3.1.4.17 2',3'-cAMP + H2O - Myxococcus xanthus ? - ? 93153 3.1.4.17 2',3'-cAMP + H2O - Mycobacterium tuberculosis ? - ? 93153 3.1.4.17 2',3'-cAMP + H2O - Arthrobacter sp. 5'-AMP - ? 429652 3.1.4.17 2',3'-cAMP + H2O - Arthrobacter sp. CGMCC 3584 5'-AMP - ? 429652 3.1.4.17 3',5'-cAMP + H2O - Cavia porcellus 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Drosophila melanogaster 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Mus musculus 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Homo sapiens 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Rattus norvegicus 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Bos taurus 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Oryctolagus cuniculus 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Neurospora crassa 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Pseudomonas aeruginosa 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Canis lupus familiaris 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Xenopus laevis 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Corynebacterium glutamicum 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Mycobacterium tuberculosis 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Trypanosoma cruzi 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Myxococcus xanthus 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Sinorhizobium fredii 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Anabaena sp. 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Saccharomyces cerevisiae 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Arthrobacter sp. 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Corynebacterium glutamicum ATCC 13032 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O - Arthrobacter sp. CGMCC 3584 5'-AMP - ? 93634 3.1.4.17 3',5'-cCMP + H2O - Neurospora crassa 5'-CMP - ? 93637 3.1.4.17 3',5'-cCMP + H2O - Canis lupus familiaris 5'-CMP - ? 93637 3.1.4.17 3',5'-cdAMP + H2O - Canis lupus familiaris 5'-dAMP - ? 93644 3.1.4.17 3',5'-cGMP + H2O - Cavia porcellus 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Drosophila melanogaster 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Mus musculus 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Homo sapiens 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Rattus norvegicus 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Bos taurus 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Oryctolagus cuniculus 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Neurospora crassa 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Pseudomonas aeruginosa 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Canis lupus familiaris 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Xenopus laevis 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Corynebacterium glutamicum 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Mycobacterium tuberculosis 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Trypanosoma cruzi 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Sinorhizobium fredii 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Anabaena sp. 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Saccharomyces cerevisiae 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Arthrobacter sp. 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Corynebacterium glutamicum ATCC 13032 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Arthrobacter sp. CGMCC 3584 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O - Chlamydomonas reinhardtii guanosine 5'-phosphate - ? 383021 3.1.4.17 5'-AMP + H2O - Myxococcus xanthus ? - ? 93414 3.1.4.17 5'-ATP + H2O - Myxococcus xanthus ? - ? 73513 3.1.4.17 5'-pApA + H2O - Thermotoga maritima 5'-AMP - ? 444803 3.1.4.17 5'-pApA + H2O - Thermotoga maritima DSM 3109 5'-AMP - ? 444803 3.1.4.17 5'-pApG + H2O - Thermotoga maritima 5'-AMP + 5'-GMP - ? 444804 3.1.4.17 5'-pApG + H2O - Thermotoga maritima DSM 3109 5'-AMP + 5'-GMP - ? 444804 3.1.4.17 5'-pGpG + H2O - Thermotoga maritima 5'-GMP - ? 444805 3.1.4.17 5'-pGpG + H2O - Thermotoga maritima DSM 3109 5'-GMP - ? 444805 3.1.4.17 adenosine 3',5'-cyclic phosphate + H2O - Homo sapiens adenosine 5'-phosphate - ? 394684 3.1.4.17 adenosine 3',5'-cyclic phosphate + H2O - Myxococcus xanthus adenosine 5'-phosphate - ? 394684 3.1.4.17 bis-(4-nitrophenyl) phosphate + H2O - Corynebacterium glutamicum 4-nitrophenyl phosphate + 4-nitrophenol - ? 447240 3.1.4.17 bis-(4-nitrophenyl) phosphate + H2O - Corynebacterium glutamicum ATCC 13032 4-nitrophenyl phosphate + 4-nitrophenol - ? 447240 3.1.4.17 cAMP + H2O - Rattus norvegicus AMP - ? 364641 3.1.4.17 cAMP + H2O - Bos taurus AMP - ? 364641 3.1.4.17 cAMP + H2O - Sinorhizobium fredii AMP - ? 364641 3.1.4.17 cAMP + H2O - Homo sapiens AMP - ? 364641 3.1.4.17 cAMP + H2O - Sinorhizobium fredii MAR-1 AMP - ? 364641 3.1.4.17 cGMP + H2O - Rattus norvegicus GMP - ? 364639 3.1.4.17 cGMP + H2O - Bos taurus GMP - ? 364639 3.1.4.17 cGMP + H2O - Sinorhizobium fredii GMP - ? 364639 3.1.4.17 cGMP + H2O - Homo sapiens GMP - ? 364639 3.1.4.17 cGMP + H2O - Sinorhizobium fredii MAR-1 GMP - ? 364639 3.1.4.17 guanosine 3',5'-cyclic phosphate + H2O - Homo sapiens guanosine 5'-phosphate - ? 364651 3.1.4.17 guanosine 3',5'-cyclic phosphate + H2O - Rattus norvegicus guanosine 5'-phosphate - ? 364651 3.1.4.17 guanosine 3',5'-cyclic phosphate + H2O - Myxococcus xanthus guanosine 5'-phosphate - ? 364651 3.1.4.17 guanosine 3',5'-cyclic phosphate + H2O - Rattus norvegicus Sprague-Dawley guanosine 5'-phosphate - ? 364651 3.1.4.17 nucleoside 3',5'-cyclic phosphate + H2O - Cavia porcellus nucleoside 5'-phosphate - ? 93632 3.1.4.17 nucleoside 3',5'-cyclic phosphate + H2O - Mammalia nucleoside 5'-phosphate - ? 93632 3.1.4.17 nucleoside 3',5'-cyclic phosphate + H2O - Mus musculus nucleoside 5'-phosphate - ? 93632 3.1.4.17 nucleoside 3',5'-cyclic phosphate + H2O - Homo sapiens nucleoside 5'-phosphate - ? 93632 3.1.4.17 nucleoside 3',5'-cyclic phosphate + H2O - Rattus norvegicus nucleoside 5'-phosphate - ? 93632 3.1.4.17 nucleoside 3',5'-cyclic phosphate + H2O - Bos taurus nucleoside 5'-phosphate - ? 93632 3.1.4.17 nucleoside 3',5'-cyclic phosphate + H2O - Neurospora crassa nucleoside 5'-phosphate - ? 93632 3.1.4.17 nucleoside 3',5'-cyclic phosphate + H2O - Canis lupus familiaris nucleoside 5'-phosphate - ? 93632 3.1.4.17 nucleoside 3',5'-cyclic phosphate + H2O - Xenopus laevis nucleoside 5'-phosphate - ? 93632 3.1.4.17 nucleoside 3',5'-cyclic phosphate + H2O - Sinorhizobium fredii nucleoside 5'-phosphate - ? 93632 3.1.4.17 3',5'-cAMP + H2O 3',5'-cAMP is completely hydrolyzed to adenosine when incubated with PdeA for a prolonged time Myxococcus xanthus 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O 3',5'-cAMP is predominantly hydrolyzed by PDE3A Homo sapiens 5'-AMP - ? 93634 3.1.4.17 additional information 3',5'-cGMP is either not hydrolysed or is cleaved very slowly by PdeA and PdeB, PdeA and PdeB show no activities toward 2'-AMP and 2'-GMP Myxococcus xanthus ? - ? 89 3.1.4.17 3',5'-cAMP + H2O activity with 3',5'-cGMP and 3',5'-cAMP is nearly identical, PDE11 Homo sapiens 5'-AMP - ? 93634 3.1.4.17 3',5'-cGMP + H2O activity with 3',5'-cGMP and 3',5'-cAMP is nearly identical, PDE11 Homo sapiens 5'-GMP - ? 93636 3.1.4.17 3',5'-cAMP + H2O activity with 3',5'-cGMP and 3',5'-cAMP is nearly identical, PDE2 Homo sapiens 5'-AMP - ? 93634 3.1.4.17 3',5'-cGMP + H2O activity with 3',5'-cGMP and 3',5'-cAMP is nearly identical, PDE2 Homo sapiens 5'-GMP - ? 93636 3.1.4.17 3',5'-cAMP + H2O activity with 3',5'-cGMP is higher than with 3',5'-cAMP Homo sapiens 5'-AMP - ? 93634 3.1.4.17 3',5'-cGMP + H2O activity with 3',5'-cGMP is higher than with 3',5'-cAMP Homo sapiens 5'-GMP - ? 93636 3.1.4.17 additional information cGMP levels in the spinal cord are controlled by a number of enzyme isoforms, that can be present in the same cell. Role of NO-cGMP signaling in nociceptive processing Rattus norvegicus ? - ? 89 3.1.4.17 additional information cytoplasmic isoform PDE1A regulates myosin light chain phosphorylation with little effect on apoptosis, whereas nuclear enzyme isoform PDE1A is important in vascular smoth muscle cell growth and survival Rattus norvegicus ? - ? 89 3.1.4.17 cAMP + H2O degradation of extracellular cAMP by isoforms PDE1, PDE7. Degradation of intracellular cAMP by isoform PDE2 Dictyostelium discoideum 5'-AMP - ? 391562 3.1.4.17 cGMP + H2O degradation of intracellular cGMP by isoform PDE3 Dictyostelium discoideum 5'-GMP - ? 391615 3.1.4.17 additional information enzyxme displays dual specificity for hydrolysis of both cAMP and cGMP Saccharomyces cerevisiae ? - ? 89 3.1.4.17 additional information h-prune activity is suppressed by dipyridamole and enhanced by the interaction with nm23-H1. Activity is involved in promoting cancer metastasis Homo sapiens ? - ? 89 3.1.4.17 3'-AMP + H2O high activity Myxococcus xanthus ? - ? 93697 3.1.4.17 3'-GMP + H2O high activity Myxococcus xanthus ? - ? 399563 3.1.4.17 5'-UMP + H2O high activity Myxococcus xanthus ? - ? 399739 3.1.4.17 3',5'-cGMP + H2O highest affinity for 3',5'-cGMP Mus musculus 5'-GMP - ? 93636 3.1.4.17 3',5'-cGMP + H2O highest affinity for 3',5'-cGMP Homo sapiens 5'-GMP - ? 93636 3.1.4.17 2',3'-cAMP + H2O in PdeB the phosphodiesterase activity for 2',3'-cAMP is about 1.7fold higher than that for 3',5'-cAMP Myxococcus xanthus 3'-AMP - ? 399514 3.1.4.17 3',5'-cAMP + H2O inhibition of cAMP hydrolysis by cGMP is likely to contribute to cGMP-mediated signaling in myocardium Homo sapiens 5'-AMP - ? 93634 3.1.4.17 additional information insulin-induced activation of the membrane bound PDE3B is a key step in insulin-mediated inhibition of lipolysis and is also involved in the regulation of insulin-mediated glucose uptake and lipogenesis in adipocytes Rattus norvegicus ? - ? 89 3.1.4.17 additional information isoform PDE1B2 predominantly regulates cGMP and plays a lesser role in cAMP regulation in response to cyclase agonists Homo sapiens ? - ? 89 3.1.4.17 additional information isoform PDE2 regulates the basal cGMP concentration in thalamic neuron Mus musculus ? - ? 89 3.1.4.17 additional information isoforms PDE1 and PDE7 are dual-specificity enzymes with negative cooperativity and very different kinetics. Both have 2-3fold lower affinity for cGMP than for cAMP Dictyostelium discoideum ? - ? 89 3.1.4.17 5'-dAMP + H2O low activity Myxococcus xanthus ? - ? 73575 3.1.4.17 3',5'-cAMP + H2O maximal velocity for cAMP is 4fold to 10fold higher than for cGMP, PDE11A4 Homo sapiens 5'-AMP - ? 93634 3.1.4.17 3',5'-cGMP + H2O maximal velocity for cAMP is 4fold to 10fold higher than for cGMP, PDE11A4 Homo sapiens 5'-GMP - ? 93636 3.1.4.17 additional information muscarinic antagonists acting possibly as inverse agonists on M2/M3 muscarinic acetylcholine receptors anchored to sarcolemma membranes can initiate a new signal transducing cascade leading to inhibition of PDEI, which produces a simultaneous rise in both cAMP and cGMP intracellular levels in tracheal smooth muscle Bos taurus ? - ? 89 3.1.4.17 additional information NAD+ is not a substrate of PDE3A Homo sapiens ? - ? 89 3.1.4.17 additional information not: 2',3'-cAMP Neurospora crassa ? - ? 89 3.1.4.17 additional information not: 2',3'-cAMP Sinorhizobium fredii ? - ? 89 3.1.4.17 additional information overview on physiological role Mammalia ? - ? 89 3.1.4.17 additional information overview on physiological role Bos taurus ? - ? 89 3.1.4.17 3',5'-cAMP + H2O PDE1 Bos taurus adenosine 5'-phosphate - ? 383796 3.1.4.17 3',5'-cGMP + H2O PDE1 Bos taurus guanosine 5'-phosphate - ? 383021 3.1.4.17 3',5'-cGMP + H2O PDE1 and PDE2 are the main PDEs that act to degrade cGMP in methacholine-stimulated cells Oryctolagus cuniculus 5'-GMP - ? 93636 3.1.4.17 additional information PDE1 in Pukinje neurons may play a role in limiting the cGMP-mediated long-term depression response Mus musculus ? - ? 89 3.1.4.17 additional information PDE10A regulates both cAMP and cGMP signaling cascades to impact early signal processing in the corticostriatothalamic circuit Rattus norvegicus ? - ? 89 3.1.4.17 additional information PDE1A is involved in cGMP breakdown in parotid acinar cells of Oryctolagus cuniculus Oryctolagus cuniculus ? - ? 89 3.1.4.17 additional information PDE1A_v7 is the major form of cyclic nucleotide phosphodiesterase 1A expressed in mature sperm and is therefore likely to play an important role in cyclic nucleotide regulation of mature sperm function Mus musculus ? - ? 89 3.1.4.17 3',5'-cAMP + H2O PDE2 Bos taurus adenosine 5'-phosphate - ? 383796 3.1.4.17 additional information PDE2 can hydrolyze both cAMP and cGMP with similar maximal rates. PDE1A prefers cGMP, PDE1B cAMP, and PDE1C hydrolyzes both substrates equally. The PDE3 family can hydrolyze both cAMP and cGMP Homo sapiens ? - ? 89 3.1.4.17 3',5'-cAMP + H2O PDE3A Bos taurus adenosine 5'-phosphate - ? 383796 3.1.4.17 3',5'-cGMP + H2O PDE3A Bos taurus guanosine 5'-phosphate - ? 383021 3.1.4.17 additional information PDE3A physically and functionally interacts with cystic fibrosis transmembrane conductance regulator, CFTR, channel Homo sapiens ? - ? 89 3.1.4.17 additional information PDE3A physically and functionally interacts with cystic fibrosis transmembrane conductance regulator, CFTR, channel Sus scrofa ? - ? 89 3.1.4.17 3',5'-cAMP + H2O PDE3B Bos taurus adenosine 5'-phosphate - ? 383796 3.1.4.17 3',5'-cGMP + H2O PDE3B Bos taurus guanosine 5'-phosphate - ? 383021 3.1.4.17 additional information PdeA and PdeB hydrolyze 3',5'- and 2',3'-cyclic AMP to adenosine, and also demonstrate phosphatase activity toward nucleoside 5'-tri-, 5'-di-, 5'- and 3'-monophosphates with highest activities for nucleoside 5'-monophosphates Myxococcus xanthus ? - ? 89 3.1.4.17 additional information real-time monitoring of PDE2 activity by a fluorescent cAMP sensor shows that activation of PDE2 results in a rapid decrease of intracellular cAMP from high micromolar to sub-micromolar range within a few seconds. High catalytic activity and fast action in regulating cAMP signaling in a physiological system Bos taurus ? - ? 89 3.1.4.17 additional information Rv0805 protein could have an important role to play in regulating cAMP levels in Mycobacterium tuberculosis Mycobacterium tuberculosis ? - ? 89 3.1.4.17 3',5'-cAMP + H2O specific for 3',5'-cGMP and 3',5'-cAMP, PDE1 Drosophila melanogaster 5'-AMP - ? 93634 3.1.4.17 3',5'-cGMP + H2O specific for 3',5'-cGMP and 3',5'-cAMP, PDE1 Drosophila melanogaster 5'-GMP - ? 93636 3.1.4.17 3',5'-cAMP + H2O specific for 3',5'-cGMP and 3',5'-cAMP, PDE11 Drosophila melanogaster 5'-AMP - ? 93634 3.1.4.17 3',5'-cGMP + H2O specific for 3',5'-cGMP and 3',5'-cAMP, PDE11 Drosophila melanogaster 5'-GMP - ? 93636 3.1.4.17 additional information splice variants PDE11A1 and PDE11A2 show higher affinity for cAMP and cGMP than PDE11A4 Homo sapiens ? - ? 89 3.1.4.17 additional information structure analysis of phosphodiesterase 10 binding with cAMP and cGMP by hybrid quantum mechanical/molecular mechanical calculations using cyrstal structures of the substrate-bound enzyme, detailed overview Homo sapiens ? - ? 89 3.1.4.17 additional information substrate specificity of PdeE, overview Myxococcus xanthus ? - ? 89 3.1.4.17 additional information the effect of increasing concentrations of cGMP on endothelial permeability is biphasic, attributable to the relative amounts of enzyme isoforms PDE2A and PDE3A in endothelial cells Homo sapiens ? - ? 89 3.1.4.17 3',5'-cAMP + H2O the enzyme is preferentially degrading linear dinucleotides, such as 5'-pApA, 5'-pGpG, and 5'-pApG, compared with cyclic dinucleotide substrates Thermotoga maritima 5'-AMP - ? 93634 3.1.4.17 3',5'-cAMP + H2O the enzyme is preferentially degrading linear dinucleotides, such as 5'-pApA, 5'-pGpG, and 5'-pApG, compared with cyclic dinucleotide substrates Thermotoga maritima DSM 3109 5'-AMP - ? 93634 3.1.4.17 additional information the enzyme is preferentially degrading linear dinucleotides, such as 5'-pApA, 5'-pGpG, and 5'-pApG, compared with cyclic dinucleotide substrates Thermotoga maritima ? - ? 89 3.1.4.17 additional information the enzyme is preferentially degrading linear dinucleotides, such as 5'-pApA, 5'-pGpG, and 5'-pApG, compared with cyclic dinucleotide substrates Thermotoga maritima DSM 3109 ? - ? 89 3.1.4.17 additional information the enzyme is unable to dephosphorylate 5'-AMP Corynebacterium glutamicum ? - ? 89 3.1.4.17 additional information the enzyme is unable to dephosphorylate 5'-AMP Corynebacterium glutamicum ATCC 13032 ? - ? 89 3.1.4.17 additional information the enzyme might be involved in multiple physiological processes in various organs via its ability to modulate intracellular cAMP and cGMP levels Homo sapiens ? - ? 89 3.1.4.17 3',5'-cGMP + H2O the technique can be used to measure Ca2+/CaM-stimulated PDE activity in cultured cells or tissues Rattus norvegicus 5'-GMP - ? 93636 3.1.4.17 UDP-D-glucose + H2O UDP-D-glucose is hydrolyzed by PdeB, but not by PdeA Myxococcus xanthus ? - ? 402105