2.5.1.47 2 L-cysteine - Lactiplantibacillus plantarum (2R,2'R)-3,3'-sulfanediylbis(2-aminopropanoic acid) + H2S - ? 444602 2.5.1.47 3-chloro-L-alanine + NaHS beta-replacement reaction, enzyme can be induced by 3-chloro-L-alanine Lysinibacillus sphaericus L-cysteine + ? - ? 354304 2.5.1.47 5-thio-2-nitrobenzoate + O-acetyl-L-serine - Salmonella enterica subsp. enterica serovar Typhimurium acetate + ? - ? 408212 2.5.1.47 beta-chloro-L-alanine + 2-nitro-5-thiobenzoate - Geobacillus stearothermophilus ? - ? 379549 2.5.1.47 chloroalanine + sulfide beta-chloroalanine, 6% of the activity with O-acetyl-L-Ser Thermus thermophilus cysteine + chloride - r 354309 2.5.1.47 chloroalanine + sulfide 3-6% of the activity of the cysteine synthase reaction Spinacia oleracea cysteine + chloride - r 354309 2.5.1.47 chloroalanine + sulfide beta-chloroalanine, 6% of the activity with O-acetyl-L-Ser Thermus thermophilus HB8 / ATCC 27634 / DSM 579 cysteine + chloride - r 354309 2.5.1.47 cyanide + cysteine - Solanum tuberosum beta-cyanoalanine + sulfide - ? 354315 2.5.1.47 cysteine + CN- - Xanthium pennsylvanicum cyanoalanine + H2S - ? 354307 2.5.1.47 cysteine + CN- - Spinacia oleracea cyanoalanine + H2S H2S i.e. bisulfide ? 354307 2.5.1.47 cysteine + CN- involved in cyanide metabolism during seed germination Xanthium pennsylvanicum cyanoalanine + H2S - ? 354307 2.5.1.47 L-Cys + acetate - Salmonella enterica subsp. enterica serovar Typhimurium O-acetyl-L-Ser + H2S - r 354297 2.5.1.47 L-Cys + acetate - Escherichia coli O-acetyl-L-Ser + H2S - r 354297 2.5.1.47 L-Cys + acetate equilibrium constant Salmonella enterica subsp. enterica serovar Typhimurium O-acetyl-L-Ser + H2S - r 354297 2.5.1.47 L-Cys + acetate involved in mobilization of sulfide from cysteine for Fe-S cluster formation, significance in vivo unclear Escherichia coli ? - ? 370434 2.5.1.47 L-Cys + dithiothreitol - Lacticaseibacillus casei beta-cyanoalanine + H2S - ? 423732 2.5.1.47 L-cysteine + cyanide - Geobacillus stearothermophilus cyanoalanine + H2S - ? 379981 2.5.1.47 L-cysteine + dithiothreitol - Arabidopsis sp. S-(2,3-hydroxy-4-thiobutyl)-L-cysteine + H2S - ? 354314 2.5.1.47 L-cysteine + dithiothreitol - Aeropyrum pernix S-(2,3-hydroxy-4-thiobutyl)-L-cysteine + H2S - ? 354314 2.5.1.47 L-cysteine + dithiothreitol the side reaction of the enzyme seems to contribute massively to the total H2S release of higher plants at least at higher pH values Arabidopsis sp. S-(2,3-hydroxy-4-thiobutyl)-L-cysteine + H2S - ? 354314 2.5.1.47 L-cysteine + H2O - Bacillus anthracis L-serine + H2S - ? 445593 2.5.1.47 L-cysteine + L-cysteine preferred reaction Fusobacterium nucleatum subsp. nucleatum L-lanthionine + H2S - ? 412247 2.5.1.47 L-cysteine + L-cysteine preferred reaction Fusobacterium nucleatum subsp. nucleatum ATCC 25586 L-lanthionine + H2S - ? 412247 2.5.1.47 L-cysteine + L-homocysteine - Bacillus anthracis L-cystathionine + H2S - ? 409645 2.5.1.47 L-cysteine + L-homocysteine - Lactiplantibacillus plantarum L-cystathionine + H2S - ? 409645 2.5.1.47 L-homocysteine + L-serine - Aeropyrum pernix L-cystathionine + H2O - ? 354316 2.5.1.47 L-homoserine + sulfide 1.6% of the activity with O-acetyl-L-serine Thermus thermophilus ? - ? 354312 2.5.1.47 monofluoralanine + H2S - Salmonella enterica subsp. enterica serovar Typhimurium ? - ? 458135 2.5.1.47 additional information isoenzyme 1 and 2 have different substrate specificities towards various beta-substituted L-Cys Allium tuberosum ? - ? 89 2.5.1.47 additional information several nucleophiles may stimulate sulfide formation Escherichia coli ? - ? 89 2.5.1.47 additional information activity of the enzyme bound to serine acetyltransferase is lower than that of the free enzyme Escherichia coli ? - ? 89 2.5.1.47 additional information beta-substituted alanines, low activity Spinacia oleracea ? - ? 89 2.5.1.47 additional information beta-substituted alanines, low activity Citrullus lanatus subsp. vulgaris ? - ? 89 2.5.1.47 additional information beta-substituted alanines, low activity Brassica juncea ? - ? 89 2.5.1.47 additional information enzyme is induced in leaves exposed to salt stress. The results suggest that the plant enzyme is responding to the salt stress by inducing cysteine biosynthesis as a protection against high ion concentrations Arabidopsis thaliana ? - ? 89 2.5.1.47 additional information model of a dynamic cysteine synthesis system with regulatory function Arabidopsis thaliana ? - ? 89 2.5.1.47 additional information no substrate: L-serine, L-homoserine, O-acetyl-L-homoserine, L-alanine, L-homocysteine Geobacillus stearothermophilus ? - ? 89 2.5.1.47 additional information no substrate: L-serine, O-propionyl-L-serine, L-alanine, glycine Geobacillus stearothermophilus ? - ? 89 2.5.1.47 additional information preferred state of sulfide is hydrogen sulfide Arabidopsis thaliana ? - ? 89 2.5.1.47 additional information the enzyme is induced by Al3+. Cysteine synthase may be a key player during Al response/adaptation in rice Oryza sativa ? - ? 89 2.5.1.47 additional information the enzyme is involved in tellurite resistance. OASS is not essential for cysteine biosynthesis Azospirillum brasilense ? - ? 89 2.5.1.47 additional information cysteine synthase CysB is the only isoform of physiological importance in Aspergillus nidulans. Starvation-induced cysteine synthase activity is under control of cross-pathway regulation Aspergillus nidulans ? - ? 89 2.5.1.47 additional information stopped-flow fluorescence spectroscopy is used to characterize the interaction of serine acetyltransferase with OASS and in the presence of the physiological regulators cysteine and bisulfide. Cysteine synthase assembly occurs via a two-step mechanism involving rapid formation of an encounter complex between the two enzymes, followed by a slow conformational change. The conformational change likely results from the closure of the active site of OASS upon binding of the serine acetyltransferase C-terminal peptide. Bisulfide stabilizes the cysteine synthase complex mainly by decreasing the back rate of the isomerization step. Cysteine, the product of the OASS reaction and a SAT inhibitor, slightly affects the kinetics of cysteine synthase formation leading to destabilization of the complex Haemophilus influenzae ? - ? 89 2.5.1.47 additional information the binding free energy of 400 pentapeptides, MNXXI, interacting with the HiOASS-A active site using a combined docking-scoring procedure based on GOLD and HINTare examined. The free energy prediction is verified by the experimental determination of the binding affinity of 14 of these pentapeptides, selected for spanning a large range of predicted binding affinity and presenting charged, polar, or apolar residues at mutation sites Haemophilus influenzae ? - ? 89 2.5.1.47 additional information no substrates: serine, phosphoserine, O-succinylhomoserine, thiosulfate Lacticaseibacillus casei ? - ? 89 2.5.1.47 additional information the enzyme also catalyzes the reaction of EC 2.5.1.65, O-phosphoserine hydrolase Aeropyrum pernix ? - ? 89 2.5.1.47 additional information the enzyme shows H2S synthesizing activity, cysteine synthase activity and also L-3-cyanoalanine synthase activity, EC 4.4.1.9 Caenorhabditis elegans ? - ? 89 2.5.1.47 additional information the mitochondrial isozyme OAS-TL C accounts for less than 5% of total OAS-TL activity Arabidopsis thaliana ? - ? 89 2.5.1.47 additional information enzyme additionally catalyzes the formation of O-ureido-L-serine from O-acetyl-L-serine and hydroxyurea, reaction of EC 2.6.99.3. The kcat/Km value of DcsD for L-cysteine synthesis is 80fold higher than that for O-ureido-L-serine synthesis Streptomyces lavendulae ? - ? 89 2.5.1.47 additional information no synthesis of mimosine. The enzyme is specific for cysteine synthesis. The recombinant enzyme is active with or without the leader peptide Leucaena leucocephala ? - ? 89 2.5.1.47 additional information no substrate: L-serine Bacillus anthracis ? - ? 89 2.5.1.47 additional information enzyme additionally catalyzes synthesis of mimosine, reaction of EC 2.5.1.52. The apparent kcat for Cys production is over sixfold higher than mimosine synthesis and the apparent Km is 3.7 times lower Leucaena leucocephala ? - - 89 2.5.1.47 additional information enzyme is specific for synthesis of cysteine, no synthesis of mimosine Mimosa pudica ? - - 89 2.5.1.47 additional information enzyme is unable to synthesize mimosine, reaction of EC 2.5.1.52 Leucaena leucocephala ? - - 89 2.5.1.47 additional information protein predominately catalyzes the synthesis but not the degradation of cysteine. The L-cysteine desulfhydrase reaction may be a side reaction Oryza sativa Japonica Group ? - - 89 2.5.1.47 additional information the enzyme is involved in tellurite resistance. OASS is not essential for cysteine biosynthesis Azospirillum brasilense Sp7 ? - ? 89 2.5.1.47 additional information enzyme additionally catalyzes the formation of O-ureido-L-serine from O-acetyl-L-serine and hydroxyurea, reaction of EC 2.6.99.3. The kcat/Km value of DcsD for L-cysteine synthesis is 80fold higher than that for O-ureido-L-serine synthesis Streptomyces lavendulae ATCC 11924 ? - ? 89 2.5.1.47 NaN3 + O-acetyl-Ser - Escherichia coli beta-azidoalanine + sodium acetate mutagenic ? 354302 2.5.1.47 NaN3 + O-acetyl-Ser - Hordeum vulgare beta-azidoalanine + sodium acetate mutagenic in Salmonella typhimurium ? 354302 2.5.1.47 O-acetyl-L-Ser + 1,2,3,4-tetrazole - Escherichia coli ? - ? 389409 2.5.1.47 O-acetyl-L-Ser + 1,2,3-benzotriazole weak activity Escherichia coli ? - ? 389410 2.5.1.47 O-acetyl-L-Ser + 1,2,4-triazole - Escherichia coli ? - ? 389411 2.5.1.47 O-acetyl-L-Ser + 1-propanethiol - Escherichia coli ? - ? 389412 2.5.1.47 O-acetyl-L-Ser + 2-propene-1-thiol 6.2% of the activity with sulfide, isoenzyme 2 Citrullus lanatus subsp. vulgaris S-allyl-L-cysteine + ? - ? 354305 2.5.1.47 O-acetyl-L-Ser + 2-propene-1-thiol 2.6% and 6.8% of the activity with sulfide, isoenzyme 1 and 2, respectively Pisum sativum S-allyl-L-cysteine + ? - ? 354305 2.5.1.47 O-acetyl-L-Ser + 2-propene-1-thiol 18% of activity with sulfide Spinacia oleracea S-allyl-L-cysteine + ? - ? 354305 2.5.1.47 O-acetyl-L-Ser + 2-propene-1-thiol 18% of activity with sulfide Brassica juncea S-allyl-L-cysteine + ? - ? 354305 2.5.1.47 O-acetyl-L-Ser + 3-mercapto-1,2,4-triazole - Escherichia coli ? - ? 389413 2.5.1.47 O-acetyl-L-Ser + 5-mercapto-2-nitrobenzoate - Salmonella enterica subsp. enterica serovar Typhimurium S-(3-carboxy-4-nitrophenyl)-L-cysteine + ? - ? 354308 2.5.1.47 O-acetyl-L-Ser + benzenethiol - Escherichia coli L-cys + benzylacetate - ? 389414 2.5.1.47 O-acetyl-L-Ser + cysteamine weak activity Escherichia coli ? - ? 389415 2.5.1.47 O-acetyl-L-Ser + H2S - Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate - r 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Thermus thermophilus L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Escherichia coli L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Escherichia coli L-Cys + acetate - r 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Spinacia oleracea L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Spinacia oleracea L-Cys + acetate - r 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Pisum sativum L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Solanum tuberosum L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Arabidopsis thaliana L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Trichomonas vaginalis L-Cys + acetate - r 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Arabidopsis sp. L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Mycobacterium tuberculosis L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Oryza sativa L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Enterobacter sp. L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Citrullus lanatus subsp. vulgaris L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Methanosarcina thermophila L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Brassica juncea L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Aeropyrum pernix L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Lacticaseibacillus casei L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Oryza sativa Japonica Group L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Leishmania donovani L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S equilibrium constant Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate - r 354296 2.5.1.47 O-acetyl-L-Ser + H2S Entamoeba histolytica, the causative agent of human amoebiasis, is essentially anaerobic, requiring a small amount of oxygen for growth. It cannot tolerate the higher concentration of oxygen present in human tissues or blood. However, during tissue invasion it is exposed to a higher level of oxygen, leading to oxygen stress. Cysteine, which is a vital thiol in Entamoeba histolytica, plays an essential role in its oxygen-defence mechanisms. The major route of cysteine biosynthesis in this parasite is the condensation of O-acetylserine with sulfide by the de novo cysteine-biosynthetic pathway, which involves cysteine synthase (EhCS) as a key enzyme Entamoeba histolytica L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S enzyme that catalyzes the final step in cysteine biosynthesis. Cysteine synthetase is a global regulator of the expression of genes involved in sulfur assimilation Bacillus subtilis L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S OASTL activity regulates not only Cys de novo synthesis but also its homeostasis Solanum tuberosum L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S the second half of the OASS-A reaction is limited by the conformational change needed to open the active site and release the amino acid product. No quinonoid or geminal-diamine intermediates are detected. The amino acid external Schiff base of the enzyme is found to be very stable when the reaction is run in D2O Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S yeast two-hybrid system for screening of a cDNA library of Nicotiana plumbaginifolia for clones encoding plant proteins interacting with two proteins of Escherichia coli: serine acetyltransferase (SAT, the product of cysE gene) and O-acetylserine (thiol) lyase A, also termed cysteine synthase (OASTL-A, the product of cysK gene). Two plant cDNA clones are identified when using the cysE gene as a bait Nicotiana plumbaginifolia L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Enterobacter sp. 10-1 L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + H2S - Thermus thermophilus HB8 / ATCC 27634 / DSM 579 L-Cys + acetate - ? 354296 2.5.1.47 O-acetyl-L-Ser + hydrogen sulfide - Leucaena leucocephala L-Cys + acetate - ? 458172 2.5.1.47 O-acetyl-L-Ser + hydrogen sulfide - Oryza sativa Japonica Group L-Cys + acetate - ? 458172 2.5.1.47 O-acetyl-L-Ser + hydrogen sulfide - Mimosa pudica L-Cys + acetate - ? 458172 2.5.1.47 O-acetyl-L-Ser + isoxazolin-5-one synthesis of precursor of neurotoxin beta-N-oxalyl-L-alpha,beta-diaminopropionic acid Lathyrus sativus ? - ? 370435 2.5.1.47 O-acetyl-L-Ser + isoxazolin-5-one synthesis of precursor of neurotoxin beta-N-oxalyl-L-alpha,beta-diaminopropionic acid Lathyrus odoratus ? - ? 370435 2.5.1.47 O-acetyl-L-Ser + mercaptoacetic acid 2.2% of activity with sulfide, isoenzyme 1 and 2 Citrullus lanatus subsp. vulgaris S-carboxymethyl-L-cysteine - ? 354306 2.5.1.47 O-acetyl-L-Ser + mercaptoacetic acid 2.3% and 1.6% of activity with sulfide, isoenzymes 1 and 2, respectively Pisum sativum S-carboxymethyl-L-cysteine - ? 354306 2.5.1.47 O-acetyl-L-Ser + mercaptoacetic acid 6.1% of activity with sulfide Brassica juncea S-carboxymethyl-L-cysteine - ? 354306 2.5.1.47 O-acetyl-L-Ser + mercaptoacetic acid 2.5% of activity with sulfide Spinacia oleracea S-carboxymethyl-L-cysteine - ? 354306 2.5.1.47 O-acetyl-L-Ser + methyl mercaptan - Salmonella enterica subsp. enterica serovar Typhimurium S-methylcysteine + acetate - ? 354299 2.5.1.47 O-acetyl-L-Ser + methyl mercaptan - Saccharomyces cerevisiae S-methylcysteine + acetate - ir 354299 2.5.1.47 O-acetyl-L-Ser + methyl mercaptan - Neurospora crassa S-methylcysteine + acetate - ir 354299 2.5.1.47 O-acetyl-L-Ser + methyl mercaptan - Brassica rapa S-methylcysteine + acetate - ir 354299 2.5.1.47 O-acetyl-L-Ser + methyl mercaptan - Salmonella enterica subsp. enterica serovar Typhimurium S-methylcysteine + acetate product identification uncertain ir 354299 2.5.1.47 O-acetyl-L-Ser + methyl mercaptan 4% and 1% of activity with sulfide, isoenzymes 1 and 2, respectively Pisum sativum S-methylcysteine + acetate - ? 354299 2.5.1.47 O-acetyl-L-Ser + methyl mercaptan 21.5% and 77% of activity with sulfide, isoenzymes 1 and 2, respectively Citrullus lanatus subsp. vulgaris S-methylcysteine + acetate - ? 354299 2.5.1.47 O-acetyl-L-Ser + methyl mercaptan 32% of activity with sulfide Spinacia oleracea S-methylcysteine + acetate - ? 354299 2.5.1.47 O-acetyl-L-Ser + NaCN - Enterobacter sp. beta-cyanoalanine + sodium acetate - ? 354301 2.5.1.47 O-acetyl-L-Ser + NaCN 6.84% and 7.64% of activity with sulfide, isoenzymes 1 and 2, respectively Allium tuberosum beta-cyanoalanine + sodium acetate - ? 354301 2.5.1.47 O-acetyl-L-Ser + NaCN 12.3% of activity with sulfide Brassica juncea beta-cyanoalanine + sodium acetate - ? 354301 2.5.1.47 O-acetyl-L-Ser + NaCN - Enterobacter sp. 10-1 beta-cyanoalanine + sodium acetate - ? 354301 2.5.1.47 O-acetyl-L-Ser + pyrazole weak activity Escherichia coli ? - ? 389416 2.5.1.47 O-acetyl-L-Ser + S2O32- - Escherichia coli S-sulfocysteine + ? - ? 424947 2.5.1.47 O-acetyl-L-Ser + S2O32- - Escherichia coli BW25113 S-sulfocysteine + ? - ? 424947 2.5.1.47 O-acetyl-L-Ser + sodium azide weak activity Escherichia coli ? - ? 389417 2.5.1.47 O-acetyl-L-Ser + sodium thiosulfate - Staphylococcus aureus ? - ? 389418 2.5.1.47 O-acetyl-L-Ser + sodium thiosulfate - Escherichia coli ? - ? 389418 2.5.1.47 O-acetyl-L-Ser + sodium thiosulfate - Staphylococcus aureus SH1000 ? - ? 389418 2.5.1.47 O-acetyl-L-Ser + sulfide - Escherichia coli L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide - Saccharomyces cerevisiae L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide - Neurospora crassa L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide - Arabidopsis thaliana L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide - Brassica rapa L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide the cysteine synthase complex functions as a molecular sensor system that monitors the sulfur status of the cell and controls sulfate assimilation and cysteine synthesis according to the availability of sulfate Arabidopsis thaliana L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide key role in metabolism of S-containing amino acids Saccharomyces cerevisiae L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide activity varies between sulfur sources, enzyme formation regulated by L-Cys concentration [Chlorella] fusca L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide involved in synthesis of antioxidants such as glutathione during fruit development Capsicum annuum L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide involved in glutathione formation Datura inoxia L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide enzyme transcription repressed by L-cystine, derepressed by limiting sulfide concentrations Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide functions as a Cys synthase rather than as a homocysteine synthase in vivo Saccharomyces cerevisiae L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide controlled by feedback inhibition, adaptively significant as sulfide removal mechanism Paracoccus denitrificans L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide repressed during growth with sulfide or thiosulfide as sulfur source Rhodocyclus tenuis L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide involved in thiosulfate assimilation Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide final step in Cys synthesis Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide final step in Cys synthesis Escherichia coli L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide final step in Cys synthesis Spinacia oleracea L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide final step in Cys synthesis Raphanus sativus L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide final step in Cys synthesis Methanosarcina thermophila L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide last step of assimilatory sulfate reduction Spinacia oleracea L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide last step of assimilatory sulfate reduction Arabidopsis thaliana L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide last step of assimilatory sulfate reduction [Chlorella] fusca L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide last step of assimilatory sulfate reduction Synechococcus sp. L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + sulfide last step of assimilatory sulfate reduction Synechococcus sp. 6301 L-Cys + acetate - ? 370432 2.5.1.47 O-acetyl-L-Ser + thiosulfate - Salmonella enterica subsp. enterica serovar Typhimurium S-sulfocysteine + sodium acetate - ? 354303 2.5.1.47 O-acetyl-L-Ser + thiosulfate - Aeropyrum pernix S-sulfocysteine + sodium acetate - ? 354303 2.5.1.47 O-acetyl-L-serine - Salmonella enterica subsp. enterica serovar Typhimurium L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Paracoccus denitrificans L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Chlamydomonas reinhardtii L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Thermus thermophilus L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Escherichia coli L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Triticum aestivum L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Penicillium chrysogenum L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Spinacia oleracea L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Pisum sativum L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Solanum tuberosum L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Arabidopsis thaliana L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Trichomonas vaginalis L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Capsicum annuum L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Synechococcus sp. L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Allochromatium vinosum L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Enterobacter sp. L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Trifolium repens L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Citrullus lanatus subsp. vulgaris L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Methanosarcina thermophila L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Brassica juncea L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Xanthium pennsylvanicum L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Aeropyrum pernix L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Rhodocyclus tenuis L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Streptomyces lavendulae L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Fusobacterium nucleatum subsp. nucleatum L-cysteine + acetate - ir 380143 2.5.1.47 O-acetyl-L-serine - Bacillus anthracis L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Lactiplantibacillus plantarum L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Fusobacterium nucleatum subsp. nucleatum ATCC 25586 L-cysteine + acetate - ir 380143 2.5.1.47 O-acetyl-L-serine - Enterobacter sp. 10-1 L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Synechococcus sp. 6301 L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine - Streptomyces lavendulae ATCC 11924 L-cysteine + acetate - ? 380143 2.5.1.47 O-acetyl-L-serine + 5-thio-2-nitrobenzoate - Salmonella enterica subsp. enterica serovar Typhimurium ? + acetate - ? 397994 2.5.1.47 O-acetyl-L-serine + 5-thio-2-nitrobenzoate - Escherichia coli ? + acetate - ? 397994 2.5.1.47 O-acetyl-L-serine + 5-thio-2-nitrobenzoate - Salmonella enterica subsp. enterica serovar Typhimurium DW378 ? + acetate - ? 397994 2.5.1.47 O-acetyl-L-serine + H2O - Lactiplantibacillus plantarum pyruvate + acetate + NH3 - ? 445760 2.5.1.47 O-acetyl-L-serine + H2S - Bacillus anthracis L-cysteine + acetic acid - ? 354678 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Staphylococcus aureus L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Escherichia coli L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Chlorella sorokiniana L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Salmonella enterica subsp. enterica serovar Typhimurium L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Glycine max L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Haemophilus influenzae L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Arabidopsis thaliana L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Mycobacterium tuberculosis L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Caenorhabditis elegans L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Leishmania braziliensis L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Leucaena leucocephala L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Brucella abortus L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Bacillus subtilis L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Entamoeba histolytica L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Microcystis aeruginosa L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Lathyrus sativus L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate. The enzyme catalyzes the reaction with O-acetyl-L-serine and hydroxyurea with 80fold lower catalytic efficiency Streptomyces lavendulae L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide residue Arg210 near the entrance of the active site and is important for O-acetyl-L-serine substrate recognition Escherichia coli L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Leishmania braziliensis MHOM/BR/75/M2904 L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Chlorella sorokiniana 211/8 K L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide - Escherichia coli W3110 / ATCC 27325 L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide residue Arg210 near the entrance of the active site and is important for O-acetyl-L-serine substrate recognition Escherichia coli W3110 / ATCC 27325 L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + hydrogen sulfide the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate. The enzyme catalyzes the reaction with O-acetyl-L-serine and hydroxyurea with 80fold lower catalytic efficiency Streptomyces lavendulae ATCC 11924 L-cysteine + acetate - ? 375218 2.5.1.47 O-acetyl-L-serine + L-homocysteine - Lactiplantibacillus plantarum cystathionine + acetate - ? 423903 2.5.1.47 O-acetyl-L-serine + L-homocysteine the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate Streptomyces lavendulae cystathionine + acetate - ? 423903 2.5.1.47 O-acetyl-L-serine + L-homocysteine the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate Streptomyces lavendulae ATCC 11924 cystathionine + acetate - ? 423903 2.5.1.47 O-acetyl-L-serine + sulfide - Glycine max L-Cys + acetate - ? 409985 2.5.1.47 O-acetyl-L-serine + thiosulfate - Arabidopsis thaliana S-sulfo-L-cysteine + acetate - ? 409986 2.5.1.47 O-acetyl-L-serine + thiosulfate the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate Streptomyces lavendulae S-sulfo-L-cysteine + acetate - ? 409986 2.5.1.47 O-acetyl-L-serine + thiosulfate the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate Streptomyces lavendulae ATCC 11924 S-sulfo-L-cysteine + acetate - ? 409986 2.5.1.47 O-acetyl-L-serine + thiosulfate - Salmonella enterica subsp. enterica serovar Typhimurium S-sulfo-L-cysteine + acetate + H+ - ? 423905 2.5.1.47 O-acetyl-Ser + selenide maximal 40% rate of cysteine synthesis Paracoccus denitrificans selenocysteine + acetate - ? 354300 2.5.1.47 O-acetylhomoserine + H2S - Saccharomyces cerevisiae homocysteine + ? - ? 354298 2.5.1.47 O-acetylhomoserine + H2S not, low molecular weight enzyme Saccharomyces cerevisiae homocysteine + ? - ? 354298 2.5.1.47 O-acetylhomoserine + H2S 2.4% of the activity with O-acetyl-L-Ser Thermus thermophilus homocysteine + ? - ? 354298 2.5.1.47 O-acetylhomoserine + H2S 2.4% of the activity with O-acetyl-L-Ser Thermus thermophilus HB8 / ATCC 27634 / DSM 579 homocysteine + ? - ? 354298 2.5.1.47 O-diazoacetyl-L-serine + sulfide 44% of the activity with O-acetyl-Ser Thermus thermophilus ? - ? 354310 2.5.1.47 O-diazoacetyl-L-serine + sulfide 44% of the activity with O-acetyl-Ser Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ? - ? 354310 2.5.1.47 O-phosphoserine + H2S - Trichomonas vaginalis L-Cys + phosphate - ? 389428 2.5.1.47 O-succinyl-L-homoserine + sulfide 3.6% of the activity with O-acetyl-L-serine Thermus thermophilus ? - ? 354311 2.5.1.47 O-succinyl-L-homoserine + sulfide 3.6% of the activity with O-acetyl-L-serine Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ? - ? 354311 2.5.1.47 O3-acetyl-L-serine - Geobacillus stearothermophilus alpha-aminoacrylate in absence of S2- and at 50°C, not below ? 380162 2.5.1.47 O3-acetyl-L-serine + 2-nitro-5-thiobenzoate - Salmonella enterica subsp. enterica serovar Typhimurium ? - ? 380151 2.5.1.47 O3-acetyl-L-serine + 2-nitro-5-thiobenzoate - Geobacillus stearothermophilus ? - ? 380151 2.5.1.47 O3-acetyl-L-serine + 5-thio-2-nitrobenzoate - Salmonella enterica subsp. enterica serovar Typhimurium ? + acetate - ? 410924 2.5.1.47 O3-acetyl-L-serine + 5-thio-2-nitrobenzoate - Salmonella enterica subsp. enterica serovar Typhimurium DW378 ? + acetate - ? 410924 2.5.1.47 O3-acetyl-L-serine + benzylmercaptan - Geobacillus stearothermophilus S-benzyl-L-cysteine + acetate - ? 380152 2.5.1.47 O3-acetyl-L-serine + cyanide - Geobacillus stearothermophilus beta-cyano-L-alanine + acetate - ? 380153 2.5.1.47 O3-acetyl-L-serine + ethylmercaptan - Geobacillus stearothermophilus S-ethyl-L-cysteine + acetate - ? 380154 2.5.1.47 O3-acetyl-L-serine + hydrogen sulfide - Salmonella enterica subsp. enterica serovar Typhimurium L-cysteine + acetate - ? 380155 2.5.1.47 O3-acetyl-L-serine + hydrogen sulfide - Arabidopsis thaliana L-cysteine + acetate - ? 380155 2.5.1.47 O3-acetyl-L-serine + hydrogen sulfide - Acidithiobacillus ferrooxidans ATCC 23270 L-cysteine + acetate - ? 380155 2.5.1.47 O3-acetyl-L-serine + hydrogen sulfide - Haemophilus influenzae L-cysteine + acetate - ? 380155 2.5.1.47 O3-acetyl-L-serine + hydrogen sulfide - Aeropyrum pernix L-cysteine + acetate - ? 380155 2.5.1.47 O3-acetyl-L-serine + hydrogen sulfide 970% of the activity with cyanide Geobacillus stearothermophilus L-cysteine + acetate - ? 380155 2.5.1.47 O3-acetyl-L-serine + hydrogen sulfide in the absence of sulfide O3-acetyl-L-serine reacts with the cofactor pyridoxal 5'-phosphate to alpha-aminoacrylate intermediate Entamoeba histolytica L-cysteine + acetate - ? 380155 2.5.1.47 O3-acetyl-L-serine + hydrogen sulfide - Salmonella enterica subsp. enterica serovar Typhimurium DW378 L-cysteine + acetate - ? 380155 2.5.1.47 O3-acetyl-L-serine + hydrogensulfide - Arabidopsis thaliana L-cysteine + acetate - ? 380156 2.5.1.47 O3-acetyl-L-serine + methylmercaptan - Geobacillus stearothermophilus S-methyl-L-cysteine + acetate - ? 380157 2.5.1.47 O3-acetyl-L-serine + phenol - Geobacillus stearothermophilus O-phenyl-L-serine + acetate - ? 380158 2.5.1.47 O3-acetyl-L-serine + phenylmercaptan - Geobacillus stearothermophilus S-phenyl-L-cysteine + acetate - ? 380159 2.5.1.47 O3-acetyl-L-serine + propylmercaptan - Geobacillus stearothermophilus S-propyl-L-cysteine + acetate - ? 380160 2.5.1.47 O3-acetyl-L-serine + sulfide - Corynebacterium glutamicum L-cysteine + acetate - ? 380161 2.5.1.47 Ser + sulfide 1.8% of the activity with O-acetyl-L-serine Thermus thermophilus ? - ? 354313