3.2.1.167 4-methylumbelliferyl-beta-D-glucuronide + H2O - Scutellaria baicalensis 4-methylumbelliferol + D-glucuronate - ? 411325 3.2.1.167 4-nitrophenyl beta-D-glucuronide + H2O - Levilactobacillus brevis 4-nitrophenol + D-glucuronate - ? 372060 3.2.1.167 4-nitrophenyl beta-D-glucuronide + H2O - Levilactobacillus brevis FERM BP-4693 4-nitrophenol + D-glucuronate - ? 372060 3.2.1.167 4-nitrophenyl beta-D-glucuronide + H2O - Scutellaria viscidula 4-nitrophenol + beta-D-glucuronic acid - ? 381184 3.2.1.167 4-nitrophenyl beta-D-glucuronide + H2O - Levilactobacillus brevis 4-nitrophenol + beta-D-glucuronic acid - ? 381184 3.2.1.167 4-phenolphthaleinyl beta-D-glucuronide + H2O - Scutellaria viscidula 4-phenolphthalein + beta-D-glucuronic acid - ? 381219 3.2.1.167 5,6,7-trihydroxyflavone-7-beta-D-glucuronide + H2O - Escherichia coli 5,6,7-trihydroxyflavone + D-glucuronate - ? 414145 3.2.1.167 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O i.e. baicalin Scutellaria baicalensis 5,6,7-trihydroxyflavone + D-glucuronate i.e. baicalein ? 408193 3.2.1.167 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O i.e. baicalin. No activity with p-nitrophenyl-beta-D-glucoside. Activity with p-nitrophenyl-beta-D-glucuronide is about 4% of the activity with 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid (baicalin), activity with p-phenolphthalein-beta-D-glucuronide is 9.5% of the activity with 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid (baicalin) Scutellaria viscidula 5,6,7-trihydroxyflavone + D-glucuronate i.e. baicalein ? 408193 3.2.1.167 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O i.e. baicalin. The enzyme shows more than 20fold higher activity for baicalein 7-O-beta-D-glucuronide as compared with luteolin 3'-O-beta-D-glucuronide Scutellaria baicalensis 5,6,7-trihydroxyflavone + D-glucuronate i.e. baicalein ? 408193 3.2.1.167 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O i.e. baicalin. No activity with p-nitrophenyl-beta-D-glucoside. Activity with p-nitrophenyl-beta-D-glucuronide is about 4% of the activity with 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid (baicalin), activity with p-phenolphthalein-beta-D-glucuronide is 9.5% of the activity with 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid (baicalin) Scutellaria viscidula Bge 5,6,7-trihydroxyflavone + D-glucuronate i.e. baicalein ? 408193 3.2.1.167 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O i.e. baicalin Scutellaria baicalensis Georgi 5,6,7-trihydroxyflavone + D-glucuronate i.e. baicalein ? 408193 3.2.1.167 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O i.e. baicalin Scutellaria baicalensis Georg 5,6,7-trihydroxyflavone + D-glucuronate i.e. baicalein ? 408193 3.2.1.167 5,7-dihydroxy-6-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. oroxylin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid Scutellaria baicalensis 5,7-dihydroxy-6-methoxyflavone + D-glucuronate - ? 408197 3.2.1.167 5,7-dihydroxy-6-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. oroxylin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-6-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid Scutellaria baicalensis Georgi 5,7-dihydroxy-6-methoxyflavone + D-glucuronate - ? 408197 3.2.1.167 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. wogonin 7-O-beta-D-glucuronide Scutellaria viscidula 5,7-dihydroxy-8-methoxyflavone + D-glucuronate - ? 408198 3.2.1.167 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. wogonin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid Scutellaria baicalensis 5,7-dihydroxy-8-methoxyflavone + D-glucuronate - ? 408198 3.2.1.167 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. wogonin 7-O-beta-D-glucuronide Scutellaria viscidula Bge 5,7-dihydroxy-8-methoxyflavone + D-glucuronate - ? 408198 3.2.1.167 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. wogonin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid Scutellaria baicalensis Georgi 5,7-dihydroxy-8-methoxyflavone + D-glucuronate - ? 408198 3.2.1.167 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. wogonin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid Scutellaria baicalensis Georg 5,7-dihydroxy-8-methoxyflavone + D-glucuronate - ? 408198 3.2.1.167 baicalin + H2O - Mus musculus baicalein + D-glucuronate - ? 372494 3.2.1.167 baicalin + H2O - Escherichia coli baicalein + D-glucuronate - ? 372494 3.2.1.167 baicalin + H2O - Helix pomatia baicalein + D-glucuronate - ? 372494 3.2.1.167 baicalin + H2O - Scutellaria baicalensis baicalein + D-glucuronate - ? 372494 3.2.1.167 baicalin + H2O 0.15 mM of baicalin is completely transformed into baicalein within 3 h at 37°C, pH 5.0 Levilactobacillus brevis baicalein + D-glucuronate - ? 372494 3.2.1.167 baicalin + H2O the enzyme prefers baicalin rather than estrone 3-(beta-D-glucuronide), one of the human endogenous steroid hormones Levilactobacillus brevis baicalein + D-glucuronate - ? 372494 3.2.1.167 baicalin + H2O the enzyme is strictly glycon-specific but not aglycon-specific Levilactobacillus brevis baicalein + D-glucuronate - ? 372494 3.2.1.167 baicalin + H2O baicalein 7-O-beta-D-glucuronide Helix pomatia baicalein + D-glucuronate - ? 372494 3.2.1.167 baicalin + H2O the enzyme prefers baicalin rather than estrone 3-(beta-D-glucuronide), one of the human endogenous steroid hormones Levilactobacillus brevis FERM BP-4693 baicalein + D-glucuronate - ? 372494 3.2.1.167 baicalin + H2O the enzyme is strictly glycon-specific but not aglycon-specific Levilactobacillus brevis FERM BP-4693 baicalein + D-glucuronate - ? 372494 3.2.1.167 baicalin + H2O - Scutellaria viscidula baicalein + beta-D-glucuronic acid - ? 380563 3.2.1.167 baicalin + H2O best substrate Scutellaria viscidula baicalein + beta-D-glucuronic acid - ? 380563 3.2.1.167 estrone 3-(beta-D-glucuronide) + H2O - Levilactobacillus brevis estrone + D-glucuronate - ? 431842 3.2.1.167 estrone 3-(beta-D-glucuronide) + H2O - Levilactobacillus brevis FERM BP-4693 estrone + D-glucuronate - ? 431842 3.2.1.167 additional information no activity with 4-nitrophenyl beta-D-glucoside Scutellaria viscidula ? - ? 89 3.2.1.167 additional information no activity with 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide and luteolin 3'-O-beta-D-glucuronide Scutellaria baicalensis ? - ? 89 3.2.1.167 additional information the enzyme is not able to cleave the bond between the two glucuronic acid moieties in glycyrrhizin (glycyrrhetic acid 3-O-D-diglucuronide), the enzyme is not capable of hydrolyzing glucosides, luteolin-7-glucoside and apigenin-7-O-glucoside Scutellaria baicalensis ? - ? 89 3.2.1.167 additional information substrate specificity, the enzyme is inactive with other 4-nitrophenyl glycosides, overview Levilactobacillus brevis ? - ? 89 3.2.1.167 additional information domination of the glucuronide wogonoside is a characteristic feature of the hairy roots culture, while glucuronide baicalin is predominant in intact plant roots and also in the hairy roots. Alteration in contents of the main flavones in hairy roots culture of the Baikal skullcap during culturing, overview Scutellaria baicalensis ? - ? 89 3.2.1.167 additional information development of a chemically defined medium-based baicalein bioproduction process with a comparable yield compared to complex medium-based ones, detailed overview Helix pomatia ? - ? 89 3.2.1.167 additional information no activity with 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide and luteolin 3'-O-beta-D-glucuronide Scutellaria baicalensis Georgi ? - ? 89 3.2.1.167 additional information substrate specificity, the enzyme is inactive with other 4-nitrophenyl glycosides, overview Levilactobacillus brevis FERM BP-4693 ? - ? 89 3.2.1.167 additional information the enzyme is not able to cleave the bond between the two glucuronic acid moieties in glycyrrhizin (glycyrrhetic acid 3-O-D-diglucuronide), the enzyme is not capable of hydrolyzing glucosides, luteolin-7-glucoside and apigenin-7-O-glucoside Scutellaria baicalensis Georg ? - ? 89 3.2.1.167 wogonoside + H2O 0.125 mM of wogonoside is completely transformed into wogonin within 3 h at 37°C, pH 5.0 Levilactobacillus brevis wogonin + ? - ? 415448 3.2.1.167 wogonoside + H2O - Levilactobacillus brevis ? - ? 432474 3.2.1.167 wogonoside + H2O - Scutellaria baicalensis wogonin + D-glucuronate - ? 449024