2.4.2.29	2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one + tRNAguanine	2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one appears to partition between: 1. normal turnover, 2. inactivation, 3. an alternative processing to an unidentrified fluoride-released product	Escherichia coli	guanine + tRNA2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4(3H)-one	-	?	359614	
2.4.2.29	7-(cyano)-7-deazaguanine + tRNAguanine	-	Escherichia coli	guanine + tRNA(7-(cyano)-7-deazaguanine)	inserted 7-(cyano)-7-deazaguanine is located in the first position of the anticodon	?	359606	
2.4.2.29	7-aminomethyl-7-carbaguanine + [RNAECYMA]-2'-deoxyguanine 34	TGT-RNA covalent intermediate	Escherichia coli	guanine + [RNAECYMA]-2'-deoxy-pre Q1	-	?	408227	
2.4.2.29	7-aminomethyl-7-carbaguanine + [RNAECYMA]-guanine	hairpin RNA corresponding to the tRNAtyr anticodon stem-loop motif, TGT-RNA covalent intermediate	Escherichia coli	guanine + [RNAECYMA]-pre Q1	-	?	408228	
2.4.2.29	7-aminomethyl-7-carbaguanine + [tRNA]-guanine	the TGT-RNA covalent complex is kinetically capable of occurring on the TGT reaction pathway. Dissociation of product RNA from the enzyme is overall rate-limiting in the steady state. Role for the 2'-hydroxyl group of the ribose in the TGT reaction	Escherichia coli	guanine + [tRNA]-pre Q1	-	?	408229	
2.4.2.29	7-aminomethyl-7-carbaguanine + [tRNA]-guanine	Tyr106 in the active site is hydrogen bonded in the ligand-free structure	Zymomonas mobilis	guanine + [tRNA]-pre Q1	-	?	408229	
2.4.2.29	guanine + tRNAguanine	-	Escherichia coli	tRNAguanine + guanine	-	r	359610	
2.4.2.29	guanine34 in tRNA + 7-aminomethyl-7-carbaguanine	-	Escherichia coli	7-aminomethyl-7-carbaguanine34 in tRNA + guanine	-	?	359605	
2.4.2.29	guanine34 in tRNA + 7-aminomethyl-7-carbaguanine	-	Plasmodium falciparum	7-aminomethyl-7-carbaguanine34 in tRNA + guanine	-	?	359605	
2.4.2.29	guanine34 in tRNA + 7-aminomethyl-7-carbaguanine	-	Zymomonas mobilis	7-aminomethyl-7-carbaguanine34 in tRNA + guanine	-	?	359605	
2.4.2.29	guanine34 in tRNA + 7-aminomethyl-7-carbaguanine	-	Shigella flexneri	7-aminomethyl-7-carbaguanine34 in tRNA + guanine	-	?	359605	
2.4.2.29	guanine34 in tRNA + 7-aminomethyl-7-carbaguanine	E. coli preQ0-tRNATyr, containing7-(cyano)-7-deazaguanine in the anticodon and E. coli guaninetRNATyr containing guanosine in the anticodon are good acceptors	Escherichia coli	7-aminomethyl-7-carbaguanine34 in tRNA + guanine	-	ir	359605	
2.4.2.29	guanine34 in tRNA + 7-aminomethyl-7-carbaguanine	the enzyme is involved in the hypermodification of cognate tRNAs, leading to the exchange of G34 by 7-methylamino-7-deazaguanine at the wobble position in the anticodon loop	Shigella flexneri	7-aminomethyl-7-carbaguanine34 in tRNA + guanine	-	?	359605	
2.4.2.29	guanine34 in tRNA + 7-aminomethyl-7-carbaguanine	the enzyme catalyzes the posttranscriptional base exchange of the queuosine precursor 7-(aminomethyl)-7-deazaguanine with the genetically encoded guanine in tRNAASp, tRNAAsn, tRNAHis and tRNATyr	Escherichia coli	7-aminomethyl-7-carbaguanine34 in tRNA + guanine	-	?	359605	
2.4.2.29	guanine34 in tRNA + 7-aminomethyl-7-carbaguanine	the enzyme exchanges the genetically encoded guanine at position 34 with a queuine precursor 7-(aminomethyl)-7-deazaguanine	Escherichia coli	7-aminomethyl-7-carbaguanine34 in tRNA + guanine	-	?	359605	
2.4.2.29	guanine34 in tRNA + 7-aminomethyl-7-carbaguanine	the enzyme is responsible for the posttranscriptional modification of specific tRNAs (Asn, Asp, His and Tyr) with queuine. The enzyme catalyzes base exchange of guanosine34 with 7-aminomethyl-7-deazaguanine	Escherichia coli	7-aminomethyl-7-carbaguanine34 in tRNA + guanine	-	?	359605	
2.4.2.29	guanine34 in tRNA + 7-aminomethyl-7-carbaguanine	prefers pre-queuine 1 over pre-queuine 0	Zymomonas mobilis	7-aminomethyl-7-carbaguanine34 in tRNA + guanine	-	?	359605	
2.4.2.29	guanine34 in tRNA + queuine	-	Escherichia coli	queuine34 in tRNA + guanine	-	?	359608	
2.4.2.29	guanine34 in tRNA + queuine	-	Homo sapiens	queuine34 in tRNA + guanine	-	?	359608	
2.4.2.29	additional information	post-translational modification of tRNA	Escherichia coli	?	-	?	89	
2.4.2.29	additional information	higher expression of the subunit TGT60KD in cancer cells than in normal cells. The expression levels of the TGT60KD subunit regulate enzyme activity and the level of queuosine. TGT60KD plays significant roles in carcinogenesis	Homo sapiens	?	-	?	89	
2.4.2.29	additional information	formation of queuosine	Escherichia coli	?	-	?	89	
2.4.2.29	additional information	the 60000 Da subunit of the enzyme plays a role in colon carcinogenesis	Homo sapiens	?	-	?	89	
2.4.2.29	pre-queuine 0 + tRNA guanine	-	Zymomonas mobilis	guanine + tRNA pre-queuine 0	-	?	398140	
2.4.2.29	pre-queuine 1 + virF minihelix RNA	-	Escherichia coli	?	-	?	398141	
2.4.2.29	pre-queuine 1 + virF mRNA	TGT may modulate the translation of VirF via modification of the virF mRNA	Escherichia coli	?	-	?	398142	
2.4.2.29	[tRNATyr]7-aminomethyl-7-carbaguanine + guanine	-	Zymomonas mobilis	[tRNATyr]-guanine + 7-aminomethyl-7-carbaguanine	-	?	410665	
2.4.2.29	[tRNATyr]7-aminomethyl-7-carbaguanine + guanine	TGT active site exhibits a hydrophobic patch formed by Val45 and Leu68, where the phosphate, linking ribose-34 to ribose-35, in bound tRNA is located	Zymomonas mobilis	[tRNATyr]-guanine + 7-aminomethyl-7-carbaguanine	-	?	410665	
2.4.2.29	[tRNATyr]7-aminomethyl-7-carbaguanine + guanine	Tgt dimer binds specifically to a single tRNA molecule	Zymomonas mobilis	[tRNATyr]-guanine + 7-aminomethyl-7-carbaguanine	-	?	410665