2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wg]-L-serine - Drosophila melanogaster CoA + [Wg]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 433016 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wg]-L-serine Wnt substrate from Drosophila melanogaster Mus musculus CoA + [Wg]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 433016 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine - Gallus gallus CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432542 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine - Homo sapiens CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432542 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine - Mus musculus CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432542 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine chick WNT1 protein, the enzyme is an O-acyl transferase for WNT1 protein, WNT1 residues 214-234 are sufficient for PORCN-dependent palmitoylation of Ser224. Substitution of Ser224 with Thr, but not Cys, is tolerated in palmitoylation and biological assays. Transiently transfection of HEK-293T cells with WNT1. WNT1 mutant S224T is accepted as substrate, while mutants S224A and S224C are inactive Homo sapiens CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432542 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine chicken substrate, and chimeric recombinant substrate containing amino acids 1-63 from mouse Wnt1 (-EPSLQL) and amino acids 64-370 from chicken Wnt1 (LSRKQ-) Gallus gallus CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432542 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine chicken substrate, and chimeric recombinant substrate containing amino acids 1-63 from mouse Wnt1 (-EPSLQL) and amino acids 64-370 from chicken Wnt1 (LSRKQ-) Homo sapiens CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432542 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine chicken substrate, and chimeric recombinant substrate containing amino acids 1-63 from mouse Wnt1 (-EPSLQL) and amino acids 64-370 from chicken Wnt1 (LSRKQ-) Mus musculus CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432542 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine - Gallus gallus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine - Homo sapiens CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine - Mus musculus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine the enzyme catalyzes the palmitoylation of Ser209 and Cys77 of Wnt3a Drosophila melanogaster CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine the enzyme catalyzes the palmitoylation of Ser209 and Cys77 of Wnt3a Mus musculus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine the enzyme catalyzes the palmitoylation of Ser209 and Cys77 of Wnt3a expressed in L cells Mus musculus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a Homo sapiens CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a Mus musculus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine Wnt3a is palmitoylated in the endoplasmic reticulum and targeted to exosomes, palmitoylated Wnt3a proteins are found throughout the cyto­sol and at the plasma membrane but not in the nucleus, overview Homo sapiens CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine Wnt3a is palmitoylated in the endoplasmic reticulum and targeted to exosomes, palmitoylated Wnt3a proteins are found throughout the cyto­sol and at the plasma membrane but not in the nucleus, overview Mus musculus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine chicken substrate, and substrate from Mus musculus Gallus gallus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine chicken substrate, and substrate from Mus musculus Homo sapiens CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine chicken substrate, and substrate from Mus musculus Mus musculus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine Wnt3a is palmitoylated by fatty acids 13-16 carbons in length at Ser209 but not at Cys77, consistent with a slow turnover rate, glycosylation is not required for Wnt3a palmitoylation, which is necessary but not sufficient for Wnt3a secretion Homo sapiens CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine Wnt3a is palmitoylated by fatty acids 13-16 carbons in length at Ser209 but not at Cys77, consistent with a slow turnover rate, glycosylation is not required for Wnt3a palmitoylation, which is necessary but not sufficient for Wnt3a secretion Mus musculus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432543 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3]-L-serine - Drosophila melanogaster CoA + [Wnt3]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432544 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt5a]-L-serine - Homo sapiens CoA + [Wnt5a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432545 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt5a]-L-serine palmitoylation of Wnt-5a at Cys104 Homo sapiens CoA + [Wnt5a]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432545 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt5]-L-serine - Drosophila melanogaster CoA + [Wnt5]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432546 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg Drosophila melanogaster CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432547 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg. The enzyme binds the N-terminal 24-amino acid domain (residues 83-106) of Wg Drosophila melanogaster CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432547 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine - Homo sapiens CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432548 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine - Mus musculus CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432548 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine - Drosophila melanogaster CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432548 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine - Xenopus laevis CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432548 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine - Caenorhabditis elegans CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432548 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine the enzyme catalyzes posttranslational modification of Wnts with palmitoleic acid. PORCN is necessary and sufficient for Wnt acylation. PORCN intimately recognizes the local structure of Wnt around the site of acylation Homo sapiens CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432548 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine the enzyme is an essential mediators of Wnt secretion and signaling Mus musculus CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432548 2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine there is an increase in the catalytic activity from hexanoyl-CoA to decanoyl-CoA, after which there is an abrupt drop for dodecanoyl-CoA. Upon further increase in fatty acyl chain length, the activity stays at approximately the same level until the unsaturated palmitoleoyl-CoA, which is the best substrate Homo sapiens CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine - ? 432548 2.3.1.250 decanoyl-CoA + [Wnt]-L-serine about 30% of the activity as compared to (9Z)-hexadec-9-enoyl-CoA. There is an increase in the catalytic activity from hexanoyl-CoA to decanoyl-CoA, after which there is an abrupt drop for dodecanoyl-CoA. Upon further increase in fatty acyl chain length, the activity stays at approximately the same level until the unsaturated palmitoleoyl-CoA, which is the best substrate Homo sapiens CoA + [Wnt]-O-decanoyl-L-serine - ? 455575 2.3.1.250 dodecanoyl-CoA + [Wnt]-L-serine about 15% of the activity as compared to (9Z)-hexadec-9-enoyl-CoA. There is an increase in the catalytic activity from hexanoyl-CoA to decanoyl-CoA, after which there is an abrupt drop for dodecanoyl-CoA. Upon further increase in fatty acyl chain length, the activity stays at approximately the same level until the unsaturated palmitoleoyl-CoA, which is the best substrate Homo sapiens CoA + [Wnt]-O-dodecanoyl-L-serine - ? 455592 2.3.1.250 hexanoyl-CoA + [Wnt]-L-serine about 20% of the activity as compared to (9Z)-hexadec-9-enoyl-CoA. There is an increase in the catalytic activity from hexanoyl-CoA to decanoyl-CoA, after which there is an abrupt drop for dodecanoyl-CoA. Upon further increase in fatty acyl chain length, the activity stays at approximately the same level until the unsaturated palmitoleoyl-CoA, which is the best substrate Homo sapiens CoA + [Wnt]-O-hexanoyl-L-serine - ? 455771 2.3.1.250 additional information enzyme Porc functions on the N-terminal 24-amino acid domain (83-106) of Wg, binding of Porc with Wg is essential for its activity Drosophila melanogaster ? - ? 89 2.3.1.250 additional information PORCN splice variants differ globally in signaling activity Homo sapiens ? - ? 89 2.3.1.250 additional information generation of a fusion protein that is palmitoylated in a PORCN-dependent manner, overview Homo sapiens ? - ? 89 2.3.1.250 additional information no activity with palmitoylation-defective Wnt3aS209A mutant Homo sapiens ? - ? 89 2.3.1.250 additional information no activity with palmitoylation-defective Wnt3aS209A mutant Mus musculus ? - ? 89 2.3.1.250 additional information recombinant c-Myc-tagged substrate Wg mutants T51A, S105A, S110A, and T416A Drosophila melanogaster ? - ? 89 2.3.1.250 additional information vertebrate Wnt1 and Wnt3a possess at least one additional site for porcupine-mediated lipid-modification Homo sapiens ? - ? 89 2.3.1.250 additional information vertebrate Wnt1 and Wnt3a possess at least one additional site for porcupine-mediated lipid-modification, localization of Wnt proteins in the chick neural tube, overview Gallus gallus ? - ? 89 2.3.1.250 octanoyl-CoA + [Wnt]-L-serine about 25% of the activity as compared to (9Z)-hexadec-9-enoyl-CoA. There is an increase in the catalytic activity from hexanoyl-CoA to decanoyl-CoA, after which there is an abrupt drop for dodecanoyl-CoA. Upon further increase in fatty acyl chain length, the activity stays at approximately the same level until the unsaturated palmitoleoyl-CoA, which is the best substrate Homo sapiens CoA + [Wnt]-O-octanoyl-L-serine - ? 456121 2.3.1.250 tetradecanoyl-CoA + [Wnt]-L-serine about 10% of the activity as compared to (9Z)-hexadec-9-enoyl-CoA. There is an increase in the catalytic activity from hexanoyl-CoA to decanoyl-CoA, after which there is an abrupt drop for dodecanoyl-CoA. Upon further increase in fatty acyl chain length, the activity stays at approximately the same level until the unsaturated palmitoleoyl-CoA, which is the best substrate Homo sapiens CoA + [Wnt]-O-tetradecanoyl-L-serine - ? 456824 2.3.1.250 [125I]iodo-pentadecenoyl-CoA + [Wnt]-L-serine [125I]iodo-pentadecenoyl-CoA is a radioiodinated palmitoleate analog Mus musculus CoA + [Wnt]-O-[125I]iodo-pentadecenoyl-L-serine - ? 456956