2.1.1.72 additional information S-adenosyl-L-methionine plays a crucial role in reorientation of the enzyme in DNA with mutationally altered Ade residues to 2-aminopurine, overview Tequatrovirus T4 ? - ? 89 2.1.1.72 additional information DNA substrate specificities of wild-type and mutant enzymes Tequatrovirus T4 ? - ? 89 2.1.1.72 additional information enzyme and S-adenosyl-L-methionine form a catalytically active complex Tequatrovirus T4 ? - ? 89 2.1.1.72 additional information enzyme binds also nonspecific to DNA, linear diffusion along the DNA, overview Tequatrovirus T4 ? - ? 89 2.1.1.72 additional information substrate specificity with different oligonucleotides, substrate binding specificity, overview Escherichia coli ? - ? 89 2.1.1.72 additional information the common methyl transfer from S-adenosyl-L-methionine to an exocyclic amino group, catalyzed by many enzymes, does not dictate a common kinetic scheme for MTases, comparison to T4Dam MTase from bacteriophage T4 Bacillus amyloliquefaciens ? - ? 89 2.1.1.72 additional information catalyzes the transfer of a methyl group to the C5 position of the 3'-side cytosine of each strand of the recognition sequence, M.EcoRII binding is diminished by factors of 5-30 but the catalytic activity is either abolished or reduced 4-80fold when trans-anti-B[a]P-N2-dG lesions are introduced into the EcoRII recognition sequence, methylation rates are also diminished and in some cases entirely abolished, depending on the position of the lesion within the recognition sequence Escherichia coli ? - ? 89 2.1.1.72 additional information DNA methylation by DAM may not globally affect gene transcription by physically blocking access of transcription factors to binding sites, Dam is down regulated in the stationary phase, which correlates with the enrichment of GATC in binding sites for CRP and Sigma 38 Escherichia coli ? - ? 89 2.1.1.72 additional information the enzyme is part of the type II restriction-modification system AhdI, overview Aeromonas hydrophila ? - ? 89 2.1.1.72 additional information Dam-dependent regulation of secretion of pathogenicity island 1 is transmitted via transcriptional regulator HilD Salmonella enterica ? - ? 89 2.1.1.72 additional information MmeI does not produce any detectable N4-cytosine methylation Methylophilus methylotrophus ? - ? 89 2.1.1.72 additional information no detectable activity on the single-stranded form of the phage PhiX174 DNA is observed with M1.MboII, double-stranded DNA is less efficiently methylated than pUC18 DNA Moraxella bovis ? - ? 89 2.1.1.72 additional information plastid transcription is largely insensitive to adenine methylation of the plastid DNA Synechocystis sp. ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Neisseria meningitidis ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Bacillus sp. (in: Bacteria) ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Deinococcus radiodurans ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Neisseria lactamica ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Synechococcus sp. PCC 7002 ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Corynebacterium striatum ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Methylophilus methylotrophus ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Trueperella pyogenes ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Corynebacterium diphtheriae ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Marinobacter nauticus ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Nitrobacter hamburgensis ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Parvibaculum lavamentivorans ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Pseudomonas sp. ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Psychrobacter sp. ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Rhodospirillum centenum ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Rhodopseudomonas palustris ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Sulfurimonas denitrificans ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Ruegeria pomeroyi ? - ? 89 2.1.1.72 additional information the preferred substrate consists of the annealed product of oligonucleotides 5'-CATTTACTTGATCCGGTATGC-3' and 5'-GCATACCGGATCAAGTAAATG-3', while the nonpreferred substrate consists of the annealed product of oligonucleotides 5'-CATTTAGACGATCTTTTATGC-3' and 5'-GCATAAAAGATCGTCTAAATG-3' Escherichia coli ? - ? 89 2.1.1.72 additional information the wild type Dam shows no detectable activity at GATT sites Escherichia coli ? - ? 89 2.1.1.72 additional information purified recombinant enzyme, in the presence of S-adenosyl-L-methionine and other cofactors, is unable to methylate trivalent inorganic arsenic or monomethylarsonous acid Homo sapiens ? - ? 89 2.1.1.72 additional information the enzyme has a high specificity for GANTC sites, with only minor preferences at the central position. It slightly (1.5fold) prefers hemimethylated DNA over methylated DNA Caulobacter vibrioides ? - ? 89 2.1.1.72 additional information the enzyme recognizes and methylates GAGG (100% activity, Km 0.00522 mM), GGAG (about 48% activity, Km 0.017 mM) and GAAG (about 30% activity, Km 0.013 mM), but does not methylate GTGG, GmAmAG or GAGA Helicobacter pylori ? - ? 89 2.1.1.72 additional information the wild type enzyme is active on hemimethylated and unmethylated DNA substrates Escherichia coli ? - ? 89 2.1.1.72 additional information relative to GATC, three near-cognate substrates that carry a base-pair substitution at the first position are still methylated by the enzyme, although at rates reduced by 100fold (AATC) or 1000fold (CATC) Escherichia coli ? - ? 89 2.1.1.72 additional information the enzyme methylates GANTC sequences but does not specifically bind to DNA containing GANTC sequences Caulobacter vibrioides ? - ? 89 2.1.1.72 additional information the enzyme methylates adenine residues in 5'-GANTC-3' sequences Caulobacter vibrioides ? - - 89 2.1.1.72 additional information the DNA substrate preference for M.EcoVIII is: hemimethylated AAGCTT > AAGCTT > GAGCTT >/= CAGCTT > AATCTT > ATGCTT > ACGCTT > TAGCTT > AACCTT = single stranded AAGCTT > AGGCTT = AAACTT Escherichia coli ? - - 89 2.1.1.72 additional information the enzyme complex shows strong activity on single-stranded oligo 6 T (containing GGACT), much reduced activities on 4 T (containing GGAGT) and 4B (AGACT), and no activity on oligos having two substitutions within the recognition, e.g. 6B (AGAGT) or 5 T and 5B (GGATC). Furthermore, the enzyme complex has no activity on an oligo-containing G and C only and no activity on A-containing oligos without matching consensus sequence. The enzyme complex acts on single-stranded DNA (6 T) faster than that on the corresponding single-stranded RNA and is inactive on double-stranded DNA or RNA/DNA hybrid Homo sapiens ? - - 89 2.1.1.72 additional information the enzyme does not specifically bind to DNA containing GANTC sequences Caulobacter vibrioides ? - - 89 2.1.1.72 additional information the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') Escherichia coli ? - - 89 2.1.1.72 additional information the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine Haemophilus influenzae ? - - 89 2.1.1.72 additional information the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine Lactococcus cremoris ? - - 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Corynebacterium striatum M82B ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Neisseria meningitidis Z2491 ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Rhodopseudomonas palustris BisB5 ? - ? 89 2.1.1.72 additional information the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine Haemophilus influenzae RD ? - - 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Deinococcus radiodurans RI ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Neisseria lactamica ST640 Sange ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Rhodospirillum centenum SW ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Deinococcus radiodurans NEB479 ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Bacillus sp. (in: Bacteria) NEB686 ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Nitrobacter hamburgensis X14 ? - ? 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Synechococcus sp. PCC 7002 PR-6 ? - ? 89 2.1.1.72 additional information the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine Lactococcus cremoris W15 ? - - 89 2.1.1.72 additional information the enzyme does not produce any detectable N4-cytosine methylation Pseudomonas sp. OM2164 ? - ? 89 2.1.1.72 additional information the DNA substrate preference for M.EcoVIII is: hemimethylated AAGCTT > AAGCTT > GAGCTT >/= CAGCTT > AATCTT > ATGCTT > ACGCTT > TAGCTT > AACCTT = single stranded AAGCTT > AGGCTT = AAACTT Escherichia coli E1585-68 ? - - 89 2.1.1.72 additional information the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') Escherichia coli E1585-68 ? - - 89 2.1.1.72 S-adenosyl-L-methionine + (CGG*CCG)73 oligodeoxynucleotide duplexes 5'-GATC/5'-GATC - Tequatrovirus T4 ? - ? 453918 2.1.1.72 S-adenosyl-L-methionine + adenine in DNA - Escherichia coli S-adenosyl-L-homocysteine + N6-methyladenine in DNA - ? 451890 2.1.1.72 S-adenosyl-L-methionine + adenine in DNA - Caenorhabditis elegans S-adenosyl-L-homocysteine + N6-methyladenine in DNA - ? 451890 2.1.1.72 S-adenosyl-L-methionine + adenine in DNA - Klebsiella pneumoniae S-adenosyl-L-homocysteine + N6-methyladenine in DNA - r 451890 2.1.1.72 S-adenosyl-L-methionine + adenine in DNA - Caulobacter vibrioides S-adenosyl-L-homocysteine + N6-methyladenine in DNA - ? 451890 2.1.1.72 S-adenosyl-L-methionine + adenine in DNA - Cellulosimicrobium cellulans S-adenosyl-L-homocysteine + N6-methyladenine in DNA - ? 451890 2.1.1.72 S-adenosyl-L-methionine + adenine in DNA - Cellulosimicrobium cellulans B S-adenosyl-L-homocysteine + N6-methyladenine in DNA - ? 451890 2.1.1.72 S-adenosyl-L-methionine + adenine in DNA the enzyme specifically binds double-stranded DNA Escherichia coli S-adenosyl-L-homocysteine + DNA containing N6-methyladenine - ? 454503 2.1.1.72 S-adenosyl-L-methionine + adenine in T4 gt- dam- DNA - Tequatrovirus T4 S-adenosyl-L-homocysteine + N6-methyladenine in T4 gt- dam- DNA - ? 451889 2.1.1.72 S-adenosyl-L-methionine + adenine in T4 gt- dam- DNA - Escherichia phage T2 S-adenosyl-L-homocysteine + N6-methyladenine in T4 gt- dam- DNA - ? 451889 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Bacteria S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Thermus aquaticus S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Bacillus amyloliquefaciens S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Helicobacter pylori S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Yersinia pseudotuberculosis S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Vibrio sp. S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Planomicrobium okeanokoites S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Escherichia phage T2 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Cereibacter sphaeroides S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme mediates methyl group transfer reaction from S-adenosyl-L-methionine to adenine in the palindromic recognition sequence, GATC, of a 20-mer oligonucleotide duplex Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine GGGTGATCAGGG Triticum aestivum S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine CCCTGATCACCC Triticum aestivum S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme modifies the third adenine within the recognition sequence 5-ATTAAT-3' Vibrio sp. S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine canonical 14mers and various substituted duplexes. Non-selfcomplementary tetradecamer duplex d[GCCGGATCTAGACG]*d[CGTCTAGATCCGGC] containing the hemimethylated GATC target sequence on one or the other strand and modifications in the GATC target sequence of the complementary strands. Large differences in DNA methylation of duplexes containing single dI or dG substitutions of the Dam recognition site are observed compared with the canonical substrate, if the substitution involves the top strand, on the G-C rich side. Substitution in either strand by uracil or 5-ethyluracil result in small perturbation of the methylation patterns. When 2,6-diamino-purine replaces the adenine to be methylated, small but significant methylation is observed Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine in unmethylated target (2A/A duplex), the enzyme alone randomly binds to the asymetric 2A/A duplex, S-adenosyl-L-methionine induces an allosteric T4 conformational change that promotes reorientation of the enzyme to the strand containing the native base. S-Adenosyl-L-methionine increases enzyme binding-specificity, in addition to serving as the methyl donor, the enzyme recognizes the palindromic sequence GATC Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine various synthetic oligonucleotide substrates. Upon collision of an enzyme monomer with an oligonucleotide duplex, an asymmetrical complex forms in which the enzyme (randomly oriented relative to one of the strands of the specific recognition site) catalyzes a fast transfer of the methyl group from S-adenmosylmethionine to the adenosine residue. Simultaneously, a second T4MTAse subunit is added to the complex, providing for the continuation of the reaction Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme catalyzes methyl group transfer from S-adenosyl-L-methionine to the N6-position of adenine in the palindromic sequence GATC. The rate-limiting step is release of product methylated DNA from the enzyme Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine oligonucleotide substrates containing the native or modified recognition site. The enzyme recognizes the palindromic sequence GATC and catalyzes transfer of the methyl group from S-adenosyl-L-methionine to the N6-position of adenine. The release of product is the rate-limiting step in the reaction Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme preferentially binds DNA before S-adenosylmethionine. S-Adenosylhomocysteine is preferentially released from the enzyme before fully methylated DNA. Binding of both substrates and methylation occurs first in a rapid step followed by regeneration of enzyme in a second rate-limiting step Caulobacter vibrioides S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme de novo methylates the first adenine residue in the TGATCA sequence in the single-stranded or double-stranded DNA substrate, but it prefers single-stranded structures Triticum aestivum S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine canonical 12-mer and 20-mers various defective duplexes containing some defect in the DNA-target site, e.g. the absence of an internucleotide phosphate or a nucleotide within the recognition site, or a single-stranded region Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine non-self-complementary tetradecanucleotide duplexes that contain the GATC target sequence. The enzyme is rather tolerant to base modification, binding of the enzyme is inversely proportional to the thermodynamic stability of the duplex in the ternary complex Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine GCGTGATCACGC Triticum aestivum S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine nonglucosylated unmethylated T4 gt- dam- DNA. The enzyme methylates the palindromic tetranucleotide, GATC, designated the canonical sequence. At high Mtase:DNA ratios, T4 Dam can methylate some noncanonical sequences belonging to GAY, where Y represents cytosine or thymine Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine GGGTGATCAGGG+CCCTGATCACCC, annealed Triticum aestivum S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine synthetic oligonucleotide substrates containing the native recognition site GATC or its modified variants Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine interaction with substrates containing defective recognition sites. Deoxyguanosine residues in both strands of the modified GATC are indispensable for complex formation Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine nonglucosylated, hydroxymethylcytosine-containing T2gt- virion DNA has a higher level of methylation than T4gt virion DNA does Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine nonglucosylated, hydroxymethylcytosine-containing T2gt- virion DNA has a higher level of methylation than T4gt virion DNA does Escherichia phage T2 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine lambda phage DNA Triticum aestivum S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine methylation of DNA in a sequence specific manner, low substrate specificity with respect to the target base. Cytosine residues can be methylated if they are located in a C:T mismatch base pair at the target position of the enzyme, modification of cytosine residues at position N4 Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme is responsible for mitochondrial DNA modification that might be involved in the regulation of replication of mitochondria in plants Triticum aestivum S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme is involved in cell cycle regulation of Caulobacter crescentus Caulobacter vibrioides S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine enzyme is a critical regulator of bacterial virulence Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine enzyme is a member of a restriction-modification system, R-M system, plasmid DNA, and hemi- or unmethylated duplex DNA, preference for hemimethylated DNA Klebsiella pneumoniae S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine enzyme is not important in mismatch repair and for adherence of the bacterium to host cells, e.g. HEp-2 cells Helicobacter pylori S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine hemimethylated DNA Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine methylation of DNA-adenine at certain GATC sites plays a pivotal role in bacterial and phage gene expression as well as in bacterial virulence Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine methylation of the site GATCprox proximal to the promotor is required for transition to the phase On state by specifically blocking PapI-dependent binding of Lrp to promotor proximal sites 4-6, expression of pyelonephritis-associated pili, i.e. Pap, in uropathogenic Escherichia coli is epigenetically controlled by a reversible OFF to ON switch, overview Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine specific target sequence is GATC Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme is cell cycle regulated and essential for viability Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine unmethylated or hemimethylated DNA Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine binding site is the GATC sequence, the adenine is located in the palindromic recognition site GATC, substrate binding mechanism Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine DNA substrate from calf thymus, methylation of adjacent GATC sites is distributive with DNA derived from a genetic element that controls the transcription of the adjacent genes, the first methylation event is followed by enzyme release Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine enzyme methylates the first adenine in the sequence ATGCAT Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine enzyme recognizes the sequence 5'-GGTACC-3', methylation at position N6, enzyme utilizes plasmid DNA, and hemi- or unmethylated duplex DNA, preference for hemimethylated DNA Klebsiella pneumoniae S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine methylation of target sequence GATC Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine required substrate recognition target sequence is GATC, occuring base flipping in absence of S-adenosyl-L-methionine is a biphasic process and very fast, but binding of the flipped base into the active site pocket requiring S-adenosyl-L-methionine is slow, active site contains the conserved DPPY motif, whose tyrosine184 residue stacks to the flipped target base Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine specific for methylation site sequence GATC and structure, an intact GA sequence is essential for activity, altered structural symmetry of the DNA substrate decreases kcat sharply, the best contact between enzyme and DNA is a palindromic interaction site covering the 5'-symmetric residues, which is located in the major groove, and another one in the 3'-half covering the 3'-symmetric residues is located in the minor groove, overview Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine specific for methylation site sequence GATC and structure, an intact GAT sequence is essential for activity, altered structural symmetry of the DNA substrate decreases kcat sharply, the best contact between enzyme and DNA is a palindromic interaction site covering the 5'-symmetric residues, which is located in the major groove, and another one in the 3'-half covering the 3'-symmetric residues is located in the minor groove, overview Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine specific methylation of oligonucleotide duplexes containing one or two target sequence GATC sites Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine specific methylation of target sequence GATC Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine specific target sequence is GATC, transition of enzyme-DNA interaction from nonspecific to specific interaction utilizing different substrates, identification of discriminatory contacts stabilizing the transition state, and antidiscriminatory contacts not affecting the methylation of the cognate site but disfavor activity at noncognate sites, involved residues are M114, R116, P126, G128, R130, F111, S112, D171, K11, and Y174, overview, flipping of target adenine Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine substrate and product binding site structures Cereibacter sphaeroides S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine transition of enzyme-DNA interaction from nonspecific to specific interaction utilizing different substrates, identification of discriminatory contacts stabilizing the transition state, and antidiscriminatory contacts not affecting the methylation of the cognate site but disfavor activity at noncognate sites, involved are Y119, N120, L122, R124, and P134, overview Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine unmethylated or hemimethylated DNA, two distinct stages of methylation under single turnover conditions Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Aliivibrio fischeri S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Vibrio parahaemolyticus S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Vibrio cholerae serotype O1 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Treponema succinifaciens S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Vibrio anguillarum S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Vibrio vulnificus S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Vibrio campbellii S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Vibrio splendidus S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Deinococcus proteolyticus S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Paludibacter propionicigenes S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Vibrio phage VHML S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Hapunavirus VP882 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Vibrio cholerae S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes 5'-GATC-3' Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine a biotinylated double stranded 23mer oligodeoxynucleotide with one hemimethylated GANTC site is used as substrate Caulobacter vibrioides S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme catalyzes the methylation of adenine at the N-6 position within its DNA recognition sequence GATC. Enzymatic methylation of the hemimethylated GATC site results in destabilisation of the duplex, increasing the proportion of dissociated DNA, and producing an observable increase in fluorescence. By using a suitable oligonucleotide substrate, labeled on adjacent strands with a fluorophore (fluorescein) and quencher (dabcyl), the destabilization of duplex DNA due to adenine methylation can be monitored by fluorescence measurements. The separation of fluorophore and quencher during strand dissociation causes an observable increase in fluorescence, providing a reproducible, direct, and real-time activity assay Pyrococcus horikoshii S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Paludibacter propionicigenes WB4T S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Treponema succinifaciens DSM 2489 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Vibrio campbellii ATCC BAA-1116 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Vibrio anguillarum 775 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Vibrio vulnificus MO6-24/O S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Vibrio splendidus LGP32 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Vibrio cholerae O395 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Deinococcus proteolyticus MRP S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates GATC sequences Vibrio parahaemolyticus RIMD S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 319867 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Cereibacter sphaeroides S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Enterobacter cloacae S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Photorhabdus luminescens S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Neisseria meningitidis S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Klebsiella pneumoniae S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Bacillus sp. (in: Bacteria) S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Aeromonas hydrophila S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Salmonella enterica S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Caulobacter vibrioides S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Tetrahymena thermophila S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Deinococcus radiodurans S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Neisseria lactamica S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Synechococcus sp. PCC 7002 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Corynebacterium striatum S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Methylophilus methylotrophus S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Trueperella pyogenes S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Corynebacterium diphtheriae S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Marinobacter nauticus S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Nitrobacter hamburgensis S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Parvibaculum lavamentivorans S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Pseudomonas sp. S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Psychrobacter sp. S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Rhodospirillum centenum S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Rhodopseudomonas palustris S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Sulfurimonas denitrificans S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Ruegeria pomeroyi S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Thermus aquaticus S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Streptococcus pneumoniae S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Moraxella bovis S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine Dam belongs to the alpha-class of adenine methyltransferases and transfers a methyl group to the N-6 position of the adenine in the DNA sequence 5'-GATC-3' Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine Dam catalyzes postreplicative methylation of adenosine moieties located in 5'-GATC-3' sites Salmonella enterica S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine Dam catalyzes the methylation of N-6 of the adenine residue in GATC sequences Edwardsiella tarda S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine Dam methylates a GATC recognition site Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine Dam methylates the adenine residue in GATC sites Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine Dam methylates the N-6 position of adenine in the DNA sequence 5'-GATC-3' and is highly processive, catalyzing multiple methyltransfers prior to dissociating from the DNA Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine Dam methylates the N-6 position of the adenine in the sequence 5'-GATC-3', Dam shows a dramatic preference for the in vitro methylation of certain GATC sequences in plasmids and PCR-derived DNAfragments Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine DNA adenine methyltransferase methylates the N6 positions of adenines in the sequence 5'-GATC-3' Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine EcoP15I MTase adds a methyl group to the second adenine in the recognition sequence 5'-CAGCAG-3' in the presence of S-adenosyl-L-methionine Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine M.HpyAXII targets GTAC sites Helicobacter pylori S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine M1.MboII modifies the last adenine in the recognition sequence 5'-GAAGA-3' to N6-methyladenine Moraxella bovis S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine MmeI modifies only the adenine in the top strand, 5’-TCCRAC-3’, MmeI endonuclease activity is blocked by this top strand adenine methylation and is unaffected by methylation of the adenine in the complementary strand, 5’-GTYGGA-3' Methylophilus methylotrophus S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine recognition sequence GATC Synechocystis sp. S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the DNA target sequence is GANTC Brucella abortus S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the DNA target sequence is GANTC, CcrM is more active on hemimethylated than unmethylated DNA Caulobacter vibrioides S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not Klebsiella pneumoniae S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not Aeromonas hydrophila S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not Salmonella enterica S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not Vibrio cholerae serotype O1 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not Yersinia enterocolitica S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not Aggregatibacter actinomycetemcomitans S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not Campylobacter jejuni S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not Pasteurella multocida S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme modifies adenine in the nickase recognition site 5'-GAGTC-3' and is specific for 5'-GASTC-3' substrates Geobacillus stearothermophilus S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the sams1 gene is methylated at an internal adenine residue of GATC site in symbiont-bearing amoebae but not in symbiont-free amoebae, suggesting that the modification may have caused the inactivation of sams1 at the transcriptional level Amoeba proteus S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the specific sequence recognized by M.NgoAXP is 5'-CCACC-3', in which the adenine residue is methylated Neisseria gonorrhoeae S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the target sequence is 5'GATC3' Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the target sequence is 5'GATC3' Yersinia pseudotuberculosis S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the target sequence is 5'GATC3' Salmonella enterica S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the target sequence is 5'GATC3' Caulobacter vibrioides S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the wild type enzyme shows target specificity for GATC sites Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine 129-mer DNA or pUC19 DNA are used as substrates Caulobacter vibrioides S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine duplex 2 DNA, 5'-GCAG-3' is the recognition site for the enzyme Helicobacter pylori S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine HP0050 methyltransferase methylates one adenine at a time in the sequence 5'-GAAG-3' Helicobacter pylori S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the EcoRV DNA methyltransferase methylates the first adenine in the GATATC recognition sequence Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates adenines at the N6 position of palindromic 5’-GATC-3’ sites. The enzyme ethylates both strands of the same site prior to fully dissociating from the DNA, a process referred to as intrasite processivity. Intrasite processivity is disrupted when the DNA flanking a single GATC site is longer than 400 bp on either side. The introduction of a second GATC site within this flanking DNA reinstates intrasite methylation of both sites Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the Hia5 protein causes the methylation of 61% of the adenines in lambda DNA Haemophilus influenzae biotype aegyptius S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the Hin1523 protein causes the methylation of 29.6% of the adenines in lambda DNA Haemophilus influenzae S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA Tetrahymena thermophila S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA Oxytricha trifallax S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates the adenine of hemimethylated GANTC after replication Caulobacter vibrioides S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site Haemophilus influenzae S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site Lactococcus cremoris S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3' Helicobacter pylori S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme complex is active on single-stranded and unpaired DNA in vitro and methylates GGACT in single-strand DNA and double-strand DNA-containing mismatches Homo sapiens S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Corynebacterium striatum M82B S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine M.HpyAXII targets GTAC sites Helicobacter pylori NSH57 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Neisseria meningitidis Z2491 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Rhodopseudomonas palustris BisB5 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site Haemophilus influenzae RD S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Neisseria meningitidis Z2491 (Pnme1) S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Deinococcus radiodurans RI S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the Hin1523 protein causes the methylation of 29.6% of the adenines in lambda DNA Haemophilus influenzae Rd (FluMu) S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Photorhabdus luminescens TT01 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the sams1 gene is methylated at an internal adenine residue of GATC site in symbiont-bearing amoebae but not in symbiont-free amoebae, suggesting that the modification may have caused the inactivation of sams1 at the transcriptional level Amoeba proteus D S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine Dam catalyzes the methylation of N-6 of the adenine residue in GATC sequences Edwardsiella tarda TXD1 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Neisseria lactamica ST640 Sange S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Rhodospirillum centenum SW S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Deinococcus radiodurans NEB479 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the Hia5 protein causes the methylation of 61% of the adenines in lambda DNA Haemophilus influenzae biotype aegyptius ATCC 11116 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the specific sequence recognized by M.NgoAXP is 5'-CCACC-3', in which the adenine residue is methylated Neisseria gonorrhoeae FA 1090 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3' Helicobacter pylori 26695 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Bacillus sp. (in: Bacteria) NEB686 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA Oxytricha trifallax JRB310 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Nitrobacter hamburgensis X14 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Synechococcus sp. PCC 7002 PR-6 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Aeromonas hydrophila SSU S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA Tetrahymena thermophila SB210 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Tetrahymena thermophila SB210 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme modifies adenine in the nickase recognition site 5'-GAGTC-3' and is specific for 5'-GASTC-3' substrates Geobacillus stearothermophilus SE-589 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site Lactococcus cremoris W15 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine - Pseudomonas sp. OM2164 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site Escherichia coli E1585-68 S-adenosyl-L-homocysteine + DNA 6-methyladenine - ? 389689 2.1.1.72 S-adenosyl-L-methionine + DNA duplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence 100% activity Escherichia coli ? - - 456653 2.1.1.72 S-adenosyl-L-methionine + DNA duplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence 100% activity Lactococcus cremoris ? - - 456653 2.1.1.72 S-adenosyl-L-methionine + DNA duplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence 100% activity Lactococcus cremoris W15 ? - - 456653 2.1.1.72 S-adenosyl-L-methionine + DNA duplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence 100% activity Escherichia coli E1585-68 ? - - 456653 2.1.1.72 S-adenosyl-L-methionine + double stranded DNA adenine - Haemophilus influenzae S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine - ? 456470 2.1.1.72 S-adenosyl-L-methionine + double stranded DNA adenine - Escherichia coli S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine - ? 456470 2.1.1.72 S-adenosyl-L-methionine + double stranded DNA adenine - Lactococcus cremoris S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine - ? 456470 2.1.1.72 S-adenosyl-L-methionine + double stranded DNA adenine - Haemophilus influenzae RD S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine - ? 456470 2.1.1.72 S-adenosyl-L-methionine + double stranded DNA adenine - Lactococcus cremoris W15 S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine - ? 456470 2.1.1.72 S-adenosyl-L-methionine + double stranded DNA adenine - Escherichia coli E1585-68 S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine - ? 456470 2.1.1.72 S-adenosyl-L-methionine + Int1 DNA adenine methylation occurs at a GAATTC site Acinetobacter baumannii S-adenosyl-L-homocysteine + Int1 DNA containing N6-methyladenine - ? 456537 2.1.1.72 S-adenosyl-L-methionine + Int1 DNA adenine methylation occurs at a GAATTC site Acinetobacter baumannii ATCC 17978 S-adenosyl-L-homocysteine + Int1 DNA containing N6-methyladenine - ? 456537 2.1.1.72 S-adenosyl-L-methionine + lambda phage DNA the enzyme modifies the adenine residue with formation of 5'-(m6A)GCT-3' Cellulosimicrobium cellulans ? - ? 456555 2.1.1.72 S-adenosyl-L-methionine + lambda phage DNA the enzyme modifies the adenine residue with formation of 5'-(m6A)GCT-3' Cellulosimicrobium cellulans B ? - ? 456555 2.1.1.72 S-adenosyl-L-methionine + mRNA - Caulobacter vibrioides S-adenosyl-L-homocysteine + mRNA containing N6-methyladenine - ? 454622 2.1.1.72 S-adenosyl-L-methionine + oligodeoxynucleotide duplexes substrate contains one single specific recognition site as 5'-GATC/5'-GATC, or modified variants Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine - ? 377720 2.1.1.72 S-adenosyl-L-methionine + pUC19 DNA adenine - Escherichia coli S-adenosyl-L-homocysteine + pUC19 DNA 6-methyladenine - ? 410274 2.1.1.72 S-adenosyl-L-methionine + RNA/DNA heteroduplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence low activity. Only the DNA strand of the RNA/DNA heteroduplex is methylated Escherichia coli ? - - 456654 2.1.1.72 S-adenosyl-L-methionine + RNA/DNA heteroduplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence low activity. Only the DNA strand of the RNA/DNA heteroduplex is methylated Lactococcus cremoris ? - - 456654 2.1.1.72 S-adenosyl-L-methionine + RNA/DNA heteroduplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence low activity. Only the DNA strand of the RNA/DNA heteroduplex is methylated Lactococcus cremoris W15 ? - - 456654 2.1.1.72 S-adenosyl-L-methionine + RNA/DNA heteroduplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence low activity. Only the DNA strand of the RNA/DNA heteroduplex is methylated Escherichia coli E1585-68 ? - - 456654 2.1.1.72 S-adenosyl-L-methionine + single stranded DNA adenine the level of single stranded DNA methylation is 7fold lower than double stranded DNA. However, upon star activity conditions (30% DMSO), the efficiency of single stranded DNA modification is raised by up to 50% of the relative activity Escherichia coli S-adenosyl-L-homocysteine + single stranded DNA 6-methyladenine - ? 456616 2.1.1.72 S-adenosyl-L-methionine + single stranded DNA adenine the level of single stranded DNA methylation is 7fold lower than double stranded DNA. However, upon star activity conditions (30% DMSO), the efficiency of single stranded DNA modification is raised by up to 50% of the relative activity Escherichia coli E1585-68 S-adenosyl-L-homocysteine + single stranded DNA 6-methyladenine - ? 456616 2.1.1.72 S-adenosyl-L-methionine + T7 DNA adenine - Geobacillus stearothermophilus S-adenosyl-L-homocysteine + T7 DNA 6-methyladenine - ? 407371 2.1.1.72 S-adenosyl-L-methionine + T7 DNA adenine - Geobacillus stearothermophilus SE-589 S-adenosyl-L-homocysteine + T7 DNA 6-methyladenine - ? 407371 2.1.1.72 S-adenosyl-L-methionine + T7 phage DNA the enzyme modifies the adenine residue with formation of 5'-(m6A)GCT-3' Cellulosimicrobium cellulans ? - ? 456622 2.1.1.72 S-adenosyl-L-methionine + T7 phage DNA the enzyme modifies the adenine residue with formation of 5'-(m6A)GCT-3' Cellulosimicrobium cellulans B ? - ? 456622