2.4.1.85 additional information final enzyme of dhurrin biosynthesis Sorghum bicolor ? - ? 89 2.4.1.85 additional information UGT85B1 has a broad substrate specificity Sorghum bicolor ? - ? 89 2.4.1.85 additional information reaction is specific for an aromatic group, but not for a p-hydroxy substituent on the aromatic group, no substrates: catechol, resorcinol, phloroglucinol, fructose, glucose, dhurrin, acetone cyanohydrin, acetaldehyde cyanohydrin, GDPglucose, TDPglucose, ADPglucose Sorghum bicolor additional information no product: taxiphyllin ? 1440 2.4.1.85 additional information exclusively specific for the presence of a benzyl group in the substrate, no substrate: acetone cyanohydrin, hydroquinone, p-hydroxybenzaldehyde, gentisic acid, caffeic acid, 2-hydroxy cinnamic acid, resveratrol, salicylic acid, p-hydroxymandelic acid, quercetin, caynidin, biochanin A, naringenin, apigenin, indole acetic acid, tomatidine Sorghum bicolor additional information - ? 1440 2.4.1.85 UDP-alpha-D-glucose + phenol very poor substrate Sorghum bicolor UDP + phenyl beta-D-glucopyranoside - ? 323018 2.4.1.85 UDP-D-glucose + (S)-4-hydroxymandelonitrile - Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside i.e. dhurrin ? 434840 2.4.1.85 UDP-D-glucose + (S)-4-hydroxymandelonitrile the glucosylation stabilizes the labile cyanohydrin Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside i.e. dhurrin ? 434840 2.4.1.85 UDP-glucose + (R,S)-mandelonitrile - Prunus dulcis UDP + prunasin - ir 407426 2.4.1.85 UDP-glucose + 1-hexanol - Sorghum bicolor UDP + hexyl beta-D-glucoside - ? 377982 2.4.1.85 UDP-glucose + 2-hydroxy-2-methylbutyronitrile - Sorghum bicolor UDP + ? - ? 377989 2.4.1.85 UDP-glucose + 2-hydroxy-3-methoxybenzyl alcohol - Sorghum bicolor UDP + ? - ? 377990 2.4.1.85 UDP-glucose + 3-methyl-2-buten-1-ol - Sorghum bicolor UDP + 3-methyl-2-butenyl-beta-D-glucoside - ? 377993 2.4.1.85 UDP-glucose + 3-methyl-3-buten-1-ol - Sorghum bicolor UDP + 3-methyl-3-butenyl-beta-D-glucoside - ? 377994 2.4.1.85 UDP-glucose + 4-hydroxybenzaldehyde poor substrate, mixture of 4-hydroxybenzaldehyde with NaCN: good substrate Sorghum bicolor UDP + 4-formylphenyl beta-D-glucopyranoside - ? 259626 2.4.1.85 UDP-glucose + 4-hydroxymandelonitrile - Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside - ? 377998 2.4.1.85 UDP-glucose + acetone cyanohydrin - Sorghum bicolor UDP + ? - ? 378005 2.4.1.85 UDP-glucose + alpha-terpinol - Sorghum bicolor UDP + alpha-terpinyl-beta-D-glucoside - ? 378006 2.4.1.85 UDP-glucose + benzyl alcohol - Prunus dulcis UDP + benzyl beta-D-glucoside - ? 410561 2.4.1.85 UDP-glucose + beta-citronellol - Sorghum bicolor UDP + beta-citronellol beta-D-glucoside - ? 378008 2.4.1.85 UDP-glucose + cis-3-hexen-1-ol - Sorghum bicolor UDP + cis-3-hexenyl-beta-D-glucoside - ? 378015 2.4.1.85 UDP-glucose + farnesol - Sorghum bicolor UDP + farnesyl beta-D-glucoside - ? 378025 2.4.1.85 UDP-glucose + geraniol - Sorghum bicolor UDP + geranyl-beta-D-glucoside - ? 378028 2.4.1.85 UDP-glucose + linalool - Sorghum bicolor UDP + linalool beta-D-glucoside - ? 378036 2.4.1.85 UDP-glucose + mandelonitrile - Sorghum bicolor UDP + 2-(beta-D-glucopyranosyl)-2-phenylacetonitrile - ? 378042 2.4.1.85 UDP-glucose + mandelonitrile specific for UDP-glucose Sorghum bicolor UDP + mandelonitrile beta-D-glucoside - ? 378043 2.4.1.85 UDP-glucose + nerol - Sorghum bicolor UDP + nerol beta-D-glucoside - ? 378046 2.4.1.85 UDP-glucose + trans-2-hexen-1-ol - Sorghum bicolor UDP + trans-2-hexenyl-beta-D-glucoside - ? 378060 2.4.1.85 UDP-glucose + vanillic acid - Sorghum bicolor UDP + vanillic acid beta-D-glucoside - ? 378063 2.4.1.85 UDP-glucose + vanillin - Sorghum bicolor UDP + vanillin beta-D-glucoside - ? 378064 2.4.1.85 UDPalpha-D-glucose + benzoic acid at 4% of the rate with p-hydroxymandelonitrile Sorghum bicolor UDP + 1-O-benzoyl-beta-D-glucose - ? 323020 2.4.1.85 UDPglucose + (S)-4-hydroxymandelonitrile - Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside identical with dhurrin ? 323012 2.4.1.85 UDPglucose + (S)-4-hydroxymandelonitrile C-terminal region encodes the UDPglucose binding domain Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside identical with dhurrin ? 323012 2.4.1.85 UDPglucose + (S)-4-hydroxymandelonitrile stereospecific for the S-enantiomer Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside identical with dhurrin ? 323012 2.4.1.85 UDPglucose + (S)-4-hydroxymandelonitrile involved in dhurrin biosynthesis Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside dhurrin is the cyanogenic glucoside characteristic of Sorghum species ? 323012 2.4.1.85 UDPglucose + (S)-4-hydroxymandelonitrile endogenous substrate is p-hydroxymandelonitrile, final step in the biosynthesis of dhurrin, transformation of the labile cyanohydrin into a stable storage form Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside - ? 323012 2.4.1.85 UDPglucose + (S)-mandelonitrile at 78% of the rate with p-hydroxymandelonitrile Sorghum bicolor UDP + (S)-mandelonitrile beta-D-glucoside - ? 323013 2.4.1.85 UDPglucose + (S)-mandelonitrile as good as 4-hydroxymandelonitrile Sorghum bicolor UDP + (S)-mandelonitrile beta-D-glucoside - ? 323013 2.4.1.85 UDPglucose + 4-hydroxybenzoic acid poor substrate Sorghum bicolor UDP + glucosyl-4-hydroxybenzoate - ? 323017 2.4.1.85 UDPglucose + 4-hydroxybenzyl alcohol at 36% the rate of 4-hydroxymandelonitrile Sorghum bicolor UDP + 4-hydroxybenzyl glucoside - ? 323015 2.4.1.85 UDPglucose + benzyl alcohol at 13% of the rate with p-hydroxymandelonitrile Sorghum bicolor UDP + benzylglucoside - ? 323019 2.4.1.85 UDPglucose + geraniol at 11% of the rate with p-hydroxymandelonitrile Sorghum bicolor UDP + geraniol glucoside - ? 323021 2.4.1.85 UDPglucose + hydroquinone at 41% the rate of 4-hydroxymandelonitrile Sorghum bicolor UDP + hydroquinone glucoside - ? 323014