2.7.9.3 2'(3')-O-(2,4,6-trinitrophenyl)adenosine-5'-triphosphate + selenide + H2O the compound is used as a synthetic analogue of the substrate ATP for the monitoring and quantitative analysis of the functional activity of SPS Escherichia coli 2'(3')-O-(2,4,6-trinitrophenyl)adenosine-5'-monophosphate + selenophosphate + phosphate - ? 436730 2.7.9.3 2'(3')-O-(2,4,6-trinitrophenyl)adenosine-5'-triphosphate + selenide + H2O the compound is used as a synthetic analogue of the substrate ATP for the monitoring and quantitative analysis of the functional activity of SPS Escherichia coli DH5alpha-T1 and BL21-Gold(DE3) 2'(3')-O-(2,4,6-trinitrophenyl)adenosine-5'-monophosphate + selenophosphate + phosphate - ? 436730 2.7.9.3 ATP + dithiothreitol + H2O as effective as selenide Salmonella enterica subsp. enterica serovar Typhimurium ? - ? 359996 2.7.9.3 ATP + selenide - Escherichia coli AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide - Bos taurus AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide - Trypanosoma brucei AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide - Caenorhabditis elegans AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide - Mus musculus AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide - Leishmania major AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide - Drosophila melanogaster AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide - Enterococcus faecalis AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide SelD is a key factor for Se utilization by generating a Se donor compound and therefore discriminating Se from sulfur for further use Enterococcus faecalis AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide selenophosphate synthetase catalyzes the activation of selenide with ATP to synthesize selenophosphate, the reactive selenium donor for biosyntheses of both the 21st amino acid selenocysteine and 2-selenouridine nucleotides in tRNA anticodons Aquifex aeolicus AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide selenophosphate synthetase produces the biologically active selenium donor compound, monoselenophosphate, from ATP and selenide, for the synthesis of selenocysteine Trypanosoma brucei AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide selenophosphate synthetase produces the biologically active selenium donor compound, monoselenophosphate, from ATP and selenide, for the synthesis of selenocysteine Leishmania major AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide SPS2 Mus musculus AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide SPS2 acts as an autoregulator of selenoprotein synthesis, selenocysteine biosynthesis, overview Mus musculus AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide SPS2, the catalytically important selenocysteine residue is located at position 63 Mus musculus AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide the phosphate moiety of selenophosphate is derived from the ATP gamma-phosphate, while the orthophosphate is from the beta-phosphate Aquifex aeolicus AMP + selenophosphate + phosphate - ? 398795 2.7.9.3 ATP + selenide + H2O - Salmonella enterica subsp. enterica serovar Typhimurium AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Drosophila melanogaster AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Haemophilus influenzae AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Mus musculus AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Escherichia coli AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Escherichia coli AMP + selenophosphate + phosphate - r 359995 2.7.9.3 ATP + selenide + H2O - Homo sapiens AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Rattus norvegicus AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Bos taurus AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Clostridioides difficile AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Methanococcus vannielii AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Leishmania major AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Penaeus monodon AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Methanococcus maripaludis AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Trypanosoma brucei brucei AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Caenorhabditis elegans AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O specific for ATP Mus musculus AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O specific for ATP Escherichia coli AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O selenide can be substituted by dithiothreitol, but only very poorly by L-selenocysteine and NaSH Salmonella enterica subsp. enterica serovar Typhimurium AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O in the absence of selenide, ATP is converted completely to AMP and phosphate Escherichia coli AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O in the absence of selenide, ATP is converted completely to AMP and phosphate Escherichia coli AMP + selenophosphate + phosphate selenophosphate is derived from the gamma phosphoryl group and phosphate from the beta phosphoryl group of ATP ? 359995 2.7.9.3 ATP + selenide + H2O the gamma-phosphoryl group of the substrate ATP is cleaved in a kinetically competent reaction to form a phosphoryl-enzyme intermediate in the absence of the second substrate, selenide Escherichia coli AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O provides the selenium donor substrate for the rRNA 2-selenouridine synthase reaction Salmonella enterica subsp. enterica serovar Typhimurium AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O the enzyme provides the biologically active selenium donor compound monoseleniumphosphate Escherichia coli AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O required for synthesis of selenocysteine and seleno-tRNAs Homo sapiens AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O required for synthesis of selenocysteine and seleno-tRNAs Mus musculus AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O involved in synthesis of selenoproteins, that provide protection against oxidative stress by acting as cellular antioxidants Drosophila melanogaster AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O required for synthesis of selenoproteins and therefore involved in cell differentiation and development Drosophila melanogaster AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O provides selenophosphate, that can serve as selenium donor for the reaction in which 5-methylaminomethyl-2-thiouridine is converted to 5-methylaminomethyl-2-selenouridine Salmonella enterica subsp. enterica serovar Typhimurium AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O the Sps1-encoded enzyme depends on a selenium salvage system that recycles L-selenocysteine Homo sapiens AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O the Sps2-encoded can function with a selenite assimilation system Homo sapiens AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O Sec replaces the catalytically essential Cys residue, SPS is strictly conserved in the organisms that utilize Sec and/or selenouridine Aquifex aeolicus AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O the phosphate moiety of selenophosphate is derived from the ATP gamma-phosphate group, while the orthophosphate is from the beta-phosphate group. Although Thr221 is not essential for the reaction, it contributes to the enzyme activity, probably by interacting with the ATP gamma-phosphate group and a water molecule. Sec/Cys13 and Lys16 are essential for the SPS catalysis. Thr221 and Gly222 interact with the gamma-phosphate group of ATP Aquifex aeolicus AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O the enzyme is involved in biosynthesis of selenophosphate, that is the in vivo selenium donor for selenoprotein synthesis of Methanococcus maripaludis S2 Methanococcus maripaludis AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Mus musculus C57BL/6 AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Clostridioides difficile R20291 AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Escherichia coli DH5alpha-T1 and BL21-Gold(DE3) AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 ATP + selenide + H2O - Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 AMP + selenophosphate + phosphate - ? 359995 2.7.9.3 additional information a DNA replication-related element downstream from the initiation site of selenophosphate synthetase 2 gene is essential for its transcription Drosophila melanogaster ? - ? 89 2.7.9.3 additional information Enterococcus faecalis lacks both the selenocysteine and selenouridine systems, but possesses orphan selenophosphate synthetase SelD Enterococcus faecalis ? - ? 89 2.7.9.3 additional information enzymes involved in selenium utilization, i.e. SelA/SelB and YbbB that define Sec and SeU pathways, respectively, and NADH oxidoreductase generating a SelD substrate, overview. Non-Sec utilization of selenium occurs in Enterococcus faecalis, metabolic labeling, overview Enterococcus faecalis ? - ? 89 2.7.9.3 additional information Haloarcula marismortui lacks both the selenocysteine and selenouridine systems, but possesses orphan selenophosphate synthetase SelD Haloarcula marismortui ? - ? 89 2.7.9.3 additional information SPS orthologs, SPS1 and SPS2, exist, and SPS2 is the one that synthesizes selenophosphate, while SPS1 does not Aquifex aeolicus ? - ? 89 2.7.9.3 additional information SPS1 probably has a specialized, non-essential role in selenoprotein metabolism Mus musculus ? - ? 89 2.7.9.3 additional information Sps2 is both a selenoprotein and a factor involved in synthesis of other selenoproteins Mus musculus ? - ? 89 2.7.9.3 additional information SPS2 is essential for generating the selenium donor for selenocysteine biosynthesis in mammals Mus musculus ? - ? 89 2.7.9.3 additional information SPS2 is involved in the synthesis of selenophosphate for Sel synthesis, selen and selenoprotien metabolism, physiological functions, detailed overview Homo sapiens ? - ? 89 2.7.9.3 additional information no activity by selenophosphate synthetase 1 Escherichia coli ? - ? 89 2.7.9.3 additional information no activity by selenophosphate synthetase 1 Mus musculus ? - ? 89 2.7.9.3 additional information no activity by selenophosphate synthetase 1 Drosophila melanogaster ? - ? 89 2.7.9.3 additional information Se in the form of the amino acid, selenocysteine, is incorporated into selenoproteins at UGA codons Mus musculus ? - ? 89 2.7.9.3 additional information SPS1 shows no activity Mus musculus ? - ? 89 2.7.9.3 additional information development of a non-radioactive assay for detection of selenophosphate synthetase activity using recombinant pyruvate diphosphate dikinase, PPDK, from Thermus thermophilus strain HB8 in an enzyme-coupled reaction system, overview Homo sapiens ? - ? 89