3.1.26.8	5S precursor rRNA + H2O	-	Bacillus subtilis	mature 5S rRNA	-	?	370828	
3.1.26.8	5S precursor rRNA + H2O	-	Geobacillus stearothermophilus	mature 5S rRNA	-	?	370828	
3.1.26.8	5S precursor rRNA + H2O	enzyme is responsible for maturation of 5S rRNA	Bacillus subtilis	mature 5S rRNA	-	?	370828	
3.1.26.8	5S precursor rRNA + H2O	in contrast to the Escherichia coli enzyme, the Bacillus subtilis enzyme cleaves the 5S rRNA in a double-stranded region that extends from the L5 arm in one step	Bacillus subtilis	mature 5S rRNA	-	?	370828	
3.1.26.8	5S precursor rRNA + H2O	5S rRNA maturation	Geobacillus stearothermophilus	mature 5S rRNA	-	?	370828	
3.1.26.8	5S rRNA precursor + H2O	-	Bacillus sp. (in: Bacteria)	mature 5S rRNA + oligonucleotides	-	?	93123	
3.1.26.8	5S rRNA precursor + H2O	the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA	Bacillus subtilis	mature 5S rRNA + oligonucleotides	the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA	?	93123	
3.1.26.8	5S rRNA precursor + H2O	symmetrically arranged sequences are not used in the RNase M5 interaction	Bacillus subtilis	mature 5S rRNA + oligonucleotides	-	?	93123	
3.1.26.8	5S rRNA precursor + H2O	electrostatic interactions contribute about 65% to the overall binding energy	Bacillus subtilis	mature 5S rRNA + oligonucleotides	-	?	93123	
3.1.26.8	5S rRNA precursor + H2O	recognition elements in potential RNase M5 substrates mainly reside in the mature moiety of the precursor. The enzyme recognizes the overall conformation of potential substrates	Bacillus subtilis	mature 5S rRNA + oligonucleotides	-	?	93123	
3.1.26.8	5S rRNA precursor + H2O	the enzyme attacks a ribonucleoprotein substrate consisting of protein BL16 in complex with the 5S rRNA precursor	Bacillus subtilis	mature 5S rRNA + oligonucleotides	-	?	93123	
3.1.26.8	5S rRNA precursor + H2O	the enzyme cleaves twice in double-helical region of a 179-nucleotide precursor of 5S rRNA to yield mature 5S rRNA of 116 nucleotides plus fragments of 21 and 42 nucleotides. The conformation, not sequence, is the important feature in the locale of the cleaved bonds	Bacillus subtilis	mature 5S rRNA + oligonucleotides	-	?	93123	
3.1.26.8	5S rRNA precursor + H2O	the 5'-precursor-specific segment contains one or more components recognized by RNase M5 during its interaction with the p5A substrate	Bacillus subtilis	mature 5S rRNA + oligonucleotides	-	?	93123	
3.1.26.8	5S rRNA precursor + H2O	the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA	Bacillus subtilis 168	mature 5S rRNA + oligonucleotides	the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA	?	93123	
3.1.26.8	5S rRNA precursor + H2O	-	Bacillus sp. (in: Bacteria) Q	mature 5S rRNA + oligonucleotides	-	?	93123	
3.1.26.8	5S rRNA precursor + H2O	responsible for terminal maturation of 5S rRNA	Bacillus subtilis	?	-	?	369732	
3.1.26.8	5S rRNA precursor + H2O	responsible for terminal maturation of 5S rRNA	Bacillus subtilis 168	?	-	?	369732	
3.1.26.8	artificial 5S rRNA precursor + H2O	-	Bacillus subtilis	?	-	?	453976	
3.1.26.8	artificial 5S rRNA precursor substrate in complex with BL16 subunit + H2O	-	Bacillus subtilis	?	-	?	453975	
3.1.26.8	additional information	the artificial substrate lacking the 5'-terminal precursor segment is cleaved very much more slowly than that lacking 3' segment	Bacillus subtilis	?	-	?	89	
3.1.26.8	additional information	the enzyme is highly conserved among G + C Gram-positive bacteria, phylogenetic distribution	Bacillus subtilis	?	-	?	89	
3.1.26.8	additional information	mRNAs are poor or no substrates	Bacillus subtilis	?	-	?	89	
3.1.26.8	RNA + H2O	-	Bacillus subtilis	?	-	?	141227	
3.1.26.8	RNA in complex with BL16 subunit + H2O	-	Bacillus subtilis	?	-	?	452632