2.5.1.10 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate - Escherichia coli 5 diphosphate + all-trans-octaprenyl diphosphate - ? 406952 2.5.1.10 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate - Escherichia coli K12 5 diphosphate + all-trans-octaprenyl diphosphate - ? 406952 2.5.1.10 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.29, bifunctional farnesyl/geranylgeranyl diphosphate synthase Plasmodium falciparum diphosphate + geranylgeranyl diphosphate - ? 421728 2.5.1.10 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.29, the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate. Under environmental stresses, Haematococcus pluvialis accumulates large amounts of carotenoids. Scale of carotenoid biosynthesis depends on availability of geranylgeranyl pyrophosphate precursor, which is supplied by GGPP synthase through sequential 1'-4 condensation of three isopentenyl pyrophosphates into dimethylallyl pyrophosphate Haematococcus lacustris diphosphate + geranylgeranyl diphosphate - ? 421728 2.5.1.10 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.29, the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Arabidopsis thaliana diphosphate + geranylgeranyl diphosphate - ? 421728 2.5.1.10 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.29, the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Haematococcus lacustris diphosphate + geranylgeranyl diphosphate - ? 421728 2.5.1.10 (E)-ethynyldimethylallyl diphosphate + isopentenyl diphosphate - Geobacillus stearothermophilus (E)-ethynylfarnesyl diphosphate + diphosphate double condensation product. Yield is 0.8% E-ethynylfarnesyl diphosphate, compared with 100% for the reaction of dimethylallyl diphosphate with isopentenyl diphosphate ? 407625 2.5.1.10 (Z)-ethynyldimethylallyl diphosphate + isopentenyl diphosphate - Sus scrofa (Z)-ethynylfarnesyl diphosphate + diphosphate sole product with wild-type, yield is 21.9% Z-ethynylfarnesyl diphosphate, compared with 100% for the reaction of dimethylallyl diphosphate with isopentenyl diphosphate ? 407687 2.5.1.10 (Z)-ethynyldimethylallyl diphosphate + isopentenyl diphosphate - Geobacillus stearothermophilus (Z)-ethynylfarnesyl diphosphate + diphosphate sole product with wild-type, yield is 88.8% Z-ethynylfarnesyl diphosphate, compared with 100% for the reaction of dimethylallyl diphosphate with isopentenyl diphosphate. Mutant Y81D yields products ethynylgeranyl, ethynylfarnesyl, and ethynylgeranylgeranyl diphosphates ? 407687 2.5.1.10 8-acetoxygeranyl diphosphate + isopentenyl diphosphate - Geobacillus stearothermophilus diphosphate + 12-acetoxyfarnesyl diphosphate 21.8% yield ? 422711 2.5.1.10 8-acetoxygeranyl diphosphate + isopentenyl diphosphate - Geobacillus stearothermophilus ATCC 10149 diphosphate + 12-acetoxyfarnesyl diphosphate 21.8% yield ? 422711 2.5.1.10 8-hydroxygeranyl diphosphate + isopentenyl diphosphate - Geobacillus stearothermophilus hydroxyfarnesyl diphosphate + diphosphate mutants Y81S and Y81D, produce additionally hydroxygeranylgeranyl diphosphate plus a small amount of hydroxygeranylfarnesyl diphosphate ? 396115 2.5.1.10 8-methoxygeranyl diphosphate + isopentenyl diphosphate - Geobacillus stearothermophilus methoxyfarnesyl diphosphate + diphosphate mutants Y81S and Y81D, produce methoxygeranylgeranyl diphosphate as main product plus some methoxyfarnesyl diphosphate ? 396118 2.5.1.10 cyclohexylideneethyl diphosphate + 2-cyclohexenylethyl diphosphate - Geobacillus stearothermophilus (cyclohexylideneethyl)-cyclohexylideneethyl diphosphate relative yield 0.8% ? 409111 2.5.1.10 cyclohexylideneethyl diphosphate + isopentenyl diphosphate - Geobacillus stearothermophilus 10-cyclohexyliden-3,7-dimethyldeca-2,6-dien-1-ol + ? double condensation product, relative yield 23.1% ? 409112 2.5.1.10 cyclohexylideneethyl diphosphate + isopentenyl diphosphate - Sus scrofa 10-cyclohexyliden-3,7-dimethyldeca-2,6-dien-1-ol + ? double condensation product, relative yield 3.0% ? 409112 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate - Bacillus subtilis (E,E)-farnesyl diphosphate + 2 diphosphate - ? 355467 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate - Geobacillus stearothermophilus (E,E)-farnesyl diphosphate + 2 diphosphate - ? 355467 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate - Ricinus communis (E,E)-farnesyl diphosphate + 2 diphosphate - ? 355467 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate - Abies grandis (E,E)-farnesyl diphosphate + 2 diphosphate - ? 355467 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate - Anthonomus grandis (E,E)-farnesyl diphosphate + 2 diphosphate - ? 355467 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate - Panax ginseng (E,E)-farnesyl diphosphate + 2 diphosphate - ? 355467 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate 2 sequential irreversible 1', 4 condensations Homo sapiens (E,E)-farnesyl diphosphate + 2 diphosphate - ir 355467 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate activity is higher than with geranyl diphosphate Pumpkin (E,E)-farnesyl diphosphate + 2 diphosphate - ? 355467 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate no activity with farnesyl diphosphate Geobacillus stearothermophilus (E,E)-farnesyl diphosphate + 2 diphosphate - ? 355467 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate enzyme participates in isoprenoid biosynthesis in eukaryotes Pumpkin (E,E)-farnesyl diphosphate + 2 diphosphate - ? 355467 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate - Streptomyces argenteolus 2 diphosphate + trans,trans-farnesyl diphosphate - ? 376717 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate - Myzus persicae (2E,6E)-farnesyl diphosphate + 2 diphosphate - ? 411996 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate - Rhopalosiphum padi 2 diphosphate + (2E,6E)-farnesyl diphosphate - ? 423323 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate the enzyme is bifunctional and produces (2E,6E)-farnesyl diphosphate or geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate depending on the divalent metal cofactor present. The enzyme is far more active with Co2+ as an additive than with any other tested metal ion. In the presence of Co2+ or Mn2+, with dimethylallyl diphosphate as a cosubstrate, it produces about 96% geranyl diphosphate and only 4% farnesyl diphosphate. In contrast, with Mg2+ as an additive, the enzyme produces 18% geranyl diphosphate and 82% farnesyl diphosphate Phaedon cochleariae 2 diphosphate + (2E,6E)-farnesyl diphosphate - ? 423323 2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate - Chimonanthus praecox 2 diphosphate + farnesyl diphosphate - ? 423392 2.5.1.10 dimethylallyl diphosphate + geranyl diphosphate the enzyme is involved in the biosynthesis of the monoterpenoid precursors needed for formation of the defensive compound chrysomelidial. Farnesyl diphosphate serves as precursor for various primary metabolites and juvenile required hormone Phaedon cochleariae diphosphate + (2E,6E)-farnesyl diphosphate - ? 421953 2.5.1.10 dimethylallyl diphosphate + geranyl diphosphate the production of farnesyl diphosphate from dimethylallyl diphosphate and geranyl diphosphate is most active by addition of 0.5 mM Mg2+ compared with any other tested cofactor. At a constant 0.5 mM Co2+, and an ascending Mg2+ concentration, PcIDS1 displayed low farnesyl diphosphate forming activity. If Mg2+ is constant at 5 mM and Co2+ concentrations vary, high farnesyl diphosphate production can be observed only at Co2+ concentrations below 0.1 mM. As soon as the Co2+ concentration ascends, the farnesyl diphosphate-forming activity decreases dramatically. The Mg2+-catalyzed activity of the enzyme is abolished as soon as Co2+ reaches its optimal concentration Phaedon cochleariae diphosphate + (2E,6E)-farnesyl diphosphate - ? 421953 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate - Streptomyces argenteolus diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate - Sus scrofa diphosphate + geranyl diphosphate relative yield 100% ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate - Geobacillus stearothermophilus diphosphate + geranyl diphosphate relative yield 100% ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate key enzyme in isoprenoid biosynthesis Geobacillus stearothermophilus diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate the synthase amino-acid residues at the 4th and 5th position before the first aspartate rich motif mainly determine product specificity Geobacillus stearothermophilus diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate when (R)-[2-2H]IPP is a substrate for chain elongation, no deuterium is found in the chain elongation products. In contrast, the deuterium in (S)-[2-2H]IPP is incorporated into all of the products. Thus, the pro-R hydrogen at C2 of IPP is lost when the E- and Z-double bond isomers are formed Escherichia coli diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate when (R)-[2-2H]IPP is a substrate for chain elongation, no deuterium is found in the chain elongation products. In contrast, the deuterium in (S)-[2-2H]IPP is incorporated into all of the products. Thus, the pro-R hydrogen at C2 of IPP is lost when the E- and Z-double bond isomers are formed Artemisia tridentata diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.1, the enzyme catalyses the sequential condensation of dimethylallyl diphosphate with isopentenyl diphosphate (IPP) and the resulting geranyl diphosphate (GPP) with another unit of IPP, eventually producing the 15-carbon isoprenoid farnesyl diphosphate Homo sapiens diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.1, the enzyme catalyzes the condensation of dimethylallyl pyrophosphate and isopentenyl pyrophosphate, forming geranyl pyrophosphate and then farnesyl pyrophosphate Homo sapiens diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.1. the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate Saccharomyces cerevisiae diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate. Under environmental stresses, Haematococcus pluvialis accumulates large amounts of carotenoids. Scale of carotenoid biosynthesis depends on availability of geranylgeranyl pyrophosphate precursor, which is supplied by GGPP synthase through sequential 1'-4 condensation of three isopentenyl pyrophosphates into dimethylallyl pyrophosphate Haematococcus lacustris diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate to generate the C15 product (2E,6E)-farnesyl diphosphate, reaction of EC 2.5.1.1 Choristoneura fumiferana diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate to generate the C15 product (2E,6E)-farnesyl diphosphate, reaction of EC 2.5.1.1 Artemisia spiciformis diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of dimethylallyl diphosphate (C5) with isopentenyl diphosphate (IPP) and the resulting geranyl diphosphate (GPP) with another molecule of IPP, eventually producing farnesyl diphosphate which is a precursor for the biosynthesis of a vast majority of isoprenoids Leishmania major diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of dimethylallyl diphosphate with isopentenyl diphosphate and the resulting geranyl diphosphate with another molecule of isopentenyl diphosphate, eventually producing farnesyl diphosphate Leishmania major diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate Saccharomyces cerevisiae diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate the enzyme catalyzes two five-carbon atoms, isopentenyl pyrophosphate (IPP), and its isomer dimethylallyl pyrophosphate phosphoric acid (DMAPP) to form farnesyl diphosphate (FPP) Pinus massoniana diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.1, the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Arabidopsis thaliana diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.1, the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Haematococcus lacustris diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of dimethylallyl diphosphate (C5) with isopentenyl diphosphate (IPP) and the resulting geranyl diphosphate (GPP) with another molecule of IPP, eventually producing farnesyl diphosphate Leishmania major diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.1. the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate Saccharomyces cerevisiae BY4741 diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate Saccharomyces cerevisiae BY4741 diphosphate + geranyl diphosphate - ? 355470 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate - Myzus persicae (E,E)-farnesyl diphosphate + geranyl diphosphate + diphosphate - ? 409312 2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate - Acyrthosiphon pisum (E,E)-farnesyl diphosphate + geranyl diphosphate + diphosphate ratio geranyl diphosphate/farnesyl diphosphate is about 2:1 ? 409312 2.5.1.10 farnesyl diphosphate + isopentenyl diphosphate less than 5% of the activity with dimethylallyl diphosphate Pumpkin geranyl diphosphate + diphosphate - ? 355468 2.5.1.10 geranyl diphosphate + 2-cyclohexenylethyl diphosphate - Geobacillus stearothermophilus (S)-geranylcyclohexylideneethyl diphosphate + diphosphate relative yield 13.6% ? 409481 2.5.1.10 geranyl diphosphate + 2-cyclohexenylethyl diphosphate - Sus scrofa (S)-geranylcyclohexylideneethyl diphosphate + diphosphate relative yield 42.2% ? 409481 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Gallus gallus diphosphate + farnesyl diphosphate - ? 355466 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Homo sapiens diphosphate + farnesyl diphosphate - ir 355466 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Sus scrofa diphosphate + farnesyl diphosphate - ? 355466 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Saccharomyces cerevisiae diphosphate + farnesyl diphosphate - ? 355466 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Geobacillus stearothermophilus diphosphate + farnesyl diphosphate - ? 355466 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Pumpkin diphosphate + farnesyl diphosphate - ? 355466 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Bacillus subtilis diphosphate + farnesyl diphosphate E)-farnesyl diphosphate ? 355466 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Ricinus communis diphosphate + farnesyl diphosphate E)-farnesyl diphosphate ? 355466 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Streptomyces argenteolus diphosphate + trans,trans-farnesyl diphosphate - ? 375134 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate no activity with dimethylallyl diphosphate Mycobacterium tuberculosis diphosphate + trans,trans-farnesyl diphosphate - ? 375134 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate key enzyme in isoprenoid biosynthesis Geobacillus stearothermophilus diphosphate + trans,trans-farnesyl diphosphate - ? 375134 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the synthase amino-acid residues at the 4th and 5th position before the first aspartate rich motif mainly determine product specificity Geobacillus stearothermophilus diphosphate + trans,trans-farnesyl diphosphate - ? 375134 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate small amounts of neryl diphosphate, (Z,E)-farnesyl diphosphate are formed along with the E-isomers. When (R)-[2-2H]IPP is a substrate for chain elongation, no deuterium is found in the chain elongation products. In contrast, the deuterium in (S)-[2-2H]IPP is incorporated into all of the products. Thus, the pro-R hydrogen at C2 of IPP is lost when the E- and Z-double bond isomers are formed Escherichia coli diphosphate + trans,trans-farnesyl diphosphate - ? 375134 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate small amounts of neryl diphosphate, (Z,E)-farnesyl diphosphate are formed along with the E-isomers. When (R)-[2-2H]IPP is a substrate for chain elongation, no deuterium is found in the chain elongation products. In contrast, the deuterium in (S)-[2-2H]IPP is incorporated into all of the products. Thus, the pro-R hydrogen at C2 of IPP is lost when the E- and Z-double bond isomers are formed Artemisia tridentata diphosphate + trans,trans-farnesyl diphosphate - ? 375134 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate no activity with dimethylallyl diphosphate Mycobacterium tuberculosis H37Rv diphosphate + trans,trans-farnesyl diphosphate - ? 375134 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Panax ginseng diphosphate + (E,E)-farnesyl diphosphate - ? 409488 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Homo sapiens diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Zea mays diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Trypanosoma brucei diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Trypanosoma cruzi diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Abies grandis diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Leishmania major diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Toxoplasma gondii diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Geobacillus stearothermophilus diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Actinidia arguta diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Saccharomyces cerevisiae diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Tripterygium wilfordii diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Phaffia rhodozyma diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Pseudomonas aeruginosa diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Penicillium brevicompactum diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Matricaria chamomilla var. recutita diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Rhizosolenia setigera diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate bifunctional farnesyl/geranylgeranyl diphosphate synthase Plasmodium falciparum diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyses the sequential condensation of dimethylallyl diphosphate with isopentenyl diphosphate (IPP) and the resulting geranyl diphosphate (GPP) with another unit of IPP, eventually producing the 15-carbon isoprenoid farnesyl diphosphate Homo sapiens diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate. Under environmental stresses, Haematococcus pluvialis accumulates large amounts of carotenoids. Scale of carotenoid biosynthesis depends on availability of geranylgeranyl pyrophosphate precursor, which is supplied by GGPP synthase through sequential 1'-4 condensation of three isopentenyl pyrophosphates into dimethylallyl pyrophosphate Haematococcus lacustris diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the condensation of dimethylallyl pyrophosphate and isopentenyl pyrophosphate, forming geranyl pyrophosphate and then farnesyl pyrophosphate Homo sapiens diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate to generate the C15 product (2E,6E)-farnesyl diphosphate Choristoneura fumiferana diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate to generate the C15 product (2E,6E)-farnesyl diphosphate Artemisia spiciformis diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of dimethylallyl diphosphate (C5) with isopentenyl diphosphate (IPP) and the resulting geranyl diphosphate (GPP) with another molecule of IPP, eventually producing farnesyl diphosphate which is a precursor for the biosynthesis of a vast majority of isoprenoids Leishmania major diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of dimethylallyl diphosphate with isopentenyl diphosphate and the resulting geranyl diphosphate with another molecule of isopentenyl diphosphate, eventually producing farnesyl diphosphate Leishmania major diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate Saccharomyces cerevisiae diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate Saccharomyces cerevisiae diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes two five-carbon atoms, isopentenyl pyrophosphate (IPP), and its isomer dimethylallyl pyrophosphate phosphoric acid (DMAPP) to form farnesyl diphosphate (FPP) Pinus massoniana diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Arabidopsis thaliana diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Haematococcus lacustris diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of dimethylallyl diphosphate (C5) with isopentenyl diphosphate (IPP) and the resulting geranyl diphosphate (GPP) with another molecule of IPP, eventually producing farnesyl diphosphate Leishmania major diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Toxoplasma gondii RH diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate Saccharomyces cerevisiae BY4741 diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate Saccharomyces cerevisiae BY4741 diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Phaffia rhodozyma ATCC 24230 diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Rhizosolenia setigera CCMP1694 diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Penicillium brevicompactum MUCL 19011 diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 geranyl diphosphate + isopentenyl diphosphate - Geobacillus stearothermophilus ATCC 10149 diphosphate + (2E,6E)-farnesyl diphosphate - ? 423586 2.5.1.10 isopentenyl diphosphate + dimethylallyl diphosphate - Trypanosoma brucei diphosphate + geranyl diphosphate - ? 384943 2.5.1.10 isopentenyl diphosphate + dimethylallyl diphosphate - Homo sapiens diphosphate + geranyl diphosphate - ? 384943 2.5.1.10 isopentenyl diphosphate + dimethylallyl diphosphate - Phaffia rhodozyma diphosphate + geranyl diphosphate - ? 384943 2.5.1.10 isopentenyl diphosphate + dimethylallyl diphosphate - Pseudomonas aeruginosa diphosphate + geranyl diphosphate - ? 384943 2.5.1.10 isopentenyl diphosphate + dimethylallyl diphosphate - Rhizosolenia setigera diphosphate + geranyl diphosphate - ? 384943 2.5.1.10 isopentenyl diphosphate + dimethylallyl diphosphate - Phaffia rhodozyma ATCC 24230 diphosphate + geranyl diphosphate - ? 384943 2.5.1.10 isopentenyl diphosphate + dimethylallyl diphosphate - Rhizosolenia setigera CCMP1694 diphosphate + geranyl diphosphate - ? 384943 2.5.1.10 isopentenyl diphosphate + geranyl diphosphate enzyme participates in isoprenoid biosynthesis in eukaryotes Pumpkin diphosphate + farnesyl diphosphate - ? 370478 2.5.1.10 isopentenyl diphosphate + geranyl diphosphate - Myzus persicae (E,E)-farnesyl diphosphate + diphosphate - ? 409610 2.5.1.10 methoxymethoxydimethylallyl diphosphate + isopentenyl diphosphate - Sus scrofa methoxymethoxygeranyl diphosphate + diphosphate yield is 1% methoxymethoxygeranyl diphosphate, compared with 100% for the reaction of dimethylallyl diphosphate with isopentenyl diphosphate ? 409866 2.5.1.10 methoxymethoxydimethylallyl diphosphate + isopentenyl diphosphate - Geobacillus stearothermophilus methoxymethoxygeranyl diphosphate + diphosphate yield is 5% methoxymethoxygeranyl diphosphate, compared with 100% for the reaction of dimethylallyl diphosphate with isopentenyl diphosphate. Mutant Y81D yields methoxymethoxyfarnesyl diphosphate with high efficiency ? 409866 2.5.1.10 additional information no activity with dimethylallyl diphosphate Mycobacterium tuberculosis ? - ? 89 2.5.1.10 additional information enzyme additionally shows geranyl diphosphate synthase and farnesyl diphosphate synthase activity Myzus persicae ? - ? 89 2.5.1.10 additional information enzyme is able to produce farnesyl phosphate directly from geranyl phosphate, activity of farnesylgeranyltransferase Myzus persicae ? - ? 89 2.5.1.10 additional information FPPS2 has barely detectable geranyl geranyl diphosphate synthase activity Zea mays ? - ? 89 2.5.1.10 additional information isoform FPPS3 with a His-tag at the N-terminus displays a low level of geranyl geranyl diphosphate synthase activity Zea mays ? - ? 89 2.5.1.10 additional information no activity with dimethylallyl diphosphate Mycobacterium tuberculosis H37Rv ? - ? 89 2.5.1.10 propoxygeranyl diphosphate + isopentenyl diphosphate - Geobacillus stearothermophilus propoxyfarnesyl diphosphate + diphosphate yield is 3% methoxymethoxygeranyl diphosphate, compared with 100% for the reaction of dimethylallyl diphosphate with isopentenyl diphosphate, and 63% yield for mutant Y81D, plus 6% of propoxygeranylgeranyl diphosphate ? 410119 2.5.1.10 propoxygeranyl diphosphate + isopentenyl diphosphate - Sus scrofa propoxyfarnesyl diphosphate + diphosphate yield is 62% methoxymethoxygeranyl diphosphate, compared with 100% for the reaction of dimethylallyl diphosphate with isopentenyl diphosphate ? 410119 2.5.1.10 vinyldimethylallyl diphosphate + isopentenyl diphosphate - Geobacillus stearothermophilus vinylfarnesyl diphosphate + diphosphate sole product, yield is 29.9% vinylfarnesyl diphosphate, compared with 100% for the reaction of dimethylallyl diphosphate with isopentenyl diphosphate ? 410635 2.5.1.10 vinyldimethylallyl diphosphate + isopentenyl diphosphate - Sus scrofa vinylgeranyl diphosphate + vinylfarnesyl diphosphate + diphosphate 14.3% vinylgeranyl diphosphate plus 23.3% vinylfarnesyl diphosphate, compared with 100% for the reaction of dimethylallyl diphosphate with isopentenyl diphosphate ? 410636