3.1.1.23 1(3)-arachidonoylglycerol + H2O - Rattus norvegicus ? + glycerol - ? 364032 3.1.1.23 1(3)-monooleoylglycerol + H2O - Rattus norvegicus oleic acid + glycerol - ? 73180 3.1.1.23 1,2-dicaprin + H2O - Mycobacterium tuberculosis ? - ? 390488 3.1.1.23 1,2-dicaprin + H2O - Mycobacterium tuberculosis H37Rv ? - ? 390488 3.1.1.23 1,3-dihydroxypropan-2-yl 4-pyren-1-ylbutanoate - Mus musculus pyrenylbutanoic acid + glycerol - ? 411135 3.1.1.23 1,3-dihydroxypropan-2-yl 4-pyren-1-ylbutanoate - Rattus norvegicus pyrenylbutanoic acid + glycerol - ? 411135 3.1.1.23 1,3-dihydroxypropan-2-yl 4-pyren-1-ylbutanoate + H2O - Homo sapiens pyrenylbutanoic acid + glycerol - ? 411134 3.1.1.23 1-arachidonoylglycerol + H2O - Mus musculus arachidonic acid + glycerol - ? 380837 3.1.1.23 1-arachidonoylglycerol + H2O - Homo sapiens glycerol + arachidonic acid - ? 429124 3.1.1.23 1-arachidonoylglycerol + H2O best substrate Homo sapiens glycerol + arachidonic acid - ? 429124 3.1.1.23 1-capryloyl-rac-glycerol + H2O - Homo sapiens glycerol + caprylic acid - ? 429586 3.1.1.23 1-decanoyl-rac-glycerol + H2O - Homo sapiens glycerol + decanoic acid - ? 429587 3.1.1.23 1-decanoyl-rac-glycerol + H2O best substrate Homo sapiens glycerol + decanoic acid - ? 429587 3.1.1.23 1-lauroyl-rac-glycerol + H2O - Homo sapiens glycerol + lauric acid - ? 429125 3.1.1.23 1-lauroyl-rac-glycerol + H2O more than 2fold higher activity compared to 1-oleoly-rac-glycerol Bacillus sp. (in: Bacteria) glycerol + lauric acid - ? 429125 3.1.1.23 1-lauroyl-rac-glycerol + H2O more than 2fold higher activity compared to 1-oleoly-rac-glycerol Bacillus sp. (in: Bacteria) H-257 glycerol + lauric acid - ? 429125 3.1.1.23 1-linoleoylglycerol + H2O - Homo sapiens glycerol + linoleic acid - ? 429594 3.1.1.23 1-mono-oleyl-rac-glycerol + H2O - Mycobacterium tuberculosis oleate + glycerol - ? 390524 3.1.1.23 1-monobutyroyl-rac-glycerol + H2O - Mycobacterium tuberculosis butyrate + glycerol - ? 390525 3.1.1.23 1-monocaprylin + H2O - Mortierella alliacea ? - ? 416017 3.1.1.23 1-monocapryloylglycerol + H2O best substrate Bacillus sp. (in: Bacteria) caprylic acid + glycerol - ? 364033 3.1.1.23 1-monocapryloylglycerol + H2O best substrate Bacillus sp. (in: Bacteria) H-257 caprylic acid + glycerol - ? 364033 3.1.1.23 1-monodecanoyl-rac-glycerol + H2O - Mycobacterium tuberculosis decanoate + glycerol - ? 390526 3.1.1.23 1-monolauroyl-rac-glycerol + H2O - Mycobacterium tuberculosis laureate + glycerol - ? 390527 3.1.1.23 1-monolauroylglycerol + H2O best substrate Bacillus sp. (in: Bacteria) lauric acid + glycerol - ? 364019 3.1.1.23 1-monolauroylglycerol + H2O good substrate Bacillus sp. (in: Bacteria) lauric acid + glycerol - ? 364019 3.1.1.23 1-monolauroylglycerol + H2O best substrate Bacillus sp. (in: Bacteria) H-257 lauric acid + glycerol - ? 364019 3.1.1.23 1-monolauroylglycerol + H2O good substrate Bacillus sp. (in: Bacteria) H-257 lauric acid + glycerol - ? 364019 3.1.1.23 1-monolinolein + H2O one of the most preferred substrates Mortierella alliacea ? - ? 416018 3.1.1.23 1-monolinolenin + H2O best substrate Mortierella alliacea ? - ? 416019 3.1.1.23 1-monolinoleoylglycerol + H2O - Pseudomonas sp. linolic acid + glycerol - ? 364039 3.1.1.23 1-monolinoleoylycerol + H2O - Bacillus sp. (in: Bacteria) linoleic acid + glycerol - ? 364035 3.1.1.23 1-monomyristoyl-rac-glycerol + H2O - Mycobacterium tuberculosis myristoate + glycerol - ? 390528 3.1.1.23 1-monomyristoylglycerol + H2O - Rattus norvegicus myristic acid + glycerol - ? 73184 3.1.1.23 1-monomyristoylglycerol + H2O best substrate Pseudomonas sp. myristic acid + glycerol - r 73184 3.1.1.23 1-monomyristoylglycerol + H2O good substrate Bacillus sp. (in: Bacteria) myristic acid + glycerol - ? 73184 3.1.1.23 1-monomyristoylglycerol + H2O best substrate Pseudomonas sp. LP7315 myristic acid + glycerol - r 73184 3.1.1.23 1-monomyristoylglycerol + H2O good substrate Bacillus sp. (in: Bacteria) H-257 myristic acid + glycerol - ? 73184 3.1.1.23 1-monooctanoyl-rac-glycerol + H2O - Mycobacterium tuberculosis octanoate + glycerol - ? 390529 3.1.1.23 1-monoolein + H2O one of the most preferred substrates Mortierella alliacea ? - ? 416020 3.1.1.23 1-monooleoylglycerol + H2O - Mus musculus oleic acid + glycerol - ? 364018 3.1.1.23 1-monooleoylglycerol + H2O - Pseudomonas sp. oleic acid + glycerol - ? 364018 3.1.1.23 1-monooleoylglycerol + H2O - Bacillus sp. (in: Bacteria) oleic acid + glycerol - ? 364018 3.1.1.23 1-monooleoylglycerol + H2O - Pseudomonas sp. LP7315 oleic acid + glycerol - ? 364018 3.1.1.23 1-monooleoylglycerol + H2O - Bacillus sp. (in: Bacteria) H-257 oleic acid + glycerol - ? 364018 3.1.1.23 1-monopalmitin + H2O - Mortierella alliacea ? - ? 416021 3.1.1.23 1-monopalmitoylglycerol + H2O - Rattus norvegicus palmitic acid + glycerol - ? 73181 3.1.1.23 1-monopalmitoylglycerol + H2O - Pseudomonas sp. palmitic acid + glycerol - r 73181 3.1.1.23 1-monopalmitoylglycerol + H2O - Bacillus sp. (in: Bacteria) palmitic acid + glycerol - ? 73181 3.1.1.23 1-monopalmitoylglycerol + H2O - Pseudomonas sp. LP7315 palmitic acid + glycerol - r 73181 3.1.1.23 1-monopalmitoylglycerol + H2O - Bacillus sp. (in: Bacteria) H-257 palmitic acid + glycerol - ? 73181 3.1.1.23 1-monostearoylglycerol + H2O - Bacillus sp. (in: Bacteria) stearic acid + glycerol - ? 364034 3.1.1.23 1-myristoyl-rac-glycerol + H2O - Homo sapiens glycerol + myristic acid - ? 429595 3.1.1.23 1-oleoyl-glycerol + H2O - Mycobacterium tuberculosis oleate + glycerol - ? 446570 3.1.1.23 1-oleoyl-glycerol + H2O - Mycobacterium tuberculosis H37Rv oleate + glycerol - ? 446570 3.1.1.23 1-oleoyl-glycerol + H2O - Mycobacterium tuberculosis ATCC 25618 oleate + glycerol - ? 446570 3.1.1.23 1-oleoyl-rac-glycerol + H2O - Bacillus sp. (in: Bacteria) glycerol + oleic acid - ? 429606 3.1.1.23 1-oleoyl-rac-glycerol + H2O - Bacillus sp. (in: Bacteria) H-257 glycerol + oleic acid - ? 429606 3.1.1.23 1-oleoylglycerol + H2O - Mus musculus oleic acid + glycerol - ? 380859 3.1.1.23 1-palmitoyl-2-lysophosphatidylcholine + H2O - Homo sapiens palmitic acid + glycerophosphorylcholine - ? 73178 3.1.1.23 2-(15-deoxy-DELTA12,14-prostaglandin J2)-glycerol + H2O - Homo sapiens ? - ? 429663 3.1.1.23 2-(15-deoxy-DELTA12,14-prostaglandin J2)-glycerol + H2O highest preference Homo sapiens ? - ? 429663 3.1.1.23 2-arachidonoylglycerol + H2O - Mus musculus arachidonic acid + glycerol - ? 364031 3.1.1.23 2-arachidonoylglycerol + H2O - Homo sapiens arachidonic acid + glycerol - ? 364031 3.1.1.23 2-arachidonoylglycerol + H2O - Rattus norvegicus arachidonic acid + glycerol - ? 364031 3.1.1.23 2-arachidonoylglycerol + H2O 2-arachidonoylglycerol is an endocannabinoid which prevents the propagation of harmful neuroinflammation and may function as a gliotransmitter Mus musculus arachidonic acid + glycerol - ? 364031 3.1.1.23 2-arachidonoylglycerol + H2O the enzyme is responsible for degradation of 2-arachidonoylglycerol in HeLa cells Homo sapiens arachidonic acid + glycerol - ? 364031 3.1.1.23 2-arachidonoylglycerol + H2O the enzyme is responsible for degradation of 2-arachidonoylglycerol in the brain Rattus norvegicus arachidonic acid + glycerol - ? 364031 3.1.1.23 2-arachidonoylglycerol + H2O MGL modulates endocannabinoid signaling in vivo by inactivating 2-arachidonoylglycerol (2-AG), the main endogenous agonist for central CB1 and peripheral CB2 cannabinoid receptors Homo sapiens arachidonic acid + glycerol - ? 364031 3.1.1.23 2-arachidonoylglycerol + H2O 2-arachidonoylglycerol is bound in the tetrahedral intermediate state to Ser122 Homo sapiens arachidonic acid + glycerol - ? 364031 3.1.1.23 2-arachidonoylglycerol + H2O the MGL binding site is a single hydrophobic channel, consisting of an L-shaped main channel with a sub-pocket at the turn. The catalytic S122 of MGL is positioned at the bottom of a single channel Homo sapiens arachidonic acid + glycerol - ? 364031 3.1.1.23 2-arachidonoylglycerol + H2O - Rattus norvegicus Sprague-Dawley arachidonic acid + glycerol - ? 364031 3.1.1.23 2-arachidonoylglycerol + H2O - Mus musculus glycerol + arachidonic acid - ? 390127 3.1.1.23 2-arachidonoylglycerol + H2O - Homo sapiens glycerol + arachidonic acid - ? 390127 3.1.1.23 2-arachidonoylglycerol + H2O - Rattus norvegicus glycerol + arachidonic acid - ? 390127 3.1.1.23 2-arachidonoylglycerol + H2O - Bacillus sp. (in: Bacteria) glycerol + arachidonic acid - ? 390127 3.1.1.23 2-arachidonoylglycerol + H2O presynaptic MGL not only hydrolyzes 2-arachidonoylglycerol released from activated postsynaptic neurons but also contributes to degradation of constitutively produced 2-arachidonoylglycerol and prevention of its accumulation around presynaptic terminals. Thus, the MGL activity determines basal endocannabinoid tone and terminates retrograde endocannabinoid signaling in the hippocampus Mus musculus glycerol + arachidonic acid - ? 390127 3.1.1.23 2-arachidonoylglycerol + H2O presynaptic MGL not only hydrolyzes 2-arachidonoylglycerol released from activated postsynaptic neurons but also contributes to degradation of constitutively produced 2-arachidonoylglycerol and prevention of its accumulation around presynaptic terminals. Thus, the MGL activity determines basal endocannabinoid tone and terminates retrograde endocannabinoid signaling in the hippocampus Rattus norvegicus glycerol + arachidonic acid - ? 390127 3.1.1.23 2-arachidonoylglycerol + H2O more than 80% of 2-arachidonoylglycerol (0.05 mM) is hydrolyzed after 90 min in the presence of 0.02 mg of MAGL(+/+) brain extracts at room temperature Mus musculus glycerol + arachidonic acid - ? 390127 3.1.1.23 2-arachidonoylglycerol + H2O - Mus musculus C57BL/6 glycerol + arachidonic acid - ? 390127 3.1.1.23 2-arachidonoylglycerol + H2O presynaptic MGL not only hydrolyzes 2-arachidonoylglycerol released from activated postsynaptic neurons but also contributes to degradation of constitutively produced 2-arachidonoylglycerol and prevention of its accumulation around presynaptic terminals. Thus, the MGL activity determines basal endocannabinoid tone and terminates retrograde endocannabinoid signaling in the hippocampus Mus musculus C57BL/6 glycerol + arachidonic acid - ? 390127 3.1.1.23 2-arachidonoylglycerol + H2O - Bacillus sp. (in: Bacteria) H-257 glycerol + arachidonic acid - ? 390127 3.1.1.23 2-arachidonoylglycerol + H2O - Homo sapiens arachidonoate + glycerol - ? 446030 3.1.1.23 2-arachidonoylglycerol + H2O - Mus musculus arachidonoate + glycerol - ? 446030 3.1.1.23 2-arachidonoylglycerol + H2O - Mus musculus C57BL/6N arachidonoate + glycerol - ? 446030 3.1.1.23 2-linoleoylglycerol + H2O - Homo sapiens glycerol + linoleic acid - ? 429679 3.1.1.23 2-monolinolein + H2O - Mortierella alliacea ? - ? 416148 3.1.1.23 2-monomyristoylglycerol + H2O - Rattus norvegicus myristic acid + glycerol - ? 73185 3.1.1.23 2-monoolein + H2O - Homo sapiens ? - ? 73243 3.1.1.23 2-monoolein + H2O - Rattus norvegicus ? - ? 73243 3.1.1.23 2-monoolein + H2O - Mortierella alliacea ? - ? 73243 3.1.1.23 2-monooleoylglycerol + H2O - Rattus norvegicus oleic acid + glycerol - ? 73179 3.1.1.23 2-monooleoylglycerol + H2O - Rattus norvegicus oleic acid + glycerol - r 73179 3.1.1.23 2-monooleoylglycerol + H2O - Saccharomyces cerevisiae oleic acid + glycerol - ? 73179 3.1.1.23 2-monooleoylglycerol + H2O - Bacillus sp. (in: Bacteria) oleic acid + glycerol - ? 73179 3.1.1.23 2-monooleoylglycerol + H2O - Bacillus sp. (in: Bacteria) H-257 oleic acid + glycerol - ? 73179 3.1.1.23 2-monopalmitoylglycerol + H2O - Rattus norvegicus palmitic acid + glycerol - ? 73182 3.1.1.23 2-oleoylglycerol + H2O - Mus musculus oleic acid + glycerol - ? 380448 3.1.1.23 2-oleoylglycerol + H2O - Homo sapiens oleic acid + glycerol - ? 380448 3.1.1.23 2-oleoylglycerol + H2O - Rattus norvegicus oleic acid + glycerol - ? 380448 3.1.1.23 2-oleoylglycerol + H2O Cys208 plays an important role in MGL function Rattus norvegicus oleic acid + glycerol - ? 380448 3.1.1.23 2-oleoylglycerol + H2O - Rattus norvegicus Sprague-Dawley oleic acid + glycerol - ? 380448 3.1.1.23 2-oleoylglycerol + H2O - Homo sapiens glycerol + oleic acid - ? 390661 3.1.1.23 2-palmitoylglycerol + H2O - Homo sapiens glycerol + palmitic acid - ? 429690 3.1.1.23 3-oleoylglycerol + H2O - Mus musculus oleic acid + glycerol - ? 381106 3.1.1.23 4-methylumbelliferyl butyrate + H2O - Homo sapiens 4-methylumbelliferol + butyrate - ? 363849 3.1.1.23 4-nitrophenyl acetate + H2O - Homo sapiens 4-nitrophenol + acetate - ? 12514 3.1.1.23 4-nitrophenyl acetate + H2O - Arabidopsis thaliana 4-nitrophenol + acetate - ? 12514 3.1.1.23 4-nitrophenyl butyrate + H2O - Bacillus sp. (in: Bacteria) 4-nitrophenol + butyric acid - ? 364036 3.1.1.23 4-nitrophenyl caprate + H2O - Bacillus subtilis 4-nitrophenol + capric acid - ? 115381 3.1.1.23 4-nitrophenyl caprylate + H2O - Bacillus sp. (in: Bacteria) 4-nitrophenol + caprylic acid - ? 364037 3.1.1.23 4-nitrophenyl octanoate + H2O - Bacillus subtilis 4-nitrophenol + octanoate - ? 115380 3.1.1.23 4-nitrophenyl-caproate + H2O - Bacillus subtilis 4-nitrophenol + caproic acid - ? 364023 3.1.1.23 4-nitrophenyl-laurate + H2O - Bacillus subtilis 4-nitrophenol + lauric acid - ? 364026 3.1.1.23 4-nitrophenyl-myristate + H2O - Bacillus subtilis 4-nitrophenol + myristic acid - ? 364027 3.1.1.23 4-nitrophenyl-palmitate + H2O - Bacillus subtilis 4-nitrophenol + palmitic acid - ? 364028 3.1.1.23 4-nitrophenyl-stearate + H2O - Bacillus subtilis 4-nitrophenol + stearic acid - ? 364029 3.1.1.23 4-nitrophenyllaurate + H2O - Pseudomonas sp. 4-nitrophenol + lauric acid - ? 364038 3.1.1.23 4-nitrophenyllaurate + H2O - Pseudomonas sp. 4-nitrophenol + lauric acid - r 364038 3.1.1.23 4-nitrophenyllaurate + H2O - Pseudomonas sp. LP7315 4-nitrophenol + lauric acid - r 364038 3.1.1.23 4-nitrophenyllaurate + H2O - Pseudomonas sp. LP7315 4-nitrophenol + lauric acid - ? 364038 3.1.1.23 7-hydroxycoumarinyl arachidonate - Homo sapiens arachidonic acid + 7-hydroxycoumarin - ? 411417 3.1.1.23 7-hydroxycoumarinyl arachidonate + H2O - Homo sapiens 7-hydroxycoumarin + arachidonate - ? 446896 3.1.1.23 7-hydroxycoumarinyl arachidonate + H2O - Arabidopsis thaliana 7-hydroxycoumarin + arachidonate - ? 446896 3.1.1.23 7-hydroxycoumarinyl arachidonate + H2O fluorescence detection using a fluorogenic probe 1,3-dihydroxypropan-2-yl-4-pyren-1-ylbutanoate Homo sapiens 7-hydroxycoumarin + arachidonate - ? 446896 3.1.1.23 7-hydroxyresorufinyl arachidonate + H2O a red fluorogenic substrate, 7-HRA, that is stable in 10% DMSO for at least 36 h at room temperature and for at least 6 months at 4°C, synthesis, overview Homo sapiens 7-hydroxyresorufin + arachidonate - ? 446897 3.1.1.23 arachidonoyl-1-thio-glycerol + H2O - Bacillus sp. (in: Bacteria) ? - ? 429900 3.1.1.23 arachidonoyl-1-thio-glycerol + H2O - Bacillus sp. (in: Bacteria) H-257 ? - ? 429900 3.1.1.23 arachidonoyl-7-hydroxy-6-methoxy-4-methylcoumarin ester + H2O - Homo sapiens arachidonic acid + 7-hydroxy-6-methoxy-4-methylcoumarin - ? 398731 3.1.1.23 bis(monoacylclycerol) phosphate + H2O at pH 5.5 Rattus norvegicus ? - ? 364040 3.1.1.23 diolein + H2O - Mycobacterium tuberculosis ? - ? 73238 3.1.1.23 diolein + H2O - Mycobacterium tuberculosis H37Rv ? - ? 73238 3.1.1.23 ethyl oleate + H2O - Homo sapiens oleic acid + ethanol - ? 73177 3.1.1.23 glycerol + lauric acid 93% conversion after 6 h Penicillium camemberti monolaurin + H2O - r 417049 3.1.1.23 glycerol + myristic acid 60% conversion after 6 h Penicillium camemberti monomyristin + H2O - r 417050 3.1.1.23 glycerol + oleic acid 46% conversion after 6 h Penicillium camemberti monoolein + H2O - r 417051 3.1.1.23 glycerol + palmitic acid 50% conversion after 6 h Penicillium camemberti monopalmitin + H2O - r 417052 3.1.1.23 glycerol + stearic acid 60% conversion after 6 h Penicillium camemberti monostearin + H2O - r 417056 3.1.1.23 lauric acid + glycerol catalyzes the esterification of lauric acid and glycerol in a homogeneous system, minimal amounts of dilaurin are also synthesized Rhizomucor miehei monolauroyl glycerol + H2O - ? 364030 3.1.1.23 lysobisphosphatidic acid + H2O at pH 5.5 Rattus norvegicus ? - ? 364041 3.1.1.23 lysophosphatidylcholine + H2O - Arabidopsis thaliana fatty acid + glycerophosphocholine - ? 447711 3.1.1.23 lysophosphatidylethanolamine + H2O - Arabidopsis thaliana fatty acid + glycerophosphoethanolamine - ? 447712 3.1.1.23 methyl butyrate + H2O - Rattus norvegicus methanol + butanoate - ? 73186 3.1.1.23 methylcaprylate + H2O low activity Mortierella alliacea ? - ? 417299 3.1.1.23 methyloleate + H2O low activity Mortierella alliacea ? - ? 417302 3.1.1.23 monoacylglycerol + H2O - Arabidopsis thaliana fatty acid + glycerol - ? 360684 3.1.1.23 monoacylglycerol + H2O - Rattus norvegicus acylic acid + glycerol - ? 446309 3.1.1.23 monoacylglycerol + H2O - Rattus norvegicus Sprague-Dawley acylic acid + glycerol - ? 446309 3.1.1.23 monoarachidonoylglycerol + H2O - Rattus norvegicus arachidonic acid + glycerol - ? 364042 3.1.1.23 monolinoleoylglycerol + H2O - Rattus norvegicus linoic acid + glycerol - ? 364020 3.1.1.23 monolinoleoylglycerol + H2O - Bacillus sp. (in: Bacteria) linoic acid + glycerol - ? 364020 3.1.1.23 monolinoleoylglycerol + H2O - Bacillus sp. (in: Bacteria) H-257 linoic acid + glycerol - ? 364020 3.1.1.23 monomyristin + H2O - Mycolicibacterium smegmatis glycerol + myristate - ? 417313 3.1.1.23 monomyristin + H2O - Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 glycerol + myristate - ? 417313 3.1.1.23 monomyristoylglycerol + H2O - Bacillus sp. (in: Bacteria) myristic acid + glycerol - ? 364022 3.1.1.23 monoolein + H2O - Mycolicibacterium smegmatis glycerol + oleate - ? 417314 3.1.1.23 monoolein + H2O - Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 glycerol + oleate - ? 417314 3.1.1.23 monooleoylglycerol + H2O - Gallus gallus oleic acid + glycerol - ? 73176 3.1.1.23 monooleoylglycerol + H2O - Rattus norvegicus oleic acid + glycerol - ? 73176 3.1.1.23 monooleoylglycerol + H2O preferred substrate Homo sapiens oleic acid + glycerol - ? 73176 3.1.1.23 monostearoylglycerol + H2O - Rattus norvegicus stearic acid + glycerol - ? 364021 3.1.1.23 monostearoylglycerol + H2O - Bacillus sp. (in: Bacteria) stearic acid + glycerol - ? 364021 3.1.1.23 monostearoylglycerol + H2O - Bacillus sp. (in: Bacteria) H-257 stearic acid + glycerol - ? 364021 3.1.1.23 additional information - Rattus norvegicus ? - ? 89 3.1.1.23 additional information no activity against long-chain diacylglycerols and triacylglycerols, cholesterol ester, or lysophosphatidylcholine Rattus norvegicus ? - ? 89 3.1.1.23 additional information substrate specificity, overview Rattus norvegicus ? - ? 89 3.1.1.23 additional information enzyme also catalyzes monoglyceride synthesis in a solvent-free two-phase system, in which fatty acid droplets are dispersed in the glycerol phase with a low water content, enzyme does not hydrolyze di- and triglycerides Pseudomonas sp. ? - ? 89 3.1.1.23 additional information enzyme does not hydrolyze palmitoylethanolamine, enzyme preferentially hydrolyzes 2-monoglycerides Rattus norvegicus ? - ? 89 3.1.1.23 additional information enzyme exhibits both phosphatidic acid-preferring phospholipase A2 and monoacylglycerol lipase activities with a modest specificity towards unsaturated acyl chains, veryl low di- and triaclyglycerol lipase activity Rattus norvegicus ? - ? 89 3.1.1.23 additional information hydrolyzes monoacylglycerols but not di- or triacylglycerols Bacillus subtilis ? - ? 89 3.1.1.23 additional information preferentially hydrolyzes triacylglycerol-esters and 4-nitrophenyl-esters of fatty acids with short chain lengths up to 10 carbon atoms, no hydrolysis of triolein Bacillus subtilis ? - ? 89 3.1.1.23 additional information catalyzes the last step in the hydrolysis of stored triglycerides in the adipocytes Mus musculus ? - ? 89 3.1.1.23 additional information monoacylglycerol lipase is required in the final hydrolysis of 2-monoacylglycerols produced by hormone-sensitive lipase Rattus norvegicus ? - ? 89 3.1.1.23 additional information inhibition of the enzyme potentiates the activation of 2-arachidonoylglycerol production by ATP Mus musculus ? - ? 89 3.1.1.23 additional information enzyme activity with 1-, 2-, and 3- monoacylglycerols as substrates is similar Mus musculus ? - ? 89 3.1.1.23 additional information enzyme activity with 1-, 2-, and 3-monoacylglycerols as substrates is similar Mus musculus ? - ? 89 3.1.1.23 additional information developmental and nutritional regulation of the enzyme in the intestine, overview Homo sapiens ? - ? 89 3.1.1.23 additional information key enzyme responsible for the termination of endocannabinoid signaling with a crucial role in 2-arachidonoylglycerol metabolism, MAGL is unable to mediate anandamide degradation, overview Homo sapiens ? - ? 89 3.1.1.23 additional information Rv0183, as exported enzyme, may be involved in the degradation of the host cell lipids Mycobacterium tuberculosis ? - ? 89 3.1.1.23 additional information the enzyme is involved in prostaglandin metabolism in brain particulate fractions Rattus norvegicus ? - ? 89 3.1.1.23 additional information tissue-specific developmental and nutritional regulation of the enzyme, post-transcriptional regulation of expression occurs in the intestine, while transcriptional regulation is the primary mechanism for hepatic MGL expression, overview Mus musculus ? - ? 89 3.1.1.23 additional information substrate specificity, the enzyme hydrolyses monoacylglycerol substrates, both long chain di- and triacylglycerols, although the turnover is lower than with monoacylglycerol, overview, no activity of the recombinant enzyme with lysophospholipid substrates, recombinant Rv0183 hydrolyses synthetic vinyl esters with various acyl chain lengths, chain length dependence, overview Mycobacterium tuberculosis ? - ? 89 3.1.1.23 additional information monoacylglycerols containing long-chain unsaturated fatty acids and phosphatidylcholine are preferentially hydrolyzed over triacylglycerols and fatty acid methyl esters. Triacylglycerol may be formed via 2-monoacylglycerol Mortierella alliacea ? - ? 89 3.1.1.23 additional information no activity with triacetin, tributyrin, trioctanoin, triolein, dioctanoin, dimyristin, monooctanoin, diolein, and Tween 80 Mycolicibacterium smegmatis ? - ? 89 3.1.1.23 additional information the enzyme also uses soybean oil, sesame oil, rapeseed oil, camellia oil, olive oil and linseed oil as substrates. 1,3-dicaprylin, 1,3-dipalmitin, 1,2-diolein, 1,3-diolein, 1,2-dilinolein, methylbutyrate, methyllaurate, methylpalmitate, methylpamitoleate, methylsterate, methyllinoleate, methyllinolenate, and methyleicosapentaenoate yield no detectable activity at either 30 or 50°C Mortierella alliacea ? - ? 89 3.1.1.23 additional information direct NMR detection of a reversible equilibrium between active and inactive states of human MGL (hMGL) that is slow on the NMR time scale and can be modulated in a controlled manner by pH, temperature, and select point mutations Homo sapiens ? - ? 89 3.1.1.23 additional information docking studies with the substrate 1-oleoyl-glycerol in the form of the tetrahedral reaction intermediate. In the best-docking mode, the polar glycerol moiety of the substrate is in direct interaction with catalytically important residues Ser110, His256, Glu257, Met111 and Leu39 from the core region and Tyr181 situated within helix 3 of the cap. No positional preference for sn-1(3) or 2-MGs has been reported for any MGL Mycobacterium tuberculosis ? - ? 89 3.1.1.23 additional information human monoacylglycerol lipase (MAGL) displays catalytic activity on monoacyl glycerols with medium to long-chain lipophylic moieties such as stearoyl, palmitoyl, oleoyl, and especially arachidonoyl groups. MAGL also demonstrates preference for the hydrolysis of 2-acyl glycerols over their 1(3)-regioisomers. Measurement of MAGL-catalyzed hydrolysis of p-nitrophenyl alkyl esters by monitoring the liberation of p-nitrophenol. Fluorogenic enzyme assay method evaluation, overview Homo sapiens ? - ? 89 3.1.1.23 additional information usage of a substrate with linoleic acid at the sn-1 position for enzyme activity assays Homo sapiens ? - ? 89 3.1.1.23 additional information usage of a substrate with linoleic acid at the sn-1 position for enzyme activity assays. The Arabidopsis thaliana isozyme MAGL16 (AtMAGL16) shows very weak lipase activity. AtMAGL16 may not be involved in the hydrolysis of lipid substrates Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of a substrate with linoleic acid at the sn-1 position for enzyme activity assays. The Arabidopsis thaliana isozyme MAGL6 (AtMAGL6) shows strong lipase activity Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of a substrate with linoleic acid at the sn-1 position for enzyme activity assays. The Arabidopsis thaliana isozyme MAGL8 (AtMAGL8) shows strong lipase activity Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of various lipid substrates containing 18:2 fatty acids at the sn-1 (monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol), sn-2 (triacylglycerol and digalactosyldiacylglycerol), and sn-3 (diacylglycerol, triacylglycerol) positions. Isozyme AtMAGL1 is active with monoacylglycerol and lysophosphatidylethanolamine substrates, but not with diacylglycerol, triacylglycerol, and lysophosphatidylcholine substrates. Isozyme regiospecificities and substrate specificities, specificities for monoacylglycerols with various fatty acids at sn-1 position, overview Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of various lipid substrates containing 18:2 fatty acids at the sn-1 (monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol), sn-2 (triacylglycerol and digalactosyldiacylglycerol), and sn-3 (diacylglycerol, triacylglycerol) positions. Isozyme AtMAGL10 is active with monoacylglycerol substrates, but not with diacylglycerol, triacylglycerol, lysophosphatidylethanolamine, and lysophosphatidylcholine substrates. Isozyme regiospecificities and substrate specificities, specificities for monoacylglycerols with various fatty acids at sn-1 position, overview Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of various lipid substrates containing 18:2 fatty acids at the sn-1 (monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol), sn-2 (triacylglycerol and digalactosyldiacylglycerol), and sn-3 (diacylglycerol, triacylglycerol) positions. Isozyme AtMAGL11 is inactive with monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, and lysophosphatidylethanolamine substrates. Isozyme regiospecificities and substrate specificities, specificities for monoacylglycerols with various fatty acids at sn-1 position, overview Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of various lipid substrates containing 18:2 fatty acids at the sn-1 (monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol), sn-2 (triacylglycerol and digalactosyldiacylglycerol), and sn-3 (diacylglycerol, triacylglycerol) positions. Isozyme AtMAGL14 is active with monoacylglycerol substrates, but not with diacylglycerol, triacylglycerol, lysophosphatidylethanolamine, and lysophosphatidylcholine substrates. Isozymes regiospecificities and substrate specificities, specificities for monoacylglycerols with various fatty acids at sn-1 position, overview Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of various lipid substrates containing 18:2 fatty acids at the sn-1 (monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol), sn-2 (triacylglycerol and digalactosyldiacylglycerol), and sn-3 (diacylglycerol, triacylglycerol) positions. Isozyme AtMAGL15 is active with monoacylglycerol substrates, but not with diacylglycerol, triacylglycerol, lysophosphatidylethanolamine, and lysophosphatidylcholine substrates. Isozymes regiospecificities and substrate specificities, specificities for monoacylglycerols with various fatty acids at sn-1 position, overview Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of various lipid substrates containing 18:2 fatty acids at the sn-1 (monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol), sn-2 (triacylglycerol and digalactosyldiacylglycerol), and sn-3 (diacylglycerol, triacylglycerol) positions. Isozyme AtMAGL16 is active with monoacylglycerol substrates, but not with diacylglycerol, triacylglycerol, lysophosphatidylethanolamine, and lysophosphatidylcholine substrates. Isozymes regiospecificities and substrate specificities, specificities for monoacylglycerols with various fatty acids at sn-1 position, overview Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of various lipid substrates containing 18:2 fatty acids at the sn-1 (monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol), sn-2 (triacylglycerol and digalactosyldiacylglycerol), and sn-3 (diacylglycerol, triacylglycerol) positions. Isozyme AtMAGL2 is active with monoacylglycerol and lysophosphatidylethanolamine substrates, but not with diacylglycerol, triacylglycerol, lysophosphatidylcholine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol substrates. Isozyme regiospecificities and substrate specificities, specificities for monoacylglycerols with various fatty acids at sn-1 position, overview Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of various lipid substrates containing 18:2 fatty acids at the sn-1 (monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol), sn-2 (triacylglycerol and digalactosyldiacylglycerol), and sn-3 (diacylglycerol, triacylglycerol) positions. Isozyme AtMAGL3 is inactive with monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, and lysophosphatidylethanolamine substrates. Isozyme regiospecificities and substrate specificities, specificities for monoacylglycerols with various fatty acids at sn-1 position, overview Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of various lipid substrates containing 18:2 fatty acids at the sn-1 (monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol), sn-2 (triacylglycerol and digalactosyldiacylglycerol), and sn-3 (diacylglycerol, triacylglycerol) positions. Isozyme AtMAGL4 is active with monoacylglycerol, lysophosphatidylcholine, and lysophosphatidylethanolamine substrates, but not with diacylglycerol, and triacylglycerol substrates as well as with monogalactosyldiacylglycerol and digalactosyldiacylglycerol substrates. Isozyme regiospecificities and substrate specificities, specificities for monoacylglycerols with various fatty acids at sn-1 position, overview Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of various lipid substrates containing 18:2 fatty acids at the sn-1 (monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol), sn-2 (triacylglycerol and digalactosyldiacylglycerol), and sn-3 (diacylglycerol, triacylglycerol) positions. Isozyme AtMAGL5 is inactive with monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol substrates. Isozyme regiospecificities and substrate specificities, specificities for monoacylglycerols with various fatty acids at sn-1 position, overview Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of various lipid substrates containing 18:2 fatty acids at the sn-1 (monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol), sn-2 (triacylglycerol and digalactosyldiacylglycerol), and sn-3 (diacylglycerol, triacylglycerol) positions. Isozyme AtMAGL6 is active with monoacylglycerol substrates, but not with diacylglycerol, triacylglycerol, lysophosphatidylethanolamine, and lysophosphatidylcholine substrates. Isozyme regiospecificities and substrate specificities, specificities for monoacylglycerols with various fatty acids at sn-1 position, overview Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of various lipid substrates containing 18:2 fatty acids at the sn-1 (monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol), sn-2 (triacylglycerol and digalactosyldiacylglycerol), and sn-3 (diacylglycerol, triacylglycerol) positions. Isozyme AtMAGL7 is active with monoacylglycerol substrates, but not with diacylglycerol, triacylglycerol, lysophosphatidylethanolamine, and lysophosphatidylcholine substrates. Isozyme regiospecificities and substrate specificities, specificities for monoacylglycerols with various fatty acids at sn-1 position, overview Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of various lipid substrates containing 18:2 fatty acids at the sn-1 (monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol), sn-2 (triacylglycerol and digalactosyldiacylglycerol), and sn-3 (diacylglycerol, triacylglycerol) positions. Isozyme AtMAGL8 is active with monoacylglycerol substrates, but not with diacylglycerol, triacylglycerol, lysophosphatidylethanolamine, and lysophosphatidylcholine substrates. Isozyme regiospecificities and substrate specificities, specificities for monoacylglycerols with various fatty acids at sn-1 position, overview Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information usage of various lipid substrates containing 18:2 fatty acids at the sn-1 (monoacylglycerol, diacylglycerol, triacylglycerol, lysophosphatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol), sn-2 (triacylglycerol and digalactosyldiacylglycerol), and sn-3 (diacylglycerol, triacylglycerol) positions. Isozyme AtMAGL9 is active with monoacylglycerol substrates, but not with diacylglycerol, triacylglycerol, lysophosphatidylethanolamine, and lysophosphatidylcholine substrates. Isozyme regiospecificities and substrate specificities, specificities for monoacylglycerols with various fatty acids at sn-1 position, overview Arabidopsis thaliana ? - ? 89 3.1.1.23 additional information no activity with triacetin, tributyrin, trioctanoin, triolein, dioctanoin, dimyristin, monooctanoin, diolein, and Tween 80 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ? - ? 89 3.1.1.23 additional information hydrolyzes monoacylglycerols but not di- or triacylglycerols Bacillus subtilis H-257 ? - ? 89 3.1.1.23 additional information enzyme also catalyzes monoglyceride synthesis in a solvent-free two-phase system, in which fatty acid droplets are dispersed in the glycerol phase with a low water content, enzyme does not hydrolyze di- and triglycerides Pseudomonas sp. LP7315 ? - ? 89 3.1.1.23 additional information tissue-specific developmental and nutritional regulation of the enzyme, post-transcriptional regulation of expression occurs in the intestine, while transcriptional regulation is the primary mechanism for hepatic MGL expression, overview Mus musculus C57BL/6 ? - ? 89 3.1.1.23 additional information Rv0183, as exported enzyme, may be involved in the degradation of the host cell lipids Mycobacterium tuberculosis H37Rv ? - ? 89 3.1.1.23 additional information substrate specificity, the enzyme hydrolyses monoacylglycerol substrates, both long chain di- and triacylglycerols, although the turnover is lower than with monoacylglycerol, overview, no activity of the recombinant enzyme with lysophospholipid substrates, recombinant Rv0183 hydrolyses synthetic vinyl esters with various acyl chain lengths, chain length dependence, overview Mycobacterium tuberculosis H37Rv ? - ? 89 3.1.1.23 additional information docking studies with the substrate 1-oleoyl-glycerol in the form of the tetrahedral reaction intermediate. In the best-docking mode, the polar glycerol moiety of the substrate is in direct interaction with catalytically important residues Ser110, His256, Glu257, Met111 and Leu39 from the core region and Tyr181 situated within helix 3 of the cap. No positional preference for sn-1(3) or 2-MGs has been reported for any MGL Mycobacterium tuberculosis H37Rv ? - ? 89 3.1.1.23 additional information docking studies with the substrate 1-oleoyl-glycerol in the form of the tetrahedral reaction intermediate. In the best-docking mode, the polar glycerol moiety of the substrate is in direct interaction with catalytically important residues Ser110, His256, Glu257, Met111 and Leu39 from the core region and Tyr181 situated within helix 3 of the cap. No positional preference for sn-1(3) or 2-MGs has been reported for any MGL Mycobacterium tuberculosis ATCC 25618 ? - ? 89 3.1.1.23 additional information monoacylglycerols containing long-chain unsaturated fatty acids and phosphatidylcholine are preferentially hydrolyzed over triacylglycerols and fatty acid methyl esters. Triacylglycerol may be formed via 2-monoacylglycerol Mortierella alliacea YN-15 ? - ? 89 3.1.1.23 additional information the enzyme also uses soybean oil, sesame oil, rapeseed oil, camellia oil, olive oil and linseed oil as substrates. 1,3-dicaprylin, 1,3-dipalmitin, 1,2-diolein, 1,3-diolein, 1,2-dilinolein, methylbutyrate, methyllaurate, methylpalmitate, methylpamitoleate, methylsterate, methyllinoleate, methyllinolenate, and methyleicosapentaenoate yield no detectable activity at either 30 or 50°C Mortierella alliacea YN-15 ? - ? 89 3.1.1.23 palmitoyl-CoA + H2O - Rattus norvegicus palmitic acid + CoA - ? 73183 3.1.1.23 phosphatidylcholine + H2O one of the most preferred substrates Mortierella alliacea ? - ? 64439 3.1.1.23 phosphatidylcholine + H2O one of the most preferred substrates Mortierella alliacea YN-15 ? - ? 64439 3.1.1.23 prostaglandin D2-G + H2O - Rattus norvegicus ? - ? 393355 3.1.1.23 prostaglandin D2-glycerol + H2O - Homo sapiens ? - ? 430655 3.1.1.23 prostaglandin E2-G + H2O - Rattus norvegicus ? - ? 393356 3.1.1.23 prostaglandin E2-glycerol + H2O - Homo sapiens ? - ? 430656 3.1.1.23 prostaglandin F2alpha-glycerol + H2O - Homo sapiens ? - ? 430657 3.1.1.23 prostaglandin F3alpha-G + H2O - Rattus norvegicus ? - ? 393357 3.1.1.23 rac-1(3)-oleoylglycerol + H2O - Saccharomyces cerevisiae oleate + glycerol - ? 417562 3.1.1.23 sn-2-arachidonoylglycerol + H2O - Rattus norvegicus arachidonic acid + glycerol - ? 73174 3.1.1.23 sn-2-arachidonoylglycerol + H2O - Bos taurus arachidonic acid + glycerol - ? 73174 3.1.1.23 sn-2-monoolein + H2O - Mus musculus glycerol + oleic acid - ? 393510 3.1.1.23 sn-2-monoolein + H2O - Homo sapiens glycerol + oleic acid - ? 393510 3.1.1.23 sn-2-monoolein + H2O - Mus musculus C57BL/6 glycerol + oleic acid - ? 393510 3.1.1.23 sn-2-palmitoylglycerol + H2O - Bos taurus palmitic acid + glycerol - ? 73175 3.1.1.23 soybean oil + H2O - Mycobacterium tuberculosis ? - ? 364064 3.1.1.23 soybean oil + H2O - Mycobacterium tuberculosis H37Rv ? - ? 364064 3.1.1.23 tributyrylglycerol + H2O - Rattus norvegicus ? - ? 73187 3.1.1.23 tricaprylin + H2O - Mortierella alliacea ? - ? 64400 3.1.1.23 tricaprylin + H2O - Mortierella alliacea YN-15 ? - ? 64400 3.1.1.23 trieicosapentaenoin + H2O low activity Mortierella alliacea ? - ? 417717 3.1.1.23 trilaurin + H2O low activity Mortierella alliacea ? - ? 417719 3.1.1.23 trilinolein + H2O low activity Mortierella alliacea ? - ? 417720 3.1.1.23 trilinolenin + H2O low activity Mortierella alliacea ? - ? 417721 3.1.1.23 triolein + H2O the lipase strongly hydrolyzes the sn-1/3 ester bonds and weakly hydrolyzes the sn-2 ester bonds of triolein Mortierella alliacea ? - ? 64454 3.1.1.23 triolein + H2O the lipase strongly hydrolyzes the sn-1/3 ester bonds and weakly hydrolyzes the sn-2 ester bonds of triolein Mortierella alliacea YN-15 ? - ? 64454 3.1.1.23 tripalmitin + H2O - Mortierella alliacea ? - ? 413001 3.1.1.23 tripalmitolein + H2O - Mortierella alliacea ? - ? 417725 3.1.1.23 tristearin + H2O - Mortierella alliacea ? - ? 413004 3.1.1.23 umbelliferyl arachidonate + H2O - Homo sapiens umbelliferol + arachidonic acid - ? 417772