2.7.4.14 ATP + (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine Cidofovir Homo sapiens ADP + (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine - ? 387898 2.7.4.14 ATP + 1-beta-D-arabinofuranosylcytosine - Homo sapiens ADP + ? - ? 359778 2.7.4.14 ATP + 2',2'-difluorodeoxycytidine - Homo sapiens ADP + ? - ? 359779 2.7.4.14 ATP + 2'-azido-CMP 4fold higher activity with 2'-azido-dCMP than with 2'-azido-CMP Homo sapiens ADP + 2'-azido-CDP - ? 400017 2.7.4.14 ATP + 2'-azido-D-dCMP - Homo sapiens ADP + 2'-azido-D-dCDP - ? 452353 2.7.4.14 ATP + 2'-azido-D-dUMP - Homo sapiens ADP + 2'-azido-D-dUDP - ? 452354 2.7.4.14 ATP + 2'-azido-dCMP 4fold higher activity with 2'-azido-dCMP than with 2'-azido-CMP Homo sapiens ADP + 2'-azido-dCDP - ? 400018 2.7.4.14 ATP + 2'-azido-dUMP similar activity with 2'-azido-UMP and 2'-azido-dUMP Homo sapiens ADP + 2'-azido-dUDP - ? 400019 2.7.4.14 ATP + 2'-azido-L-dCMP - Homo sapiens ADP + 2'-azido-L-dCDP - ? 452355 2.7.4.14 ATP + 2'-azido-L-dUMP - Homo sapiens ADP + 2'-azido-L-dUDP - ? 452356 2.7.4.14 ATP + 2'-azido-UMP similar activity with 2'-azido-UMP and 2'-azido-dUMP Homo sapiens ADP + 2'-azido-UDP - ? 400020 2.7.4.14 ATP + 5-aza-cidofovir - Homo sapiens ADP + phospho-5-aza-cidofovir - ? 436306 2.7.4.14 ATP + 5-aza-cidofovir wild-type enzyme Homo sapiens ADP + phospho-5-aza-cidofovir - ? 436306 2.7.4.14 ATP + AMP - Homo sapiens ADP + ADP - r 357335 2.7.4.14 ATP + ara-CMP - Homo sapiens ADP + ara-CDP - ? 359747 2.7.4.14 ATP + ara-CMP - Dictyostelium sp. ADP + ara-CDP - ? 359747 2.7.4.14 ATP + beta-D-2',3'-dideoxy-CMP + H2O - Homo sapiens ADP + ? - ? 359752 2.7.4.14 ATP + beta-D-2'-azido-2'-dCMP - Homo sapiens ADP + beta-D-2'-azido-2'-dCDP - ? 387974 2.7.4.14 ATP + beta-D-2'-azido-2'-dUMP - Homo sapiens ADP + beta-D-2'-azido-2'-dUDP - ? 387975 2.7.4.14 ATP + beta-L-2',3'-dideoxy-2',3'-didehydro-5-fluoro-CMP - Homo sapiens ADP + ? - ? 359755 2.7.4.14 ATP + beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate - Homo sapiens ADP + beta-L-2',3'-dideoxy-3'-thiacytidine diphosphate - ? 359753 2.7.4.14 ATP + beta-L-2',3'-dideoxy-CMP + H2O - Homo sapiens ADP + ? - ? 359754 2.7.4.14 ATP + beta-L-2'-azido-2'-dCMP - Homo sapiens ADP + beta-L-2'-azido-2'-dCDP - ? 387976 2.7.4.14 ATP + beta-L-2'-azido-2'-dUMP - Homo sapiens ADP + beta-L-2'-azido-2'-dUDP - ? 387977 2.7.4.14 ATP + beta-L-dioxolane-cytidine - Homo sapiens ADP + ? - ? 359751 2.7.4.14 ATP + cCMP - Dictyostelium sp. ? - ? 452352 2.7.4.14 ATP + cidofovir i.e. (S)-[1-(4-amino-2-oxo-pyrimidin-1-yl)-3-hydroxy-propan-2-yl]oxy-methylphosphonic acid Homo sapiens ADP + phospho-cidofovir - ? 436323 2.7.4.14 ATP + cidofovir i.e. (S)-[1-(4-amino-2-oxo-pyrimidin-1-yl)-3-hydroxy-propan-2-yl]oxy-methylphosphonic acid, wild-type enzyme Homo sapiens ADP + phospho-cidofovir - ? 436323 2.7.4.14 ATP + CMP - Drosophila melanogaster ADP + CDP - ? 359742 2.7.4.14 ATP + CMP - Escherichia coli ADP + CDP - ? 359742 2.7.4.14 ATP + CMP - Homo sapiens ADP + CDP - ? 359742 2.7.4.14 ATP + CMP - Homo sapiens ADP + CDP - r 359742 2.7.4.14 ATP + CMP - Rattus norvegicus ADP + CDP - ? 359742 2.7.4.14 ATP + CMP - Sus scrofa ADP + CDP - ? 359742 2.7.4.14 ATP + CMP - Saccharomyces cerevisiae ADP + CDP - ? 359742 2.7.4.14 ATP + CMP - Tetrahymena pyriformis ADP + CDP - ? 359742 2.7.4.14 ATP + CMP - Arabidopsis thaliana ADP + CDP - ? 359742 2.7.4.14 ATP + CMP - Dictyostelium discoideum ADP + CDP - ? 359742 2.7.4.14 ATP + CMP - Mycoplasma mycoides ADP + CDP - ? 359742 2.7.4.14 ATP + CMP - Dictyostelium sp. ADP + CDP - ? 359742 2.7.4.14 ATP + CMP low activity Homo sapiens ADP + CDP - ? 359742 2.7.4.14 ATP + CMP preferred substrate Homo sapiens ADP + CDP - ? 359742 2.7.4.14 ATP + CMP the enzyme is required during rapid cell growth Homo sapiens ADP + CDP - ? 359742 2.7.4.14 ATP + CMP the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis Homo sapiens ADP + CDP - ? 359742 2.7.4.14 ATP + CMP the enzyme catalyses an important step in the phosphorylation of UTP, CTP and dCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies Homo sapiens ADP + CDP - ? 359742 2.7.4.14 ATP + CMP wild-type enzyme and mutant P64T Homo sapiens ADP + CDP - ? 359742 2.7.4.14 ATP + CMP - Escherichia coli B / ATCC 11303 ADP + CDP - ? 359742 2.7.4.14 ATP + cytarabine anticancer drug, phosphorylation catalyzed by the enzyme in cidofovir-resistant HaCaT cells Homo sapiens ADP + phospho-cytarabine - ? 436328 2.7.4.14 ATP + cytarabine i.e. araC, wild-type enzyme and mutant P64T Homo sapiens ADP + phospho-cytarabine - ? 436328 2.7.4.14 ATP + D-CMP - Homo sapiens ADP + D-CDP - ? 388000 2.7.4.14 ATP + D-dCMP - Homo sapiens ADP + D-dCDP - ? 388002 2.7.4.14 ATP + D-dUMP - Homo sapiens ADP + D-dUDP - ? 388003 2.7.4.14 ATP + D-UMP - Homo sapiens ADP + D-UDP - ? 388024 2.7.4.14 ATP + dAMP - Homo sapiens ADP + dADP - ? 358196 2.7.4.14 ATP + dCMP - Drosophila melanogaster ADP + dCDP - ? 358199 2.7.4.14 ATP + dCMP - Homo sapiens ADP + dCDP - ? 358199 2.7.4.14 ATP + dCMP - Rattus norvegicus ADP + dCDP - ? 358199 2.7.4.14 ATP + dCMP - Saccharomyces cerevisiae ADP + dCDP - ? 358199 2.7.4.14 ATP + dCMP - Tetrahymena pyriformis ADP + dCDP - ? 358199 2.7.4.14 ATP + dCMP high activity Homo sapiens ADP + dCDP - ? 358199 2.7.4.14 ATP + dCMP no evidence for reversal of the reaction Mycoplasma mycoides ADP + dCDP - ? 358199 2.7.4.14 ATP + dCMP no evidence for reversal of the reaction Mycoplasma mycoides ADP + dCDP - ir 358199 2.7.4.14 ATP + dCMP the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis Homo sapiens ADP + dCDP - ? 358199 2.7.4.14 ATP + dCMP the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies Homo sapiens ADP + dCDP - ? 358199 2.7.4.14 ATP + dCMP higher activity with the D-enantiomer compared to the L-enantiomer Homo sapiens ADP + dCDP - ? 358199 2.7.4.14 ATP + dCMP wild-type enzyme and mutant P64T Homo sapiens ADP + dCDP - ? 358199 2.7.4.14 ATP + dUMP - Drosophila melanogaster ADP + dUDP - ? 359746 2.7.4.14 ATP + dUMP - Homo sapiens ADP + dUDP - ? 359746 2.7.4.14 ATP + dUMP - Saccharomyces cerevisiae ADP + dUDP - ? 359746 2.7.4.14 ATP + dUMP best substrate Homo sapiens ADP + dUDP - ? 359746 2.7.4.14 ATP + dUMP wild-type enzyme Homo sapiens ADP + dUDP - ? 359746 2.7.4.14 ATP + dUMP no activity with CDP, dTMP, AMP, GMP Homo sapiens ADP + dUDP - ? 359746 2.7.4.14 ATP + dUMP higher activity with the D-enantiomer compared to the L-enantiomer Homo sapiens ADP + dUDP - ? 359746 2.7.4.14 ATP + gemcitabine activity of DCK Homo sapiens ADP + gemcitabine monophosphate i.e. 2',2'-difluorodeoxycytidine 5'-monophosphate ? 400155 2.7.4.14 ATP + gemcitabine i.e. 2',2'-difluorodeoxycytidine Homo sapiens ADP + phospho-gemcitabine - ? 422934 2.7.4.14 ATP + gemcitabine i.e. 2',2'-difluorodeoxycytidine, wild-type enzyme Homo sapiens ADP + phospho-gemcitabine - ? 422934 2.7.4.14 ATP + gemcitabine monophosphate - Homo sapiens ADP + ? - ? 359748 2.7.4.14 ATP + gemcitabine phosphate activity of CMPK Homo sapiens ADP + gemcitabine diphosphate i.e. 2',2'-difluorodeoxycytidine 5'-diphosphate ? 400154 2.7.4.14 ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP - Homo sapiens ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP - ? 359749 2.7.4.14 ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP - Dictyostelium sp. ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP - ? 359749 2.7.4.14 ATP + L-(-)-2',3'-dideoxy-5-fluoro-3'-thia-CMP - Homo sapiens ADP + ? - ? 359750 2.7.4.14 ATP + L-3TCMP - Homo sapiens ADP + L-3TCMP - ? 388071 2.7.4.14 ATP + L-CMP - Homo sapiens ADP + L-CDP - ? 388075 2.7.4.14 ATP + L-CMP poor substrate Homo sapiens ADP + L-CDP - ? 388075 2.7.4.14 ATP + L-dCMP - Homo sapiens ADP + L-dCDP - ? 388076 2.7.4.14 ATP + L-dCMP slower phosphorylation than the D-enantiomers Homo sapiens ADP + L-dCDP - ? 388076 2.7.4.14 ATP + L-dUMP - Homo sapiens ADP + L-dUDP - ? 388077 2.7.4.14 ATP + L-dUMP slower phosphorylation than the D-enantiomers Homo sapiens ADP + L-dUDP - ? 388077 2.7.4.14 ATP + UMP - Drosophila melanogaster ADP + UDP - ? 358236 2.7.4.14 ATP + UMP - Escherichia coli ADP + UDP - ? 358236 2.7.4.14 ATP + UMP - Homo sapiens ADP + UDP - ? 358236 2.7.4.14 ATP + UMP - Homo sapiens ADP + UDP - r 358236 2.7.4.14 ATP + UMP - Rattus norvegicus ADP + UDP - ? 358236 2.7.4.14 ATP + UMP - Sus scrofa ADP + UDP - ? 358236 2.7.4.14 ATP + UMP - Saccharomyces cerevisiae ADP + UDP - ? 358236 2.7.4.14 ATP + UMP - Tetrahymena pyriformis ADP + UDP - ? 358236 2.7.4.14 ATP + UMP - Arabidopsis thaliana ADP + UDP - ? 358236 2.7.4.14 ATP + UMP - Dictyostelium sp. ADP + UDP - ? 358236 2.7.4.14 ATP + UMP low activity Homo sapiens ADP + UDP - ? 358236 2.7.4.14 ATP + UMP preferred substrate Homo sapiens ADP + UDP - ? 358236 2.7.4.14 ATP + UMP the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis Homo sapiens ADP + UDP - ? 358236 2.7.4.14 ATP + UMP the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies Homo sapiens ADP + UDP - ? 358236 2.7.4.14 ATP + UMP wild-type enzyme and mutant P64T Homo sapiens ADP + UDP - ? 358236 2.7.4.14 ATP + UMP - Escherichia coli B / ATCC 11303 ADP + UDP - ? 358236 2.7.4.14 CTP + CMP - Homo sapiens CDP + CDP - ? 359774 2.7.4.14 CTP + CMP - Rattus norvegicus CDP + CDP - ? 359774 2.7.4.14 dATP + CMP - Homo sapiens dADP + CDP - ? 359758 2.7.4.14 dATP + CMP - Rattus norvegicus dADP + CDP - ? 359758 2.7.4.14 dATP + dCMP - Rattus norvegicus dADP + dCDP - ? 359757 2.7.4.14 dATP + UMP - Homo sapiens dADP + ADP - ? 359759 2.7.4.14 dATP + UMP - Homo sapiens dADP + ADP - r 359759 2.7.4.14 dATP + UMP - Rattus norvegicus dADP + ADP - ? 359759 2.7.4.14 dATP + UMP dATP shows 10% of the activity with ATP Tetrahymena pyriformis dADP + ADP - ? 359759 2.7.4.14 dCTP + CMP - Rattus norvegicus dCDP + CDP - ? 359763 2.7.4.14 dCTP + dCMP - Homo sapiens dCDP + dCDP - ? 359773 2.7.4.14 dCTP + UMP - Homo sapiens dGDP + UDP - ? 359772 2.7.4.14 dGTP + CMP - Homo sapiens dGDP + CDP - ? 359770 2.7.4.14 dGTP + CMP - Rattus norvegicus dGDP + CDP - ? 359770 2.7.4.14 dGTP + UMP - Homo sapiens dGDP + UDP - ? 359771 2.7.4.14 dTTP + CMP - Rattus norvegicus dTDP + CDP - ? 359764 2.7.4.14 dUTP + CMP - Rattus norvegicus dUDP + CDP - ? 359769 2.7.4.14 GTP + CMP - Homo sapiens GDP + CDP - ? 359775 2.7.4.14 GTP + CMP - Rattus norvegicus GDP + CDP - ? 359775 2.7.4.14 GTP + dCMP - Homo sapiens GDP + dCDP - ? 359776 2.7.4.14 GTP + UMP - Homo sapiens GDP + UDP - ? 359777 2.7.4.14 ITP + CMP - Rattus norvegicus IDP + CDP - ? 359761 2.7.4.14 ITP + UMP - Rattus norvegicus IDP + UDP - ? 359760 2.7.4.14 additional information formation of a ternary complex, addition of substrates is random Homo sapiens ? - ? 89 2.7.4.14 additional information the UMP-CMP kinase has a relaxed enantiospecificity for the nucleoside monophosphate acceptor site, but it is restricted to D-nucleotides at the donor site Homo sapiens ? - ? 89 2.7.4.14 additional information the enzyme is involved in the phosphorylation of nucleic acid precursors, and is critical in the ribo- and deoxyribonucleoside salvage pathway, as well as the anabolic phosphorylation of nucleoside analogues used in viral and anticancer therapies, e.g. cidofovir, which is activated by the enzyme, overview Homo sapiens ? - ? 89 2.7.4.14 additional information UMP-CMPK2 may have other functions in addition to the supply of substrates for mtDNA synthesis, it is highly expressed in leukemia cells, in bone marrow, and is tightly correlated with macrophage activation and inflammatory response Homo sapiens ? - ? 89 2.7.4.14 additional information substrate/product binding site determination and kinetics using MABA-CDP in a competition assay, overview Homo sapiens ? - ? 89 2.7.4.14 additional information the enzyme shows enantioselectivity, acceptor-binding site structure of human UMP-CMP kinase in the closed form or in ligand-bound forms, overview Homo sapiens ? - ? 89 2.7.4.14 additional information hyperensitivity and resistance against anti-cancer drugs in the different cervical tumour cell lines, effects of changes on UMP/CMPK1 expression on CTP and UTP synthesis , overview Homo sapiens ? - ? 89 2.7.4.14 additional information CMP and UMP are the preferred substrates for purified CMPK, and it has lower affinity to dCMP and dUMP Homo sapiens ? - ? 89 2.7.4.14 additional information mutants P64T/R134M and R134M show no activity with all substrates Homo sapiens ? - ? 89 2.7.4.14 TTP + CMP - Homo sapiens TDP + CDP - ? 359766 2.7.4.14 TTP + dCMP - Homo sapiens TDP + dCMP - ? 359767 2.7.4.14 TTP + UMP - Homo sapiens TDP + UDP - ? 359765 2.7.4.14 UTP + CMP - Rattus norvegicus UDP + CDP - ? 359768 2.7.4.14 XTP + CMP - Rattus norvegicus XDP + CDP - ? 359762