2.7.7.B5	ATP + TPHE39A	truncated tRNA consisting of 39 nucleotides derived from tRNAPhe, minimal RNA substrate for modification by ThiI. The crystal structure of TPHE39A shows that base pairs in the T-stem are almost completely disrupted, although those in the acceptor-stem are preserved. ThiI can efficiently bind with not only tRNAPhe but also TPHE39A	Escherichia coli	411694	
2.7.7.B5	additional information	ThiI is involved in the modification of uridine to 4-thiouridine	Escherichia coli	89	
2.7.7.B5	additional information	ThiI plays a role in the transfer of sulfur from cysteine to both thiamine and 4-thiouridine	Escherichia coli	89	
2.7.7.B5	tRNA-uridine + ATP	-	Escherichia coli	402056	
2.7.7.B5	tRNA-uridine + ATP	based upon sequence alignments, ThiI shares a unique P-loop motif with the PPi synthetase family. ThiI very likely catalyzes the adenylation of a substrate, probably a molecule bearing a carbonyl oxygen	Escherichia coli	402056	
2.7.7.B5	tRNA-uridine + GTP	kcat/Km for GTP is about 6fold lower than the value for ATP	Escherichia coli	402057