2.1.1.246	a [methyl-Co(III) methanol-specific corrinoid protein] + 3-mercaptopropanoic acid	-	Methanosarcina barkeri	methyl-3-mercaptopropanoic acid + a [Co(I) methanol-specific corrinoid protein]	-	r	418345	
2.1.1.246	a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M	-	Methanosarcina barkeri	methyl-CoM + a [Co(I) methanol-specific corrinoid protein]	-	?	417877	
2.1.1.246	a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M	-	Methanosarcina barkeri	methyl-CoM + a [Co(I) methanol-specific corrinoid protein]	-	r	417877	
2.1.1.246	a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M	the enzyme methylcobamide:CoM methyltransferase, MT2, catalyzes the transfer of the methyl group from the MT1-bound methylcobamide prosthetic group to coenzyme M	Methanosarcina barkeri	methyl-CoM + a [Co(I) methanol-specific corrinoid protein]	-	r	417877	
2.1.1.246	a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M	-	Methanosarcina barkeri Fusaro, DSM 804	methyl-CoM + a [Co(I) methanol-specific corrinoid protein]	-	r	417877	
2.1.1.246	a [methyl-Co(III) methanol-specific corrinoid protein] + coenzyme M	-	Methanosarcina barkeri Fusaro / DSM 804	methyl-CoM + a [Co(I) methanol-specific corrinoid protein]	-	r	417877	
2.1.1.246	a [methyl-Co(III) methanol-specific corrinoid protein] + CoM	-	Methanosarcina barkeri	methyl-CoM + a [Co(I) methanol-specific corrinoid protein]	-	?	454233	
2.1.1.246	a [methyl-Co(III) methanol-specific corrinoid protein] + CoM	-	Methanosarcina barkeri	methyl-CoM + a [Co(I) methanol-specific corrinoid protein]	-	r	454233	
2.1.1.246	additional information	 transferase MtaA catalyzes the transfer of the methyl group from methylated transferase MtaBC to coenzyme M. Instead of methylated transferase MtaBC, MtaA can also use free methylcobalamin as the methyl donor. MtaA activates coenzyme M for nucleophilic attack of the methyl group of the methylcobamide	Methanosarcina barkeri	?	-	?	89	
2.1.1.246	additional information	conversions of monomethylamine and dimethylamine to CH3-SCoM are dependent upon MT2-A, and are not supported by MT2-M. In contrast, MT2-M acts specifically in metabolism of methanol, but does not substitute for MT2-A in conversion of monomethylamine or dimethylamine. Nevertheless, both isozymes are capable of supporting the conversion of trimethylamine	Methanosarcina barkeri	?	-	?	89	
2.1.1.246	additional information	methyltransferase 2, MT2, transfers the methyl group from the methylated corrinoid protein to coenzyme M, i.e. 2-mercaptoethanesulfonic acid. Methyl-coenzyme M is then disproportionated, with one molecule being oxidized to CO2 to provide the six electrons required for reduction of three additional methyl-coenzyme M molecules to methane	Methanosarcina acetivorans	?	-	?	89	
2.1.1.246	additional information	in the assay for methanol:coenzyme M methyltransferase activity cob(I)alamin can be substituted by cob(I)inamide which is devoid of the nucleotide loopmethylation of cob(I)inamide with methanol is dependent on imidazole but not on the demethylation of methylcob(III)inamide with coenzyme M	Methanosarcina barkeri	?	-	?	89	
2.1.1.246	additional information	recombinant MtaA catalyzes the formation of methyl-coenzyme M from free CH3-cob(III)alamin and coenzyme M at specific rates comparable to those predicted for MtaA-catalyzed MtaC demethylation	Methanosarcina barkeri	?	-	?	89	
2.1.1.246	additional information	the reaction for possible production of methanol from the anaerobic oxidation of methane can be reversed in vitro. Develoment of an in vitro functional assay that demonstrates MtaABC can catalyze the energetically unfavorable reverse reaction starting from methyl coenzyme M and generating methanol as a product, overview. MtaABC enzyme protein complex to catalyze the methyl transfer from methanol to CoM to form methyl-CoM, which is energetically favorable in the forward reaction. MtaB catalyzes a methyl transfer from methanol to the corrinoid cofactor of the MtaC subunit. MtaA then catalyzes the transfer of the methyl group to CoM to form methyl-CoM. DTNB assays for the forward and reverse MtaABC reactions. Methylcobalamin assay of purified recombinant MtaA.Demethylation of methylcobalamin in a CoM-dependent manner by MtaA	Methanosarcina barkeri	?	-	-	89