3.1.1.96	D-aminoacyl-tRNA + H2O	-	Escherichia coli	D-amino acid + tRNA	-	?	429228	
3.1.1.96	D-aminoacyl-tRNA + H2O	-	Azotobacter vinelandii	D-amino acid + tRNA	-	?	429228	
3.1.1.96	D-aminoacyl-tRNA + H2O	-	Haemophilus influenzae	D-amino acid + tRNA	-	?	429228	
3.1.1.96	D-aminoacyl-tRNA + H2O	the enzyme recognize almost all D-aminoacyl-tRNAs instead of only D-Tyr-tRNATyr	Escherichia coli	D-amino acid + tRNA	-	?	429228	
3.1.1.96	D-aspartyl-tRNAAsp + H2O	initial rate of hydrolysis is very close to that obtained with D-tyrosyl-tRNATyr	Arabidopsis thaliana	D-aspartate + tRNAAsp	-	?	426182	
3.1.1.96	D-aspartyl-tRNAAsp + H2O	no activity with L-aspartyl-tRNAAsp	Escherichia coli	D-aspartate + tRNAAsp	-	?	426182	
3.1.1.96	D-tryptophanyl-tRNATrp + H2O	-	Escherichia coli	D-tryptophan + tRNATrp	-	?	426256	
3.1.1.96	D-Tyr-tRNA + H2O	-	Escherichia coli	D-Tyr + tRNA	-	?	429234	
3.1.1.96	D-Tyr-tRNA + H2O	-	Escherichia coli K-37	D-Tyr + tRNA	-	?	429234	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	-	Escherichia coli	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	-	Saccharolobus solfataricus	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	-	Archaeoglobus fulgidus	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	-	Synechocystis sp.	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	-	Thermus thermophilus	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	-	Bacillus amyloliquefaciens	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	the enzyme can recycle misaminoacylated D-Tyr-tRNATyr	Saccharolobus solfataricus	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	the enzyme can recycle misaminoacylated D-Tyr-tRNATyr	Pyrococcus abyssi	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	the enzyme can recycle misaminoacylated D-Tyr-tRNATyr	Archaeoglobus fulgidus	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	no activity with L-tyrosyl-tRNATyr and diacetyl-L-Lys-tRNALys	Arabidopsis thaliana	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	no activity with L-tyrosyl-tRNATyr, N-acetyl-L-tyrosyl-tRNATyr and N-acetyl-D-tyrosyl-tRNATyr	Escherichia coli	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	the deacylase does not hydrolyze L-Trp-tRNATrp at a significant rate	Escherichia coli	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	the initial rate of D-Tyr-tRNATyr deacylation is at least 200fold higher than that of L-Tyr-tRNATyr	Pyrococcus abyssi	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	the enzyme is responsible for resistance to the non-canonical amino acid D-tyrosine	Bacillus amyloliquefaciens	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	-	Saccharolobus solfataricus P2	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	the enzyme can recycle misaminoacylated D-Tyr-tRNATyr	Saccharolobus solfataricus P2	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	-	Bacillus amyloliquefaciens A50	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	the enzyme is responsible for resistance to the non-canonical amino acid D-tyrosine	Bacillus amyloliquefaciens A50	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	the enzyme can recycle misaminoacylated D-Tyr-tRNATyr	Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	D-tyrosyl-tRNATyr + H2O	the initial rate of D-Tyr-tRNATyr deacylation is at least 200fold higher than that of L-Tyr-tRNATyr	Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543	D-tyrosine + tRNATyr	-	?	424765	
3.1.1.96	Gly-tRNAAla + H2O	-	Escherichia coli	Gly + tRNAAla	-	?	423630	
3.1.1.96	Gly-tRNAAla + H2O	the enzyme has a crucial role in preventing the accumulation of mischarged Gly-tRNAAla species. The enzyme effectively decouples glycine mischarged on tRNAAla, key cellular role as a glycine deacylator	Escherichia coli	Gly + tRNAAla	-	?	423630	
3.1.1.96	Gly-tRNAAla + H2O	the enzyme hydrolyzes D-amino acids mistakenly attached to tRNAs and eliminates glycine that has been erroneously coupled to tRNAAla	Escherichia coli	Gly + tRNAAla	-	?	423630	
3.1.1.96	additional information	comparison of the growth of wild-type and deletion mutant strains indicates protection against the toxicity of D-Trp, D-Asp, D-Ser, and D-Gln afforded by the D-Tyr-tRNATyr deacylase gene. The effect of the other D-amino acids on growth is similar for the wild-type and deletion mutant strains	Escherichia coli	?	-	?	89	
3.1.1.96	additional information	comparison of the growth of wild-type and deletion mutant strains indicates protection against toxicity by D-leucine afforded by the D-Tyr-tRNATyr deacylase gene. Full inhibition of the growth of the Ddtd1 strain is reached at 0.1 mM D-tyrosine. With the DTD1 strain, identical growth inhibition required 0.3 mM D-amino acid. The effect of the other D-amino acids on growth is similar for the wild-type and deletion mutant strains	Saccharomyces cerevisiae	?	-	?	89