2.3.1.158 1,2-dioleoylphosphatidylcholine + 1,2-diacylglycerol - Saccharomyces cerevisiae 1-oleoyl-2-lysophosphatidylcholine + triacylglycerol - ? 259605 2.3.1.158 1,2-dioleoylphosphatidylcholine + 1,2-diacylglycerol - Arabidopsis thaliana 1-oleoyl-2-lysophosphatidylcholine + triacylglycerol - ? 259605 2.3.1.158 1,2-dioleoylphosphatidylcholine + 1,2-diacylglycerol - Ricinus communis 1-oleoyl-2-lysophosphatidylcholine + triacylglycerol - ? 259605 2.3.1.158 1,2-dioleoylphosphatidylcholine + 1,2-diacylglycerol - Helianthus annuus 1-oleoyl-2-lysophosphatidylcholine + triacylglycerol - ? 259605 2.3.1.158 1,2-dioleoylphosphatidylcholine + 1,2-diacylglycerol - Crepis palaestina 1-oleoyl-2-lysophosphatidylcholine + triacylglycerol - ? 259605 2.3.1.158 1,2-dioleoylphosphatidylethanolamine + 1,2-dioleoylglycerol - Saccharomyces cerevisiae 1-oleoyl-2-lysophosphatidylethanolamine + trioleoylglycerol - ? 259608 2.3.1.158 acyl-CoA + 1,2-diacyl-sn-glycerol - Brassica napus CoA + 1,2,3-triacylglycerol - ? 454309 2.3.1.158 acyl-CoA + 1,2-diacyl-sn-glycerol - Crepis palaestina CoA + 1,2,3-triacylglycerol - ? 454309 2.3.1.158 acyl-CoA + 1,2-diacyl-sn-glycerol - Arabidopsis thaliana CoA + 1,2,3-triacylglycerol - ? 454309 2.3.1.158 acyl-CoA + 1,2-diacyl-sn-glycerol - Saccharomyces cerevisiae CoA + 1,2,3-triacylglycerol - ? 454309 2.3.1.158 acyl-CoA + 1,2-diacyl-sn-glycerol - Helianthus annuus CoA + 1,2,3-triacylglycerol - ? 454309 2.3.1.158 acyl-CoA + 1,2-diacyl-sn-glycerol - Ricinus communis CoA + 1,2,3-triacylglycerol - ? 454309 2.3.1.158 acyl-CoA + 1,2-diacyl-sn-glycerol - Saccharomyces cerevisiae ATCC 204508 CoA + 1,2,3-triacylglycerol - ? 454309 2.3.1.158 dioleoyl-phosphatidylcholine + 1,2-diacylglycerol - Saccharomyces cerevisiae lyso-phosphatidylcholine + triacylglycerol - ? 259606 2.3.1.158 dioleoylphosphatidylcholine + 1,2-dioleoylglycerol - Saccharomyces cerevisiae 1-oleoyl-2-lyso-phosphatidylcholine + trioleoylglycerol - ? 259607 2.3.1.158 dioleoylphosphatidylcholine + 1-ricinoleoylglycerol - Saccharomyces cerevisiae 1-oleoyl-2-lysophosphatidylcholine + 1-ricinoleoyl-2-oleoyl-glycerol - ? 259609 2.3.1.158 dioleoylphosphatidylcholine + 1-vernoloylglycerol - Saccharomyces cerevisiae 1-oleoyl-2-lysophosphatidylcholine + 1-vernoloyl-2-oleoylglycerol - ? 259610 2.3.1.158 linolenoyl-phosphatidylcholine + 1,2-dilinolenoylglycerol - Linum usitatissimum lyso-phosphatidylcholine + trilinolenoylglycerol - ? 435593 2.3.1.158 additional information PDAT shows broad substrate specificity, overview Chlamydomonas reinhardtii ? - ? 89 2.3.1.158 additional information in the presence of alpha-linolenic acid, isoform PDAT1 produces triacylglycerol with a specific fatty acid composition. PDAT1 has the ability to synthesize trilinolenin, which is the major molecular species of triacylglycerol in flax oil Linum usitatissimum ? - ? 89 2.3.1.158 additional information in the presence of alpha-linolenic acid, isoform PDAT2 produces triacylglycerol with a specific fatty acid composition. PDAT2 has the ability to synthesize trilinolenin, which is the major molecular species of triacylglycerol in flax oil Linum usitatissimum ? - ? 89 2.3.1.158 additional information Saccharoymces cerevisiae PDAT also displays low DAG:DAG transacylase activity Saccharomyces cerevisiae ? - - 89 2.3.1.158 additional information Saccharoymces cerevisiae PDAT also displays low DAG:DAG transacylase activity Saccharomyces cerevisiae ATCC 204508 ? - - 89 2.3.1.158 palmitoyl phospholipid + dioleoylglycerol 70% less active in mutants lacking the acyl-CoA-independent acyltransferase activity, individual neutral lipids affects the internal structure of lipid particles Saccharomyces cerevisiae 1-lysophospholipid + 1-palmitoyl-2,3-dioleoyl-sn-glycerol - ? 398059 2.3.1.158 phosphatidylcholine + 1,2-diacylglycerol lysophophatidylcholine acyltransferase activity is mainly responsible for the entry of oleate and linoleate into olive callus lipid metabolism, PDAT is involved in triacylglyceride biosynthesis Olea europaea 2-lysophosphatidylcholine + triacylglycerol - ? 398117 2.3.1.158 phosphatidylethanolamine + 1,2-diacylglycerol - Streptomyces coelicolor 2-lysophosphatidylethanolamine + triacylglycerol - ? 398121 2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol - Ricinus communis lysophospholipid + triacylglycerol - ? 419530 2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol - Lobosphaera incisa lysophospholipid + triacylglycerol - ? 419530 2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol - Sesamum indicum lysophospholipid + triacylglycerol - ? 419530 2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol PDAT uses membrane lipids (e.g., phospholipids and glycolipids) as the substrates for the acyl transfer reaction or hydrolysis in vivo Chlamydomonas reinhardtii lysophospholipid + triacylglycerol - ? 419530 2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol the enzyme is capable of using broad acyl donors such as PA, PS, PG, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, and acyl-CoA. The enzyme is more likely to use unsaturated acyl donors comparing 18:0/18:1 to 18:0/18:0 phospholipids. With regard to acyl acceptors, the enzyme prefers 1,2 to 1,3-diacylglycerol, while 12:0/12:0 1,3-diacylglycerol is identified as the optimal acyl acceptor, followed by 18:1/18:1 and 18:1/16:0 1,3-diacylglycerol Saccharomyces cerevisiae lysophospholipid + triacylglycerol - ? 419530 2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol the enzyme is capable of using broad acyl donors such as PA, PS, PG, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, and acyl-CoA. The enzyme is more likely to use unsaturated acyl donors comparing 18:0/18:1 to 18:0/18:0 phospholipids. With regard to acyl acceptors, the enzyme prefers 1,2 to 1,3-diacylglycerol, while 12:0/12:0 1,3-diacylglycerol is identified as the optimal acyl acceptor, followed by 18:1/18:1 and 18:1/16:0 1,3-diacylglycerol Saccharomyces cerevisiae ATCC 204508 lysophospholipid + triacylglycerol - ? 419530 2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol - Lobosphaera incisa Reisigl H4301 lysophospholipid + triacylglycerol - ? 419530 2.3.1.158 phospholipid + 1,2-diacylglycerol - Saccharomyces cerevisiae lysophospholipid + triacylglycerol - ? 370186 2.3.1.158 phospholipid + 1,2-diacylglycerol - Arabidopsis thaliana lysophospholipid + triacylglycerol - ? 370186 2.3.1.158 phospholipid + 1,2-diacylglycerol - Ricinus communis lysophospholipid + triacylglycerol - ? 370186 2.3.1.158 phospholipid + 1,2-diacylglycerol - Helianthus annuus lysophospholipid + triacylglycerol - ? 370186 2.3.1.158 phospholipid + 1,2-diacylglycerol - Crepis palaestina lysophospholipid + triacylglycerol - ? 370186 2.3.1.158 phospholipid + 1,2-diacylglycerol - Euphorbia lagascae lysophospholipid + triacylglycerol - ? 370186 2.3.1.158 phospholipid + 1,2-diacylglycerol the enzyme plays a major role in removing ricinoleic acid and vernolic acid from phospholipids in seeds Ricinus communis lysophospholipid + triacylglycerol - ? 370186 2.3.1.158 phospholipid + 1,2-diacylglycerol the enzyme plays a major role in removing ricinoleic acid and vernolic acid from phospholipids in seeds Crepis palaestina lysophospholipid + triacylglycerol - ? 370186 2.3.1.158 phospholipid + 1,2-diacylglycerol enzyme accepts acyl groups ranging from C10 to C22, activity is highly dependent on the acyl composition preferring acyl groups with several double bonds, epoxy, or hydroxy groups, enzyme has a 3fold preference for the sn-2 compared to sn-1 position of phosphatidylcholine, overview Arabidopsis thaliana lysophospholipid + triacylglycerol - ? 370186 2.3.1.158 phospholipid + 1,2-dipalmitoyl-sn-glycerol levels of triacylglyceride biosynthesis through the PDAT pathway were comparable in wild type and mutants, lacking of the acyl-coenzyme A: DAG acyltransferase activity has no effect on the PDAT activity, this is the first time that a PDAT activity has been reported for bacteria Streptomyces coelicolor lysophospholipid + 3-acyl-1,2-dipalmitoyl-sn-glycerol - ? 398126 2.3.1.158 rac-1,2-divernoleoylglycerol + 1,3-dioleoyl-sn-glycerol - Saccharomyces cerevisiae 1-oleoyl-sn-glycerol + rac-1,2-divernoleoyl-3-oleoylglycerol - ? 398239 2.3.1.158 ricinoleoyl-phosphatidylcholine + 1,2-diacylglycerol - Saccharomyces cerevisiae lyso-phosphatidylcholine + triacylglycerol - ? 259604 2.3.1.158 ricinoleoyl-phosphatidylcholine + 1,2-diacylglycerol - Arabidopsis thaliana lyso-phosphatidylcholine + triacylglycerol - ? 259604 2.3.1.158 ricinoleoyl-phosphatidylcholine + 1,2-diacylglycerol - Ricinus communis lyso-phosphatidylcholine + triacylglycerol - ? 259604 2.3.1.158 ricinoleoyl-phosphatidylcholine + 1,2-diacylglycerol - Helianthus annuus lyso-phosphatidylcholine + triacylglycerol - ? 259604 2.3.1.158 ricinoleoyl-phosphatidylcholine + 1,2-diacylglycerol - Crepis palaestina lyso-phosphatidylcholine + triacylglycerol - ? 259604 2.3.1.158 ricinoleoyl-phosphatidylcholine + 1,2-diacylglycerol - Euphorbia lagascae lyso-phosphatidylcholine + triacylglycerol - ? 259604 2.3.1.158 sn-1,2-dioleoylglycerol + sn-1,2-dioleoylglycerol - Saccharomyces cerevisiae trioleoylglycerol + sn-1-oleoylglycerol - ? 398323 2.3.1.158 sn-1,2-dioleoylphosphatidylcholine + sn-1,2-dioleoylglycerol - Saccharomyces cerevisiae trioleoylglycerol + sn-1-oleoylphosphatidylcholine - ? 398324 2.3.1.158 sn-1,2-dioleoylphosphatidylcholine + sn-1-oleoylglycerol - Saccharomyces cerevisiae ? + sn-1-oleoylphosphatidylcholine - ? 398325 2.3.1.158 sn-1,2-dioleoylphosphatidylcholine + sn-2-oleoylglycerol - Saccharomyces cerevisiae ? + sn-1-oleoylphosphatidylcholine - ? 398326 2.3.1.158 sn-1,2-dioleoylphosphatidylethanolamine + sn-1,2-dioleoylglycerol - Saccharomyces cerevisiae trioleoylglycerol + 1-oleoylphosphatidylethanolamine - ? 398327 2.3.1.158 sn-1,3-dioleoylglycerol + sn-1,3-dioleoylglycerol - Saccharomyces cerevisiae trioleoylglycerol + sn-1-oleoylglycerol - ? 398328 2.3.1.158 sn-2-acyl-phosphatidylcholine + 1,2-diacylglycerol - Arabidopsis thaliana triacylglycerol + glycerophosphocholine - ? 259602 2.3.1.158 sn-2-acyl-phosphatidylcholine + 1,2-diacylglycerol - Ricinus communis triacylglycerol + glycerophosphocholine - ? 259602 2.3.1.158 sn-2-acyl-phosphatidylcholine + 1,2-diacylglycerol - Helianthus annuus triacylglycerol + glycerophosphocholine - ? 259602 2.3.1.158 sn-2-acyl-phosphatidylcholine + 1,2-diacylglycerol - Crepis palaestina triacylglycerol + glycerophosphocholine - ? 259602 2.3.1.158 sn-2-acyl-phosphatidylcholine + 1,2-diacylglycerol - Euphorbia lagascae triacylglycerol + glycerophosphocholine - ? 259602 2.3.1.158 vernoloyl-phosphatidylcholine + 1,2-diacylglycerol - Saccharomyces cerevisiae lyso-phosphatidylcholine + triacylglycerol - ? 259603 2.3.1.158 vernoloyl-phosphatidylcholine + 1,2-diacylglycerol - Ricinus communis lyso-phosphatidylcholine + triacylglycerol - ? 259603 2.3.1.158 vernoloyl-phosphatidylcholine + 1,2-diacylglycerol - Helianthus annuus lyso-phosphatidylcholine + triacylglycerol - ? 259603 2.3.1.158 vernoloyl-phosphatidylcholine + 1,2-diacylglycerol - Crepis palaestina lyso-phosphatidylcholine + triacylglycerol - ? 259603 2.3.1.158 vernoloyl-phosphatidylcholine + 1,2-diacylglycerol - Euphorbia lagascae lyso-phosphatidylcholine + triacylglycerol - ? 259603