3.1.1.41 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(1-methylcyclopropane-1-carboxylate) + H2O - Thermotoga maritima ? - ? 444436 3.1.1.41 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(1-methylcyclopropane-1-carboxylate) + H2O - Thermotoga maritima DSM 3109 ? - ? 444436 3.1.1.41 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(2-methylacrylate) + H2O - Thermotoga maritima ? - ? 444437 3.1.1.41 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(2-methylacrylate) + H2O - Thermotoga maritima DSM 3109 ? - ? 444437 3.1.1.41 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) dicyclobutanecarboxylate + H2O - Thermotoga maritima ? - ? 444438 3.1.1.41 ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) dicyclobutanecarboxylate + H2O - Thermotoga maritima DSM 3109 ? - ? 444438 3.1.1.41 1,2,3,4-tetra-O--acetyl-xylopyranose + H2O - Bacillus subtilis ? - ? 364219 3.1.1.41 1-naphthyl acetate + H2O - Thermotoga maritima 1-naphthol + acetate - ? 12515 3.1.1.41 1-naphthyl acetate + H2O - Thermotoga maritima DSM 3109 1-naphthol + acetate - ? 12515 3.1.1.41 1-naphthyl acetate + H2O - Thermotoga maritima ATCC 43589 1-naphthol + acetate - ? 12515 3.1.1.41 2,3,2',3',4'-penta-acetyl-xylobiose + H2O - Bacillus subtilis ? - ? 364217 3.1.1.41 2-(2-oxo-1,3-dihydroindol-3-yl) acetic acid + H2O - Thermotoga maritima ? - ? 446625 3.1.1.41 2-(2-oxo-1,3-dihydroindol-3-yl) acetic acid + H2O - Thermotoga maritima DSM 3109 ? - ? 446625 3.1.1.41 2-(2-oxo-1,3-dihydroindol-3-yl) acetic acid + H2O - Thermotoga maritima ATCC 43589 ? - ? 446625 3.1.1.41 2-naphthyl acetate + H2O - Bacillus subtilis 2-naphthol + acetate - ? 36028 3.1.1.41 2-naphthyl acetate + H2O - Bacillus subtilis CICC 20034 2-naphthol + acetate - ? 36028 3.1.1.41 3'-O-acetyl-2'-deoxyadenosine + H2O - Bacillus subtilis ? - ? 364218 3.1.1.41 3-naphthyl acetate + H2O - Bacillus subtilis 3-naphthol + acetate - ? 429724 3.1.1.41 4-methylumbelliferyl acetate + H2O - Thermotoga maritima 4-methylumbelliferol + acetate - ? 363951 3.1.1.41 4-methylumbelliferyl acetate + H2O - Bacillus subtilis 4-methylumbelliferol + acetate - ? 363951 3.1.1.41 4-methylumbelliferyl acetate + H2O - Thermotoga maritima DSM 3109 4-methylumbelliferol + acetate - ? 363951 3.1.1.41 4-methylumbelliferyl acetate + H2O - Thermotoga maritima ATCC 43589 4-methylumbelliferol + acetate - ? 363951 3.1.1.41 4-nitrophenyl acetate + H2O - Amycolatopsis lactamdurans 4-nitrophenol + acetate - ? 12514 3.1.1.41 4-nitrophenyl acetate + H2O - Acremonium chrysogenum 4-nitrophenol + acetate - ? 12514 3.1.1.41 4-nitrophenyl acetate + H2O - Bacillus subtilis 4-nitrophenol + acetate - ? 12514 3.1.1.41 4-nitrophenyl acetate + H2O - Thermotoga maritima 4-nitrophenol + acetate - ? 12514 3.1.1.41 4-nitrophenyl acetate + H2O - Amycolatopsis lactamdurans MA4213 4-nitrophenol + acetate - ? 12514 3.1.1.41 4-nitrophenyl acetate + H2O - Thermotoga maritima DSM 3109 4-nitrophenol + acetate - ? 12514 3.1.1.41 4-nitrophenyl acetate + H2O - Acremonium chrysogenum C10 / ATCC 48272 4-nitrophenol + acetate - ? 12514 3.1.1.41 4-nitrophenyl acetate + H2O - Bacillus subtilis CICC 20034 4-nitrophenol + acetate - ? 12514 3.1.1.41 4-nitrophenyl acetate + H2O - Thermotoga maritima ATCC 43589 4-nitrophenol + acetate - ? 12514 3.1.1.41 4-nitrophenyl beta-D-xylopyranoside + H2O 4-nitrophenyl-beta-D-xylopyranoside monoacetates as substrates in a beta-xylosidase-coupled assay, TM0077 hydrolyzes acetate at positions 2, 3 and 4 with equal efficiencyas substrates in a ?-xylosidase-coupled assay Thermotoga maritima 4-nitrophenol + D-xylopyranose - ? 411374 3.1.1.41 4-nitrophenyl butyrate + H2O - Bacillus subtilis 4-nitrophenol + butyrate - ? 115376 3.1.1.41 4-nitrophenyl butyrate + H2O - Bacillus subtilis CICC 20034 4-nitrophenol + butyrate - ? 115376 3.1.1.41 4-nitrophenyl caprylate + H2O - Staphylococcus sp. 4-nitrophenol + caprylate - ? 363889 3.1.1.41 7-amino-cephalosporanic acid + H2O - Thermotoga maritima 7-amino-3-deacetylcephalosporanic acid + acetate - ? 446894 3.1.1.41 7-aminocephalosporanic acid + H2O - Bacillus subtilis acetate + deacetyl-7-aminocephalosporanic acid - ? 115271 3.1.1.41 7-aminocephalosporanic acid + H2O - Rhodotorula glutinis acetate + deacetyl-7-aminocephalosporanic acid - ? 115271 3.1.1.41 7-aminocephalosporanic acid + H2O - Citrus sp. acetate + deacetyl-7-aminocephalosporanic acid - ? 115271 3.1.1.41 7-aminocephalosporanic acid + H2O - Amycolatopsis lactamdurans acetate + deacetyl-7-aminocephalosporanic acid - ? 115271 3.1.1.41 7-aminocephalosporanic acid + H2O - Thermotoga maritima acetate + deacetyl-7-aminocephalosporanic acid - ? 115271 3.1.1.41 7-aminocephalosporanic acid + H2O - Amycolatopsis lactamdurans MA4213 acetate + deacetyl-7-aminocephalosporanic acid - ? 115271 3.1.1.41 7-aminocephalosporanic acid + H2O - Thermotoga maritima DSM 3109 acetate + deacetyl-7-aminocephalosporanic acid - ? 115271 3.1.1.41 7-aminocephalosporanic acid + H2O - Rhodotorula glutinis 38B1 acetate + deacetyl-7-aminocephalosporanic acid - ? 115271 3.1.1.41 7-aminocephalosporanic acid + H2O - Bacillus subtilis WRRL-B-558 acetate + deacetyl-7-aminocephalosporanic acid - ? 115271 3.1.1.41 7-aminocephalosporanic acid + H2O - Bacillus subtilis ATCC 6633 acetate + deacetyl-7-aminocephalosporanic acid - ? 115271 3.1.1.41 7-aminocephalosporanic acid + H2O - Thermotoga maritima ATCC 43589 acetate + deacetyl-7-aminocephalosporanic acid - ? 115271 3.1.1.41 7-aminocephalosporanic acid + H2O - Bacillus subtilis SHS 0133 acetate + deacetyl-7-aminocephalosporanic acid - ? 115271 3.1.1.41 7-aminocephalosporanic acid + H2O - Thermotoga maritima deacetyl 7-aminocephalosporanic acid + acetate - ? 387647 3.1.1.41 7-aminocephalosporanic acid + H2O - Bacillus subtilis deacetyl 7-aminocephalosporanic acid + acetate reaction product is not less than 97.5% pure, only a negligible amount of deacetyl 7-aminocephalospornolactone detected ? 387647 3.1.1.41 7-aminocephalosporanic acid + H2O - Thermotoga maritima DSM 3109 deacetyl 7-aminocephalosporanic acid + acetate - ? 387647 3.1.1.41 7-aminocephalosporanic acid + H2O - Bacillus subtilis SHS0133 deacetyl 7-aminocephalosporanic acid + acetate reaction product is not less than 97.5% pure, only a negligible amount of deacetyl 7-aminocephalospornolactone detected ? 387647 3.1.1.41 7-aminocephalosporanic acid + H2O - Thermotoga maritima ATCC 43589 deacetyl 7-aminocephalosporanic acid + acetate - ? 387647 3.1.1.41 7-aminocephalosporanic acid + H2O - Thermotoga maritima 7-amino-3-deacetylcephalosporanic acid + acetate - ? 391070 3.1.1.41 7-aminocephalosporanic acid + H2O colorimetric assay method development using bromothymol blue, validation, overview Bacillus pumilus 7-amino-3-deacetylcephalosporanic acid + acetate - ? 391070 3.1.1.41 7-aminocephalosporanic acid + H2O colorimetric assay method development using bromothymol blue, validation, overview Bacillus pumilus CECT 5072 7-amino-3-deacetylcephalosporanic acid + acetate - ? 391070 3.1.1.41 alpha-ketoadipinyl-7-aminocephalosporanic acid + H2O - Escherichia coli 7-aminocephalosporanic acid + alpha-ketoadipate - ? 376237 3.1.1.41 alpha-naphthyl acetate + H2O - Bacillus subtilis 1-naphthol + acetate - ? 73361 3.1.1.41 alpha-naphthyl acetate + H2O - Bacillus subtilis WRRL-B-558 1-naphthol + acetate - ? 73361 3.1.1.41 benzylcephalosporin + H2O i.e. 7-(phenylacetamido)-cephalosporanic acid Fusarium oxysporum acetate + deacetylbenzylcephalosporin - ? 115266 3.1.1.41 benzylcephalosporin + H2O i.e. 7-(phenylacetamido)-cephalosporanic acid Fusarium oxysporum AY F-298 acetate + deacetylbenzylcephalosporin - ? 115266 3.1.1.41 benzyloxycarbonyl-7-aminocephalosporanic acid + H2O - Rhodotorula glutinis ? - ? 115273 3.1.1.41 benzyloxycarbonyl-7-aminocephalosporanic acid + H2O - Rhodotorula glutinis 38B1 ? - ? 115273 3.1.1.41 cephaloglycin + H2O - Bacillus subtilis acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid - ? 115270 3.1.1.41 cephaloglycin + H2O - Bacillus subtilis ATCC 6633 acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid - ? 115270 3.1.1.41 cephaloglycin + H2O - Bacillus subtilis SHS 0133 acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid - ? 115270 3.1.1.41 cephalosporin C + H2O - Bacillus subtilis deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O - Escherichia coli deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O - Rhodotorula glutinis deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O - Staphylococcus sp. deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O - Rhodotorula toruloides deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O - Citrus sp. deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O - Amycolatopsis lactamdurans deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O - Acremonium chrysogenum deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O - Thermotoga maritima deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O last step in the cephalosporin C biosynthetic pathway Rhodotorula toruloides deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O reaction using the recombinant Acremonium chrysogenum strain scaled up to a 4.000 l fermentor Rhodotorula toruloides deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O colorimetric assay method development using bromothymol blue, validation, overview Bacillus pumilus deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O - Amycolatopsis lactamdurans MA4213 deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O - Rhodotorula glutinis 38B1 deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O - Acremonium chrysogenum C10 / ATCC 48272 deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O - Escherichia coli JM105 deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O - Bacillus subtilis SHS0133 deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalosporin C + H2O colorimetric assay method development using bromothymol blue, validation, overview Bacillus pumilus CECT 5072 deacetylcephalosporin C + acetate - ? 115274 3.1.1.41 cephalothin + H2O - Bacillus subtilis acetate + deacetylcephalothin - ? 115268 3.1.1.41 cephalothin + H2O - Citrus sp. acetate + deacetylcephalothin - ? 115268 3.1.1.41 cephalothin + H2O i.e. 7-(thiophene-2-acetamido)-cephalosporanic acid Fusarium oxysporum acetate + deacetylcephalothin - ? 115268 3.1.1.41 cephalothin + H2O - Bacillus subtilis WRRL-B-558 acetate + deacetylcephalothin - ? 115268 3.1.1.41 cephalothin + H2O - Bacillus subtilis ATCC 6633 acetate + deacetylcephalothin - ? 115268 3.1.1.41 cephalothin + H2O i.e. 7-(thiophene-2-acetamido)-cephalosporanic acid Fusarium oxysporum AY F-298 acetate + deacetylcephalothin - ? 115268 3.1.1.41 cephalothin + H2O - Bacillus subtilis SHS 0133 acetate + deacetylcephalothin - ? 115268 3.1.1.41 cephapirin + H2O - Bacillus subtilis acetate + deacetylcephapirin - ? 115269 3.1.1.41 cephapirin + H2O - Bacillus subtilis ATCC 6633 acetate + deacetylcephapirin - ? 115269 3.1.1.41 cephapirin + H2O - Bacillus subtilis SHS 0133 acetate + deacetylcephapirin - ? 115269 3.1.1.41 D-glucose pentaacetate + H2O - Thermotoga maritima D-glucose + acetate - ? 364388 3.1.1.41 D-glucose pentaacetate + H2O - Thermotoga maritima DSM 3109 D-glucose + acetate - ? 364388 3.1.1.41 D-glucose pentaacetate + H2O - Thermotoga maritima ATCC 43589 D-glucose + acetate - ? 364388 3.1.1.41 D-glucose pentaacetate + H2O - Thermotoga maritima ? - ? 381746 3.1.1.41 D-xylose tetraacetate + H2O - Thermotoga maritima ? - ? 381763 3.1.1.41 D-xylose tetraacetate + H2O - Thermotoga maritima D-xylose + acetate - ? 445431 3.1.1.41 deacetyl-7-aminocephalosporanic acid + ethyl acetate - Rhodotorula glutinis 7-aminocephalosporanic acid + ethanol - ? 115283 3.1.1.41 deacetyl-7-aminocephalosporanic acid + ethylene glycol diacetate - Bacillus subtilis 7-aminocephalosporanic acid + ? - ? 115280 3.1.1.41 deacetyl-7-aminocephalosporanic acid + ethylene glycol diacetate - Rhodotorula toruloides 7-aminocephalosporanic acid + ? - ? 115280 3.1.1.41 deacetyl-7-aminocephalosporanic acid + isopropenyl acetate - Bacillus subtilis 7-aminocephalosporanic acid + ? - ? 115281 3.1.1.41 deacetyl-7-aminocephalosporanic acid + methyl acetate - Rhodotorula glutinis 7-aminocephalosporanic acid + methanol - ? 115282 3.1.1.41 ethyl acetate + H2O2 - Bacillus subtilis ethanol + peracetic acid - ? 447504 3.1.1.41 fluorescein di(acetoxymethyl ether) + H2O - Thermotoga maritima ? - ? 445484 3.1.1.41 fluorescein dibutyloxymethyl ether + H2O - Thermotoga maritima ? - ? 445485 3.1.1.41 fluorescein dicaproyloxymethyl ether + H2O - Thermotoga maritima ? - ? 445486 3.1.1.41 fluorescein dipropanoyloxymethyl ether + H2O - Thermotoga maritima ? - ? 445487 3.1.1.41 fluorescein dipropyloxymethyl ether + H2O - Thermotoga maritima ? - ? 447527 3.1.1.41 fluorescein dipropyloxymethyl ether + H2O - Thermotoga maritima DSM 3109 ? - ? 447527 3.1.1.41 fluorescein dipropyloxymethyl ether + H2O - Thermotoga maritima ATCC 43589 ? - ? 447527 3.1.1.41 fluorescein divaleryloxymethyl ether + H2O - Thermotoga maritima ? - ? 445488 3.1.1.41 glucose pentaacetate + H2O - Bacillus subtilis ? - ? 36071 3.1.1.41 glucose pentaacetate + H2O - Bacillus subtilis WRRL-B-558 ? - ? 36071 3.1.1.41 glucose pentaacetate + H2O - Thermotoga maritima ? + acetate - ? 447561 3.1.1.41 glucose pentacetate + H2O - Bacillus subtilis ? - ? 430229 3.1.1.41 glutaryl-7-aminocephalosporanic acid + H2O - Pseudomonas sp. 7-aminocephalosporanic acid + glutamate - ? 376893 3.1.1.41 glutaryl-7-aminocephalosporanic acid + H2O - Pseudomonas sp. GK-16 7-aminocephalosporanic acid + glutamate - ? 376893 3.1.1.41 glycerol triacetate + H2O2 - Bacillus subtilis glycerol diacetate + peracetic acid - ? 447567 3.1.1.41 monoacetin + H2O - Bacillus subtilis acetate + glycerol - ? 115276 3.1.1.41 monochloroacetyl-7-aminocephalosporanic acid + H2O - Rhodotorula glutinis acetate + monochloroacetyl-deacetyl-7-aminocephalosporanic acid - ? 115272 3.1.1.41 monochloroacetyl-7-aminocephalosporanic acid + H2O - Rhodotorula glutinis 38B1 acetate + monochloroacetyl-deacetyl-7-aminocephalosporanic acid - ? 115272 3.1.1.41 additional information no substrate: cephamycin C Amycolatopsis lactamdurans ? - ? 89 3.1.1.41 additional information addition of glucose, maltose, and sucrose to the culture broth suppresses the enzyme production Acremonium chrysogenum ? - ? 89 3.1.1.41 additional information the enzyme has a double specificity on both the acetylated oligosaccharide, cf. EC 3.1.1.72, and cephalosporin C and 7-aminocephalosporanic acid Bacillus subtilis ? - ? 89 3.1.1.41 additional information the enzyme is active on a variety of acetylated compounds, including cephalosporin C, its esterase activity is confined to short-chain acyl esters of C2-C3 chain length Thermotoga maritima ? - ? 89 3.1.1.41 additional information the enzyme hydrolyzes the ester linkages of the acetyl groups in both the xylose moieties of the acetylated xylan fragments Bacillus subtilis ? - ? 89 3.1.1.41 additional information the enzyme is an acetyl esterase and not an acetyl xylan esterase, that shows no activity with xylan or acetylated xylan Thermotoga maritima ? - ? 89 3.1.1.41 additional information no substrate: cephamycin C Amycolatopsis lactamdurans MA4213 ? - ? 89 3.1.1.41 additional information the enzyme has a double specificity on both the acetylated oligosaccharide, cf. EC 3.1.1.72, and cephalosporin C and 7-aminocephalosporanic acid Bacillus subtilis CICC 20034 ? - ? 89 3.1.1.41 additional information the enzyme hydrolyzes the ester linkages of the acetyl groups in both the xylose moieties of the acetylated xylan fragments Bacillus subtilis CICC 20034 ? - ? 89 3.1.1.41 p-nitrophenyl acetate + H2O - Bacillus subtilis 4-nitrophenol + acetate - ? 74069 3.1.1.41 p-nitrophenyl acetate + H2O - Rhodotorula toruloides 4-nitrophenol + acetate - ? 74069 3.1.1.41 p-nitrophenyl acetate + H2O - Bacillus subtilis ATCC 6633 4-nitrophenol + acetate - ? 74069 3.1.1.41 p-nitrophenyl acetate + H2O - Bacillus subtilis SHS 0133 4-nitrophenol + acetate - ? 74069 3.1.1.41 phenoxymethylcephalosporin + H2O i.e. 7-(phenoxyacetamido)-cephalosporanic acid Fusarium oxysporum acetate + deacetylphenoxycephalosporin - ? 115267 3.1.1.41 phenoxymethylcephalosporin + H2O i.e. 7-(phenoxyacetamido)-cephalosporanic acid Fusarium oxysporum AY F-298 acetate + deacetylphenoxycephalosporin - ? 115267 3.1.1.41 tetraacetylxylose + H2O - Thermotoga maritima ? - ? 448163 3.1.1.41 triacetin + H2O - Bacillus subtilis ? - ? 73241 3.1.1.41 triacetin + H2O - Citrus sp. ? - ? 73241 3.1.1.41 triacetin + H2O - Bacillus subtilis WRRL-B-558 ? - ? 73241 3.1.1.41 xylose tetraacetate + H2O - Thermotoga maritima ? + acetate - ? 448273