2.4.1.140 2 sucrose - Leuconostoc citreum ? - ? 457457 2.4.1.140 2 sucrose - Leuconostoc citreum NRRL B-1355 ? - ? 457457 2.4.1.140 2 sucrose - Leuconostoc citreum ABK-1 ? - ? 457457 2.4.1.140 additional information methyl D-allopyrano-sides are glucosylated primarily at position 6, yielding methyl alpha-D-glucopyranosyl-(1,6)-D-allopyranosides. The latter subsequently gave rise to methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-D-allopyranosides. The methyl alpha-D-hexopyranosides are better acceptors than the corresponding beta-glycosides Leuconostoc mesenteroides ? - ? 89 2.4.1.140 additional information LC/MS product analysis Leuconostoc citreum ? - ? 89 2.4.1.140 additional information alternansucrase enzyme from Leuconostoc citreum SK24.002 is used to produce di-glycosyl-stevioside through acceptor reaction. Identification of the di-glycosyl-stevioside structure as 13-([alpha-D-glucopyranosyl-(1->3)-alpha-D-glucopyranosyl-(1->6)-beta-D glucopyranosyl-(1->2)-beta-D-glucopyranosyl]oxy)kaur-16-en-19-oic acid beta-D glucopyranosyl ester, method optimization and evaluation, overview Leuconostoc citreum ? - - 89 2.4.1.140 additional information for the docking calculations, a model of the ASRDELTA2 glucosyl-enzyme intermediate is constructed based on the high resolution structure of the GH13 covalent intermediate (PDB ID 1S46). Maltose is the best known acceptor for ASR Leuconostoc citreum ? - - 89 2.4.1.140 additional information product analysis, determination of alternan nanoparticle size distribution by dynamic light scattering Leuconostoc citreum ? - - 89 2.4.1.140 additional information LC/MS product analysis Leuconostoc citreum SK24.002 ? - ? 89 2.4.1.140 additional information alternansucrase enzyme from Leuconostoc citreum SK24.002 is used to produce di-glycosyl-stevioside through acceptor reaction. Identification of the di-glycosyl-stevioside structure as 13-([alpha-D-glucopyranosyl-(1->3)-alpha-D-glucopyranosyl-(1->6)-beta-D glucopyranosyl-(1->2)-beta-D-glucopyranosyl]oxy)kaur-16-en-19-oic acid beta-D glucopyranosyl ester, method optimization and evaluation, overview Leuconostoc citreum SK24.002 ? - - 89 2.4.1.140 additional information for the docking calculations, a model of the ASRDELTA2 glucosyl-enzyme intermediate is constructed based on the high resolution structure of the GH13 covalent intermediate (PDB ID 1S46). Maltose is the best known acceptor for ASR Leuconostoc citreum NRRL B-1355 ? - - 89 2.4.1.140 additional information product analysis, determination of alternan nanoparticle size distribution by dynamic light scattering Leuconostoc citreum ABK-1 ? - - 89 2.4.1.140 stevioside + sucrose transglucosylation Leuconostoc citreum ? the product is composed of mono-, di-, and triglucosylated stevioside and their isomers ? 435838 2.4.1.140 stevioside + sucrose transglucosylation Leuconostoc citreum SK24.002 ? the product is composed of mono-, di-, and triglucosylated stevioside and their isomers ? 435838 2.4.1.140 sucrose + alpha-D-glucan - Leuconostoc mesenteroides alternating-1,6-1,3-alpha-D-glucan - ? 322074 2.4.1.140 sucrose + alpha-D-glucan - Streptococcus mutans alternating-1,6-1,3-alpha-D-glucan the product is an insoluble D-glucan that consists of 76 mol% 1,3-alpha-linked glucose and 24 mol% 1,6-alpha-linked glucose ? 322074 2.4.1.140 sucrose + alpha-D-glucan - Streptococcus mutans alternating-1,6-1,3-alpha-D-glucan glucan consists of 49.1 mol% 1,6-alpha-linked glucose and 33.9 mol% 1,3-alpha-linked glucose with 13.6 mol% terminal glucose and 3.3 mol% 1,3,6-alpha-branched glucose ? 322074 2.4.1.140 sucrose + alpha-D-glucan - Leuconostoc mesenteroides NRRL B-1355 alternating-1,6-1,3-alpha-D-glucan - ? 322074 2.4.1.140 sucrose + alpha-D-glucan - Leuconostoc mesenteroides NRRL B-23192 alternating-1,6-1,3-alpha-D-glucan - ? 322074 2.4.1.140 sucrose + alpha-D-glucan - Leuconostoc mesenteroides NRRL B-21297 alternating-1,6-1,3-alpha-D-glucan - ? 322074 2.4.1.140 sucrose + alpha-D-glucan - Leuconostoc citreum ? - ? 458239 2.4.1.140 sucrose + alpha-D-glucan - Leuconostoc citreum ABK-1 ? - ? 458239 2.4.1.140 sucrose + alpha-D-glucopyranosyl-(1,4)-L-glucose - Leuconostoc mesenteroides ? - ? 377806 2.4.1.140 sucrose + alpha-D-glucopyranosyl-(1,4)-L-glucose - Leuconostoc mesenteroides NRRL B-21297 ? - ? 377806 2.4.1.140 sucrose + butyl alpha-D-glucopyranoside - Leuconostoc mesenteroides ? - ? 322078 2.4.1.140 sucrose + cellobiose - Leuconostoc mesenteroides oligosaccharides alpha-D-glucopyranosyl-(1,2)-(beta-D-glucopyranosyl-(1,4))-D-glucopyranose + alpha-D-glucopyranosyl-(1,6)-beta-D-glucopyranosyl-(1,4)-D-glucopyranose, the last compound in turn can be glycosylated leading to the synthesis of a tetrasaccharide with an additional alpha-(1,6)-linkage at the non-reducing end ? 322080 2.4.1.140 sucrose + cellobiose - Leuconostoc mesenteroides NRRL B-23192 oligosaccharides alpha-D-glucopyranosyl-(1,2)-(beta-D-glucopyranosyl-(1,4))-D-glucopyranose + alpha-D-glucopyranosyl-(1,6)-beta-D-glucopyranosyl-(1,4)-D-glucopyranose, the last compound in turn can be glycosylated leading to the synthesis of a tetrasaccharide with an additional alpha-(1,6)-linkage at the non-reducing end ? 322080 2.4.1.140 sucrose + D-tagatose - Leuconostoc mesenteroides alpha-D-glucopyranosyl-(1,1)-beta-D-tagatopyranose - ? 377801 2.4.1.140 sucrose + D-tagatose - Leuconostoc mesenteroides NRRL B-21297 alpha-D-glucopyranosyl-(1,1)-beta-D-tagatopyranose - ? 377801 2.4.1.140 sucrose + gentiobiose gentiobiose is better as an acceptor than isomaltose, acceptor products from gentiobiose, form in good yields (nearly 90% in unoptimized reactions). The initial product is a single trisaccharide, alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc. Two tetrasaccharides are formed in approximately equal quantities: alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc and alpha-D-Glcp-(1-6)-alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc. One pentasaccharide is isolated from the reaction mixture, alpha-D-Glcp-(1-6)-alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc Leuconostoc mesenteroides ? - ? 410332 2.4.1.140 sucrose + gentiobiose gentiobiose is better as an acceptor than isomaltose, acceptor products from gentiobiose, form in good yields (nearly 90% in unoptimized reactions). The initial product is a single trisaccharide, alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc. Two tetrasaccharides are formed in approximately equal quantities: alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc and alpha-D-Glcp-(1-6)-alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc. One pentasaccharide is isolated from the reaction mixture, alpha-D-Glcp-(1-6)-alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-6)-beta-D-Glcp-(1-6)-D-Glc Leuconostoc mesenteroides NRRL B-21297 ? - ? 410332 2.4.1.140 sucrose + isomaltose - Leuconostoc mesenteroides oligoalternan - ? 322076 2.4.1.140 sucrose + isomaltose - Leuconostoc mesenteroides NRRL B-1355 oligoalternan - ? 322076 2.4.1.140 sucrose + isomaltose - Leuconostoc mesenteroides ? - ? 410336 2.4.1.140 sucrose + isomaltose - Leuconostoc mesenteroides NRRL B-21297 ? - ? 410336 2.4.1.140 sucrose + L-glucose - Leuconostoc mesenteroides alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,4)-L-glucose - ? 377802 2.4.1.140 sucrose + L-glucose - Leuconostoc mesenteroides NRRL B-21297 alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,4)-L-glucose - ? 377802 2.4.1.140 sucrose + leucrose regioselectivity of alternansucrase differs from dextransucrase. Alternansucrase shows greater ability to use leucrose as an acceptor, alternansucrase continues to transfer glucosyl units to leucrose, resulting in some unusual glucosyl-fructose oligosaccharides Leuconostoc mesenteroides ? - ? 410339 2.4.1.140 sucrose + luteolin 8% conversion Leuconostoc mesenteroides luteolin-3'-O-alpha-D-glucopyranoside + luteolin-4'-O-alpha-D-glucopyranoside - ? 389821 2.4.1.140 sucrose + luteolin 8% conversion Leuconostoc mesenteroides NRRL B-512F luteolin-3'-O-alpha-D-glucopyranoside + luteolin-4'-O-alpha-D-glucopyranoside - ? 389821 2.4.1.140 sucrose + luteolin 8% conversion Leuconostoc mesenteroides NRRL B-23192 luteolin-3'-O-alpha-D-glucopyranoside + luteolin-4'-O-alpha-D-glucopyranoside - ? 389821 2.4.1.140 sucrose + maltose - Leuconostoc mesenteroides oligoalternan - ? 322075 2.4.1.140 sucrose + maltose - Leuconostoc mesenteroides oligoalternan panose is the first acceptor product ? 322075 2.4.1.140 sucrose + maltose - Leuconostoc mesenteroides NRRL B-1355 oligoalternan panose is the first acceptor product ? 322075 2.4.1.140 sucrose + maltose - Leuconostoc mesenteroides NRRL B-1355 oligoalternan - ? 322075 2.4.1.140 sucrose + maltose - Leuconostoc mesenteroides ? - ? 377817 2.4.1.140 sucrose + maltose alternansucrase acceptor products from maltose do not contain dextran-type linkage sequences. Instead, the product series is comprised of an alternan-type linkage sequence, with some pairs of consecutive alpha-(1,6) linkages in the evennumbered members of the series. The distribution and sequence of linkages is apparently kinetically controlled. Branch formation is not detected below DP 8. Alternansucrase forms alpha-(1,3) linkages only when the acceptor is a-(1,6)-linked, thereby prohibiting the formation of sequences of alpha-(1,3) linkages. Furthermore, the enzyme appears not to make products containing more than two sequential alpha-(1,6) linkages, thereby prohibiting the formation of dextran-like linkage sequences Leuconostoc mesenteroides ? - ? 377817 2.4.1.140 sucrose + maltose maltose undergoes alpha-1,6 glucosylation alone to give the oligodextran of DP3 (OD3 panose, alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc), which can be further elongated at either the O6 or the O3 position of the nonreducing unit to give the structures OD4 (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc) and OA4 (alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc), respectively. The oligosaccharide OA4 is quick to appear at the beginning of the reaction (1 min) and accumulates at a much higher level than OD4, indicating that panose is preferentially elongated with an alpha-1,3 linkage. A very small peak of OD5 originating from OD4 only appears toward the end of the reaction (after about 30 min) and is not elongated further (no OD6 is found). As soon as the OA4 starts to accumulate, it is efficiently converts to OA5 (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc). The OA5 itself can act as an acceptor for the formation of two OA6s (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-DGlc and alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc) Leuconostoc citreum ? - ? 377817 2.4.1.140 sucrose + maltose maltose undergoes alpha-1,6 glucosylation alone to give the oligodextran of DP3 (OD3 panose, alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc), which can be further elongated at either the O6 or the O3 position of the nonreducing unit to give the structures OD4 (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc) and OA4 (alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc), respectively. The oligosaccharide OA4 is quick to appear at the beginning of the reaction (1 min) and accumulates at a much higher level than OD4, indicating that panose is preferentially elongated with an alpha-1,3 linkage. A very small peak of OD5 originating from OD4 only appears toward the end of the reaction (after about 30 min) and is not elongated further (no OD6 is found). As soon as the OA4 starts to accumulate, it is efficiently converts to OA5 (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc). The OA5 itself can act as an acceptor for the formation of two OA6s (alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-DGlc and alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->3)-alpha-D-Glcp-(1->6)-alpha-D-Glcp-(1->4)-D-Glc) Leuconostoc citreum NRRL B-1355 ? - ? 377817 2.4.1.140 sucrose + maltose - Leuconostoc mesenteroides NRRL B-21297 ? - ? 377817 2.4.1.140 sucrose + maltose alternansucrase acceptor products from maltose do not contain dextran-type linkage sequences. Instead, the product series is comprised of an alternan-type linkage sequence, with some pairs of consecutive alpha-(1,6) linkages in the evennumbered members of the series. The distribution and sequence of linkages is apparently kinetically controlled. Branch formation is not detected below DP 8. Alternansucrase forms alpha-(1,3) linkages only when the acceptor is a-(1,6)-linked, thereby prohibiting the formation of sequences of alpha-(1,3) linkages. Furthermore, the enzyme appears not to make products containing more than two sequential alpha-(1,6) linkages, thereby prohibiting the formation of dextran-like linkage sequences Leuconostoc mesenteroides NRRL B-21297 ? - ? 377817 2.4.1.140 sucrose + methyl alpha-D-allo-pyranoside - Leuconostoc mesenteroides methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-allopyranoside + alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-alpha-D-allopyranoside + beta-D-fructofuranose the product methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-allopyranoside is subsequently glucosylated at position 6 to give rise to the trisaccharide methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-alpha-D-allopyranoside. Higher DP products are observed ? 377818 2.4.1.140 sucrose + methyl alpha-D-galactopyranoside - Leuconostoc mesenteroides methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-galactopyranoside + methyl alpha-D-glucopyranosyl-(1,4)-alpha-D-galactopyranoside + beta-D-fructofuranose two initial products methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-galactopyranoside and methyl alpha-D-glucopyranosyl-(1,4)-alpha-D-galactopyranoside, in a 2.5:1 molar ratio ? 377819 2.4.1.140 sucrose + methyl alpha-D-galactopyranoside - Leuconostoc mesenteroides methyl alpha-D-glucopyranosyl-(1,4)-alpha-D-galactopyranoside + methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-galactopyranoside + beta-D-fructofuranose production of methyl alpha-D-glucopyranosyl-(1, 4)-alpha-D-galactopyranoside and methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-galactopyranoside in the ratio 2.5:1 ? 377820 2.4.1.140 sucrose + methyl alpha-D-mannopyranoside - Leuconostoc mesenteroides methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-mannopyranoside + methyl-3,6-di-O-alpha-D-glucopyranosyl-alpha-D-mannopyranoside + beta-D-fructofuranose the major initial acceptor product is methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-mannopyranoside, but several minor products are also isolated and characterized, including a 3,6-di-O-substituted mannopyranoside ? 377821 2.4.1.140 sucrose + methyl beta-D-galactopyranoside poor substrate Leuconostoc mesenteroides ? - ? 377822 2.4.1.140 sucrose + methyl beta-D-mannopyranoside poor substrate Leuconostoc mesenteroides ? - ? 377823 2.4.1.140 sucrose + methyl-alpha-D-glucoside - Leuconostoc mesenteroides oligoalternan - ? 322077 2.4.1.140 sucrose + methyl-alpha-D-glucoside - Leuconostoc mesenteroides NRRL B-1355 oligoalternan - ? 322077 2.4.1.140 sucrose + methyl-beta-D-glucopyranoside - Leuconostoc mesenteroides methyl beta-isomaltoside + methyl beta-isomaltotrioside + methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-glucopyranosyl-(1,6)-beta-D-glucopyranoside + beta-D-fructofuranose the initial product arising is methyl beta-isomaltoside, which is subsequently glucosylated to yield methyl beta-isomaltotrioside and methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-glucopyranosyl-(1,6)-beta-D-glucopyranoside ? 377824 2.4.1.140 sucrose + myricetin 49% conversion Leuconostoc mesenteroides ? - ? 389824 2.4.1.140 sucrose + myricetin 49% conversion Leuconostoc mesenteroides NRRL B-512F ? - ? 389824 2.4.1.140 sucrose + myricetin 49% conversion Leuconostoc mesenteroides NRRL B-23192 ? - ? 389824 2.4.1.140 sucrose + octyl-alpha-D-glucopyranoside - Leuconostoc mesenteroides ? - ? 322079 2.4.1.140 sucrose + quercetin 4% conversion Leuconostoc mesenteroides ? - ? 389829 2.4.1.140 sucrose + quercetin 4% conversion Leuconostoc mesenteroides NRRL B-512F ? - ? 389829 2.4.1.140 sucrose + quercetin 4% conversion Leuconostoc mesenteroides NRRL B-23192 ? - ? 389829 2.4.1.140 sucrose + raffinose the alternansucrase-catalyzed acceptor reaction with raffinose gives a gravimetric yield of alternan of 9.5% relative to the weight of sucrose, indicating that 20% of the glucosyl units are incorporated into alternan, and 80% into oligosaccharide acceptor products. The main products are the tetrasaccharides alpha-D-Glcp-(1-3)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf and alpha-D-Glcp-(1-4)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf in ratios ranging from 4:1 to 9:1, along with lesser amounts of alpha-D-Glcp-(1-6)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf. Pentasaccharides, hexasaccharides and higher oligosaccharides are also produced Leuconostoc mesenteroides ? - ? 389830 2.4.1.140 sucrose + raffinose the alternansucrase-catalyzed acceptor reaction with raffinose gives a gravimetric yield of alternan of 9.5% relative to the weight of sucrose, indicating that 20% of the glucosyl units are incorporated into alternan, and 80% into oligosaccharide acceptor products. The main products are the tetrasaccharides alpha-D-Glcp-(1-3)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf and alpha-D-Glcp-(1-4)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf in ratios ranging from 4:1 to 9:1, along with lesser amounts of alpha-D-Glcp-(1-6)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf. Pentasaccharides, hexasaccharides and higher oligosaccharides are also produced Leuconostoc mesenteroides NRRL B-21297 ? - ? 389830