2.1.1.42 4 S-adenosyl-L-methionine + quercetin - Citrus depressa 4 S-adenosyl-L-homocysteine + 3,3',5,7-tetra-O-methyl-quercetin - ? 455025 2.1.1.42 luteolin + S-adenosyl-L-methionine high 3'-O-methyltransferase activity Glycine max 3'-O-methyl luteolin + S-adenosyl-L-homocysteine i.e. chrysoeriol ? 389110 2.1.1.42 luteolin + S-adenosyl-L-methionine OMT-15 shows 92% activity and OMT-17 shows 84% activity compared to myricetin Oryza sativa 3'-O-methylluteolin + S-adenosyl-L-homocysteine - ? 409782 2.1.1.42 additional information - Petroselinum crispum ? - ? 89 2.1.1.42 additional information isomeric substances isoferulic acid and diosmetin, chrysoeriol, apigenin p-cumaric acid and ferulic acid are not substrates Petroselinum crispum ? - ? 89 2.1.1.42 additional information only ortho-dihydric phenols can function as substrates and only the meta-hydroxyl group is methylated, no reaction takes place if one of the two phenolic hydroxyls is methylated or if only one hydroxyl group is present Petroselinum crispum ? - ? 89 2.1.1.42 additional information rutin is no substrate Nicotiana tabacum ? - ? 89 2.1.1.42 additional information methylation proceedes only in the meta-position, enzyme is unable to catalyze the methylation of p-coumaric acid, m-coumaric acid, ferulic acid, isoferulic acid, sinapic acid, apigenin and naringenin Glycine max ? - ? 89 2.1.1.42 additional information isoflavones biochanin A and daidzein do not serve as substrates Glycine max ? - ? 89 2.1.1.42 additional information SOMT-9 transfers a methyl group to the 3'-OH group of substrates having ortho-hydroxyl groups and uses a flavonoids as a substrates, no activity with 3,3'-dihydroxyflavone, 5,3'-dihydroxyflavone, 6,3'-dihydroxyflavone, 3'-hydroxyflavone, and 3'-hydroxyflavanone Glycine max ? - ? 89 2.1.1.42 additional information OMT-15 and -17 cannot utilize naringenin, apigenin, or kaempferol as substrates, taxifolin and eriodictyol do not serve as methyl acceptors Oryza sativa ? - ? 89 2.1.1.42 additional information only flavonols with vicinal B-ring hydroxyl groups, such as quercetin and myricetin are good methyl acceptor Arabidopsis thaliana ? - ? 89 2.1.1.42 additional information enzyme additionally shows N-acetylserotonin methyltransferase activity, reaction of EC 2.1.1.4, and caffeic acid methyltransferase activity, reaction of EC 2.1.1.68. The catalytic efficiency for N-acetylserotonin methyltransferase activity is 709fold lower than for caffeic acid methyltransferase. In vitro, N-acetylserotonin methyltransferase activity is dramatically decreased by the addition of caffeic acid in a dose-dependent manner, but the activity of caffeic acid methyltransferase is not altered by N-acetylserotonin Arabidopsis thaliana ? - ? 89 2.1.1.42 additional information enzyme shows methylation activity for the 3-, 5-, 6-, and 7-hydroxyl groups of flavones. The highest activity is observed for 3-hydroxyflavone (flavonol), followed by 7-hydroxyflavone with a relative activity of 15.6% of that of 3-hydroxyflavone and 5-hydroxyflavone with that of 13.5%. Very weak activity is observed for 6-hydroxyflavone and no activity for 3'- or 4'-hydroxyflavone and 7-methoxy-8-hydroxyflavone. FOMT5 does not exhibit a sequential methylation order for 3-, 5-, and 7-hydroxyl groups of quercetin. No detection of polymethylated products from naringenin, (-)-epicatechin, and equol, and cyanidin is not a substrate Citrus depressa ? - - 89 2.1.1.42 quercetin + S-adenosyl-L-methionine SOMT-9 is involved in the biosynthesis of isorhamnetin Glycine max 3'-O-methylquercetin + S-adenosyl-L-homocysteine - ? 386676 2.1.1.42 quercetin + S-adenosyl-L-methionine a flavone, 3'-O-methyltransferase activity, best substrate Glycine max 3'-O-methylquercetin + S-adenosyl-L-homocysteine i.e. isorhamnetin ? 386676 2.1.1.42 S-adenosyl-L-homocysteine + 6,7-dihydroxy-3'-methoxyisoflavone - Glycine max ? - ? 451861 2.1.1.42 S-adenosyl-L-methionine + (-)-epicatechin - Citrus depressa S-adenosyl-L-homocysteine + ? - ? 456354 2.1.1.42 S-adenosyl-L-methionine + (2S)-naringenin - Citrus depressa S-adenosyl-L-homocysteine + ? - ? 456355 2.1.1.42 S-adenosyl-L-methionine + 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-2,3-dihydro-4H-chromen-4-one - Petroselinum crispum ? - ? 451862 2.1.1.42 S-adenosyl-L-methionine + 3,3',4',5,5',7-hexahydroxyflavone i.e. mycricetin. 70% of the activity with quercetin Arabidopsis thaliana S-adenosyl-L-homocysteine + 3,4',5,5',7-pentahydroxy-3'-methoxyflavone - ? 415223 2.1.1.42 S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxy flavone quercetin Nicotiana tabacum S-adenosyl-L-homocysteine + isorhamnetin - ? 319718 2.1.1.42 S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxy flavone quercetin Glycine max S-adenosyl-L-homocysteine + isorhamnetin - ? 319718 2.1.1.42 S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone i.e. quercetin. Preferred substrate Arabidopsis thaliana S-adenosyl-L-homocysteine + 3,4',5,7-tetrahydroxy-3'-methoxyflavone i.e. isorhamnetin ? 413572 2.1.1.42 S-adenosyl-L-methionine + 4,5-dihydroxy-3-methoxycinnamate 5-hydroxy ferulic acid Nicotiana tabacum S-adenosyl-L-homocysteine + 4-hydroxy-3,5-dimethoxycinnamate - ? 319717 2.1.1.42 S-adenosyl-L-methionine + 4,5-dihydroxy-3-methoxycinnamate 5-hydroxy ferulic acid Glycine max S-adenosyl-L-homocysteine + 4-hydroxy-3,5-dimethoxycinnamate - ? 319717 2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavanone eriodictyol Glycine max S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavanone - ? 319716 2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavanone eriodictyol Petroselinum crispum S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavanone - ? 319716 2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone luteolin Glycine max S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone - ? 319713 2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone luteolin Petroselinum crispum S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone - ? 319713 2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone luteolin Petroselinum crispum S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone chrysoeriol ? 319713 2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone luteolin, enzyme directly related to flavone glycoside biosynthesis, biosynthesis of 3'-methoxyflavonoids Petroselinum crispum S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone chrysoeriol ? 319713 2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone i.e. luteolin. 15% of the activity with quercetin Arabidopsis thaliana S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone - ? 319713 2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone i.e. luteolin, the enzyme also catalyzes the methylation of tricetin and the methylation of quercetin, prefers tricetin over luteolin Hordeum vulgare S-adenosyl-L-homocysteine + 3'-O-methylluteolin - ? 410197 2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone i.e. lutepolin, the enzyme also catalyzes the methylation of tricetin and the methylation of 5-hydroxyferulic acid and quercetin, prefers tricetin over luteolin Zea mays S-adenosyl-L-homocysteine + 3'-O-methylluteolin - ? 410197 2.1.1.42 S-adenosyl-L-methionine + 5,7,3'-trihydroxyflavone - Petroselinum crispum ? - ? 451863 2.1.1.42 S-adenosyl-L-methionine + 5,7,3'-trihydroxyflavone 7-O-glucoside - Glycine max S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone 7-O-glucoside chrysoeriol-7-O-glucoside ? 319714 2.1.1.42 S-adenosyl-L-methionine + 5,7,3'-trihydroxyflavone 7-O-glucoside - Petroselinum crispum S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone 7-O-glucoside chrysoeriol-7-O-glucoside ? 319714 2.1.1.42 S-adenosyl-L-methionine + 5,7,3'-trihydroxyflavone 7-O-glucoside luteolin 7-O-glucoside Petroselinum crispum S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone 7-O-glucoside chrysoeriol-7-O-glucoside ? 319714 2.1.1.42 S-adenosyl-L-methionine + 5-hydroxyferulic acid OMT-15 shows 25% activity and OMT-17 shows 18% activity compared to myricetin Oryza sativa ? - ? 410198 2.1.1.42 S-adenosyl-L-methionine + 7,8-dihydroxyflavone OMT-15 shows 102% activity and OMT-17 shows 143% activity compared to myricetin Oryza sativa ? - ? 410202 2.1.1.42 S-adenosyl-L-methionine + dihydro-3,3',4',5,7-pentahydroxy flavone dihydroquercetin Nicotiana tabacum S-adenosyl-L-homocysteine + dihydroisorhamnetin - ? 319719 2.1.1.42 S-adenosyl-L-methionine + dihydro-3,3',4',5,7-pentahydroxy flavone dihydroquercetin Glycine max S-adenosyl-L-homocysteine + dihydroisorhamnetin - ? 319719 2.1.1.42 S-adenosyl-L-methionine + equol - Citrus depressa S-adenosyl-L-homocysteine + ? - ? 456474 2.1.1.42 S-adenosyl-L-methionine + quercetin - Petroselinum crispum S-adenosyl-L-homocysteine + 3'-O-methylquercetin - ? 424070 2.1.1.42 S-adenosyl-L-methionine + quercetin 3-O-glucoside poor substrate Glycine max S-adenosyl-L-homocysteine + ? - ? 319720 2.1.1.42 S-adenosyl-L-methionine + rutin poor substrate Glycine max S-adenosyl-L-homocysteine + ? - ? 319721 2.1.1.42 taxifolin + S-adenosyl-L-methionine 3'-O-methyltransferase activity Glycine max 3'-O-methyl taxifolin + S-adenosyl-L-homocysteine - ? 389851