3.2.1.31 (1S)-1-(chloromethyl)-3-([5-[2-(dimethylamino)ethoxy]-1H-indol-2-yl]carbonyl)-2,3-dihydro-1H-benzo[e]indol-5-yl beta-D-glucopyranosiduronic acid + H2O - Homo sapiens D-glucuronate + [(1S)-1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benzo[e]indol-3-yl][5-[2-(dimethylamino)ethoxy]-1H-indol-2-yl]methanone - ? 429456 3.2.1.31 (1S,3S)-3,12-dihydroxy-3-(hydroxyacetyl)-10-methoxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracen-1-yl 2,3,6-trideoxy-3-{[({2-[({[4-(beta-D-glucopyranuronosyloxy)-3-nitrobenzyl]oxy}carbonyl)amino]-5-({[(2-{[4-({[(pyridin-3-ylmethoxy)carbonyl]amino}methyl)benzoyl]amino}phenyl)carbamoyl]oxy}methyl)benzyl}oxy)carbonyl]amino}-alpha-D-lyxo-hexopyranoside + H2O - Homo sapiens D-glucuronate + (1S,3S)-3,12-dihydroxy-3-(hydroxyacetyl)-10-methoxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracen-1-yl 2,3,6-trideoxy-3-[({[2-({[(4-hydroxy-3-nitrobenzyl)oxy]carbonyl}amino)-5-({[(2-{[4-({[(pyridin-3-ylmethoxy)carbonyl]amino}methyl)benzoyl]amino}phenyl)carbamoyl]oxy}methyl)benzyl]oxy}carbonyl)amino]-alpha-D-lyxo-hexopyranoside - ? 429457 3.2.1.31 (4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)2 + H2O no activity with enzyme acting on chondroitin, 142% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ? - ? 371276 3.2.1.31 (4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)3 + H2O no activity with enzyme acting on chondroitin, 63% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ? - ? 371277 3.2.1.31 (4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)2 + H2O no activity with enzyme acting on chondroitin, 50% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ? - ? 371278 3.2.1.31 (4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)3 + H2O no activity with enzyme acting on chondroitin, 33% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ? - ? 371279 3.2.1.31 (4GlcAbeta1-3GalNAcbeta1-)2 + H2O 66% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 90% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ? - ? 371280 3.2.1.31 (4GlcAbeta1-3GalNAcbeta1-)3 + H2O 56% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 71% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ? - ? 371281 3.2.1.31 (4GlcAbeta1-3GlcNAcbeta1-)3 + H2O 81% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 73% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ? - ? 371282 3.2.1.31 1-deoxy-1-(6-thiopurinyl)-beta-D-glucopyranoside + H2O - Bos taurus 6-mercaptopurine + D-glucopyranose - ? 13970 3.2.1.31 1-deoxy-1-(6-thiopurinyl)-beta-D-glucopyranosiduronamide + H2O - Bos taurus 6-mercaptopurine + 1-deoxy-D-glucopyranosiduronamide - ? 13969 3.2.1.31 1-O-([4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl]carbamoyl)-beta-D-glucuronate + H2O - Homo sapiens D-glucuronate + [4-[(10R,11S)-10-[[((2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl]carbamic acid - ? 429598 3.2.1.31 1-O-[(2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamoyl]-beta-D-glucopyranuronic acid + H2O - Homo sapiens D-glucuronate + (2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamic acid - ? 429599 3.2.1.31 1-O-[(4-[[([[(1S,2R)-1-(benzoylamino)-3-[[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]-3-oxo-1-phenylpropan-2-yl]oxy]carbonyl)oxy]methyl]phenyl)carbamoyl]-beta-D-glucopyranuronic acid + H2O - Homo sapiens D-glucuronate + (4-[[([[(1S,2R)-1-(benzoylamino)-3-[[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]-3-oxo-1-phenylpropan-2-yl]oxy]carbonyl)oxy]methyl]phenyl)carbamic acid - ? 429600 3.2.1.31 2-amino-4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4,7-dimethyl-3,8,13-trioxo-11,13-diphenyl-2,9-dioxa-4,7,12-triazatridec-1-yl]phenyl beta-D-glucopyranosiduronate + H2O - Homo sapiens D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-1-(3-amino-4-hydroxyphenyl)-10-[(S)-(benzoylamino)(phenyl)methyl]-4,7-dimethyl-3,8-dioxo-2,9-dioxa-4,7-diazaundecan-11-oate - ? 429669 3.2.1.31 2-amino-4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl beta-D-glucopyranosiduronic acid + H2O - Homo sapiens D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-1-(3-amino-4-hydroxyphenyl)-10-[(S)-(benzoylamino)(phenyl)methyl]-4-methyl-3,8-dioxo-2,7,9-trioxa-4-azaundecan-11-oate - ? 429670 3.2.1.31 4-([[(2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamoyl]oxy]methyl)-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O - Homo sapiens D-glucuronate + 4-hydroxy-3-nitrobenzyl (2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamate - ? 429745 3.2.1.31 4-([[(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-9-yl]oxy]methyl)-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O - Homo sapiens D-glucuronate + (4S)-4-ethyl-4-hydroxy-9-[(4-hydroxy-3-nitrobenzyl)oxy]-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione - ? 429746 3.2.1.31 4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-beta-Gal-(1-3)-Gal + H2O rate of hydrolysis is 106% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger ? - ? 371964 3.2.1.31 4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal + H2O rate of hydrolysis is 117% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger ? - ? 371965 3.2.1.31 4-Me-beta-GlcA-(1-6)-Gal + H2O rate of hydrolysis is 62% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger ? - ? 371966 3.2.1.31 4-methyl-beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O - Neurospora crassa 4-methyl-D-glucuronate + beta-Gal(1-6)Gal 4.8% of the activity with 4-nitrophenyl beta-D-glucuronic acid ? 403881 3.2.1.31 4-methyl-beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O - Aspergillus niger 4-methyl-D-glucuronate + beta-Gal(1-6)Gal 42.6% of the activity with 4-nitrophenyl beta-D-glucuronic acid ? 403881 3.2.1.31 4-methylumbelliferyl beta-D-glucuronide + H2O - Homo sapiens 4-methylumbelliferone + D-glucuronic acid - ? 425691 3.2.1.31 4-methylumbelliferyl beta-D-glucuronide + H2O - Saccharolobus solfataricus 4-methylumbelliferone + D-glucuronic acid - ? 425691 3.2.1.31 4-methylumbelliferyl beta-D-glucuronide + H2O - Thermotoga maritima 4-methylumbelliferone + D-glucuronic acid - ? 425691 3.2.1.31 4-methylumbelliferyl beta-D-glucuronide + H2O - Saccharolobus solfataricus P2 4-methylumbelliferone + D-glucuronic acid - ? 425691 3.2.1.31 4-methylumbelliferyl beta-D-glucuronide + H2O - Littorina littorea 4-methylumbelliferone + beta-D-glucuronic acid - ? 446735 3.2.1.31 4-methylumbelliferyl beta-D-glucuronide + H2O - Mus musculus 4-methylumbelliferone + beta-D-glucuronic acid - ? 446735 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronic acid + H2O - Homo sapiens 4-methylumbelliferone + beta-D-glucuronic acid - ? 446749 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Drosophila melanogaster 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Escherichia coli 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Homo sapiens 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Zea mays 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Nicotiana tabacum 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Arabidopsis thaliana 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Bacillus sp. (in: Bacteria) 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Sphingomonas paucimobilis 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Lilium longiflorum 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Oryza sativa 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Lentinula edodes 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Aerococcus viridans 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Brassica juncea 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Staphylococcus warneri 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Lentinula edodes LD 106 4-methylumbelliferone + D-glucuronic acid - ? 13959 3.2.1.31 4-methylumbelliferyl-beta-D-glucuronide + H2O - Homo sapiens 4-methylumbelliferol + D-glucuronate - ? 411325 3.2.1.31 4-nitrophenol beta-D-glucuronide + H2O - Aspergillus oryzae 4-nitrophenol + beta-D-glucuronate - ? 446754 3.2.1.31 4-nitrophenol beta-D-glucuronide + H2O - Aspergillus oryzae Li-3 4-nitrophenol + beta-D-glucuronate - ? 446754 3.2.1.31 4-nitrophenyl beta-D-galactosiduronide + H2O 32% of the activity with 4-nitrophenyl beta-D-glucuronide Thermotoga maritima 4-nitrophenol + beta-D-galactosiduronic acid - ? 381182 3.2.1.31 4-nitrophenyl beta-D-galactosiduronide + H2O the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD Thermotoga maritima 4-nitrophenol + beta-D-galactosiduronic acid - ? 381182 3.2.1.31 4-nitrophenyl beta-D-galactosiduronide + H2O the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD Thermotoga maritima DSM 3109 4-nitrophenol + beta-D-galactosiduronic acid - ? 381182 3.2.1.31 4-nitrophenyl beta-D-galactosiduronide + H2O 32% of the activity with 4-nitrophenyl beta-D-glucuronide Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 4-nitrophenol + beta-D-galactosiduronic acid - ? 381182 3.2.1.31 4-nitrophenyl beta-D-glucopyranosiduronic acid + 4-nitrophenyl beta-D-glucopyranosiduronic acid - Bos taurus 4-nitrophenol + 4-nitrophenyl 3-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid + 4-nitrophenyl 2-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid - ? 374641 3.2.1.31 4-nitrophenyl beta-D-glucopyranosiduronic acid + 4-nitrophenyl beta-D-glucopyranosiduronic acid the enzyme shows transglucuronidation activity: when sodium (p-nitrophenyl beta-D-glucopyranoside)uronate is used as an acceptor and as a donor, sodium (sodium beta-D-glucopyranosyluronate)-(1-3)-(p-nitrophenyl beta-D-glucopyranosid)uronate and sodium (sodium beta-D-glucopyranosyluronate)-(1-2)-(p-nitrophenyl beta-D-glucopyranosid)uronate are obtained in yields of 18% and 15% respectively Bos taurus 4-nitrophenol + 4-nitrophenyl 3-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid + 4-nitrophenyl 2-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid - ? 374641 3.2.1.31 4-nitrophenyl beta-D-glucoside + H2O the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD Thermotoga maritima 4-nitrophenol + D-glucose - ? 13708 3.2.1.31 4-nitrophenyl beta-D-glucoside + H2O the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD Thermotoga maritima DSM 3109 4-nitrophenol + D-glucose - ? 13708 3.2.1.31 4-nitrophenyl beta-D-glucoside + H2O - Aspergillus niger 4-nitrophenol + beta-D-glucose - ? 364700 3.2.1.31 4-nitrophenyl beta-D-glucoside + H2O - Neurospora crassa 4-nitrophenol + beta-D-glucose - ? 364700 3.2.1.31 4-nitrophenyl beta-D-glucoside + H2O low activity Thermotoga maritima 4-nitrophenol + beta-D-glucose - ? 364700 3.2.1.31 4-nitrophenyl beta-D-glucoside + H2O low activity Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 4-nitrophenol + beta-D-glucose - ? 364700 3.2.1.31 4-nitrophenyl beta-D-glucuronic acid + H2O - Aspergillus niger 4-nitrophenol + D-glucuronic acid - ? 403939 3.2.1.31 4-nitrophenyl beta-D-glucuronic acid + H2O - Neurospora crassa 4-nitrophenol + D-glucuronic acid - ? 403939 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Escherichia coli 4-nitrophenol + D-glucuronic acid - ? 13958 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Homo sapiens 4-nitrophenol + D-glucuronic acid - ? 13958 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Rattus norvegicus 4-nitrophenol + D-glucuronic acid - ? 13958 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Bos taurus 4-nitrophenol + D-glucuronic acid - ? 13958 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Oryctolagus cuniculus 4-nitrophenol + D-glucuronic acid - ? 13958 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Nicotiana tabacum 4-nitrophenol + D-glucuronic acid - ? 13958 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Arabidopsis thaliana 4-nitrophenol + D-glucuronic acid - ? 13958 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Littorina littorea 4-nitrophenol + D-glucuronic acid - ? 13958 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Protubera nipponica 4-nitrophenol + D-glucuronic acid - ? 13958 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Lactobacillus gasseri 4-nitrophenol + D-glucuronic acid - ? 13958 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Streptococcus equi 4-nitrophenol + D-glucuronic acid - ? 13958 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD Thermotoga maritima 4-nitrophenol + D-glucuronic acid - ? 13958 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Lactobacillus gasseri ATCC 33323 4-nitrophenol + D-glucuronic acid - ? 13958 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD Thermotoga maritima DSM 3109 4-nitrophenol + D-glucuronic acid - ? 13958 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Mus musculus 4-nitrophenol + beta-D-glucuronic acid - ? 381184 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Escherichia coli 4-nitrophenol + beta-D-glucuronic acid - ? 381184 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - [Ruminococcus] gnavus 4-nitrophenol + beta-D-glucuronic acid - ? 381184 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O best substrate Thermotoga maritima 4-nitrophenol + beta-D-glucuronic acid - ? 381184 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - [Ruminococcus] gnavus E1 4-nitrophenol + beta-D-glucuronic acid - ? 381184 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O best substrate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 4-nitrophenol + beta-D-glucuronic acid - ? 381184 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Homo sapiens 4-nitrophenol + beta-D-glucuronate - ? 411369 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Bacteroides fragilis 4-nitrophenol + beta-D-glucuronate - ? 411369 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Levilactobacillus brevis 4-nitrophenol + beta-D-glucuronate - ? 411369 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Bacteroides ovatus 4-nitrophenol + beta-D-glucuronate - ? 411369 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Bacteroides uniformis 4-nitrophenol + beta-D-glucuronate - ? 411369 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Escherichia coli 4-nitrophenol + beta-D-glucuronate - ? 411369 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Clostridium perfringens 4-nitrophenol + beta-D-glucuronate - ? 411369 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Streptococcus agalactiae 4-nitrophenol + beta-D-glucuronate - ? 411369 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Lachnospira eligens 4-nitrophenol + beta-D-glucuronate - ? 411369 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Parabacteroides merdae 4-nitrophenol + beta-D-glucuronate - ? 411369 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Phocaeicola dorei 4-nitrophenol + beta-D-glucuronate - ? 411369 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Clostridium perfringens 13 4-nitrophenol + beta-D-glucuronate - ? 411369 3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O - Levilactobacillus brevis RO1 4-nitrophenol + beta-D-glucuronate - ? 411369 3.2.1.31 4-nitrophenyl beta-D-xylopyranoside + H2O preferred substrate Escherichia coli 4-nitrophenol + beta-D-xylopyranose - ? 115810 3.2.1.31 4-nitrophenyl D-glucuronide + H2O - Escherichia coli 4-nitrophenol + D-glucuronate - ? 411379 3.2.1.31 4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]-2-nitrophenyl beta-D-glucuronate + H2O - Homo sapiens D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-10-[(S)-(benzoylamino)(phenyl)methyl]-1-(4-hydroxy-3-nitrophenyl)-4-methyl-3,8-dioxo-2,7,9-trioxa-4-azaundecan-11-oate - ? 429804 3.2.1.31 4-[([[(3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)methyl]-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O - Homo sapiens D-glucuronate + 4-hydroxy-3-nitrobenzyl (3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-carboxylate - ? 429805 3.2.1.31 4-[([[(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-10-yl]carbamoyl]oxy)methyl]phenyl beta-D-glucopyranosiduronic acid + H2O - Homo sapiens D-glucuronate + 4-hydroxybenzyl [(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-10-yl]carbamate - ? 429806 3.2.1.31 4-[2-[1-(2-[2-[2-(beta-D-glucopyranuronosyloxy)ethoxy]ethoxy]ethyl)-1H-1,2,3-triazol-4-yl]-1-([[(3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)ethyl]-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O - Homo sapiens D-glucuronate + 2-[2-(2-[4-[2-([[(3beta,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)-2-(4-hydroxy-3-nitrophenyl)ethyl]-1H-1,2,3-triazol-1-yl]ethoxy)ethoxy]ethyl beta-D-glucopyranosiduronic acid - ? 429807 3.2.1.31 5-bromo-4-chloro-3-indoyl-beta-D-glucuronide + H2O - Escherichia coli 5-bromo-4-chloro-1H-indol-3-ol + beta-D-glucuronate - ? 411400 3.2.1.31 alpha-L-arabinosidase-treated-arabinogalactan-protein + H2O - Aspergillus niger ? - ? 372337 3.2.1.31 ammonium 1-deoxy-1-(6-thiopurinyl)-beta-D-glucopyranosidurate + H2O - Bos taurus 6-mercaptopurine + ammonium 1-deoxy-(6-thiopurinyl)-beta-D-glucopyranosiduronate - ? 13968 3.2.1.31 androstendione-enol-beta-D-glucuronide + H2O - Mammalia ? - ? 13965 3.2.1.31 apigenin 7,4'-diglucuronide + H2O - Secale cereale apigenin 7-O-glucuronide + glucuronic acid - ? 13964 3.2.1.31 baicalin + H2O - Levilactobacillus brevis baicalein + beta-D-glucuronate - ? 411701 3.2.1.31 baicalin + H2O a major flavonoid derived from the root of Scutellaria baicalensis Escherichia coli baicalein + beta-D-glucuronate productivity of 73% ? 411701 3.2.1.31 baicalin + H2O - Levilactobacillus brevis RO1 baicalein + beta-D-glucuronate - ? 411701 3.2.1.31 beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O - Neurospora crassa D-glucuronate + beta-Gal(1-6)Gal 67.4% of the activity with 4-nitrophenyl beta-D-glucuronic acid ? 404518 3.2.1.31 beta-GlcA(1-6)betaGal(1-6)Gal + H2O - Aspergillus niger D-glucuronate + beta-Gal(1-6)Gal 94.6% of the activity with 4-nitrophenyl beta-D-glucuronic acid ? 404519 3.2.1.31 beta-GlcA-(1-3)-Gal + H2O rate of hydrolysis is 2% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger ? - ? 372560 3.2.1.31 beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal + H2O rate of hydrolysis is 67% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger beta-Gal-(1-6)-Gal + beta-D-glucuronic acid - ? 372561 3.2.1.31 beta-GlcA-(1-6)-Gal + H2O rate of hydrolysis is 76% of the with p-nitrophenyl-beta-D-glucuronide Aspergillus niger D-galactose + beta-D-glucuronic acid - ? 372562 3.2.1.31 bilirubin diglucuronide + H2O - Escherichia coli bilirubin + D-glucuronate - ? 372572 3.2.1.31 bilirubin diglucuronide + H2O - Escherichia coli HGU-3 bilirubin + D-glucuronate - ? 372572 3.2.1.31 carboxyumbelliferyl-beta-D-glucuronide + H2O - Escherichia coli carboxyumbelliferone + beta-D-glucuronate - ? 416753 3.2.1.31 chondroitin + H2O MW 3500 Da: 23% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 41% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide. MW 8000 Da: 11% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 28% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide. MW 15000 Da: 6% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 9% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ? - ? 372707 3.2.1.31 curcumin beta-D-glucuronide + H2O - Mus musculus curcumin + beta-D-glucuronic acid - ? 447353 3.2.1.31 curcumin-beta-D-glucuronide + H2O curcumin, despite low systemic bioavailability, may be enzymatically activated (deconjugated) within GUSB-enriched bone to exert protective effects, a metabolic process that can also contribute to bone-protective effects of other highly glucuronidated dietary polyphenols Mus musculus curcumin + beta-D-glucuronic acid - ? 446216 3.2.1.31 daidzein 7-O-beta-D-glucuronide + H2O - Homo sapiens daidzein + D-glucuronate - ? 404946 3.2.1.31 dermatan sulfate + H2O - Mammalia ? - ? 115730 3.2.1.31 equol 7-O-beta-D-glucuronide + H2O - Homo sapiens equol + D-glucuronate - ? 405078 3.2.1.31 estriol 3-glucuronide + H2O - Escherichia coli estriol + D-glucuronic acid - ? 452770 3.2.1.31 GlcA-beta(1-6)Gal + H2O 49.9% of the activity with 4-nitrophenyl beta-D-glucuronic acid Neurospora crassa D-glucuronate + D-galactopyranose - ? 405252 3.2.1.31 GlcAbeta(1-6)Gal + H2O 85.7% of the activity with 4-nitrophenyl beta-D-glucuronic acid Aspergillus niger D-glucuronate + D-galactopyranose - ? 405254 3.2.1.31 GlcAbeta1-3GalNAc(4-OSO3-) + H2O no activity with enzyme acting on chondroitin, 4% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + N-acetylamino-beta-D-galactopyranose 4-sulfate - ? 372111 3.2.1.31 glucuronic acid 1-phosphate + H2O - Mammalia glucuronic acid + phosphate - ? 13966 3.2.1.31 glycyrrhetinic acid 3-O-mono-beta-D-glucuronide + H2O the enzyme shows similar activities toward both glycyrrhizin and glycyrrhetinic acid 3-O-mono-beta-D-glucuronide Aspergillus oryzae glycyrrhetic acid + D-glucuronate - ? 447576 3.2.1.31 glycyrrhetinic acid 3-O-mono-beta-D-glucuronide + H2O the enzyme shows similar activities toward both glycyrrhizin and glycyrrhetinic acid 3-O-mono-beta-D-glucuronide Aspergillus oryzae Li-3 glycyrrhetic acid + D-glucuronate - ? 447576 3.2.1.31 glycyrrhizin + H2O - Talaromyces purpureogenus 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid - ? 382007 3.2.1.31 glycyrrhizin + H2O specific substrate of isozymes I and II Streptococcus sp. 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid - ? 382007 3.2.1.31 glycyrrhizin + H2O specific substrate of isozymes I and II Streptococcus sp. LJ-22 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid - ? 382007 3.2.1.31 glycyrrhizin + H2O the enzyme shows similar activities toward both glycyrrhizin and glycyrrhetinic acid 3-O-mono-beta-D-glucuronide Aspergillus oryzae glycyrrhetinic acid 3-O-mono-beta-D-glucuronide + D-glucuronate - ? 447578 3.2.1.31 glycyrrhizin + H2O the enzyme shows similar activities toward both glycyrrhizin and glycyrrhetinic acid 3-O-mono-beta-D-glucuronide Aspergillus oryzae Li-3 glycyrrhetinic acid 3-O-mono-beta-D-glucuronide + D-glucuronate - ? 447578 3.2.1.31 heparan sulfate + H2O - Mammalia ? - ? 94461 3.2.1.31 kaempferol 3-O-beta-D-glucuronide + H2O - Homo sapiens kaempferol + D-glucuronate - ? 405519 3.2.1.31 luteolin 7-O-glucuronide + H2O - Secale cereale ? - ? 13963 3.2.1.31 luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] + H2O - Secale cereale ? - ? 13962 3.2.1.31 luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]-4'-O-glucuronide + H2O - Secale cereale ? - ? 13961 3.2.1.31 luteolin triglucuronide + H2O - Secale cereale ? - ? 369527 3.2.1.31 methylumbelliferyl-beta-D-glucuronide + H2O - Escherichia coli methylumbelliferone + beta-D-glucuronate - ? 417303 3.2.1.31 additional information - Littorina littorea ? - ? 89 3.2.1.31 additional information - Protubera nipponica ? - ? 89 3.2.1.31 additional information transfer of beta-glucuronosyl residues from aryl and alicyclic glucuronides to aliphatic alcohols and glycerols Mammalia ? - ? 89 3.2.1.31 additional information transglycosylation activity Aspergillus niger ? - ? 89 3.2.1.31 additional information role in physiology, in tissues, in body fluids Mammalia ? - ? 89 3.2.1.31 additional information role in physiology, in tissues, in body fluids Bacteria ? - ? 89 3.2.1.31 additional information role in physiology, in tissues, in body fluids Chondrichthyes ? - ? 89 3.2.1.31 additional information activity is inducible by Met-Gly Escherichia coli ? - ? 89 3.2.1.31 additional information activity is not inducible by Met-Gly Bacillus sp. (in: Bacteria) ? - ? 89 3.2.1.31 additional information activity is not inducible by Met-Gly Sphingomonas paucimobilis ? - ? 89 3.2.1.31 additional information activity is not inducible by Met-Gly Staphylococcus warneri ? - ? 89 3.2.1.31 additional information activity is slightly inducible by Met-Gly Aerococcus viridans ? - ? 89 3.2.1.31 additional information the potent beta-glucuronidase activity caused by the glucuronic acid conjugates from xenobiotics and endogenous compounds is a prime factor in the etiology of colon cancer Escherichia coli ? - ? 89 3.2.1.31 additional information when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed Escherichia coli ? - ? 89 3.2.1.31 additional information no activity with enzyme acting on chondroitin: N-acetylhyalobiuronic acid, N-acetylchondrosine, (4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)3, (4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)2, GlcAbeta1-3galNAc(4-OSO3-), (4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)3, (4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)2. No activity with enzyme acting on p-nitrophenyl-beta-D-glucuronide: N-acetylhyalobiuronic acid, N-acetylchondrosine Oryctolagus cuniculus ? - ? 89 3.2.1.31 additional information the enzyme is involved in glycosaminoglycan metabolism Pomacea sp. ? - ? 89 3.2.1.31 additional information exoglucuronidase Pomacea sp. ? - ? 89 3.2.1.31 additional information no activity of isozymes I and II with 4-nitrophenyl beta-D-glucuronide, baicalin, and 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide Streptococcus sp. ? - ? 89 3.2.1.31 additional information substrate specificity with flavonoids and phytoestrogens of crude and purified enzyme from digestive juice, overview Helix pomatia ? - ? 89 3.2.1.31 additional information enzyme additionally catalyzes the transglycosylation of glucuronic acid residues from 4-nitrophenyl beta-D-glucuronic acid to various monosaccharide acceptors such as glucose, galactose, and xylose Aspergillus niger ? - ? 89 3.2.1.31 additional information enzyme additionally catalyzes the transglycosylation of glucuronic acid residues from 4-nitrophenyl beta-D-glucuronic acid to various monosaccharide acceptors such as glucose, galactose, and xylose Neurospora crassa ? - ? 89 3.2.1.31 additional information the beta-glucuronidase forms a complex with esterase 22 in the liver. Gus colocalizes with Es22 at the endoplasmic reticulum but does not affect its retinoyl ester hydrolase activity Mus musculus ? - ? 89 3.2.1.31 additional information the enzyme does not show strict specificity toward the aglycone moiety and exhibits activity toward all the natural and artificial substrates. Strict glycan specificity and hydrolyzes only the artificial substrate containing glucuronide groups Aspergillus oryzae ? - ? 89 3.2.1.31 additional information when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed Escherichia coli HGU-3 ? - ? 89 3.2.1.31 additional information the enzyme does not show strict specificity toward the aglycone moiety and exhibits activity toward all the natural and artificial substrates. Strict glycan specificity and hydrolyzes only the artificial substrate containing glucuronide groups Aspergillus oryzae Li-3 ? - ? 89 3.2.1.31 additional information no activity of isozymes I and II with 4-nitrophenyl beta-D-glucuronide, baicalin, and 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide Streptococcus sp. LJ-22 ? - ? 89 3.2.1.31 N-([[4-(beta-D-glucopyranuronosyloxy)-3-nitrobenzyl]oxy]carbonyl)-N-methyl-L-valyl-N-[(3R,4S,5S)-1-[(2S)-2-[(1R,2R)-3-[[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino]-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl]-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-met + H2O - Homo sapiens D-glucuronate + N-[[(4-hydroxy-3-nitrobenzyl)oxy]carbonyl]-N-methyl-L-valyl-N-[(3R,4S,5S)-1-[(2S)-2-[(1R,2R)-3-[[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino]-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl]-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-methyl-L-valinamide - ? 430439 3.2.1.31 naphthol AS-BI-D-glucuronide + H2O - Mammalia ? - ? 13967 3.2.1.31 o-nitrophenyl-beta-D-glucuronide + H2O - Escherichia coli o-nitrophenol + D-glucuronic acid - ? 393028 3.2.1.31 oestrone 3-glucuronide + H2O - Sus scrofa ? - ? 369528 3.2.1.31 p-nitrophenyl-beta-D-glucuronide + H2O - Escherichia coli p-nitrophenol + D-glucuronate - ? 13764 3.2.1.31 p-nitrophenyl-beta-D-glucuronide + H2O - Escherichia coli HGU-3 p-nitrophenol + D-glucuronate - ? 13764 3.2.1.31 p-nitrophenyl-beta-D-glucuronide + H2O 1% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 192% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ? - ? 374220 3.2.1.31 p-nitrophenyl-beta-D-glucuronide + H2O - Aspergillus niger p-nitrophenol + beta-D-glucuronic acid - ? 374221 3.2.1.31 p-nitrophenyl-beta-D-glucuronide + H2O - Lactobacillus gasseri p-nitrophenol + beta-D-glucuronic acid - ? 374221 3.2.1.31 p-nitrophenyl-beta-D-glucuronide + H2O - Lactobacillus gasseri ADH p-nitrophenol + beta-D-glucuronic acid - ? 374221 3.2.1.31 p-nitrophenyl-beta-D-glucuronide + H2O - Escherichia coli p-nitrophenol + D-glucuronic acid - ? 393127 3.2.1.31 phenolphthalein beta-D-glucuronide + H2O determination of specific activity in betaG-expressing tumour CT26 cells using radio-labeled substrate Mus musculus phenolphthalein + beta-D-glucuronate - ? 412681 3.2.1.31 phenolphthalein beta-D-glucuronide + H2O - Mus musculus phenolphthalein + D-glucuronic acid - ? 452769 3.2.1.31 phenolphthalein beta-D-glucuronide + H2O - Homo sapiens phenolphthalein + D-glucuronic acid - ? 452769 3.2.1.31 phenolphthalein beta-D-glucuronide + H2O - Littorina littorea phenolphthalein + D-glucuronic acid - ? 452769 3.2.1.31 phenolphthalein-beta-D-glucuronic acid + H2O - Labeo rohita phenolphthalein + D-glucuronic acid - ir 412682 3.2.1.31 phenolphthalein-beta-D-glucuronide + H2O - Mus musculus phenolphthalein + D-glucuronic acid - ? 13960 3.2.1.31 phenolphthalein-beta-D-glucuronide + H2O - Escherichia coli phenolphthalein + D-glucuronic acid - ? 13960 3.2.1.31 phenolphthalein-beta-D-glucuronide + H2O - Homo sapiens phenolphthalein + D-glucuronic acid - ? 13960 3.2.1.31 phenolphthalein-beta-D-glucuronide + H2O - Littorina littorea phenolphthalein + D-glucuronic acid - ? 13960 3.2.1.31 quercetin 3-O-beta-D-glucuronide + H2O - Homo sapiens quercetin + D-glucuronate - ? 406541 3.2.1.31 quercetin 4'-O-beta-D-glucuronide + H2O - Homo sapiens quercetin + beta-D-glucuronate - ? 452771 3.2.1.31 quercetin 7-O-beta-D-glucuronide + H2O - Homo sapiens quercetin + D-glucuronate - ? 406543 3.2.1.31 quercetin-4'-O-glucuronide + H2O - Homo sapiens quercetin + D-glucuronate - ? 406542 3.2.1.31 retinyl-beta-glucuronide + H2O - Mus musculus retinol + D-glucuronide - ? 410988 3.2.1.31 sodium (p-nitrophenyl beta-D-glucopyranoside)uronate + p-nitrophenyl alpha-D-galactopyranoside the enzyme shows transglucuronidation activity: when (p-nitrophenyl beta-D-glucopyranoside)uronate is used as a donor and p-nitrophenyl alpha-D-galactopyranoside is used as an acceptor, p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-4)-alpha-D-galactopyranoside and p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-2)-alpha-D-galactopyranoside are synthesized in yields of 16% and 21% respectively Bos taurus p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-4)-alpha-D-galactopyranoside + p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-2)-alpha-D-galactopyranoside - ? 374640 3.2.1.31 wogonin 7-O-beta-D-glucuronide + H2O - Levilactobacillus brevis wogonin + beta-D-glucuronate - ? 413061 3.2.1.31 wogonin 7-O-beta-D-glucuronide + H2O - Levilactobacillus brevis RO1 wogonin + beta-D-glucuronate - ? 413061