3.1.4.37 1,N6-etheno-2-azaadenosine-2',3'-cyclic monophosphate + H2O - Rattus norvegicus etheno-2-azaadenosine-2'-monophosphate - ? 93690 3.1.4.37 1,N6-ethenoadenosine-2',3'-cyclic monophosphate + H2O - Rattus norvegicus ethenoadenosine-2'-monophosphate - ? 93691 3.1.4.37 2', 3'-cyclic ribonucleotide - Mammalia ribonucleoside 2'-phosphate - ? 93698 3.1.4.37 2',3'-cAMP + H2O - Gallus gallus 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Cavia porcellus 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Haemophilus influenzae 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Mus musculus 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Homo sapiens 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Rattus norvegicus 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Sus scrofa 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Bos taurus 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Triticum aestivum 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Oryctolagus cuniculus 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Ovis aries 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Spinacia oleracea 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Canis lupus familiaris 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Xenopus laevis 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Carassius auratus 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Chondrichthyes 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Artemia sp. 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Danio rerio 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O the protein consists of two domains: an uncharacterized N-terminal domain with little homology to other proteins, and a C-terminal phosphodiesterase domain. C-terminal tail (22 residues) has no significant effect on the catalytic activity, neither do the first 24 N-terminal residues of the full-length protein. Both the N- and C-terminal domain of recombinant CNPase are folded entities: N-terminal domain has more beta-sheet structure and less alpha-helices than the C-terminal domain Mus musculus 2'-AMP - ? 93696 3.1.4.37 2',3'-cAMP + H2O - Escherichia coli 2'-AMP + 3'-AMP - ? 371554 3.1.4.37 2',3'-cAMP + H2O the exclusive formation of 3'-AMP is due to the P-O2' bond having lower activation energy and is not the result of steric exclusion at enzyme active site, kinetic evidence that hydrolysis of 2',3'-cAMP into 3'-AMP is nonspecific. Modeling of 2',3'-cyclic nucleotide into the active site of a EAL domain PDE, overview Rattus norvegicus 3'-AMP + ? - ? 416091 3.1.4.37 2',3'-cCMP + H2O - Cavia porcellus 2'-CMP - ? 93692 3.1.4.37 2',3'-cCMP + H2O - Homo sapiens 2'-CMP - ? 93692 3.1.4.37 2',3'-cCMP + H2O - Rattus norvegicus 2'-CMP - ? 93692 3.1.4.37 2',3'-cCMP + H2O - Sus scrofa 2'-CMP - ? 93692 3.1.4.37 2',3'-cCMP + H2O - Bos taurus 2'-CMP - ? 93692 3.1.4.37 2',3'-cCMP + H2O - Triticum aestivum 2'-CMP - ? 93692 3.1.4.37 2',3'-cCMP + H2O - Spinacia oleracea 2'-CMP - ? 93692 3.1.4.37 2',3'-cCMP + H2O - Artemia sp. 2'-CMP - ? 93692 3.1.4.37 2',3'-cCMP + H2O - Mus musculus 2'-CMP - ? 93692 3.1.4.37 2',3'-cGMP + H2O - Mus musculus 2'-GMP - ? 93695 3.1.4.37 2',3'-cGMP + H2O - Sus scrofa 2'-GMP - ? 93695 3.1.4.37 2',3'-cGMP + H2O - Bos taurus 2'-GMP - ? 93695 3.1.4.37 2',3'-cGMP + H2O - Triticum aestivum 2'-GMP - ? 93695 3.1.4.37 2',3'-cGMP + H2O - Spinacia oleracea 2'-GMP - ? 93695 3.1.4.37 2',3'-cGMP + H2O - Artemia sp. 2'-GMP - ? 93695 3.1.4.37 2',3'-cGMP + H2O - Escherichia coli 2'-GMP + 3'-GMP - ? 371555 3.1.4.37 2',3'-cNADP+ + H2O - Rattus norvegicus 2'-NADP+ - ? 93693 3.1.4.37 2',3'-cNADP+ + H2O - Bos taurus 2'-NADP+ - ? 93693 3.1.4.37 2',3'-cNADP+ + H2O - Rattus norvegicus NADP+ - ? 364660 3.1.4.37 2',3'-cNADP+ + H2O - Mus musculus NADP+ - ? 364660 3.1.4.37 2',3'-cNADP+ + H2O - Mus musculus ? - ? 429653 3.1.4.37 2',3'-cUMP + H2O - Sus scrofa 2'-UMP - ? 93694 3.1.4.37 2',3'-cUMP + H2O - Bos taurus 2'-UMP - ? 93694 3.1.4.37 2',3'-cUMP + H2O - Triticum aestivum 2'-UMP - ? 93694 3.1.4.37 2',3'-cUMP + H2O - Artemia sp. 2'-UMP - ? 93694 3.1.4.37 2',3'-cyclic AMP + H2O - Mus musculus 2'-AMP - ? 429136 3.1.4.37 2',3'-cyclic AMP + H2O - Homo sapiens 2'-AMP - ? 429136 3.1.4.37 2',3'-cyclic AMP + H2O - Rattus norvegicus 2'-AMP - ? 429136 3.1.4.37 2',3'-cyclic AMP + H2O - Oryctolagus cuniculus 2'-AMP - ? 429136 3.1.4.37 2',3'-cyclic AMP + H2O product binding structure, overview Mus musculus 2'-AMP - ? 429136 3.1.4.37 2',3'-cyclic AMP + H2O substrate and product enzyme binding structures, overview Mus musculus 2'-AMP - ? 429136 3.1.4.37 2',3'-cyclic NADP+ + H2O - Rattus norvegicus NADP+ - ? 364659 3.1.4.37 2',3'-cyclic NADP+ + H2O - Bos taurus NADP+ - ? 364659 3.1.4.37 2',3'-cyclic-2-aza-e-AMP + H2O - Bos taurus 2'-2-aza-e-AMP - ? 93687 3.1.4.37 2',3'-cyclic-e-AMP + H2O - Bos taurus 2'-e-AMP - ? 93689 3.1.4.37 2'-AMP + H2O - Escherichia coli adenosine + phosphate - ? 64619 3.1.4.37 3'-AMP + H2O - Bacillus subtilis ? - ? 93697 3.1.4.37 adenosine 2,3-cyclic phosphorothioate + H2O the Sp epimer of 2',3'-cAMPS is a functional substrate for the enzyme, while the Rp epimer is not Mus musculus ? - ? 429873 3.1.4.37 ADP + H2O - Escherichia coli AMP + phosphate - ? 93189 3.1.4.37 ATP + H2O - Escherichia coli ADP + phosphate - ? 92967 3.1.4.37 bis(p-nitrophenyl)phosphate + H2O non-specific substrate Rattus norvegicus ? - ? 73716 3.1.4.37 bis-p nitrophenyl phosphate + H2O - Bacillus subtilis ? - ? 93699 3.1.4.37 bis-p-nitrophenyl phosphate + H2O - Escherichia coli ? - ? 73498 3.1.4.37 CDP + H2O - Escherichia coli CMP + phosphate - ? 93276 3.1.4.37 CTP + H2O - Escherichia coli CDP + phosphate - ? 93269 3.1.4.37 cyclic 2',3'-AMP + H2O - Rattus norvegicus 2'-AMP - ? 364656 3.1.4.37 cyclic 2',3'-AMP + H2O - Acetivibrio thermocellus 2'-AMP - ? 364656 3.1.4.37 cyclic 2',3'-AMP + H2O - Homo sapiens 2'-AMP - ? 364656 3.1.4.37 cyclic 2',3'-AMP + H2O best substrate Escherichia coli 2'-AMP - ? 364656 3.1.4.37 cyclic 2',3'-AMP + H2O natural substrate, the 2',3'-cyclic phosphodiesterase activity of the C-terminal HD domain of tRNA nucleotidyltransferase, EC 2.7.7.25, is involved in the repair of the 3’-CCA end of tRNA Escherichia coli 2'-AMP - ? 364656 3.1.4.37 cyclic 2',3'-CMP + H2O low activity Escherichia coli 2'-CMP - ? 364658 3.1.4.37 cyclic 2',3'-GMP + H2O high activity Escherichia coli 2'-GMP - ? 364657 3.1.4.37 diphosphate + H2O - Escherichia coli 2 phosphate - ? 73540 3.1.4.37 additional information - Gallus gallus ? - ? 89 3.1.4.37 additional information - Cavia porcellus ? - ? 89 3.1.4.37 additional information - Haemophilus influenzae ? - ? 89 3.1.4.37 additional information - Bacillus subtilis ? - ? 89 3.1.4.37 additional information - Mus musculus ? - ? 89 3.1.4.37 additional information - Homo sapiens ? - ? 89 3.1.4.37 additional information - Rattus norvegicus ? - ? 89 3.1.4.37 additional information - Sus scrofa ? - ? 89 3.1.4.37 additional information - Bos taurus ? - ? 89 3.1.4.37 additional information - Oryctolagus cuniculus ? - ? 89 3.1.4.37 additional information - Ovis aries ? - ? 89 3.1.4.37 additional information - Spinacia oleracea ? - ? 89 3.1.4.37 additional information - Brassica napus ? - ? 89 3.1.4.37 additional information - Canis lupus familiaris ? - ? 89 3.1.4.37 additional information - Xenopus laevis ? - ? 89 3.1.4.37 additional information - Chondrichthyes ? - ? 89 3.1.4.37 additional information - Ophiophagus hannah ? - ? 89 3.1.4.37 additional information not: 2',3'-cyclic esters in cyclic phosphate-terminated oligoribonucleotides or in nucleoside 5'-phosphate, 2',3'-cyclic phosphate Triticum aestivum ? - ? 89 3.1.4.37 additional information three enzyme forms Rattus norvegicus ? - ? 89 3.1.4.37 additional information not: 3',5'-cyclic nucleotides Gallus gallus ? - ? 89 3.1.4.37 additional information not: 3',5'-cyclic nucleotides Cavia porcellus ? - ? 89 3.1.4.37 additional information not: 3',5'-cyclic nucleotides Haemophilus influenzae ? - ? 89 3.1.4.37 additional information not: 3',5'-cyclic nucleotides Mus musculus ? - ? 89 3.1.4.37 additional information not: 3',5'-cyclic nucleotides Homo sapiens ? - ? 89 3.1.4.37 additional information not: 3',5'-cyclic nucleotides Rattus norvegicus ? - ? 89 3.1.4.37 additional information not: 3',5'-cyclic nucleotides Sus scrofa ? - ? 89 3.1.4.37 additional information not: 3',5'-cyclic nucleotides Bos taurus ? - ? 89 3.1.4.37 additional information not: 3',5'-cyclic nucleotides Oryctolagus cuniculus ? - ? 89 3.1.4.37 additional information not: 3',5'-cyclic nucleotides Ovis aries ? - ? 89 3.1.4.37 additional information not: 3',5'-cyclic nucleotides Canis lupus familiaris ? - ? 89 3.1.4.37 additional information not: 3',5'-cyclic nucleotides Xenopus laevis ? - ? 89 3.1.4.37 additional information not: 3',5'-cyclic nucleotides Chondrichthyes ? - ? 89 3.1.4.37 additional information not: nucleoside 3',5'-cyclic phosphate Escherichia coli ? - ? 89 3.1.4.37 additional information early stages of myelin formation Mus musculus ? - ? 89 3.1.4.37 additional information CNP acts as microtubule-associated protein in promoting microtubule assembly at low mole ratios, links tubulin to membranes and may regulate cytoplasmic microtubule distribution, membrane anchor for tubulin Rattus norvegicus ? - ? 89 3.1.4.37 additional information CNP is one of the earliest myelin-related proteins to be expressed in differentiated oligodendrocytes and Schwann cells, role in migration and/or expansion of membranes during myelination Rattus norvegicus ? - ? 89 3.1.4.37 additional information CNP performs an integral role in myelinogenesis, both isoforms likely play complementary roles in the onset of process outgrowth and ultimately myelination of axons Rattus norvegicus ? - ? 89 3.1.4.37 additional information early oligodendroglial marker, CNPase links myelin related proteins to the cytoskeleton also interacting with membrane lipids during extension and wrapping of the oligodendroglial process around the axon, in mature myelinated fiber the CNPase is absent from compact myelin sheath, being located only in the inner and outer loops and in paranodal loops Rattus norvegicus ? - ? 89 3.1.4.37 additional information myelin-specific protein, synthesized by oligodendrocytes, more abundant in the myelin of the CNS than in that of the peripheral nervous system, CNP is regulated by several protein kinases and may have a role in morphological changes of the cells by modulating the cytoskeleton Rattus norvegicus ? - ? 89 3.1.4.37 additional information non-compact myelin protein, may have a unique role in signaling pathways mediated by lipid-protein domains Rattus norvegicus ? - ? 89 3.1.4.37 additional information related to axonal ensheathment by myelinating cells Rattus norvegicus ? - ? 89 3.1.4.37 additional information the cAMP-mediated pathway is part of the molecular mechanisms regulating the expression of CNP1 in oligodendrocytes, roles of AP-2, AP-4 and nuclear factor-1 in the regulation of CNP1 expression Mus musculus ? - ? 89 3.1.4.37 additional information the cAMP-mediated pathway is part of the molecular mechanisms regulating the expression of CNP1 in oligodendrocytes, roles of AP-2, AP-4, nuclear factor-1 and protein kinase A in the regulation of CNP1 expression Rattus norvegicus ? - ? 89 3.1.4.37 additional information endogenous enzyme expression is augmented by juxtanodin transfection, an oligodendroglial protein that promotes cellular arborization. Juxtanodin also augments transport of enzyme to the process arbors of cultured primary oligodendrocyte precursors Rattus norvegicus ? - ? 89 3.1.4.37 additional information enzyme is involved in mediating process formation in oligodendrocytes Mus musculus ? - ? 89 3.1.4.37 additional information enzyme can perform the essential 3'-end-healing steps of tRNA splicing in yeast and complement growth of strains bearing lethal or temperature-sensitive mutations in the tRNA ligase 3'-end-healing domain Rattus norvegicus ? - ? 89 3.1.4.37 additional information enzyme binds purine nucleotide triphosphates with an affinity higher than that displayed for diphosphates, while the affinity for both purine monophosphates and pyrimidine nucleotides is negligible. Analysis of binding constants for GTP, GDP, GMP, ATP, ADP, CTP, CDP, UTP, UDP Rattus norvegicus ? - ? 89 3.1.4.37 additional information CNP is an RNA-binding protein, CNP associates with poly(A)+ mRNAs in vivo and suppresses translation in vitro in a dose-dependent manner, isoform CNP1 may regulate expression of mRNAs in oligodendrocytes of the central nervous system Rattus norvegicus ? - ? 89 3.1.4.37 additional information endogenous Nogo-A interacts with the endogenous CNP in vitro and in vivo Homo sapiens ? - ? 89 3.1.4.37 additional information substrate specificities and activities of RocR, DGC2, YybT, YtqI, 3DMA, and PaAcpH with 2',3'-cAMP, overview Rattus norvegicus ? - ? 89 3.1.4.37 NADP+ + H2O - Escherichia coli NAD+ + phosphate - ? 73730 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Gallus gallus nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Cavia porcellus nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Haemophilus influenzae nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Mus musculus nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Escherichia coli nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Homo sapiens nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Rattus norvegicus nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Sus scrofa nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Bos taurus nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Triticum aestivum nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Oryctolagus cuniculus nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Ovis aries nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Arabidopsis thaliana nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Canis lupus familiaris nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Xenopus laevis nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Chondrichthyes nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O activity resides in the C-terminus Rattus norvegicus nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O His-230 and His-309 of CNP1 play critical roles in enzyme catalysis, no essential role of cysteines, the catalytic domain forms a compact globular structure Rattus norvegicus nucleoside 2'-phosphate - ir 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O plays a role in cytoskeletal rearrangement, cell morphology and cell process outgrowth, increased expression of CNP is a marker for oligodendrocyte development Rattus norvegicus nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O dinucleotide binding mode, overview Mus musculus nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O - Rattus norvegicus Wistar nucleoside 2'-phosphate - ? 93688 3.1.4.37 nucleoside 2',3'-cyclic phosphates + H2O - Gallus gallus ? - ? 369742 3.1.4.37 nucleoside 2',3'-cyclic phosphates + H2O - Cavia porcellus ? - ? 369742 3.1.4.37 nucleoside 2',3'-cyclic phosphates + H2O - Haemophilus influenzae ? - ? 369742 3.1.4.37 nucleoside 2',3'-cyclic phosphates + H2O - Mus musculus ? - ? 369742 3.1.4.37 nucleoside 2',3'-cyclic phosphates + H2O - Homo sapiens ? - ? 369742 3.1.4.37 nucleoside 2',3'-cyclic phosphates + H2O - Rattus norvegicus ? - ? 369742 3.1.4.37 nucleoside 2',3'-cyclic phosphates + H2O - Sus scrofa ? - ? 369742 3.1.4.37 nucleoside 2',3'-cyclic phosphates + H2O - Bos taurus ? - ? 369742 3.1.4.37 nucleoside 2',3'-cyclic phosphates + H2O - Oryctolagus cuniculus ? - ? 369742 3.1.4.37 nucleoside 2',3'-cyclic phosphates + H2O - Ovis aries ? - ? 369742 3.1.4.37 nucleoside 2',3'-cyclic phosphates + H2O - Canis lupus familiaris ? - ? 369742 3.1.4.37 nucleoside 2',3'-cyclic phosphates + H2O - Xenopus laevis ? - ? 369742 3.1.4.37 nucleoside 2',3'-cyclic phosphates + H2O - Chondrichthyes ? - ? 369742 3.1.4.37 p-nitrophenyl phosphate + H2O - Escherichia coli p-nitrophenol + phosphate - ? 64623