3.1.2.12 2-naphthyl acetate + H2O artificial substrate of YeiG Escherichia coli 2-naphthol + acetate - ? 36028 3.1.2.12 2-naphthyl acetate + H2O artificial substrate of YeiG Escherichia coli BW25113 2-naphthol + acetate - ? 36028 3.1.2.12 4-methylumbelliferyl acetate + H2O - Arabidopsis thaliana 4-methylumbelliferol + acetate - ? 363951 3.1.2.12 4-methylumbelliferyl acetate + H2O - Homo sapiens 4-methylumbelliferone + acetate - ? 64574 3.1.2.12 4-methylumbelliferyl acetate + H2O - Arabidopsis thaliana 4-methylumbelliferone + acetate - ? 64574 3.1.2.12 4-methylumbelliferyl acetate + H2O very low activity Rattus norvegicus 4-methylumbelliferone + acetate - ? 64574 3.1.2.12 4-methylumbelliferyl acetate + H2O very low activity Saccharomyces cerevisiae 4-methylumbelliferone + acetate - ? 64574 3.1.2.12 4-methylumbelliferyl acetate + H2O very low activity Lota maculosa 4-methylumbelliferone + acetate - ? 64574 3.1.2.12 4-nitrophenyl acetate + H2O - Homo sapiens 4-nitrophenol + acetate - ? 12514 3.1.2.12 4-nitrophenyl acetate + H2O - Saccharomyces cerevisiae 4-nitrophenol + acetate - ? 12514 3.1.2.12 4-nitrophenyl acetate + H2O - Arabidopsis thaliana 4-nitrophenol + acetate - ? 12514 3.1.2.12 4-nitrophenyl acetate + H2O - Agrobacterium tumefaciens 4-nitrophenol + acetate - ? 12514 3.1.2.12 4-nitrophenyl acetate + H2O - Pseudoalteromonas haloplanktis 4-nitrophenol + acetate - ? 12514 3.1.2.12 4-nitrophenyl acetate + H2O artificial substrate of FrmB and YeiG Escherichia coli 4-nitrophenol + acetate - ? 12514 3.1.2.12 4-nitrophenyl acetate + H2O - Pseudoalteromonas haloplanktis TAC 125 4-nitrophenol + acetate - ? 12514 3.1.2.12 4-nitrophenyl acetate + H2O artificial substrate of FrmB and YeiG Escherichia coli BW25113 4-nitrophenol + acetate - ? 12514 3.1.2.12 4-nitrophenyl butyrate + H2O - Saccharomyces cerevisiae 4-nitrophenol + butyrate - ? 115376 3.1.2.12 4-nitrophenyl butyrate + H2O highest activity Agrobacterium tumefaciens 4-nitrophenol + butyrate - ? 115376 3.1.2.12 4-nitrophenyl butyrate + H2O artificial substrate of YeiG Escherichia coli 4-nitrophenol + butyrate - ? 115376 3.1.2.12 4-nitrophenyl butyrate + H2O hydrolysis of chiral substrates (4-chloro-3-hydroxybutyl acetate, 1-phenylethyl acetate, methyl mandelate) observed, esterification with n-propanol of chiral substrates (ketoprofen, ibuprofen) observed, esterification with butyl acid of chiral substrate (2-octanol) observed Pseudomonas putida 4-nitrophenol + butyrate - ? 115376 3.1.2.12 4-nitrophenyl caproate + H2O - Agrobacterium tumefaciens 4-nitrophenol + caproate - ? 363887 3.1.2.12 4-nitrophenyl caproate + H2O artificial substrate of YeiG Escherichia coli 4-nitrophenol + caproate - ? 363887 3.1.2.12 4-nitrophenyl propionate + H2O - Agrobacterium tumefaciens 4-nitrophenol + propionate - ? 115374 3.1.2.12 4-nitrophenyl propionate + H2O artificial substrate of FrmB and YeiG Escherichia coli 4-nitrophenol + propionate - ? 115374 3.1.2.12 4-nitrophenyl thioacetate + H2O substrate with small acyl moiety Pseudoalteromonas haloplanktis 4-nitrophenol + thioacetate - ? 411380 3.1.2.12 4-nitrophenyl thioacetate + H2O substrate with small acyl moiety Pseudoalteromonas haloplanktis TAC 125 4-nitrophenol + thioacetate - ? 411380 3.1.2.12 4-methyl-2-oxo-2H-chromen-7-yl (2E)-but-2-enoate + H2O - Arabidopsis thaliana ? - ? 390809 3.1.2.12 4-methyl-2-oxo-2H-chromen-7-yl 3,3-dimethylbutanoate + H2O - Arabidopsis thaliana ? - ? 390810 3.1.2.12 4-methyl-2-oxo-2H-chromen-7-yl 3-cyclopentylpropanoate + H2O - Arabidopsis thaliana ? - ? 390811 3.1.2.12 4-methyl-2-oxo-2H-chromen-7-yl 3-methylbut-2-enoate + H2O - Arabidopsis thaliana ? - ? 390812 3.1.2.12 4-methyl-2-oxo-2H-chromen-7-yl 3-methylbutanoate + H2O - Arabidopsis thaliana ? - ? 390813 3.1.2.12 4-methyl-2-oxo-2H-chromen-7-yl 3-phenoxybutanoate + H2O - Arabidopsis thaliana ? - ? 390814 3.1.2.12 4-methyl-2-oxo-2H-chromen-7-yl acetate + H2O - Arabidopsis thaliana ? - ? 390815 3.1.2.12 4-methyl-2-oxo-2H-chromen-7-yl butyrate + H2O - Arabidopsis thaliana ? - ? 390816 3.1.2.12 4-methyl-2-oxo-2H-chromen-7-yl cyclopentanecarboxylate + H2O - Arabidopsis thaliana ? - ? 390817 3.1.2.12 4-methyl-2-oxo-2H-chromen-7-yl heptanoate + H2O - Arabidopsis thaliana ? - ? 390818 3.1.2.12 4-methyl-2-oxo-2H-chromen-7-yl hexanoate + H2O - Arabidopsis thaliana ? - ? 390819 3.1.2.12 4-methyl-2-oxo-2H-chromen-7-yl pentanoate + H2O - Arabidopsis thaliana ? - ? 390820 3.1.2.12 4-methyl-2-oxo-2H-chromen-7-yl pivalate + H2O - Arabidopsis thaliana ? - ? 390821 3.1.2.12 4-methyl-2-oxo-2H-chromen-7-yl propionate + H2O - Arabidopsis thaliana ? - ? 390822 3.1.2.12 fluorescein diacetate + H2O - Arabidopsis thaliana ? - ? 364426 3.1.2.12 additional information 2-nitrophenyl acetate is a poor substrate Arabidopsis thaliana ? - ? 89 3.1.2.12 additional information no activity by FrmB and YeiG with S-(1,2-dicarboxyethyl)glutathione, S-hexylglutathione, and S-(4-nitrobenzyl)glutathione Escherichia coli ? - ? 89 3.1.2.12 additional information the enzyme is a serine hydrolase rather than a cysteine hydrolase Arabidopsis thaliana ? - ? 89 3.1.2.12 additional information esterase D/S-formylglutathione hydrolase plays a key role in glutathione-dependent detoxification of endogenously formed formaldehyde as well as in hydrolysis of various xenobiotics Homo sapiens ? - ? 89 3.1.2.12 additional information is unable to hydrolyse alpha-naphthyl dodecanoate, 4-nitrophenyl caprate, 4-nitrophenyl laurate, and 4-nitrophenyl palmitate. Potential role for Cys54 in regulation of enzyme activity through S-glutathionylation. Hydrolyzes C-O bonds with high affinity toward short to medium chain esters (C3-C4). Has more affinity toward C3 and C4 4-nitrophenyl esters than toward C3 and C4 alpha-naphthyl esters Agrobacterium tumefaciens ? - ? 89 3.1.2.12 additional information PhEst contains a catalytic triad formed by residues Ser147, Asp225 and His258. It shows no activity toward substrates with bulky acyl groups such as S-lactoylglutathione. Has a very narrow acyl-binding pocket in a typical alpha/beta-hydrolase fold Pseudoalteromonas haloplanktis ? - ? 89 3.1.2.12 additional information enzyme shows modest activity with substrates 4-nitrophenyl acetate, 4-nitrophenyl butanoate, but not with 4-nitrophenyl trimethylacetate Staphylococcus aureus ? - - 89 3.1.2.12 additional information PhEst contains a catalytic triad formed by residues Ser147, Asp225 and His258. It shows no activity toward substrates with bulky acyl groups such as S-lactoylglutathione. Has a very narrow acyl-binding pocket in a typical alpha/beta-hydrolase fold Pseudoalteromonas haloplanktis TAC 125 ? - ? 89 3.1.2.12 additional information no activity by FrmB and YeiG with S-(1,2-dicarboxyethyl)glutathione, S-hexylglutathione, and S-(4-nitrobenzyl)glutathione Escherichia coli BW25113 ? - ? 89 3.1.2.12 additional information enzyme shows modest activity with substrates 4-nitrophenyl acetate, 4-nitrophenyl butanoate, but not with 4-nitrophenyl trimethylacetate Staphylococcus aureus PS 47 ? - - 89 3.1.2.12 S-formylglutathione + H2O - [Candida] boidinii ? - ? 369649 3.1.2.12 S-formylglutathione + H2O dissimilatory pathway during growth on methanol [Candida] boidinii ? - ? 369649 3.1.2.12 S-formylglutathione + H2O part of the glutathione-dependent formaldehyde oxidation pathway Paracoccus denitrificans ? - ? 369649 3.1.2.12 S-formylglutathione + H2O involved in the oxidation of methanol by methylotrophic yeasts Kloeckera sp. ? - ? 369649 3.1.2.12 S-formylglutathione + H2O detoxification of formaldehyde Saccharomyces cerevisiae ? - ? 369649 3.1.2.12 alpha-naphthyl acetate + H2O - Escherichia coli alpha-naphthol + acetate - ? 36052 3.1.2.12 alpha-naphthyl acetate + H2O - Saccharomyces cerevisiae alpha-naphthol + acetate - ? 36052 3.1.2.12 alpha-naphthyl acetate + H2O - Arabidopsis thaliana alpha-naphthol + acetate - ? 36052 3.1.2.12 alpha-naphthyl acetate + H2O - Escherichia coli BW25113 alpha-naphthol + acetate - ? 36052 3.1.2.12 alpha-naphthyl butyrate + H2O - Agrobacterium tumefaciens alpha-naphthol + butyrate - ? 363970 3.1.2.12 alpha-naphthyl propionate + H2O - Agrobacterium tumefaciens alpha-naphthol + propionate - ? 364342 3.1.2.12 beta-naphthyl acetate + H2O - Arabidopsis thaliana beta-naphthol + acetate - ? 364353 3.1.2.12 carboxyfluorescein diacetate + H2O - Saccharomyces cerevisiae carboxyfluorescein + acetate - ? 64577 3.1.2.12 (S)-D-lactoylglutathione + H2O - Staphylococcus aureus D-lactate + glutathione - ? 452605 3.1.2.12 (S)-D-lactoylglutathione + H2O - Staphylococcus aureus PS 47 D-lactate + glutathione - ? 452605 3.1.2.12 paraoxon + H2O - Saccharomyces cerevisiae diethylphosphate + 4-nitrophenol - ? 36030 3.1.2.12 fluorescein-3',6'-bis(oxy)methylene bis[(methylsulfanyl)acetate] + H2O - Staphylococcus aureus fluorescein-3'-oxymethyl (methylsulfanyl)acetate + (methylsulfanyl)acetate - ? 459678 3.1.2.12 fluorescein-3',6'-bis(oxy)methylene bis(2-methoxypropanoate) + H2O - Staphylococcus aureus fluorescein-3'-oxymethyl 2-methoxypropanoate + 2-methoxypropanoate - ? 459673 3.1.2.12 fluorescein-3',6'-bis(oxy)methylene bis(2-methoxypropanoate) + H2O - Staphylococcus aureus PS 47 fluorescein-3'-oxymethyl 2-methoxypropanoate + 2-methoxypropanoate - ? 459673 3.1.2.12 fluorescein-3',6'-bis(oxy)methylene dibutanoate + H2O - Staphylococcus aureus fluorescein-3'-oxymethyl butanoate + butanoate - ? 459680 3.1.2.12 fluorescein-3',6'-bis(oxy)methylene dihexanoate + H2O - Staphylococcus aureus fluorescein-3'-oxymethyl hexanoate + hexanoate - ? 459681 3.1.2.12 fluorescein-3',6'-bis(oxy)methylene bis(methoxyacetate) + H2O - Staphylococcus aureus fluorescein-3'-oxymethyl methoxyacetate + methoxyacetate - ? 459675 3.1.2.12 fluorescein-3',6'-bis(oxy)methylene dipentanoate + H2O - Staphylococcus aureus fluorescein-3'-oxymethyl pentanoate + pentanoate - ? 459682 3.1.2.12 fluorescein-3',6'-bis(oxy)methylene bis(propoxyacetate) + H2O - Staphylococcus aureus fluorescein-3'-oxymethyl propoxyacetate + propoxyacetate - ? 459676 3.1.2.12 fluorescein-3',6'-bis(oxy)methylene bis[[(propan-2-yl)sulfanyl]acetate] + H2O - Staphylococcus aureus fluorescein-3'-oxymethyl [(propan-2-yl)sulfanyl]acetate + [(propan-2-yl)sulfanyl]acetate - ? 459679 3.1.2.12 fluorescein-3',6'-bis(oxy)methylene bis(ethoxyacetate) + H2O - Staphylococcus aureus fluorescein-3'-yoxymethyl ethoxyacetate + ethoxyacetate - ? 459674 3.1.2.12 fluorescein-3',6'-bis(oxy)methylene bis(ethoxyacetate) + H2O - Staphylococcus aureus PS 47 fluorescein-3'-yoxymethyl ethoxyacetate + ethoxyacetate - ? 459674 3.1.2.12 S-acetylglutathione + H2O - Homo sapiens glutathione + acetate - ? 64573 3.1.2.12 S-acetylglutathione + H2O - Rattus norvegicus glutathione + acetate - ? 64573 3.1.2.12 S-acetylglutathione + H2O - Arabidopsis thaliana glutathione + acetate - ? 64573 3.1.2.12 S-acetylglutathione + H2O - Lota maculosa glutathione + acetate - ? 64573 3.1.2.12 S-acetylglutathione + H2O slight activity Homo sapiens glutathione + acetate - ir 64573 3.1.2.12 S-acetylglutathione + H2O 1.3% of reaction rate with S-formylglutathione [Candida] boidinii glutathione + acetate - ? 64573 3.1.2.12 S-acetylglutathione + H2O substrate with small acyl moiety, high activity Pseudoalteromonas haloplanktis glutathione + acetate - ? 64573 3.1.2.12 S-acetylglutathione + H2O substrate with small acyl moiety, high activity Pseudoalteromonas haloplanktis TAC 125 glutathione + acetate - ? 64573 3.1.2.12 S-formylglutathione + H2O - Paracoccus denitrificans glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Mus musculus glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Escherichia coli glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Homo sapiens glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Homo sapiens glutathione + formate - ir 64570 3.1.2.12 S-formylglutathione + H2O - Rattus norvegicus glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Saccharomyces cerevisiae glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Pisum sativum glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Arabidopsis thaliana glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Komagataella pastoris glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - [Candida] boidinii glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Ogataea angusta glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Kloeckera sp. glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Schwanniomyces vanrijiae glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Dipodascus klebahnii glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Lota maculosa glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Ogataea trehalophila glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Torulopsis pinus glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O enzyme is involved in formaldehyde detoxification Arabidopsis thaliana glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O enzyme is involved in the glutathione-dependent formaldehyde oxidation pathway during growth on methanol as the sole carbon source, regulation of gene expression [Candida] boidinii glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O key enzyme catalyzes the final step of the formaldehyde detoxification, glutathione recycling Arabidopsis thaliana glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O key enzyme of the formaldehyde detoxification, glutathione recycling Arabidopsis thaliana glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O the substrate is an intermediate in the glutathione-dependent formaldehyde detoxification pathway Escherichia coli glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O best substrate of FrmB and YeiG Escherichia coli glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O substrate with small acyl moiety, lower activity than compared to S-acetylglutathione Pseudoalteromonas haloplanktis glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O substrate with small acyl moiety, lower activity than compared to S-acetylglutathione Pseudoalteromonas haloplanktis TAC 125 glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O - Komagataella pastoris IFP 206 glutathione + formate - ? 64570 3.1.2.12 S-formylglutathione + H2O the substrate is an intermediate in the glutathione-dependent formaldehyde detoxification pathway Escherichia coli BW25113 glutathione + formate - ? 64570 3.1.2.12 S-lactoylglutathione + H2O - Saccharomyces cerevisiae glutathione + lactate - ? 64591 3.1.2.12 S-lactoylglutathione + H2O substrate of FrmB and YeiG Escherichia coli glutathione + lactate - ? 64591 3.1.2.12 p-nitrophenyl acetate + H2O very low activity Rattus norvegicus p-nitrophenol + acetate - ? 36053 3.1.2.12 p-nitrophenyl acetate + H2O very low activity Saccharomyces cerevisiae p-nitrophenol + acetate - ? 36053 3.1.2.12 p-nitrophenyl acetate + H2O very low activity Lota maculosa p-nitrophenol + acetate - ? 36053 3.1.2.12 bis(pivaloyloxymethyl)(1-hydroxy-2-oxopiperidin-3-yl)phosphonate + H2O i.e. POM-HEX, substrate is a prodrug of the compound HEX,which inhibits the glycolytic enzyme enolase Staphylococcus aureus [[(hydroxymethoxy)(1-hydroxy-2-oxopiperidin-3-yl)phosphoryl]oxy]methyl 2,2-dimethylpropanoate + 2,2-dimethylpropanoic acid - ? 459499 3.1.2.12 bis(pivaloyloxymethyl)(1-hydroxy-2-oxopiperidin-3-yl)phosphonate + H2O i.e. POM-HEX, substrate is a prodrug of the compound HEX,which inhibits the glycolytic enzyme enolase Staphylococcus aureus PS 47 [[(hydroxymethoxy)(1-hydroxy-2-oxopiperidin-3-yl)phosphoryl]oxy]methyl 2,2-dimethylpropanoate + 2,2-dimethylpropanoic acid - ? 459499