3.2.1.165 4-methylumbelliferyl 2-amino-2-deoxy-beta-D-glucopyranoside + H2O - Amycolatopsis orientalis 4-methylumbelliferone + 2-amino-2-deoxy-beta-D-glucopyranose - ? 408113 3.2.1.165 4-methylumbelliferyl beta-D-glucoside + H2O - Thermococcus kodakarensis 4-methylumbelliferol + D-glucose - ? 390832 3.2.1.165 4-nitrophenyl beta-D-glucosaminide + H2O - Photobacterium profundum 4-nitrophenol + beta-D-glucosamine - ? 416375 3.2.1.165 4-nitrophenyl beta-D-glucoside + H2O - Photobacterium profundum 4-nitrophenol + beta-D-glucose - ? 364700 3.2.1.165 4-nitrophenyl beta-D-glucoside + H2O - Photobacterium profundum SS9 4-nitrophenol + beta-D-glucose - ? 364700 3.2.1.165 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O - Penicillium sp. IB-37-2A ? - ? 418310 3.2.1.165 alpha-D-glucosaminyl fluoride + D-glucosamine - Photobacterium profundum chitobiose + fluoride - ? 446960 3.2.1.165 alpha-D-glucosaminyl fluoride + D-glucosamine - Photobacterium profundum SS9 chitobiose + fluoride - ? 446960 3.2.1.165 beta-1,4-D-glucosamine tetrasaccharide tetrasaccharide of chitosan efficiently depolymerizes chitosan to its constituent p-glucosamine monosaccharide in cooperation with endo-chitosanase Amycolatopsis orientalis D-glucosamine + chitotriose - ? 402508 3.2.1.165 beta-glucan + H2O 13.6% activity compared to soluble chitosan Penicillium sp. IB-37-2A ? - ? 94321 3.2.1.165 cellobiose + H2O - Photobacterium profundum ? - ? 372639 3.2.1.165 cellobiose + H2O - Photobacterium profundum SS9 ? - ? 372639 3.2.1.165 cellopentaose + H2O - Photobacterium profundum ? - ? 372650 3.2.1.165 cellopentaose + H2O - Photobacterium profundum SS9 ? - ? 372650 3.2.1.165 cellotetraose + H2O - Photobacterium profundum ? - ? 372657 3.2.1.165 cellotriose + H2O - Photobacterium profundum ? - ? 94515 3.2.1.165 cellotriose + H2O - Photobacterium profundum SS9 ? - ? 94515 3.2.1.165 chitin + H2O - Aspergillus fumigatus ? - ? 115729 3.2.1.165 chitin + H2O - Aspergillus fumigatus IIT-004 ? - ? 115729 3.2.1.165 chitobiitol + H2O 0.3% of the activity with chitopentaose Amycolatopsis orientalis ? - ? 391621 3.2.1.165 chitobiose + H2O - Pyrococcus horikoshii 2 D-glucosamine - ? 259772 3.2.1.165 chitobiose + H2O - Thermococcus kodakarensis 2 D-glucosamine - ? 259772 3.2.1.165 chitobiose + H2O - Photobacterium profundum ? - ? 364721 3.2.1.165 chitobiose + H2O - Photobacterium profundum SS9 ? - ? 364721 3.2.1.165 chitobiose + H2O - Aspergillus flavus D-glucosamine - ? 391622 3.2.1.165 chitobiose + H2O - Aspergillus fumigatus D-glucosamine - ? 391622 3.2.1.165 chitobiose + H2O - Talaromyces funiculosus D-glucosamine - ? 391622 3.2.1.165 chitobiose + H2O - Amycolatopsis orientalis D-glucosamine - ? 391622 3.2.1.165 chitobiose + H2O - Photobacterium profundum D-glucosamine - ? 391622 3.2.1.165 chitobiose + H2O - Thermococcus kodakarensis D-glucosamine - ? 391622 3.2.1.165 chitobiose + H2O 38% of the activity with chitopentaose Amycolatopsis orientalis D-glucosamine - ? 391622 3.2.1.165 chitobiose + H2O - Talaromyces funiculosus KY616 D-glucosamine - ? 391622 3.2.1.165 chitobiose + H2O - Aspergillus flavus IAM2044 D-glucosamine - ? 391622 3.2.1.165 chitobiose + H2O - Aspergillus fumigatus S-26 D-glucosamine - ? 391622 3.2.1.165 chitobiose + H2O (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product Purpureocillium lilacinum glucosamine + glucosamine - ? 430032 3.2.1.165 chitoheptaose + H2O products at an early stage of reaction Aspergillus fumigatus D-glucosamine + chitohexaose - ? 391623 3.2.1.165 chitoheptaose + H2O products at an early stage of reaction Aspergillus fumigatus S-26 D-glucosamine + chitohexaose - ? 391623 3.2.1.165 chitohexaitol + H2O 94% of the activity with chitopentaose Amycolatopsis orientalis ? - ? 391624 3.2.1.165 chitohexaitol + H2O 89% of the activity with chitopentaitol Penicillium sp. D-glucosamine + ? - ? 391625 3.2.1.165 chitohexaitol + H2O 89% of the activity with chitopentaitol Penicillium sp. AF9-P-112 D-glucosamine + ? - ? 391625 3.2.1.165 chitohexaose + H2O 92% of the activity with chitopentaose Amycolatopsis orientalis D-glucosamine - ? 391626 3.2.1.165 chitohexaose + H2O at the early stages of the reaction, chitohexaose is converted to D-glucosamine and chitopentaose Thermococcus kodakarensis D-glucosamine - ? 391626 3.2.1.165 chitohexaose + H2O chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine Aspergillus flavus D-glucosamine - ? 391626 3.2.1.165 chitohexaose + H2O cleaved to chitopentaose and glusosamine in the initial stage of the reaction Trichoderma reesei D-glucosamine - ? 391626 3.2.1.165 chitohexaose + H2O cleaved to chitopentaose and glusosamine in the initial stage of the reaction Trichoderma reesei PC-3-7 D-glucosamine - ? 391626 3.2.1.165 chitohexaose + H2O chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine Aspergillus flavus IAM2044 D-glucosamine - ? 391626 3.2.1.165 chitohexaose + H2O - Amycolatopsis orientalis D-glucosamine + ? - ? 391627 3.2.1.165 chitohexaose + H2O chitohexaose is hydrolyzed to chitopentaose + D-glucosamine at the beginning, and then the resulting chitopentaose changes successively to a smaller chitooligosaccharide with concomitant brelease of D-glucosamine Aspergillus oryzae D-glucosamine + ? - ? 391627 3.2.1.165 chitohexaose + H2O chitohexaose is hydrolyzed to chitopentaose + D-glucosamine at the beginning, and then the resulting chitopentaose changes successively to a smaller chitooligosaccharide with concomitant brelease of D-glucosamine Aspergillus oryzae IAM2660 D-glucosamine + ? - ? 391627 3.2.1.165 chitohexaose + H2O chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine Aspergillus fumigatus D-glucosamine + chitopentaose - ? 391628 3.2.1.165 chitohexaose + H2O chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine Aspergillus fumigatus S-26 D-glucosamine + chitopentaose - ? 391628 3.2.1.165 chitohexaose + H2O GlcNn: polymerization degree of n=2-6, chitosan oligosaccharides, 0.96 nM exochiosanase, 10 mM ammonium acetate, pH 5.2, substrate concentrations: 6.25, 12.5, 25, 37.5 microM, hexa-N-acetylchitohexaose as control substrate at 25 microM Amycolatopsis orientalis 2-amino-2-deoxy-D-glucopyranose + chitopentaose - ? 404654 3.2.1.165 chitohexaose + H2O (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product Purpureocillium lilacinum chitopentaose + glucosamine - ? 430035 3.2.1.165 chitopentaitol + H2O 99% of the activity with chitopentaose Amycolatopsis orientalis ? - ? 391629 3.2.1.165 chitopentaitol + H2O - Penicillium sp. D-glucosamine + ? - ? 391630 3.2.1.165 chitopentaitol + H2O - Penicillium sp. AF9-P-112 D-glucosamine + ? - ? 391630 3.2.1.165 chitopentaose + H2O cleaved to chitotetraose and glucosamine in the initial stage of the reaction Trichoderma reesei ? - ? 115501 3.2.1.165 chitopentaose + H2O cleaved to chitotetraose and glucosamine in the initial stage of the reaction Trichoderma reesei PC-3-7 ? - ? 115501 3.2.1.165 chitopentaose + H2O - Aspergillus flavus D-glucosamine - ? 391631 3.2.1.165 chitopentaose + H2O - Trichoderma reesei D-glucosamine - ? 391631 3.2.1.165 chitopentaose + H2O - Amycolatopsis orientalis D-glucosamine - ? 391631 3.2.1.165 chitopentaose + H2O at the early stages of the reaction, chitopentaose is converted to D-glucosamine and chitotetraose Thermococcus kodakarensis D-glucosamine - ? 391631 3.2.1.165 chitopentaose + H2O - Trichoderma reesei PC-3-7 D-glucosamine - ? 391631 3.2.1.165 chitopentaose + H2O - Aspergillus flavus IAM2044 D-glucosamine - ? 391631 3.2.1.165 chitopentaose + H2O - Amycolatopsis orientalis D-glucosamine + ? - ? 391632 3.2.1.165 chitopentaose + H2O products at an early stage of reaction Aspergillus fumigatus D-glucosamine + chitotetraose - ? 391633 3.2.1.165 chitopentaose + H2O products at an early stage of reaction Aspergillus fumigatus S-26 D-glucosamine + chitotetraose - ? 391633 3.2.1.165 chitopentaose + H2O (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product Purpureocillium lilacinum chitotetraose + glucosamine - ? 430039 3.2.1.165 chitosan + H2O - Chryseobacterium indologenes ? - ? 259769 3.2.1.165 chitosan + H2O - Amycolatopsis orientalis ? - ? 259769 3.2.1.165 chitosan + H2O - Comamonas koreensis ? - ? 259769 3.2.1.165 chitosan + H2O 90% of the activity with chitopentaose Amycolatopsis orientalis ? - ? 259769 3.2.1.165 chitosan + H2O the exo-beta-D-glucosaminidase cleaves the glycosidic link of either GlcN-beta(1,4)-GlcN or GlcN-beta(1,4)-GlcNAc Trichoderma reesei ? - ? 259769 3.2.1.165 chitosan + H2O the exo-beta-D-glucosaminidase cleaves the glycosidic link of either GlcN-beta(1,4)-GlcN or GlcN-beta(1,4)-GlcNAc Trichoderma reesei PC-3-7 ? - ? 259769 3.2.1.165 chitosan + H2O - Chryseobacterium indologenes HANYOO ? - ? 259769 3.2.1.165 chitosan + H2O - Comamonas koreensis HANWOO ? - ? 259769 3.2.1.165 chitosan + H2O - Aspergillus flavus D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O - Aspergillus fumigatus D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O - Trichoderma reesei D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O - Amycolatopsis orientalis D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O fully deacetylated chitosan Aspergillus fumigatus D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O only D-glucosamine is produced after prolonged hydrolysis Talaromyces funiculosus D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O only glucosamine is produced as final product after prolonged reaction Aspergillus oryzae D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O soluble chitosan, powdered chitosan and colloidal chitosan Penicillium sp. D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O 100% activity with 95% deacetylated chitosan, 88% activity with 83% deacetylated chitosan, 80% activity with 70% deacetylated chitosan, and 68% activity with 60% deacetylated chitosan Aspergillus sp. D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O chitosan 10B Trichoderma reesei D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O chitosan with more than 50% degree of acetylation, 9% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation Aspergillus fumigatus D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O chitosan with more than 60% degree of acetylation, 21% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation Aspergillus fumigatus D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O chitosan with more than 70% degree of acetylation, 43% activity compared to crab shell chitosan with more than 90% degree of acetylation Aspergillus fumigatus D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O chitosan with more than 80% degree of acetylation, 68% activity compared to crab shell chitosan with more than 90% degree of acetylation Aspergillus fumigatus D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O soluble chitosan, powdered chitosan and colloidal chitosan Penicillium sp. AF9-P-112 D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O only D-glucosamine is produced after prolonged hydrolysis Talaromyces funiculosus KY616 D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O - Aspergillus fumigatus IIT-004 D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O chitosan with more than 50% degree of acetylation, 9% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation Aspergillus fumigatus IIT-004 D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O chitosan with more than 60% degree of acetylation, 21% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation Aspergillus fumigatus IIT-004 D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O - Aspergillus flavus IAM2044 D-glucosamine + ? - ? 391637 3.2.1.165 chitosan + H2O only glucosamine is produced as final product after prolonged reaction Aspergillus oryzae IAM2660 D-glucosamine + ? - ? 391637 3.2.1.165 chitotetraitol + H2O as active as chitopentaose Amycolatopsis orientalis ? - ? 391640 3.2.1.165 chitotetraitol + H2O 97% of the activity with chitopentaitol Penicillium sp. D-glucosamine + ? - ? 391641 3.2.1.165 chitotetraitol + H2O 97% of the activity with chitopentaitol Penicillium sp. AF9-P-112 D-glucosamine + ? - ? 391641 3.2.1.165 chitotetraose + H2O - Amycolatopsis orientalis ? - ? 364722 3.2.1.165 chitotetraose + H2O - Aspergillus flavus D-glucosamine - ? 391642 3.2.1.165 chitotetraose + H2O - Talaromyces funiculosus D-glucosamine - ? 391642 3.2.1.165 chitotetraose + H2O 99% of the activity with chitopentaose Amycolatopsis orientalis D-glucosamine - ? 391642 3.2.1.165 chitotetraose + H2O at first, the enzyme predominantly produces D-glucosamine and chitotriose, which is further degraded into D-glucosamine and chitobiose. The transglycosylation product, chitopentaose, is also produced, together with a lesser amount of chitohexaose Amycolatopsis orientalis D-glucosamine - ? 391642 3.2.1.165 chitotetraose + H2O at the early stages of the reaction, chitotetraose is converted to D-glucosamine and chitotriose Thermococcus kodakarensis D-glucosamine - ? 391642 3.2.1.165 chitotetraose + H2O - Talaromyces funiculosus KY616 D-glucosamine - ? 391642 3.2.1.165 chitotetraose + H2O products at an early stage of reaction Aspergillus fumigatus D-glucosamine + chitotriose - ? 391643 3.2.1.165 chitotetraose + H2O (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product Purpureocillium lilacinum chitotriose + glucosamine - ? 430047 3.2.1.165 chitotriitol + H2O 85% of the activity with chitopentaitol Penicillium sp. D-glucosamine + ? - ? 391644 3.2.1.165 chitotriitol + H2O 85% of the activity with chitopentaose Amycolatopsis orientalis ? - ? 391645 3.2.1.165 chitotriitol + H2O - Talaromyces funiculosus D-glucosamine - ? 391646 3.2.1.165 chitotriitol + H2O - Talaromyces funiculosus KY616 D-glucosamine - ? 391646 3.2.1.165 chitotriose + H2O - Amycolatopsis orientalis ? - ? 381653 3.2.1.165 chitotriose + H2O - Aspergillus flavus D-glucosamine - ? 391647 3.2.1.165 chitotriose + H2O - Thermococcus kodakarensis D-glucosamine - ? 391647 3.2.1.165 chitotriose + H2O 92% of the activity with chitopentaose Amycolatopsis orientalis D-glucosamine - ? 391647 3.2.1.165 chitotriose + H2O products at an early stage of reaction Aspergillus fumigatus D-glucosamine + chitobiose - ? 391648 3.2.1.165 chitotriose + H2O - Talaromyces funiculosus chitobiose + D-glucosamine - ? 430049 3.2.1.165 chitotriose + H2O (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product Purpureocillium lilacinum chitobiose + D-glucosamine - ? 430049 3.2.1.165 colloidal chitin + H2O deacetylation degree 80-85%, 6.4% activity compared to soluble chitosan Penicillium sp. IB-37-2A D-glucosamine + ? - ? 447337 3.2.1.165 colloidal chitosan + H2O - Aspergillus oryzae beta-D-glucosamine + ? - ? 447338 3.2.1.165 colloidal chitosan + H2O - Aspergillus oryzae FL402 beta-D-glucosamine + ? - ? 447338 3.2.1.165 colloidal chitosan + H2O 56% activity compared to crab shell chitosan with more than 90% degree of acetylation Aspergillus fumigatus D-glucosamine + ? - ? 447339 3.2.1.165 crab shell chitosan + H2O more than 90% degree of acetylation, 100% activity Aspergillus fumigatus D-glucosamine + ? - ? 447350 3.2.1.165 D-glucosamine + N-acetyl-D-glucosamine 15% (w/v) ammonium sulfate is optimum for catalyzing transglycosylation reaction Aspergillus fumigatus chitodimer - ? 447376 3.2.1.165 deacetylated chitosan + H2O slight hydrolysis Photobacterium profundum ? - ? 373019 3.2.1.165 GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O - Photobacterium profundum GlcN(beta1-4)GlcN + D-glucosamine - ? 417038 3.2.1.165 GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O - Photobacterium profundum GlcN(beta1-4)GlcN(beta1-4)GlcN + D-glucosamine - ? 417039 3.2.1.165 GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O - Photobacterium profundum GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + D-glucosamine - ? 417040 3.2.1.165 GlcNbeta(1-4)GlcNAc + H2O - Amycolatopsis orientalis D-glucosamine + N-acetylglucosamine - ? 392077 3.2.1.165 GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNAc + H2O (GlcN)3-GlcNAc (mono-N-acetylated chitotetraose, the reducing end residue is N-acetylated) is at first degraded into (GlcN)2-GlcNAc, and then into GlcN-GlcNAc. After a longer incubation period, the substrate is finally hydrolyzed into monosaccharides, GlcN and GlcNAc Amycolatopsis orientalis GlcNbeta(1-4)GlcNbeta(1-4)GlcNAc + D-glucosamine - ? 392080 3.2.1.165 GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNAc + H2O (GlcN)3-GlcNAc is a mono-N-acetylated chitotetraose, the reducing end residue is N-acetylated. D-glucosamine is produced from the nonreducing end together with the transglycosylation products Amycolatopsis orientalis GlcNbeta(1-4)GlcNbeta(1-4)GlcNAc + D-glucosamine - ? 392080 3.2.1.165 glycol chitosan + H2O - Aspergillus flavus ? - ? 95085 3.2.1.165 glycol chitosan + H2O 4% of the activity with chitosan Aspergillus oryzae ? - ? 95085 3.2.1.165 glycol chitosan + H2O - Aspergillus flavus IAM2044 ? - ? 95085 3.2.1.165 glycol chitosan + H2O 4% of the activity with chitosan Aspergillus oryzae IAM2660 ? - ? 95085 3.2.1.165 glycol chitosan + H2O - Penicillium sp. D-glucosamine + ? - ? 392145 3.2.1.165 glycol chitosan + H2O 12% activity compared to crab shell chitosan with more than 90% degree of acetylation Aspergillus fumigatus D-glucosamine + ? - ? 392145 3.2.1.165 glycol chitosan + H2O 631.3% activity compared to soluble chitosan Penicillium sp. IB-37-2A D-glucosamine + ? - ? 392145 3.2.1.165 laminarin + H2O 21.5% activity compared to soluble chitosan Penicillium sp. IB-37-2A ? - ? 94671 3.2.1.165 additional information no activity is detectable against p-nitrophenyl derivatives of beta-D-glucopyranoside, beta-D-xylopyranoside, beta-D-mannoside, beta-D-glucuronide, beta-D-galactoside, beta-D-N-acetylgalactosaminide, beta-D-N-acetylglucosaminide and alpha-D-N-acetylglucosaminide Amycolatopsis orientalis ? - ? 89 3.2.1.165 additional information no degradation of cellulose and starch. The exo-beta-D-glucosaminidase exhibits transglycosylation activity, resulting in the one-residue elongated oligomers Aspergillus fumigatus ? - ? 89 3.2.1.165 additional information the enzyme also catalyzes transglycosylation reactions Talaromyces funiculosus ? - ? 89 3.2.1.165 additional information the enzyme catalyzes transglycosylation reaction on chitobiose and chitotriose Penicillium sp. ? - ? 89 3.2.1.165 additional information Chitosanases hydrolyze beta-1,4-glycosidic linkage of chitosan, a polysaccharide consisting mainly of D-glucosamine with a variable content of N-acetyl-D-glucosamine Amycolatopsis orientalis ? - ? 89 3.2.1.165 additional information cleaving nonreducing beta-D-glucosamine residues of oligosaccharides, highly specific due to pocket for N2 group of glucosaminidase discriminating it from glucose Amycolatopsis orientalis ? - ? 89 3.2.1.165 additional information no hydrolysis of colloid chitin and carboxymethylcellulose Aspergillus sp. ? - ? 89 3.2.1.165 additional information the enzyme fails to release a reducing sugar from avicel and carboxylmethyl cellulose Photobacterium profundum ? - ? 89 3.2.1.165 additional information the enzyme acts on beta-1,4-linkage of chitosan from its non-reducing end and produces D-glucosamine as the exclusive end product Penicillium decumbens ? - ? 89 3.2.1.165 additional information preparation of alpha-chitin and chitosan from shrimp processing raw byproducts Penicillium decumbens ? - ? 89 3.2.1.165 additional information the enzyme cleaves the beta-1->4-glycosidic bonds between GlcN-GlcN and GlcN-GlcNAc but not between GlcNAc-GlcN or GlcNAc-GlcNAc, cleavage of GlcN-GlcNAc, a partially acetylated chitooligomer mixture, overview. The purified enzyme displays high activity toward chitosan and (GlcN)6. No significant activity with N-carboxymethyl chitosan, or chitin, also synthetic p-nitrophenyl-beta-D-glucosaminide is not hydrolyzed. Chitosan with higher deacetylation degrees is hydrolyzed more efficiently than chitosan with lower deacetylation values. Substrate specifiicty analysis using massspectrometry. Paecilomyces exo-beta-D-GlcNase can accelerate the hydrolysis of p-nitrophenyl-beta-D-N-acetylglucosaminide, p-nitrophenyl-beta-D-N,N'-diacetylchitobioside, and p-nitrophenyl-beta-D-N-acetylgalactosaminide to release the corresponding sugar Purpureocillium lilacinum ? - ? 89 3.2.1.165 additional information no activity with carboxymethyl cellulose, cellulose, xylan and galactomannan Penicillium sp. IB-37-2A ? - ? 89 3.2.1.165 additional information no activity with flaked chitin, colloidal chitin and cellulose Aspergillus fumigatus ? - ? 89 3.2.1.165 additional information the enzyme catalyzes transglycosylation reaction on chitobiose and chitotriose Penicillium sp. AF9-P-112 ? - ? 89 3.2.1.165 additional information the enzyme also catalyzes transglycosylation reactions Talaromyces funiculosus KY616 ? - ? 89 3.2.1.165 additional information no activity with flaked chitin, colloidal chitin and cellulose Aspergillus fumigatus IIT-004 ? - ? 89 3.2.1.165 additional information the enzyme acts on beta-1,4-linkage of chitosan from its non-reducing end and produces D-glucosamine as the exclusive end product Penicillium decumbens CFRNT15 ? - ? 89 3.2.1.165 additional information preparation of alpha-chitin and chitosan from shrimp processing raw byproducts Penicillium decumbens CFRNT15 ? - ? 89 3.2.1.165 additional information no degradation of cellulose and starch. The exo-beta-D-glucosaminidase exhibits transglycosylation activity, resulting in the one-residue elongated oligomers Aspergillus fumigatus S-26 ? - ? 89 3.2.1.165 additional information the enzyme fails to release a reducing sugar from avicel and carboxylmethyl cellulose Photobacterium profundum SS9 ? - ? 89 3.2.1.165 p-nitrophenyl beta-D-glucosaminide + H2O - Amycolatopsis orientalis p-nitrophenol + beta-D-glucosamine - ? 393086 3.2.1.165 p-nitrophenyl-beta-D-glucosamine + H2O - Amycolatopsis orientalis D-glucosamine + p-nitrophenol - ? 406305 3.2.1.165 p-nitrophenyl-beta-D-N-acetylglucosaminide + H2O - Trichoderma reesei p-nitrophenol + beta-D-N-acetylglucosamine - ? 374229 3.2.1.165 p-nitrophenyl-beta-D-N-acetylglucosaminide + H2O - Trichoderma reesei PC-3-7 p-nitrophenol + beta-D-N-acetylglucosamine - ? 374229 3.2.1.165 shrimp shell chitosan + H2O more than 90% degree of acetylation, 57% activity compared to crab shell chitosan with more than 90% degree of acetylation Aspergillus fumigatus D-glucosamine + ? - ? 448113 3.2.1.165 soluble chitosan + H2O deacetylation degree 80-85%, 100% activity Penicillium sp. IB-37-2A D-glucosamine + ? - ? 448121