2.1.1.56 additional information ATP, XTP, CTP, UTP, and guanosine containing compounds with phospho groups in either position 2' or 3' or in 3'-5'phosphodiester linkage Vaccinia virus ? - ? 89 2.1.1.56 additional information isolated methyltransferase domain shows a lower but kinetically equivalent activity than the complete enzyme, which is highly enhanced by association with the D12 subunit Vaccinia virus ? - ? 89 2.1.1.56 additional information no substrates are poly-(A) terminating in a single phosphate or a diphosphate Homo sapiens ? - ? 89 2.1.1.56 additional information no substrates are poly-(A) terminating in a single phosphate or a diphosphate Vaccinia virus ? - ? 89 2.1.1.56 additional information no methylation of internal or conventional 5'-terminal guanosine residues Vaccinia virus ? - ? 89 2.1.1.56 additional information GDP, GTP, G(5')ppppG Homo sapiens ? - ? 89 2.1.1.56 additional information methylation of the cap structure is also implicated in polyadenylation-mediated translational activation during Xenopus oocyte maturation Xenopus laevis ? - ? 89 2.1.1.56 additional information does not methylate ATP, CTP, UTP, ITP, or m7GTP Encephalitozoon cuniculi ? - ? 89 2.1.1.56 additional information enzyme sequentially methylates the guanine N-7 and ribose 2'-O positions of viral RNA cap. The order of two methylations is determined by the preference of 2'-O methylation for substrate m7GpppA-RNA to GpppA-RNA, and the 2'-O methylation is not absolutely dependent on the prior N-7 methylation Dengue virus ? - ? 89 2.1.1.56 additional information no substrates: ATP, CTP, UTP, dATP, dCTP, dUTP or cap analog m7GpppA Severe acute respiratory syndrome-related coronavirus ? - ? 89 2.1.1.56 additional information the enzyme is responsible for guanine-N7 methylation of recapped mRNAs Homo sapiens ? - - 89 2.1.1.56 additional information no activity with 7mGpppA Homo sapiens ? - - 89 2.1.1.56 additional information no activity with 7mGpppA Severe acute respiratory syndrome-related coronavirus ? - - 89 2.1.1.56 additional information the protein reveals cap-independent methyltransferase activities targeting internal adenosine residues. Besides this, the enzyme domain also methylates the N7 position of the cap guanosine. The enzyme preferentially methylates adenosine residues and is not active on poly G, C and U RNAs Sudan ebolavirus ? - - 89 2.1.1.56 additional information ATP, XTP, CTP, UTP, and guanosine containing compounds with phospho groups in either position 2' or 3' or in 3'-5'phosphodiester linkage Vaccinia virus WR ? - ? 89 2.1.1.56 additional information no substrates are poly-(A) terminating in a single phosphate or a diphosphate Vaccinia virus WR ? - ? 89 2.1.1.56 S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] - Homo sapiens S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] - ? 454497 2.1.1.56 S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] - Murine hepatitis virus S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] - ? 454497 2.1.1.56 S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] - Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] - ? 454497 2.1.1.56 S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] the enzyme methylates the guanosine cap on the N-7 position to create the cap structure, m7G(5)ppp(5)X Homo sapiens S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] - ? 454497 2.1.1.56 S-adenosyl-L-methionine + dGTP - Encephalitozoon cuniculi S-adenosyl-L-homocysteine + m7dGTP - ? 389684 2.1.1.56 S-adenosyl-L-methionine + dGTP and G(5')pppG, best substrates Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + m7dGTP - ? 389684 2.1.1.56 S-adenosyl-L-methionine + G(5')pppA - Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + m7G(5')pppA - ? 434228 2.1.1.56 S-adenosyl-L-methionine + G(5')pppG and dGTP, best substrates Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + m7G(5')pppG - ? 434229 2.1.1.56 S-adenosyl-L-methionine + G(5')pppN N: G Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppG - ? 319763 2.1.1.56 S-adenosyl-L-methionine + G(5')pppN N: G Neurospora crassa S-adenosyl-L-homocysteine + m7G(5')pppG - ? 319763 2.1.1.56 S-adenosyl-L-methionine + G(5')pppN N: G bluetongue virus S-adenosyl-L-homocysteine + m7G(5')pppG - ? 319763 2.1.1.56 S-adenosyl-L-methionine + G(5')pppN N: A Neurospora crassa S-adenosyl-L-homocysteine + m7G(5')pppG - ? 319763 2.1.1.56 S-adenosyl-L-methionine + G(5')pppN N: A Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppG - ? 319763 2.1.1.56 S-adenosyl-L-methionine + G(5')pppN unmethylated cap analogue, serves as in vitro substrate bluetongue virus S-adenosyl-L-homocysteine + m7G(5')pppG - ? 319763 2.1.1.56 S-adenosyl-L-methionine + G(5')pppN TbCmt1 Trypanosoma brucei S-adenosyl-L-homocysteine + m7G(5')pppG - ? 319763 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - Dengue virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - Semliki forest virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - bluetongue virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - Saccharomyces cerevisiae S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - Xenopus laevis S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - West Nile virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - vesicular stomatitis virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - Wesselsbron virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - Mus musculus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - Avian orthoavulavirus 1 S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - Semliki forest virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA unmethylated mRNA of reovirus and poly A(+) RNA Neurospora crassa S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA acceptor substrate specificity, overview Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA transfers methyl group from S-adenosyl-L-methionine specifically to position 7 of terminal guanosine Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA utilizes GMP, GDP, guanosine, ITP as substrates Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA methylates also undermethylated poly(A)-rich mRNA Saccharomyces cerevisiae S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA mRNA, synthetic polyribonucleotides terminating in a dinucleoside triphosphate Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA mRNA, synthetic polyribonucleotides terminating in a dinucleoside triphosphate Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA specific for mRNAs with 5'-terminal sequence G(5')pppA- and G(5')pppG- Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA specific for mRNAs with 5'-terminal sequence G(5')pppA- and G(5')pppG- Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA active also on E. coli tRNA Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA free G(5')pppG is utilized, G(5')ppG is a poor substrate, while GTP, GDP, and G(5')ppppG are no substrates Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA the presence of the small one is required for methylase activity, the large subunit bears functional domains for binding of the small subunit and for methylase activity Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA guanylyltransferase and RNA-triphosphatase are located on the large subunit Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA one of a series of three reactions in capping of vaccinia virus mRNA catalyzed by one enzyme, which is a heterodimer: no methylase activity with one of the subunits alone Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA in infected BHK cells Semliki forest virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA associated with mRNA-guanylyltransferase Neurospora crassa S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA R may be guanosine or adenosine, involved in synthesis of methylated capped structure at 5'-terminal of viral mRNA Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA essential and defining step in the eukaryotic mRNA synthesis Encephalitozoon cuniculi S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA essential for cell viability Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA enzyme contains the conserved S-adenosyl-L-methionine-binding motif VLDLGXGXG, structural features for cap guanine specificity Encephalitozoon cuniculi S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA transfers methyl group from S-adenosyl-L-methionine specifically to position 7 of terminal guanosine Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA acceptor substrate specificity, overview Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA utilizes GMP, GDP, guanosine, ITP as substrates Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA mRNA, synthetic polyribonucleotides terminating in a dinucleoside triphosphate Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA specific for mRNAs with 5'-terminal sequence G(5')pppA- and G(5')pppG- Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA - Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA R may be guanosine or adenosine, involved in synthesis of methylated capped structure at 5'-terminal of viral mRNA Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA - ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA methylates also undermethylated poly(A)-rich mRNA Saccharomyces cerevisiae VY1160 S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ? 319762 2.1.1.56 S-adenosyl-L-methionine + GDP - Encephalitozoon cuniculi S-adenosyl-L-homocysteine + m7GDP - ? 389693 2.1.1.56 S-adenosyl-L-methionine + Gppp-RNA - Homo sapiens S-adenosyl-L-homocysteine + m7Gppp-RNA - ? 456491 2.1.1.56 S-adenosyl-L-methionine + GpppA - Saccharomyces cerevisiae S-adenosyl-L-homocysteine + m7GpppA - ? 389696 2.1.1.56 S-adenosyl-L-methionine + GpppA - Encephalitozoon cuniculi S-adenosyl-L-homocysteine + m7GpppA - ? 389696 2.1.1.56 S-adenosyl-L-methionine + GpppA TbCmt1 Trypanosoma brucei S-adenosyl-L-homocysteine + m7GpppA - ? 389696 2.1.1.56 S-adenosyl-L-methionine + GpppA-RNA - Dengue virus S-adenosyl-L-homocysteine + m7GpppA-RNA - ? 421375 2.1.1.56 S-adenosyl-L-methionine + GpppAC3 is efficiently methylated at the guanine-N7 position Homo sapiens S-adenosyl-L-homocysteine + m7GpppAC3 - ? 389695 2.1.1.56 S-adenosyl-L-methionine + GpppAC4 - Homo sapiens S-adenosyl-L-homocysteine + 7mGpppAC4 - ? 456492 2.1.1.56 S-adenosyl-L-methionine + GpppAC4 - Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + 7mGpppAC4 - ? 456492 2.1.1.56 S-adenosyl-L-methionine + GpppG - Vaccinia virus S-adenosyl-L-homocysteine + m7GpppG - ? 389697 2.1.1.56 S-adenosyl-L-methionine + GpppG TbCmt1 Trypanosoma brucei S-adenosyl-L-homocysteine + m7GpppG - ? 389697 2.1.1.56 S-adenosyl-L-methionine + GpppGAU-[RNA] strong activity Sudan ebolavirus ? - ? 456493 2.1.1.56 S-adenosyl-L-methionine + GTP - Vaccinia virus S-adenosyl-L-homocysteine + m7GTP - ? 319764 2.1.1.56 S-adenosyl-L-methionine + GTP - bamboo mosaic virus S-adenosyl-L-homocysteine + m7GTP - ? 319764 2.1.1.56 S-adenosyl-L-methionine + GTP - Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + m7GTP - ? 319764 2.1.1.56 S-adenosyl-L-methionine + GTP - Encephalitozoon cuniculi S-adenosyl-L-homocysteine + m7GTP - ? 319764 2.1.1.56 S-adenosyl-L-methionine + GTP - Chikungunya virus S-adenosyl-L-homocysteine + m7GTP - ? 319764 2.1.1.56 S-adenosyl-L-methionine + GTP viral, not host enzyme Semliki forest virus S-adenosyl-L-homocysteine + m7GTP - ? 319764 2.1.1.56 S-adenosyl-L-methionine + guanylimidodiphosphate - bamboo mosaic virus ? - ? 319766 2.1.1.56 S-adenosyl-L-methionine + guanylylimidodiphosphate - Chikungunya virus ? - ? 456532 2.1.1.56 S-adenosyl-L-methionine + m7G(5')pppG - Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + ? - ? 434252 2.1.1.56 S-adenosyl-L-methionine + mGpppGAU-[RNA] strong activity Sudan ebolavirus ? - ? 456571 2.1.1.56 S-adenosyl-L-methionine + mGpppGmAmU-[RNA] strong activity Sudan ebolavirus ? - ? 456572 2.1.1.56 S-adenosyl-L-methionine + mGpppGmAU-[RNA] strong activity Sudan ebolavirus ? - ? 456573 2.1.1.56 S-adenosyl-L-methionine + pp(A)n - Vaccinia virus ? - ? 319765