3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O - Nostoc punctiforme (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose - ? 393520 3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O yield of trehalose production is relatively low but may be increased in the presence of alpha-amylase Metallosphaera hakonensis (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose - ? 393520 3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O enzyme is up-regulated under NaCl stress, starting at about 36 hours of exposure, acts together with maltooligosyltrehalose synthase Anabaena sp. (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose - r 393520 3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O hydrolysis to disaccharides after 24 h Metallosphaera hakonensis (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose - ? 393520 3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O with isoamylase and maltooligosyltrehalose synthase Saccharolobus solfataricus (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose - ? 393520 3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O - Nostoc punctiforme IAM M-15 (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose - ? 393520 3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O yield of trehalose production is relatively low but may be increased in the presence of alpha-amylase Metallosphaera hakonensis JCM 8857 (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose - ? 393520 3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O hydrolysis to disaccharides after 24 h Metallosphaera hakonensis JCM 8857 (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose - ? 393520 3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O hydrolysis to disaccharides after 24 h Metallosphaera hakonensis JMC8857 (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose - ? 393520 3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O enzyme is up-regulated under NaCl stress, starting at about 36 hours of exposure, acts together with maltooligosyltrehalose synthase Anabaena sp. 7120 (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose - r 393520 3.2.1.141 glucosyl trehalose + H2O - Sulfolobus acidocaldarius glucose + trehalose - ? 13787 3.2.1.141 maltoheptaose + H2O - Saccharolobus solfataricus ? - ? 94186 3.2.1.141 maltoheptaosyltrehalose + H2O - Corynebacterium glutamicum maltoheptaose + alpha,alpha'-trehalose - ? 392531 3.2.1.141 maltohexaose + H2O - Saccharolobus solfataricus ? - ? 94185 3.2.1.141 maltohexaose + H2O for glucose formation, maltohexaose exhibits the highest formation rate Saccharolobus solfataricus ? - ? 94185 3.2.1.141 maltooligosacccharide + H2O - Saccharolobus solfataricus D-glucose - ? 405770 3.2.1.141 maltooligosyltrehalose + H2O - Nostoc flagelliforme maltooligosaccharide + alpha,alpha'-trehalose - ? 405771 3.2.1.141 maltooligosyltrehalose + H2O alpha-1,4-glucosidic and alpha-1,1-linked terminal dissaccharides of maltooligosyltrehalose Saccharolobus solfataricus maltooligosaccharide + alpha,alpha'-trehalose trehalose ? 405771 3.2.1.141 maltooligosyltrehalose + H2O - Saccharolobus solfataricus trehalose + ? - ? 426632 3.2.1.141 maltooligosyltrehalose + H2O - Saccharolobus solfataricus KM1 trehalose + ? - ? 426632 3.2.1.141 maltopentaose + H2O - Saccharolobus solfataricus ? - ? 94184 3.2.1.141 maltopentaosyltrehalose + H2O - Sulfolobus acidocaldarius maltopentaose + alpha,alpha'-trehalose - ? 392540 3.2.1.141 maltopentaosyltrehalose + H2O - Arthrobacter sp. maltopentaose + alpha,alpha'-trehalose - ? 392540 3.2.1.141 maltopentaosyltrehalose + H2O - Saccharolobus solfataricus maltopentaose + alpha,alpha'-trehalose - ? 392540 3.2.1.141 maltopentaosyltrehalose + H2O trehalose formation rate increases as the degree of polymerization value of the substrate increases. maltopentaosyl trehalose is the most preferred substrate for trehalose formation Saccharolobus solfataricus maltopentaose + alpha,alpha'-trehalose - ? 392540 3.2.1.141 maltopentaosyltrehalose + H2O - Arthrobacter sp. O36 maltopentaose + alpha,alpha'-trehalose - ? 392540 3.2.1.141 maltosylpentaosyltrehalose + H2O 93.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus trehalose + maltopentaose - ? 423820 3.2.1.141 maltosylpentaosyltrehalose + H2O 93.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus KM1 trehalose + maltopentaose - ? 423820 3.2.1.141 maltosyltetraosyltrehalose + H2O hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus trehalose + maltotetraose - ? 423821 3.2.1.141 maltosyltetraosyltrehalose + H2O hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus KM1 trehalose + maltotetraose - ? 423821 3.2.1.141 maltosyltrehalose + H2O - Sulfolobus acidocaldarius maltose + alpha,alpha'-trehalose - ? 13788 3.2.1.141 maltosyltrehalose + H2O - Arthrobacter sp. maltose + alpha,alpha'-trehalose - ? 13788 3.2.1.141 maltosyltrehalose + H2O - Saccharolobus solfataricus maltose + alpha,alpha'-trehalose - ? 13788 3.2.1.141 maltosyltrehalose + H2O - Arthrobacter sp. O36 maltose + alpha,alpha'-trehalose - ? 13788 3.2.1.141 maltosyltrehalose + H2O 14.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus trehalose + maltose - ? 423822 3.2.1.141 maltosyltrehalose + H2O 14.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus KM1 trehalose + maltose - ? 423822 3.2.1.141 maltosyltriosyltrehalose + H2O 91.6% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus trehalose + maltotriose - ? 423823 3.2.1.141 maltosyltriosyltrehalose + H2O 91.6% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose Saccharolobus solfataricus KM1 trehalose + maltotriose - ? 423823 3.2.1.141 maltotetraose + H2O - Saccharolobus solfataricus ? - ? 94183 3.2.1.141 maltotetraosyltrehalose + H2O - Sulfolobus acidocaldarius maltotetraose + alpha,alpha'-trehalose - ? 13790 3.2.1.141 maltotetraosyltrehalose + H2O - Arthrobacter sp. maltotetraose + alpha,alpha'-trehalose - ? 13790 3.2.1.141 maltotetraosyltrehalose + H2O - Saccharolobus solfataricus maltotetraose + alpha,alpha'-trehalose - ? 13790 3.2.1.141 maltotetraosyltrehalose + H2O - Arthrobacter sp. O36 maltotetraose + alpha,alpha'-trehalose - ? 13790 3.2.1.141 maltotriosyltrehalose + H2O - Sulfolobus acidocaldarius maltotriose + alpha,alpha'-trehalose - ? 13789 3.2.1.141 maltotriosyltrehalose + H2O - Arthrobacter sp. maltotriose + alpha,alpha'-trehalose - ? 13789 3.2.1.141 maltotriosyltrehalose + H2O - Saccharolobus solfataricus maltotriose + alpha,alpha'-trehalose - ? 13789 3.2.1.141 maltotriosyltrehalose + H2O - Metallosphaera hakonensis maltotriose + alpha,alpha'-trehalose - ? 13789 3.2.1.141 maltotriosyltrehalose + H2O sequential hydrolysis by the bifunctional fusion enzyme of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH), synthase converts alpha-1,4-glycosidic linkage into alpha-1,1 linkage to produce maltotriosyltrehalose, which is the substrate for the MTH to produce trehalose Metallosphaera hakonensis maltotriose + alpha,alpha'-trehalose - ? 13789 3.2.1.141 maltotriosyltrehalose + H2O - Metallosphaera hakonensis JCM 8857 maltotriose + alpha,alpha'-trehalose - ? 13789 3.2.1.141 maltotriosyltrehalose + H2O sequential hydrolysis by the bifunctional fusion enzyme of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH), synthase converts alpha-1,4-glycosidic linkage into alpha-1,1 linkage to produce maltotriosyltrehalose, which is the substrate for the MTH to produce trehalose Metallosphaera hakonensis JCM 8857 maltotriose + alpha,alpha'-trehalose - ? 13789 3.2.1.141 maltotriosyltrehalose + H2O sequential hydrolysis by the bifunctional fusion enzyme of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH), synthase converts alpha-1,4-glycosidic linkage into alpha-1,1 linkage to produce maltotriosyltrehalose, which is the substrate for the MTH to produce trehalose Metallosphaera hakonensis JMC8857 maltotriose + alpha,alpha'-trehalose - ? 13789 3.2.1.141 maltotriosyltrehalose + H2O - Arthrobacter sp. O36 maltotriose + alpha,alpha'-trehalose - ? 13789 3.2.1.141 maltotriosyltrehalose + H2O - Brevibacterium helvolum trehalose + maltotriose - ? 385119 3.2.1.141 additional information the enzyme is involved in the biosynthesis of trehalose from maltooligosaccharides Arthrobacter sp. ? - ? 89 3.2.1.141 additional information increase in trehalose level during NaCl stress is the result of overexpression of the trehalose-forming enzymes malooligosyltrehalose synthase (EC 5.4.99.15) and maltooligosyltrehalose trehalohydrolase Nostoc sp. PCC 7120 = FACHB-418 ? - ? 89 3.2.1.141 additional information MTHase catalyzes the release of trehalose by cleaving the alpha-1,4-glucosidic linkage next to the alpha-1,1-linked terminal disaccharide of maltooligosyltrehalose, D255, E286, and D380 are essential catalytic residues, while residues A259, Y328, F355, and R356 are related to enzyme selectivity, mutational analysis, overview Saccharolobus solfataricus ? - ? 89 3.2.1.141 additional information hydrolysis of G3T-G5T and G5-G7. Production of trehalose from starch together with isoamylase and maltooligosyltrehalose synthase, overview Saccharolobus solfataricus ? - ? 89 3.2.1.141 additional information the crystal structure reveals a scheme for substrate recognition that involves two unique interactions: stacking of Tyr325 with the terminal glucose ring of the trehalose moiety and perpendicularly placement of Trp215 to the pyranose rings at the subsites -1 and +1 glucose Saccharolobus solfataricus ? - ? 89 3.2.1.141 additional information the enzyme releases trehalose from maltooligosyltrehalose Brevibacterium helvolum ? - ? 89 3.2.1.141 additional information maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose Sulfolobus acidocaldarius ? - ? 89 3.2.1.141 additional information maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose. The recombinant MTHase can catalyze the production of trehalose from starch at high yield when coupled with the maltooligosyl trehalose synthase from the same strain Sulfolobus acidocaldarius ? - ? 89 3.2.1.141 additional information substrate potato starch in combined reaction with trehalose-producing (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase, MTSase (EC 5.4.99.15), recombinant enzyme Saccharolobus solfataricus ? - ? 89 3.2.1.141 additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii ? - ? 89 3.2.1.141 additional information MTHase catalyzes the release of trehalose by cleaving the alpha-1,4-glucosidic linkage next to the alpha-1,1-linked terminal disaccharide of maltooligosyltrehalose, D255, E286, and D380 are essential catalytic residues, while residues A259, Y328, F355, and R356 are related to enzyme selectivity, mutational analysis, overview Saccharolobus solfataricus P2 ? - ? 89 3.2.1.141 additional information hydrolysis of G3T-G5T and G5-G7. Production of trehalose from starch together with isoamylase and maltooligosyltrehalose synthase, overview Saccharolobus solfataricus P2 ? - ? 89 3.2.1.141 additional information the crystal structure reveals a scheme for substrate recognition that involves two unique interactions: stacking of Tyr325 with the terminal glucose ring of the trehalose moiety and perpendicularly placement of Trp215 to the pyranose rings at the subsites -1 and +1 glucose Saccharolobus solfataricus KM1 ? - ? 89 3.2.1.141 additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii 7 ? - ? 89 3.2.1.141 additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii DSM 16993 ? - ? 89 3.2.1.141 additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii JCM 10545 ? - ? 89 3.2.1.141 additional information maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose Sulfolobus acidocaldarius MT4 ? - ? 89 3.2.1.141 additional information maltooligosyl trehalose trehalohydrolase (MTHase) catalyzes the release of trehalose by splitting the alpha-1,4-glucosidic linkage adjacent to the alpha-1,1-glucosidic linkage of maltooligosyl trehalose. The recombinant MTHase can catalyze the production of trehalose from starch at high yield when coupled with the maltooligosyl trehalose synthase from the same strain Sulfolobus acidocaldarius MT4 ? - ? 89 3.2.1.141 additional information substrate potato starch in combined reaction with trehalose-producing (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase, MTSase (EC 5.4.99.15), recombinant enzyme Saccharolobus solfataricus ATCC 33909 ? - ? 89 3.2.1.141 additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii NBRC 100140 ? - ? 89