2.7.1.165 ADP + (R)-glycerate at 32% of the activity with ATP Pyrococcus horikoshii AMP + 3-phospho-(R)-glycerate - ? 387811 2.7.1.165 ADP + (R)-glycerate 32% of the activity with ATP Pyrococcus horikoshii AMP + 2-phospho-(R)-glycerate - ? 396353 2.7.1.165 ADP + (R)-glycerate 76% of the activity with GTP Sulfurisphaera tokodaii AMP + 2-phospho-(R)-glycerate - ? 396353 2.7.1.165 ADP + (R)-glycerate 76% of the activity with GTP Sulfurisphaera tokodaii 7 AMP + 2-phospho-(R)-glycerate - ? 396353 2.7.1.165 ADP + D-glycerate 76% activity compared to GTP Sulfurisphaera tokodaii AMP + 2-phospho-D-glycerate - ? 408328 2.7.1.165 AMP + (R)-glycerate 54% of the activity with GTP Sulfurisphaera tokodaii adenosine + 2-phospho-(R)-glycerate - ? 404297 2.7.1.165 AMP + D-glycerate 54% activity compared to GTP Sulfurisphaera tokodaii adenosine + 2-phospho-D-glycerate - ? 408382 2.7.1.165 ATP + (R)-glycerate - Thermoplasma acidophilum ADP + 3-phospho-(R)-glycerate - ? 357487 2.7.1.165 ATP + (R)-glycerate - Pyrococcus horikoshii ADP + 3-phospho-(R)-glycerate - ? 357487 2.7.1.165 ATP + (R)-glycerate - Thermoproteus tenax ADP + 3-phospho-(R)-glycerate - ? 357487 2.7.1.165 ATP + (R)-glycerate - Picrophilus torridus ADP + 3-phospho-(R)-glycerate - ? 357487 2.7.1.165 ATP + (R)-glycerate - Pseudomonas sp. ADP + 3-phospho-(R)-glycerate 2-phosphoglycerate ? 357487 2.7.1.165 ATP + (R)-glycerate - Hyphomicrobium sp. ADP + 3-phospho-(R)-glycerate 2-phosphoglycerate ? 357487 2.7.1.165 ATP + (R)-glycerate activity towards L-glycerate is 13% of that towards D-glycerate Hyphomicrobium methylovorum ADP + 3-phospho-(R)-glycerate 2-phosphoglycerate ir 357487 2.7.1.165 ATP + (R)-glycerate - Escherichia coli ADP + 2-phospho-(R)-glycerate - ? 394708 2.7.1.165 ATP + (R)-glycerate - Thermoproteus tenax ADP + 2-phospho-(R)-glycerate - ? 394708 2.7.1.165 ATP + (R)-glycerate - Pyrococcus horikoshii ADP + 2-phospho-(R)-glycerate - ? 394708 2.7.1.165 ATP + (R)-glycerate - Synechocystis sp. ADP + 2-phospho-(R)-glycerate - ? 394708 2.7.1.165 ATP + (R)-glycerate - Escherichia coli K-12 ADP + 2-phospho-(R)-glycerate - ? 394708 2.7.1.165 ATP + (R)-glycerate enzyme of the branched Entner-Doudoroff pathway Thermoproteus tenax ADP + 2-phospho-(R)-glycerate - ? 394708 2.7.1.165 ATP + (R)-glycerate enzymes in the (D)-glucarate/galactarate catabolic pathway Escherichia coli ADP + 2-phospho-(R)-glycerate - ? 394708 2.7.1.165 ATP + (R)-glycerate key enzyme in the Entner-Doudoroff pathway in archaea Sulfurisphaera tokodaii ADP + 2-phospho-(R)-glycerate - ? 394708 2.7.1.165 ATP + (R)-glycerate 92% of the activity with GTP Sulfurisphaera tokodaii ADP + 2-phospho-(R)-glycerate - ? 394708 2.7.1.165 ATP + (R)-glycerate no activity with (S)-glycerate Escherichia coli ADP + 2-phospho-(R)-glycerate - ? 394708 2.7.1.165 ATP + (R)-glycerate key enzyme in the Entner-Doudoroff pathway in archaea Sulfurisphaera tokodaii 7 ADP + 2-phospho-(R)-glycerate - ? 394708 2.7.1.165 ATP + (R)-glycerate 92% of the activity with GTP Sulfurisphaera tokodaii 7 ADP + 2-phospho-(R)-glycerate - ? 394708 2.7.1.165 ATP + D-glycerate - Escherichia coli ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate - Homo sapiens ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate - Zea mays ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate - Arabidopsis thaliana ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate - Saccharolobus solfataricus ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate - Pyrococcus horikoshii ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate specific substrate Saccharolobus solfataricus ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate 92% activity compared to GTP Sulfurisphaera tokodaii ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate a key enzyme in the nonphosphorylative Entner-Doudoroff pathway in archaea. The glycerate kinase, with its unusual regulatory properties, seems to play a major role in controlling the flux between the glycolytic nonphosphorylative Entner-Doudoroff and the glycolytic/gluconeogenetic semiphosphorylative Entner-Doudoroff pathway Saccharolobus solfataricus ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate the enzyme is specific for D-glycerate. The enzyme shows cooperativity by D-glycerate and ATP. A good fit achieved from the kinetic model to the experimental data suggests a mechanism where two glycerate molecules bind to glycerate kinase and are converted to product, while a third binding site appears to be inhibitory. The model suggests that the inhibition appears to be due to the formation of an additional complex with very low activity at 80°C Saccharolobus solfataricus ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate no activity with DL-glyceraldehyde, 2-phospho-D-glycerate, 3-phospho-D-glycerate or L-tartrate. Activity with D-gluconate or glycerol at 5% of the activity compared to D-glycerate. Specificity for ATP as a phosphate donor. GTP, CTP, and UTP exhibit 0~4% of the activity of ATP Thermoplasma acidophilum ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate - Saccharolobus solfataricus P2 ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate specific substrate Saccharolobus solfataricus P2 ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate a key enzyme in the nonphosphorylative Entner-Doudoroff pathway in archaea. The glycerate kinase, with its unusual regulatory properties, seems to play a major role in controlling the flux between the glycolytic nonphosphorylative Entner-Doudoroff and the glycolytic/gluconeogenetic semiphosphorylative Entner-Doudoroff pathway Saccharolobus solfataricus P2 ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate the enzyme is specific for D-glycerate. The enzyme shows cooperativity by D-glycerate and ATP. A good fit achieved from the kinetic model to the experimental data suggests a mechanism where two glycerate molecules bind to glycerate kinase and are converted to product, while a third binding site appears to be inhibitory. The model suggests that the inhibition appears to be due to the formation of an additional complex with very low activity at 80°C Saccharolobus solfataricus P2 ADP + 2-phospho-D-glycerate - ? 408498 2.7.1.165 ATP + D-glycerate - Thermotoga maritima ADP + 2-phospho-(R)-glycerate - ? 457117 2.7.1.165 ATP + D-glycerate - Escherichia coli ADP + 2-phospho-(R)-glycerate - ? 457117 2.7.1.165 ATP + D-glycerate the enzyme shows selectivity and excellent enantioselectivity towards phosphorylation of the D-enantiomer of glyceric acid Thermotoga maritima ADP + 2-phospho-(R)-glycerate - ? 457117 2.7.1.165 ATP + D-glycerate - Thermotoga maritima DSM 3109 ADP + 2-phospho-(R)-glycerate - ? 457117 2.7.1.165 ATP + D-glycerate the enzyme shows selectivity and excellent enantioselectivity towards phosphorylation of the D-enantiomer of glyceric acid Thermotoga maritima DSM 3109 ADP + 2-phospho-(R)-glycerate - ? 457117 2.7.1.165 ATP + D-glycerate - Thermotoga maritima ATCC 43589 ADP + 2-phospho-(R)-glycerate - ? 457117 2.7.1.165 ATP + D-glycerate the enzyme shows selectivity and excellent enantioselectivity towards phosphorylation of the D-enantiomer of glyceric acid Thermotoga maritima ATCC 43589 ADP + 2-phospho-(R)-glycerate - ? 457117 2.7.1.165 ATP + D-glycerate - Thermotoga maritima JCM 10099 ADP + 2-phospho-(R)-glycerate - ? 457117 2.7.1.165 ATP + D-glycerate the enzyme shows selectivity and excellent enantioselectivity towards phosphorylation of the D-enantiomer of glyceric acid Thermotoga maritima JCM 10099 ADP + 2-phospho-(R)-glycerate - ? 457117 2.7.1.165 CTP + (R)-glycerate at 59% of the activity with ATP Hyphomicrobium methylovorum CDP + 3-phospho-(R)-glycerate - ? 357489 2.7.1.165 CTP + (R)-glycerate at 73% of the activity with ATP Pyrococcus horikoshii CDP + 3-phospho-(R)-glycerate - ? 357489 2.7.1.165 CTP + (R)-glycerate 16% of the activity with ATP Thermoproteus tenax CDP + 2-phospho-(R)-glycerate - ? 396793 2.7.1.165 CTP + (R)-glycerate 73% of the activity with ATP Pyrococcus horikoshii CDP + 2-phospho-(R)-glycerate - ? 396793 2.7.1.165 CTP + (R)-glycerate 87% of the activity with GTP Sulfurisphaera tokodaii CDP + 2-phospho-(R)-glycerate - ? 396793 2.7.1.165 CTP + (R)-glycerate 87% of the activity with GTP Sulfurisphaera tokodaii 7 CDP + 2-phospho-(R)-glycerate - ? 396793 2.7.1.165 CTP + D-glycerate 87% activity compared to GTP Sulfurisphaera tokodaii CDP + 2-phospho-D-glycerate - ? 408928 2.7.1.165 diphosphate + (R)-glycerate at 112% of the activity with ATP Pyrococcus horikoshii phosphate + 3-phospho-(R)-glycerate - ? 388588 2.7.1.165 diphosphate + (R)-glycerate 112% of the activity with ATP Pyrococcus horikoshii phosphate + 2-phospho-(R)-glycerate - ? 397075 2.7.1.165 diphosphate + (R)-glycerate 63% of the activity with ATP Sulfurisphaera tokodaii phosphate + 2-phospho-(R)-glycerate - ? 397075 2.7.1.165 diphosphate + (R)-glycerate 63% of the activity with ATP Sulfurisphaera tokodaii 7 phosphate + 2-phospho-(R)-glycerate - ? 397075 2.7.1.165 diphosphate + D-glycerate 63% activity compared to GTP Sulfurisphaera tokodaii phosphate + 2-phospho-D-glycerate - ? 409321 2.7.1.165 GTP + (R)-glycerate at 59% of the activity with ATP Hyphomicrobium methylovorum GDP + 3-phospho-(R)-glycerate - ? 357488 2.7.1.165 GTP + (R)-glycerate at 64% of the activity with ATP Pyrococcus horikoshii GDP + 3-phospho-(R)-glycerate - ? 357488 2.7.1.165 GTP + (R)-glycerate - Sulfurisphaera tokodaii GDP + 2-phospho-(R)-glycerate - ? 397365 2.7.1.165 GTP + (R)-glycerate 20% of the activity with ATP Thermoproteus tenax GDP + 2-phospho-(R)-glycerate - ? 397365 2.7.1.165 GTP + (R)-glycerate 64% of the activity with ATP Pyrococcus horikoshii GDP + 2-phospho-(R)-glycerate - ? 397365 2.7.1.165 GTP + D-glycerate 100% activity towards GTP Sulfurisphaera tokodaii GDP + 2-phospho-D-glycerate - ? 409561 2.7.1.165 additional information AMP is not a substrate Pyrococcus horikoshii ? - ? 89 2.7.1.165 additional information ATP can be partially replaced by GTP, CTP, TTP and UTP (16%, 20%, 16%, and 10% activity, respectively) Thermoproteus tenax ? - ? 89 2.7.1.165 additional information GTP, CTP, UTP, D-glucose, D-gluconate, glycerol, D-fructose, DL-glyceraldehyde, D-ribose, D-xylose, 2-phospho-D-glycerate, 3-phospho-D-glycerate, and L-tartrate are no substrates Thermoplasma acidophilum ? - ? 89 2.7.1.165 additional information L-glycerate, galactonate, gluconate, malate, pyruvate, lactate, glyceraldehyde, glycerol, serine, 3-phosphoglycerate, ADP, diphosphate, polyphosphate, and glyceraldehyde 3-phosphate are no substrates Thermoproteus tenax ? - ? 89 2.7.1.165 additional information the enzyme shows highest activity with D-glycerate (100%), and lower activity (34%) with L-glycerate Picrophilus torridus ? - ? 89 2.7.1.165 additional information no activity is observed with ADP, diphosphate and polyphosphates Thermoproteus tenax ? - ? 89 2.7.1.165 additional information biocatalytic phosphorylations catalyzed by recombinant glycerate-2-kinase are detected with quantitative 31P NMR spectroscopy using a phosphoenolpyruvate (PEP)/pyruvate kinase system for ATP regeneration, starting with racemic and the enantiopure D- and L-glycerate as substrate. Nearly 100% conversion of D-glycerate to D-glycerate-2-phosphate is observed. No activity with pure L-glycerate as substrate, DL-glycerate gives a 50% conversion with excellent enantioselectivity. Optimization of the reaction system for the biocatalytic phosphorylation of D-glyceric acid, upscaling, overview Thermotoga maritima ? - - 89 2.7.1.165 additional information biocatalytic phosphorylations catalyzed by recombinant glycerate-2-kinase are detected with quantitative 31P NMR spectroscopy using a phosphoenolpyruvate (PEP)/pyruvate kinase system for ATP regeneration, starting with racemic and the enantiopure D- and L-glycerate as substrate. Nearly 100% conversion of D-glycerate to D-glycerate-2-phosphate is observed. No activity with pure L-glycerate as substrate, DL-glycerate gives a 50% conversion with excellent enantioselectivity. Optimization of the reaction system for the biocatalytic phosphorylation of D-glyceric acid, upscaling, overview Thermotoga maritima DSM 3109 ? - - 89 2.7.1.165 additional information biocatalytic phosphorylations catalyzed by recombinant glycerate-2-kinase are detected with quantitative 31P NMR spectroscopy using a phosphoenolpyruvate (PEP)/pyruvate kinase system for ATP regeneration, starting with racemic and the enantiopure D- and L-glycerate as substrate. Nearly 100% conversion of D-glycerate to D-glycerate-2-phosphate is observed. No activity with pure L-glycerate as substrate, DL-glycerate gives a 50% conversion with excellent enantioselectivity. Optimization of the reaction system for the biocatalytic phosphorylation of D-glyceric acid, upscaling, overview Thermotoga maritima ATCC 43589 ? - - 89 2.7.1.165 additional information biocatalytic phosphorylations catalyzed by recombinant glycerate-2-kinase are detected with quantitative 31P NMR spectroscopy using a phosphoenolpyruvate (PEP)/pyruvate kinase system for ATP regeneration, starting with racemic and the enantiopure D- and L-glycerate as substrate. Nearly 100% conversion of D-glycerate to D-glycerate-2-phosphate is observed. No activity with pure L-glycerate as substrate, DL-glycerate gives a 50% conversion with excellent enantioselectivity. Optimization of the reaction system for the biocatalytic phosphorylation of D-glyceric acid, upscaling, overview Thermotoga maritima JCM 10099 ? - - 89 2.7.1.165 TTP + (R)-glycerate 16% of the activity with ATP Thermoproteus tenax TDP + 2-phospho-(R)-glycerate - ? 398465 2.7.1.165 UTP + (R)-glycerate at 64% of the activity with ATP Hyphomicrobium methylovorum UDP + 3-phospho-(R)-glycerate - ? 357490 2.7.1.165 UTP + (R)-glycerate at 29% of the activity with ATP Pyrococcus horikoshii UDP + 3-phospho-(R)-glycerate - ? 357490 2.7.1.165 UTP + (R)-glycerate 10% of the activity with ATP Thermoproteus tenax UDP + 2-phospho-(R)-glycerate - ? 398557 2.7.1.165 UTP + (R)-glycerate 29% of the activity with ATP Pyrococcus horikoshii UDP + 2-phospho-(R)-glycerate - ? 398557 2.7.1.165 UTP + (R)-glycerate 81% of the activity with GTP Sulfurisphaera tokodaii UDP + 2-phospho-(R)-glycerate - ? 398557 2.7.1.165 UTP + D-glycerate 81% activity compared to GTP Sulfurisphaera tokodaii UDP + 2-phospho-D-glycerate - ? 410628