2.1.1.274 additional information GC-MS product identification Jasminum sambac ? - - 89 2.1.1.274 additional information enzyme is also active with benzoic acid resulting in methyl benzoate formation Antirrhinum majus ? - ? 89 2.1.1.274 additional information enzyme is also active with benzoic acid resulting in methyl benzoate formation Arabidopsis thaliana ? - ? 89 2.1.1.274 additional information enzyme is also active with benzoic acid resulting in methyl benzoate formation Arabidopsis lyrata ? - ? 89 2.1.1.274 additional information enzyme is also active with benzoic acid resulting in methyl benzoate formation Clarkia breweri ? - ? 89 2.1.1.274 additional information in contrast to AtBSMT1 no activity with 1 mM 3-hydroxybenzoic acid Arabidopsis lyrata ? - ? 89 2.1.1.274 additional information no activity with 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, trans-cinnamic acid, p-coumaric acid, m-coumaric acid, o-coumaric acid and benzyl alcohol Antirrhinum majus ? - ? 89 2.1.1.274 additional information no activity with 4-hydroxybenzoic acid, vanillic acid, caffeic acid, p-coumaric acid and (+/-)jasmonic acid Clarkia breweri ? - ? 89 2.1.1.274 additional information The enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.273, benzoate O-methyltransferase Arabidopsis thaliana ? - ? 89 2.1.1.274 additional information The enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.273, benzoate O-methyltransferase Arabidopsis lyrata ? - ? 89 2.1.1.274 additional information jasmonic acid, indole-3-acetic acid and gibberellic acid do not serve as substrates for isoform SAMT1 Glycine max ? - ? 89 2.1.1.274 additional information less than 1% activity with jasmonic acid, indole-3-acetic acid and gibberellic acid 3 Solanum lycopersicum ? - ? 89 2.1.1.274 additional information no measurable methylation of jasmonic acid by wild-type SAMT using concentrations up to 5 mM Clarkia breweri ? - ? 89 2.1.1.274 additional information wild-type is also able to methylate benzoic acid with 48% activity compared to salicylate methylation Clarkia breweri ? - ? 89 2.1.1.274 additional information the enzyme also catalyzes the reaction of benzoic acid carboxyl methyltransferase, EC 2.1.1.273 Lilium hybrid cultivar ? - ? 89 2.1.1.274 additional information the enzyme also catalyzes the reaction of benzoic acid carboxyl methyltransferase, EC 2.1.1.273 Plasmodiophora brassicae ? - ? 89 2.1.1.274 additional information development and evaluation of an enzyme-coupled assay for monitoring methyltransferase activity, overview. Since S-adenosyl-L-homocysteine is a key by-product of reactions catalyzed by S-adenosyl methionine-dependent methyltransferases, the coupling enzymes are used to assess the activities of EcoRI methyltransferase and a salicylic acid methyltransferase from Clarkia breweri in the presence of S-adenosyl methionine. In the case of the salicylic acid methyltransferase, detectable activity is observed for several substrates including salicylic acid, benzoic acid, 3-hydroxybenzoic acid, and vanillic acid, substrate specificity, overview. Additionally, the de novo synthesis of the relatively expensive and unstable cosubstrate, S-adenosyl methionine, catalyzed by methionine adenosyltransferase can be incorporated within the assay. The assay offers a high level of sensitivity that permits continuous and reliable monitoring of methyltransferase activities. The assay enzymes, 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (Mtn), xanthine oxidase (XOD), and horse radish peroxidase (HRP), are able to operate in a tandem manner to generate a fluorescence signal in the presence of SAH, the key by-product of reactions catalyzed by SAM-dependent methyltransferases. Poor or no substrates are acetate, propanoate, butyrate, 4-hydroxybenzoate, jasmonate, cinnamate, coumarate, and caffeate Clarkia breweri ? - - 89 2.1.1.274 additional information enzyme PtSABATH4 exhibits high enzymatic activity towards the substrate salicylate (SA) and weak activity towards benzoic acid, jasmonic acid, and farnesoic acid. PtSABATH4 does not show any activity towards indole-3-acetic acid, vanillic acid, nicotinic acid, coumalic acid, and trans-cinnamic acid. PtSABATH4 shows at least 4.3fold higher enzymatic activity towards the substrate SA. PtSABATH4 displays the highest level of catalytic activity towards SA and a relatively low level of activity towards BA. Preference for salicylic acid (SA) over benzoic acid (BA) in wild-type PtSABATH4 and preference for BA over SA in the PtSABATH4 M156H mutant Populus trichocarpa ? - - 89 2.1.1.274 additional information isozyme PaSABATH2 has the highest level of specific activity with salicylic acid and is designated as PaSAMT (EC 2.1.1.274). For comparison, PaSAMT is also assayed with two compounds of similar structure benzoic acid and anthranilic acid (cf. EC 2.1.1.273). While PaSAMT has no activity with anthranilic acid, its activity with benzoic acid is approximately 8% of that with salicylic acid. Radiochemical assay method Picea abies ? - - 89 2.1.1.274 additional information substrate specificity of recombinant enzyme, overview Camellia sinensis ? - - 89 2.1.1.274 S-adenosyl-L-methionine + 3-hydroxybenzoate 1.8% activity compared to salicylate Glycine max S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate - ? 425002 2.1.1.274 S-adenosyl-L-methionine + 3-hydroxybenzoate 26% activity compared to salicylate Clarkia breweri methyl 3-hydroxybenzoate + S-adenosyl-L-homocysteine - ? 456385 2.1.1.274 S-adenosyl-L-methionine + 3-hydroxybenzoic acid 54% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate - ? 424033 2.1.1.274 S-adenosyl-L-methionine + 3-hydroxybenzoic acid less than 2% relative activity at 1 mM methyl acceptor compared to activity with salicylate set at 100% Clarkia breweri S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate - ? 424033 2.1.1.274 S-adenosyl-L-methionine + 3-hydroxybenzoic acid 17% activity of wild-type enzyme compared to salicylate methylation Clarkia breweri S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate - ? 424033 2.1.1.274 S-adenosyl-L-methionine + 4-aminosalicylate 2% activity compared to salicylate Solanum lycopersicum S-adenosyl-L-homocysteine + methyl 4-aminosalicylate - ? 425004 2.1.1.274 S-adenosyl-L-methionine + 4-hydroxybenzoate lower catalytic efficiency with 4-hydroxybenzoate compared to salicylate Clarkia breweri S-adenosyl-L-homocysteine + methyl 4-hydroxybenzoate - ? 425005 2.1.1.274 S-adenosyl-L-methionine + anthranilate 9.3% activity compared to salicylate Glycine max ? - ? 425014 2.1.1.274 S-adenosyl-L-methionine + anthranilate - Plasmodiophora brassicae methyl anthranilate + S-adenosyl-L-homocysteine - ? 456410 2.1.1.274 S-adenosyl-L-methionine + anthranilic acid 32% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis lyrata S-adenosyl-L-homocysteine + methyl anthranilate - ? 424037 2.1.1.274 S-adenosyl-L-methionine + anthranilic acid 35% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl anthranilate - ? 424037 2.1.1.274 S-adenosyl-L-methionine + benzoate 2% activity compared to salicylate Solanum lycopersicum S-adenosyl-L-homocysteine + methyl benzoate - ? 424038 2.1.1.274 S-adenosyl-L-methionine + benzoate 16.9% activity compared to salicylate Glycine max ? - ? 425015 2.1.1.274 S-adenosyl-L-methionine + benzoate - Picea abies methyl benzoate + S-adenosyl-L-homocysteine - ? 454518 2.1.1.274 S-adenosyl-L-methionine + benzoate - Brassica oleracea var. capitata methyl benzoate + S-adenosyl-L-homocysteine - ? 454518 2.1.1.274 S-adenosyl-L-methionine + benzoate - Arabidopsis thaliana methyl benzoate + S-adenosyl-L-homocysteine - ? 454518 2.1.1.274 S-adenosyl-L-methionine + benzoate - Plasmodiophora brassicae methyl benzoate + S-adenosyl-L-homocysteine - ? 454518 2.1.1.274 S-adenosyl-L-methionine + benzoate - Populus trichocarpa methyl benzoate + S-adenosyl-L-homocysteine - ? 454518 2.1.1.274 S-adenosyl-L-methionine + benzoate 8% activity compared to salicylate Picea abies methyl benzoate + S-adenosyl-L-homocysteine - ? 454518 2.1.1.274 S-adenosyl-L-methionine + benzoate 96% activity compared to salicylate Clarkia breweri methyl benzoate + S-adenosyl-L-homocysteine - ? 454518 2.1.1.274 S-adenosyl-L-methionine + benzoate - Arabidopsis thaliana Col-0 methyl benzoate + S-adenosyl-L-homocysteine - ? 454518 2.1.1.274 S-adenosyl-L-methionine + cinnamic acid less than 2% relative activity at 1 mM methyl acceptor compared to activity with salicylate set at 100% Clarkia breweri S-adenosyl-L-homocysteine + methyl cinnamate - ? 424043 2.1.1.274 S-adenosyl-L-methionine + jasmonic acid Y147S/M150H double mutant and Y147S/M150H/F347Y triple mutant are able to turn over jasmonic acid, while preserving substantial salicylate methylating activity Clarkia breweri S-adenosyl-L-homocysteine + methyl jasmonate - ? 427026 2.1.1.274 S-adenosyl-L-methionine + salicylate - Atropa belladonna S-adenosyl-L-homocysteine + methyl salicylate - ? 422067 2.1.1.274 S-adenosyl-L-methionine + salicylate - Clarkia breweri S-adenosyl-L-homocysteine + methyl salicylate - ? 422067 2.1.1.274 S-adenosyl-L-methionine + salicylate - Nicotiana benthamiana S-adenosyl-L-homocysteine + methyl salicylate - ? 422067 2.1.1.274 S-adenosyl-L-methionine + salicylate - Lilium hybrid cultivar S-adenosyl-L-homocysteine + methyl salicylate - ? 422067 2.1.1.274 S-adenosyl-L-methionine + salicylate - Plasmodiophora brassicae S-adenosyl-L-homocysteine + methyl salicylate - ? 422067 2.1.1.274 S-adenosyl-L-methionine + salicylate - Solanum lycopersicum S-adenosyl-L-homocysteine + methyl salicylate - ? 422067 2.1.1.274 S-adenosyl-L-methionine + salicylate 100% activity Solanum lycopersicum S-adenosyl-L-homocysteine + methyl salicylate - ? 422067 2.1.1.274 S-adenosyl-L-methionine + salicylate highest level of specific activity with salicylate Glycine max S-adenosyl-L-homocysteine + methyl salicylate - ? 422067 2.1.1.274 S-adenosyl-L-methionine + salicylate - Picea abies methyl salicylate + S-adenosyl-L-homocysteine - ? 454631 2.1.1.274 S-adenosyl-L-methionine + salicylate - Brassica oleracea var. capitata methyl salicylate + S-adenosyl-L-homocysteine - ? 454631 2.1.1.274 S-adenosyl-L-methionine + salicylate - Arabidopsis thaliana methyl salicylate + S-adenosyl-L-homocysteine - ? 454631 2.1.1.274 S-adenosyl-L-methionine + salicylate - Clarkia breweri methyl salicylate + S-adenosyl-L-homocysteine - ? 454631 2.1.1.274 S-adenosyl-L-methionine + salicylate - Plasmodiophora brassicae methyl salicylate + S-adenosyl-L-homocysteine - ? 454631 2.1.1.274 S-adenosyl-L-methionine + salicylate - Populus trichocarpa methyl salicylate + S-adenosyl-L-homocysteine - ? 454631 2.1.1.274 S-adenosyl-L-methionine + salicylate - Jasminum sambac methyl salicylate + S-adenosyl-L-homocysteine - ? 454631 2.1.1.274 S-adenosyl-L-methionine + salicylate - Camellia sinensis methyl salicylate + S-adenosyl-L-homocysteine - ? 454631 2.1.1.274 S-adenosyl-L-methionine + salicylate - Lycium chinense methyl salicylate + S-adenosyl-L-homocysteine - ? 454631 2.1.1.274 S-adenosyl-L-methionine + salicylate best substrate Picea abies methyl salicylate + S-adenosyl-L-homocysteine - ? 454631 2.1.1.274 S-adenosyl-L-methionine + salicylate best substrate Clarkia breweri methyl salicylate + S-adenosyl-L-homocysteine - ? 454631 2.1.1.274 S-adenosyl-L-methionine + salicylate - Arabidopsis thaliana Col-0 methyl salicylate + S-adenosyl-L-homocysteine - ? 454631 2.1.1.274 S-adenosyl-L-methionine + salicylic acid - Antirrhinum majus S-adenosyl-L-homocysteine + methyl salicylate - ? 424073 2.1.1.274 S-adenosyl-L-methionine + salicylic acid 16% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis lyrata S-adenosyl-L-homocysteine + methyl salicylate - ? 424073 2.1.1.274 S-adenosyl-L-methionine + salicylic acid 46% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl salicylate - ? 424073 2.1.1.274 S-adenosyl-L-methionine + vanillate 12% activity compared to salicylate Clarkia breweri methyl vanillate + S-adenosyl-L-homocysteine - ? 456633 2.1.1.274 S-adenosyl-L-methionine + vanillic acid 5.1% activity of wild-type enzyme compared to salicylate methylation Clarkia breweri S-adenosyl-L-homocysteine + methyl 4-hydroxy-3-methoxybenzoate - ? 427028