2.7.8.11 1-stearoyl-2-arachidonoylphosphatidylinositol + CMP - Mus musculus CDP-1-stearoyl-2-arachidonoylglycerol + myo-inositol - r 359924 2.7.8.11 2-deoxy-CDP-diacylglycerol + myo-inositol - Homo sapiens CMP + ? - r 359915 2.7.8.11 2-deoxy-CDP-diacylglycerol + myo-inositol optimal activity is 25% higher then with CDP-diacylglycerol Homo sapiens CMP + ? - r 359915 2.7.8.11 2-deoxy-CDP-diacylglycerol + myo-inositol maximal activity only 36% of the optimal activity reached with CDP-diacylglycerol Candida albicans CMP + ? - r 359915 2.7.8.11 CDP-1-arachidonoyl-2-stearoylglycerol + myo-inositol 6% of the activity with CDP-1-stearoyl-2-oleoylglycerol Cavia porcellus CMP + 1-arachidonoyl-2-stearoylphosphtidylinositol - r 359925 2.7.8.11 CDP-1-stearoyl-2-arachidonoylglycerol + myo-inositol 4% of the activity with CDP-1-stearoyl-2-oleoylglycerol Cavia porcellus CMP + 1-stearoyl-2-arachidonoylphosphatidylinositol - r 359923 2.7.8.11 CDP-1-stearoyl-2-oleoylglycerol + myo-inositol - Cavia porcellus CMP + 1-stearoyl-2-oleoylphosphatidylinositol - r 359919 2.7.8.11 CDP-diacylglycerol + 1D-chiro-inositol conversion of 30.9% CDP-diacylglycerol (utilization significant compared to background, p: 0.05), microsomal fraction, 30°C, 20 min Tetrahymena vorax CMP + phosphatidyl-1D-chiro-inositol - ? 400628 2.7.8.11 CDP-diacylglycerol + 1L-chiro-inositol conversion of 25.1% CDP-diacylglycerol (non-significant), microsomal fraction, 30°C, 20 min Tetrahymena vorax CMP + phosphatidyl-1L-chiro-inositol - ? 400629 2.7.8.11 CDP-diacylglycerol + allo-inositol 83% of the activity on myo-inositol Tetrahymena vorax CMP + phosphatidyl-allo-inositol - ? 388298 2.7.8.11 CDP-diacylglycerol + allo-inositol conversion of 23.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30°C, 20 min Tetrahymena vorax CMP + phosphatidyl-allo-inositol - ? 388298 2.7.8.11 CDP-diacylglycerol + allo-inositol 83% of the activity on myo-inositol Tetrahymena vorax V2S CMP + phosphatidyl-allo-inositol - ? 388298 2.7.8.11 CDP-diacylglycerol + allo-inositol conversion of 23.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30°C, 20 min Tetrahymena vorax V2S CMP + phosphatidyl-allo-inositol - ? 388298 2.7.8.11 CDP-diacylglycerol + D-chiro-inositol 82% of the activity on myo-inositol Tetrahymena vorax CMP + phosphatidyl-chiro-inositol - ? 388299 2.7.8.11 CDP-diacylglycerol + D-myo-inositol key step in lipid biosynthesis Arabidopsis thaliana CMP + phosphatidyl-1D-myo-inositol - ? 398830 2.7.8.11 CDP-diacylglycerol + D-myo-inositol D-myo-inositol substrates: phosphatidylethanolamine, phosphatidic acid, overexpressed enzyme in bacterial membrane pellets, 30 min, 22°C, pH 8, 1% (v/v) Triton X-100, 20 mM MgCl2 Arabidopsis thaliana CMP + phosphatidyl-1D-myo-inositol fatty acid analysis by thin-layer chromatography ? 398830 2.7.8.11 CDP-diacylglycerol + epi-inositol 62% of the activity on myo-inositol Tetrahymena vorax CMP + phosphatidyl-epi-inositol - ? 388300 2.7.8.11 CDP-diacylglycerol + epi-inositol conversion of 31.1% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, 30°C, 20 min Tetrahymena vorax CMP + phosphatidyl-epi-inositol - ? 388300 2.7.8.11 CDP-diacylglycerol + L-chiro-inositol 67% of the activity on myo-inositol Tetrahymena vorax CMP + phosphatidyl-chiro-inositol - ? 388301 2.7.8.11 CDP-diacylglycerol + muco-inositol 41% of the activity on myo-inositol Tetrahymena vorax CMP + phosphatidyl-muco-inositol - ? 388302 2.7.8.11 CDP-diacylglycerol + muco-inositol conversion of 15.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30°C, 20 min Tetrahymena vorax CMP + phosphatidyl-muco-inositol - ? 388302 2.7.8.11 CDP-diacylglycerol + myo-inositol - Gallus gallus CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Meleagris gallopavo CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Cavia porcellus CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Chlamydomonas reinhardtii CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Mus musculus CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Homo sapiens CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Rattus norvegicus CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Saccharomyces cerevisiae CMP + phosphatidyl-1D-myo-inositol - ? 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Saccharomyces cerevisiae CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Bos taurus CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Oryctolagus cuniculus CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Zea mays CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Glycine max CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Arabidopsis thaliana CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Plasmodium knowlesi CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Canis lupus familiaris CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Mycobacterium tuberculosis CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Ricinus communis CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Candida albicans CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Toxoplasma gondii CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol formation of membrane phospholipids essential for the growth Saccharomyces cerevisiae CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol Rattus norvegicus CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol Bos taurus CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol Zea mays CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol Glycine max CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol Canis lupus familiaris CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol Ricinus communis CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol key enzyme in cell membrane biosynthesis Homo sapiens CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol membrane lipid biosynthesis Chlamydomonas reinhardtii CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol enzyme plays a role in resynthesis of phosphatidylinositol during agonist-stimulated inositol-phospholipid metabolism Canis lupus familiaris CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol phosphatidylinositol synthase activity increased when zinc is depleted from the growth medium. Analysis of a zrt1DELTA zrt2DELTA mutant defective in plasma membrane zinc transport indicates that the cytoplasmic levels of zinc are responsible for the regulation of phosphatidylinositol synthase. Regulation of PIS1 gene expression by zinc depletion is mediated by the zinc-regulated transcription factor Zap1p. Direct in-teraction between glutathione S-transferase (GST)-Zap1p 687–880 and a putative upstream activating sequence (UAS) zinc-responsive element in the PIS1 Saccharomyces cerevisiae CMP + phosphatidyl-1D-myo-inositol - ? 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol high specificityfor myo-inositol Trypanosoma brucei CMP + phosphatidyl-1D-myo-inositol - ? 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol - Toxoplasma gondii 76K CMP + phosphatidyl-1D-myo-inositol - r 359914 2.7.8.11 CDP-diacylglycerol + myo-inositol myo-inositol and scyllo-inositol are preferred substrates Tetrahymena vorax CMP + phosphatidyl-myo-inositol - ? 388303 2.7.8.11 CDP-diacylglycerol + myo-inositol conversion of 37.4% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, standard reaction: 20 min, 30°C, pH 8, 2 mM MgCl2, 2 mM MnCl2, 1.6 mM Triton X-100 Tetrahymena vorax CMP + phosphatidyl-myo-inositol analyses by liquid scintillation counting and thin-layer chromatography ? 388303 2.7.8.11 CDP-diacylglycerol + myo-inositol parasite membrane extracts, infected red blood cells Plasmodium falciparum CMP + phosphatidyl-myo-inositol - ? 388303 2.7.8.11 CDP-diacylglycerol + myo-inositol - Homo sapiens CMP + 1-phosphatidyl-1D-myo-inositol - ? 436422 2.7.8.11 CDP-diacylglycerol + myo-inositol - Bos taurus CMP + 1-phosphatidyl-1D-myo-inositol - ? 436422 2.7.8.11 CDP-diacylglycerol + myo-inositol - Saccharomyces cerevisiae CMP + 1-phosphatidyl-1D-myo-inositol - ? 436422 2.7.8.11 CDP-diacylglycerol + myo-inositol - Zea mays CMP + 1-phosphatidyl-1D-myo-inositol - ? 436422 2.7.8.11 CDP-diacylglycerol + myo-inositol specificity of PI synthase for the substrates, no activity with 1L-myo-inositol 1-phosphate Homo sapiens CMP + 1-phosphatidyl-1D-myo-inositol - ? 436422 2.7.8.11 CDP-diacylglycerol + myo-inositol specificity of PI synthase for the substrates, no activity with 1L-myo-inositol 1-phosphate Saccharomyces cerevisiae CMP + 1-phosphatidyl-1D-myo-inositol - ? 436422 2.7.8.11 CDP-diacylglycerol + myo-inositol - Zea mays DH4866 CMP + 1-phosphatidyl-1D-myo-inositol - ? 436422 2.7.8.11 CDP-diacylglycerol + myo-inositol - Saccharomyces cerevisiae BY4742 CMP + 1-phosphatidyl-1D-myo-inositol - ? 436422 2.7.8.11 CDP-diacylglycerol + scyllo-inositol myo-inositol and scyllo-inositol are preferred substrates Tetrahymena vorax CMP + phosphatidyl-scyllo-inositol - ? 388304 2.7.8.11 CDP-diacylglycerol + scyllo-inositol conversion of 33.7% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, 30°C, 20 min Tetrahymena vorax CMP + phosphatidyl-scyllo-inositol - ? 388304 2.7.8.11 CDP-diarachidonoylglycerol + myo-inositol 9% of the activity with CDP-1-stearoyl-2-oleoylglycerol Cavia porcellus CMP + diarachidonoylphosphatidylinositol - r 359922 2.7.8.11 CDP-didecanoyl-sn-glycerol + myo-inositol preferred substrate Bos taurus CMP + didecanoylphosphatidylinositol - r 359917 2.7.8.11 CDP-diheptadecanoylglycerol + D-myo-inositol - Arabidopsis thaliana CMP + 1,2-diheptadecanoyl-phosphatidyl-1D-myo-inositol - ? 400630 2.7.8.11 CDP-dilinoleoylglycerol + D-myo-inositol - Arabidopsis thaliana CMP + 1,2-dilinoleoyl-phosphatidyl-1D-myo-inositol - ? 400631 2.7.8.11 CDP-dilinoleoylglycerol + D-myo-inositol highest activity, preferred in competition assay additionally containing 1,2-dipalmitoyl-, 1,2-dioleoyl-, and 1,2-diheptadecanoyl-CDP-diacylglycerol Arabidopsis thaliana CMP + 1,2-dilinoleoyl-phosphatidyl-1D-myo-inositol - ? 400631 2.7.8.11 CDP-dioleoylglycerol + D-myo-inositol - Arabidopsis thaliana CMP + 1,2-dioleoyl-phosphatidyl-1D-myo-inositol - ? 400632 2.7.8.11 CDP-dioleoylglycerol + D-myo-inositol highest activity, preferred in competition assay additionally containing 1,2-dipalmitoyl-, 1,2-dilinoleoyl-, and 1,2-diheptadecanoyl-CDP-diacylglycerol Arabidopsis thaliana CMP + 1,2-dioleoyl-phosphatidyl-1D-myo-inositol - ? 400632 2.7.8.11 CDP-dioleoylglycerol + myo-inositol - Bos taurus CMP + dioleoylphosphatidylinositol - r 359916 2.7.8.11 CDP-dioleoylglycerol + myo-inositol - Oryctolagus cuniculus CMP + dioleoylphosphatidylinositol - r 359916 2.7.8.11 CDP-dipalmitin + myo-inositol - Canis lupus familiaris ? - ? 452442 2.7.8.11 CDP-dipalmitoyl-sn-glycerol + myo-inositol - Cavia porcellus CMP + dipalmitoylphosphatidylinositol - ? 359918 2.7.8.11 CDP-dipalmitoyl-sn-glycerol + myo-inositol - Bos taurus CMP + dipalmitoylphosphatidylinositol - r 359918 2.7.8.11 CDP-dipalmitoylglyceride + myo-inositol - Ricinus communis ? - ? 452443 2.7.8.11 CDP-dipalmitoylglycerol + D-myo-inositol - Arabidopsis thaliana CMP + 1,2-dipalmitoyl-phosphatidyl-1D-myo-inositol - ? 400633 2.7.8.11 CDP-distearoylglycerol + myo-inositol 38% of the activity with CDP-1-stearoyl-2-oleoylglycerol Cavia porcellus CMP + distearoylphosphatidylinositol - r 359921 2.7.8.11 CDPdipalmitoyl-sn-glycerol + myo-inositol 70% of the activity with CDP-1-stearoyl-2-oleoylglycerol Cavia porcellus CMP + dipalmitoylphosphatidylinositol - r 359920 2.7.8.11 additional information - Saccharomyces cerevisiae ? - ? 89 2.7.8.11 additional information CDP, CTP, UMP, AMP, and GMP, are no substrates Mus musculus ? - ? 89 2.7.8.11 additional information UDP-diacylglycerol, ADP-diacylglycerol, and GDP-diacylglycerol are inactive as substrates Homo sapiens ? - ? 89 2.7.8.11 additional information enzyme also catalyzes diacylglycerol-independent exchange reaction between phosphatidylinositol and inositol Chlamydomonas reinhardtii ? - ? 89 2.7.8.11 additional information enzyme also catalyzes diacylglycerol-independent exchange reaction between phosphatidylinositol and inositol Saccharomyces cerevisiae ? - ? 89 2.7.8.11 additional information enzyme also catalyzes diacylglycerol-independent exchange reaction between phosphatidylinositol and inositol Arabidopsis thaliana ? - ? 89 2.7.8.11 additional information enzyme also catalyzes diacylglycerol-independent exchange reaction between phosphatidylinositol and inositol Ricinus communis ? - ? 89 2.7.8.11 additional information formation of novel non-myo-inositol-containing second messengers, preferred non-myo-inositols are scyllo- and chiro-inositol Tetrahymena vorax ? - ? 89 2.7.8.11 additional information highly efficient conversion of biological mixture of CDP-diacylglycerol substrates (synthesised from phosphatidylethanolamine in plant leaves) into phosphatidyl-1D-myo-inositol mainly containing palmitic, acid linoleic acid and linolenic acid, complete conversion of CDP-diacylglycerol residues containing palmitic, acid linoleic acid and linolenic acid Arabidopsis thaliana ? - ? 89 2.7.8.11 additional information in transgenic plants, elevated formation of phosphatidic acid, phosphatidylinositol, and polyphosphoinositide (PtdIns(4,5)P, PtdIns(4,5)P2) that are enriched in polyunsaturated fatty acids compared to wild-type plants Arabidopsis thaliana ? - ? 89 2.7.8.11 additional information in transgenic plants, elevated formation of phosphatidic acid, phosphatidylinositol, phosphatidylethanolamine and diacylglycerol containing less polyunsaturated fatty acids but more saturated and monounsaturated fatty acids, no change in fatty acid composition of polyphopshoinositides compared to wild-type plants Arabidopsis thaliana ? - ? 89 2.7.8.11 additional information microsomal fraction contains additional PdtIn: inositol phosphatidyl transferase activity (inositol headgroup exchange, 20 min, 30°C) between myo-, scyllo-, 1D-chiro-inositol and phosphatidyl-myo-inositol, increased in presence of CMP, least specificity for 1L-chiro- and muco-inositol Tetrahymena vorax ? - ? 89 2.7.8.11 additional information moderate conversion of biological mixture of CDP-diacylglycerol substrates (synthesised from phosphatidylethanolamine in plant leaves) into phosphatidyl-1D-myo-inositol mainly containing palmitic acid and little linoleic acid, non-converted CDP-diacylglycerol residues containing linoleic acid and linolenic acid Arabidopsis thaliana ? - ? 89 2.7.8.11 additional information myo- and scyllo-inositol preferred inositol isomers for PtdIn synthase activity, least specificity for muco-inositol Tetrahymena vorax ? - ? 89 2.7.8.11 additional information partial complementation of PIS activity by overexpression of PfPIS/ScPIS hybrids in ScPIS1-deficient Saccharomyces cerevisiae mutant strain BY4743, analysis by enzyme assay (30°C, yeast membrane pellet, in presence of 18 mM octyl-beta-D-glucopyranoside) or lipid incorporation and extraction Plasmodium falciparum ? - ? 89 2.7.8.11 additional information preference of C18-fatty acid CDP-diacylglycerol substrates Arabidopsis thaliana ? - ? 89 2.7.8.11 additional information preference of polyansaturated C18-fatty acid CDP-diacylglycerol substrates Arabidopsis thaliana ? - ? 89 2.7.8.11 additional information cytosolically made inositol and CDP-diacylglycerol can access the active site of the yeast PI synthase Pis1 from the cytosolic side Saccharomyces cerevisiae ? - ? 89 2.7.8.11 additional information cytosolically made inositol and CDP-diacylglycerol can access the active site of the yeast PI synthase Pis1 from the cytosolic side Saccharomyces cerevisiae BY4742 ? - ? 89 2.7.8.11 additional information formation of novel non-myo-inositol-containing second messengers, preferred non-myo-inositols are scyllo- and chiro-inositol Tetrahymena vorax V2S ? - ? 89 2.7.8.11 additional information microsomal fraction contains additional PdtIn: inositol phosphatidyl transferase activity (inositol headgroup exchange, 20 min, 30°C) between myo-, scyllo-, 1D-chiro-inositol and phosphatidyl-myo-inositol, increased in presence of CMP, least specificity for 1L-chiro- and muco-inositol Tetrahymena vorax V2S ? - ? 89 2.7.8.11 additional information myo- and scyllo-inositol preferred inositol isomers for PtdIn synthase activity, least specificity for muco-inositol Tetrahymena vorax V2S ? - ? 89