2.3.1.286 2 KKGQSTSRHKKAcLMFKTEG + 2 NAD+ + 2 H2O - Thermotoga maritima KKGQSTSRHKKLMFKTEG + 2''-O-acetyl-ADP-D-ribose + 3''-O-acetyl-ADP-D-ribose + nicotinamide - ? 447641 2.3.1.286 acetyl-CoA + [protein]-L-lysine - Escherichia coli CoA + [protein]-N6-acetyl-L-lysine - ? 454303 2.3.1.286 acetyl-cytochrome c + NAD+ + H2O the enzyme does not deacetylate native acetyl-cytochrome c but does deacetylate the heat-denatured substrate Saccharomyces cerevisiae ? - ? 446931 2.3.1.286 acetyl-poly-L-lysine + NAD+ + H2O - Saccharomyces cerevisiae ? - ? 446932 2.3.1.286 AGG(AcK)GG(AcK)GMG(AcK)VGA(AcK)RHSC + NAD+ + H2O tetraacetylated histone-H4 N-terminal tail peptide Saccharomyces cerevisiae AGG(AcK)GG(AcK)GMG(AcK)VGAKRHSC + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446947 2.3.1.286 ARTKQTAR(AcK)STGG(AcK)APRKQLC + NAD+ + H2O diacetyl ated histone-H3 N-terminal tail peptide Saccharomyces cerevisiae ARTKQTARKSTGGKAPRKQLC + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 447009 2.3.1.286 fluorogenic acetyl-L-lysine + NAD+ + H2O - Saccharomyces cerevisiae ? - ? 447528 2.3.1.286 HLKSKKGQSTSRHK(K-Ac)LMFK + NAD+ + H2O p53A peptide Archaeoglobus fulgidus HLKSKKGQSTSRHKKLMFK + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 447602 2.3.1.286 hyperacetylated histone-DNA complex + NAD+ + H2O - Drosophila melanogaster deacetylated nuclease-resistant histone-DNA complex + ? - ? 446283 2.3.1.286 K382azaKAc-containing p53 peptide + NAD+ + H2O - Thermotoga maritima K382azaK-containing p53 peptide + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 447635 2.3.1.286 K382KAc-containing p53 peptide + NAD+ + H2O - Thermotoga maritima K382K-containing p53 peptide + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 447636 2.3.1.286 KKGQSTSRHKKAcLMFKTEG + NAD+ + H2O - Mus musculus KKGQSTSRHKKLMFKTEG + 2''-O-acetyl-ADP-D-ribose + nicotinamide - r 447642 2.3.1.286 KKGQSTSRHKKAcLMFKTEG + NAD+ + H2O - Saccharomyces cerevisiae KKGQSTSRHKKLMFKTEG + 2''-O-acetyl-ADP-D-ribose + nicotinamide - r 447642 2.3.1.286 KKGQSTSRHKKAcLMFKTEG + NAD+ + H2O - Archaeoglobus fulgidus KKGQSTSRHKKLMFKTEG + 2''-O-acetyl-ADP-D-ribose + nicotinamide - r 447642 2.3.1.286 KKGQSTSRHKLMFKTEG + 2''-O-acetyl-ADP-D-ribose + nicotinamide - Mus musculus KKGQSTSRHKKAcLMFKTEG + NAD+ + H2O - r 447643 2.3.1.286 KKGQSTSRHKLMFKTEG + 2''-O-acetyl-ADP-D-ribose + nicotinamide - Saccharomyces cerevisiae KKGQSTSRHKKAcLMFKTEG + NAD+ + H2O - r 447643 2.3.1.286 KKGQSTSRHKLMFKTEG + 2''-O-acetyl-ADP-D-ribose + nicotinamide - Archaeoglobus fulgidus KKGQSTSRHKKAcLMFKTEG + NAD+ + H2O - r 447643 2.3.1.286 KSTGGAcKAPRKQ + NAD+ + H2O - Saccharomyces cerevisiae KSTGGKAPRKQ + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 447654 2.3.1.286 KSTGGK(Ac)APRKQ + beta-2'-deoxy-2'-fluororibo-NAD+ + H2O the rates with beta-2'-deoxy-2'-fluororibo-NAD+ are about 200fold slower than the exchange rates determined with NAD+ under similar conditions Saccharomyces cerevisiae ? - ? 447655 2.3.1.286 KSTGGK(Ac)APRKQ + beta-2'-deoxy-2'-fluororibo-NAD+ + H2O the rates with beta-2'-deoxy-2'-fluororibo-NAD+ are about 1200fold slower than the exchange rates determined with NAD+ under similar conditions Homo sapiens ? - ? 447655 2.3.1.286 KSTGGK(Ac)APRKQ + NAD+ + H2O an 11-mer histone H3 peptide Homo sapiens KSTGGKAPRKQ + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 447656 2.3.1.286 KSTGGK(Ac)APRKQ + NAD+ + H2O an 11-mer histone H3 peptide Saccharomyces cerevisiae KSTGGKAPRKQ + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 447656 2.3.1.286 additional information no activity with the crotonylated histone H3 peptide QTAR(N6-crotonyl)KSTGG Homo sapiens ? - ? 89 2.3.1.286 additional information no activity with the succinylated histone H3 peptide QTAR(N6-succinyl)KSTGG Homo sapiens ? - ? 89 2.3.1.286 additional information Sirt3 has almost no activity against acetylated cytochrome c Homo sapiens ? - ? 89 2.3.1.286 additional information the enzyme is specific for acetyl-lysine within proteins. It does not deacetylate small polycations such as acetyl-spermine or acetyl-protamine or acetylated amino termini of proteins. Furthermore, the enzyme displays conformational rather than sequence specificity, preferentially deacetylating acetyl-lysine within unstructured regions of proteins Saccharomyces cerevisiae ? - ? 89 2.3.1.286 additional information the enzyme preferentially hydrolyzes medium and long chain fatty acyl lysine. The deacetylase activity of the enzyme is weak Plasmodium falciparum ? - ? 89 2.3.1.286 additional information Nepsilon-lysine acetyltransferase PhnO specifically transfer an acetyl group from AcCoA to Nepsilon-lysine residues on proteins. The enzyme shows a high degree of substrate specificity Escherichia coli ? - - 89 2.3.1.286 additional information determination of substrate specificity, method, detailed overview. Structural analysis of KAT and AcP-dependent acetylation sites Escherichia coli ? - - 89 2.3.1.286 additional information the enzyme also acts as an aminoalkylphosphonate N-acetyltransferase, EC 2.3.1.380. Determination of substrate specificity, method, detailed overview. Structural analysis of KAT and AcP-dependent acetylation sites Escherichia coli ? - - 89 2.3.1.286 NAD+ + L-Arg-L-His-L-Lys-L-Lys(epsilon-acetyl)-7-amido-4-methylcoumarin - Rattus norvegicus nicotinamide + ? - ? 437752 2.3.1.286 NAD+ + N2-benzyloxycarbonyl-N6-succinyl-L-lysine-7-amido-4-methylcoumarin i.e. 3-[(5-[[(benzyloxy)carbonyl]amino]-5-[(4-methyl-2-oxo-2H-chromen-7-yl)carbamoyl]pentyl)carbamoyl]-propanoic acid Homo sapiens nicotinamide + N2-benzyloxycarbonyl-L-lysine-7-amido-4-methylcoumarin + 2'-O-succinyl-ADP-ribose - ? 430487 2.3.1.286 NAD+ + N2-benzyloxycarbonyl-N6-succinyl-L-lysine-7-amido-4-methylcoumarin i.e. 3-[(5-[[(benzyloxy)carbonyl]amino]-5-[(4-methyl-2-oxo-2H-chromen-7-yl)carbamoyl]pentyl)carbamoyl]-propanoic acid Homo sapiens nicotinamide + N2-benzyloxycarbonyl-L-lysine-7-amido-4-methylcoumarin + 2'-O-succinyl-ADP ribose - ? 430488 2.3.1.286 NAD+ + QTAR(N6-acetyl)KSTGG acetylated histone H3 peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-acetyl-ADP-ribose - ? 430490 2.3.1.286 NAD+ + QTAR(N6-butyryl)KSTGG butyrylated histone H3 peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-butyryl-ADP-ribose - ? 430491 2.3.1.286 NAD+ + QTAR(N6-butyryl)KSTGG butyrylated histone H3 peptide, about 20% compared to the activity with the acetylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-butyryl-ADP-ribose - ? 430491 2.3.1.286 NAD+ + QTAR(N6-butyryl)KSTGG butyrylated histone H3 peptide, about 5% compared to the activity with the acetylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-butyryl-ADP-ribose - ? 430491 2.3.1.286 NAD+ + QTAR(N6-crotonyl)KSTGG crotonylated histone H3 peptide, about 20% compared to the activity with the acetylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-crotonyl-ADP-ribose - ? 430492 2.3.1.286 NAD+ + QTAR(N6-crotonyl)KSTGG crotonylated histone H3 peptide, about 30% compared to the activity with the acetylated or hexanoylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-crotonyl-ADP-ribose - ? 430492 2.3.1.286 NAD+ + QTAR(N6-decanoyl)KSTGG decanoylated histone H3 peptide, about 50% compared to the activity with the acetylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-decanoyl-ADP-ribose - ? 430493 2.3.1.286 NAD+ + QTAR(N6-decanoyl)KSTGG decanoylated histone H3 peptide, about 65% compared to the activity with the acetylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-decanoyl-ADP-ribose - ? 430493 2.3.1.286 NAD+ + QTAR(N6-decanoyl)KSTGG decanoylated histone H3 peptide, about 80% compared to the activity with the acetylated or hexanoylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-decanoyl-ADP-ribose - ? 430493 2.3.1.286 NAD+ + QTAR(N6-decanoyl)KSTGG myristoylated histone H3 peptide, about 50% compared to the activity with the acetylated or hexanoylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-decanoyl-ADP-ribose - ? 430493 2.3.1.286 NAD+ + QTAR(N6-decanoyl)KSTGG myristoylated histone H3 peptide, about 65% compared to the activity with the acetylated or hexanoylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-decanoyl-ADP-ribose - ? 430493 2.3.1.286 NAD+ + QTAR(N6-dodecanoyl)KSTGG dodecanoylated histone H3 peptide, about 30% compared to the activity with the acetylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-dodecanoyl-ADP-ribose - ? 430494 2.3.1.286 NAD+ + QTAR(N6-dodecanoyl)KSTGG dodecanoylated histone H3 peptide, about 60% compared to the activity with the acetylated or hexanoylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-dodecanoyl-ADP-ribose - ? 430494 2.3.1.286 NAD+ + QTAR(N6-dodecanoyl)KSTGG dodecanoylated histone H3 peptide, about 65% compared to the activity with the acetylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-dodecanoyl-ADP-ribose - ? 430494 2.3.1.286 NAD+ + QTAR(N6-hexanoyl)KSTGG hexanoylated histone H3 peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-hexanoyl-ADP-ribose - ? 430495 2.3.1.286 NAD+ + QTAR(N6-hexanoyl)KSTGG hexanoylated histone H3 peptide, about 20% compared to the activity with the acetylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-hexanoyl-ADP-ribose - ? 430495 2.3.1.286 NAD+ + QTAR(N6-hexanoyl)KSTGG hexanoylated histone H3 peptide, about 40% compared to the activity with the acetylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-hexanoyl-ADP-ribose - ? 430495 2.3.1.286 NAD+ + QTAR(N6-myristoyl)KSTGG myristoylated histone H3 peptide, about 45% compared to the activity with the acetylated or hexanoylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-myristoyl-ADP-ribose - ? 430498 2.3.1.286 NAD+ + QTAR(N6-octanoyl)KSTGG octanoylated histone H3 peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-octanoyl-ADP-ribose - ? 430499 2.3.1.286 NAD+ + QTAR(N6-octanoyl)KSTGG octanoylated histone H3 peptide, about 35% compared to the activity with the acetylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-octanoyl-ADP-ribose - ? 430499 2.3.1.286 NAD+ + QTAR(N6-octanoyl)KSTGG octanoylated histone H3 peptide, about 55% compared to the activity with the acetylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-octanoyl-ADP-ribose - ? 430499 2.3.1.286 NAD+ + QTAR(N6-palmitoyl)KSTGG myristoylated histone H3 peptide, about 30% compared to the activity with the acetylated or hexanoylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-palmitoyl-ADP-ribose - ? 430500 2.3.1.286 NAD+ + QTAR(N6-propionyl)KSTGG propionylated histone H3 peptide, about 10% compared to the activity with the acetylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-propionyl-ADP-ribose - ? 430501 2.3.1.286 NAD+ + QTAR(N6-propionyl)KSTGG propionylated histone H3 peptide, about 40% compared to the activity with the acetylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-propionyl-ADP-ribose - ? 430501 2.3.1.286 NAD+ + QTAR(N6-propionyl)KSTGG propionylated histone H3 peptide, about 60% compared to the activity with the acetylated or hexanoylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-propionyl-ADP-ribose - ? 430501 2.3.1.286 NAD+ + QTAR(N6-succinyl)KSTGG succinylated histone H3 peptide, about 10% compared to the activity with the acetylated peptide Homo sapiens nicotinamide + QTARKSTGG + 2'-O-succinyl-ADP-ribose - ? 430502 2.3.1.286 NAD+ + TRSG(N6-acetyl)KVMR peptide based on an acetyl-CoA synthetase 2 acetylation site Homo sapiens nicotinamide + TRSGKVMR + 2'-O-acetyl-ADP-ribose - ? 430534 2.3.1.286 NAD+ + TRSG(N6-acetyl)KVMRRLLR acetylated substrate peptide based on the sequence of acetyl-coenzyme A synthetase 2 Homo sapiens nicotinamide + TRSGKVMRRLLR + 2'-O-acetyl-ADP-ribose - ? 430509 2.3.1.286 NAD+ + [alpha-tubulin peptide]-N6-acetyl-L-lysine - Homo sapiens nicotinamide + [alpha-tubulin peptide]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 430511 2.3.1.286 NAD+ + [alpha-tubulin]-N6-acetyl-L-lysine40 - Homo sapiens nicotinamide + [alpha-tubulin]-L-lysine40 + 2'-O-acetyl-ADP ribose - ? 430512 2.3.1.286 NAD+ + [alpha-tubulin]-N6-acyl-L-lysine - Rattus norvegicus nicotinamide + [alpha-tubulin]-L-lysine + 2'-O-acyl-ADP-ribose - ? 436516 2.3.1.286 NAD+ + [alpha-tubulin]-N6-acyl-L-lysine - Homo sapiens nicotinamide + [alpha-tubulin]-L-lysine + 2'-O-acyl-ADP-ribose - ? 436516 2.3.1.286 NAD+ + [BHJH-TM1 protein]-N6-myristoyl-L-lysine - Homo sapiens nicotinamide + [BHJH-TM1 protein]-L-lysine + 2'-O-myristoyl-ADP-ribose - ? 437753 2.3.1.286 NAD+ + [BMAL1 protein]-N6-acyl-L-lysine - Rattus norvegicus nicotinamide + [BMAL1 protein]-L-lysine + 2'-O-acyl-ADP-ribose - ? 436517 2.3.1.286 NAD+ + [BMAL1 protein]-N6-acyl-L-lysine - Homo sapiens nicotinamide + [BMAL1 protein]-L-lysine + 2'-O-acyl-ADP-ribose - ? 436517 2.3.1.286 NAD+ + [chicken erythrocyte histone]-N6-acetyl-L-lysine - Homo sapiens nicotinamide + [protein]-L-lysine + 2'-O-acetyl-ADP ribose - ? 429326 2.3.1.286 NAD+ + [DNA repair factor Ku70]-N6-acetyl-L-lysine382 - Homo sapiens nicotinamide + [DNA repair factor Ku70]-L-lysine382 + 2'-O-acetyl-ADP-ribose - ? 429328 2.3.1.286 NAD+ + [DNA repair factor Ku70]-N6-acetyl-L-lysine382 - Rattus norvegicus nicotinamide + [DNA repair factor Ku70]-L-lysine382 + 2'-O-acetyl-ADP-ribose - ? 429328 2.3.1.286 NAD+ + [DNA repair factor Ku70]-N6-acetyl-L-lysine382 SIRT1 modulates DNA repair activity, which can be regulated by the acetylation status of repair protein Ku70 following DNA damage Homo sapiens nicotinamide + [DNA repair factor Ku70]-L-lysine382 + 2'-O-acetyl-ADP-ribose - ? 429328 2.3.1.286 NAD+ + [Fluor de Lys protein]-N6-acyl-L-lysine - Rattus norvegicus nicotinamide + [Fluor de Lys protein]-L-lysine + 2'-O-acyl-ADP-ribose - ? 436518 2.3.1.286 NAD+ + [Fluor de Lys]-N6-acetyl-L-lysine - Mus musculus nicotinamide + [Fluor de Lys]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 430516 2.3.1.286 NAD+ + [Fluor de Lys]-N6-acetyl-L-lysine Fluor de Lys is a fluorogenic, acetylated peptide substrate for human sirtuin 1, based on residues 379-382 of p53 (Arg-His-Lys-Lys(Ac)), a site of regulatory acetylation by the p300 and CBP acetyltransferases Homo sapiens nicotinamide + [Fluor de Lys]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 430516 2.3.1.286 NAD+ + [glutamate dehydrogenase]-N6-acetyl-L-lysine - Homo sapiens nicotinamide + [glutamate dehydrogenase]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 429329 2.3.1.286 NAD+ + [glutamate dehydrogenase]-N6-acetyl-L-lysine Sirt3 can deacetylate and thereby activate a central metabolic regulator in the mitochondrial matrix, glutamate dehydrogenase Homo sapiens nicotinamide + [glutamate dehydrogenase]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 429329 2.3.1.286 NAD+ + [glutamate oxaloacetate transaminase 2]-N6-acetyl-L-lysine isoform SIRT3 is the major deacetylase of glutamate oxaloacetate transaminase 2 Homo sapiens nicotinamide + [glutamate oxaloacetate transaminase 2]-L-lysine + 2'-O-acyl-ADP-ribose - ? 436521 2.3.1.286 NAD+ + [heat shock protein 10]-N6-acetyl-L-lysine56 - Mus musculus nicotinamide + [heat shock protein 10]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 436522 2.3.1.286 NAD+ + [histone H2B]-N6-acetyl-L-lysine12 - Homo sapiens nicotinamide + [histone H2B]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 437757 2.3.1.286 NAD+ + [histone H2B]-N6-myristoyl-L-lysine12 - Homo sapiens nicotinamide + [histone H2B]-L-lysine + 2'-O-myristoyl-ADP-ribose - ? 437758 2.3.1.286 NAD+ + [histone H3]-N6-acetyl-L-lysine9 - Homo sapiens nicotinamide + [histone H3]-L-lysine9 + 2'-O-acetyl-ADP-ribose - ? 430524 2.3.1.286 NAD+ + [histone H3]-N6-acetyl-L-lysine9 deacetylated at high concentrations of enzyme Homo sapiens nicotinamide + [histone H3]-L-lysine9 + 2'-O-acetyl-ADP-ribose - ? 430524 2.3.1.286 NAD+ + [histone H3]-N6-myristoyl-L-lysine9 - Homo sapiens nicotinamide + [histone H3]-L-lysine + 2'-O-myristoyl-ADP-ribose - ? 437761 2.3.1.286 NAD+ + [histone H4]-N6-acetyl-L-lysine16 - Homo sapiens nicotinamide + [histone H4]-L-lysine16 + 2'-O-acetyl-ADP-ribose - ? 429331 2.3.1.286 NAD+ + [histone H4]-N6-acetyl-L-lysine16 the deacetylation of [histone H4]-N6-acetyl-L-lysine16 may be pivotal to the formation of condensed chromatin Homo sapiens nicotinamide + [histone H4]-L-lysine16 + 2'-O-acetyl-ADP-ribose - ? 429331 2.3.1.286 NAD+ + [histone H4]-N6-acetyl-L-lysine16 strong preference for histone H4K16Ac in their deacetylation activity in vitro and in vivo Homo sapiens nicotinamide + [histone H4]-L-lysine16 + 2'-O-acetyl-ADP-ribose - ? 429331 2.3.1.286 NAD+ + [histone H4]-N6-acetyl-L-lysine8 deacetylated at high concentrations of enzyme Homo sapiens nicotinamide + [histone H4]-L-lysine8 + 2'-O-acetyl-ADP-ribose - ? 430525 2.3.1.286 NAD+ + [histone H4]-N6-myristoyl-L-lysine16 - Homo sapiens nicotinamide + [histone H4]-L-lysine + 2'-O-myristoyl-ADP-ribose - ? 437765 2.3.1.286 NAD+ + [isocitrate dehydrogenase 2]-N6-acetyl-L-lysine - Homo sapiens nicotinamide + [isocitrate dehydrogenase 2]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 429334 2.3.1.286 NAD+ + [isocitrate dehydrogenase 2]-N6-acetyl-L-lysine Sirt3 can deacetylate and activate isocitrate dehydrogenase 2, an enzyme that promotes regeneration of antioxidants and catalyzes a key regulation point of the citric acid cycle Homo sapiens nicotinamide + [isocitrate dehydrogenase 2]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 429334 2.3.1.286 NAD+ + [isocitrate dehydrogenase 2]-N6-acetyl-L-lysine413 - Mus musculus nicotinamide + [isocitrate dehydrogenase 2]-L-lysine413 + 2'-O-acetyl-ADP-ribose - ? 429333 2.3.1.286 NAD+ + [isocitrate dehydrogenase 2]-N6-acetyl-L-lysine413 acetylation of Lys413 decreases catalysis and SIRT3 reactivates isocitrate dehydrogenase 2 upon deacetylation Mus musculus nicotinamide + [isocitrate dehydrogenase 2]-L-lysine413 + 2'-O-acetyl-ADP-ribose - ? 429333 2.3.1.286 NAD+ + [nuclear receptor LXRalpha]-N6-acetyl-L-lysine432 - Homo sapiens nicotinamide + [nuclear receptor LXRalpha]-L-lysine432 + 2'-O-acetyl-ADP ribose - ? 430526 2.3.1.286 NAD+ + [nuclear receptor LXRbeta]-N6-acetyl-L-lysine433 - Homo sapiens nicotinamide + [nuclear receptor LXRbeta]-L-lysine433 + 2'-O-acetyl-ADP ribose - ? 430527 2.3.1.286 NAD+ + [nuclear receptor LXR]-N6-acetyl-L-lysine SIRT1 positively regulates liver X receptors (LXRs) by deacetylation at lysine. Deacetylation of liver X receptors (LXRs) by SIRT1 may be a mechanism that affects atherosclerosis and other agingassociated diseases Homo sapiens nicotinamide + [nuclear receptor LXR]-L-lysine + 2'-O-acetyl-ADP ribose - ? 429336 2.3.1.286 NAD+ + [p53 protein]-N6-acetyl-L-lysine - Homo sapiens nicotinamide + [p53 protein]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 429338 2.3.1.286 NAD+ + [p53 protein]-N6-acetyl-L-lysine SIRT1 deacetylase is a negative regulator of p53 function capable of modulating cellular senescence Homo sapiens nicotinamide + [p53 protein]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 429338 2.3.1.286 NAD+ + [p53 protein]-N6-acetyl-L-lysine382 - Homo sapiens nicotinamide + [p53 protein]-L-lysine382 + 2'-O-acetyl-ADP ribose - ? 429337 2.3.1.286 NAD+ + [p53 protein]-N6-acetyl-L-lysine382 Sir2 is involved in the regulation of p53 function via deacetylation Homo sapiens nicotinamide + [p53 protein]-L-lysine382 + 2'-O-acetyl-ADP ribose - ? 429337 2.3.1.286 NAD+ + [PER2 protein]-N6-acetyl-L-lysine - Homo sapiens nicotinamide + [PER2 protein]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 429339 2.3.1.286 NAD+ + [PER2 protein]-N6-acetyl-L-lysine SIRT1 regulates circadian clock gene expression through PER2 deacetylation Homo sapiens nicotinamide + [PER2 protein]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 429339 2.3.1.286 NAD+ + [protein]-N6-acetyl-L-lysine - Homo sapiens nicotinamide + [protein]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 429340 2.3.1.286 NAD+ + [protein]-N6-acyl-L-lysine - Homo sapiens nicotinamide + [protein]-L-lysine + 2'-O-acyl-ADP-ribose - ? 436528 2.3.1.286 NAD+ + [protein]-N6-acyl-L-lysine - Rattus norvegicus nicotinamide + [protein]-L-lysine + 2'-O-acyl-ADP-ribose - ? 436528 2.3.1.286 NAD+ + [protein]-N6-myristoyl-L-lysine demyristoylase activity of SIRT2 is 5fold more efficient than its deacetylase activity Homo sapiens nicotinamide + [protein]-L-lysine + 2'-O-myristoyl-ADP-ribose - ? 436529 2.3.1.286 NAD+ + [protein]-N6-succinyl-L-lysine - Homo sapiens nicotinamide + [protein]-L-lysine + 2'-O-succinyl-ADP-ribose - ? 429342 2.3.1.286 NAD+ + [SREBP-1c]-N6-acetyl-L-lysine SREBP-1c is a key lipogenic activator. Deacetylation of SREBP-1c by SIRT1 inhibits SREBP-1c activity by decreasing its stability and its association with its lipogenic target gene promoters Mus musculus nicotinamide + [SREBP-1c]-L-lysine + 2'-O-acetyl-ADP ribose - ? 429347 2.3.1.286 NAD+ + [SREBP-1c]-N6-acetyl-L-lysine SREBP-1c is a key lipogenic activator Mus musculus nicotinamide + [SREBP-1c]-L-lysine + 2'-O-acetyl-ADP ribose - ? 429347 2.3.1.286 NAD+ + [thymine DNA glycosylase]-N6-acetyl-L-lysine - Homo sapiens nicotinamide + [thymine DNA glycosylase]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 429349 2.3.1.286 NAD+ + [thymine DNA glycosylase]-N6-acetyl-L-lysine deacetylation and stimulation of the thymine DNA glycosylase activity by SIRT1 enhances DNA demethylation Homo sapiens nicotinamide + [thymine DNA glycosylase]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 429349 2.3.1.286 NAD+ + [very long-chain acyl-CoA dehydrogenase]-N6-acetyl-L-lysine - Mus musculus nicotinamide + [very long-chain acyl-CoA dehydrogenase]-L-lysine + 2'-O-acetyl-ADP-ribose - ? 436531 2.3.1.286 [Acs protein]-N6-acetyl-L-lysine609 + NAD+ + H2O - Escherichia coli [Acs protein]-L-lysine609 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446381 2.3.1.286 [Acs protein]-N6-acetyl-L-lysine609 + NAD+ + H2O the enzyme activates Acs protein by deacetylation Salmonella enterica [Acs protein]-L-lysine609 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446381 2.3.1.286 [Alba protein]-N6-acetyl-L-lysine16 + NAD+ + H2O - Saccharolobus solfataricus [Alba protein]-L-lysine16 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446383 2.3.1.286 [Alba protein]-N6-acetyl-L-lysine16 + NAD+ + H2O - Saccharolobus solfataricus P2 [Alba protein]-L-lysine16 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446383 2.3.1.286 [alpha-tubulin]-N6-acetyl-L-lysine40 + NAD+ + H2O - Homo sapiens [alpha-tubulin]-L-lysine40 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448284 2.3.1.286 [bovine serum albumin]-N6-acetyl-L-lysine + NAD+ + H2O - Archaeoglobus fulgidus [bovine serum albumin]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448287 2.3.1.286 [histone H2B]-N6-acetyl-L-lysine + NAD+ + H2O - Saccharomyces cerevisiae [histone H2B]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448298 2.3.1.286 [histone H2B]-N6-acetyl-L-lysine20 + NAD+ + H2O - Drosophila melanogaster [histone H2B]-L-lysine20 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448299 2.3.1.286 [histone H2B]-N6-acetyl-L-lysine5 + NAD+ + H2O - Drosophila melanogaster [histone H2B]-L-lysine5 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448300 2.3.1.286 [histone H2]-N6-acetyl-L-lysine + NAD+ + H2O - Saccharomyces cerevisiae [histone H2]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448301 2.3.1.286 [histone H3]-N6-acetyl-L-lysine + NAD+ + H2O - Homo sapiens [histone H3]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448302 2.3.1.286 [histone H3]-N6-acetyl-L-lysine + NAD+ + H2O - Saccharomyces cerevisiae [histone H3]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448302 2.3.1.286 [histone H3]-N6-acetyl-L-lysine + NAD+ + H2O histone H3 with the sequence ARTKQTAR(K-Ac)STGG(K-Ac)APRKQLAS. The enzyme deacetylates K9 and K16 of diacetylated histone H3 Plasmodium falciparum [histone H3]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448302 2.3.1.286 [histone H3]-N6-acetyl-L-lysine14 + NAD+ + H2O - Drosophila melanogaster [histone H3]-L-lysine14 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448304 2.3.1.286 [histone H3]-N6-acetyl-L-lysine18 + NAD+ + H2O - Drosophila melanogaster [histone H3]-L-lysine18 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448305 2.3.1.286 [histone H3]-N6-acetyl-L-lysine23 + NAD+ + H2O - Drosophila melanogaster [histone H3]-L-lysine23 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448306 2.3.1.286 [histone H3]-N6-acetyl-L-lysine9 + NAD+ + H2O - Drosophila melanogaster [histone H3]-L-lysine9 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448307 2.3.1.286 [histone H3]-N6-acetyl-L-lysine9 + NAD+ + H2O overall reaction Schizosaccharomyces pombe [histone H3]-L-lysine9 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448307 2.3.1.286 [histone H3]-N6-acetyl-L-lysine9 + NAD+ + H2O least efficient substrate Plasmodium falciparum [histone H3]-L-lysine9 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448307 2.3.1.286 [histone H3]-N6-butyryl-L-lysine9 + NAD+ + H2O - Plasmodium falciparum [histone H3]-L-lysine9 + 2''-O-butyryl-ADP-D-ribose + nicotinamide - ? 448309 2.3.1.286 [histone H3]-N6-myristoyl-L-lysine9 + NAD+ + H2O best substrate Plasmodium falciparum [histone H3]-L-lysine9 + 2''-O-myristoyl-ADP-D-ribose + nicotinamide - ? 448310 2.3.1.286 [histone H3]-N6-octanoyl-L-lysine9 + NAD+ + H2O - Plasmodium falciparum [histone H3]-L-lysine9 + 2''-O-octanoyl-ADP-D-ribose + nicotinamide - ? 448311 2.3.1.286 [histone H4]-N6-acetyl-L-lysine + NAD+ + H2O - Saccharomyces cerevisiae [histone H4]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448312 2.3.1.286 [histone H4]-N6-acetyl-L-lysine + NAD+ + H2O histone H3 with the sequence SGRG(K-Ac)GG(K-Ac)GLG(K-Ac)GGA(K-Ac)RHR. The enzyme predominantly deacetylates at K16 of tetra-acetylated histone H4 Plasmodium falciparum [histone H4]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448312 2.3.1.286 [histone H4]-N6-acetyl-L-lysine12 + NAD+ + H2O - Drosophila melanogaster [histone H4]-L-lysine12 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448314 2.3.1.286 [histone H4]-N6-acetyl-L-lysine16 + NAD+ + H2O - Saccharomyces cerevisiae [histone H4]-L-lysine16 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448315 2.3.1.286 [histone H4]-N6-acetyl-L-lysine16 + NAD+ + H2O - Drosophila melanogaster [histone H4]-L-lysine16 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448315 2.3.1.286 [histone H4]-N6-acetyl-L-lysine16 + NAD+ + H2O overall reaction Saccharomyces cerevisiae [histone H4]-L-lysine16 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448315 2.3.1.286 [histone H4]-N6-acetyl-L-lysine16 + NAD+ + H2O KGGAAc-KRHRKIL Escherichia coli [histone H4]-L-lysine16 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448315 2.3.1.286 [histone H4]-N6-acetyl-L-lysine16 + NAD+ + H2O overall reaction Saccharomyces cerevisiae BY4741 [histone H4]-L-lysine16 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448315 2.3.1.286 [histone H4]-N6-acetyl-L-lysine5 + NAD+ + H2O - Drosophila melanogaster [histone H4]-L-lysine5 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448316 2.3.1.286 [histone H4]-N6-acetyl-L-lysine8 + NAD+ + H2O weak activity Drosophila melanogaster [histone H4]-L-lysine8 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448317 2.3.1.286 [HU protein]-N6-acetyl-L-lysine + NAD+ + H2O overall reaction Mycobacterium tuberculosis [HU protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448319 2.3.1.286 [p53 peptide]-N6-acetyl-L-lysine382 + NAD+ + H2O - Escherichia coli [p53 peptide]-L-lysine382 + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448326 2.3.1.286 [peptide p300]-N6-acetyl-L-lysine + NAD+ + H2O peptide p300 with the sequence ERSTEL(K-Ac)TEI(K-Ac)EEEDQPSTS. The enzyme predominantly deacetylates at K1040 of full length peptide p300 Plasmodium falciparum [histone H4]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 448330 2.3.1.286 [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - Mus musculus [protein]-N6-acetyl-L-lysine + NAD+ + H2O - r 446429 2.3.1.286 [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - Saccharomyces cerevisiae [protein]-N6-acetyl-L-lysine + NAD+ + H2O - r 446429 2.3.1.286 [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - Archaeoglobus fulgidus [protein]-N6-acetyl-L-lysine + NAD+ + H2O - r 446429 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ - Mus musculus [protein]-N6-[1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl]-L-lysine + nicotinamide - ? 446432 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ - Saccharomyces cerevisiae [protein]-N6-[1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl]-L-lysine + nicotinamide - ? 446432 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ - Archaeoglobus fulgidus [protein]-N6-[1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl]-L-lysine + nicotinamide - ? 446432 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ - Thermotoga maritima [protein]-N6-[1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl]-L-lysine + nicotinamide - ? 446432 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ - Thermotoga maritima ATCC 43589 [protein]-N6-[1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl]-L-lysine + nicotinamide - ? 446432 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ + H2O - Saccharomyces cerevisiae [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446430 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ + H2O - Archaeoglobus fulgidus [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446430 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ + H2O overall reaction Drosophila melanogaster [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446430 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ + H2O overall reaction Mus musculus [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - r 446430 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ + H2O overall reaction Homo sapiens [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446430 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ + H2O overall reaction Saccharomyces cerevisiae [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446430 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ + H2O overall reaction Saccharomyces cerevisiae [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - r 446430 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ + H2O overall reaction Archaeoglobus fulgidus [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446430 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ + H2O overall reaction Archaeoglobus fulgidus [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - r 446430 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ + H2O overall reaction Thermotoga maritima [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446430 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ + H2O - Saccharomyces cerevisiae BY4741 [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446430 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ + H2O overall reaction Archaeoglobus fulgidus ATCC 49558 [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446430 2.3.1.286 [protein]-N6-acetyl-L-lysine + NAD+ + H2O overall reaction Thermotoga maritima ATCC 43589 [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide - ? 446430 2.3.1.286 [protein]-N6-[1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl]-L-lysine + H2O - Mus musculus [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose - ? 446433 2.3.1.286 [protein]-N6-[1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl]-L-lysine + H2O - Saccharomyces cerevisiae [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose - ? 446433 2.3.1.286 [protein]-N6-[1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl]-L-lysine + H2O - Archaeoglobus fulgidus [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose - ? 446433 2.3.1.286 [protein]-N6-[1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl]-L-lysine + H2O - Thermotoga maritima [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose - ? 446433 2.3.1.286 [protein]-N6-[1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl]-L-lysine + H2O - Thermotoga maritima ATCC 43589 [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose - ? 446433