. Endoglucanase with exohydrolytic activity Xylella fastidiosa cellobiose + cellotriose - ? 391391 3.2.1.4 avicel + H2O substrate concentration 1%, low activity uncultured bacterium cellobiose + cellotriose - ? 391391 3.2.1.4 avicel + H2O the main product is cellobiose together with a small amount of glucose, endoglucanase 35 Fomitopsis palustris cellobiose + glucose - ? 391392 3.2.1.4 avicel + H2O - Humicola insolens D-glucose + ? - ? 435345 3.2.1.4 avicel + H2O - Fusarium oxysporum D-glucose + ? - ? 435345 3.2.1.4 avicel + H2O - Humicola insolens YH-8 D-glucose + ? - ? 435345 3.2.1.4 Avicel PH-101 + H2O low activity uncultured bacterium ? - ? 431640 3.2.1.4 azurine-labelled hydroxyethylcellulose + H2O - Bacillus subtilis ? - ? 443229 3.2.1.4 azurine-labelled hydroxyethylcellulose + H2O - Castellaniella defragrans ? - ? 443229 3.2.1.4 azurine-labelled hydroxyethylcellulose + H2O - Halosimplex carlsbadense ? - ? 443229 3.2.1.4 bacterial cellulose + H2O - Bacillus licheniformis ? - ? 372489 3.2.1.4 bacterial cellulose + H2O - Bacillus licheniformis NCIMB 9375 ? - ? 372489 3.2.1.4 bacterial cellulose + H2O - Bacillus licheniformis Gibson 46 ? - ? 372489 3.2.1.4 bacterial cellulose + H2O - Bacillus licheniformis JCM 2505 ? - ? 372489 3.2.1.4 bacterial cellulose + H2O - Bacillus licheniformis NRRL NRS-1264 ? - ? 372489 3.2.1.4 bacterial cellulose + H2O - Bacillus licheniformis NBRC 12200 ? - ? 372489 3.2.1.4 bacterial cellulose + H2O - Bacillus licheniformis ATCC 14580 ? - ? 372489 3.2.1.4 bacterial cellulose + H2O - Bacillus licheniformis DSM 13 ? - ? 372489 3.2.1.4 bacterial microcrystalline cellulose + H2O - Thermobifida fusca ? - ? 372490 3.2.1.4 bacterial microcrystalline cellulose + H2O weak activity Ruminiclostridium cellulolyticum ? - ? 372490 3.2.1.4 bacterial microcrystalline cellulose + H2O - Thermobifida fusca Cel9A ? - ? 372490 3.2.1.4 bacterial microcrystalline cellulose + H2O weak activity Ruminiclostridium cellulolyticum cellotetraose 83% cellotetraose + 11% cellotriose + 4% cellobiose ? 372491 3.2.1.4 bacterial micropcrystalline cellulose + H2O - Thermobifida fusca ? - ? 372492 3.2.1.4 bagasse + H2O steam-exploded bagasse Bacillus subtilis ? - ? 429968 3.2.1.4 ball-milled cellulose + H2O - Trichoderma reesei ? - ? 391399 3.2.1.4 barley 1,3-1,4-beta glucan + H2O - Acetivibrio thermocellus ? - ? 443231 3.2.1.4 barley beta-1,4-D-glucan + H2O best endoglucanase substrate Thermochaetoides thermophila ? - ? 447152 3.2.1.4 barley beta-D-glucan + H2O - Saccharolobus solfataricus ? - ? 391400 3.2.1.4 barley beta-glucan + H2O - Mycothermus thermophilus ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Acetivibrio thermocellus ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Talaromyces verruculosus ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Thermotoga petrophila ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Rasamsonia emersonii ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - uncultured bacterium ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Phanerodontia chrysosporium ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Humicola insolens ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Aspergillus fischeri ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Paenibacillus sp. M33 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Cryptopygus antarcticus ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Aspergillus terreus ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Paenibacillus sp. Y2 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Geobacillus thermodenitrificans ? - ? 372495 3.2.1.4 barley beta-glucan + H2O best substrate Saccharolobus shibatae ? - ? 372495 3.2.1.4 barley beta-glucan + H2O soluble substrate Bacillus subtilis ? - ? 372495 3.2.1.4 barley beta-glucan + H2O moderate activity uncultured bacterium ? - ? 372495 3.2.1.4 barley beta-glucan + H2O the highest specific activity toward polysaccharides occurs with mixed-linkage (1->3),(1->4)-beta-D-glucans such as barley beta-glucan and lichenan Pyrococcus furiosus ? - ? 372495 3.2.1.4 barley beta-glucan + H2O 136% of the activity with carboxymethyl cellulose Saccharolobus solfataricus ? - ? 372495 3.2.1.4 barley beta-glucan + H2O 92% of the activity on carboxymethyl cellulose Alicyclobacillus vulcanalis ? - ? 372495 3.2.1.4 barley beta-glucan + H2O 311% of the activity with carboxymethylcellulose Paenibacillus sp. X4 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O as AZCL beta-glucan Cellulomonas fimi ? - ? 372495 3.2.1.4 barley beta-glucan + H2O as AZCL beta-glucan Cellulomonas fimi NBRC 15513 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Thermotoga petrophila DSM 13995 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Aspergillus terreus NIH 2624 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O as AZCL beta-glucan Cellulomonas fimi NCIMB 8980 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Aspergillus fischeri P1 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O as AZCL beta-glucan Cellulomonas fimi NCTC 7547 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Humicola insolens Y1 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O best substrate Saccharolobus shibatae B12 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O best substrate Saccharolobus shibatae DSM 5389 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O as AZCL beta-glucan Cellulomonas fimi ATCC 484 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O - Geobacillus thermodenitrificans T12 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O as AZCL beta-glucan Cellulomonas fimi JCM 1341 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O soluble substrate Bacillus subtilis JA18 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O as AZCL beta-glucan Cellulomonas fimi DSM 20113 ? - ? 372495 3.2.1.4 barley beta-glucan + H2O high activity Fervidobacterium nodosum D-glucose + ? - ? 437191 3.2.1.4 barley beta-glucan + H2O high activity Fervidobacterium nodosum ATCC 35602 D-glucose + ? - ? 437191 3.2.1.4 barley beta-glucan + H2O high activity Fervidobacterium nodosum DSM 5306 D-glucose + ? - ? 437191 3.2.1.4 barley beta-glucan + H2O best substrate, high activity, product analysis uncultured archaeon cellobiose + cellotriose + D-glucose - ? 447155 3.2.1.4 barley glucan + H2O - Bacillus subtilis ? - ? 372496 3.2.1.4 barley glucan + H2O the enzyme hydrolyzes mixed linked beta-(1-3)(1-4) substrates such as barley glucan and lichenan more strongly than carboxymethylcellulose Thermotoga maritima ? - ? 372496 3.2.1.4 barley glucan + H2O 120% of the activity with carboxymethyl-cellulose Phialophora sp. CGMCC 3328 ? - ? 372496 3.2.1.4 barley glucan + H2O 143% of the activity with carboxymethyl-cellulose Phialophora sp. CGMCC 3328 ? - ? 372496 3.2.1.4 barley glucan + H2O fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus Teleogryllus emma ? - ? 372496 3.2.1.4 barley glucan + H2O - Bacillus subtilis BME-15 ? - ? 372496 3.2.1.4 beechwood xylan + H2O hydrolysed at 84% compared to the hydrolysis of carboxymethylcellulose Saccharolobus solfataricus ? - ? 391405 3.2.1.4 beechwood xylan + H2O 10% of the activity with carboxymethyl cellulose Xanthomonas sp. EC102 ? - ? 391405 3.2.1.4 beechwood xylan + H2O fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus Teleogryllus emma ? - ? 391405 3.2.1.4 beta-1,4-D-glucan + H2O - Thermochaetoides thermophila ? - ? 443238 3.2.1.4 beta-1,4-D-glucan + H2O bifunctional endoglucanase/xylanase enzyme Thermochaetoides thermophila ? - ? 443238 3.2.1.4 beta-1,4-D-xylan + H2O bifunctional endoglucanase/xylanase enzyme Thermochaetoides thermophila ? - ? 447189 3.2.1.4 beta-D-glucan + H2O - Thermochaetoides thermophila ? - ? 404515 3.2.1.4 beta-glucan + H2O - Bacillus sp. (in: Bacteria) ? - ? 94321 3.2.1.4 beta-glucan + H2O - Phanerodontia chrysosporium ? - ? 94321 3.2.1.4 beta-glucan + H2O - Acetivibrio thermocellus ? - ? 94321 3.2.1.4 beta-glucan + H2O - Bacillus mycoides ? - ? 94321 3.2.1.4 beta-glucan + H2O - Orpinomyces joyonii ? - ? 94321 3.2.1.4 beta-glucan + H2O - Gloeophyllum trabeum ? - ? 94321 3.2.1.4 beta-glucan + H2O - Clostridium cellulovorans ? - ? 94321 3.2.1.4 beta-glucan + H2O - Thermotoga maritima ? - ? 94321 3.2.1.4 beta-glucan + H2O - Thermothelomyces thermophilus ? - ? 94321 3.2.1.4 beta-glucan + H2O - Paenibacillus barcinonensis ? - ? 94321 3.2.1.4 beta-glucan + H2O - Neurospora crassa ? - ? 94321 3.2.1.4 beta-glucan + H2O - Talaromyces verruculosus ? - ? 94321 3.2.1.4 beta-glucan + H2O - Fomitopsis palustris ? - ? 94321 3.2.1.4 beta-glucan + H2O endoglucanase EG25 Chrysosporium lucknowense ? - ? 94321 3.2.1.4 beta-glucan + H2O endoglucanase EG28 Chrysosporium lucknowense ? - ? 94321 3.2.1.4 beta-glucan + H2O endoglucanase EG44 Chrysosporium lucknowense ? - ? 94321 3.2.1.4 beta-glucan + H2O endoglucanase EG47 Chrysosporium lucknowense ? - ? 94321 3.2.1.4 beta-glucan + H2O endoglucanase EG51 Chrysosporium lucknowense ? - ? 94321 3.2.1.4 beta-glucan + H2O endoglucanase EG60 Chrysosporium lucknowense ? - ? 94321 3.2.1.4 beta-glucan + H2O 24.6% of the activity with carboxymethylcellulose Bacillus amyloliquefaciens ? - ? 94321 3.2.1.4 beta-glucan + H2O from Hordeum vulgare Bispora sp. MEY-1 ? - ? 94321 3.2.1.4 beta-glucan + H2O and lichenan, preferred substrates Bacillus subtilis ? - ? 94321 3.2.1.4 beta-glucan + H2O 400% of the activity with carboxymethyl cellulose Thermosipho sp. LD-2008 ? - ? 94321 3.2.1.4 beta-glucan + H2O degree of hydrolysis reaches 10%, products are oligosaccharides with degree of polymerization 2-10 Talaromyces verruculosus ? - ? 94321 3.2.1.4 beta-glucan + H2O - Talaromyces verruculosus DSM 2263 ? - ? 94321 3.2.1.4 beta-glucan + H2O and lichenan, preferred substrates Bacillus subtilis AS3 ? - ? 94321 3.2.1.4 beta-glucan + H2O - Bacillus sp. (in: Bacteria) HR68 ? - ? 94321 3.2.1.4 beta-glucan + H2O - Talaromyces verruculosus NRRL 1050 ? - ? 94321 3.2.1.4 beta-glucan + H2O - Talaromyces verruculosus CBS 388.48 ? - ? 94321 3.2.1.4 beta-glucan + H2O - Talaromyces verruculosus ATCC 10513 ? - ? 94321 3.2.1.4 beta-glucan + H2O - Bacillus sp. (in: Bacteria) CH43 ? - ? 94321 3.2.1.4 beta-glucan + H2O - Paenibacillus barcinonensis MG7 ? - ? 94321 3.2.1.4 beta-glucan + H2O - Neurospora crassa DSM 1257 ? - ? 94321 3.2.1.4 beta-glucan + H2O - Bacillus mycoides S122C ? - ? 94321 3.2.1.4 beta-glucan + H2O - Fomitopsis palustris FFPRI 0507 ? - ? 94321 3.2.1.4 beta-glucan + H2O - Talaromyces verruculosus IMI 040039 ? - ? 94321 3.2.1.4 beta-glucan + H2O 24.6% of the activity with carboxymethylcellulose Bacillus amyloliquefaciens DL-3 ? - ? 94321 3.2.1.4 beta-glucan + H2O - Phanerodontia chrysosporium cellotriose + ? - ? 441132 3.2.1.4 birchwood xylan + H2O - Rasamsonia emersonii ? - ? 391513 3.2.1.4 birchwood xylan + H2O - Thermoascus aurantiacus ? 88% saccharification within 36 h, preparation containing cellulase and xylanase ? 391513 3.2.1.4 birchwood xylan + H2O hydrolysed at 73% compared to the hydrolysis of carboxymethylcellulose Saccharolobus solfataricus ? - ? 391513 3.2.1.4 birchwood xylan + H2O 2% of the activity with carboxymethyl cellulose Ganoderma lucidum ? - ? 391513 3.2.1.4 birchwood xylan + H2O 44% of the activity with carboxymethylcellulose uncultured microorganism ? - ? 391513 3.2.1.4 birchwood xylan + H2O fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus Teleogryllus emma ? - ? 391513 3.2.1.4 birchwood xylan + H2O - Thermoascus aurantiacus RCKK ? 88% saccharification within 36 h, preparation containing cellulase and xylanase ? 391513 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus subtilis ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Rhizopus arrhizus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus sp. (in: Bacteria) ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Coprinopsis cinerea ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus amyloliquefaciens ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus pumilus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Humicola insolens ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Komagataeibacter xylinus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Trichoderma viride ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Streptomyces ruber ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Trichoderma reesei ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermobifida fusca ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermoascus aurantiacus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Phanerodontia chrysosporium ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermotoga maritima ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Aspergillus fumigatus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Ganoderma lucidum ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Pyrococcus horikoshii ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Aspergillus tubingensis ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - uncultured bacterium ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermochaetoides thermophila ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Aspergillus aculeatus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Mycothermus thermophilus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Trichoderma longibrachiatum ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Cellulomonas fimi ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Talaromyces pinophilus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Artemia salina ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Reticulitermes speratus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermothielavioides terrestris ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Volvariella volvacea ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Geobacillus sp. ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Ampullaria crossean ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Salipaludibacillus agaradhaerens ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - [Humicola] grisea var. thermoidea ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Gymnascella citrina ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Beltraniella portoricensis ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - [Eubacterium] cellulosolvens ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Lyrodus pedicellatus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Trametes hirsuta ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Acidothermus cellulolyticus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - uncultured microorganism ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bursaphelenchus xylophilus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Caecomyces sp. CR4 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Saccharolobus solfataricus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Coptotermes formosanus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Rhizobium leguminosarum bv. trifolii ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Cryptococcus sp. S-2 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Staphylotrichum coccosporum ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermotoga petrophila ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Gracilibacillus sp. ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - uncultured rumen bacterium ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Desulfurococcaceae archaeon EBI-244 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Pyrococcus furiosus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermothelomyces thermophilus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Batocera horsfieldi ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Paenibacillus barcinonensis ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Rasamsonia emersonii ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - bacterium enrichment culture ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Clostridium cellulovorans ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Neurospora crassa ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Xanthomonas sp. EC102 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermosipho sp. LD-2008 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Actinomyces sp. 40 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus vallismortis ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Cordyceps sp. ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Phialophora sp. CGMCC 3328 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Talaromyces verruculosus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Saccharolobus shibatae ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus licheniformis ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Aspergillus glaucus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Geobacillus thermodenitrificans ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Pseudoalteromonas sp. DY3 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermobia domestica ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Ctenolepisma longicaudatum ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus sp. SR22 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Mucor circinelloides ? no glucose, cellobiose and short chain celloolifgosaccharides are formed ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermoascus aurantiacus ? 22% saccharification within 36 h, preparation containing cellulase and xylanase ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Alicyclobacillus vulcanalis ? production of a mixture of cellobiose, cellotriose, cellotetraose and cellopentaose ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - bacterium enrichment culture ? products are mainly cello-oligosaccharides along with small amounts of glucose and cellobiose ? 94663 3.2.1.4 carboxymethyl cellulose + H2O best substrate Rhodococcus sp. ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O best substrate Gloeophyllum trabeum ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O best substrate Thermothelomyces thermophilus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O high activity Fomitopsis palustris ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O best substrate tested Thermochaetoides thermophila ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O 68% of the activity with barley beta-glucan Orpinomyces joyonii ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O activity of truncated enzyme form Ruminococcus albus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O endo-beta-1,4-glucanase EG27 shows high preference for Ampullaria crossean ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O endo-beta-1,4-glucanase EG45 shows high preference for Ampullaria crossean ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O endoglucanase EG25 Chrysosporium lucknowense ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O endoglucanase EG28 Chrysosporium lucknowense ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O endoglucanase EG44 Chrysosporium lucknowense ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O endoglucanase EG47 Chrysosporium lucknowense ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O endoglucanase EG51 Chrysosporium lucknowense ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O endoglucanase EG60 Chrysosporium lucknowense ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O Cel9A is a nonprocessive enzyme with endo-cellulase activities Cytophaga hutchinsonii ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O endoglucanase 35 Fomitopsis palustris ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O endoglucanase 47 Fomitopsis palustris ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O endoglucanase with exohydrolytic activity Xylella fastidiosa ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O processive endoglucanase activity Cellulomonas flavigena ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O moderate activity uncultured bacterium ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O release of reducing sugar, degradation Sclerotinia sclerotiorum ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O 13.6% of the activity with barley beta-glucan Humicola insolens ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O 18.6% of the activity with barley beta-glucan Aspergillus fischeri ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O best substrate of isoform cellulase III, no substrate of cellulase II Thermoascus aurantiacus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O medium and low viscosity Mycothermus thermophilus ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O 1% w/v CMC-Na Thermochaetoides thermophila ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O 2% solution Amphobotrys ricini ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O AZO-carboxymethyl cellulose Streptomyces sp. G12 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O bifunctional endoglucanase/xylanase enzyme Thermochaetoides thermophila ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus Teleogryllus emma ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O soluble, lower activity uncultured archaeon ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O activity of truncated enzyme form Ruminococcus albus F-40 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O 2% solution Amphobotrys ricini URM 5627 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermotoga petrophila DSM 13995 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus vallismortis RG-07 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermochaetoides thermophila IMI 039719 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Saccharolobus solfataricus P2 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O 18.6% of the activity with barley beta-glucan Aspergillus fischeri P1 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Mucor circinelloides NRRL 26519 ? no glucose, cellobiose and short chain celloolifgosaccharides are formed ? 94663 3.2.1.4 carboxymethyl cellulose + H2O processive endoglucanase activity Cellulomonas flavigena 531 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Clostridium cellulovorans DSM 3052 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermochaetoides thermophila DSM 1495 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Pyrococcus horikoshii DSM 12428 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus subtilis KG10 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus licheniformis NCIMB 9375 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Mycothermus thermophilus MTCC 4520 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Rhizobium leguminosarum bv. trifolii ANU843 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus licheniformis Gibson 46 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Talaromyces verruculosus DSM 2263 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermobifida fusca Cel9A ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Aspergillus tubingensis NKBP-55 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus licheniformis JCM 2505 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Aspergillus fumigatus M.7.1 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus subtilis AS3 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Talaromyces verruculosus NRRL 1050 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O 13.6% of the activity with barley beta-glucan Humicola insolens Y1 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermothelomyces thermophilus JCP 1-4 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O release of reducing sugar, degradation Sclerotinia sclerotiorum 1980 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Salipaludibacillus agaradhaerens JAM-KU023 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Talaromyces verruculosus CBS 388.48 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Saccharolobus shibatae B12 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus licheniformis NRRL NRS-1264 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermotoga maritima DSM 3109 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Gracilibacillus sp. SK1 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Saccharolobus shibatae DSM 5389 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - uncultured bacterium consortium XM70 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermoascus aurantiacus RBB-1 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus sp. (in: Bacteria) BG-CS10 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O best substrate tested Thermochaetoides thermophila CT2 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Talaromyces verruculosus ATCC 10513 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Rhizopus arrhizus CCT 7560 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Geobacillus thermodenitrificans T12 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Talaromyces pinophilus CF-2612 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Paenibacillus barcinonensis MG7 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Neurospora crassa DSM 1257 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermochaetoides thermophila CBS 144.50 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O high activity Fomitopsis palustris FFPRI 0507 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus subtilis JS2004 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus subtilis LFS3 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Aspergillus glaucus CCHA ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus sp. (in: Bacteria) DUSELR13 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Trichoderma reesei QM 9414 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Talaromyces verruculosus IMI 040039 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus licheniformis NBRC 12200 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Staphylotrichum coccosporum NBRC 31817 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus pumilus S124A ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Geobacillus sp. WSUCFI ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Acidothermus cellulolyticus E1 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermothelomyces thermophilus M.7.7 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermoascus aurantiacus RCKK ? 22% saccharification within 36 h, preparation containing cellulase and xylanase ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus licheniformis ATCC 14580 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Pyrococcus horikoshii OT-3 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Actinomyces sp. 40 Korean Native Goat 40 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Thermobifida fusca Cel6A ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus amyloliquefaciens DL-3 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus licheniformis DSM 13 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Bacillus sp. (in: Bacteria) HSH-810 ? - ? 94663 3.2.1.4 carboxymethyl cellulose + H2O - Lysobacter sp. cellobiose + ? - ? 391571 3.2.1.4 carboxymethyl cellulose + H2O - Lysobacter sp. IB-9374 cellobiose + ? - ? 391571 3.2.1.4 carboxymethyl cellulose + H2O - Humicola insolens D-glucose + ? - ? 400612 3.2.1.4 carboxymethyl cellulose + H2O - Fusarium oxysporum D-glucose + ? - ? 400612 3.2.1.4 carboxymethyl cellulose + H2O - Phanerodontia chrysosporium D-glucose + ? - ? 400612 3.2.1.4 carboxymethyl cellulose + H2O - Cellulomonas biazotea D-glucose + ? - ? 400612 3.2.1.4 carboxymethyl cellulose + H2O - Gymnascella citrina D-glucose + ? - ? 400612 3.2.1.4 carboxymethyl cellulose + H2O - Teleogryllus emma D-glucose + ? - ? 400612 3.2.1.4 carboxymethyl cellulose + H2O - Postia placenta D-glucose + ? - ? 400612 3.2.1.4 carboxymethyl cellulose + H2O - uncultured bacterium D-glucose + ? - ? 400612 3.2.1.4 carboxymethyl cellulose + H2O high activity Fervidobacterium nodosum D-glucose + ? - ? 400612 3.2.1.4 carboxymethyl cellulose + H2O high activity Fervidobacterium nodosum ATCC 35602 D-glucose + ? - ? 400612 3.2.1.4 carboxymethyl cellulose + H2O - Cellulomonas biazotea NIAB 442 D-glucose + ? - ? 400612 3.2.1.4 carboxymethyl cellulose + H2O - Humicola insolens YH-8 D-glucose + ? - ? 400612 3.2.1.4 carboxymethyl cellulose + H2O high activity Fervidobacterium nodosum DSM 5306 D-glucose + ? - ? 400612 3.2.1.4 carboxymethyl cellulose + H2O - Cherax destructor D-glucose + cellobiose + cellooligosaccharide - ? 400613 3.2.1.4 carboxymethyl cellulose + H2O - Cherax destructor Clark 1936 D-glucose + cellobiose + cellooligosaccharide - ? 400613 3.2.1.4 carboxymethyl cellulose + H2O - Gecarcoidea natalis glucose + cellobiose + short oligomers - ? 400614 3.2.1.4 carboxymethyl cellulose + H2O - Gecarcoidea natalis Pocock 1888 glucose + cellobiose + short oligomers - ? 400614 3.2.1.4 carboxymethyl cellulose + H2O - Cherax destructor additional information short glucose oligomers, cellobiose and a very small amount of glucose ? 404604 3.2.1.4 carboxymethyl cellulose + H2O - Cherax destructor Clark 1936 additional information short glucose oligomers, cellobiose and a very small amount of glucose ? 404604 3.2.1.4 carboxymethyl cellulose + H2O enzyme hydrolyzes internal beta-1,4 glycosidic bonds within lichenan as well as carboxymethyl cellulose to release oligosaccharides and glucose Euphausia pacifica cellotriose + cellobiose + D-glucose major products ? 430015 3.2.1.4 carboxymethyl cellulose + H2O - Saccharolobus solfataricus cellobiose + cellotriose + ? - ? 435380 3.2.1.4 carboxymethyl cellulose + H2O - Aspergillus niger cellobiose + cellotriose + ? products are 18% cellotriose, 72% cellobiose and 10% glucose ? 435380 3.2.1.4 carboxymethyl cellulose + H2O - Saccharolobus solfataricus DSM 1617 cellobiose + cellotriose + ? - ? 435380 3.2.1.4 carboxymethyl cellulose + H2O - Acetivibrio thermocellus cellobiose + cellooligosaccharides - ? 447263 3.2.1.4 carboxymethyl cellulose + H2O substrate concentration 1%, low activity uncultured bacterium cellobiose + cellotriose - ? 447264 3.2.1.4 carboxymethyl-cellulose + H2O - Saccharolobus solfataricus ? - ? 391574 3.2.1.4 carboxymethyl-cellulose + H2O - Bacillus subtilis ? - ? 391574 3.2.1.4 carboxymethyl-cellulose + H2O - Bacillus subtilis I15 ? - ? 391574 3.2.1.4 carboxymethylcellulose + H2O - Epicoccum sp. D-glucose + ? - ? 94531 3.2.1.4 carboxymethylcellulose + H2O - Dothideomycetes sp. D-glucose + ? - ? 94531 3.2.1.4 carboxymethylcellulose + H2O - Bacillus subtilis ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Neurospora crassa ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Aspergillus niger ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Rhizopus arrhizus ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Bacillus sp. (in: Bacteria) ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Bacillus amyloliquefaciens ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Niallia circulans ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Aspergillus oryzae ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Sclerotinia sclerotiorum ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Aspergillus sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Penicillium sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Exophiala sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Trichoderma reesei ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Thermobifida fusca ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Thermoascus aurantiacus ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Cladosporium sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Alternaria sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Thermothelomyces heterothallicus ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Thermochaetoides thermophila ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Aureobasidium sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Ruminiclostridium cellulolyticum ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Acetivibrio thermocellus ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Chloridium sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Trichoderma sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Phoma sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Stachybotrys chartarum ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Talaromyces verruculosus ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Brevibacillus sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Aquifex aeolicus ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Rasamsonia emersonii ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Volvariella volvacea ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Cellulomonas biazotea ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Psacothea hilaris ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Clostridium cellulovorans ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Melanocarpus albomyces ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Bispora sp. MEY-1 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Syncephalastrum racemosum ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Thermothelomyces thermophilus ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Caldicellulosiruptor saccharolyticus ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Alicyclobacillus acidocaldarius ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Bipolaris sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Microdochium sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Nigrospora sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Arthrinium sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Cephalosporium sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Sporothrix sp. ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Minimidochium sp. TMS-2011 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Escherichia coli ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Xanthomonas campestris pv. campestris ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Penicillium crustosum ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Dictyoglomus turgidum ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Paenibacillus sp. M33 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Bellamya chinensis laeta ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Ampullaria crossean ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Phanerodontia chrysosporium ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - uncultured microorganism ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Trichoderma asperellum ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Paenibacillus sp. X4 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Citrobacter farmeri ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Nilaparvata lugens ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Lysobacter enzymogenes ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Photobacterium panuliri ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Trichoderma atroviride ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Aspergillus terreus ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Paenibacillus sp. Y2 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O best substrate Bacillus mycoides ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O activity with carbomethylcellulose with approxomately 4 carboxymethyl groups per 10 anhydro-glucose units is about 3.5 times better than that with carbomethylcellulose with approximately 8 carboxymethyl groups Brassica napus ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O the enzyme hydrolyzes mixed linked beta-(1-3)(1-4) substrates such as barley glucan and lichenan more strongly than carboxymethylcellulose Thermotoga maritima ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O the smallest product is a disaccharide Acetivibrio thermocellus ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O soluble substrate Bacillus subtilis ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O high specific activity towards carboxymethylcellulose Fervidobacterium nodosum Rt17-B1 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O medium-viscosity substrate Thermobifida fusca ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Aspergillus terreus NIH 2624 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Niallia circulans KSM-N257 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Brevibacillus sp. JXL ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Lysobacter enzymogenes 521 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Bellamya chinensis laeta UM-2014 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Trichoderma asperellum PQ34 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Thermothelomyces thermophilus ATCC 424674 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Photobacterium panuliri DSM 27646 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Acetivibrio thermocellus 132 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Bacillus subtilis AS3 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Bacillus sp. (in: Bacteria) HR68 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Thermothelomyces heterothallicus CBS 202.75 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Aspergillus oryzae cmc-1 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Acetivibrio thermocellus CelJ ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Bacillus sp. (in: Bacteria) CTP-09 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Thermochaetoides thermophila CT2 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Cellulomonas biazotea NIAB 442 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Bacillus sp. (in: Bacteria) CH43 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Thermothelomyces heterothallicus CBS 663.74 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O best substrate Bacillus mycoides S122C ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Bacillus subtilis BME-15 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Thermothelomyces thermophilus D-14 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O soluble substrate Bacillus subtilis JA18 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Penicillium crustosum 601 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Xanthomonas campestris pv. campestris DSM 3586 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Bacillus amyloliquefaciens DL-3 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Syncephalastrum racemosum BCC18080 ? - ? 115813 3.2.1.4 carboxymethylcellulose + H2O - Phaseolus vulgaris additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Bacillus subtilis additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Aspergillus niger additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Populus x canadensis additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Pseudomonas fluorescens additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Neocallimastix frontalis additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Bacillus sp. (in: Bacteria) additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Niallia circulans additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Ruminococcus albus additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Dickeya chrysanthemi additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Aspergillus terreus additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Fibrobacter succinogenes additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Trichoderma viride additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Aspergillus candidus additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Catharanthus roseus additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Trichoderma reesei additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Caldicellulosiruptor saccharolyticus additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Thermobifida fusca additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Streptomyces lividans additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Fusarium verticillioides additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Thermoascus aurantiacus additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Aspergillus fumigatus additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Dolabella auricularia additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Thermothelomyces heterothallicus additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Talaromyces funiculosus additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Thermochaetoides thermophila additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Trichoderma koningii additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Aspergillus aculeatus additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Acetivibrio thermocellus additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Prevotella ruminicola additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Cellulomonas fimi additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Rasamsonia emersonii additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Lenzites trabea additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Acetivibrio cellulolyticus additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Streptomyces reticuli additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Ruminiclostridium cellobioparum additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Ruminiclostridium josui additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Sporocytophaga myxococcoides additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Robillarda sp. additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Bacillus cellulyticus K-12 additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Cellulomonas sp. additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Irpex lacteus additional information glucose + cellobiose + cellotriose + cellotetraose ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Irpex lacteus additional information various cellooligosaccharides ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Bacillus subtilis additional information glucose + cellobiose ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Robillarda sp. additional information mainly glucose with small amounts of cellobiose and cellotriose ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Acetivibrio thermocellus additional information endoglucanase 1b: cellotriose, 45%, cellotetraose, 31%, cellobiose, 17%, and cellopentaose, 8%. Endoglucanase 2: cellotriose, 50%, and cellotetraose, 30% ? 259795 3.2.1.4 carboxymethylcellulose + H2O catalytic domain of endoglucanase G Fibrobacter succinogenes additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O hydrolyzed by cellulase I and III, no hydrolysis with cellulase II Thermoascus aurantiacus additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O endoglucanase E1 exhibits higher activity Thermobifida fusca additional information endoglucanase E2 produces glucose, cellobiose, cellotriose and higher oligosaccharides. The major end product produced by endoglucanase E1 is cellobiose and small amounts of glucose ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Bacillus sp. (in: Bacteria) No. 1139 additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Thermothelomyces heterothallicus D-14 additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Streptomyces lividans 1326 additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Robillarda sp. Y-20 additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Robillarda sp. Y-20 additional information mainly glucose with small amounts of cellobiose and cellotriose ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Aspergillus niger IFO31125 additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Bacillus sp. (in: Bacteria) N-4 additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Ruminococcus albus F40 additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Aspergillus terreus GN1 additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Fibrobacter succinogenes S85 additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O catalytic domain of endoglucanase G Fibrobacter succinogenes S85 additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Niallia circulans F-2 additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Trichoderma reesei QM6a additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Rasamsonia emersonii CBS 814.70 additional information - ? 259795 3.2.1.4 carboxymethylcellulose + H2O - Saccharolobus solfataricus cellobiose + ? - ? 259929 3.2.1.4 carboxymethylcellulose + H2O - Saccharolobus solfataricus P2 cellobiose + ? - ? 259929 3.2.1.4 carboxymethylcellulose + H2O - Lysinibacillus sphaericus carboxymethyl glucose + cellobiose + cellotriose - ? 372620 3.2.1.4 carboxymethylcellulose + H2O - Lysinibacillus sphaericus JS1 carboxymethyl glucose + cellobiose + cellotriose - ? 372620 3.2.1.4 carboxymethylcellulose + H2O - Saccharolobus solfataricus cellobiose + cello-oligomer - ? 400615 3.2.1.4 carboxymethylcellulose + H2O - Gecarcoidea natalis short glucose oligomers + cellobiose + glucose - ? 404607 3.2.1.4 carboxymethylcellulose + H2O - Gecarcoidea natalis Pocock 1888 short glucose oligomers + cellobiose + glucose - ? 404607 3.2.1.4 carboxymethylcellulose + H2O endo-beta-1,4-glucanase activity. The enzyme shows cellulase and xylanase activity Saccharolobus solfataricus cellobiose + cellooligomers - ? 423107 3.2.1.4 carboxymethylcellulose + H2O - Cryptopygus antarcticus cellobiose + cellotriose + cellotetraose - ? 443264 3.2.1.4 carboxymethylcellulose + H2O - Thermothelomyces thermophilus cellobiose + cellotriose + cellotetraose + cellopentaose - ? 443265 3.2.1.4 carboxymethylcellulose + H2O - Thermothelomyces thermophilus BJA cellobiose + cellotriose + cellotetraose + cellopentaose - ? 443265 3.2.1.4 carboxymethylpachyman + H2O cellulase I Thermoascus aurantiacus ? - ? 259805 3.2.1.4 carob bean gum + H2O - Rasamsonia emersonii ? - ? 430016 3.2.1.4 cello-oligosaccharide + H2O insoluble cello-oligosaccharide average degree of polymerization 34 and soluble cello-oligosaccharides longer than cellotriose Psacothea hilaris ? - ? 372629 3.2.1.4 cellobiose + H2O - Gracilibacillus sp. 2 D-glucose - ? 94496 3.2.1.4 cellobiose + H2O - Trichoderma atroviride 2 D-glucose - ? 94496 3.2.1.4 cellobiose + H2O - Actinomyces sp. 40 2 D-glucose 26% of the activity with carboxymethyl cellulose ? 94496 3.2.1.4 cellobiose + H2O - Gracilibacillus sp. SK1 2 D-glucose - ? 94496 3.2.1.4 cellobiose + H2O - Actinomyces sp. 40 Korean Native Goat 40 2 D-glucose 26% of the activity with carboxymethyl cellulose ? 94496 3.2.1.4 cellobiose + H2O - Thermobifida fusca ? - ? 372639 3.2.1.4 cellobiose + H2O - Coptotermes formosanus ? - ? 372639 3.2.1.4 cellobiose + H2O - Cryptococcus sp. S-2 ? - ? 372639 3.2.1.4 cellobiose + H2O - Aspergillus niger ? 9.8% of the activity with carboxymethyl cellulose ? 372639 3.2.1.4 cellobiose + H2O 8.6% of the activity with carboxymethylcellulose Bacillus amyloliquefaciens ? - ? 372639 3.2.1.4 cellobiose + H2O 25% of the activity with carboxymethyl cellulose Ganoderma lucidum ? - ? 372639 3.2.1.4 cellobiose + H2O - Aspergillus niger HO ? 9.8% of the activity with carboxymethyl cellulose ? 372639 3.2.1.4 cellobioside + H2O about 2% of the activity with barley beta-glucan Orpinomyces joyonii ? - ? 372644 3.2.1.4 cellodextrin + H2O - Komagataeibacter xylinus ? - ? 259814 3.2.1.4 cellodextrin + H2O cellulase A, B, and C Pseudomonas fluorescens ? - ? 259814 3.2.1.4 celloheptaose + H2O - Robillarda sp. ? - ? 94638 3.2.1.4 celloheptaose + H2O - Robillarda sp. Y-20 ? - ? 94638 3.2.1.4 cellohexaitol + H2O - Acetivibrio thermocellus ? - ? 391602 3.2.1.4 cellohexaose + 5 H2O - uncultured bacterium 6 D-glucose - ? 370056 3.2.1.4 cellohexaose + H2O - Gloeophyllum trabeum D-glucose + ? - ? 94637 3.2.1.4 cellohexaose + H2O - Aspergillus niger additional information - ? 259812 3.2.1.4 cellohexaose + H2O - Ruminococcus albus additional information - ? 259812 3.2.1.4 cellohexaose + H2O - Trichoderma viride additional information - ? 259812 3.2.1.4 cellohexaose + H2O - Ruminiclostridium josui additional information - ? 259812 3.2.1.4 cellohexaose + H2O - Robillarda sp. additional information - ? 259812 3.2.1.4 cellohexaose + H2O - Niallia circulans additional information cellotriose + cellobiose + cellotetraose ? 259812 3.2.1.4 cellohexaose + H2O - Ruminiclostridium josui additional information cellotriose + cellobiose + cellotetraose ? 259812 3.2.1.4 cellohexaose + H2O - Dolabella auricularia additional information cellobiose + cellotetraose ? 259812 3.2.1.4 cellohexaose + H2O - Bacillus cellulyticus K-12 additional information cellobiose + cellotetraose ? 259812 3.2.1.4 cellohexaose + H2O - Athelia rolfsii additional information cellotriose + cellobiose + cellotriose ? 259812 3.2.1.4 cellohexaose + H2O - Aspergillus niger additional information cellotriose + glucose + cellobiose + cellotetraose ? 259812 3.2.1.4 cellohexaose + H2O - Pisum sativum additional information cellotriose is formed and then slowly degraded ? 259812 3.2.1.4 cellohexaose + H2O cellulase A, B, and C Pseudomonas fluorescens additional information - ? 259812 3.2.1.4 cellohexaose + H2O - Robillarda sp. Y-20 additional information - ? 259812 3.2.1.4 cellohexaose + H2O - Rhizopus arrhizus ? - ? 372645 3.2.1.4 cellohexaose + H2O - Acetivibrio thermocellus ? - ? 372645 3.2.1.4 cellohexaose + H2O - Clostridium cellulovorans ? - ? 372645 3.2.1.4 cellohexaose + H2O - Pyrococcus furiosus ? - ? 372645 3.2.1.4 cellohexaose + H2O the enzyme hydrolyzes cellotetraose, cellopentaose, and cellohexaose at comparable rates Thermochaetoides thermophila ? - ? 372645 3.2.1.4 cellohexaose + H2O - Niallia circulans 2 cellotriose + small amounts of cellobiose and cellotriose ? 372647 3.2.1.4 cellohexaose + H2O - Niallia circulans KSM-N257 2 cellotriose + small amounts of cellobiose and cellotriose ? 372647 3.2.1.4 cellohexaose + H2O - Komagataeibacter xylinus cellobiose + cellotriose + cellotetraose - ? 381633 3.2.1.4 cellohexaose + H2O - Thermothelomyces thermophilus cellobiose + cellotriose + cellotetraose - ? 381633 3.2.1.4 cellohexaose + H2O - Bellamya chinensis laeta cellobiose + cellotriose + cellotetraose - ? 381633 3.2.1.4 cellohexaose + H2O - Bellamya chinensis laeta UM-2014 cellobiose + cellotriose + cellotetraose - ? 381633 3.2.1.4 cellohexaose + H2O major product is cellotriose, minor products are cellobiose and cellotetraose Lysobacter sp. cellotriose + cellotetraose + cellobiose - ? 391603 3.2.1.4 cellohexaose + H2O major product is cellotriose, minor products are cellobiose and cellotetraose Lysobacter sp. IB-9374 cellotriose + cellotetraose + cellobiose - ? 391603 3.2.1.4 cellohexaose + H2O - Euphausia pacifica cellotriose + cellobiose + D-glucose - ? 430025 3.2.1.4 cellohexaose + H2O - Saccharolobus solfataricus cellobiose + cellotriose - ? 437265 3.2.1.4 cellohexaose + H2O - Saccharolobus solfataricus DSM 1617 cellobiose + cellotriose - ? 437265 3.2.1.4 cellohexaose + H2O - Cryptopygus antarcticus cellotetraose + cellobiose - ? 443275 3.2.1.4 cellohexaose + H2O - Cellulomonas fimi cellotriose + cellobiose - ? 447283 3.2.1.4 cellohexaose + H2O cellobiose is the main product Irpex lacteus cellobiose + cellotriose + cellotetraose + cellulose + cellopentaose - ? 452777 3.2.1.4 cellooligosaccharide + H2O - Thermobifida fusca ? - ? 372648 3.2.1.4 cellooligosaccharide + H2O - Populus tremula x Populus tremuloides ? - ? 372648 3.2.1.4 cellooligosaccharide + H2O insolvent cellooligosaccharide 33 Rhizopus arrhizus ? - ? 372648 3.2.1.4 cellooligosaccharides + H2O random cleavage, slow reaction rates for cellooligosaccharides smaller than G5 Clostridium cellulovorans ? - ? 391605 3.2.1.4 cellopentaose transfer reaction Robillarda sp. cellohexaose + celloheptaose - ? 259811 3.2.1.4 cellopentaose + 4 H2O - uncultured bacterium 5 D-glucose - ? 370057 3.2.1.4 cellopentaose + H2O - Gloeophyllum trabeum D-glucose + ? - ? 94636 3.2.1.4 cellopentaose + H2O - Aspergillus niger additional information - ? 259810 3.2.1.4 cellopentaose + H2O - Pisum sativum additional information - ? 259810 3.2.1.4 cellopentaose + H2O - Ruminococcus albus additional information - ? 259810 3.2.1.4 cellopentaose + H2O - Komagataeibacter xylinus additional information - ? 259810 3.2.1.4 cellopentaose + H2O - Trichoderma viride additional information - ? 259810 3.2.1.4 cellopentaose + H2O - Trichoderma koningii additional information - ? 259810 3.2.1.4 cellopentaose + H2O - Acetivibrio thermocellus additional information - ? 259810 3.2.1.4 cellopentaose + H2O - Robillarda sp. additional information - ? 259810 3.2.1.4 cellopentaose + H2O - Bacillus cellulyticus K-12 additional information - ? 259810 3.2.1.4 cellopentaose + H2O - Niallia circulans additional information cellobiose + cellotriose ? 259810 3.2.1.4 cellopentaose + H2O - Ruminiclostridium josui additional information cellobiose + cellotriose ? 259810 3.2.1.4 cellopentaose + H2O - Dolabella auricularia additional information cellotetraose + glucose ? 259810 3.2.1.4 cellopentaose + H2O - Athelia rolfsii additional information cellobiose + cellotriose + glucose ? 259810 3.2.1.4 cellopentaose + H2O enzyme form EGB and EGC Acetivibrio thermocellus additional information - ? 259810 3.2.1.4 cellopentaose + H2O cellulase A, B, and C Pseudomonas fluorescens additional information - ? 259810 3.2.1.4 cellopentaose + H2O beta-configuration of the anomeric carbon atoms is retained Trichoderma viride additional information - ? 259810 3.2.1.4 cellopentaose + H2O hydrolyzed by endo-beta-1,4-glucanase component YEG1 and YEG2 Reticulitermes speratus additional information cellobiose + cellotriose ? 259810 3.2.1.4 cellopentaose + H2O - Robillarda sp. Y-20 additional information - ? 259810 3.2.1.4 cellopentaose + H2O - Ruminococcus albus F40 additional information - ? 259810 3.2.1.4 cellopentaose + H2O - Niallia circulans F-2 additional information cellobiose + cellotriose ? 259810 3.2.1.4 cellopentaose + H2O - Rhizopus arrhizus ? - ? 372650 3.2.1.4 cellopentaose + H2O - Acetivibrio thermocellus ? - ? 372650 3.2.1.4 cellopentaose + H2O - Xylella fastidiosa ? - ? 372650 3.2.1.4 cellopentaose + H2O - Clostridium cellulovorans ? - ? 372650 3.2.1.4 cellopentaose + H2O - Pyrococcus furiosus ? - ? 372650 3.2.1.4 cellopentaose + H2O minimal length substrate Neurospora crassa ? - ? 372650 3.2.1.4 cellopentaose + H2O the enzyme hydrolyzes cellotetraose, cellopentaose, and cellohexaose at comparable rates Thermochaetoides thermophila ? - ? 372650 3.2.1.4 cellopentaose + H2O minimal length substrate Neurospora crassa DSM 1257 ? - ? 372650 3.2.1.4 cellopentaose + H2O - Niallia circulans cellobiose + cellotriose - ? 372651 3.2.1.4 cellopentaose + H2O - uncultured microorganism cellobiose + cellotriose - ? 372651 3.2.1.4 cellopentaose + H2O - Saccharolobus solfataricus cellobiose + cellotriose - ? 372651 3.2.1.4 cellopentaose + H2O - Thermothelomyces thermophilus cellobiose + cellotriose - ? 372651 3.2.1.4 cellopentaose + H2O - Alicyclobacillus vulcanalis cellobiose + cellotriose - ? 372651 3.2.1.4 cellopentaose + H2O - Niallia circulans KSM-N257 cellobiose + cellotriose - ? 372651 3.2.1.4 cellopentaose + H2O - Saccharolobus solfataricus P2 cellobiose + cellotriose - ? 372651 3.2.1.4 cellopentaose + H2O - Saccharolobus solfataricus DSM 1617 cellobiose + cellotriose - ? 372651 3.2.1.4 cellopentaose + H2O - Ruminiclostridium cellulolyticum cellotetraose + glucose - ? 372654 3.2.1.4 cellopentaose + H2O - unidentified microorganism cellobiose + glucose - ? 391607 3.2.1.4 cellopentaose + H2O - Cryptopygus antarcticus cellotriose + cellobiose - ? 391608 3.2.1.4 cellopentaose + H2O - Cellulomonas fimi cellotriose + cellobiose - ? 391608 3.2.1.4 cellopentaose + H2O - Cellulomonas fimi NBRC 15513 cellotriose + cellobiose - ? 391608 3.2.1.4 cellopentaose + H2O - Cellulomonas fimi NCIMB 8980 cellotriose + cellobiose - ? 391608 3.2.1.4 cellopentaose + H2O - Cellulomonas fimi NCTC 7547 cellotriose + cellobiose - ? 391608 3.2.1.4 cellopentaose + H2O - Cellulomonas fimi ATCC 484 cellotriose + cellobiose - ? 391608 3.2.1.4 cellopentaose + H2O - Cellulomonas fimi JCM 1341 cellotriose + cellobiose - ? 391608 3.2.1.4 cellopentaose + H2O - Cellulomonas fimi DSM 20113 cellotriose + cellobiose - ? 391608 3.2.1.4 cellopentaose + H2O - Euphausia pacifica cellotriose + cellobiose + D-glucose - ? 430026 3.2.1.4 cellopentaose + H2O cellobiose is the main product Irpex lacteus cellobiose + cellotriose + cellotetraose + cellulose - ? 452778 3.2.1.4 cellopentaosyl sorbitol + H2O cellulase A, B, and C Pseudomonas fluorescens ? - ? 259823 3.2.1.4 cellotetraose transfer reaction Robillarda sp. cellopentaose + cellohexaose - ? 259809 3.2.1.4 cellotetraose transfer reaction Robillarda sp. Y-20 cellopentaose + cellohexaose - ? 259809 3.2.1.4 cellotetraose + 3 H2O - uncultured bacterium 4 D-glucose - ? 370058 3.2.1.4 cellotetraose + H2O - Aspergillus niger 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Pisum sativum 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Bacillus sp. (in: Bacteria) 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Niallia circulans 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Ruminococcus albus 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Trichoderma viride 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Dolabella auricularia 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Athelia rolfsii 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Trichoderma koningii 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Acetivibrio thermocellus 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Ruminiclostridium josui 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Robillarda sp. 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Reticulitermes speratus 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - uncultured microorganism 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Saccharolobus solfataricus 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Alicyclobacillus vulcanalis 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Cryptopygus antarcticus 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Cellulomonas fimi 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Robillarda sp. 2 cellobiose predominant product ? 259808 3.2.1.4 cellotetraose + H2O - Aspergillus niger 2 cellobiose + glucose + cellotriose ? 259808 3.2.1.4 cellotetraose + H2O - Komagataeibacter xylinus 2 cellobiose + glucose + cellotriose ? 259808 3.2.1.4 cellotetraose + H2O - Irpex lacteus 2 cellobiose + glucose + cellotriose ? 259808 3.2.1.4 cellotetraose + H2O - Bacillus sp. (in: Bacteria) 2 cellobiose glucose, 15%, cellobiose, 64%, cellotriose, 14% and cellotetraose, 3.3% ? 259808 3.2.1.4 cellotetraose + H2O effectively hydrolyzed by endoglucanase E1, limited hydrolysis by endoglucanase E2 Thermobifida fusca 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O enzyme form EGB and EGC Acetivibrio thermocellus 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O cellulase A, B, and C Pseudomonas fluorescens 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O minimal substrate length, cellotriose and cellobiose are not hydrolyzed Saccharolobus solfataricus 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Cellulomonas fimi NBRC 15513 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Bacillus sp. (in: Bacteria) No. 1139 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Bacillus sp. (in: Bacteria) No. 1139 2 cellobiose glucose, 15%, cellobiose, 64%, cellotriose, 14% and cellotetraose, 3.3% ? 259808 3.2.1.4 cellotetraose + H2O - Cellulomonas fimi NCIMB 8980 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O minimal substrate length, cellotriose and cellobiose are not hydrolyzed Saccharolobus solfataricus P2 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Cellulomonas fimi NCTC 7547 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Robillarda sp. Y-20 2 cellobiose predominant product ? 259808 3.2.1.4 cellotetraose + H2O - Robillarda sp. Y-20 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Bacillus sp. (in: Bacteria) N-4 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Ruminococcus albus F40 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Cellulomonas fimi ATCC 484 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Cellulomonas fimi JCM 1341 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Niallia circulans F-2 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Saccharolobus solfataricus DSM 1617 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Cellulomonas fimi DSM 20113 2 cellobiose - ? 259808 3.2.1.4 cellotetraose + H2O - Thermobifida fusca ? - ? 372657 3.2.1.4 cellotetraose + H2O - Xylella fastidiosa ? - ? 372657 3.2.1.4 cellotetraose + H2O - Clostridium cellulovorans ? - ? 372657 3.2.1.4 cellotetraose + H2O - Coptotermes formosanus ? - ? 372657 3.2.1.4 cellotetraose + H2O the enzyme hydrolyzes cellotetraose, cellopentaose, and cellohexaose at comparable rates Thermochaetoides thermophila ? - ? 372657 3.2.1.4 cellotetraose + H2O - Euphausia pacifica cellotriose + cellobiose + D-glucose - ? 430027 3.2.1.4 cellotetraose + H2O - Gloeophyllum trabeum D-glucose + cellobiose + cellotriose - ? 447285 3.2.1.4 cellotetraosyl sorbitol + H2O cellulase A, B, and C Pseudomonas fluorescens ? - ? 259822 3.2.1.4 cellotriose transfer reaction Robillarda sp. cellopentaose + cellotetraose - ? 259807 3.2.1.4 cellotriose transfer reaction Robillarda sp. Y-20 cellopentaose + cellotetraose - ? 259807 3.2.1.4 cellotriose + 2 H2O - uncultured bacterium 3 D-glucose - ? 370059 3.2.1.4 cellotriose + H2O - Thermobifida fusca ? - ? 94515 3.2.1.4 cellotriose + H2O - Coptotermes formosanus ? - ? 94515 3.2.1.4 cellotriose + H2O - Aspergillus niger D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O - Pisum sativum D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O - Bacillus sp. (in: Bacteria) D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O - Trichoderma viride D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O - Gloeophyllum trabeum D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O - Robillarda sp. D-glucose + cellobiose predominant product ? 94634 3.2.1.4 cellotriose + H2O - Bacillus sp. (in: Bacteria) D-glucose + cellobiose a mixture of glucose, 5.5%, cellobiose, 55%, cellotriose, 34%, and traces of cellotetraose ? 94634 3.2.1.4 cellotriose + H2O - Aspergillus niger D-glucose + cellobiose + glucose ? 94634 3.2.1.4 cellotriose + H2O - Irpex lacteus D-glucose + cellobiose + glucose ? 94634 3.2.1.4 cellotriose + H2O no activity Ruminiclostridium josui D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O no reaction Niallia circulans D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O no reaction Ruminiclostridium josui D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O no hydrolysis Acetivibrio thermocellus D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O enzyme form EGC Acetivibrio thermocellus D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O effectively hydrolyzed by endoglucanase E1, limited hydrolysis by endoglucanase E2 Thermobifida fusca D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O hydrolyzed by endoglucanase E1, no activity with endoglucanase E2 Thermobifida fusca D-glucose + cellobiose + glucose, endoglucanase E1 ? 94634 3.2.1.4 cellotriose + H2O cellulase C Pseudomonas fluorescens D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O negligibly hydrolyzed Ruminococcus albus D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O hydrolyzed by endo-beta-1,4-glucanase component YEG2, no hydrolysis with endo-beta-1,4-glucanase component YEG1 Reticulitermes speratus D-glucose + cellobiose + glucose ? 94634 3.2.1.4 cellotriose + H2O slightly degraded uncultured microorganism D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O - Bacillus sp. (in: Bacteria) No. 1139 D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O - Bacillus sp. (in: Bacteria) No. 1139 D-glucose + cellobiose a mixture of glucose, 5.5%, cellobiose, 55%, cellotriose, 34%, and traces of cellotetraose ? 94634 3.2.1.4 cellotriose + H2O - Robillarda sp. Y-20 D-glucose + cellobiose predominant product ? 94634 3.2.1.4 cellotriose + H2O - Bacillus sp. (in: Bacteria) N-4 D-glucose + cellobiose - ? 94634 3.2.1.4 cellotriose + H2O no reaction Niallia circulans F-2 D-glucose + cellobiose - ? 94634 3.2.1.4 cellotrioside + H2O 7.4% of the activity with barley beta-glucan Orpinomyces joyonii ? - ? 372664 3.2.1.4 cellulose + H2O - Phaseolus vulgaris ? - ? 94577 3.2.1.4 cellulose + H2O - Bacillus subtilis ? - ? 94577 3.2.1.4 cellulose + H2O - Aspergillus niger ? - ? 94577 3.2.1.4 cellulose + H2O - Bacillus sp. (in: Bacteria) ? - ? 94577 3.2.1.4 cellulose + H2O - Bacillus pumilus ? - ? 94577 3.2.1.4 cellulose + H2O - Komagataeibacter xylinus ? - ? 94577 3.2.1.4 cellulose + H2O - Clonostachys rosea ? - ? 94577 3.2.1.4 cellulose + H2O - Catharanthus roseus ? - ? 94577 3.2.1.4 cellulose + H2O - Trichoderma reesei ? - ? 94577 3.2.1.4 cellulose + H2O - Trichoderma koningii ? - ? 94577 3.2.1.4 cellulose + H2O - Trichocladium griseum ? - ? 94577 3.2.1.4 cellulose + H2O - Sporotrichum pulverulentum ? - ? 94577 3.2.1.4 cellulose + H2O - Cellulomonas fimi ? - ? 94577 3.2.1.4 cellulose + H2O - Talaromyces pinophilus ? - ? 94577 3.2.1.4 cellulose + H2O - Streptomyces rochei ? - ? 94577 3.2.1.4 cellulose + H2O - Brevibacillus sp. ? - ? 94577 3.2.1.4 cellulose + H2O - Fusarium solani-melongenae ? - ? 94577 3.2.1.4 cellulose + H2O - Acetivibrio cellulolyticus ? - ? 94577 3.2.1.4 cellulose + H2O - Geobacillus sp. ? - ? 94577 3.2.1.4 cellulose + H2O - Marinobacter sp. ? - ? 94577 3.2.1.4 cellulose + H2O - Trichoderma citrinoviride ? - ? 94577 3.2.1.4 cellulose + H2O - Haloarcula sp. ? - ? 94577 3.2.1.4 cellulose + H2O - Corbicula japonica ? - ? 94577 3.2.1.4 cellulose + H2O - Saccharolobus solfataricus ? - ? 94577 3.2.1.4 cellulose + H2O - Melanocarpus albomyces ? - ? 94577 3.2.1.4 cellulose + H2O - Populus alba ? - ? 94577 3.2.1.4 cellulose + H2O - Pyrococcus horikoshii ? - ? 94577 3.2.1.4 cellulose + H2O - Nilaparvata lugens ? - ? 94577 3.2.1.4 cellulose + H2O - Reticulitermes speratus ? crystalline cellulose is hydrolyzed to glucose and cellobiose ? 94577 3.2.1.4 cellulose + H2O Walseth cellulose Athelia rolfsii ? - ? 94577 3.2.1.4 cellulose + H2O Walseth cellulose Talaromyces funiculosus ? - ? 94577 3.2.1.4 cellulose + H2O Walseth cellulose Irpex lacteus ? products from Walseth cellulose: glucose + cellobiose + cellotriose + cellotetraose ? 94577 3.2.1.4 cellulose + H2O Walseth cellulose Talaromyces funiculosus ? mainly glucose, cellobiose and traces of cellotriose ? 94577 3.2.1.4 cellulose + H2O endoglucanase E1 exhibits higher activity Thermobifida fusca ? - ? 94577 3.2.1.4 cellulose + H2O endoglucanase I shows very low activity with acid-swollen cellulose Acetivibrio thermocellus ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Aspergillus niger ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Populus x canadensis ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Ruminococcus albus ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Trichoderma viride ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Thermobifida fusca ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Dolabella auricularia ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Thermothelomyces heterothallicus ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Talaromyces funiculosus ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Trichoderma koningii ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Aspergillus aculeatus ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Acetivibrio thermocellus ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Sporotrichum pulverulentum ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Rasamsonia emersonii ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Ruminococcus albus ? large amounts of cellotriose and a small amount of cellotetraose ? 94577 3.2.1.4 cellulose + H2O acid-swollen Dickeya chrysanthemi ? major end product are cellobiose and cellotriose, endoglucanase Z ? 94577 3.2.1.4 cellulose + H2O swollen cellulose Fibrobacter succinogenes ? a mixture of cellooligosaccharides ? 94577 3.2.1.4 cellulose + H2O no degradation of crystalline cellulose Trichoderma reesei ? - ? 94577 3.2.1.4 cellulose + H2O Walseth cellulose shows 1.1% of the activity with carboxymethylcellulose Acetivibrio thermocellus ? - ? 94577 3.2.1.4 cellulose + H2O amorphous cellulose and ball-milled cellulose Prevotella ruminicola ? - ? 94577 3.2.1.4 cellulose + H2O little ability to hydrolyze ordered cellulose Acetivibrio thermocellus ? - ? 94577 3.2.1.4 cellulose + H2O enzyme form EGA and EGD Acetivibrio thermocellus ? - ? 94577 3.2.1.4 cellulose + H2O amorphous cellulose Trichoderma viride ? - ? 94577 3.2.1.4 cellulose + H2O amorphous cellulose Trichoderma koningii ? - ? 94577 3.2.1.4 cellulose + H2O amorphous cellulose Thermobifida fusca ? endoglucanase E1 produces mainly cellobiose and a trace of glucose, endoglucanase E2 produces mainly cellotriose and higher oligomers with some cellobiose and a trace of glucose ? 94577 3.2.1.4 cellulose + H2O partial degradation of native cellulose Thermoascus aurantiacus ? - ? 94577 3.2.1.4 cellulose + H2O no hydrolysis of native cellulose Sporocytophaga myxococcoides ? - ? 94577 3.2.1.4 cellulose + H2O amorphous cellulose (relative activity 15.2%), filter paper (shows poor growth and enzyme production, relative activity 3.1%), cotton wool (relative activity 4.3%) Bacillus sp. (in: Bacteria) ? - ? 94577 3.2.1.4 cellulose + H2O Avicel or coastal Bermuda grass Orpinomyces sp. PC-2 ? - ? 94577 3.2.1.4 cellulose + H2O crystalline and amorphous cellulose Melanocarpus albomyces ? - ? 94577 3.2.1.4 cellulose + H2O the catalytic domains of Cel6A, Cel6B, Cel48A, Cel5A, and Cel9A only show very weak binding to bacterial cellulose Thermobifida fusca ? - ? 94577 3.2.1.4 cellulose + H2O hydrolysis of Solka floc, Avicel, CMC, waste paper, and waste wood refiner Talaromyces pinophilus ? - ? 94577 3.2.1.4 cellulose + H2O the family 1 carbohydrate-binding module, CBM, mediates the interaction between enzyme and crystalline cellulose surface via residues Y5, Q7, N29, and Y32, thus CBM is responsible for anchoring the enzyme at discrete points along a cellulose chain to aid in both recognizing cellulose chain ends for initial attachment to cellulose as well as aid in enzymatic catalysis by diffusing between stable wells on a length scale commensurate with the catalytic, processive cycle of Cel7A during cellulose hydrolysis, molecular-level mechanisms of recognition and interaction, overview Trichoderma reesei ? - ? 94577 3.2.1.4 cellulose + H2O activity is evaluated on 13 insoluble celluloses characterized for crystallinity and crystal width (by X-ray diffraction), wet porosity (by thermoporometry), and particle size (by light scattering). Looser crystalline packing increases the lengths of released cello-oligomers as well as the maximum endoglucanase specific activity (kcat) Pyrococcus furiosus ? - ? 94577 3.2.1.4 cellulose + H2O maximal activity soluble cellulosic substrate (CMC-Na salt), indicating that the crude enzyme has endoglucanase activity Haloarcula sp. ? - ? 94577 3.2.1.4 cellulose + H2O pretreatment of cellulose with ionic liquids such as 1-butyl-3-methylimidazolium chloride, 1-methylimidazolium chloride, and tris-(2-hydroxyethyl)-methylammonium methylsulfate results in more rapid conversion to glucose than hydrolysis with cellulose that is not pretreated Aspergillus niger ? - ? 94577 3.2.1.4 cellulose + H2O filter paper, 18% activity compared to carboxymethyl cellulose Rhodococcus sp. ? - ? 94577 3.2.1.4 cellulose + H2O - Haloarcula sp. G10 ? - ? 94577 3.2.1.4 cellulose + H2O maximal activity soluble cellulosic substrate (CMC-Na salt), indicating that the crude enzyme has endoglucanase activity Haloarcula sp. LLSG7 ? - ? 94577 3.2.1.4 cellulose + H2O - Brevibacillus sp. JXL ? - ? 94577 3.2.1.4 cellulose + H2O - Pyrococcus horikoshii DSM 12428 ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Aspergillus niger IFO31125 ? - ? 94577 3.2.1.4 cellulose + H2O - Streptomyces rochei A2 ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Ruminococcus albus F40 ? - ? 94577 3.2.1.4 cellulose + H2O - Marinobacter sp. MSI032 ? - ? 94577 3.2.1.4 cellulose + H2O amorphous cellulose (relative activity 15.2%), filter paper (shows poor growth and enzyme production, relative activity 3.1%), cotton wool (relative activity 4.3%) Bacillus sp. (in: Bacteria) CTP-09 ? - ? 94577 3.2.1.4 cellulose + H2O - Talaromyces pinophilus CF-2612 ? - ? 94577 3.2.1.4 cellulose + H2O swollen cellulose Fibrobacter succinogenes S85 ? a mixture of cellooligosaccharides ? 94577 3.2.1.4 cellulose + H2O - Bacillus sp. (in: Bacteria) DUSELR13 ? - ? 94577 3.2.1.4 cellulose + H2O - Bacillus subtilis BME-15 ? - ? 94577 3.2.1.4 cellulose + H2O - Bacillus subtilis JA18 ? - ? 94577 3.2.1.4 cellulose + H2O no degradation of crystalline cellulose Trichoderma reesei QM6a ? - ? 94577 3.2.1.4 cellulose + H2O - Geobacillus sp. WSUCFI ? - ? 94577 3.2.1.4 cellulose + H2O - Bacillus subtilis LN ? - ? 94577 3.2.1.4 cellulose + H2O acid-swollen Rasamsonia emersonii CBS 814.70 ? - ? 94577 3.2.1.4 cellulose + H2O - Trichoderma reesei D-glucose + ? - ? 94659 3.2.1.4 cellulose + H2O - Fervidobacterium nodosum Rt17-B1 D-glucose + ? - ? 94659 3.2.1.4 cellulose + H2O - Caldicellulosiruptor saccharolyticus D-glucose + ? enzyme is able to degrade crystalline cellulose to glucose ? 94659 3.2.1.4 cellulose + H2O at 90°C, pH 4 by an enzyme mix consisting of SSO1354 and additional glycosyl hydrolases Saccharolobus solfataricus D-glucose + ? - ? 94659 3.2.1.4 cellulose + H2O enzyme FnCel5A catalyzes the hydrolysis of cellulose to glucose Fervidobacterium nodosum D-glucose + ? - ? 94659 3.2.1.4 cellulose + H2O enzyme FnCel5A catalyzes the hydrolysis of cellulose to glucose Fervidobacterium nodosum ATCC 35602 D-glucose + ? - ? 94659 3.2.1.4 cellulose + H2O enzyme FnCel5A catalyzes the hydrolysis of cellulose to glucose Fervidobacterium nodosum DSM 5306 D-glucose + ? - ? 94659 3.2.1.4 cellulose + H2O - Pyrococcus horikoshii cellooligosaccharide - ? 370194 3.2.1.4 cellulose + H2O inducible enzyme Sporocytophaga myxococcoides cellooligosaccharide - ? 370194 3.2.1.4 cellulose + H2O induced by carboxymethylcellulose, non-constitutive synthesis Thermomyces lanuginosus cellooligosaccharide - ? 370194 3.2.1.4 cellulose + H2O enzyme is involved in degradation of cellulose Sporotrichum pulverulentum cellooligosaccharide - ? 370194 3.2.1.4 cellulose + H2O enzyme plays an important role in the carbon cycle Thermothelomyces heterothallicus cellooligosaccharide - ? 370194 3.2.1.4 cellulose + H2O in bean the 9.5 cellulase appears to function in the shedding of various organs such as fruits, flowers, and leaves. The acid cellulases function in loosening the cellulose fibrils of the cell wall to allow expansion and growth Phaseolus vulgaris cellooligosaccharide - ? 370194 3.2.1.4 cellulose + H2O the enzyme plays an important role in maintaining the carbon balance in nature Aspergillus niger cellooligosaccharide - ? 370194 3.2.1.4 cellulose + H2O enzyme plays an important role in the carbon cycle Thermothelomyces heterothallicus D-14 cellooligosaccharide - ? 370194 3.2.1.4 cellulose + H2O - Reticulitermes speratus cellobiose + ? - ? 372665 3.2.1.4 cellulose + H2O the secreted protein enables Sulfolobus solfataricus to use cellulose as an external carbon source Saccharolobus solfataricus cellobiose + ? - ? 372665 3.2.1.4 cellulose + H2O the substrate position is fixed by the alignment of one cellobiose unit between the two aromatic amino acid residues at subsites +1 and +2. During the enzyme reaction, the glucose structure of cellulose substrates is distorted at subsite -1, and the beta-1,4-glucoside bond between glucose moieties is twisted between subsites -1 and +1. Subsite -2 specifically recognizes the glucose residue, but recognition by subsites +1 and +2 is loose during the enzyme reaction. Analysis of the enzyme-substrate structure suggests that an incoming water molecule, essential for hydrolysis during the retention process, might be introduced to the cleavage position after the cellobiose product at subsites +1 and +2 is released from the active site Pyrococcus horikoshii cellobiose + ? - ? 372665 3.2.1.4 cellulose + H2O the secreted protein enables Sulfolobus solfataricus to use cellulose as an external carbon source Saccharolobus solfataricus P2 cellobiose + ? - ? 372665 3.2.1.4 cellulose + H2O the substrate position is fixed by the alignment of one cellobiose unit between the two aromatic amino acid residues at subsites +1 and +2. During the enzyme reaction, the glucose structure of cellulose substrates is distorted at subsite -1, and the beta-1,4-glucoside bond between glucose moieties is twisted between subsites -1 and +1. Subsite -2 specifically recognizes the glucose residue, but recognition by subsites +1 and +2 is loose during the enzyme reaction. Analysis of the enzyme-substrate structure suggests that an incoming water molecule, essential for hydrolysis during the retention process, might be introduced to the cleavage position after the cellobiose product at subsites +1 and +2 is released from the active site Pyrococcus horikoshii OT-3 cellobiose + ? - ? 372665 3.2.1.4 cellulose + H2O - Trichoderma reesei cellobiose + glucose - ? 400642 3.2.1.4 cellulose + H2O - Thermochaetoides thermophila cellobiose + cellotriose + cellotetraose + cellopentaose - ? 437268 3.2.1.4 cellulose from Smallanthus sonchifolius - Trichoderma reesei D-glucose + ? - ? 452775 3.2.1.4 cellulose from yam - Trichoderma reesei D-glucose + ? - ? 452776 3.2.1.4 chitotetraose + H2O - Thermobifida fusca ? - ? 364722 3.2.1.4 chitotriose + H2O - Thermobifida fusca ? - ? 381653 3.2.1.4 CM cellulose + H2O - Cryptococcus sp. S-2 ? - ? 400687 3.2.1.4 corn stover + H2O - Gracilibacillus sp. ? - ? 435416 3.2.1.4 cotton + H2O - Gloeophyllum trabeum ? - ? 372763 3.2.1.4 cotton + H2O - Thermothelomyces thermophilus ? - ? 372763 3.2.1.4 cotton straw + H2O - Gracilibacillus sp. ? - ? 435417 3.2.1.4 crystalline cellulase + H2O - Pyrococcus horikoshii ? - ? 391698 3.2.1.4 crystalline cellulose - Pyrococcus horikoshii ? - ? 381699 3.2.1.4 crystalline cellulose + H2O - Pyrococcus horikoshii ? - ? 391699 3.2.1.4 crystalline cellulose + H2O as natural substrate for CEL9C1 Solanum lycopersicum ? - ? 391699 3.2.1.4 curdlan + H2O activity is 26% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ? - ? 94323 3.2.1.4 debranched arabinan activity is 25% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ? - ? 423292 3.2.1.4 dextran + H2O - Talaromyces funiculosus ? - ? 94189 3.2.1.4 filter paper + H2O - Thermothelomyces thermophilus cellobiose + ? - ? 373199 3.2.1.4 filter paper + H2O - Bacillus pumilus ? - ? 391981 3.2.1.4 filter paper + H2O - Humicola insolens ? - ? 391981 3.2.1.4 filter paper + H2O - Trichoderma reesei ? - ? 391981 3.2.1.4 filter paper + H2O - Aspergillus versicolor ? - ? 391981 3.2.1.4 filter paper + H2O - Thermochaetoides thermophila ? - ? 391981 3.2.1.4 filter paper + H2O - Mycothermus thermophilus ? - ? 391981 3.2.1.4 filter paper + H2O - Sporotrichum pulverulentum ? - ? 391981 3.2.1.4 filter paper + H2O - Aspergillus wentii ? - ? 391981 3.2.1.4 filter paper + H2O - Thermothelomyces thermophilus ? - ? 391981 3.2.1.4 filter paper + H2O - Paenibacillus barcinonensis ? - ? 391981 3.2.1.4 filter paper + H2O - Neurospora crassa ? - ? 391981 3.2.1.4 filter paper + H2O - Nilaparvata lugens ? - ? 391981 3.2.1.4 filter paper + H2O - Aspergillus niger ? 18% of the activity with carboxymethyl cellulose ? 391981 3.2.1.4 filter paper + H2O 8.4% of the activity with carboxymethyl cellulose Bacillus subtilis ? - ? 391981 3.2.1.4 filter paper + H2O 7.6% of the activity with carboxymethyl cellulose bacterium enrichment culture ? - ? 391981 3.2.1.4 filter paper + H2O 7.6% of the activtiy with carboxymethyl cellulose bacterium enrichment culture ? - ? 391981 3.2.1.4 filter paper + H2O 4% of the activity with carboxymethyl-cellulose. CBH6A and EgGH45, synergic effect, showing the highest efficiency in the ratio at 80:20 Phialophora sp. CGMCC 3328 ? - ? 391981 3.2.1.4 filter paper + H2O 8% of the activity with carboxymethyl-cellulose. CBH6A and EgGH45, synergic effect, showing the highest efficiency in the ratio at 80:20 Phialophora sp. CGMCC 3328 ? - ? 391981 3.2.1.4 filter paper + H2O best substrate of isoforms cellulase II and III Thermoascus aurantiacus ? - ? 391981 3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis ? - ? 391981 3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis NCIMB 9375 ? - ? 391981 3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis Gibson 46 ? - ? 391981 3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis JCM 2505 ? - ? 391981 3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis NRRL NRS-1264 ? - ? 391981 3.2.1.4 filter paper + H2O - Thermochaetoides thermophila CT2 ? - ? 391981 3.2.1.4 filter paper + H2O - Paenibacillus barcinonensis MG7 ? - ? 391981 3.2.1.4 filter paper + H2O 8.4% of the activity with carboxymethyl cellulose Bacillus subtilis LFS3 ? - ? 391981 3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis NBRC 12200 ? - ? 391981 3.2.1.4 filter paper + H2O - Bacillus pumilus S124A ? - ? 391981 3.2.1.4 filter paper + H2O - Trichoderma reesei QM6a ? - ? 391981 3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis ATCC 14580 ? - ? 391981 3.2.1.4 filter paper + H2O qualitative filter paper-grade 1 Bacillus licheniformis DSM 13 ? - ? 391981 3.2.1.4 filter paper + H2O - Trichoderma reesei NS 50013 ? - ? 391981 3.2.1.4 filter paper + H2O poor substrate Euphausia pacifica cellotriose + cellobiose + D-glucose major products ? 430185 3.2.1.4 glucan + H2O barley beta-glucan Pisum sativum ? - ? 93966 3.2.1.4 glucan + H2O barley beta-glucan Fibrobacter succinogenes ? - ? 93966 3.2.1.4 glucan + H2O yeast glucan Thermoascus aurantiacus ? - ? 93966 3.2.1.4 glucan + H2O catalytic domain of endoglucanase G Fibrobacter succinogenes ? - ? 93966 3.2.1.4 glucan + H2O barley beta-glucan Fibrobacter succinogenes S85 ? - ? 93966 3.2.1.4 glucan + H2O catalytic domain of endoglucanase G Fibrobacter succinogenes S85 ? - ? 93966 3.2.1.4 glucomannan + H2O - Phanerodontia chrysosporium ? - ? 115825 3.2.1.4 glucomannan + H2O - Bursaphelenchus xylophilus ? - ? 115825 3.2.1.4 glucomannan + H2O - Fomitopsis palustris ? - ? 115825 3.2.1.4 glucomannan + H2O - Fomitopsis palustris FFPRI 0507 ? - ? 115825 3.2.1.4 hydroxyethyl cellulose + H2O - Bacillus subtilis ? - ? 382048 3.2.1.4 hydroxyethyl cellulose + H2O - Komagataeibacter xylinus ? - ? 382048 3.2.1.4 hydroxyethyl cellulose + H2O - uncultured bacterium ? - ? 382048 3.2.1.4 hydroxyethyl cellulose + H2O - Bacillus subtilis AS3 ? - ? 382048 3.2.1.4 hydroxyethylcellulose + H2O - Trichoderma viride ? - ? 259797 3.2.1.4 hydroxyethylcellulose + H2O - Trichoderma reesei ? - ? 259797 3.2.1.4 hydroxyethylcellulose + H2O - Leishmania major ? - ? 259797 3.2.1.4 hydroxyethylcellulose + H2O - Trichoderma koningii ? - ? 259797 3.2.1.4 hydroxyethylcellulose + H2O - Melanocarpus albomyces ? - ? 259797 3.2.1.4 hydroxyethylcellulose + H2O - uncultured bacterium ? - ? 259797 3.2.1.4 hydroxyethylcellulose + H2O - Melanocarpus albomyces ALKO4237 ? - ? 259797 3.2.1.4 insoluble cellulose + H2O e.g. Avicel, Solka-floc, Sigmacell 50 Mucor circinelloides ? no glucose, cellobiose and short chain celloolifgosaccharides are formed ? 382064 3.2.1.4 insoluble cellulose + H2O e.g. Avicel, Solka-floc, Sigmacell 50 Mucor circinelloides NRRL 26519 ? no glucose, cellobiose and short chain celloolifgosaccharides are formed ? 382064 3.2.1.4 insoluble H3PO4 acid-swollen cellulose + H2O - Volvariella volvacea ? - ? 392263 3.2.1.4 konac glucomannan + H2O - Bacillus sp. (in: Bacteria) ? - ? 430279 3.2.1.4 konac glucomannan + H2O - Bacillus sp. (in: Bacteria) BG-CS10 ? - ? 430279 3.2.1.4 konjac glucomannan + H2O - Paenibacillus sp. M33 ? - ? 417127 3.2.1.4 konjac glucomannan + H2O substrate concentration 1% uncultured bacterium cellobiose + cellotriose - ? 447648 3.2.1.4 konjac mannan + H2O - Aspergillus terreus ? - ? 443667 3.2.1.4 konjac mannan + H2O - Aspergillus terreus NIH 2624 ? - ? 443667 3.2.1.4 laminarin + H2O - Bacillus subtilis ? - ? 94671 3.2.1.4 laminarin + H2O - Talaromyces funiculosus ? - ? 94671 3.2.1.4 laminarin + H2O - Thermotoga petrophila ? - ? 94671 3.2.1.4 laminarin + H2O - Rasamsonia emersonii ? - ? 94671 3.2.1.4 laminarin + H2O low activity uncultured bacterium ? - ? 94671 3.2.1.4 laminarin + H2O cellulase I Thermoascus aurantiacus ? - ? 94671 3.2.1.4 laminarin + H2O 5% of the activity with carboxymethyl cellulose Ganoderma lucidum ? - ? 94671 3.2.1.4 laminarin + H2O 9.1% of the activity with barley beta-glucan Aspergillus fischeri ? - ? 94671 3.2.1.4 laminarin + H2O - Thermotoga petrophila DSM 13995 ? - ? 94671 3.2.1.4 laminarin + H2O 9.1% of the activity with barley beta-glucan Aspergillus fischeri P1 ? - ? 94671 3.2.1.4 laminarin + H2O - Bacillus subtilis AS3 ? - ? 94671 3.2.1.4 laminarin + H2O - uncultured bacterium 6 D-glucose - ? 435584 3.2.1.4 lichenan + H2O - Pisum sativum ? - ? 94498 3.2.1.4 lichenan + H2O - Populus x canadensis ? - ? 94498 3.2.1.4 lichenan + H2O - Niallia circulans ? - ? 94498 3.2.1.4 lichenan + H2O - Fibrobacter succinogenes ? - ? 94498 3.2.1.4 lichenan + H2O - Talaromyces funiculosus ? - ? 94498 3.2.1.4 lichenan + H2O - Thermochaetoides thermophila ? - ? 94498 3.2.1.4 lichenan + H2O - Acetivibrio thermocellus ? - ? 94498 3.2.1.4 lichenan + H2O - Volvariella volvacea ? - ? 94498 3.2.1.4 lichenan + H2O - Gloeophyllum trabeum ? - ? 94498 3.2.1.4 lichenan + H2O - [Eubacterium] cellulosolvens ? - ? 94498 3.2.1.4 lichenan + H2O - Clostridium cellulovorans ? - ? 94498 3.2.1.4 lichenan + H2O - Bursaphelenchus xylophilus ? - ? 94498 3.2.1.4 lichenan + H2O - Saccharolobus solfataricus ? - ? 94498 3.2.1.4 lichenan + H2O - Bispora sp. MEY-1 ? - ? 94498 3.2.1.4 lichenan + H2O - Pyrococcus horikoshii ? - ? 94498 3.2.1.4 lichenan + H2O - Thermothelomyces thermophilus ? - ? 94498 3.2.1.4 lichenan + H2O - Rasamsonia emersonii ? - ? 94498 3.2.1.4 lichenan + H2O - uncultured bacterium ? - ? 94498 3.2.1.4 lichenan + H2O - Neurospora crassa ? - ? 94498 3.2.1.4 lichenan + H2O - Talaromyces verruculosus ? - ? 94498 3.2.1.4 lichenan + H2O - Paenibacillus sp. M33 ? - ? 94498 3.2.1.4 lichenan + H2O - Cryptopygus antarcticus ? - ? 94498 3.2.1.4 lichenan + H2O - Paenibacillus sp. Y2 ? - ? 94498 3.2.1.4 lichenan + H2O - Fomitopsis palustris ? - ? 94498 3.2.1.4 lichenan + H2O weak activity Ruminiclostridium cellulolyticum ? - ? 94498 3.2.1.4 lichenan + H2O best substrate Saccharolobus shibatae ? - ? 94498 3.2.1.4 lichenan + H2O high activity uncultured archaeon ? - ? 94498 3.2.1.4 lichenan + H2O 6% of the activity with carboxymethylcellulose Acetivibrio thermocellus ? - ? 94498 3.2.1.4 lichenan + H2O enzyme form EGD Acetivibrio thermocellus ? - ? 94498 3.2.1.4 lichenan + H2O 30% of the activity with barley beta-glucan, catalytic domain of endoglucanase G Fibrobacter succinogenes ? - ? 94498 3.2.1.4 lichenan + H2O endoglucanase A Cellulomonas fimi ? - ? 94498 3.2.1.4 lichenan + H2O cellulase I, II and III Thermoascus aurantiacus ? - ? 94498 3.2.1.4 lichenan + H2O 9% of the activity with barley beta-glucan Orpinomyces joyonii ? - ? 94498 3.2.1.4 lichenan + H2O activity of truncated enzyme form Ruminococcus albus ? - ? 94498 3.2.1.4 lichenan + H2O the enzyme hydrolyzes mixed linked beta-(1-3)(1-4) substrates such as barley glucan and lichenan more strongly than carboxymethylcellulose Thermotoga maritima ? - ? 94498 3.2.1.4 lichenan + H2O the smallest product is a disaccharide Acetivibrio thermocellus ? - ? 94498 3.2.1.4 lichenan + H2O 380% of the activity with carboxymethyl cellulose Salipaludibacillus agaradhaerens ? - ? 94498 3.2.1.4 lichenan + H2O activity is 120% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ? - ? 94498 3.2.1.4 lichenan + H2O the highest specific activity toward polysaccharides occurs with mixed-linkage (1->3),(1->4)-beta-D-glucans such as barley beta-D-glucan and lichenan Pyrococcus furiosus ? - ? 94498 3.2.1.4 lichenan + H2O and beta-glucan, preferred substrates Bacillus subtilis ? - ? 94498 3.2.1.4 lichenan + H2O 130% of the activity with carboxymethyl cellulose Saccharolobus solfataricus ? - ? 94498 3.2.1.4 lichenan + H2O 53.2% of the activity with barley beta-glucan Aspergillus fischeri ? - ? 94498 3.2.1.4 lichenan + H2O 60.5% of the activity with barley beta-glucan Humicola insolens ? - ? 94498 3.2.1.4 lichenan + H2O 77% of the activity with carboxymethyl cellulose Ganoderma lucidum ? - ? 94498 3.2.1.4 lichenan + H2O 94% of the activity on carboxymethyl cellulose Alicyclobacillus vulcanalis ? - ? 94498 3.2.1.4 lichenan + H2O 157% of the activity with carboxymethylcellulose Paenibacillus sp. X4 ? - ? 94498 3.2.1.4 lichenan + H2O activity of truncated enzyme form Ruminococcus albus F-40 ? - ? 94498 3.2.1.4 lichenan + H2O - Niallia circulans KSM-N257 ? - ? 94498 3.2.1.4 lichenan + H2O - Saccharolobus solfataricus P2 ? - ? 94498 3.2.1.4 lichenan + H2O 130% of the activity with carboxymethyl cellulose Saccharolobus solfataricus P2 ? - ? 94498 3.2.1.4 lichenan + H2O 53.2% of the activity with barley beta-glucan Aspergillus fischeri P1 ? - ? 94498 3.2.1.4 lichenan + H2O - Pyrococcus horikoshii DSM 12428 ? - ? 94498 3.2.1.4 lichenan + H2O and beta-glucan, preferred substrates Bacillus subtilis AS3 ? - ? 94498 3.2.1.4 lichenan + H2O 60.5% of the activity with barley beta-glucan Humicola insolens Y1 ? - ? 94498 3.2.1.4 lichenan + H2O 380% of the activity with carboxymethyl cellulose Salipaludibacillus agaradhaerens JAM-KU023 ? - ? 94498 3.2.1.4 lichenan + H2O best substrate Saccharolobus shibatae B12 ? - ? 94498 3.2.1.4 lichenan + H2O best substrate Saccharolobus shibatae DSM 5389 ? - ? 94498 3.2.1.4 lichenan + H2O - Acetivibrio thermocellus CelJ ? - ? 94498 3.2.1.4 lichenan + H2O - Neurospora crassa DSM 1257 ? - ? 94498 3.2.1.4 lichenan + H2O - Fibrobacter succinogenes S85 ? - ? 94498 3.2.1.4 lichenan + H2O 30% of the activity with barley beta-glucan, catalytic domain of endoglucanase G Fibrobacter succinogenes S85 ? - ? 94498 3.2.1.4 lichenan + H2O - Fomitopsis palustris FFPRI 0507 ? - ? 94498 3.2.1.4 lichenan + H2O enzyme hydrolyzes internal beta-1,4 glycosidic bonds within lichenan as well as carboxymethyl cellulose to release oligosaccharides and glucose Euphausia pacifica cellotriose + cellobiose + D-glucose major products ? 430355 3.2.1.4 lichenin + H2O - Cellulomonas sp. ? - ? 259800 3.2.1.4 lichenin + H2O best substrate uncultured bacterium ? - ? 259800 3.2.1.4 lignocellulose + H2O - Trichoderma reesei ? - ? 401318 3.2.1.4 lignocellulose + H2O solubilizes lignocellulose Orpinomyces sp. PC-2 ? - ? 401318 3.2.1.4 locust bean gum + H2O - uncultured archaeon ? - ? 373716 3.2.1.4 mannan + H2O - Volvariella volvacea ? - ? 369807 3.2.1.4 methyl cellulose + H2O - Fusarium oxysporum D-glucose - ? 435623 3.2.1.4 methylcellulose + H2O - Rasamsonia emersonii ? - ? 259801 3.2.1.4 methylcellulose + H2O - Rasamsonia emersonii CBS 814.70 ? - ? 259801 3.2.1.4 microcrystaliine cellulose + H2O - Thermochaetoides thermophila cellobiose + ? - ? 441639 3.2.1.4 microcrystalline cellulose + H2O endoglucanase 35 Fomitopsis palustris ? - ? 392651 3.2.1.4 microcrystalline cellulose + H2O endoglucanase 47 Fomitopsis palustris ? - ? 392651 3.2.1.4 milled aspen wood + H2O - Talaromyces verruculosus ? - ? 443738 3.2.1.4 additional information - Talaromyces funiculosus ? - ? 89 3.2.1.4 additional information no hydrolysis of laminarin Fibrobacter succinogenes ? - ? 89 3.2.1.4 additional information no hydrolysis of laminarin Ruminiclostridium cellobioparum ? - ? 89 3.2.1.4 additional information hydrolysis of cotton Trichoderma viride ? - ? 89 3.2.1.4 additional information hydrolysis of cotton Thermothelomyces heterothallicus ? - ? 89 3.2.1.4 additional information hydrolysis of cotton Thermochaetoides thermophila ? - ? 89 3.2.1.4 additional information hydrolysis of cotton Trichoderma koningii ? - ? 89 3.2.1.4 additional information the low-molecular weight enzyme form is able to form free fibres from filter paper Trichoderma viride ? - ? 89 3.2.1.4 additional information no activity with p-nitrophenyl-beta-D-glucoside Aspergillus niger ? - ? 89 3.2.1.4 additional information no activity with p-nitrophenyl-beta-D-glucoside Acetivibrio thermocellus ? - ? 89 3.2.1.4 additional information trans-glucosidase activity also observed Bacillus sp. (in: Bacteria) ? - ? 89 3.2.1.4 additional information hydrolysis of phosphoric-acid swollen filter paper Rasamsonia emersonii ? - ? 89 3.2.1.4 additional information hydrolysis of filter paper Niallia circulans ? - ? 89 3.2.1.4 additional information hydrolysis of filter paper Aspergillus terreus ? - ? 89 3.2.1.4 additional information hydrolysis of filter paper Trichoderma viride ? - ? 89 3.2.1.4 additional information hydrolysis of filter paper Catharanthus roseus ? - ? 89 3.2.1.4 additional information hydrolysis of filter paper Fusarium verticillioides ? - ? 89 3.2.1.4 additional information hydrolysis of filter paper Thermoascus aurantiacus ? - ? 89 3.2.1.4 additional information hydrolysis of filter paper Thermothelomyces heterothallicus ? - ? 89 3.2.1.4 additional information hydrolysis of filter paper Rasamsonia emersonii ? - ? 89 3.2.1.4 additional information hydrolysis of filter paper Bacillus cellulyticus K-12 ? - ? 89 3.2.1.4 additional information beta-1,3-linkages, alpha-1,6-linkages and beta-1,6-linkages are also susceptible to hydrolysis Talaromyces funiculosus ? - ? 89 3.2.1.4 additional information no activity with cellobiose Aspergillus niger ? - ? 89 3.2.1.4 additional information no activity with cellobiose Pisum sativum ? - ? 89 3.2.1.4 additional information no activity with cellobiose Irpex lacteus ? - ? 89 3.2.1.4 additional information no activity with cellobiose Acetivibrio thermocellus ? - ? 89 3.2.1.4 additional information no activity with cellobiose Ruminiclostridium cellobioparum ? - ? 89 3.2.1.4 additional information no activity with cellobiose Ruminiclostridium josui ? - ? 89 3.2.1.4 additional information when the oligosaccharides are modified by reduction with sodium borotritide, the second linkage from the reducing end becomes significantly susceptible to the enzyme and is preferentially cleaved Dolabella auricularia ? - ? 89 3.2.1.4 additional information Arg78 participates in catalysis Thermobifida fusca ? - ? 89 3.2.1.4 additional information modular enzyme that contains a family 30 carbohydrate-binding modules, CBM, and a family 9 catalytic module at its N-terminal moiety. The CBM is extremely important not only because it mediates the binding of the enzyme to the substrate but also because it participates in the catalytic function of the enzyme or contributes to maintain the correct tertiary structure of the family 9 catalytic module for expressing enzyme activity Acetivibrio thermocellus ? - ? 89 3.2.1.4 additional information no activity with avicell, xylan, galactan, arabinan, mannan or laminarin Rhizopus arrhizus ? - ? 89 3.2.1.4 additional information no activity with crystalline cellulose or cellobiose Psacothea hilaris ? - ? 89 3.2.1.4 additional information no activity with laminarin, avicel, pullulan and pachyman Orpinomyces joyonii ? - ? 89 3.2.1.4 additional information no activity with p-nitrophenyl cellobiose Ruminiclostridium cellulolyticum ? - ? 89 3.2.1.4 additional information no activity with tamarind xyloglucan, barley (1-3)(1-4)-beta-D-glucan, wheat arabinoxylan or birchwood xylan Brassica napus ? - ? 89 3.2.1.4 additional information no cleavage of cellotetraose, cellotriose and p-nitrophenyl-cellobiose Ruminiclostridium cellulolyticum ? - ? 89 3.2.1.4 additional information no hydrolysis of cellobioside, cellotrioside, cellotetraoside, laminarin, curdlan, xylan, p-nitrophenyl beta-D-cellotrioside and p-nitrophenyl beta-D-cellotetraoside Niallia circulans ? - ? 89 3.2.1.4 additional information no hydrolysis of cellulose, cotton, oat splet xylan and birchwood xylan Volvariella volvacea ? - ? 89 3.2.1.4 additional information transglycosylation activity with smaller soluble cellooligosaccharides Thermotoga maritima ? - ? 89 3.2.1.4 additional information effect of carbon source on enzyme activity Penicillium brasilianum ? - ? 89 3.2.1.4 additional information possible role of the enzyme in the softening of pericarp tissue and in the liquefaction of locules that takes place during ripening. Cel1 EGase responds to pathogen infection and supports a relationship between EGases, plant defense responses and fruit ripening. mRNA abundance is down-regulated in response to fungal infection. It is rapidly reduced 1 day after pathagen infection and completely suppressed 4 days after infection Solanum lycopersicum ? - ? 89 3.2.1.4 additional information endo-beta-1,4-glucanase EG27 shows no activity with p-nitrophenyl-beta-D-cellobiose, salicin and starch Ampullaria crossean ? - ? 89 3.2.1.4 additional information endo-beta-1,4-glucanase EG45 shows no activity with p-nitrophenyl-beta-D-cellobiose, salicin and starch Ampullaria crossean ? - ? 89 3.2.1.4 additional information no degradation of oat spelt xylan and larch wood xylan Mucor circinelloides ? - ? 89 3.2.1.4 additional information the enzyme consists of an N-terminal signal peptide, two glycosyl hydrolase family 5 catalytic modules, two novel carbohydrate-binding modules, two linker sequences, and a C-terminal sequence with an unknown function. Removal of the carbohydrate-binding modules from rCel5A reduces the catalytic activities with various polysaccharides remarkably [Eubacterium] cellulosolvens ? - ? 89 3.2.1.4 additional information Cel9A does not cleave xylan Cytophaga hutchinsonii ? - ? 89 3.2.1.4 additional information cellulase preparations that perform best on hardwood also show superior performance on the softwood substrates Penicillium sp. ? - ? 89 3.2.1.4 additional information cellulase preparations that perform best on hardwood also show superior performance on the softwood substrates Trichoderma sp. ? - ? 89 3.2.1.4 additional information distribution of cellulase between the residual substrate and supernatant during the course of enzymatic hydrolysis of steam-exploded wheat straw Penicillium decumbens ? - ? 89 3.2.1.4 additional information enzymatic deinking experiments, the ink removal rate in samples treated with the catalytic module is only slightly higher (about 8%), than that of untreated controls, whereas that of the EG1-treated samples is 100% higher. Bio-stoning of denim with EG1-CM results in increases of 48% and 40% in weight loss and indigo dye removal, respectively compared with untreated controls. These increases are considerably lower than the corresponding values of 219% and 133% obtained when samples are treated with EG1 Volvariella volvacea ? - ? 89 3.2.1.4 additional information no activity against crystalline forms of cellulose such as filter-paper or avicel and the beta-1,3-linked glucan laminarin. No activity with xylan unidentified microorganism ? - ? 89 3.2.1.4 additional information no activity on Avicel or xylan Cellulomonas flavigena ? - ? 89 3.2.1.4 additional information no activity with p-nitrophenyl-beta-D-glucopyranoside Bacillus amyloliquefaciens ? - ? 89 3.2.1.4 additional information no hydrolysis of cellobiose uncultured microorganism ? - ? 89 3.2.1.4 additional information the enzyme also shows chitosanase activity. Colloidal chitosan, chitosan and glycol chitosan are hydrolyzed by Cel8A at 15-40% the activity of cellulose Lysobacter sp. ? - ? 89 3.2.1.4 additional information the enzyme does not degrade curdran, xylan, laminarin and pectin (0.5% each) at all Salipaludibacillus agaradhaerens ? - ? 89 3.2.1.4 additional information the hydrolase exhibited chitosanase activity and cellulase activity Trichoderma viride ? - ? 89 3.2.1.4 additional information Xf818 carries out transglycosylation. The enzyme is unable to hydrolyse alpha-cellulose and laminarin (a beta-1,3 linked glucan) Xylella fastidiosa ? - ? 89 3.2.1.4 additional information among the processings of medicinal and aromatic plants, distillation waste of Cymbopogon winterianus, and bioprocessings of Artemisia annua (an industrial pharmaceutically important plant and source of artemisnin, an antimalarial compound) are selected on the basis of their capability to support higher levels of production of total cellulases, whereas garden waste, primarily consisting of Cynodon dactylon, is considered as the control, representing other lignocellulosic waste. Marc of Artemisia annua, a waste produced in huge amounts after the processing of the Artemisia annua herb, is found to be the suitable substrate for this fungus for maximizing the production of all three constituents of cellulase Trichoderma harzianum ? - ? 89 3.2.1.4 additional information among the processings of medicinal and aromatic plants, distillation waste of Cymbopogon winterianus, and bioprocessings of Artemisia annua (an industrial pharmaceutically important plant and source of artemisnin, an antimalarial compound) are selected on the basis of their capability to support higher levels of production of total cellulases, whereas garden waste, primarily consisting of Cynodon dactylon, is considered as the control, representing other lignocellulosic waste. Marc of Artemisia annua, a waste produced in huge amounts after the processing of the Artemisia annua herb, is found to be the suitable substrate for this fungus for maximizing the production of all three constituents of cellulase Trichoderma reesei ? - ? 89 3.2.1.4 additional information among the processings of medicinal and aromatic plants, distillation waste of Cymbopogon winterianus, and bioprocessings of Artemisia annua (an industrial pharmaceutically important plant and source of artemisnin, an antimalarial compound) are selected on the basis of their capability to support higher levels of production of total cellulases, whereas garden waste, primarily consisting of Cynodon dactylon, is considered as the control, representing other lignocellulosic waste. Marc of Artemisia annua, a waste produced in huge amounts after the processing of the Artemisia annua herb, is found to be the suitable substrate for this fungus for maximizing the production of all three constituents of cellulase Trichoderma virens ? - ? 89 3.2.1.4 additional information among the processings of medicinal and aromatic plants, distillation waste of Cymbopogon winterianus, and bioprocessings of Artemisia annua (an industrial pharmaceutically important plant and source of artemisnin, an antimalarial compound) are selected on the basis of their capability to support higher levels of production of total cellulases, whereas garden waste, primarily consisting of Cynodon dactylon, is considered as the control, representing other lignocellulosic waste. Marc of Artemisia annua, a waste produced in huge amounts after the processing of the Artemisia annua herb, is found to be the suitable substrate for this fungus for maximizing the production of all three constituents of cellulase Trichoderma citrinoviride ? - ? 89 3.2.1.4 additional information among the processings of medicinal and aromatic plants, distillation waste of Cymbopogon winterianus, and bioprocessings of Artemisia annua (an industrial pharmaceutically important plant and source of artemisnin, an antimalarial compound) are selected on the basis of their capability to support higher levels of production of total cellulases, whereas garden waste, primarily consisting of Cynodon dactylon, is considered as the control, representing other lignocellulosic waste. Marc of Artemisia annua, a waste produced in huge amounts after the processing of the Artemisia annua herb, is found to be the suitable substrate for this fungus for maximizing the production of all three constituents of cellulase Trichoderma strictipile ? - ? 89 3.2.1.4 additional information beta-1,4-endoglucanases show activities of the cleavage of the cellulose backbone due to an endocleavage. Oligosaccharides of different lengths as well as cellobiose and glucose are formed Pseudomonas putida ? - ? 89 3.2.1.4 additional information enzyme is essential for growth and development of potato cyst nematodes Solanum lycopersicum ? - ? 89 3.2.1.4 additional information carboxymethyl cellulose sodium salt is a better substrate than insoluble cellulose substrate such as Avicel (0 U/mg), SIGMACELL cellulose (0 U/mg), and CM cellulose Cryptococcus sp. S-2 ? - ? 89 3.2.1.4 additional information Cel7B has greater activity than the endoglucanases Cel45A and Cel7A against crystalline cellulose, whereas in the case of amorphous substrate the order is reversed Melanocarpus albomyces ? - ? 89 3.2.1.4 additional information cellobiose and xylan are no substrates Caecomyces sp. CR4 ? - ? 89 3.2.1.4 additional information enzyme does not release reducing sugars from Avicel microcrystalline cellulose, cellobiose, polygalacturonate, locust bean gum, xylan, Rhizobium leguminosarum bv. trifolii acidic heteropolysaccharide EPS types I, II, or III, or Sinorhizobium meliloti succinoglycan exopolysaccharide. Enzyme has a high substrate specificity for noncrystalline cellulose Rhizobium leguminosarum bv. trifolii ? - ? 89 3.2.1.4 additional information free cellulase compared with cellulase immobilized onto Si wafers and amino-terminated surfaces, amount of glucose produced by free cellulase is about 20% higher than that obtained from immobilized cellulase Trichoderma reesei ? - ? 89 3.2.1.4 additional information hydrolyzing internal beta-1,4-glycosidic bonds and resulting in a smear of polymers with different lengths. The hydrolytic products of tCfEG are one unit sugar less than those produced by nCfEG Coptotermes formosanus ? - ? 89 3.2.1.4 additional information investigation of substrate specificity Bacillus sp. (in: Bacteria) ? - ? 89 3.2.1.4 additional information starch as a substrate shows a relative activity of 0% and xlycan shows a relative activity of 0.9% Bacillus sp. (in: Bacteria) ? - ? 89 3.2.1.4 additional information wild-type and mutant can not hydrolyze Avicel, laminarin and chitosan Bacillus subtilis ? - ? 89 3.2.1.4 additional information glucanase hydrolyses beta-1,4-glycosidic bonds Cherax destructor ? - ? 89 3.2.1.4 additional information glucanase hydrolyses mainly beta-1,4-glycosidic bonds but is also capable of significant hydrolysis of beta-1,3-glycosidic bonds Gecarcoidea natalis ? - ? 89 3.2.1.4 additional information Bgl7A can effectively hydrolyze beta-1,4 bonds and some beta-1,3 linkages in beta-glucan. Belonging to the group of non-specific endoglucanase, Bgl7A can hydrolyze not only beta-glucan and cellulose but also laminarin and oat spelt xylan Bispora sp. MEY-1 ? - ? 89 3.2.1.4 additional information the fungal enzyme binds specifically to cyst wall cellulose of Acanthamoeba ssp., which causes keratitis in human cornea, overview Trichoderma reesei ? - ? 89 3.2.1.4 additional information carboxymethyl cellulose and beta-glucan are the best substrates with relative activities of 100% and 83%, respectively, birch wood xylan and oat spelt xylan have moderate activities of 68% and 60%, respectively, which otherwise supports low level of cellulase activity, Avicel has the lowest activity of 12%, substrate specificity, overview Salinivibrio sp. NTU-05 ? - ? 89 3.2.1.4 additional information CcCel6C exhibits high cellobiohydrolase activity Coprinopsis cinerea ? - ? 89 3.2.1.4 additional information inverting glycoside hydrolases catalyze bond cleavage using a single-displacement mechanism involving the participation of two acidic amino acid residues positioned opposite each other across the active site cleft or tunnel Cellulomonas fimi ? - ? 89 3.2.1.4 additional information poor activity with laminarin and oat spelt xylan, no activity with Avicel Bispora sp. MEY-1 ? - ? 89 3.2.1.4 additional information substrates are acid swollen cellulose, lichenan, beta-glucan, carboxymethyl cellulose, galactomannans, oat spelt xylan, avicel, and steam exploded bagasse, no activity glucomannan and laminarin Acetivibrio thermocellus ? - ? 89 3.2.1.4 additional information Cel9A presents both exo- and endo-cellulase activities, reaction mode, existence of an active conformer prior to ligand binding, overview Thermobifida fusca ? - ? 89 3.2.1.4 additional information EBI-244 is active on a range of high molecular weight carbohydrate substrates containing beta-1,4-linked glucose, including carboxymethyl cellulose, Avicel, and filter paper, it is active toward 4-nitrophenyl-cellobioside but inactive toward 4-nitrophenyl-glucoside Desulfurococcaceae archaeon EBI-244 ? - ? 89 3.2.1.4 additional information the enzyme shows multisubstrate specificity, showing significantly higher activity with lichenan and beta-glucan and lower activity with laminarin, hydroxyethylcellulose, and steam exploded bagasse Bacillus subtilis ? - ? 89 3.2.1.4 additional information the organism possesses carboxymethyl cellulase and cellulase activities, overview Bacillus sp. (in: Bacteria) ? - ? 89 3.2.1.4 additional information the organism possesses carboxymethyl cellulase and cellulase activities, overview Geobacillus sp. ? - ? 89 3.2.1.4 additional information Rucel5B is active towards carboxymethyl cellulose, barley glucan, lichenan, pNPC, PASC, avicel and Whatman NO.1 filter paper, but not active towards laminarin, beta-1,6-glucan and birchwood xylan uncultured rumen bacterium ? - ? 89 3.2.1.4 additional information substrate-binding mode of cellulase 12A, overview. A network of interactions exists between the enzyme and its substrate. The sugar residues bound to the enzyme appear to be more ordered in the 22 and 21 subsites than in the 11, 12 and 23 subsites. In the E134C crystals the bound 21 sugar at the cleavage site consistently show the alpha-anomeric configuration, implicating an intermediate-like structure Thermotoga maritima ? - ? 89 3.2.1.4 additional information with substrate cellulose, the substrate position is fixed by the alignment of one cellobiose unit between the two aromatic amino acid residues at subsites +1 and +2. During the enzyme reaction, the glucose structure of cellulose substrates is distorted at subsite -1, and the beta-1,4-glucoside bond between glucose moieties is twisted between subsites -1 and +1. Subsite -2 specifically recognizes the glucose residue, but recognition by subsites +1 and +2 is loose during the enzyme reaction. This type of recognition is important for creation of the distorted boat form of the substrate at subsite -1 Pyrococcus horikoshii ? - ? 89 3.2.1.4 additional information EglA hydrolyzes shorter cellodextrins (DP <5) as well as the amorphous portions of polysaccharides which contain only beta-1,4 bonds such as carboxymethyl cellulose, microcrystalline cellulose, Whatman paper, and cotton linter. Kinetics studies with cellooliogsaccharides and p-nitrophenyl-cellooligosaccharides indicated that the enzyme had three glucose binding subsites (-I, -II, and -III) for the nonreducing end and two glucose binding subsites (+I and +II) for the reducing end from the scissile glycosidic linkage. No activity is detected on solely beta-1,3-linked oligosaccharides or polysaccharides Pyrococcus furiosus ? - ? 89 3.2.1.4 additional information no hydrolysis of p-nitrophenyl-beta-D-cellobioside and p-nitrophenyl-beta-D-cellotrioside, no exocellulase activity Saccharolobus solfataricus ? - ? 89 3.2.1.4 additional information the enzyme does not hydrolyse 4-nitrophenyl beta-D-cellobioside and 4-nitrophenyl beta-D-cellotrioside. No activity could be detected with crystalline cellulose at both acidic and neutral pH values Saccharolobus solfataricus ? - ? 89 3.2.1.4 additional information the enzyme has a beta-1,4 hydrolytic mode of action. It shows no significant activity on PCM3/pachyman, microcrystalline cellulose, or on oat xylan. Concentrated solutions (80–90% v/v) of ionic liquids such as 1-ethyl-3-methylimidazolium acetate and 1,3-dimethylimidazolium dimethylphosphate are able to dissolve crystalline cellulose like AvicelW and cell wall polysaccharides, making them more accessible to hydrolytic enzymes Saccharolobus solfataricus ? - ? 89 3.2.1.4 additional information enzyme displays both beta-glucosidase, EC 3.2.1.21, and cellulase activities Bacillus pumilus ? - ? 89 3.2.1.4 additional information enzyme hydrolyzes soluble cellulose substrates containing beta-1,4-linkages, such as carboxylmethyl cellulose and konjac glucomannan, but has no exoglucanase and beta-glucosidase activities Bacillus sp. (in: Bacteria) ? - ? 89 3.2.1.4 additional information no substrates: 4-nitrophenyl beta-D-glucopyranoside, cellulose Bacillus subtilis ? - ? 89 3.2.1.4 additional information no substrates: cellotriose, cellobiose Euphausia pacifica ? - ? 89 3.2.1.4 additional information amino acids E209 and E319 act as proton donor and nucleophile in substrate catalytic domain Phanerodontia chrysosporium ? - ? 89 3.2.1.4 additional information enzyme displays broad substrate specificity and exhibits high activity on substrates containing beta-1,4-glycosidic bonds and beta-1,3-glycosidic bonds such as barley beta-glucan, laminarin, lichenan, carboxymethyl cellulose, carob bean gum, and birchwood xylan Rasamsonia emersonii ? - ? 89 3.2.1.4 additional information enzyme displays highest specific activity against beta-1,4-glycosidic bonds of various linear glucan polysaccharides. Poor or no substrates: avicel, pachyman, laminarin, starch uncultured bacterium ? - ? 89 3.2.1.4 additional information enzyme displays weak activity on weak on locust bean galactomannan, avicel, and filter paper Aspergillus fischeri ? - ? 89 3.2.1.4 additional information enzyme is most active against lichenan and beta-glucans and lesser active toward filter paper, carboxymethyl cellulose, and phosphoric acid-swollen cellulose Neurospora crassa ? - ? 89 3.2.1.4 additional information enzyme is strictly specific for the beta-1,4-glucoside linkage exhibiting activity toward barley beta-glucan, lichenan, and carboxymethyl cellulose sodium salt, but not toward laminarin (1,3-beta-glucan), birchwood xylan, avicel, and not toward 4-nitrophenyl beta-D-glucoside or 4-nitrophenyl cellobioside. Cel6C cleaves the internal glycosidic linkages of cellooligosaccharides randomly, the predominant product of polysaccharide hydrolysis is cellobiose Humicola insolens ? - ? 89 3.2.1.4 additional information no substrae: cellobiose Alicyclobacillus vulcanalis ? - ? 89 3.2.1.4 additional information no substrate: 4-nitrophenyl cellobioside uncultured bacterium ? - ? 89 3.2.1.4 additional information no substrate: cellulose Aspergillus niger ? - ? 89 3.2.1.4 additional information no substrates: alpha-glucan, cellobiose and xylan Bacillus subtilis ? - ? 89 3.2.1.4 additional information no substrates: avicel, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-cellobioside Rasamsonia emersonii ? - ? 89 3.2.1.4 additional information no substrates: chitosan, amylose Ganoderma lucidum ? - ? 89 3.2.1.4 additional information no substrates: PCM3/pachyman, avicel, oat xylan Saccharolobus solfataricus ? - ? 89 3.2.1.4 additional information no substrates: xylan, avicel, alpha-cellulose, filter paper, laminarin, curdlan Thermosipho sp. LD-2008 ? - ? 89 3.2.1.4 additional information Umcel9y-1 is an efficient endoglucanase and also exhibits high activities of exoglucanase and transglycosylation. The transglycosylation products of Umcel9y-1 including sophorose, laminaribiose, and gentiobiose, and transglycosylation are detected under all activated conditions. The decreasing order of catalytic efficiency for polysaccharides, cellooligosaccharides, and aryl-beta-glycosides is p-nitrophenyl-D-cellobioside, barley glucan, cellopentaose, cellotetraose, cellotriose, hydroxyethylcellulose, cellohexaose, laminarin, and carboxymethylcellulose, respectively uncultured bacterium ? - ? 89 3.2.1.4 additional information very poor or no substrates: laminarin, xyloglucan, birchwood xylan, arabinan, arabinoxylan, 4-nitrophenyl-beta-D-glucoside. Amino acids E209 and E319 act as proton donor and nucleophile in the catalytic domain Phanerodontia chrysosporium ? - ? 89 3.2.1.4 additional information very poor substrates: laminarin, xyloglucan. No substrates: birchwood xylan, arabinan, arabinoxylan, 4-nitrophenyl-beta-D-glucopyranoside Phanerodontia chrysosporium ? - ? 89 3.2.1.4 additional information the enzyme does not hydrolyze cellobiose and cellotriose Thermochaetoides thermophila ? - ? 89 3.2.1.4 additional information no activity against birchwood xylan, 4-nitrophenyl cellobiose, 4-nitrophenyl beta-D-glucoside Phialophora sp. CGMCC 3328 ? - ? 89 3.2.1.4 additional information enzyme exhibits both endoglucanase and xylanase activites, reactions of EC 3.2.1.4 and 3.2.1.8, respectively Thermothielavioides terrestris ? - ? 89 3.2.1.4 additional information no substrate: 4-methylumbelliferyl-beta-D-lactoside Melanocarpus albomyces ? - ? 89 3.2.1.4 additional information no substrates: beta-1,4-mannan, beta-1,4-glycol, chitosan or chitin Thermoascus aurantiacus ? - ? 89 3.2.1.4 additional information no substrates: cellotriose, cellobiose, p-nitrophenyl beta-D-cellobioside and p-nitrophenyl beta-D-cellotrioside, xylan, laminarin and lichenan, crystalline cellulose Saccharolobus solfataricus ? - ? 89 3.2.1.4 additional information no substrates: laminarin, curdlan Talaromyces verruculosus ? - ? 89 3.2.1.4 additional information no substrates: starch, xylan, cellobiose, filter paper Mycothermus thermophilus ? - ? 89 3.2.1.4 additional information very poor substrates: laminarin, xyloglucan, no substrates: birchwood xylan, arabinan, arabinoxylan, mannan, 4-nitrophenyl beta-D-glucopyranoside Phanerodontia chrysosporium ? - ? 89 3.2.1.4 additional information enzyme shows high endo-beta-1,4-mannanase activity versus various mannans, but low endo-beta-1,4 glucanase activity towards carboxymethylcellulose, andd negligible activity towards glucomannans Dictyoglomus turgidum ? - ? 89 3.2.1.4 additional information low hydrolytic activities, toward birchwood xylan and laminarin Paenibacillus sp. X4 ? - ? 89 3.2.1.4 additional information no substrate: cellotriose, insoluble cellulose, avicel, birchwood xylan, oat grass xylan, laminarin, soluble chitin Bellamya chinensis laeta ? - ? 89 3.2.1.4 additional information no substrates: avicel, arabinoxylan and xyloglucan Phanerodontia chrysosporium ? - ? 89 3.2.1.4 additional information no substrates: cellobiose and cellotriose Cryptopygus antarcticus ? - ? 89 3.2.1.4 additional information poor activity against filter paper and avicel uncultured microorganism ? - ? 89 3.2.1.4 additional information no activity against starch and Avicel. The recombinant enzyme saccharifies pre-treated wheat straw and bagasse to 3.32% and 3.2%, respectively after 6 h incubation at 85°C Thermotoga petrophila ? - ? 89 3.2.1.4 additional information bioconversion of the pretreated Arundo donax lignocellulosic biomass by wild-type and mutant enzymes Streptomyces sp. G12 ? - ? 89 3.2.1.4 additional information final hydrolysed product produced from carboxymethyl cellulose by chimera 1 is glucose confirming both beta-1,4-endoglucanase and beta-1,4-glucosidase activities, while the products of CtGH5-F194A point mutant are cellobiose and cello-oligosaccharides. Product indentification by thin layer chromatography. Enzymatic hydrolysis of 1% w/v Sorghum stalk pretreated by 1% NaOH by mutant CtGH5-F194A with or without wild-type enzyme CtGH1, which exhibits beta-glucosidase activity. No activity of the wild-type enzyme and mutant F194A with 4-nitrophenyl beta-D-glucoside, which is a substrate of the beta-glucosidase activity of chimeric mutant CtGH1-CtGH5-F194A Acetivibrio thermocellus ? - ? 89 3.2.1.4 additional information he later dominance of the dimeric product suggests that the trimer is slowly converted uncultured bacterium ? - ? 89 3.2.1.4 additional information hydrolysis of rice and corn straws Aspergillus glaucus ? - ? 89 3.2.1.4 additional information no activity towards the insoluble substrates crystalline cellulose and Avicel, and against curdlan, laminarin, xyloglucan, and xylan uncultured archaeon ? - ? 89 3.2.1.4 additional information no activity with Avicel, cotton, laminarin, and cellobiose Rhodococcus sp. ? - ? 89 3.2.1.4 additional information no activity with starch uncultured bacterium ? - ? 89 3.2.1.4 additional information substrate specificity, overview. Enzyme MtGH45 is unable to hydrolyze C3 and C4 oligosaccharides, cellobiose and cellotriose are detected as the main products when the enzyme is incubated with cellopentaose, which is completely hydrolyzed and the incubation with cellohexaose generates cellobiose, cellotriose, and cellotetraose as main products Thermothelomyces thermophilus ? - ? 89 3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis ? - ? 89 3.2.1.4 additional information substrate specificity, overview. The GtGH45 is able to convert all tested oligosaccharides to glucose, however, small amounts of cellobiose, cellotriose, and cellotetraose are detected in the reaction products, indicating that the enzyme cannot convert efficiently small oligosaccharides probably due their mode of recognition in the enzyme catalytic cleft. The degradation of C5 and C6 by GtGH45 is almost complete with glucose being a main product Gloeophyllum trabeum ? - ? 89 3.2.1.4 additional information synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components Thermochaetoides thermophila ? - ? 89 3.2.1.4 additional information the bifunctional enzyme also shows endoxylanase activity (EC 3.2.1.8) hydrolyzing beta-1,4-D-xylan from beechwood. Substrate specificity, overview. No endoglucanase activity on (+)-arabinogalactan, D-galacto-D-mannan, amylose, chitin, and sucrose. The enzyme shows activity with pretreated wheat straw and filter paper. CTendo7 produces cellooligosaccharides and xylooligosaccharides from the continuous enzymatic saccharification of carboxymethyl cellulose-Na and xylan, respectively Thermochaetoides thermophila ? - ? 89 3.2.1.4 additional information the crude enzyme releases reducing sugars from acid-pretreated straw at 75-85°C Saccharolobus shibatae ? - ? 89 3.2.1.4 additional information the enzyme harbors a carbohydrate binding module CBM2 and a glycoside hydrolase family 6 domain Cellulomonas fimi ? - ? 89 3.2.1.4 additional information the enzyme has a unique catalytic efficiency on beta-1,4-glucans rather than mixed beta-1,3/1,4-glucans as compared to other GH45 endoglucanases. No activity on Avicel and 4-nitrophenyl beta-D-cellobioside Fomitopsis palustris ? - ? 89 3.2.1.4 additional information the enzyme is able to hydrolyse sugarcane bagasse, rice husk, and wheat bran, with the highest production of reducers/fermentable sugars within 24 h of saccharification for wheat bran (137.21 mg/g). Saccharification of agroindustrial residues, overview Amphobotrys ricini ? - ? 89 3.2.1.4 additional information the enzyme is active with beta-D-glucans containing (1->3)- and (1->4)-bonds (EC 3.2.1.73, licheninase) and with beta-D-glucans containing only (1->4)-bonds (EC 3.2.1.4, cellulase). It shows an exclusive endoacting mechanism. No activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-cellobioside, avicel, curdlan, laminarin, cellulose, carboxymethyl cellulose, and xylan uncultured bacterium ? - ? 89 3.2.1.4 additional information the enzyme shows high affinity for carboxymethyl cellulose, but is also active with other substrates like beta-1,4-linked polysaccharides, including xyloglucan, glucomannan, beta-glucan, lichenin, and galactomannan Fervidobacterium nodosum ? - ? 89 3.2.1.4 additional information the enzyme strongly binds to filter paper despite having no recognizable carbohydrate binding module. No activity with cellotriose, 2,4-dinitrophenyl beta-cellobioside and 6-chloro-4-methylumbelliferyl beta-cellobioside. No cleavage of the internal beta-1,3-linkage is detected Cellulomonas fimi ? - ? 89 3.2.1.4 additional information the enzyme is able to hydrolyse sugarcane bagasse, rice husk, and wheat bran, with the highest production of reducers/fermentable sugars within 24 h of saccharification for wheat bran (137.21 mg/g). Saccharification of agroindustrial residues, overview Amphobotrys ricini URM 5627 ? - ? 89 3.2.1.4 additional information the enzyme harbors a carbohydrate binding module CBM2 and a glycoside hydrolase family 6 domain Cellulomonas fimi NBRC 15513 ? - ? 89 3.2.1.4 additional information the enzyme strongly binds to filter paper despite having no recognizable carbohydrate binding module. No activity with cellotriose, 2,4-dinitrophenyl beta-cellobioside and 6-chloro-4-methylumbelliferyl beta-cellobioside. No cleavage of the internal beta-1,3-linkage is detected Cellulomonas fimi NBRC 15513 ? - ? 89 3.2.1.4 additional information trans-glucosidase activity also observed Bacillus sp. (in: Bacteria) No. 1139 ? - ? 89 3.2.1.4 additional information no activity against starch and Avicel. The recombinant enzyme saccharifies pre-treated wheat straw and bagasse to 3.32% and 3.2%, respectively after 6 h incubation at 85°C Thermotoga petrophila DSM 13995 ? - ? 89 3.2.1.4 additional information no hydrolysis of cellobioside, cellotrioside, cellotetraoside, laminarin, curdlan, xylan, p-nitrophenyl beta-D-cellotrioside and p-nitrophenyl beta-D-cellotetraoside Niallia circulans KSM-N257 ? - ? 89 3.2.1.4 additional information synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components Thermochaetoides thermophila IMI 039719 ? - ? 89 3.2.1.4 additional information hydrolysis of cotton Thermothelomyces heterothallicus D-14 ? - ? 89 3.2.1.4 additional information hydrolysis of filter paper Thermothelomyces heterothallicus D-14 ? - ? 89 3.2.1.4 additional information the enzyme harbors a carbohydrate binding module CBM2 and a glycoside hydrolase family 6 domain Cellulomonas fimi NCIMB 8980 ? - ? 89 3.2.1.4 additional information the enzyme strongly binds to filter paper despite having no recognizable carbohydrate binding module. No activity with cellotriose, 2,4-dinitrophenyl beta-cellobioside and 6-chloro-4-methylumbelliferyl beta-cellobioside. No cleavage of the internal beta-1,3-linkage is detected Cellulomonas fimi NCIMB 8980 ? - ? 89 3.2.1.4 additional information the enzyme does not hydrolyse 4-nitrophenyl beta-D-cellobioside and 4-nitrophenyl beta-D-cellotrioside. No activity could be detected with crystalline cellulose at both acidic and neutral pH values Saccharolobus solfataricus P2 ? - ? 89 3.2.1.4 additional information the enzyme has a beta-1,4 hydrolytic mode of action. It shows no significant activity on PCM3/pachyman, microcrystalline cellulose, or on oat xylan. Concentrated solutions (80–90% v/v) of ionic liquids such as 1-ethyl-3-methylimidazolium acetate and 1,3-dimethylimidazolium dimethylphosphate are able to dissolve crystalline cellulose like AvicelW and cell wall polysaccharides, making them more accessible to hydrolytic enzymes Saccharolobus solfataricus P2 ? - ? 89 3.2.1.4 additional information no substrates: PCM3/pachyman, avicel, oat xylan Saccharolobus solfataricus P2 ? - ? 89 3.2.1.4 additional information enzyme displays weak activity on weak on locust bean galactomannan, avicel, and filter paper Aspergillus fischeri P1 ? - ? 89 3.2.1.4 additional information no degradation of oat spelt xylan and larch wood xylan Mucor circinelloides NRRL 26519 ? - ? 89 3.2.1.4 additional information no activity on Avicel or xylan Cellulomonas flavigena 531 ? - ? 89 3.2.1.4 additional information no substrate: cellotriose, insoluble cellulose, avicel, birchwood xylan, oat grass xylan, laminarin, soluble chitin Bellamya chinensis laeta UM-2014 ? - ? 89 3.2.1.4 additional information synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components Thermochaetoides thermophila DSM 1495 ? - ? 89 3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis NCIMB 9375 ? - ? 89 3.2.1.4 additional information no substrate: cellulose Aspergillus niger HO ? - ? 89 3.2.1.4 additional information the enzyme harbors a carbohydrate binding module CBM2 and a glycoside hydrolase family 6 domain Cellulomonas fimi NCTC 7547 ? - ? 89 3.2.1.4 additional information the enzyme strongly binds to filter paper despite having no recognizable carbohydrate binding module. No activity with cellotriose, 2,4-dinitrophenyl beta-cellobioside and 6-chloro-4-methylumbelliferyl beta-cellobioside. No cleavage of the internal beta-1,3-linkage is detected Cellulomonas fimi NCTC 7547 ? - ? 89 3.2.1.4 additional information enzyme does not release reducing sugars from Avicel microcrystalline cellulose, cellobiose, polygalacturonate, locust bean gum, xylan, Rhizobium leguminosarum bv. trifolii acidic heteropolysaccharide EPS types I, II, or III, or Sinorhizobium meliloti succinoglycan exopolysaccharide. Enzyme has a high substrate specificity for noncrystalline cellulose Rhizobium leguminosarum bv. trifolii ANU843 ? - ? 89 3.2.1.4 additional information effect of carbon source on enzyme activity Penicillium brasilianum IBT 20888 ? - ? 89 3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis Gibson 46 ? - ? 89 3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis JCM 2505 ? - ? 89 3.2.1.4 additional information the enzyme shows multisubstrate specificity, showing significantly higher activity with lichenan and beta-glucan and lower activity with laminarin, hydroxyethylcellulose, and steam exploded bagasse Bacillus subtilis AS3 ? - ? 89 3.2.1.4 additional information enzyme is strictly specific for the beta-1,4-glucoside linkage exhibiting activity toward barley beta-glucan, lichenan, and carboxymethyl cellulose sodium salt, but not toward laminarin (1,3-beta-glucan), birchwood xylan, avicel, and not toward 4-nitrophenyl beta-D-glucoside or 4-nitrophenyl cellobioside. Cel6C cleaves the internal glycosidic linkages of cellooligosaccharides randomly, the predominant product of polysaccharide hydrolysis is cellobiose Humicola insolens Y1 ? - ? 89 3.2.1.4 additional information the enzyme shows high affinity for carboxymethyl cellulose, but is also active with other substrates like beta-1,4-linked polysaccharides, including xyloglucan, glucomannan, beta-glucan, lichenin, and galactomannan Fervidobacterium nodosum ATCC 35602 ? - ? 89 3.2.1.4 additional information the enzyme does not degrade curdran, xylan, laminarin and pectin (0.5% each) at all Salipaludibacillus agaradhaerens JAM-KU023 ? - ? 89 3.2.1.4 additional information the crude enzyme releases reducing sugars from acid-pretreated straw at 75-85°C Saccharolobus shibatae B12 ? - ? 89 3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis NRRL NRS-1264 ? - ? 89 3.2.1.4 additional information the crude enzyme releases reducing sugars from acid-pretreated straw at 75-85°C Saccharolobus shibatae DSM 5389 ? - ? 89 3.2.1.4 additional information modular enzyme that contains a family 30 carbohydrate-binding modules, CBM, and a family 9 catalytic module at its N-terminal moiety. The CBM is extremely important not only because it mediates the binding of the enzyme to the substrate but also because it participates in the catalytic function of the enzyme or contributes to maintain the correct tertiary structure of the family 9 catalytic module for expressing enzyme activity Acetivibrio thermocellus CelJ ? - ? 89 3.2.1.4 additional information investigation of substrate specificity Bacillus sp. (in: Bacteria) CTP-09 ? - ? 89 3.2.1.4 additional information starch as a substrate shows a relative activity of 0% and xlycan shows a relative activity of 0.9% Bacillus sp. (in: Bacteria) CTP-09 ? - ? 89 3.2.1.4 additional information enzyme hydrolyzes soluble cellulose substrates containing beta-1,4-linkages, such as carboxylmethyl cellulose and konjac glucomannan, but has no exoglucanase and beta-glucosidase activities Bacillus sp. (in: Bacteria) BG-CS10 ? - ? 89 3.2.1.4 additional information enzyme shows high endo-beta-1,4-mannanase activity versus various mannans, but low endo-beta-1,4 glucanase activity towards carboxymethylcellulose, andd negligible activity towards glucomannans Dictyoglomus turgidum DSM 6724 ? - ? 89 3.2.1.4 additional information no substrate: 4-methylumbelliferyl-beta-D-lactoside Melanocarpus albomyces ALKO4237 ? - ? 89 3.2.1.4 additional information the enzyme harbors a carbohydrate binding module CBM2 and a glycoside hydrolase family 6 domain Cellulomonas fimi ATCC 484 ? - ? 89 3.2.1.4 additional information the enzyme strongly binds to filter paper despite having no recognizable carbohydrate binding module. No activity with cellotriose, 2,4-dinitrophenyl beta-cellobioside and 6-chloro-4-methylumbelliferyl beta-cellobioside. No cleavage of the internal beta-1,3-linkage is detected Cellulomonas fimi ATCC 484 ? - ? 89 3.2.1.4 additional information hydrolysis of filter paper Aspergillus terreus GN1 ? - ? 89 3.2.1.4 additional information enzyme is most active against lichenan and beta-glucans and lesser active toward filter paper, carboxymethyl cellulose, and phosphoric acid-swollen cellulose Neurospora crassa DSM 1257 ? - ? 89 3.2.1.4 additional information the enzyme harbors a carbohydrate binding module CBM2 and a glycoside hydrolase family 6 domain Cellulomonas fimi JCM 1341 ? - ? 89 3.2.1.4 additional information the enzyme strongly binds to filter paper despite having no recognizable carbohydrate binding module. No activity with cellotriose, 2,4-dinitrophenyl beta-cellobioside and 6-chloro-4-methylumbelliferyl beta-cellobioside. No cleavage of the internal beta-1,3-linkage is detected Cellulomonas fimi JCM 1341 ? - ? 89 3.2.1.4 additional information no hydrolysis of laminarin Fibrobacter succinogenes S85 ? - ? 89 3.2.1.4 additional information the enzyme also shows chitosanase activity. Colloidal chitosan, chitosan and glycol chitosan are hydrolyzed by Cel8A at 15-40% the activity of cellulose Lysobacter sp. IB-9374 ? - ? 89 3.2.1.4 additional information synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components Thermochaetoides thermophila CBS 144.50 ? - ? 89 3.2.1.4 additional information the enzyme has a unique catalytic efficiency on beta-1,4-glucans rather than mixed beta-1,3/1,4-glucans as compared to other GH45 endoglucanases. No activity on Avicel and 4-nitrophenyl beta-D-cellobioside Fomitopsis palustris FFPRI 0507 ? - ? 89 3.2.1.4 additional information no substrates: alpha-glucan, cellobiose and xylan Bacillus subtilis JS2004 ? - ? 89 3.2.1.4 additional information no substrates: 4-nitrophenyl beta-D-glucopyranoside, cellulose Bacillus subtilis LFS3 ? - ? 89 3.2.1.4 additional information hydrolysis of rice and corn straws Aspergillus glaucus CCHA ? - ? 89 3.2.1.4 additional information hydrolysis of filter paper Niallia circulans F-2 ? - ? 89 3.2.1.4 additional information the organism possesses carboxymethyl cellulase and cellulase activities, overview Bacillus sp. (in: Bacteria) DUSELR13 ? - ? 89 3.2.1.4 additional information wild-type and mutant can not hydrolyze Avicel, laminarin and chitosan Bacillus subtilis JA18 ? - ? 89 3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis NBRC 12200 ? - ? 89 3.2.1.4 additional information glucanase hydrolyses mainly beta-1,4-glycosidic bonds but is also capable of significant hydrolysis of beta-1,3-glycosidic bonds Gecarcoidea natalis Pocock 1888 ? - ? 89 3.2.1.4 additional information enzyme displays both beta-glucosidase, EC 3.2.1.21, and cellulase activities Bacillus pumilus S124A ? - ? 89 3.2.1.4 additional information no substrates: cellotriose, cellobiose, p-nitrophenyl beta-D-cellobioside and p-nitrophenyl beta-D-cellotrioside, xylan, laminarin and lichenan, crystalline cellulose Saccharolobus solfataricus DSM 1617 ? - ? 89 3.2.1.4 additional information the enzyme shows high affinity for carboxymethyl cellulose, but is also active with other substrates like beta-1,4-linked polysaccharides, including xyloglucan, glucomannan, beta-glucan, lichenin, and galactomannan Fervidobacterium nodosum DSM 5306 ? - ? 89 3.2.1.4 additional information the organism possesses carboxymethyl cellulase and cellulase activities, overview Geobacillus sp. WSUCFI ? - ? 89 3.2.1.4 additional information glucanase hydrolyses beta-1,4-glycosidic bonds Cherax destructor Clark 1936 ? - ? 89 3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis ATCC 14580 ? - ? 89 3.2.1.4 additional information the enzyme harbors a carbohydrate binding module CBM2 and a glycoside hydrolase family 6 domain Cellulomonas fimi DSM 20113 ? - ? 89 3.2.1.4 additional information the enzyme strongly binds to filter paper despite having no recognizable carbohydrate binding module. No activity with cellotriose, 2,4-dinitrophenyl beta-cellobioside and 6-chloro-4-methylumbelliferyl beta-cellobioside. No cleavage of the internal beta-1,3-linkage is detected Cellulomonas fimi DSM 20113 ? - ? 89 3.2.1.4 additional information no activity with p-nitrophenyl-beta-D-glucopyranoside Bacillus amyloliquefaciens DL-3 ? - ? 89 3.2.1.4 additional information hydrolysis of phosphoric-acid swollen filter paper Rasamsonia emersonii CBS 814.70 ? - ? 89 3.2.1.4 additional information hydrolysis of filter paper Rasamsonia emersonii CBS 814.70 ? - ? 89 3.2.1.4 additional information substrate specificity, overview. Poor activity with Avicel PH-101 Bacillus licheniformis DSM 13 ? - ? 89 3.2.1.4 additional information possible role of the enzyme in the softening of pericarp tissue and in the liquefaction of locules that takes place during ripening. Cel1 EGase responds to pathogen infection and supports a relationship between EGases, plant defense responses and fruit ripening. mRNA abundance is down-regulated in response to fungal infection. It is rapidly reduced 1 day after pathagen infection and completely suppressed 4 days after infection Solanum lycopersicum Mill. ? - ? 89 3.2.1.4 additional information distribution of cellulase between the residual substrate and supernatant during the course of enzymatic hydrolysis of steam-exploded wheat straw Penicillium decumbens JUA10 ? - ? 89 3.2.1.4 N,N',N'',N''', N''''-pentaacetylchitopentaose + H2O the enzyme liberates disaccharides from the reducing end Pyrococcus horikoshii N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose - ? 426707 3.2.1.4 N,N',N''-triacetyl-chitotriose + H2O the enzyme liberates disaccharides from the reducing end Pyrococcus horikoshii N,N'-diacetyl-chitobiose + N-acetyl-D-glucosamine - ? 426708 3.2.1.4 N-acetyllactosamine + butanol - Trichoderma reesei butyl beta-N-acetyllactosaminide + H2O - ? 373908 3.2.1.4 N-acetyllactosamine + ethanol - Trichoderma reesei ethyl beta-N-acetyllactosaminide + H2O - ? 373909 3.2.1.4 N-acetyllactosamine + glycerol - Trichoderma reesei glyceryl beta-N-acetyllactosaminide - ? 373910 3.2.1.4 N-acetyllactosamine + propanol - Trichoderma reesei propyl beta-N-acetyllactosaminide + H2O - ? 373911 3.2.1.4 N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate + H2O - Thermotoga maritima ? - ? 426759 3.2.1.4 N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate + H2O - Saccharolobus solfataricus ? - ? 426759 3.2.1.4 N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate + H2O - Saccharolobus solfataricus P2 ? - ? 426759 3.2.1.4 N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate + H2O - Saccharolobus solfataricus ? - ? 426760 3.2.1.4 N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate + H2O - Saccharolobus solfataricus ? + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid - ? 441689 3.2.1.4 noncrystalline cellulose + H2O as natural substrate for CEL7 Solanum lycopersicum ? - ? 398971 3.2.1.4 o-nitrophenyl beta-cellobioside + H2O - Clonostachys rosea ? - ? 382436 3.2.1.4 o-nitrophenyl beta-cellobioside + H2O - Trichoderma koningii ? - ? 382436 3.2.1.4 o-nitrophenyl beta-cellobioside + H2O - Trichocladium griseum ? - ? 382436 3.2.1.4 o-nitrophenyl beta-cellobioside + H2O - Fusarium solani-melongenae ? - ? 382436 3.2.1.4 o-nitrophenyl beta-cellobioside + H2O - Trichoderma reesei ? - ? 382436 3.2.1.4 o-nitrophenyl beta-cellobioside + H2O - Trichoderma citrinoviride ? - ? 382436 3.2.1.4 oat spelt xylan + H2O - [Eubacterium] cellulosolvens ? - ? 382438 3.2.1.4 oat spelt xylan + H2O - uncultured bacterium ? - ? 382438 3.2.1.4 oat spelt xylan + H2O - Thermoascus aurantiacus ? 92% saccharification within 36 h, preparation containing cellulase and xylanase ? 382438 3.2.1.4 oat spelt xylan + H2O activity is 25% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ? - ? 382438 3.2.1.4 oat spelt xylan + H2O hydrolysed at 76% compared to the hydrolysis of carboxymethylcellulose Saccharolobus solfataricus ? - ? 382438 3.2.1.4 oat spelt xylan + H2O 59% of the activity with carboxymethylcellulose uncultured microorganism ? - ? 382438 3.2.1.4 oat spelt xylan + H2O - Thermoascus aurantiacus RCKK ? 92% saccharification within 36 h, preparation containing cellulase and xylanase ? 382438 3.2.1.4 p-nitrophenyl beta-D-cellobioside + H2O - Niallia circulans glucose + cellobiose + ? - ? 259821 3.2.1.4 p-nitrophenyl beta-D-cellobioside + H2O - Irpex lacteus glucose + cellobiose + ? - ? 259821 3.2.1.4 p-nitrophenyl beta-D-cellobioside + H2O - Bacillus cellulyticus K-12 glucose + cellobiose + ? - ? 259821 3.2.1.4 p-nitrophenyl beta-D-cellobioside + H2O cellulase III preferentially attacks the aglycone linkage Trichoderma viride glucose + cellobiose + ? - ? 259821 3.2.1.4 p-nitrophenyl beta-D-cellobioside + H2O cellulase II-A preferentially attacks the holoside linkage of p-nitrophenyl beta-D-cellobioside, cellulase II-B attacks mainly the aglycone linkage. Synthesis of cellotriose from p-nitrophenyl beta-D-cellobioside by transfer of a glucosyl residue, possibly to cellobiose produced in the reaction mixture Trichoderma viride glucose + cellobiose + ? - ? 259821 3.2.1.4 p-nitrophenyl cellobiose + H2O - Pyrococcus horikoshii ? - ? 393093 3.2.1.4 p-nitrophenyl cellobiose + H2O - Pyrococcus horikoshii p-nitrophenol + cellobiose - ? 393094 3.2.1.4 p-nitrophenyl-beta-D-cellobioside + H2O - Dickeya chrysanthemi ? - ? 382490 3.2.1.4 p-nitrophenyl-beta-D-cellobioside + H2O - Pseudoalteromonas haloplanktis ? - ? 382490 3.2.1.4 p-nitrophenyl-beta-D-cellobioside + H2O endoglucanase EG25 Chrysosporium lucknowense ? - ? 382490 3.2.1.4 p-nitrophenyl-beta-D-cellobioside + H2O endoglucanase EG28 Chrysosporium lucknowense ? - ? 382490 3.2.1.4 p-nitrophenyl-beta-D-cellobioside + H2O endoglucanase EG44 Chrysosporium lucknowense ? - ? 382490 3.2.1.4 p-nitrophenyl-beta-D-cellobioside + H2O endoglucanase EG47 Chrysosporium lucknowense ? - ? 382490 3.2.1.4 p-nitrophenyl-beta-D-cellobioside + H2O endoglucanase EG51 Chrysosporium lucknowense ? - ? 382490 3.2.1.4 p-nitrophenyl-beta-D-cellobioside + H2O endoglucanase EG60 Chrysosporium lucknowense ? - ? 382490 3.2.1.4 p-nitrophenyl-beta-D-cellopentaoside + H2O - Thermotoga maritima ? - ? 374202 3.2.1.4 p-nitrophenyl-beta-D-cellotetraoside + H2O - Thermotoga maritima ? - ? 374203 3.2.1.4 p-nitrophenyl-cellobiose + H2O - Ruminiclostridium cellulolyticum ? - ? 374238 3.2.1.4 pachyman + H2O activity is 22% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ? - ? 94305 3.2.1.4 pectin + H2O - Thermochaetoides thermophila galacturonic acid oligosaccharides - ? 443836 3.2.1.4 phosphoric acid swollen cellulose + H2O - Trichoderma reesei ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O - Thermochaetoides thermophila ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O - Gloeophyllum trabeum ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O - Melanocarpus albomyces ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O - Thermothelomyces thermophilus ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O - Neurospora crassa ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O - Cellulomonas fimi ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Fomitopsis palustris ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O fluorimetric determination in cellulase activity assay using calcofluor white Trichoderma reesei ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O - Cellulomonas fimi NBRC 15513 ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O - Cellulomonas fimi NCIMB 8980 ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O - Cellulomonas fimi NCTC 7547 ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O - Thermochaetoides thermophila CT2 ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O - Cellulomonas fimi ATCC 484 ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O - Cellulomonas fimi JCM 1341 ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O - Cellulomonas fimi DSM 20113 ? - ? 374331 3.2.1.4 phosphoric acid swollen cellulose + H2O substrate concentration 1%, best substrate and high activity uncultured bacterium cellobiose + cellotriose - ? 447985 3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis D-glucose + cellobiose + cellotriose + cellotetraose - ? 447986 3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis NCIMB 9375 D-glucose + cellobiose + cellotriose + cellotetraose - ? 447986 3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis Gibson 46 D-glucose + cellobiose + cellotriose + cellotetraose - ? 447986 3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis JCM 2505 D-glucose + cellobiose + cellotriose + cellotetraose - ? 447986 3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis NRRL NRS-1264 D-glucose + cellobiose + cellotriose + cellotetraose - ? 447986 3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis NBRC 12200 D-glucose + cellobiose + cellotriose + cellotetraose - ? 447986 3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis ATCC 14580 D-glucose + cellobiose + cellotriose + cellotetraose - ? 447986 3.2.1.4 phosphoric acid swollen cellulose + H2O best substrate Bacillus licheniformis DSM 13 D-glucose + cellobiose + cellotriose + cellotetraose - ? 447986 3.2.1.4 phosphoric acid-swollen avicel + H2O - Thermochaetoides thermophila cellobiose + ? - ? 441872 3.2.1.4 phosphoric acid-swollen cellulose + H2O - Thermobifida fusca ? - ? 374332 3.2.1.4 phosphoric acid-swollen cellulose + H2O - Volvariella volvacea ? - ? 374332 3.2.1.4 phosphoric acid-swollen cellulose + H2O - Ruminiclostridium cellulolyticum ? - ? 374332 3.2.1.4 phosphoric acid-swollen cellulose + H2O - Lyrodus pedicellatus ? - ? 374332 3.2.1.4 phosphoric acid-swollen cellulose + H2O - Brassica napus ? - ? 374332 3.2.1.4 phosphoric acid-swollen cellulose + H2O - uncultured bacterium ? - ? 374332 3.2.1.4 phosphoric acid-swollen cellulose + H2O weak Ruminiclostridium cellulolyticum ? - ? 374332 3.2.1.4 phosphoric acid-swollen cellulose + H2O low activity Thermochaetoides thermophila ? - ? 374332 3.2.1.4 phosphoric acid-swollen cellulose + H2O - Thermobifida fusca Cel9A ? - ? 374332 3.2.1.4 phosphoric acid-swollen cellulose + H2O low activity Thermochaetoides thermophila CT2 ? - ? 374332 3.2.1.4 phosphoric acid-treated wheat straw + H2O - Thermoascus aurantiacus ? 74% saccharification within 36 h, preparation containing cellulase and xylanase ? 435733 3.2.1.4 phosphoric acid-treated wheat straw + H2O - Thermoascus aurantiacus RCKK ? 74% saccharification within 36 h, preparation containing cellulase and xylanase ? 435733 3.2.1.4 pretreated sorghum stover + H2O - Streptomyces sp. ? - ? 435744 3.2.1.4 pretreated sorghum stover + H2O - Streptomyces sp. DSK59 ? - ? 435744 3.2.1.4 pullulan + H2O - Talaromyces funiculosus ? - ? 94190 3.2.1.4 raffinose + H2O - Trichoderma atroviride ? - ? 94403 3.2.1.4 rice husk + H2O - Trichoderma reesei ? - ? 393424 3.2.1.4 rice husk + H2O - Trichoderma reesei NS 50013 ? - ? 393424 3.2.1.4 rice straw + H2O - Gracilibacillus sp. ? - ? 393426 3.2.1.4 rice straw + H2O - Gracilibacillus sp. SK1 ? - ? 393426 3.2.1.4 salicin + H2O low activity Thermothelomyces thermophilus ? - ? 13703 3.2.1.4 salicin + H2O low activity Thermothelomyces thermophilus D-14 ? - ? 13703 3.2.1.4 sea lettuce + H2O - Euphausia pacifica cellotriose + cellobiose + D-glucose major products ? 430708 3.2.1.4 Sigmacell 101 + H2O low activity, endo-beta-1,4-glucanase EG27 Ampullaria crossean ? - ? 382808 3.2.1.4 Sigmacell 101 + H2O low activity, endo-beta-1,4-glucanase EG45 Ampullaria crossean ? - ? 382808 3.2.1.4 sodium carboxymethyl cellulose + H2O - Bacillus subtilis ? - ? 401895 3.2.1.4 sodium carboxymethyl cellulose + H2O - Bacillus subtilis BME-15 ? - ? 401895 3.2.1.4 soluble cellodextrin + H2O - Mucor circinelloides ? no glucose, cellobiose and short chain cellooligosaccharides are formed ? 382819 3.2.1.4 soluble cellodextrin + H2O - Mucor circinelloides NRRL 26519 ? no glucose, cellobiose and short chain cellooligosaccharides are formed ? 382819 3.2.1.4 soluble cellulose + H2O endo mode of action. At the beginning only high molecular mass products are released suggesting an endowise action of the enzyme. The major product has a degree of polymerization of three, minor quantities of smaller and larger oligosaccharides are also produced. Not active against insoluble cellulose unidentified microorganism ? - ? 393515 3.2.1.4 soluble starch + H2O - Thermochaetoides thermophila ? - ? 94576 3.2.1.4 soybean husk + H2O - Trichoderma reesei ? - ? 430717 3.2.1.4 soybean husk + H2O - Trichoderma reesei NS 50013 ? - ? 430717 3.2.1.4 sugarcane bagasse + H2O - Trichoderma reesei ? - ? 374682 3.2.1.4 sugarcane bagasse + H2O - Bacillus sp. SR22 ? - ? 374682 3.2.1.4 sugarcane bagasse + H2O - Trichoderma reesei NS 50013 ? - ? 374682 3.2.1.4 sulfite-pulped spruce + H2O substrate concentration 1% uncultured bacterium cellobiose + cellotriose - ? 448149 3.2.1.4 tamarind xyloglucan + H2O - Cellulomonas fimi ? - ? 417690 3.2.1.4 tamarind xyloglucan + H2O - Cellulomonas fimi NBRC 15513 ? - ? 417690 3.2.1.4 tamarind xyloglucan + H2O - Cellulomonas fimi NCIMB 8980 ? - ? 417690 3.2.1.4 tamarind xyloglucan + H2O - Cellulomonas fimi NCTC 7547 ? - ? 417690 3.2.1.4 tamarind xyloglucan + H2O - Cellulomonas fimi ATCC 484 ? - ? 417690 3.2.1.4 tamarind xyloglucan + H2O - Cellulomonas fimi JCM 1341 ? - ? 417690 3.2.1.4 tamarind xyloglucan + H2O - Cellulomonas fimi DSM 20113 ? - ? 417690 3.2.1.4 Whatman filter paper + H2O - Thermotoga petrophila ? - ? 445974 3.2.1.4 Whatman filter paper + H2O - Thermotoga petrophila DSM 13995 ? - ? 445974 3.2.1.4 wheat straw + H2O - Trichoderma reesei ? - ? 393842 3.2.1.4 wheat straw + H2O - Gracilibacillus sp. ? - ? 393842 3.2.1.4 wheat straw + H2O - Gracilibacillus sp. SK1 ? - ? 393842 3.2.1.4 wheat straw + H2O - Trichoderma reesei NS 50013 ? - ? 393842 3.2.1.4 xylan + H2O - Saccharolobus shibatae ? - ? 94170 3.2.1.4 xylan + H2O - Aspergillus niger ? 20% of the activity with carboxymethyl cellulose ? 94170 3.2.1.4 xylan + H2O - Actinomyces sp. 40 ? 6.1% of the activity with carboxymethyl cellulose ? 94170 3.2.1.4 xylan + H2O weak activity Ruminiclostridium cellulolyticum ? - ? 94170 3.2.1.4 xylan + H2O about 2% of the activity with barley beta-glucan Orpinomyces joyonii ? - ? 94170 3.2.1.4 xylan + H2O activity of truncated enzyme form Ruminococcus albus ? - ? 94170 3.2.1.4 xylan + H2O the smallest product is a disaccharide Acetivibrio thermocellus ? - ? 94170 3.2.1.4 xylan + H2O low acrtivity, endo-beta-1,4-glucanase EG27 Ampullaria crossean ? - ? 94170 3.2.1.4 xylan + H2O low acrtivity, endo-beta-1,4-glucanase EG45 Ampullaria crossean ? - ? 94170 3.2.1.4 xylan + H2O 22.5% of the activity with carboxymethylcellulose Bacillus amyloliquefaciens ? - ? 94170 3.2.1.4 xylan + H2O 20.3% of the activity with carboxymethyl cellulose Bacillus subtilis ? - ? 94170 3.2.1.4 xylan + H2O 3.3% of the activity with carboxymethyl cellulose bacterium enrichment culture ? - ? 94170 3.2.1.4 xylan + H2O beechwood xylan, 34% , and birchwood xylan, 31% of the activity on carboxymethyl cellulose, respectively Alicyclobacillus vulcanalis ? - ? 94170 3.2.1.4 xylan + H2O activity of truncated enzyme form Ruminococcus albus F-40 ? - ? 94170 3.2.1.4 xylan + H2O - Aspergillus niger HO ? 20% of the activity with carboxymethyl cellulose ? 94170 3.2.1.4 xylan + H2O - Saccharolobus shibatae B12 ? - ? 94170 3.2.1.4 xylan + H2O - Saccharolobus shibatae DSM 5389 ? - ? 94170 3.2.1.4 xylan + H2O 20.3% of the activity with carboxymethyl cellulose Bacillus subtilis LFS3 ? - ? 94170 3.2.1.4 xylan + H2O - Actinomyces sp. 40 Korean Native Goat 40 ? 6.1% of the activity with carboxymethyl cellulose ? 94170 3.2.1.4 xylan + H2O 22.5% of the activity with carboxymethylcellulose Bacillus amyloliquefaciens DL-3 ? - ? 94170 3.2.1.4 xylan + H2O - Populus x canadensis additional information - ? 259815 3.2.1.4 xylan + H2O - Thermobifida fusca additional information - ? 259815 3.2.1.4 xylan + H2O - Talaromyces funiculosus additional information - ? 259815 3.2.1.4 xylan + H2O - Lenzites trabea additional information - ? 259815 3.2.1.4 xylan + H2O - Niallia circulans additional information main products: cellobiose + cellotetraose ? 259815 3.2.1.4 xylan + H2O no activity Acetivibrio thermocellus additional information - ? 259815 3.2.1.4 xylan + H2O endoglucanase B Cellulomonas fimi additional information - ? 259815 3.2.1.4 xylan + H2O hydrolysis with endoglucanase B, no action with endoglucanase A and C Athelia rolfsii additional information - ? 259815 3.2.1.4 xylan + H2O 6% of the activity with barley beta-glucan with the insoluble xylan from oats spelt, 27% of the activity with barley beta-glucan with the soluble xylan from oats spelt, catalytic domain of endoglucanase G Fibrobacter succinogenes additional information - ? 259815 3.2.1.4 xylan + H2O at 90°C, pH 4 by an enzyme mix consisting of SSO1354 and additional glycosyl hydrolases Saccharolobus solfataricus D-xylose + ? - ? 374918 3.2.1.4 xyloglucan + H2O - Populus x canadensis ? - ? 259816 3.2.1.4 xyloglucan + H2O - Populus alba ? - ? 259816 3.2.1.4 xyloglucan + H2O - Aspergillus niger ? 71% of the activity with carboxymethyl cellulose ? 259816 3.2.1.4 xyloglucan + H2O as natural substrate in CEL7 Solanum lycopersicum ? - ? 259816 3.2.1.4 xyloglucan + H2O isolated from tamarind Aspergillus terreus ? - ? 259816 3.2.1.4 xyloglucan + H2O a beta-1,4-1,6-glucan, 40% activity compared to barley beta-glucan uncultured bacterium ? - ? 259816 3.2.1.4 xyloglucan + H2O isolated from tamarind Aspergillus terreus NIH 2624 ? - ? 259816 3.2.1.4 xyloglucan + H2O - Aspergillus niger HO ? 71% of the activity with carboxymethyl cellulose ? 259816 3.2.1.4 yacon + H2O - Trichoderma reesei ? - ? 430778 3.2.1.4 yam + H2O - Trichoderma reesei ? - ? 430779 3.2.1.4 yeast glucan + H2O best substrate of isoform cellulase I, no substrate of isoforms cellulase II and III Thermoascus aurantiacus ? - ? 442084