2.4.1.288 dTDP-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl - Mycobacterium tuberculosis UDP + ? - ? 420639 2.4.1.288 GDP-alpha-D-glucopyranose + methyl alpha-D-mannopyrannoside - Leishmania major UDP + ? - ? 457983 2.4.1.288 GDP-alpha-D-mannopyranose + methyl alpha-D-mannopyrannoside - Leishmania major UDP + ? - ? 457984 2.4.1.288 additional information GlfT2 has one active site pocket capable of catalyzing both beta-(1->5) and beta-(1->6) galactofuranosyl transfer reactions Mycobacterium tuberculosis ? - ? 89 2.4.1.288 additional information GlfT2 mediates synthesis of the galactan polymer, which contains between 20 and 40 D-galactofuranose residues connected by alternating beta-D-galactofuranosyl-(1->5)-D-galactofuranose and beta-D-galactofuranosyl-(1->6)-D-galactofuranose linkages. Elongation occurs by addition of D-galactofuranose residues from UDP-alpha-D-galactofuranose to the nonreducing end of a lipid-linked acceptor Mycobacterium tuberculosis ? - ? 89 2.4.1.288 additional information no activity with 4-phenoxy-but-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside Mycobacterium tuberculosis ? - ? 89 2.4.1.288 additional information octyl alpha-L-rhamnopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-glucopyranoside and octyl alpha-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-glucopyranoside are no effective substrates Mycolicibacterium smegmatis ? - ? 89 2.4.1.288 additional information GlfT2 is promiscuous in its substrate preferences. It preferentially acts on substrates in which the lipid-bearing Gal residue is connected to the sequence by a (1->)-beta-glycosidic linkage. The preferential formation of alternating (1->6)-beta-Galf and (1->5)-beta-Galf residues arises not from substrate binding but during catalytic processive polymerization Mycobacterium tuberculosis ? - - 89 2.4.1.288 additional information among hexoses, monosaccharides, 4-nitrophenyl-furanoses, 4-nitorphenyl-hexoses and disaccharides tested, only methyl alpha-D-mannopyrannoside efficiently reacts as an acceptor. No donor: UDP-alpha-D-glucuronic acid Leishmania major ? - - 89 2.4.1.288 additional information among hexoses, monosaccharides, 4-nitrophenyl-furanoses, 4-nitorphenyl-hexoses and disaccharides tested, only methyl alpha-D-mannopyrannoside efficiently reacts as an acceptor. No donors: UDP-alpha-D-glucopyranose, UDP-alpha-D-glucuronic acid, GDP-alpha-D-glucopyranose, GDP-alpha-D-mannopyranose Leishmania major ? - - 89 2.4.1.288 additional information GlfT2 is promiscuous in its substrate preferences. It preferentially acts on substrates in which the lipid-bearing Gal residue is connected to the sequence by a (1->6)-beta-glycosidic linkage. The preferential formation of alternating (1->6)-beta-Galf and (1->5)-beta-Galf residues arises not from substrate binding but during catalytic processive polymerization Mycobacterium tuberculosis ? - - 89 2.4.1.288 additional information GlfT2 mediates synthesis of the galactan polymer, which contains between 20 and 40 D-galactofuranose residues connected by alternating beta-D-galactofuranosyl-(1->5)-D-galactofuranose and beta-D-galactofuranosyl-(1->6)-D-galactofuranose linkages. Elongation occurs by addition of D-galactofuranose residues from UDP-alpha-D-galactofuranose to the nonreducing end of a lipid-linked acceptor Mycobacterium tuberculosis H37Rv ? - ? 89 2.4.1.288 additional information no activity with 4-phenoxy-but-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside Mycobacterium tuberculosis H37Rv ? - ? 89 2.4.1.288 additional information GlfT2 is promiscuous in its substrate preferences. It preferentially acts on substrates in which the lipid-bearing Gal residue is connected to the sequence by a (1->)-beta-glycosidic linkage. The preferential formation of alternating (1->6)-beta-Galf and (1->5)-beta-Galf residues arises not from substrate binding but during catalytic processive polymerization Mycobacterium tuberculosis ATCC 25618 ? - - 89 2.4.1.288 additional information GlfT2 is promiscuous in its substrate preferences. It preferentially acts on substrates in which the lipid-bearing Gal residue is connected to the sequence by a (1->6)-beta-glycosidic linkage. The preferential formation of alternating (1->6)-beta-Galf and (1->5)-beta-Galf residues arises not from substrate binding but during catalytic processive polymerization Mycobacterium tuberculosis ATCC 25618 ? - - 89 2.4.1.288 UDP-2''-O-methyl-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl - Mycobacterium tuberculosis UDP + ? - ? 421596 2.4.1.288 UDP-3''-deoxy-alpha-D-xylo-hexofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl - Mycobacterium tuberculosis UDP + ? - ? 421600 2.4.1.288 UDP-3-deoxy-alpha-D-threo-hexofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl - Mycobacterium tuberculosis UDP + ? - ? 421602 2.4.1.288 UDP-5''-O-methyl-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl - Mycobacterium tuberculosis UDP + ? - ? 421603 2.4.1.288 UDP-5-deoxy-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl - Mycobacterium tuberculosis UDP + ? - ? 421604 2.4.1.288 UDP-5-deoxy-alpha-D-galactofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl - Mycobacterium tuberculosis UDP + ? - ? 421605 2.4.1.288 UDP-6''-O-methyl-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl - Mycobacterium tuberculosis UDP + ? - ? 421606 2.4.1.288 UDP-6-deoxy-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl - Mycobacterium tuberculosis UDP + ? - ? 421607 2.4.1.288 UDP-6-deoxy-alpha-D-galactofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl - Mycobacterium tuberculosis UDP + ? - ? 421608 2.4.1.288 UDP-alpha-D-arabinofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl - Mycobacterium tuberculosis UDP + ? - ? 421609 2.4.1.288 UDP-alpha-D-arabinofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl - Mycobacterium tuberculosis UDP + ? - ? 421610 2.4.1.288 UDP-alpha-D-arabinofuranose + octyl beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf - Mycobacterium tuberculosis ? - ? 458250 2.4.1.288 UDP-alpha-D-arabinofuranose + octyl beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf - Mycobacterium tuberculosis ATCC 25618 ? - ? 458250 2.4.1.288 UDP-alpha-D-galactofuranose + 12-phenoxy-dodec-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside the enzyme functions as a processive polymerase only with 12-phenoxy-dodec-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside Mycobacterium tuberculosis UDP + ? - ? 421611 2.4.1.288 UDP-alpha-D-galactofuranose + 12-phenoxy-dodec-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside the enzyme functions as a processive polymerase only with 12-phenoxy-dodec-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside Mycobacterium tuberculosis H37Rv UDP + ? - ? 421611 2.4.1.288 UDP-alpha-D-galactofuranose + 19-phenoxy-nondec-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside - Mycobacterium tuberculosis UDP + ? - ? 421612 2.4.1.288 UDP-alpha-D-galactofuranose + 19-phenoxy-nondec-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside - Mycobacterium tuberculosis H37Rv UDP + ? - ? 421612 2.4.1.288 UDP-alpha-D-galactofuranose + 9-phenoxy-non-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside - Mycobacterium tuberculosis UDP + ? - ? 421613 2.4.1.288 UDP-alpha-D-galactofuranose + 9-phenoxy-non-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside - Mycobacterium tuberculosis H37Rv UDP + ? - ? 421613 2.4.1.288 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol - Mycolicibacterium smegmatis UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol - ? 421615 2.4.1.288 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol - Mycobacterium tuberculosis UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol - ? 421615 2.4.1.288 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol the beta-(1->5)-transferase activity of the enzyme first glycosylates the galactofuranosyl residue at the nonreducing terminus of the polyprenol-bound tetrasaccharide acceptor. The resulting product is then a substrate for the beta-(1->6)-transferase activity of the enzyme Mycobacterium tuberculosis UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol - ? 421615 2.4.1.288 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol the enzyme adds the third and subsequent alpha-D-galactofuranosyl residues to the growing galactan chain Mycobacterium tuberculosis UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol - ? 421615 2.4.1.288 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol - Mycobacterium tuberculosis H37Rv UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol - ? 421615 2.4.1.288 UDP-alpha-D-galactofuranose + beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-O(CH2)7CH3 - Mycobacterium tuberculosis beta-D-Galf-(1->6)-beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-O(CH2)7CH3 - ? 421616 2.4.1.288 UDP-alpha-D-galactofuranose + beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-O(CH2)7CH3 - Mycobacterium tuberculosis UDP + ? - ? 421617 2.4.1.288 UDP-alpha-D-galactofuranose + beta-D-Galf-(1->6)-beta-D-Galf-(1->5)-beta-D-Galf-O(CH2)7CH3 - Mycobacterium tuberculosis ? - ? 421618 2.4.1.288 UDP-alpha-D-galactofuranose + beta-D-Galf-(1->6)-beta-D-Galf-(1->5)-beta-D-Galf-O(CH2)7CH3 - Mycobacterium tuberculosis UDP + beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-(1->5)-beta-D-Galf-O(CH2)7CH3 - ? 421619 2.4.1.288 UDP-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl best donor-acceptor combination, dual recognition of both the terminal C-5 and C-6 hydroxyl groups of the acceptor substrate are required for GlfT2 activity Mycobacterium tuberculosis UDP + ? - ? 421620 2.4.1.288 UDP-alpha-D-galactofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl - Mycobacterium tuberculosis UDP + ? - ? 421621 2.4.1.288 UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose nonnatural substrate Mycobacterium tuberculosis UDP + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose - ? 458251 2.4.1.288 UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose - Mycobacterium tuberculosis UDP + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose - ? 457272 2.4.1.288 UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose natural substrate Mycobacterium tuberculosis UDP + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose - ? 457272 2.4.1.288 UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose - Mycobacterium tuberculosis ATCC 25618 UDP + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose - ? 457272 2.4.1.288 UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose natural substrate Mycobacterium tuberculosis ATCC 25618 UDP + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose - ? 457272 2.4.1.288 UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranose - Mycobacterium tuberculosis UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose - ? 457273 2.4.1.288 UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranose - Mycobacterium tuberculosis ATCC 25618 UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose - ? 457273 2.4.1.288 UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose - Mycobacterium tuberculosis UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose - ? 457274 2.4.1.288 UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose natural substrate Mycobacterium tuberculosis UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranose - ? 457274 2.4.1.288 UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose natural substrate Mycobacterium tuberculosis UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose - ? 457275 2.4.1.288 UDP-alpha-D-galactofuranose + D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose nonnatural substrate Mycobacterium tuberculosis UDP + D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranose - ? 458252 2.4.1.288 UDP-alpha-D-galactofuranose + dec-2-enyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranoside - Mycobacterium tuberculosis UDP + ? - ? 421622 2.4.1.288 UDP-alpha-D-galactofuranose + methyl alpha-D-mannopyrannoside - Leishmania major UDP + ? - ? 458253 2.4.1.288 UDP-alpha-D-galactofuranose + octyl beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-glucopyranoside - Mycolicibacterium smegmatis UDP + octyl beta-D-galactofuranosyl-(1->6)-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-glucopyranoside - ? 421623 2.4.1.288 UDP-alpha-D-galactopyranose + methyl alpha-D-mannopyrannoside - Leishmania major UDP + ? - ? 458254 2.4.1.288 UDP-alpha-D-glucopyranose + methyl alpha-D-mannopyrannoside - Leishmania major UDP + ? - ? 458275