3.2.1.149 (R)-1-phenylethyl beta-D-glucopyranoside + H2O LC-MS analysis and quantitative GC-MS analysis of substrate and product Camellia sinensis (R)-1-phenylethanol + beta-D-glucopyranose - ? 431257 3.2.1.149 (R)-1-phenylethyl beta-primeveroside + H2O LC-MS analysis and quantitative GC-MS analysis of substrate and product Camellia sinensis (R)-1-phenylethanol + beta-primeverose - ? 431258 3.2.1.149 (R)-linalyl beta-vicianoside + H2O - Camellia sinensis linalol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside - ? 189650 3.2.1.149 (S)-1-phenylethyl beta-D-glucopyranoside + H2O LC-MS analysis and quantitative GC-MS analysis of substrate and product Camellia sinensis (S)-1-phenylethanol + beta-D-glucopyranose - ? 431260 3.2.1.149 (S)-1-phenylethyl beta-primeveroside + H2O LC-MS analysis and quantitative GC-MS analysis of substrate and product Camellia sinensis (S)-1-phenylethanol + beta-primeverose - ? 431261 3.2.1.149 (Z)-3-hexenyl beta-primeveroside + H2O pH 5.5, 40°C, 0.1% hydrolysis Penicillium multicolor (Z)-3-hexenol + primeverose - ? 380824 3.2.1.149 (Z)-3-hexenyl beta-primeveroside + H2O pH 5.5, 40°C, 0.1% hydrolysis Penicillium multicolor IAM7153 (Z)-3-hexenol + primeverose - ? 380824 3.2.1.149 2-phenylethyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O i.e. 2-phenylethyl beta-D-primeveroside Camellia sinensis 2-phenylethanol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose - ? 189659 3.2.1.149 2-phenylethyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O pH 6.0, 37°C, 0.05% activity Camellia sinensis 2-phenylethyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranose + 2-phenylethanol - ? 381042 3.2.1.149 2-phenylethyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O pH 6.0, 37°C, 0.60% activity Camellia sinensis beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranose + 2-phenylethanol - ? 381044 3.2.1.149 2-phenylethyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O trivial name beta-primeveroside Camellia sinensis 2-phenylethanol + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose - ? 370785 3.2.1.149 2-phenylethyl beta-acuminoside + H2O i.e. 2-phenylethyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside Camellia sinensis 2-phenylethanol + 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside - ? 189661 3.2.1.149 2-phenylethyl beta-D-glucopyranoside + H2O - Camellia sinensis 2-phenylethanol + D-glucopyranose - ? 189653 3.2.1.149 2-phenylethyl beta-D-glucopyranoside + H2O low activity Camellia sinensis 2-phenylethanol + beta-D-glucopyranose - ? 431331 3.2.1.149 2-phenylethyl beta-gentiobioside + H2O pH 6.0, 37°C, 0.34% activity Camellia sinensis gentiobiose + 2-phenylethanol - ? 381045 3.2.1.149 2-phenylethyl beta-primeveroside + H2O pH 5.5, 40°C, 0.1% hydrolysis Penicillium multicolor 2-phenylethanol + primeverose - ? 381046 3.2.1.149 2-phenylethyl beta-primeveroside + H2O pH 5.5, 40°C, 0.1% hydrolysis Penicillium multicolor IAM7153 2-phenylethanol + primeverose - ? 381046 3.2.1.149 2-phenylethyl beta-primeveroside + H2O pH 6.0, 37°C Camellia sinensis primeverose + 2-phenylethanol - ? 381047 3.2.1.149 2-phenylethyl beta-primeveroside + H2O pH 6.0, 37°C, 100% activity Camellia sinensis primeverose + 2-phenylethanol - ? 381047 3.2.1.149 2-phenylethyl beta-primeveroside + H2O - Camellia sinensis 2-phenylethanol + beta-primeverose - ? 431332 3.2.1.149 2-phenylethyl beta-vicianoside + H2O - Camellia sinensis 2-phenylethanol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside - ? 189651 3.2.1.149 2-phenylethyl beta-vicianoside + H2O pH 6.0, 37°C, 2.97% activity Camellia sinensis vicianose + 2-phenylethanol - ? 381048 3.2.1.149 2-phenylethyl-6-O-alpha-D-arabinosyl-beta-D-glucopyranoside + H2O trivial name beta-vicianoside, 3% of activity with 2-phenyl-beta-primeveroside Camellia sinensis 2-phenylethanol + 6-O-alpha-D-arabinosyl-beta-D-glucopyranoside - ? 371761 3.2.1.149 2-phenylethyl-6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O trivial name beta-vicianoside, 0.8% of activity with 2-phenyl-beta-primeveroside Camellia sinensis 2-phenylethanol + 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside - ? 371762 3.2.1.149 2-phenylethyl-6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside + H2O trivial name beta-vicianoside, 0.25% of activity with 2-phenyl-beta-primeveroside Camellia sinensis 2-phenylethanol + 6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside - ? 371763 3.2.1.149 4-nitrophenyl beta-D-glucopyranoside + H2O - Camellia sinensis 4-nitrophenol + D-glucopyranose - ? 94269 3.2.1.149 4-nitrophenyl beta-glucopyranoside + H2O pH 5.5, 40°C, 15% hydrolysis Penicillium multicolor 4-nitrophenol + D-glucopyranose - ? 382468 3.2.1.149 4-nitrophenyl beta-primeveroside + H2O i.e. 4-nitrophenyl-6-O-beta-D-xylopyranosyl-beta-D-glucopyranose Camellia sinensis 4-nitrophenol + primeverose - ? 189655 3.2.1.149 4-nitrophenyl-beta-primeveroside + H2O - Camellia sinensis 4-nitrophenol + beta-primeverose - ? 449351 3.2.1.149 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside + H2O hydrolyzed with lower activity Camellia sinensis alpha-L-arabinopyranose + beta-D-glucopyranose - ? 391057 3.2.1.149 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O hydrolyzed with lower activity Camellia sinensis beta-D-apiofuranose + beta-D-glucopyranose - ? 391059 3.2.1.149 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O hydrolyzed with highest activity Camellia sinensis beta-D-xylopyranose + beta-D-glucopyranose - ? 391061 3.2.1.149 6-O-beta-L-arabinopyranosyl-beta-D-glucopyranoside + H2O hydrolyzed with lower activity Camellia sinensis beta-D-arabinopyranose + beta-D-glucopyranose - ? 391062 3.2.1.149 a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O - Ipomoea batatas 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol - ? 430840 3.2.1.149 a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O - Camellia sinensis 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol - ? 430840 3.2.1.149 a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O the enzyme hydrolyzes beta-primeveroside at the beta-glycosidic bond of primeverose to aglycone, and releases aromatic alcoholic volatiles of aglycones Camellia sinensis 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol - ? 430840 3.2.1.149 a beta-primeveroside + H2O the enzyme shows broad substrate specificity with respect to the disaccharide glycon moiety (subsite-2), Thr271 and Thr415 play important roles in subsite-2 of the enzyme Camellia sinensis ? + primeverose - ? 391500 3.2.1.149 benzyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O i.e. 2-benzyl beta-D-primeveroside Camellia sinensis benzyl alcohol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose - ? 189660 3.2.1.149 benzyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O - Camellia sinensis benzyl alcohol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside - ? 189654 3.2.1.149 benzyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O - Camellia sinensis benzyl alcohol + beta-D-apiofuranosyl-(1->6)-D-glucose - ? 423059 3.2.1.149 benzyl beta-primeveroside + H2O pH 5.5, 40°C, 0.1% hydrolysis Penicillium multicolor phenylmethanol + primeverose - ? 381551 3.2.1.149 benzyl beta-primeveroside + H2O pH 5.5, 40°C, 0.1% hydrolysis Penicillium multicolor IAM7153 phenylmethanol + primeverose - ? 381551 3.2.1.149 cellobiose + H2O 37°C, 0.6% hydrolysis Aspergillus fumigatus 2 D-glucose - ? 94496 3.2.1.149 cellobiose + H2O 37°C, 0.6% hydrolysis Aspergillus fumigatus AP-20 2 D-glucose - ? 94496 3.2.1.149 eugenyl beta-primeveroside + H2O i.e. eugenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose Camellia sinensis eugenol + primeverose - ? 189657 3.2.1.149 eugenyl beta-primeveroside + H2O 37°C, 1.8% hydrolysis Aspergillus fumigatus eugenol + primeverose - ? 189657 3.2.1.149 eugenyl beta-primeveroside + H2O pH 5.5, 40°C, 2.5% hydrolysis Penicillium multicolor eugenol + primeverose - ? 189657 3.2.1.149 eugenyl beta-primeveroside + H2O 37°C, 1.8% hydrolysis Aspergillus fumigatus AP-20 eugenol + primeverose - ? 189657 3.2.1.149 eugenyl beta-primeveroside + H2O pH 5.5, 40°C, 2.5% hydrolysis Penicillium multicolor IAM7153 eugenol + primeverose - ? 189657 3.2.1.149 furcatin + H2O pH 5.5, 40°C, 1.0% hydrolysis Penicillium multicolor 4-allyl-phenol + ? - ? 381886 3.2.1.149 furcatin + H2O pH 5.5, 40°C, 1.0% hydrolysis Penicillium multicolor IAM7153 4-allyl-phenol + ? - ? 381886 3.2.1.149 gentiobiose + H2O 37°C, 5.1% hydrolysis Aspergillus fumigatus D-glucose - ? 94486 3.2.1.149 gentiobiose + H2O 37°C, 5.1% hydrolysis Aspergillus fumigatus AP-20 D-glucose - ? 94486 3.2.1.149 geranyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O i.e. geranyl beta-D-primeveroside Camellia sinensis geraniol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose - ? 189658 3.2.1.149 geranyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O - Camellia sinensis geraniol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside - ? 189652 3.2.1.149 geranyl beta-primeveroside + H2O pH 5.5, 40°C, 0.1% hydrolysis Penicillium multicolor geraniol + primeverose - ? 381946 3.2.1.149 laminaribiose + H2O 37°C, 1.6% hydrolysis Aspergillus fumigatus D-glucose - ? 94485 3.2.1.149 laminaribiose + H2O 37°C, 1.6% hydrolysis Aspergillus fumigatus AP-20 D-glucose - ? 94485 3.2.1.149 linalyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O - Camellia sinensis linalool + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose - ? 189649 3.2.1.149 linalyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O - Camellia sinensis linalol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose - ? 370015 3.2.1.149 additional information no activity with p-nitrophenol beta-D-xyloside and 2-phenylethyl beta-D-glucoside Camellia sinensis ? - ? 89 3.2.1.149 additional information does not act on 2-phenylethyl beta-D-glucopyranoside Camellia sinensis ? - ? 89 3.2.1.149 additional information the enzyme only accepts primeverose as the glycone substrate, but broadly accepts various aglycones, including 2-phenylethanol, benzyl alcohol, linalool, and geraniol. Compared with aglycone-specific beta-glucosidases of the glycoside hydrolase family 1, beta-primeverosidase lacks the Trp crucial for aglycone recognition, and the resultant large cavity accepts aglycone and 6-O-beta-D-xylopyranosyl together. The enzyme barely hydrolyzes other beta-glycosides, in which the beta-1,6-Xyl group is absent or substituted by other sugars, such as monosaccharide glycosides (beta-D-glucopyranoside and beta-D-xylopyranoside, etc.) or other disaccharide glycosides, beta-vicianosides (6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside), beta-gentiobiosides (6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside), and beta-acuminosides (6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside) Camellia sinensis ? - ? 89 3.2.1.149 additional information the diglycoside-specific enzyme beta-primeverosidase catalyses the hydrolysis of primeveroside into primeverose (a disaccharide of Xylbeta1-6Glc) and volatile aglycons Ipomoea batatas ? - ? 89 3.2.1.149 p-nitrophenol beta-D-glucopyranoside + H2O pH 6.0, 37°C, 0.23% activity Camellia sinensis D-glucose + p-nitrophenol - ? 382456 3.2.1.149 p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O trivial name beta-primeveroside Camellia sinensis p-nitrophenol + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose - ? 374180 3.2.1.149 p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O 37°C, 100% hydrolysis Aspergillus fumigatus primeverose + p-nitrophenol - ? 382457 3.2.1.149 p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O 37°C, 100% hydrolysis Aspergillus fumigatus AP-20 primeverose + p-nitrophenol - ? 382457 3.2.1.149 p-nitrophenyl beta-D-glucoside + H2O pH 6.0, 37°C, 0.5% activity (purified from tea), 0.3% activity (recombinant enzyme) Camellia sinensis p-nitrophenol + D-glucose - ? 382464 3.2.1.149 p-nitrophenyl beta-gentiobioside + H2O pH 5.5, 40°C, 2.5% hydrolysis Penicillium multicolor p-nitrophenol + D-gentiobiose - ? 382466 3.2.1.149 p-nitrophenyl beta-gentiobioside + H2O 37°C, 41% hydrolysis Aspergillus fumigatus p-nitrophenol + gentiobiose - ? 382467 3.2.1.149 p-nitrophenyl beta-primeveroside + H2O pH 5.5, 40°C, 100% hydrolysis Penicillium multicolor primeverose + p-nitrophenol - ? 382469 3.2.1.149 p-nitrophenyl beta-primeveroside + H2O pH 6.0, 37°C Camellia sinensis primeverose + p-nitrophenol - ? 382469 3.2.1.149 p-nitrophenyl beta-primeveroside + H2O pH 6.0, 37°C, 48% activity (purified from tea), 62% activity (recombinant enzyme) Camellia sinensis primeverose + p-nitrophenol - ? 382469 3.2.1.149 p-nitrophenyl beta-primeveroside + H2O pH 6.0, 37°C, 54.4% activity Camellia sinensis primeverose + p-nitrophenol - ? 382469 3.2.1.149 p-nitrophenyl-beta-D-glucopyranoside + H2O - Camellia sinensis 4-nitrophenol + beta-D-glucose - ? 259830 3.2.1.149 p-nitrophenyl-beta-D-glucopyranoside + H2O - Camellia sinensis p-nitrophenol + beta-D-glucose - ? 374213 3.2.1.149 primeverose + H2O 37°C, 0.2% hydrolysis Aspergillus fumigatus D-xylose + D-glucose - ? 382654 3.2.1.149 sophorose + H2O 37°C, 0.5% hydrolysis Aspergillus fumigatus 2 D-glucose - ? 382823