2.3.1.B41	additional information	insignificant activity with SKEYFS(N6-succinyl)KQK	Archaeoglobus fulgidus	?	-	?	89	
2.3.1.B41	additional information	no activity with KGLGKGGA(N6-succinyl)KRHRKW	Archaeoglobus fulgidus	?	-	?	89	
2.3.1.B41	additional information	no or very low activity with QTAR(N6-acetyl)KSTGG, QTAR(N6-propionyl)KSTGG, QTAR(N6-succinyl)KSTGG, QTAR(N6-butyryl)KSTGG, QTAR(N6-crotonyl)KSTGG	Homo sapiens	?	-	?	89	
2.3.1.B41	additional information	Sirt6 prefers long chain fatty acyl groups. Low deacetylase activity on histone H3 K9 and histone H3 K56. Selectivity for peptide sequence is not high	Homo sapiens	?	-	?	89	
2.3.1.B41	additional information	the enzyme preferentially hydrolyzes long-chain fatty acyl groups over acetyl groups in vitro	Homo sapiens	?	-	?	89	
2.3.1.B41	additional information	SIRT6 forms a complex with MutY homolog, apurinic/apyrimidinic-endonuclease 1, and 9-1-1 checkpoint clamp to maintain genomic and telomeric integrity. SIRT6 enhances the activities of MutY homolog, apurinic/apyrimidinic-endonuclease 1. Human MutY homolog and SIRT6 are efficiently recruited to confined oxidative DNA damage within transcriptionally active chromatin, but not in inactive chromatin	Mus musculus	?	-	?	89	
2.3.1.B41	additional information	acetylation of nicotinamide phosphoribosyltransferase, i.e. NAMPT, on residue K53 by SIRT6	Homo sapiens	?	-	-	89	
2.3.1.B41	additional information	SIRT6 deacetylates Beclin-1 in HCC cells	Homo sapiens	?	-	-	89	
2.3.1.B41	additional information	SIRT6 is a specific deacetylase for H3K9, see EC 2.3.1.286. It also deacetylates H3K56 and H3K18, as well as KAP1, CtIP, PGC-1alpha, TRF2, GCN5, SNF2H, and PKM2	Mus musculus	?	-	-	89	
2.3.1.B41	additional information	SIRT6 is a specific deacetylase for H3K9, see EC 2.3.1.286. It also deacetylates H3K56 and H3K18, as well as KAP1, CtIP, PGC-1alpha, TRF2, GCN5, SNF2H, and PKM2, cf. EC 2.3.1.286	Homo sapiens	?	-	-	89	
2.3.1.B41	additional information	sirtuin6 performs H3K9 acetylation on histone H3, cf. EC 2.3.1.286	Homo sapiens	?	-	-	89	
2.3.1.B41	additional information	a myristoyl peptide and a palmitoyl peptide are incubated with sirtuin6. Sirtuin6 exhibits both SIRT6 deacetylase and deacylase activities, cf. EC 2.3.1.286	Homo sapiens	?	-	-	89	
2.3.1.B41	additional information	SIRT6 can undergo intramolecular mono-ADP-ribosylation utilizing NAD+ as a substrate, i.e. mono-ADP-ribosylation. SIRT6 also performs ADP-ribosyl transferase activity on other protein substrates, overview. Weak deacetylase activity of SIRT6 in vitro	Homo sapiens	?	-	-	89	
2.3.1.B41	additional information	SIRT6 can undergo intramolecular mono-ADP-ribosylation utilizing NAD+ as a substrate. Purified mouse SIRT6 successfully catalyzes the transfer of radiolabel from [32P]NAD+ onto itself, i.e. mono-ADP-ribosylation. also performs ADP-ribosyl transferase activity on other protein substrates, overview. Weak deacetylase activity of SIRT6 in vitro	Mus musculus	?	-	-	89	
2.3.1.B41	additional information	Sirt6-dependent deacetylation of H3K18 and H3K9 in a histone protein preparation and in HeLa nucleosomes	Homo sapiens	?	-	-	89	
2.3.1.B41	NAD+ + Ac-AK(N6-acetyl)K-7-amido-4-methylcoumarin	the substrate is based on the human tumour suppressor protein p53	Homo sapiens	nicotinamide + Ac-AKK-7-amido-4-methylcoumarin + 2'-O-acetyl-ADP-ribose	-	?	430456	
2.3.1.B41	NAD+ + Ac-HK(N6-acetyl)K-7-amido-4-methylcoumarin	the substrate is based on the human tumour suppressor protein p53	Homo sapiens	nicotinamide + Ac-HKK-7-amido-4-methylcoumarin + 2'-O-acetyl-ADP-ribose	-	?	430457	
2.3.1.B41	NAD+ + Ac-RHK(N6-acetyl)K-7-amido-4-methylcoumarin	the substrate is based on the amino acids 379-382 of the human tumour suppressor protein p53	Homo sapiens	nicotinamide + Ac-RHKK-7-amido-4-methylcoumarin + 2'-O-acetyl-ADP-ribose	-	?	430460	
2.3.1.B41	NAD+ + Ac-RYQ(N6-acetyl)K-7-amido-4-methylcoumarin	the peptide substrate mimics the biological deacetylation site of histone H3 K56	Homo sapiens	nicotinamide + Ac-RYQK-7-amido-4-methylcoumarin + 2'-O-acetyl-ADP-ribose	-	?	430461	
2.3.1.B41	NAD+ + Ac-TAR(N6-acetyl)K-7-amido-4-methylcoumarin	the peptide substrate mimics the biological deacetylation site of histone H3 K9	Homo sapiens	nicotinamide + Ac-TARK-7-amido-4-methylcoumarin + 2'-O-acetyl-ADP-ribose	-	?	430462	
2.3.1.B41	NAD+ + KGLGKGGA(N6-acetyl)KRHRKW	the enzyme shows increased reaction velocity with increasing acyl chain length. As compared to its deacetylating activity, Sir2Af2 depropionylates, debutyrylates, and demyristoylates a peptide of the same sequence at 1.5-, 1.9-, and 3.4-fold higher rates, respectively	Archaeoglobus fulgidus	nicotinamide + KGLGKGGAKRHRKW + 2'-O-acetyl-ADP-ribose	-	?	430477	
2.3.1.B41	NAD+ + KGLGKGGA(N6-butyryl)KRHRKW	the enzyme shows increased reaction velocity with increasing acyl chain length. As compared to its deacetylating activity, Sir2Af2 depropionylates, debutyrylates, and demyristoylates a peptide of the same sequence at 1.5-, 1.9-, and 3.4-fold higher rates, respectively	Archaeoglobus fulgidus	nicotinamide + KGLGKGGAKRHRKW + 2'-O-butyryl-ADP-ribose	-	?	430478	
2.3.1.B41	NAD+ + KGLGKGGA(N6-myristoyl)KRHRKW	the enzyme shows increased reaction velocity with increasing acyl chain length. As compared to its deacetylating activity, Sir2Af2 depropionylates, debutyrylates, and demyristoylates a peptide of the same sequence at 1.5-, 1.9-, and 3.4-fold higher rates, respectively	Archaeoglobus fulgidus	nicotinamide + KGLGKGGAKRHRKW + 2'-O-myristoyl-ADP-ribose	-	?	430479	
2.3.1.B41	NAD+ + KGLGKGGA(N6-propionyl)KRHRKW	the enzyme shows increased reaction velocity with increasing acyl chain length. As compared to its deacetylating activity, Sir2Af2 depropionylates, debutyrylates, and demyristoylates a peptide of the same sequence at 1.5-, 1.9-, and 3.4-fold higher rates, respectively	Archaeoglobus fulgidus	nicotinamide + KGLGKGGAKRHRKW + 2'-O-propionyl-ADP-ribose	-	?	430480	
2.3.1.B41	NAD+ + QTAR(N6-decanoyl)KSTGG	-	Homo sapiens	nicotinamide + QTARKSTGG + 2'-O-decanoyl-ADP-ribose	-	?	430493	
2.3.1.B41	NAD+ + QTAR(N6-decanoyl)KSTGG	myristoylated histone H3 peptide, about 60% compared to the activity with the decanoylated peptide	Homo sapiens	nicotinamide + QTARKSTGG + 2'-O-decanoyl-ADP-ribose	-	?	430493	
2.3.1.B41	NAD+ + QTAR(N6-dodecanoyl)KSTGG	dodecanoylated histone H3 peptide, about 60% compared to the activity with the decanoylated peptide	Homo sapiens	nicotinamide + QTARKSTGG + 2'-O-dodecanoyl-ADP-ribose	-	?	430494	
2.3.1.B41	NAD+ + QTAR(N6-hexanoyl)KSTGG	hexanoylated histone H3 peptide, about 20% compared to the activity with the decanoylated peptide	Homo sapiens	nicotinamide + QTARKSTGG + 2'-O-hexanoyl-ADP-ribose	-	?	430495	
2.3.1.B41	NAD+ + QTAR(N6-octanoyl)KSTGG	octanoylated histone H3 peptide, about 50% compared to the activity with the decanoylated peptide	Homo sapiens	nicotinamide + QTARKSTGG + 2'-O-octanoyl-ADP-ribose	-	?	430499	
2.3.1.B41	NAD+ + SKEYFS(N6-acetyl)KQK	-	Archaeoglobus fulgidus	nicotinamide + SKEYFSKQK + 2'-O-acetyl-ADP-ribose	-	?	430505	
2.3.1.B41	NAD+ + [histone 3]-N6-acetyl-L-lysine9	-	Homo sapiens	nicotinamide + [histone 3]-L-lysine + 2'-O-acetyl-ADP ribose	-	?	437756	
2.3.1.B41	NAD+ + [histone 3]-N6-palmitoyl-L-lysine	-	Homo sapiens	nicotinamide + [histone 3]-L-lysine + 2'-O-palmitoyl-ADP ribose	-	?	436523	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-acetyl-L-lysine	-	Homo sapiens	nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-acetyl-ADP-ribose	-	?	430519	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-acetyl-L-lysine	the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups	Plasmodium falciparum	nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-acetyl-ADP-ribose	-	?	430519	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-acetyl-L-lysine	the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups	Plasmodium falciparum isolate 3D7	nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-acetyl-ADP-ribose	-	?	430519	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-acetyl-L-lysine18	-	Homo sapiens	nicotinamide + [histone H3 peptide]-L-lysine18 + 2'-O-acetyl-ADP-ribose	-	?	454434	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-acetyl-L-lysine9	-	Homo sapiens	nicotinamide + [histone H3 peptide]-L-lysine9 + 2'-O-acetyl-ADP-ribose	-	?	454435	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-butyryl-L-lysine	-	Homo sapiens	nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-butyryl-ADP-ribose	-	?	430520	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-butyryl-L-lysine	the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups	Plasmodium falciparum	nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-butyryl-ADP-ribose	-	?	430520	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-butyryl-L-lysine	the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups	Plasmodium falciparum isolate 3D7	nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-butyryl-ADP-ribose	-	?	430520	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-myristoyl-L-lysine	-	Homo sapiens	nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-myristoyl-ADP-ribose	-	?	430521	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-myristoyl-L-lysine	the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups	Plasmodium falciparum	nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-myristoyl-ADP-ribose	-	?	430521	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-myristoyl-L-lysine	the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups	Plasmodium falciparum isolate 3D7	nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-myristoyl-ADP-ribose	-	?	430521	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-octanoyl-L-lysine	-	Homo sapiens	nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-octanoyl-ADP-ribose	-	?	430522	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-octanoyl-L-lysine	the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups	Plasmodium falciparum	nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-octanoyl-ADP-ribose	-	?	430522	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-octanoyl-L-lysine	the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups	Plasmodium falciparum isolate 3D7	nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-octanoyl-ADP-ribose	-	?	430522	
2.3.1.B41	NAD+ + [histone H3 peptide]-N6-palmitoyl-L-lysine	-	Homo sapiens	nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-palmitoyl-ADP-ribose	-	?	430523	
2.3.1.B41	NAD+ + [histone H3]-N6-acetyl-L-lysine	-	Homo sapiens	nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP-ribose	-	?	429330	
2.3.1.B41	NAD+ + [histone H3]-N6-acetyl-L-lysine	-	Plasmodium falciparum	nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP-ribose	-	?	429330	
2.3.1.B41	NAD+ + [histone H3]-N6-acetyl-L-lysine	the enzyme deacetylates histones H3 and H4 when they are packaged as nucleosomes, but not as free histones	Homo sapiens	nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP-ribose	-	?	429330	
2.3.1.B41	NAD+ + [histone H3]-N6-acetyl-L-lysine	-	Plasmodium falciparum isolate 3D7	nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP-ribose	-	?	429330	
2.3.1.B41	NAD+ + [histone H3]-N6-acetyl-L-lysine	-	Homo sapiens	nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP ribose	-	?	437760	
2.3.1.B41	NAD+ + [histone H3]-N6-acetyl-L-lysine18	-	Homo sapiens	nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP ribose	-	?	436524	
2.3.1.B41	NAD+ + [histone H3]-N6-acetyl-L-lysine56	-	Mus musculus	nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP ribose	-	?	436525	
2.3.1.B41	NAD+ + [histone H3]-N6-acetyl-L-lysine9	-	Homo sapiens	nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP ribose	-	?	436526	
2.3.1.B41	NAD+ + [histone H3]-N6-acetyl-L-lysine9	-	Mus musculus	nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP ribose	-	?	436526	
2.3.1.B41	NAD+ + [histone H3]-N6-myristoyl-L-lysine	-	Homo sapiens	nicotinamide + [histone H3]-L-lysine + 2'-O-myristoyl-ADP ribose	-	?	437762	
2.3.1.B41	NAD+ + [histone H3]-N6-palmitoyl-L-lysine	-	Homo sapiens	nicotinamide + [histone H3]-L-lysine + 2'-O-palmitoyl-ADP ribose	-	?	437763	
2.3.1.B41	NAD+ + [histone H3]-N6-palmitoyl-L-lysine9	-	Homo sapiens	nicotinamide + [histone H3]-L-lysine + 2'-O-palmitoyl-ADP ribose	-	?	436527	
2.3.1.B41	NAD+ + [histone H4]-N6-acetyl-L-lysine	-	Homo sapiens	nicotinamide + [histone H4]-L-lysine + 2'-O-acetyl-ADP-ribose	-	?	429332	
2.3.1.B41	NAD+ + [histone H4]-N6-acetyl-L-lysine	-	Plasmodium falciparum	nicotinamide + [histone H4]-L-lysine + 2'-O-acetyl-ADP-ribose	-	?	429332	
2.3.1.B41	NAD+ + [histone H4]-N6-acetyl-L-lysine	the enzyme deacetylates histones H3 and H4 when they are packaged as nucleosomes, but not as free histones	Homo sapiens	nicotinamide + [histone H4]-L-lysine + 2'-O-acetyl-ADP-ribose	-	?	429332	
2.3.1.B41	NAD+ + [histone H4]-N6-acetyl-L-lysine	-	Plasmodium falciparum isolate 3D7	nicotinamide + [histone H4]-L-lysine + 2'-O-acetyl-ADP-ribose	-	?	429332	
2.3.1.B41	NAD+ + [Per2]-N6-acetyl-L-lysine	-	Mus musculus	nicotinamide + [Per2]-L-lysine + 2'-O-acetyl-ADP ribose	-	?	454437	
2.3.1.B41	NAD+ + [protein]-N6-palmitoyl-L-lysine	-	Homo sapiens	nicotinamide + [protein]-L-lysine + 2'-O-palmitoyl-ADP ribose	-	?	436530	
2.3.1.B41	NAD+ + [protein]-N6-palmitoyl-L-lysine	-	Mus musculus	nicotinamide + [protein]-L-lysine + 2'-O-palmitoyl-ADP ribose	-	?	436530	
2.3.1.B41	NAD+ + [protein]-N6-palmitoyl-L-lysine	-	Bos taurus	nicotinamide + [protein]-L-lysine + 2'-O-palmitoyl-ADP ribose	-	?	436530	
2.3.1.B41	NAD+ + [synthetic tumor necrosis factor alpha peptide]-N6-myristoyl-L-lysine19	-	Homo sapiens	nicotinamide + [synthetic tumor necrosis factor alpha peptide]-L-lysine19 + 2'-O-myristoyl-ADP-ribose	-	?	430530	
2.3.1.B41	NAD+ + [synthetic tumor necrosis factor alpha peptide]-N6-myristoyl-L-lysine20	-	Homo sapiens	nicotinamide + [synthetic tumor necrosis factor alpha peptide]-L-lysine20 + 2'-O-myristoyl-ADP-ribose	-	?	430531	
2.3.1.B41	NAD+ + [TNF-alpha]-N6-palmitoyl-L-lysine	-	Homo sapiens	nicotinamide + [TNF-alpha]-L-lysine + 2'-O-palmitoyl-ADP ribose	-	?	454439	
2.3.1.B41	NAD+ + [TNF-alpha]-N6-palmitoyl-L-lysine	-	Mus musculus	nicotinamide + [TNF-alpha]-L-lysine + 2'-O-palmitoyl-ADP ribose	-	?	454439	
2.3.1.B41	NAD+ + [tumor necrosis factor alpha]-N6-acyl-L-lysine20	SIRT6 promotes the secretion of tumor necrosis factor alpha by removing the fatty acyl modification on K19 and K20 of TNFalpha	Homo sapiens	nicotinamide + [tumor necrosis factor alpha]-L-lysine20 + 2'-O-acyl-ADP-ribose	-	?	429350	
2.3.1.B41	NAD+ + [tumor necrosis factor alpha]-N6-myristoyl-L-lysine	-	Homo sapiens	nicotinamide + [tumor necrosis factor alpha]-L-lysine + 2'-O-myristoyl-ADP-ribose	-	?	430532	
2.3.1.B41	NAD+ + [tumor necrosis factor-alpha]-N6-acetyl-L-lysine9	-	Homo sapiens	nicotinamide + [tumor necrosis factor-alpha]-L-lysine + 2'-O-acetyl-ADP ribose	-	?	437766