2.5.1.27 (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate + AMP HMBPP, the enzyme uses the hydroxylated side chain precursor to form trans-zeatin-5'-monophosphate in the reaction with AMP, reaction rate is 0.5% of the rate with dimethylallyl díphosphate Nostoc sp. diphosphate + trans-zeatin riboside 5'phosphate - ? 436641 2.5.1.27 (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate + AMP HMBPP, the enzyme uses the hydroxylated side chain precursor to form trans-zeatin-5'-monophosphate in the reaction with AMP, reaction rate is 0.5% of the rate with dimethylallyl díphosphate Nostoc sp. PCC 7120 diphosphate + trans-zeatin riboside 5'phosphate - ? 436641 2.5.1.27 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + AMP - Agrobacterium tumefaciens diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate - ? 374969 2.5.1.27 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + AMP 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is an intermediate in the methylerythritol phosphate pathway. Agrobacterium tumefaciens modifies cytokinin biosynthesis by sending a key enzyme into plastids of the host plant to promote tumorigenesis Agrobacterium tumefaciens diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate - ? 374969 2.5.1.27 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate is a very poor substrate Morus alba diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butenyl)ADP - ? 387460 2.5.1.27 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + AMP - Arabidopsis thaliana diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate - ? 375883 2.5.1.27 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ATP 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate is a very poor substrate Morus alba trans-zeatin riboside 5'-triphosphate + diphosphate - ? 387461 2.5.1.27 dimethylallyl diphosphate + ADP the enzyme prefers ADP and ATP rather than AMP Arabidopsis thaliana ? - ? 376710 2.5.1.27 dimethylallyl diphosphate + ADP the enzyme prefers ADP and ATP rather than AMP Humulus lupulus ? - ? 376710 2.5.1.27 dimethylallyl diphosphate + ADP the enzyme preferrs ADP and ATP rather than AMP Arabidopsis thaliana dimethylallyl-ADP + diphosphate - ? 376711 2.5.1.27 dimethylallyl diphosphate + ADP - Arabidopsis thaliana isopentenyl-ADP + diphosphate - ? 376712 2.5.1.27 dimethylallyl diphosphate + ADP - Morus alba diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate - ir 388560 2.5.1.27 dimethylallyl diphosphate + ADP - Oryza sativa diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate - ? 388560 2.5.1.27 dimethylallyl diphosphate + ADP ADP and ATP are preferred over AMP Zea mays diphosphate + N-(dimethylallyl)adenosine 5'-diphosphate - ? 397042 2.5.1.27 dimethylallyl diphosphate + AMP - Nicotiana tabacum diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP - Glycine max diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP - Arabidopsis thaliana diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP - Agrobacterium tumefaciens diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP - Manihot esculenta diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP - Nostoc sp. diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP - Oryza sativa diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP - Bambusa oldhamii diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP biosynthesis of cytokinins Nicotiana tabacum diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP biosynthesis of cytokinins Arabidopsis thaliana diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP ATP, ADP and cAMP do not function as isopentenyl acceptors Dictyostelium discoideum diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP ATP, ADP and cAMP do not function as isopentenyl acceptors Agrobacterium tumefaciens diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP the enzyme preferrs ADP and ATP rather than AMP Arabidopsis thaliana diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP the enzyme prefers ADP and ATP rather than AMP Arabidopsis thaliana diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP the enzyme prefers ADP and ATP rather than AMP Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP AMP is a poor substrate Morus alba diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ir 323273 2.5.1.27 dimethylallyl diphosphate + AMP - Nostoc sp. PCC 7120 diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate - ? 323273 2.5.1.27 dimethylallyl diphosphate + AMP - Humulus lupulus diphosphate + N-(dimethylallyl)adenosine 5'-phosphate - ir 397043 2.5.1.27 dimethylallyl diphosphate + AMP - Agrobacterium tumefaciens diphosphate + N-(dimethylallyl)adenosine 5'-phosphate - ? 397043 2.5.1.27 dimethylallyl diphosphate + AMP ADP and ATP are preferred over AMP Zea mays diphosphate + N-(dimethylallyl)adenosine 5'-phosphate - ? 397043 2.5.1.27 dimethylallyl diphosphate + ATP the enzyme prefers ADP and ATP rather than AMP Arabidopsis thaliana ? - ? 376713 2.5.1.27 dimethylallyl diphosphate + ATP the enzyme prefers ADP and ATP rather than AMP Humulus lupulus ? - ? 376713 2.5.1.27 dimethylallyl diphosphate + ATP the enzyme preferrs ADP and ATP rather than AMP Arabidopsis thaliana dimethylallyl-ATP + diphosphate - ? 376714 2.5.1.27 dimethylallyl diphosphate + ATP - Morus alba diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate - ir 388562 2.5.1.27 dimethylallyl diphosphate + ATP - Oryza sativa diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate - ? 388562 2.5.1.27 dimethylallyl diphosphate + ATP ADP and ATP are preferred over AMP Zea mays diphosphate + N-(dimethylallyl)adenosine 5'-triphosphate - ? 397044 2.5.1.27 dimethylallyl diphosphate + ATP - Humulus lupulus N6-(dimethylallyl) adenosine 5'-triphosphate + diphosphate - ir 407208 2.5.1.27 dimethylallyl diphosphate + ATP - Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine triphosphate - ? 423345 2.5.1.27 dimethylallyl diphosphate + CDP - Morus alba ? - ir 388563 2.5.1.27 dimethylallyl diphosphate + dADP - Morus alba diphosphate + N6-(dimethylallyl)dADP - ir 388564 2.5.1.27 dimethylallyl diphosphate + dATP - Morus alba diphosphate + N6-(dimethylallyl)dATP - ir 388565 2.5.1.27 dimethylallyl diphosphate + GDP GDP is a poor substrate Morus alba ? - ir 388568 2.5.1.27 dimethylallyl diphosphate + P1,P3-bis(5'-adenosyl)triphosphate - Humulus lupulus ? - ? 423314 2.5.1.27 dimethylallyl diphosphate + P1,P4-bis(5'-adenosyl)tetraphosphate - Humulus lupulus ? - ? 423403 2.5.1.27 dimethylallyl diphosphate + P1,P5-bis(5'-adenosyl)pentaphosphate - Humulus lupulus ? - ? 423404 2.5.1.27 dimethylallyl diphosphate + P1,P6-bis(5'-adenosyl)hexaphosphate - Humulus lupulus ? - ? 423405 2.5.1.27 geranyl diphosphate + ADP geranyl diphosphate is a very poor substrate Morus alba diphosphate + N6-geranyl-ADP - ? 388751 2.5.1.27 isopentenyl diphosphate + ADP isopentyl diphosphate is a very poor substrate Morus alba diphosphate + N6-isopentyl-ADP - ? 388946 2.5.1.27 additional information no substrate: adenine, adenosine, isopentenyldiphosphate Arabidopsis thaliana ? - ? 89 2.5.1.27 additional information strict requirement for AMP, no substrate: adenine, adenosine Nicotiana tabacum ? - ? 89 2.5.1.27 additional information AtIPT3 is upregulated within 1 h after an application of nitrate zo mineral-strved Arabidopsis plants Arabidopsis thaliana ? - ? 89 2.5.1.27 additional information no activity with 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate Arabidopsis thaliana ? - ? 89 2.5.1.27 additional information no activity with GMP, IMP, CMP and UMP Humulus lupulus ? - ? 89 2.5.1.27 additional information farnesyl diphosphate, geranylgeranyl diphosphate, IDP and UDP are no substrates for Morus alba AIPT Morus alba ? - ? 89 2.5.1.27 additional information no substrate: 1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate Zea mays ? - ? 89 2.5.1.27 additional information the plant AIPT predominantly uses ATP or ADP as the isopentenyl acceptor Humulus lupulus ? - ? 89 2.5.1.27 additional information no activity with NAD+, NADP+, and FAD Humulus lupulus ? - ? 89 2.5.1.27 additional information less than 0.5% activity with (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate plus AMP Nostoc sp. ? - ? 89 2.5.1.27 additional information no activity with ADP and ATP. Isopentenyl diphosphate, an isomer of DMAPP, is no substrate with either of the side chain acceptor substrates. The enzyme does not show tRNA IPT activity, as it does not prenylate synthetic tRNA-stem-loop minihelix substrate Nostoc sp. ? - ? 89 2.5.1.27 additional information the enzyme shows no activity with ATP and ADP and no tRNA isopentenyl transferase activity Nostoc sp. ? - ? 89 2.5.1.27 additional information less than 0.5% activity with (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate plus AMP Nostoc sp. PCC 7120 ? - ? 89 2.5.1.27 additional information the enzyme shows no activity with ATP and ADP and no tRNA isopentenyl transferase activity Nostoc sp. PCC 7120 ? - ? 89 2.5.1.27 additional information no activity with ADP and ATP. Isopentenyl diphosphate, an isomer of DMAPP, is no substrate with either of the side chain acceptor substrates. The enzyme does not show tRNA IPT activity, as it does not prenylate synthetic tRNA-stem-loop minihelix substrate Nostoc sp. PCC 7120 ? - ? 89