2.4.1.27	additional information	the AGT with GT-B glycosyltransferase fold is responsible for DNA base flipping and substrate recognition, mechanism, overview	Tequatrovirus T4	?	-	?	89	
2.4.1.27	additional information	the alpha-linkage forming enzyme AGT is also able to adopt the fold of a GT-B glycosyltransferase keeping the sugar donor binding mechanism typical for GT-B glycosyltransferases, but showing a unique sugar acceptor binding mode, both substrates are bound to the C-terminal domain, detailed substrate binding structures, catalytic mechanism of both enzyme types, overview	Tequatrovirus T4	?	-	?	89	
2.4.1.27	additional information	enzyme shows strong preference for the glucosylation of DNA containing symmetrical 5-hydroxymethyl cytosine resiudes over hemi-5-hydroxymethyl cytosine. The enzyme is capable of saturation glucosylation on all conformations of 5-hydroxymethyl cytosine.The numbers of 5-hydroxymethyl cytosine residues on DNA can influence catalysis, especially the rate of catalysis, and hemihydroxymethylated DNA is not the preferred substrate of the enzyme	Tequatrovirus T4	?	-	?	89	
2.4.1.27	UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA	-	Tequatrovirus T4	UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA	-	-	382989	
2.4.1.27	UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA	-	Tequatrovirus T4	UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA	-	?	382989	
2.4.1.27	UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA	DNA-modifying enzyme	Tequatrovirus T4	UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA	-	?	382989	
2.4.1.27	UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA	the enzyme forms part of a phage DNA protection system	Tequatrovirus T4	UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA	-	?	382989	
2.4.1.27	UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA	the enzyme is part of a system by which T-even bacteriophages glycosylate their genomic DNA in order to protect it against nuclease attack	Tequatrovirus T4	UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA	-	?	382989	
2.4.1.27	UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA	the enzyme modifies T4-DNA	Tequatrovirus T4	UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA	-	?	382989	
2.4.1.27	UDP-alpha-D-glucose + 5-hydroxymethylcytosine-containing DNA	-	Tequatrovirus T4	UDP + beta-D-glucosyl-5-hydroxymethylcytosine-containing DNA	-	-	454668	
2.4.1.27	UDP-D-glucose + 5-hydroxymethyl cytosine	5-hydroxymethyl cytosine residues of duplex DNA	Tequatrovirus T4	UDP + 5-hydroxymethyl cytosine beta-D-glucoside	-	?	375343	
2.4.1.27	UDP-D-glucose + 5-hydroxymethyl cytosine containing DNA	-	Tequatrovirus T4	UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing DNA	recombinant enzyme glucosylates 5-hydroxymethyl cytosine containing DNA in a nonprocessive manner, and binding to either 5-hydroxymethyl cytosine containing DNA or uridine diphosphoglucose substrates is random, with both binary complexes being catalytically competent. The numbers of 5-hydroxymethyl cytosine on target sequences influence the turnover numbers for the recombinant enzyme	?	421638	
2.4.1.27	UDP-D-glucose + 5-hydroxymethyl cytosine containing phage T4 DNA	-	Tequatrovirus T4	UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing phage T4 DNA	-	?	421639	
2.4.1.27	UDP-D-glucose + DNA containing 12 5-hydroxymethyl cytosine residues	-	Tequatrovirus T4	UDP + ?	-	?	421640	
2.4.1.27	UDP-D-glucose + DNA containing 2 5-hydroxymethyl cytosine residues	-	Tequatrovirus T4	UDP + ?	-	?	421641	
2.4.1.27	UDP-D-glucose + DNA containing 24 5-hydroxymethyl cytosine residues	-	Tequatrovirus T4	UDP + ?	-	?	421642	
2.4.1.27	UDP-D-glucose + DNA containing 6 5-hydroxymethyl cytosine residues	-	Tequatrovirus T4	UDP + ?	-	?	421643