2.4.1.B72	Ac-WAEW-NH2 peptide + GDP-mannose	-	Caenorhabditis elegans	?	-	?	457732	
2.4.1.B72	Ac-WAEW-NH2 peptide + GDP-mannose	-	Plasmodium falciparum	?	-	?	457732	
2.4.1.B72	Ac-WAEW-NH2 peptide + GDP-mannose	-	Toxoplasma gondii	?	-	?	457732	
2.4.1.B72	Ac-WAEW-NH2 peptide + GDP-mannose	-	Toxoplasma gondii VEG	?	-	?	457732	
2.4.1.B72	Ac-WAEW-NH2 peptide + GDP-mannose	-	Toxoplasma gondii ATCC 50861	?	-	?	457732	
2.4.1.B72	Ac-WAEWGEC-NH2 peptide + GDP-mannose	-	Caenorhabditis elegans	?	-	?	457733	
2.4.1.B72	Ac-WAEWGEC-NH2 peptide + GDP-mannose	-	Plasmodium falciparum	?	-	?	457733	
2.4.1.B72	Ac-WAEWGEC-NH2 peptide + GDP-mannose	-	Toxoplasma gondii	?	-	?	457733	
2.4.1.B72	Ac-WAEWGEC-NH2 peptide + GDP-mannose	-	Toxoplasma gondii VEG	?	-	?	457733	
2.4.1.B72	Ac-WAEWGEC-NH2 peptide + GDP-mannose	-	Toxoplasma gondii ATCC 50861	?	-	?	457733	
2.4.1.B72	dolichyl D-mannosyl phosphate + ADAMTS-like 1	purified recombinant tagged substrate. The substrate contains in thrombospondin type-1 repeats, two with predicted C-mannosylation sites, C-mannosylation of TSR1	Cricetulus griseus	dolichyl phosphate + C-mannosylated ADAMTS-like 1	-	?	435466	
2.4.1.B72	dolichyl D-mannosyl phosphate + human punctin-1	purified recombinant tagged substrate. C-mannosylation at Trp39 and Trp42 of a thrombospondin type-1 repeat containing the 36WDAWGPWSECSRTC49 sequence, identified by Tandem mass spectrometry (MS/MS) and MS/MS/MS analysis, TSR1 from punctin-1 carries C-mannosylation in close proximity to O-linked fucose	Cricetulus griseus	dolichyl phosphate + C-mannosylated human punctin-1	-	?	435467	
2.4.1.B72	dolichyl D-mannosyl phosphate + MIG-21 protein	C-mannosylation at a tryptophan residue	Caenorhabditis elegans	dolichyl phosphate + C-mannosylated MIG-21 protein	-	?	434653	
2.4.1.B72	dolichyl D-mannosyl phosphate + UNC-5 protein	C-mannosylation at a tryptophan residue	Caenorhabditis elegans	dolichyl phosphate + C-mannosylated UNC-5 protein	-	?	434654	
2.4.1.B72	dolichyl D-mannosyl phosphate + WAKW	in vitro assay using radiolabeled donor substrate and a synthetic acceptor peptide WAKW, which forms the minimal acceptor substrate	Caenorhabditis elegans	dolichyl phosphate + ?	-	?	435468	
2.4.1.B72	additional information	cell surface receptors MIG-21 and UNC-5 are acceptor substrates of enzyme DPY-19	Caenorhabditis elegans	?	-	?	89	
2.4.1.B72	additional information	DPY19L3 is the C-mannosyltransferase of Rspo1 at residue W156	Homo sapiens	?	-	?	89	
2.4.1.B72	additional information	protein C-mannosylation is the attachment of alpha-mannopyranose to tryptophan via a C-C linkage. This post-translational modification typically occurs within the sequence motif WXXW, which is frequently present in thrombospondin type-1 repeats (TSRs). TSRs are especially numerous in and a defining feature of the ADAMTS superfamily. Predicted C-mannosylation sites in the ADAMTS superfamily, overview	Cricetulus griseus	?	-	?	89	
2.4.1.B72	additional information	analysis of C-mannosylation sites an substrate recognition, mutational analysis, mass spectroscopy, and metabolic labeling, overview	Cricetulus griseus	?	-	?	89	
2.4.1.B72	additional information	C-mannosylation of protein Rspo1, Rspo1 has two predicted C-mannosylation sites in the thrombospondin type 1 repeat TSR1, substrate structure, overview. Enzyme DPY19L3 selectively modified Rspo1 at residue W156 but not W153 based on mass spectrometry. Purified recombinant Rspo1 protein from the conditioned medium of overexpressing HT1080-Rspo1-MH cells	Homo sapiens	?	-	?	89	
2.4.1.B72	additional information	C-mannosylation of tryptophan residues is an endoplasmic reticulum localized, DPY-19 enzyme-catalyzed reaction using the lipid-linked glycoside dolichol-phosphate-mannose as the donor substrate	Caenorhabditis elegans	?	-	?	89	
2.4.1.B72	additional information	C-mannosylation is initially described as a modification of the first tryptophan in the WxxW consensus sequence, but is also observed on tryptophans that are not part of this motif. In addition to WxxW, WxxC is also considered to be a consensus sequence for C-mannosylation	Caenorhabditis elegans	?	-	-	89	
2.4.1.B72	additional information	mammalian C-mannosyltransferases DPY19L1 and DPY19L3 do not have a dual WxxW/C recognition motif, but are active C-mannosyltransferases with distinct substrate specificities	Mus musculus	?	-	-	89	
2.4.1.B72	additional information	the enzyme can transfer mannose to adhesive thrombospondin type 1 repeats (TSRs) and type I cytokine receptors. Enzyme Dpy19 of Caenorhabditis elegans can transfer mannose to a short WXXW peptide	Caenorhabditis elegans	?	-	-	89	
2.4.1.B72	additional information	the micronemal adhesin thrombospondin-related anonymous protein (TRAP) is C-hexosylated in Plasmodium falciparum sporozoites. When expressed in a mammalian cell line deficient in C-mannosylation, the Plasmodium falciparum Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide	Plasmodium falciparum	?	-	-	89	
2.4.1.B72	additional information	the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated	Toxoplasma gondii	?	-	-	89	
2.4.1.B72	additional information	no activity by DPY19L4 isozyme on murine UNC5A	Mus musculus	?	-	-	89	
2.4.1.B72	additional information	several thrombospondin type 1 repeat (TSR) domain-containing proteins of Plasmodium falciparum can be C-mannosylated in vivo	Plasmodium falciparum	?	-	-	89	
2.4.1.B72	additional information	the single Caenorhabditis elegans C-mannosyltransferase DPY-19 mannosylates the first two tryptophans of a WxxWxxW motif	Caenorhabditis elegans	?	-	-	89	
2.4.1.B72	additional information	the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated	Toxoplasma gondii VEG	?	-	-	89	
2.4.1.B72	additional information	the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated	Toxoplasma gondii ATCC 50861	?	-	-	89	
2.4.1.B72	UNC5A + GDP-mannose	C-mannosylation of murine UNC5A	Caenorhabditis elegans	C-mannosyl-UNC5A + GDP	-	?	458349	
2.4.1.B72	UNC5A + GDP-mannose	C-mannosylation of murine UNC5A, DPY19L1 can C-mannosylate the first two tryptophans in the WxxWxxWxxC sequence of UNC5A TSRs	Mus musculus	C-mannosyl-UNC5A + GDP	-	?	458349	
2.4.1.B72	UNC5A + GDP-mannose	C-mannosylation of murine UNC5A, DPY19L3 mannosylates the third tryptophan of this sequence and might require a WxxC motif	Mus musculus	C-mannosyl-UNC5A + GDP	-	?	458349