2.7.1.1 2'-dATP + D-glucose 73% of the activity with ATP Drosophila melanogaster 2'-dADP + D-glucose 6-phosphate - ? 356929 2.7.1.1 2'-dATP + D-glucose 73-80% of the activity with ATP, isoenzyme Hex A, B, C Ascaris suum 2'-dADP + D-glucose 6-phosphate - ? 356929 2.7.1.1 2'-dATP + D-glucose Hex A: 73% of the activity with ATP, Hex B: 76% of the activity with ATP, Hex C: 80% of the activity with ATP Drosophila melanogaster 2'-dADP + D-glucose 6-phosphate - ? 356929 2.7.1.1 2-fluoro-2-deoxy-D-glucose + ATP - Rattus norvegicus 2-fluoro-2-deoxy-D-glucose 6-phosphate + ADP - ? 356927 2.7.1.1 2-fluoro-2-deoxy-D-glucose + ATP - Bos taurus 2-fluoro-2-deoxy-D-glucose 6-phosphate + ADP - ? 356927 2.7.1.1 ATP + 1,5-anhydro-D-glucitol weak Saccharomyces cerevisiae ADP + 1,5-anhydro-D-glucitol 6-phosphate - ? 356933 2.7.1.1 ATP + 1-thio-D-glucose isoenzyme II, weak Saccharomyces cerevisiae ADP + 1-thio-D-glucose 6-phosphate - ? 356934 2.7.1.1 ATP + 2-deoxy-2-fluoro-D-glucose - Bos taurus ADP + 2-deoxy-2-fluoro-D-glucose 6-phosphate - ? 356935 2.7.1.1 ATP + 2-deoxy-2-fluoro-D-glucose good substrate Saccharomyces cerevisiae ADP + 2-deoxy-2-fluoro-D-glucose 6-phosphate - ? 356935 2.7.1.1 ATP + 2-deoxy-2-fluoro-D-glucose good substrate Bos taurus ADP + 2-deoxy-2-fluoro-D-glucose 6-phosphate - ? 356935 2.7.1.1 ATP + 2-deoxy-D-glucose - Homo sapiens ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose - Rattus norvegicus ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose - Sus scrofa ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose - Bos taurus ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose - Aspergillus niger ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose - Ascaris suum ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose - Ogataea angusta ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose - Lithobates catesbeianus ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose - Aeropyrum pernix ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose - Thermoproteus tenax ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose - Trypanosoma brucei ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose - Sulfurisphaera tokodaii ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose - Eimeria tenella ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose 30% of the activity with glucose Bubalus bubalis ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose Hex Ia: 95% of the activity with glucose, Hex Ib: 99% of the activity with glucose, Hex Ic: 74% of the activity with glucose Homo sapiens ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose hexokinase Ia: 65% of the activity with glucose, hexokinase Ib: 70% of the activity with glucose Oryctolagus cuniculus ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose hexokinase III: 151% of the activity with glucose Sus scrofa ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose 54% of the activity with glucose Oryctolagus cuniculus ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose Hex A: 93% of the activity with glucose, Hex B: 51% of the activity with glucose, Hex C: 48% of the activity with glucose Drosophila melanogaster ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose 93% of the activity with glucose Drosophila melanogaster ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose 65.7% of the activity with glucose, erythrocyte enzyme Oryctolagus cuniculus ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose hexokinase I: as effective as glucose Homo sapiens ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose 84% of the activity with glucose Oryctolagus cuniculus ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose 17% of activity with D-fructose at 50 mM substrate concentration Ogataea angusta ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose 42% of the activity with D-glucose Thermus caldophilus ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose sugar binding to recombinant wild-type and mutant glucokinase monitored by kinetic measurement and tryptophan fluorescence Homo sapiens ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose - Sulfurisphaera tokodaii 7 ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose 42% of the activity with D-glucose Thermus caldophilus GK24 ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 2-deoxy-D-glucose Hex A: 93% of the activity with glucose, Hex B: 51% of the activity with glucose, Hex C: 48% of the activity with glucose Drosophila melanogaster Samarkand ADP + 2-deoxy-D-glucose 6-phosphate - ? 356924 2.7.1.1 ATP + 3-deoxy-3-amino-D-glucose isoenzyme II, weak Saccharomyces cerevisiae ADP + 3-deoxy-3-amino-D-glucose 6-phosphate - ? 356937 2.7.1.1 ATP + 5-thio-D-glucose very slow phosphorylation Saccharomyces cerevisiae ADP + 5-thio-D-glucose 6-phosphate - ? 356938 2.7.1.1 ATP + 5-thio-D-glucose very slow phosphorylation Bos taurus ADP + 5-thio-D-glucose 6-phosphate - ? 356938 2.7.1.1 ATP + beta-D-glucose - Homo sapiens ADP + beta-D-glucose 6-phosphate - ? 422887 2.7.1.1 ATP + D-allose 58% of the activity with D-glucose Thermus caldophilus ADP + D-allose 6-phosphate - ? 353957 2.7.1.1 ATP + D-allose phosphorylation of D-allose at C6 by hexokinase is essential since it up-regulates expression of transcription factor OsABF1 Oryza sativa ADP + D-allose 6-phosphate - ? 353957 2.7.1.1 ATP + D-allose 58% of the activity with D-glucose Thermus caldophilus GK24 ADP + D-allose 6-phosphate - ? 353957 2.7.1.1 ATP + D-fructose - Homo sapiens ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose - Saccharomyces cerevisiae ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose - Nicotiana tabacum ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose - Eimeria tenella ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose - Aeropyrum pernix ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose - Botrytis cinerea ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose - Brugia malayi ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose - Solanum chacoense ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose - Helianthus annuus ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose - Thermus thermophilus ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose - Sulfurisphaera tokodaii ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose - Macrobrachium nipponense ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose - Clonorchis sinensis ADP + D-fructose 6-phosphate 16.6% of the activity with ATP ? 357326 2.7.1.1 ATP + D-fructose - Cryptosporidium parvum ADP + D-fructose 6-phosphate 8.6% of the activity with ATP ? 357326 2.7.1.1 ATP + D-fructose best substrate Ogataea angusta ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose 16% of the activity with D-glucose Thermus caldophilus ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose isozyme HKI, isozyme HKII is highly specific for D-fructose Brassica rapa subsp. oleifera ADP + D-fructose 6-phosphate - ? 357326 2.7.1.1 ATP + D-fructose - Cryptosporidium parvum Iowa II ADP + D-fructose 6-phosphate 8.6% of the activity with ATP ? 357326 2.7.1.1 ATP + D-fructose - Lysinibacillus sphaericus ? - ? 388008 2.7.1.1 ATP + D-galactose - Thermus thermophilus ADP + D-galactose 6-phosphate - ? 353960 2.7.1.1 ATP + D-galactose - Brugia malayi ? - ? 396473 2.7.1.1 ATP + D-glucosamine - Aeropyrum pernix ADP + D-glucosamine 6-phosphate - ? 357325 2.7.1.1 ATP + D-glucosamine - Sulfurisphaera tokodaii ADP + D-glucosamine 6-phosphate - ? 357325 2.7.1.1 ATP + D-glucosamine 67% of the activity with D-glucose Thermus caldophilus ADP + D-glucosamine 6-phosphate - ? 357325 2.7.1.1 ATP + D-glucosamine 8% of activity with D-fructose at 50 mM substrate concentration Ogataea angusta ADP + D-glucosamine 6-phosphate - ? 357325 2.7.1.1 ATP + D-glucosamine 67% of the activity with D-glucose Thermus caldophilus GK24 ADP + D-glucosamine 6-phosphate - ? 357325 2.7.1.1 ATP + D-glucose - Gallus gallus ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Mammalia ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Mus musculus ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Thermus thermophilus ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Homo sapiens ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Rattus norvegicus ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Saccharomyces cerevisiae ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Triticum aestivum ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Pisum sativum ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Zea mays ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Solanum tuberosum ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Nicotiana tabacum ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Trypanosoma brucei ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Canis lupus familiaris ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Xenopus laevis ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Ogataea angusta ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Trypanosoma cruzi ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Felis catus ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Carassius auratus ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Eimeria tenella ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Cyprinus carpio ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Oncorhynchus mykiss ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Littorina littorea ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Aeropyrum pernix ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Kluyveromyces lactis ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Bos taurus ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Clonorchis sinensis ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Lysinibacillus sphaericus ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Botrytis cinerea ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Capsicum chacoense ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Brugia malayi ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Lithobates sylvaticus ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Urocitellus undulatus ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Solanum chacoense ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Helianthus annuus ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Sulfurisphaera tokodaii ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Plasmodium falciparum ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Macrobrachium nipponense ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose best substrate Thermus caldophilus ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose enzyme mediates glucose repression Ogataea angusta ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose enzyme plays a key role in whole-body glucose homeostasis Homo sapiens ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose first step in glucose metabolism, pathway overview, isozyme types are differently regulated and catalytically active, isozyme type I is involved in catabolism, while type II and III are involved in anabolism, the activity rate of type I isozyme i correlated with the oxidative phosphorylation rate, overview Mammalia ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose first step in the process of glycolysis Saccharomyces cerevisiae ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose involved in glucose catabolism Bos taurus ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose isozyme hexokinase 2 plays a role in starch and secondary metabolism in starch sheath, xylem parenchyma, guard cells, and root tips Nicotiana tabacum ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose the pancreatic enzyme is imporatnt as glucose sensor in insulin secretion and blood glucose homeostasis, interaction of ATP and glucose during binding to the enzyme Homo sapiens ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose 39% of activity with D-fructose at 50 mM substrate concentration Ogataea angusta ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose isozyme HKI Brassica rapa subsp. oleifera ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose control of GK activity and Km through the ratio of distinct conformers (super-open, open, and closed) through either substrate or other ligand binding and/or dissociation Homo sapiens ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose the presence of 0.5 mM glucose induced total hexokinase activity in supernatants from sperm extracts of 1.7 mIU/mg protein, while the same concentration of both fructose, mannose, and sorbitol induced total hexokinase activity from 0.3 mIU/mg protein to 0.60 IU/mg protein. Diluted boar sperm from fresh ejaculates phosphorylates glucose through the hexokinase step much more efficiently than fructose or mannose. This difference facilitates a much more rapid intake of glucose into glycolysis than the other sugars Sus scrofa ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose sugar binding to recombinant wild-type and mutant glucokinase monitored by kinetic measurement and tryptophan fluorescence Homo sapiens ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose hexokinase II displays high affinity for D-glucose Homo sapiens ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose under physiological conditions D-glucose is the preferred substrate Homo sapiens ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose in Sulfolobus tokodaii this is the sole enzyme responsible for the phosphorylation of glucose in vivo Sulfurisphaera tokodaii ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose ATP is the preferred nucleside triphosphate Cryptosporidium parvum ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose the enzyme shows a narrow substrate specificity, as it does not display activity towards fructose, mannose or galactose Trypanosoma brucei ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Sulfurisphaera tokodaii 7 ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose in Sulfolobus tokodaii this is the sole enzyme responsible for the phosphorylation of glucose in vivo Sulfurisphaera tokodaii 7 ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose - Thermus thermophilus DSM 579 ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose best substrate Thermus caldophilus GK24 ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-glucose ATP is the preferred nucleside triphosphate Cryptosporidium parvum Iowa II ADP + D-glucose 6-phosphate - ? 353958 2.7.1.1 ATP + D-hexose - Rattus norvegicus ADP + D-hexose 6-phosphate - ? 452233 2.7.1.1 ATP + D-maltose - Brugia malayi ? - ? 396475 2.7.1.1 ATP + D-mannosamine fairly good substrate Saccharomyces cerevisiae ADP + D-mannosamine 6-phosphate - ? 356936 2.7.1.1 ATP + D-mannosamine fairly good substrate Bos taurus ADP + D-mannosamine 6-phosphate - ? 356936 2.7.1.1 ATP + D-mannose - Thermus thermophilus ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose - Homo sapiens ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose - Saccharomyces cerevisiae ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose - Ogataea angusta ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose - Aeropyrum pernix ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose - Nicotiana tabacum ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose - Brugia malayi ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose - Solanum chacoense ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose - Helianthus annuus ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose - Sulfurisphaera tokodaii ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose - Cryptosporidium parvum ADP + D-mannose 6-phosphate 52% of the activity with ATP ? 357327 2.7.1.1 ATP + D-mannose - Clonorchis sinensis ADP + D-mannose 6-phosphate 97% of the activity with ATP ? 357327 2.7.1.1 ATP + D-mannose poor substrate Eimeria tenella ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose 33% of activity with D-fructose at 50 mM substrate concentration Ogataea angusta ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose 75% of the activity with D-glucose Thermus caldophilus ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose sugar binding to recombinant wild-type and mutant glucokinase monitored by kinetic measurement and tryptophan fluorescence Homo sapiens ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose - Thermus thermophilus DSM 579 ADP + D-mannose 6-phosphate - ? 357327 2.7.1.1 ATP + D-mannose - Cryptosporidium parvum Iowa II ADP + D-mannose 6-phosphate 52% of the activity with ATP ? 357327 2.7.1.1 ATP + D-mannose - Lysinibacillus sphaericus ? - ? 357666 2.7.1.1 ATP + hexose - Canis lupus familiaris ADP + hexose 6-phosphate - ? 378520 2.7.1.1 ATP + mannoheptulose sugar binding to recombinant wild-type and mutant glucokinase monitored by kinetic measurement and tryptophan fluorescence Homo sapiens ? - ? 357451 2.7.1.1 ATP + N-acetyl-alpha-D-glucosamine sugar binding to recombinant wild-type and mutant glucokinase monitored by kinetic measurement and tryptophan fluorescence Homo sapiens ADP + N-acetyl-alpha-D-glucosamine 6-phosphate - ? 357324 2.7.1.1 ATP + N-acetyl-D-glucosamine - Sulfurisphaera tokodaii ADP + N-acetyl-D-glucosamine 6-phosphate - ? 357661 2.7.1.1 CTP + D-glucose - Homo sapiens CDP + D-glucose 6-phosphate - ? 356930 2.7.1.1 CTP + D-glucose - Sus scrofa CDP + D-glucose 6-phosphate - ? 356930 2.7.1.1 CTP + D-glucose - Oryctolagus cuniculus CDP + D-glucose 6-phosphate - ? 356930 2.7.1.1 CTP + D-glucose - Solanum chacoense CDP + D-glucose 6-phosphate - ? 356930 2.7.1.1 CTP + D-glucose - Helianthus annuus CDP + D-glucose 6-phosphate - ? 356930 2.7.1.1 CTP + D-glucose - Cryptosporidium parvum CDP + D-glucose 6-phosphate - ? 356930 2.7.1.1 CTP + D-glucose - Clonorchis sinensis CDP + D-glucose 6-phosphate 18% of the activity with ATP ? 356930 2.7.1.1 CTP + D-glucose MgCTP, hexokinase I: 5% of the activity with ATP Homo sapiens CDP + D-glucose 6-phosphate - ? 356930 2.7.1.1 CTP + D-glucose 11% of the activity with ATP, isoenzyme Hex C, not: isoenzyme Hex A and Hex B Ascaris suum CDP + D-glucose 6-phosphate - ? 356930 2.7.1.1 CTP + D-glucose 35% of the activity with ATP Saccharomyces cerevisiae CDP + D-glucose 6-phosphate - ? 356930 2.7.1.1 CTP + D-glucose 35% of the activity with ATP Catharanthus roseus CDP + D-glucose 6-phosphate - ? 356930 2.7.1.1 CTP + D-glucose Hex B: 5% of the activity with ATP, Hex C: 11% of the activity with ATP, not Hex A Drosophila melanogaster CDP + D-glucose 6-phosphate - ? 356930 2.7.1.1 CTP + D-glucose 81% of the activity with ATP Sulfurisphaera tokodaii CDP + D-glucose 6-phosphate - ? 356930 2.7.1.1 CTP + D-glucose 35% of the activity with ATP Catharanthus roseus B/TH CDP + D-glucose 6-phosphate - ? 356930 2.7.1.1 CTP + D-glucose - Cryptosporidium parvum Iowa II CDP + D-glucose 6-phosphate - ? 356930 2.7.1.1 D-fructose + ATP - Rattus norvegicus ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP - Saccharomyces cerevisiae ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP - Aspergillus niger ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP - Ascaris suum ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP - Lithobates catesbeianus ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP - Trypanosoma cruzi ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP - Catharanthus roseus ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP - Thermoproteus tenax ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP - Trypanosoma brucei ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP poor substrate Rattus norvegicus ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP hexokinase C Rattus norvegicus ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP 46.3% of the activity with glucose Bubalus bubalis ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP HK3: 16% of the activity with glucose Solanum tuberosum ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP hexokinase III: 12.6% of the activity with glucose Sus scrofa ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP hexokinase I: 130% of the activity with glucose Homo sapiens ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP 70% of the activity with glucose, erythrocyte enzyme Oryctolagus cuniculus ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP 79% of the activity with glucose Toxoplasma gondii ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP hexokinase Ia: 69% of the activity with glucose, hexokinase Ib: 42% of the activity with glucose Oryctolagus cuniculus ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP hexokinase, but not glucokinase Schwanniomyces occidentalis ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP HK1: 4% of the activity with glucose, HK2: 3% of the activity with glucose Solanum tuberosum ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP higher activity than with glucose or mannose Schizosaccharomyces pombe ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP Hex Ia: 100% of the activity with glucose, Hex Ib: 120% of the activity with glucose, Hex Ic: 90% of the activity with glucose Homo sapiens ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP hexokinases PI and PII phosphorylate fructose 2.5 and 1.5times faster than glucose, glucokinase: very low or no activity Saccharomyces cerevisiae ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP 110% of the activity with glucose Oryctolagus cuniculus ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP Hex A: 50% of the activity with glucose, Hex B: 41% of the activity with glucose, Hex C: 134% of the activity with glucose Drosophila melanogaster ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP 50% of the activity with glucose Drosophila melanogaster ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP HK3: 5% of the activity with glucose Solanum tuberosum ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP - Catharanthus roseus B/TH ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP higher activity than with glucose or mannose Schizosaccharomyces pombe PB003 ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP hexokinases PI and PII phosphorylate fructose 2.5 and 1.5times faster than glucose, glucokinase: very low or no activity Saccharomyces cerevisiae G-517 / CECT 1317 ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP Hex A: 50% of the activity with glucose, Hex B: 41% of the activity with glucose, Hex C: 134% of the activity with glucose Drosophila melanogaster Samarkand ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP 50% of the activity with glucose Drosophila melanogaster Samarkand ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP higher activity than with glucose or mannose Schizosaccharomyces pombe PB004 ADP + D-fructose 6-phosphate - ? 356923 2.7.1.1 D-fructose + ATP - Leishmania mexicana D-fructose 6-phosphate + ADP - ? 370507 2.7.1.1 D-fructose + ATP fructose metabolism Catharanthus roseus D-fructose 6-phosphate + ADP - ? 370507 2.7.1.1 D-glucosamine + ATP - Homo sapiens ADP + D-glucosamine 6-phosphate - ? 356925 2.7.1.1 D-glucosamine + ATP - Rattus norvegicus ADP + D-glucosamine 6-phosphate - ? 356925 2.7.1.1 D-glucosamine + ATP - Oryctolagus cuniculus ADP + D-glucosamine 6-phosphate - ? 356925 2.7.1.1 D-glucosamine + ATP - Trypanosoma brucei ADP + D-glucosamine 6-phosphate - ? 356925 2.7.1.1 D-glucosamine + ATP Hex A: 19% of the activity with glucose, Hex C: 44% of the activity with glucose, not Hex B Drosophila melanogaster ADP + D-glucosamine 6-phosphate - ? 356925 2.7.1.1 D-glucosamine + ATP Hex Ia and Ib: 47% of the activity with glucose, Hex Ic: 21% of the activity with glucose Homo sapiens ADP + D-glucosamine 6-phosphate - ? 356925 2.7.1.1 D-glucosamine + ATP hexokinase III: 35% of the activity with glucose Sus scrofa ADP + D-glucosamine 6-phosphate - ? 356925 2.7.1.1 D-glucosamine + ATP 44.9% of the activity with glucose Bubalus bubalis ADP + D-glucosamine 6-phosphate - ? 356925 2.7.1.1 D-glucosamine + ATP hexokinase Ia: 35% of the activity with glucose, hexokinase Ib: 70% of the activity with glucose Oryctolagus cuniculus ADP + D-glucosamine 6-phosphate - ? 356925 2.7.1.1 D-glucosamine + ATP erythrocyte enzyme, 43.4% of the activity with glucose Oryctolagus cuniculus ADP + D-glucosamine 6-phosphate - ? 356925 2.7.1.1 D-glucosamine + ATP hexokinase I: 40% of the activity with glucose Homo sapiens ADP + D-glucosamine 6-phosphate - ? 356925 2.7.1.1 D-glucosamine + ATP Hex A: 19% of the activity with glucose, Hex C: 44% of the activity with glucose, not Hex B Drosophila melanogaster Samarkand ADP + D-glucosamine 6-phosphate - ? 356925 2.7.1.1 D-glucose + ATP - Drosophila melanogaster ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Mus musculus ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Homo sapiens ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Rattus norvegicus ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Sus scrofa ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Saccharomyces cerevisiae ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Bos taurus ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Oryctolagus cuniculus ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Aspergillus niger ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Solanum tuberosum ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Trypanosoma brucei ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Ascaris suum ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Schizosaccharomyces pombe ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Lithobates catesbeianus ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Trypanosoma cruzi ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Schistosoma mansoni ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Leishmania mexicana ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Catharanthus roseus ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Rhinella marina ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Trypanosoma congolense ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP specific for ATP Candida maltosa ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP specific for ATP Toxoplasma gondii ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP good substrate Rattus norvegicus ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP good substrate Entamoeba histolytica ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP 11% of the activity with fructose Schizosaccharomyces pombe ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP specific for glucose Trypanosoma cruzi ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP bound glucose is present as the beta-anomer, glucose binding sites Homo sapiens ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP 59% of the activity with fructose Schizosaccharomyces pombe ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP preferred specificity for glucose Thermoproteus tenax ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP preferred specificity for glucose Toxoplasma gondii ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP hexokinase and glucokinase Schwanniomyces occidentalis ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP absolute requirement for D-glucose Mus musculus ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP the binding of glucose does not depend on ATP, and vice versa Drosophila melanogaster ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP most active with D-glucose Trypanosoma cruzi ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP MgATP2- Homo sapiens ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP MgATP2- Oryctolagus cuniculus ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP MgATP2- Bubalus bubalis ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP hexokinase bound at type A and B sites of brain mitochondria selectively uses intramitochondrial ATP as substrate, but hexokinase bound at type B sites, after removal of enzyme of type A sites, shows no such selectivity Bos taurus ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Catharanthus roseus B/TH ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Schizosaccharomyces pombe NCYC 132S2-2 ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP 11% of the activity with fructose Schizosaccharomyces pombe PB003 ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Saccharomyces cerevisiae G-517 / CECT 1317 ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP the binding of glucose does not depend on ATP, and vice versa Drosophila melanogaster Samarkand ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP - Drosophila melanogaster Samarkand ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP 59% of the activity with fructose Schizosaccharomyces pombe PB004 ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP good substrate Entamoeba histolytica SFL-3 ADP + D-glucose 6-phosphate - ? 356921 2.7.1.1 D-glucose + ATP glycolytic enzyme Homo sapiens D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP glycolytic enzyme Rattus norvegicus D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP glycolytic enzyme Trypanosoma congolense D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP glycolytic enzyme Trypanosoma cruzi D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP key enzyme in the control of brain glucose metabolism Mus musculus D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP cerebral glycolytic metabolism, mechanisms for regulation of mitochondrial hexokinase activity may depend on the ratio of type A:type B sites Bos taurus D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP hexokinase I governs the rate-limiting step of glycolysis in brain Homo sapiens D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP glucose metabolism Homo sapiens D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP glucose metabolism Rattus norvegicus D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP glucose metabolism Bos taurus D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP glucose metabolism Oryctolagus cuniculus D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP glucose metabolism Solanum tuberosum D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP glucose metabolism Catharanthus roseus D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP binding of brain enzyme to the outer mitochondrial membrane plays a role in regulation of hexokinase activity in vivo, and thereby of the cerebral glycolytic rate Rattus norvegicus D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP first step of the glycolytic pathway, physiological function of membrane-bound hexokinase in the tachyzoite: glucose channeling with a glucose/hexose transporter Toxoplasma gondii D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP enzyme of the glucose-phosphate branch of the oxidative pentose phosphate pathway Schizosaccharomyces pombe D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP hexokinase isoenzymes are particularly important in glycolytic flux and, in addition, hexokinase PII, hexokinase PIIM or both are also responsible for the overall regulation of carbohydrate metabolism, role in glucose repression Saccharomyces cerevisiae D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP hexokinase I is the pacemaker of glycolysis in brain tissue Homo sapiens D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP hexokinase I is a regulated enzyme and serves a critical role in controlling the rate of glycolysis in both brain and red blood cells Homo sapiens D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP organ- and development-specific changes in the abundance of the various enzyme forms contribute to the regulation of hexose metabolism Solanum tuberosum D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP rate-limiting step in the glycolysis Trypanosoma brucei D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP rate-limiting step in the glycolysis Toxoplasma gondii D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP the presence of type III hexokinase enables the adult pig erythrocytes to metabolize low but appreciable amounts of glucose Sus scrofa D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP carbohydrate metabolism Thermoproteus tenax D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP initial catalytic step of the cerebral glucose metabolism, glucose 6-phosphate represents a major regulatory influence on the in vivo activity Rattus norvegicus D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP initial enzyme of glycolysis Homo sapiens D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP initial enzyme of glycolysis Entamoeba histolytica D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP at high energy charge HXK1 may be involved in glycolysis and in some biosynthetic processes, e.g. phosphorylating mannose or glucose for the later integration into membrane components, at low energy charge HXK1 could be inhibited by AMP and ADP, and HXK2 would be more active and could keep up glucokinase activity for glycolysis Entamoeba histolytica D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP isoenzymes are a target for effective control of glucose 6-phosphate formation by energy charge of the cell or by the intracellular level of ATP, ADP and glucose instead of allosteric interconversions of the enzyme Candida maltosa D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP enzyme of the glucose-phosphate branch of the oxidative pentose phosphate pathway Schizosaccharomyces pombe NCYC 132S2-2 D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-glucose + ATP initial enzyme of glycolysis Entamoeba histolytica SFL-3 D-glucose 6-phosphate + ADP - ? 370506 2.7.1.1 D-mannose + ATP - Homo sapiens ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP - Rattus norvegicus ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP - Sus scrofa ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP - Saccharomyces cerevisiae ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP - Aspergillus niger ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP - Lithobates catesbeianus ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP - Trypanosoma cruzi ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP - Candida maltosa ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP - Thermoproteus tenax ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP - Trypanosoma brucei ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP good substrate Rattus norvegicus ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP Hex Ia: 78% of the activity with glucose, Hex Ib: 83% of the activity with glucose, Hex Ic: 85% of the activity with glucose Homo sapiens ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP hexokinase I: 106% of the activity with glucose Homo sapiens ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP hexokinase III: 85% of the activity with glucose Sus scrofa ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP HXK1: good substrate, HXK2: much lower phosphorylation rate than of HXK1 Entamoeba histolytica ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP 82% of the activity with glucose Oryctolagus cuniculus ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP 55% of the activity with glucose Toxoplasma gondii ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP 42% of the activity with fructose Schizosaccharomyces pombe ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP 109% of the activity with glucose, erythrocyte enzyme Oryctolagus cuniculus ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP 23% of the activity with fructose Schizosaccharomyces pombe ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP 104-105% of the activity with glucose Oryctolagus cuniculus ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP 27.2% of the activity with glucose Bubalus bubalis ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP Hex A: 100% of the activity with glucose, Hex B: 98% of the activity with glucose, Hex C: 44% of the activity with glucose Drosophila melanogaster ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP glucokinase Saccharomyces cerevisiae ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP HK1: 53% of the activity with glucose, HK2: 22% of the activity with glucose, HK3: 45% of the activity with glucose Solanum tuberosum ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP 23% of the activity with fructose Schizosaccharomyces pombe PB003 ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP glucokinase Saccharomyces cerevisiae G-517 / CECT 1317 ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP Hex A: 100% of the activity with glucose, Hex B: 98% of the activity with glucose, Hex C: 44% of the activity with glucose Drosophila melanogaster Samarkand ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP 42% of the activity with fructose Schizosaccharomyces pombe PB004 ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 D-mannose + ATP HXK1: good substrate, HXK2: much lower phosphorylation rate than of HXK1 Entamoeba histolytica SFL-3 ADP + D-mannose 6-phosphate - ? 356922 2.7.1.1 GTP + D-glucose - Solanum tuberosum GDP + D-glucose 6-phosphate - ? 356932 2.7.1.1 GTP + D-glucose - Aeropyrum pernix GDP + D-glucose 6-phosphate - ? 356932 2.7.1.1 GTP + D-glucose - Solanum chacoense GDP + D-glucose 6-phosphate - ? 356932 2.7.1.1 GTP + D-glucose - Helianthus annuus GDP + D-glucose 6-phosphate - ? 356932 2.7.1.1 GTP + D-glucose - Clonorchis sinensis GDP + D-glucose 6-phosphate 14% of the activity with ATP ? 356932 2.7.1.1 GTP + D-glucose 52% of the activity with ATP Sulfurisphaera tokodaii GDP + D-glucose 6-phosphate - ? 356932 2.7.1.1 GTP + D-glucose can partially replace ATP Trypanosoma cruzi GDP + D-glucose 6-phosphate - ? 356932 2.7.1.1 GTP + D-glucose 18% of the activity with ATP Thermoproteus tenax GDP + D-glucose 6-phosphate - ? 356932 2.7.1.1 GTP + D-glucose 19% of the activity with ATP Catharanthus roseus GDP + D-glucose 6-phosphate - ? 356932 2.7.1.1 GTP + D-glucose 19% of the activity with ATP Catharanthus roseus B/TH GDP + D-glucose 6-phosphate - ? 356932 2.7.1.1 hexose + ATP - Rattus norvegicus ADP + hexose 6-phosphate - ? 409578 2.7.1.1 ITP + D-glucose - Aeropyrum pernix IDP + D-glucose 6-phosphate - ? 356928 2.7.1.1 ITP + D-glucose - Clonorchis sinensis IDP + D-glucose 6-phosphate 35% of the activity with ATP ? 356928 2.7.1.1 ITP + D-glucose 30% of the activity with ATP Oryctolagus cuniculus IDP + D-glucose 6-phosphate - ? 356928 2.7.1.1 ITP + D-glucose 40% of the activity with ATP Thermoproteus tenax IDP + D-glucose 6-phosphate - ? 356928 2.7.1.1 ITP + D-glucose 12% of the activity with ATP Oryctolagus cuniculus IDP + D-glucose 6-phosphate - ? 356928 2.7.1.1 ITP + D-glucose hexokinase III: 24% of the activity with glucose Sus scrofa IDP + D-glucose 6-phosphate - ? 356928 2.7.1.1 ITP + D-glucose 13.5-14% of the activity with ATP Homo sapiens IDP + D-glucose 6-phosphate - ? 356928 2.7.1.1 ITP + D-glucose 36% of the activity with ATP, erythrocyte enzyme Oryctolagus cuniculus IDP + D-glucose 6-phosphate - ? 356928 2.7.1.1 ITP + D-glucose MgITP Homo sapiens IDP + D-glucose 6-phosphate - ? 356928 2.7.1.1 ITP + D-glucose MgITP Sus scrofa IDP + D-glucose 6-phosphate - ? 356928 2.7.1.1 ITP + D-glucose MgITP Oryctolagus cuniculus IDP + D-glucose 6-phosphate - ? 356928 2.7.1.1 ITP + D-glucose hexokinase I: 9% of the activity with ATP Homo sapiens IDP + D-glucose 6-phosphate - ? 356928 2.7.1.1 ITP + D-glucose 64% of the activity with ATP Sulfurisphaera tokodaii IDP + D-glucose 6-phosphate - ? 356928 2.7.1.1 ITP + D-glucose 64% of the activity with ATP Sulfurisphaera tokodaii 7 IDP + D-glucose 6-phosphate - ? 356928 2.7.1.1 additional information - Sus scrofa ? - ? 89 2.7.1.1 additional information not: ADP Thermoproteus tenax ? - ? 89 2.7.1.1 additional information not: ADP Toxoplasma gondii ? - ? 89 2.7.1.1 additional information ligand binding sites in the N- and C-terminal halves and interactions between these sites Rattus norvegicus ? - ? 89 2.7.1.1 additional information not: UTP Homo sapiens ? - ? 89 2.7.1.1 additional information not: UTP Sus scrofa ? - ? 89 2.7.1.1 additional information not: UTP Oryctolagus cuniculus ? - ? 89 2.7.1.1 additional information not: UTP Ascaris suum ? - ? 89 2.7.1.1 additional information not: UTP Candida maltosa ? - ? 89 2.7.1.1 additional information not: UTP Bubalus bubalis ? - ? 89 2.7.1.1 additional information not: UTP Toxoplasma gondii ? - ? 89 2.7.1.1 additional information structure model of hexokinase I Homo sapiens ? - ? 89 2.7.1.1 additional information structure model of hexokinase I Rattus norvegicus ? - ? 89 2.7.1.1 additional information not: ribose, xylose, phosphorylated hexoses, UDP, polyphosphates Thermoproteus tenax ? - ? 89 2.7.1.1 additional information not: diphosphate Toxoplasma gondii ? - ? 89 2.7.1.1 additional information not: diphosphate Trypanosoma cruzi ? - ? 89 2.7.1.1 additional information glucokinase: not: fructose, galactose, sorbose, rhamnose, arabinose, mannitol, sorbitol, ribose Saccharomyces cerevisiae ? - ? 89 2.7.1.1 additional information not: N-acetyl-D-glucosamine Homo sapiens ? - ? 89 2.7.1.1 additional information not: N-acetyl-D-glucosamine Oryctolagus cuniculus ? - ? 89 2.7.1.1 additional information not: N-acetyl-D-glucosamine Bubalus bubalis ? - ? 89 2.7.1.1 additional information not: mannose Trypanosoma cruzi ? - ? 89 2.7.1.1 additional information not: fructose Entamoeba histolytica ? - ? 89 2.7.1.1 additional information not: fructose Trypanosoma cruzi ? - ? 89 2.7.1.1 additional information role of the hexokinase III carboxyl-domain in determining the catalytic properties of enzyme Homo sapiens ? - ? 89 2.7.1.1 additional information not: D-galactose Homo sapiens ? - ? 89 2.7.1.1 additional information not: D-galactose Rattus norvegicus ? - ? 89 2.7.1.1 additional information not: D-galactose Oryctolagus cuniculus ? - ? 89 2.7.1.1 additional information not: D-galactose Aspergillus niger ? - ? 89 2.7.1.1 additional information not: D-galactose Entamoeba histolytica ? - ? 89 2.7.1.1 additional information not: D-galactose Lithobates catesbeianus ? - ? 89 2.7.1.1 additional information not: D-galactose Bubalus bubalis ? - ? 89 2.7.1.1 additional information not: D-galactose Thermoproteus tenax ? - ? 89 2.7.1.1 additional information not: D-galactose Toxoplasma gondii ? - ? 89 2.7.1.1 additional information not: D-galactose Trypanosoma cruzi ? - ? 89 2.7.1.1 additional information not: ITP Candida maltosa ? - ? 89 2.7.1.1 additional information not: ITP Toxoplasma gondii ? - ? 89 2.7.1.1 additional information not: N-acetylmannosamine, 6-deoxy-6-aminoglucose Saccharomyces cerevisiae ? - ? 89 2.7.1.1 additional information allosteric regulation of hexokinase I, the functional regulatory glucose 6-phosphate binding site is located in the N-terminal half Rattus norvegicus ? - ? 89 2.7.1.1 additional information isolated catalytically active 51 kDa C-fragment and 52 kDa N-fragment without catalytic activity, both fragments contain discrete binding sites for hexoses and hexose 6-phosphates, one of each pair of sites must be latent in the intact enzyme, the regulatory site binding glucose 6-phosphate is associated with the N-terminal half Rattus norvegicus ? - ? 89 2.7.1.1 additional information the 50 kDa C-terminal half of HK I contains the catalytic domain, the N-terminal half is catalytically inactive Homo sapiens ? - ? 89 2.7.1.1 additional information allosteric enzyme, catalyzes phosphoryl transfer from MgATP2- to the 6-OH group of a number of furanose- and pyranose-type compounds Saccharomyces cerevisiae ? - ? 89 2.7.1.1 additional information C-terminal catalytic and N-terminal regulatory domain, detailed structure of hexokinase I, substrate binding sites, models for regulation Homo sapiens ? - ? 89 2.7.1.1 additional information not: trehalose Rattus norvegicus ? - ? 89 2.7.1.1 additional information not: GTP Homo sapiens ? - ? 89 2.7.1.1 additional information not: GTP Sus scrofa ? - ? 89 2.7.1.1 additional information not: GTP Oryctolagus cuniculus ? - ? 89 2.7.1.1 additional information not: GTP Ascaris suum ? - ? 89 2.7.1.1 additional information not: GTP Candida maltosa ? - ? 89 2.7.1.1 additional information not: GTP Bubalus bubalis ? - ? 89 2.7.1.1 additional information not: GTP Toxoplasma gondii ? - ? 89 2.7.1.1 additional information not: CTP Homo sapiens ? - ? 89 2.7.1.1 additional information not: CTP Sus scrofa ? - ? 89 2.7.1.1 additional information not: CTP Oryctolagus cuniculus ? - ? 89 2.7.1.1 additional information not: CTP Bubalus bubalis ? - ? 89 2.7.1.1 additional information not: CTP Thermoproteus tenax ? - ? 89 2.7.1.1 additional information not: CTP Toxoplasma gondii ? - ? 89 2.7.1.1 additional information sperm hexokinase p95/116 may serve as cell surface receptor kinase for the zona pellucida glycoprotein ZP3 Mus musculus ? - ? 89 2.7.1.1 additional information hexokinase PI is a constitutive enzyme, hexokinase PII and glucokinase are regulated by the carbon source used Saccharomyces cerevisiae ? - ? 89 2.7.1.1 additional information loss of enzyme activity is involved in type 2 diabetes mellitus Homo sapiens ? - ? 89 2.7.1.1 additional information the 100 kDa isozymes, displaying internal sequence repetition, may have evolved by gene duplication of a 50 kDa ancestor Mammalia ? - ? 89 2.7.1.1 additional information the enzyme is the only glucose and fructose phosphorylating enzyme, which is upregulating glucose transport and also mediating glucose repression Kluyveromyces lactis ? - ? 89 2.7.1.1 additional information no activity with D-galactose and N-acetylglucosamine, enzyme shows broad substrate specificity, specificity probably depends on the interaction energy occurring by the positional proximity of sugars bound in the active site Thermus caldophilus ? - ? 89 2.7.1.1 additional information no activity with D-xylose, D-galactose, and methyl-alpha-D-glucoside Ogataea angusta ? - ? 89 2.7.1.1 additional information relatively nonspecific enzyme Saccharomyces cerevisiae ? - ? 89 2.7.1.1 additional information the active site is located in the C-terminal domain Bos taurus ? - ? 89 2.7.1.1 additional information glucokinase regulates reproductive function, glucocorticoid secretion, food intake, and hypothalamic gene expression Mus musculus ? - ? 89 2.7.1.1 additional information hexokinase I and hexokinase II are independently regulated, implying that they perform different roles in cardiac glucose regulation Rattus norvegicus ? - ? 89 2.7.1.1 additional information hexokinase, Hxk1, but not the glucokinase, Glk1, is required for normal growth and sugar metabolism, and for pathogenicity on fruits Botrytis cinerea ? - ? 89 2.7.1.1 additional information OsHXK2 expression appears to be significantly upregulated in rice leaves by glucose and fructose Oryza sativa ? - ? 89 2.7.1.1 additional information OsHXK5 expression appears to be significantly upregulated in rice leaves by glucose and fructose Oryza sativa ? - ? 89 2.7.1.1 additional information OsHXK6 expression appeared to be significantly upregulated in rice leaves by glucose and fructose Oryza sativa ? - ? 89 2.7.1.1 additional information OsHXK8 may function in the endosperm in the filling phase of rice seed development Oryza sativa ? - ? 89 2.7.1.1 additional information role of a bound glucokinase protein fraction in the regulation of insulin granule movement along tubulin filaments Mus musculus ? - ? 89 2.7.1.1 additional information the enzyme plays a role in glycogen synthesis and glucose homeostasis Rattus norvegicus ? - ? 89 2.7.1.1 additional information the C-terminal tail of TbHK1 is important for hexokinase activity Trypanosoma brucei ? - ? 89 2.7.1.1 additional information G/F discrepancy in Saccharomyces cerevisiae is not related to the maximal hexokinase activity in vitro with glucose or fructose as substrate or to the ratio between the Km values for glucose and fructose phosphorylation but might rather be related to the actual fructose phosphorylating activity at physiological fructose levels in vivo Saccharomyces cerevisiae ? - ? 89 2.7.1.1 additional information biophysical characterization of the interaction between GK and GKRP and its modulation by physiological and pharmacological effectors Homo sapiens ? - ? 89 2.7.1.1 additional information galactose is phosphorylated at rates of 0.8 and 0.08 U/mg at concentrations of 1 and 10 mM, respectively. Ribose, xylose, N-acetylmannosamine, glucose-1-phosphate, and fructose-6-phosphate (10 mM) are not phosphorylated by the enzyme Sulfurisphaera tokodaii ? - ? 89 2.7.1.1 additional information no substrate: D-galactose Eimeria tenella ? - ? 89 2.7.1.1 additional information no substrates: D-galactose, sorbose. TTP, UTP, CTP and GTP show 6.5%-17.1% of the activity with ATP Cryptosporidium parvum ? - ? 89 2.7.1.1 additional information the enzyme shows little catalytic capacity for galactose and fructose Thermus thermophilus ? - ? 89 2.7.1.1 additional information not: ADP Toxoplasma gondii RH ? - ? 89 2.7.1.1 additional information not: UTP Toxoplasma gondii RH ? - ? 89 2.7.1.1 additional information not: diphosphate Toxoplasma gondii RH ? - ? 89 2.7.1.1 additional information not: D-galactose Toxoplasma gondii RH ? - ? 89 2.7.1.1 additional information not: ITP Toxoplasma gondii RH ? - ? 89 2.7.1.1 additional information galactose is phosphorylated at rates of 0.8 and 0.08 U/mg at concentrations of 1 and 10 mM, respectively. Ribose, xylose, N-acetylmannosamine, glucose-1-phosphate, and fructose-6-phosphate (10 mM) are not phosphorylated by the enzyme Sulfurisphaera tokodaii 7 ? - ? 89 2.7.1.1 additional information glucokinase: not: fructose, galactose, sorbose, rhamnose, arabinose, mannitol, sorbitol, ribose Saccharomyces cerevisiae G-517 / CECT 1317 ? - ? 89 2.7.1.1 additional information hexokinase PI is a constitutive enzyme, hexokinase PII and glucokinase are regulated by the carbon source used Saccharomyces cerevisiae G-517 / CECT 1317 ? - ? 89 2.7.1.1 additional information the enzyme shows little catalytic capacity for galactose and fructose Thermus thermophilus DSM 579 ? - ? 89 2.7.1.1 additional information no activity with D-galactose and N-acetylglucosamine, enzyme shows broad substrate specificity, specificity probably depends on the interaction energy occurring by the positional proximity of sugars bound in the active site Thermus caldophilus GK24 ? - ? 89 2.7.1.1 additional information not: fructose Entamoeba histolytica SFL-3 ? - ? 89 2.7.1.1 additional information not: D-galactose Entamoeba histolytica SFL-3 ? - ? 89 2.7.1.1 additional information no substrates: D-galactose, sorbose. TTP, UTP, CTP and GTP show 6.5%-17.1% of the activity with ATP Cryptosporidium parvum Iowa II ? - ? 89 2.7.1.1 N-acetyl-D-glucosamine + ATP erythrocyte enzyme, 32.6% of the activity with glucose Oryctolagus cuniculus ADP + N-acetyl-D-glucosamine 6-phosphate - ? 356926 2.7.1.1 TTP + D-glucose - Solanum chacoense TDP + D-glucose 6-phosphate - ? 389934 2.7.1.1 TTP + D-glucose - Clonorchis sinensis TDP + D-glucose 6-phosphate 21% of the activity with ATP ? 389934 2.7.1.1 UTP + D-glucose - Solanum tuberosum UDP + D-glucose 6-phosphate - ? 356931 2.7.1.1 UTP + D-glucose - Aeropyrum pernix UDP + D-glucose 6-phosphate - ? 356931 2.7.1.1 UTP + D-glucose - Solanum chacoense UDP + D-glucose 6-phosphate - ? 356931 2.7.1.1 UTP + D-glucose - Helianthus annuus UDP + D-glucose 6-phosphate - ? 356931 2.7.1.1 UTP + D-glucose - Clonorchis sinensis UDP + D-glucose 6-phosphate 13% of the activity with ATP ? 356931 2.7.1.1 UTP + D-glucose 17% of the activity with ATP Thermoproteus tenax UDP + D-glucose 6-phosphate - ? 356931 2.7.1.1 UTP + D-glucose 14% of the activity with ATP Oryctolagus cuniculus UDP + D-glucose 6-phosphate - ? 356931 2.7.1.1 UTP + D-glucose 37% of the activity with ATP Sulfurisphaera tokodaii UDP + D-glucose 6-phosphate - ? 356931 2.7.1.1 UTP + D-glucose can partially replace ATP Trypanosoma cruzi UDP + D-glucose 6-phosphate - ? 356931 2.7.1.1 UTP + D-glucose 23% of the activity with ATP Catharanthus roseus UDP + D-glucose 6-phosphate - ? 356931 2.7.1.1 UTP + D-glucose hexokinase I: 5% of the activity with ATP Homo sapiens UDP + D-glucose 6-phosphate - ? 356931 2.7.1.1 UTP + D-glucose MgUTP Homo sapiens UDP + D-glucose 6-phosphate - ? 356931 2.7.1.1 UTP + D-glucose MgUTP Oryctolagus cuniculus UDP + D-glucose 6-phosphate - ? 356931 2.7.1.1 UTP + D-glucose 23% of the activity with ATP Catharanthus roseus B/TH UDP + D-glucose 6-phosphate - ? 356931