2.8.1.8	biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-octanoyl)Lys-Ala-Ala-Ser-As + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] ferredoxin	-	Thermus thermophilus	biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-lipoyl)Lys-Ala-Ala-Ser-As + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] ferredoxin	-	?	437226	
2.8.1.8	biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-octanoyl)Lys-Ala-Ala-Ser-As + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] ferredoxin	-	Escherichia coli	biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-lipoyl)Lys-Ala-Ala-Ser-As + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] ferredoxin	-	?	437226	
2.8.1.8	Glu-Ser-Val-(N6-octanoyl)Lys-Ala-Ala-Ser-Asp + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite	-	Thermus thermophilus	Glu-Ser-Val-(N6-lipoyl)Lys-Ala-Ala-Ser-Asp + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite	-	?	437575	
2.8.1.8	Glu-Ser-Val-(N6-octanoyl)Lys-Ala-Ala-Ser-Asp + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite	-	Escherichia coli	Glu-Ser-Val-(N6-lipoyl)Lys-Ala-Ala-Ser-Asp + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite	-	?	437575	
2.8.1.8	H protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] dithionite	-	Mycobacterium tuberculosis	H protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] dithionite	-	?	437598	
2.8.1.8	H protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] flavodoxin	-	Mycobacterium tuberculosis	H protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] flavodoxin	-	?	437599	
2.8.1.8	additional information	final step in de novo biosynthesis of lipoyl cofactor. The lipoyl cofactor is essential for the function of pyruvate dehydrogenase (E2 domain)	Escherichia coli	?	-	?	89	
2.8.1.8	additional information	final step in de novo biosynthesis of lipoyl cofactor. The lipoyl cofactor is essential for the function of pyruvate dehydrogenase (E2 domain)	Neurospora crassa	?	-	?	89	
2.8.1.8	additional information	final step in de novo biosynthesis of lipoyl cofactor. The lipoyl cofactor is essential for the function of pyruvate dehydrogenase (E2 domain)	Pisum sativum	?	-	?	89	
2.8.1.8	additional information	insertion of sulfur into octanoyl groups is first at C6 to form an enzyme bound intermediate, and in a subsequent step a second sulfur is inserted at C8	Saccharolobus solfataricus	?	-	?	89	
2.8.1.8	protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine	both sulfur atoms in lipoic acid are contributed by the same lipoyl synthase polypeptide	Escherichia coli	protein N6-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosyl radicals	-	?	380225	
2.8.1.8	protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine	octanoylated pyruvate dehydrogenase E2 domain	Escherichia coli	protein N6-(lipoyl)lysine + 2 L-methionine + 5'-deoxyadenosyl radical	-	?	380226	
2.8.1.8	protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine	lipoyl-bearing subunit of the glycine cleavage system (H-protein) is a substrate for LipA. 5'-deoxyadenosyl radical acts directly on the octanoyl substrate. 2 equivalents of S-adenosyl-L-methionine are cleaved irreversibly in forming 1 equivalent of [lipoyl]H-protein and are consistent with a model in which two LipA proteins are required to synthesize one lipoyl group	Escherichia coli	protein N6-(lipoyl)lysine + L-methionine + 5'-deoxyadenosyl radicals	-	ir	380227	
2.8.1.8	protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine	tetrapeptide substrate, containing an Nepsilon-octanoyl lysine residue, corresponding in sequence to the lipoyl binding domain of the E2 subunit of pyruvate dehydrogenase	Saccharolobus solfataricus	protein N6-(lipoyl)lysine + L-methionine + 5'-deoxyadenosyl radicals	-	?	380227	
2.8.1.8	protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine	-	Bacillus subtilis	protein N6-(lipoyl)lysine + L-methionine + 5'-deoxyadenosine	-	?	424972	
2.8.1.8	protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine	-	Bacillus subtilis	protein N6-(lipoyl)lysine + L-methionine + 5'-deoxyadenosine	-	ir	424972	
2.8.1.8	protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine	-	Arabidopsis thaliana	protein N6-(lipoyl)lysine + L-methionine + 5'-deoxyadenosine	-	?	424972	
2.8.1.8	protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] ferredoxin	-	Mycobacterium tuberculosis	protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] ferredoxin	-	?	436548	
2.8.1.8	protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] cluster	-	Thermus thermophilus	protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] cluster	-	?	436549	
2.8.1.8	protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] cluster	-	Escherichia coli	protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] cluster	-	?	436549	
2.8.1.8	[8,8,8-2H3]-octanoyl-H protein + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite	-	Thermus thermophilus	[8,8,8-2H3]-lipoyl-H protein + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite	-	?	437990	
2.8.1.8	[8,8,8-2H3]-octanoyl-H protein + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite	-	Escherichia coli	[8,8,8-2H3]-lipoyl-H protein + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite	-	?	437990	
2.8.1.8	[protein]-N6-(octanoyl)-L-lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+	substrate is a chemically synthesized octanoyl-octapeptide whose sequence corresponds to that of the lipoyl domain in the H-protein of Thermococcus kodakarensis	Thermococcus kodakarensis	[protein]-N6-[(R)-dihydrolipoyl]-L-lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin	-	?	460027	
2.8.1.8	[protein]-N6-(octanoyl)-L-lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+	substrate is a chemically synthesized octanoyl-octapeptide whose sequence corresponds to that of the lipoyl domain in the H-protein of Thermococcus kodakarensis	Thermococcus kodakarensis ATCC BAA-918	[protein]-N6-[(R)-dihydrolipoyl]-L-lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin	-	?	460027